RchiOBHm_Chr2g0136781

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000338
Physical Location & Seq
Forward (+)
54078223 .. 54083296
5074 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50756

Sequence Viewer

Length: 1671 bp
ATGTGTTTTTACATTGCTAGGTTGAAATTTGGAGAAGCGAAGCTTAGAGCTTACAGAGAAATGGATGGAAGTACTTCTGGTGTAGAAGACAATGATATTGATTGGAACACAGAAGATGAGCTTGAATTGGACAACTTCACTTTATCTCCTTGTTCTAGTTTGCTACTTACTGGTGGCGAAGCTAATGCCTTCTCTGGGGAGGCGAGCTCATGTCCAAGTTCTTCCAACTCCAAAGTTCTTGATCAGTTTATAGGGATGGGATTTTCTAAGAAGATGGTTGAAAAAGCAATTCAGGAACATGATTTGGAAAGCTCTGGTGATGAGGAAATCATGAGTCCTACATCCGAGGAGGAAAAGCTACTCATGTCCTTGATCAAAATGGGGTACACAGAAGAGGAGGCTTCCATAGCTATAGAAAGATGTGGTCTTAGCTCTTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCATTTGGCAAAGGCAGAAGATGCCCTTCTTCCTCTAGAACAGAAGCCTGGACTAAAACATTTGCACACTGATTATCCTAAGAAGAAAAGGAAACTTTTGGTTAATCAAGTGTCGAAAAGGAAAAGGCAAAATAGGCTCATAGGCGAGGACAATGAGACAGTTCGTCTCCCAAATCCAATGATTGGGTTTGGTATTCCAACTGGACCATGCCAATCTCACAGAACTCTTCCAAAGGCAGCCATAGGACCTCCCTATTTTTATTTTGAGAATGTAGCCCTCACTCCTAAAGGGGTTTGGGACACTATATCGAGATTCCTATATGATGTGCCACCTGAATTTGTTGACTCTAAGTACTTCTGTGCTGCTGCTAGAAAAAGAGGTTATGTTCACAATCTTCCAATCGAGAATAGAATCCCTCTCGTTCCACTTCCCCCACAAACCATAGATGAGGCATTGCCCATGTCAAGAAAGTGGTGGCCTTCTTGGGACACACGGACAAAGCTAAATTGCTTACAAACTTGCCATTCAAGTGCAAAGTTAACAGAGAGGATCATGAAGGCTCTTGAGGGCTATGATGACATTCCACCAGAGAGAGTCCAGCGGGGTGTAATGCATGAATGCCGTAAGTGGAATCTAGTGTGGGTTGGAAAAAATAGGGTCGCCCCACTTGAGCCTGATGAATTTGAAATGCTTTTGGGGTTTCCAAAGAACCACACCAGGGGAATAAGCAGGACTGATAGATATAAATCACTCGGGAATGCATTTCAGATTGACACCGTCGCATACCACTTCTCTGTTTTGCGAGACATGTTTCCCAATGGCATCAATGTCCTCTCCCTTTTCTCTGGGATAGGTGGGGCGGAAATAGCCCTTCATCGGCTTGGTATCCGTATGAAGAATGTTGTGTCTCTAGAGATTTCAGAAGTTAACAGAAACGTTCTGAGGTGTTGGTGGGAGCAAACAAACCAGAGGGGGAATTTGATTCACCTTCCTGATGTGCAAGAGCTTAATGCTGACCGCTTGGAACAGTTTATGAGTTCATTTGGTGGCTTTGATCTAGTAGTTGGTGGCAGTCCCTGCAACAATCTTACAGGTAGCAATCGATATCATAGAGATGGACTCGAGGGTAAAGAATCTTCCCTATTCTATCATTTCTTTCGTATACTGGACTTGGTTAAGTCTATTATGGCAGGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

556

Amino Acids

62.8

Weight (kDa)

