Rorug02G0559000

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000338
Physical Location & Seq
Forward (+)
68500298 .. 68502566
2269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0559000.1

Sequence Viewer

Length: 1971 bp
ATGAGACACCCATGGCAGCGTCTGCTTCTTCTCCGAGCTCACCTCCGTTCCTCGCCTCACGTGTTCTCACAGTCTTCTTACTCTCAGGTAAACTCCCAATCGAATCTCCATTCATTTTCGCTCTTCCACACTGACACTCTCCACCCAAAGCTTCCAACTTTACAGCCCATTTTCAATTACCCACTAAACCCTAGAAACCCCATTTCACGAAGCCTCTCGTCGGAGCCAGCACTTGAAACTAAAGACTCCGATCACGGGGTCATAGCTGAAATTTTCTCTAGGCCTAGGGATGAAATTGATATTAGGAAAGACTTGGAATTGAACAATGTAGAGTTTACCCATGACTTTGTTTTGAGTGTTTTGAAGAGCTTAGAATCGAGCCCTGATGTTGCGAGGAGGGTTTTCGATTGGGTTTTGTTATCTGAAGGTAAGAGATTGAGCTCGAAATCGTATAATCAGATGCTGTGTATTTTGGGTGTCAATGGGGTTGTGAAGGAGTTCTGGGAGTTGGTTTATGTTATGAAGAAGAAAGGGTATGGCGTGTCGAAATGGGTTCATGATAGGGTTTTAGAGAAATTTGAGGGGGATGGGTTGGAGAATGATGCCGAGGAATTGAGAGGGGTGTTTGCATCAGGGTCTACTGACAGTTCAGTAGCGAAGGTTTGTTCGACGGTGTGTAAGATTGTTAGAAATGAGGTGTGGAGTGATGATATTGAGAGGCAAATAAAGGAGTTGAATGTTTCATTTTCTAGTGATGTGGTTAAAATGGTGGTGGAGAATCTTAAAACAGAGCCAACAAAAGCACTGATCTTTTTTCGGTGGGTGGAGGAGGCTGGGTTGTTGAAGCATGATCAACAGACGTATAATGCAGTGGCAATGGTATTGGGAAGGACAGACTGTAGGGATAGGTTTTGGAAAGTAGTTGATGACATGAGGAGCAATGGGTGTGAACTGGAGTTGAACACGTATGATAAGGTTTTAGAGAGGTTTTGTAAGAGGGGGGAACTGAAAGATGCTGTGGACTTGTATGAGTTCGCAATGGCTGGTAAAAAAAAGCCTCCGGTGGCTTGTTGTACTTTCCTGTTGAAGAAAATAGTGGTTGGTAAGGAGCTGGACATGGGTTTATTTTCGAGGGTTGTGAGGATTTATACAGAGAGTGGGAATGCCTTGACAAATTCAATGCTCAATGCAGTCCTCAAGTCTTTGATCAGTGTTGGTAGACTTGGAGAGTGCAATAAGGTTTTCAAAGTAATGGAGGAAGGTGGGCTTGTAGCTAGTGCTGGTTTACAGAGTATAATTGCATTCAGGCTTGGTAGTGCTGGTAAAGAGGATGAGGCAAGTGACTTCATTAATATTATGCAAGCGTCTGGCACTGATCTGAATTACAAGGCATGGTCGTCCTTGATTGAGGGATATTGTGTAGCTGGTGATCTTGAGAAGGCCTCAGATTGTTTCCAAAAGATGATTGAAAGGGAGGGGGTTGCCTCTGCAGGTTCTGCTTTTGAATTGGTAGTCAATGCATATTGCAGAAAGAACAGGGCAACTGATGCATACAAGTTTCTCTCTGATTTGGTCAGTAAAAGGCAGCTCAAGCCATGGCAGACCACATACAAACTGTTGATTAGCAAGTTATTGGCTCAGGGTGAATTTAGAAACGCTCTGCATATTCTGGGTCTGATGAAAAATGATCGATTTCCTCCTTTTATTGATCCATTCATTAAGCATGTATCAAAGTCTGGAACTGGTGATGACACTATAGCTTTTCTGAAGATGATGGCTTCAAAGGAATTTCCATCCACCATCGTGTTTCTCCGAGTATTTGAAGCCTATTTTAAGGCTGGACGGCGTAGCGAAGCACAAGATTTCCTTTCAAAATGCCCGGGCTACATTCGTAATCATGCTGATGTACTGAACCTATTCTGTCCTGGAAAATCTGGAGAAGGTGCTGCAACCAAAGTTGTTGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

656

Amino Acids

73.77

Weight (kDa)

8.47

Isoelectric Point (pI)

35.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 464 - 491 9.8e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1481
AccI GTMKAC 2 cut(s) 638, 1219
AclWI GGATC 1 cut(s) 1703
AcsI RAATTY 5 cut(s) 270, 575, 1174, 1646, 1787
AcuI CTGAAG 2 cut(s) 444, 1787
AcvI CACGTG 1 cut(s) 61
AfaI GTAC 2 cut(s) 1075, 1908
AfiI CCNNNNNNNGG 2 cut(s) 220, 255
AflIII ACRYGT 2 cut(s) 60, 963
AjnI CCWGG 1 cut(s) 1924
AleI CACNNNNGTG 1 cut(s) 1802
Alw21I GWGCWC 2 cut(s) 40, 443
AlwI GGATC 1 cut(s) 1703
AlwNI CAGNNNCTG 3 cut(s) 22, 463, 1496
Ama87I CYCGRG 1 cut(s) 1879
AoxI GGCC 2 cut(s) 281, 1440
ApeKI GCWGC 4 cut(s) 16, 1585, 1946, 1964
ApoI RAATTY 5 cut(s) 270, 575, 1174, 1646, 1787
AseI ATTAAT 1 cut(s) 1350
Asp700I GAANNNNTTC 2 cut(s) 497, 1916
AspA2I CCTAGG 1 cut(s) 284
AsuC2I CCSGG 2 cut(s) 1880, 1881
AsuHPI GGTGA 4 cut(s) 32, 1439, 1655, 1757
AvaI CYCGRG 1 cut(s) 1879
AvrII CCTAGG 1 cut(s) 284
BanII GRGCYC 3 cut(s) 40, 383, 443
BbrPI CACGTG 1 cut(s) 61
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 2 cut(s) 40, 443
BbvI GCAGC 4 cut(s) 28, 1597, 1933, 1951
BccI CCATC 4 cut(s) 581, 1768, 1801, 1808
BceAI ACGGC 1 cut(s) 1859
BciT130I CCWGG 1 cut(s) 1926
BclI TGATCA 2 cut(s) 850, 1206
BcnI CCSGG 2 cut(s) 1880, 1881
BfaI CTAG 5 cut(s) 192, 279, 285, 750, 1275
BfmI CTRYAG 3 cut(s) 898, 1488, 1755
BfuAI ACCTGC 1 cut(s) 1481
BisI GCNGC 4 cut(s) 17, 1586, 1947, 1965
BlnI CCTAGG 1 cut(s) 284
BlsI GCNGC 4 cut(s) 18, 1587, 1948, 1966
Bme1390I CCNGG 3 cut(s) 1880, 1881, 1926
BmeT110I