6.11

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 433 - 547 2.9e-08 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 653
AccI GTMKAC 1 cut(s) 1633
AciI CCGC 3 cut(s) 1072, 1331, 1489
AclI AACGTT 1 cut(s) 1407
AclWI GGATC 1 cut(s) 1028
AcsI RAATTY 4 cut(s) 26, 806, 1151, 1447
AfaI GTAC 3 cut(s) 73, 386, 824
AfiI CCNNNNNNNGG 4 cut(s) 195, 653, 1189, 1347
AflIII ACRYGT 1 cut(s) 1278
AgsI TTSAA 5 cut(s) 25, 125, 281, 999, 1157
AhdI GACNNNNNGTC 1 cut(s) 633
AjnI CCWGG 2 cut(s) 517, 1187
Alw21I GWGCWC 1 cut(s) 209
Alw26I GTCTC 4 cut(s) 620, 641, 1269, 1383
AlwI GGATC 1 cut(s) 1028
AlwNI CAGNNNCTG 1 cut(s) 1548
Ama87I CYCGRG 2 cut(s) 1223, 1592
AoxI GGCC 1 cut(s) 947
ApeKI GCWGC 4 cut(s) 467, 707, 833, 836
ApoI RAATTY 4 cut(s) 26, 806, 1151, 1447
Asp700I GAANNNNTTC 1 cut(s) 73
AspS9I GGNCC 2 cut(s) 674, 716
AsuHPI GGTGA 2 cut(s) 329, 1448
AvaI CYCGRG 2 cut(s) 1223, 1592
AvaII GGWCC 2 cut(s) 674, 716
BanII GRGCYC 1 cut(s) 209
BarI GAAGNNNNNNTAC 2 cut(s) 1591, 1623
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 4 cut(s) 454, 719, 820, 823
BccI CCATC 4 cut(s) 59, 250, 268, 1580
BceAI ACGGC 1 cut(s) 1077
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 2 cut(s) 519, 1189
BciVI GTATCC 1 cut(s) 1367
BclI TGATCA 2 cut(s) 241, 372
BcoDI GTCTC 4 cut(s) 620, 641, 1269, 1383
BfaI CTAG 7 cut(s) 18, 156, 506, 840, 1106, 1382, 1529
BfmI CTRYAG 1 cut(s) 411
BfuI GTATCC 1 cut(s) 1367
BisI GCNGC 4 cut(s) 468, 708, 834, 837
BlsI GCNGC 4 cut(s) 469, 709, 835, 838
BmcAI AGTACT 2 cut(s) 73, 824
Bme1390I CCNGG 2 cut(s) 519, 1189
Bme18I GGWCC 2 cut(s) 674, 716
BmeRI GACNNNNNGTC 1 cut(s) 633
BmeT110I CYCGRG 2 cut(s) 1223, 1592
BmgT120I GGNCC 2 cut(s) 674, 716
BmrFI CCNGG 2 cut(s) 519, 1189
BmsI GCATC 2 cut(s) 481, 1302
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 4 cut(s) 191, 223, 1575, 1607
BpuEI CTTGAG 2 cut(s) 1055, 1160
Bsa29I ATCGAT 1 cut(s) 1573
BsaBI GATNNNNATC 1 cut(s) 1216
BsaJI CCNNGG 2 cut(s) 345, 1188
BsaXI ACNNNNNCTCC 2 cut(s) 130, 160
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 653, 1189, 1347
Bse1I ACTGG 3 cut(s) 175, 676, 1641
Bse3DI GCAATG 2 cut(s) 12, 923
Bse8I GATNNNNATC 1 cut(s) 1216
BseBI CCWGG 2 cut(s) 519, 1189
BseCI ATCGAT 1 cut(s) 1573
BseDI CCNNGG 2 cut(s) 345, 1188
BseGI GGATG 3 cut(s) 70, 261, 341
BseJI GATNNNNATC 1 cut(s) 1216
BseLI CCNNNNNNNGG 4 cut(s) 195, 653, 1189, 1347
BseMI GCAATG 2 cut(s) 12, 923
BseMII CTCAG 1 cut(s) 1403
BseNI ACTGG 3 cut(s) 175, 676, 1641
BseRI GAGGAG 2 cut(s) 362, 410
BseXI GCAGC 4 cut(s) 454, 719, 820, 823
BshFI GGCC 1 cut(s) 949
BshVI ATCGAT 1 cut(s) 1573
BsiHKAI GWGCWC 1 cut(s) 209
BsiHKCI CYCGRG 2 cut(s) 1223, 1592
BslFI GGGAC 3 cut(s) 782, 971, 1530
BslI CCNNNNNNNGG 4 cut(s) 195, 653, 1189, 1347
BsmAI GTCTC 4 cut(s) 620, 641, 1269, 1383
BsmBI CGTCTC 1 cut(s) 641
BsmFI GGGAC 3 cut(s) 782, 971, 1530
BsmI GAATGC 2 cut(s) 1094, 1234
BsnI GGCC 1 cut(s) 949
BsoBI CYCGRG 2 cut(s) 1223, 1592
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 4 cut(s) 241, 372, 1020, 1525
BspACI CCGC 3 cut(s) 1072, 1331, 1489
BspANI GGCC 1 cut(s) 949
BspCNI CTCAG 1 cut(s) 1404
BspDI ATCGAT 1 cut(s) 1573
BspHI TCATGA 2 cut(s) 330, 1023
BspPI GGATC 1 cut(s) 1028
BspQI GCTCTTC 1 cut(s) 439
BsrDI GCAATG 2 cut(s) 12, 923
BsrI ACTGG 3 cut(s) 175, 676, 1641
BssECI CCNNGG 2 cut(s) 345, 1188
BssMI GATC 4 cut(s) 241, 372, 1020, 1525
BssNAI GTATAC 1 cut(s) 1634
Bst1107I GTATAC 1 cut(s) 1634
Bst2UI CCWGG 2 cut(s) 519, 1189
Bst4CI ACNGT 3 cut(s) 631, 1249, 1500
Bst6I CTCTTC 3 cut(s) 387, 439, 702
BstAPI GCANNNNNTGC 2 cut(s) 491, 1548
BstC8I GCNNGC 1 cut(s) 205
BstDEI CTNAG 6 cut(s) 44, 267, 428, 549, 819, 1412
BstF5I GGATG 3 cut(s) 70, 261, 341
BstKTI GATC 4 cut(s) 244, 375, 1023, 1528
BstMAI GTCTC 4 cut(s) 620, 641, 1269, 1383
BstMBI GATC 4 cut(s) 241, 372, 1020, 1525
BstMWI GCNNNNNNNGC 6 cut(s) 407, 476, 491, 604, 1337, 1548
BstNI CCWGG 2 cut(s) 519, 1189
BstNSI RCATGY 1 cut(s) 1282
BstSCI CCNGG 2 cut(s) 517, 1187
BstSFI CTRYAG 1 cut(s) 411
BstV1I GCAGC 4 cut(s) 454, 719, 820, 823
BstV2I GAAGAC 1 cut(s) 93
BstZ17I GTATAC 1 cut(s) 1634
Bsu15I ATCGAT 1 cut(s) 1573
BsuI GTATCC 1 cut(s) 1367
BsuRI GGCC 1 cut(s) 949
BsuTUI ATCGAT 1 cut(s) 1573
BtsCI GGATG 3 cut(s) 70, 261, 341
BtsIMutI CAGTG 1 cut(s) 537
Cac8I GCNNGC 1 cut(s) 205
CaiI CAGNNNCTG 1 cut(s) 1548
CciI TCATGA 2 cut(s) 330, 1023
Cfr13I GGNCC 2 cut(s) 674, 716
ClaI ATCGAT 1 cut(s) 1573
Csp6I GTAC 3 cut(s) 72, 385, 823
CviAII CATG 9 cut(s) 210, 299, 331, 364, 678, 931, 1024, 1085, 1279
CviQI GTAC 3 cut(s) 72, 385, 823
DdeI CTNAG 6 cut(s) 44, 267, 428, 549, 819, 1412
DpnI GATC 4 cut(s) 243, 374, 1022, 1527
DpnII GATC 4 cut(s) 241, 372, 1020, 1525
DriI GACNNNNNGTC 1 cut(s) 633
Eam1104I CTCTTC 3 cut(s) 387, 439, 702
Eam1105I GACNNNNNGTC 1 cut(s) 633
EarI CTCTTC 3 cut(s) 387, 439, 702
EciI GGCGGA 1 cut(s) 1346
Ecl136II GAGCTC 1 cut(s) 207
Eco24I GRGCYC 1 cut(s) 209
Eco32I GATATC 1 cut(s) 1577
Eco47I GGWCC 2 cut(s) 674, 716
Eco53kI GAGCTC 1 cut(s) 207
Eco88I CYCGRG 2 cut(s) 1223, 1592
EcoICRI GAGCTC 1 cut(s) 207
EcoO109I RGGNCCY 1 cut(s) 716
EcoRII CCWGG 2 cut(s) 517, 1187
EcoRV GATATC 1 cut(s) 1577
EcoT22I ATGCAT 2 cut(s) 1086, 1234
EcoT38I GRGCYC 1 cut(s) 209
Esp3I CGTCTC 1 cut(s) 641
FaeI CATG 9 cut(s) 213, 302, 334, 367, 681, 934, 1027, 1088, 1282
FalI AAGNNNNNCTT 6 cut(s) 27, 59, 105, 137, 480, 512
FaqI GGGAC 3 cut(s) 782, 971, 1530
FatI CATG 9 cut(s) 209, 298, 330, 363, 677, 930, 1023, 1084, 1278
FauI CCCGC 1 cut(s) 1065
FbaI TGATCA 2 cut(s) 241, 372
FblI GTMKAC 1 cut(s) 1633
Fnu4HI GCNGC 4 cut(s) 468, 708, 834, 837
FokI GGATG 3 cut(s) 77, 268, 328
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 4 cut(s) 468, 708, 834, 837
FspBI CTAG 7 cut(s) 18, 156, 506, 840, 1106, 1382, 1529
GluI GCNGC 4 cut(s) 468, 708, 834, 837
HaeIII GGCC 1 cut(s) 949
Hin1II CATG 9 cut(s) 213, 302, 334, 367, 681, 934, 1027, 1088, 1282
HincII GTYRAC 3 cut(s) 814, 1011, 1399
HindII GTYRAC 3 cut(s) 814, 1011, 1399
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 9 cut(s) 334, 783, 815, 882, 1065, 1102, 1453, 1590, 1604
HpaI GTTAAC 2 cut(s) 1011, 1399
HphI GGTGA 2 cut(s) 329, 1448
Hpy166II GTNNAC 6 cut(s) 387, 814, 859, 1011, 1399, 1634
Hpy188I TCNGA 4 cut(s) 346, 1239, 1393, 1413
Hpy8I GTNNAC 6 cut(s) 387, 814, 859, 1011, 1399, 1634
Hpy99I CGWCG 1 cut(s) 1253
HpyAV CCTTC 6 cut(s) 199, 506, 960, 1021, 1352, 1469
HpyCH4III ACNGT 3 cut(s) 631, 1249, 1500
HpyCH4IV ACGT 1 cut(s) 1407
HpyCH4V TGCA 6 cut(s) 535, 1004, 1084, 1232, 1471, 1551
HpyF10VI GCNNNNNNNGC 6 cut(s) 407, 476, 491, 604, 1337, 1548
HpyF3I CTNAG 6 cut(s) 44, 267, 428, 549, 819, 1412
HpySE526I ACGT 1 cut(s) 1407
Hsp92II CATG 9 cut(s) 213, 302, 334, 367, 681, 934, 1027, 1088, 1282
Ksp22I TGATCA 2 cut(s) 241, 372
KspAI GTTAAC 2 cut(s) 1011, 1399
Kzo9I GATC 4 cut(s) 241, 372, 1020, 1525
LguI GCTCTTC 1 cut(s) 439
LmnI GCTCC 1 cut(s) 1426
Lsp1109I GCAGC 4 cut(s) 454, 719, 820, 823
LweI GCATC 2 cut(s) 481, 1302
MaeI CTAG 7 cut(s) 18, 156, 506, 840, 1106, 1382, 1529
MaeII ACGT 1 cut(s) 1407
MalI GATC 4 cut(s) 243, 374, 1022, 1527
MboI GATC 4 cut(s) 241, 372, 1020, 1525
MhlI GDGCHC 1 cut(s) 209
MluCI AATT 8 cut(s) 26, 125, 288, 446, 806, 976, 1151, 1447
MlyI GAGTC 4 cut(s) 343, 809, 1074, 1584
MmeI TCCRAC 3 cut(s) 249, 692, 1096
Mph1103I ATGCAT 2 cut(s) 1086, 1234
MroXI GAANNNNTTC 1 cut(s) 73
MseI TTAA 5 cut(s) 573, 1010, 1398, 1479, 1647
MslI CAYNNNNRTG 2 cut(s) 999, 1584
MspA1I CMGCKG 1 cut(s) 1072
MspR9I CCNGG 2 cut(s) 519, 1189
Mva1269I GAATGC 2 cut(s) 1094, 1234
MvaI CCWGG 2 cut(s) 519, 1189
MwoI GCNNNNNNNGC 6 cut(s) 407, 476, 491, 604, 1337, 1548
NdeII GATC 4 cut(s) 241, 372, 1020, 1525
NlaIII CATG 9 cut(s) 213, 302, 334, 367, 681, 934, 1027, 1088, 1282
NsiI ATGCAT 2 cut(s) 1086, 1234
NspI RCATGY 1 cut(s) 1282
PaeR7I CTCGAG 1 cut(s) 1592
PagI TCATGA 2 cut(s) 330, 1023
PciI ACATGT 1 cut(s) 1278
PciSI GCTCTTC 1 cut(s) 439
PctI GAATGC 2 cut(s) 1094, 1234
PdmI GAANNNNTTC 1 cut(s) 73
PfeI GAWTC 5 cut(s) 783, 882, 1102, 1453, 1604
PflFI GACNNNGTC 1 cut(s) 1247
PflMI CCANNNNNTGG 1 cut(s) 653
PkrI GCNGC 4 cut(s) 469, 709, 835, 838
PleI GAGTC 4 cut(s) 342, 809, 1073, 1584
PpsI GAGTC 4 cut(s) 342, 809, 1073, 1584
PpuMI RGGWCCY 1 cut(s) 716
PscI ACATGT 1 cut(s) 1278
Psp124BI GAGCTC 1 cut(s) 209
Psp1406I AACGTT 1 cut(s) 1407
Psp5II RGGWCCY 1 cut(s) 716
Psp6I CCWGG 2 cut(s) 517, 1187
PspGI CCWGG 2 cut(s) 517, 1187
PspPI GGNCC 2 cut(s) 674, 716
PspPPI RGGWCCY 1 cut(s) 716
PspXI VCTCGAGB 1 cut(s) 1592
PstNI CAGNNNCTG 1 cut(s) 1548
PsyI GACNNNGTC 1 cut(s) 1247
RsaI GTAC 3 cut(s) 73, 386, 824
RsaNI GTAC 3 cut(s) 72, 385, 823
RseI CAYNNNNRTG 2 cut(s) 999, 1584
SacI GAGCTC 1 cut(s) 209
SapI GCTCTTC 1 cut(s) 439
SaqAI TTAA 5 cut(s) 573, 1010, 1398, 1479, 1647
SatI GCNGC 4 cut(s) 468, 708, 834, 837
Sau3AI GATC 4 cut(s) 241, 372, 1020, 1525
Sau96I GGNCC 2 cut(s) 674, 716
ScaI AGTACT 2 cut(s) 73, 824
SchI GAGTC 4 cut(s) 343, 809, 1074, 1584
ScrFI CCNGG 2 cut(s) 519, 1189
SduI GDGCHC 1 cut(s) 209
SfaNI GCATC 2 cut(s) 481, 1302
SfcI CTRYAG 1 cut(s) 411
Sfr274I CTCGAG 1 cut(s) 1592
SinI GGWCC 2 cut(s) 674, 716
SlaI CTCGAG 1 cut(s) 1592
SmiMI CAYNNNNRTG 2 cut(s) 999, 1584
SmlI CTYRAG 3 cut(s) 1034, 1139, 1592
SmoI CTYRAG 3 cut(s) 1034, 1139, 1592
Sse9I AATT 8 cut(s) 26, 125, 288, 446, 806, 976, 1151, 1447
SsiI CCGC 3 cut(s) 1072, 1331, 1489
SspMI CTAG 7 cut(s) 18, 156, 506, 840, 1106, 1382, 1529
SstI GAGCTC 1 cut(s) 209
StyD4I CCNGG 2 cut(s) 517, 1187
TaaI ACNGT 3 cut(s) 631, 1249, 1500
TaiI ACGT 1 cut(s) 1410
TaqI TCGA 5 cut(s) 584, 779, 873, 1573, 1593
TasI AATT 8 cut(s) 26, 125, 288, 446, 806, 976, 1151, 1447
TatI WGTACW 2 cut(s) 71, 822
TfiI GAWTC 5 cut(s) 783, 882, 1102, 1453, 1604
Tru1I TTAA 5 cut(s) 573, 1010, 1398, 1479, 1647
Tru9I TTAA 5 cut(s) 573, 1010, 1398, 1479, 1647
TscAI CASTG 1 cut(s) 544
TseI GCWGC 4 cut(s) 467, 707, 833, 836
TspDTI ATGAA 6 cut(s) 1040, 1101, 1164, 1334, 1379, 1500
TspGWI ACGGA 2 cut(s) 979, 1349
TspRI CASTG 1 cut(s) 544
Tth111I GACNNNGTC 1 cut(s) 1247
Van91I CCANNNNNTGG 1 cut(s) 653
VpaK11BI GGWCC 2 cut(s) 674, 716
XapI RAATTY 4 cut(s) 26, 806, 1151, 1447
XbaI TCTAGA 2 cut(s) 505, 1381
XceI RCATGY 1 cut(s) 1282
XhoI CTCGAG 1 cut(s) 1592
XmiI GTMKAC 1 cut(s) 1633
XmnI GAANNNNTTC 1 cut(s) 73
XspI CTAG 7 cut(s) 18, 156, 506, 840, 1106, 1382, 1529
ZrmI AGTACT 2 cut(s) 73, 824
Zsp2I ATGCAT 2 cut(s) 1086, 1234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.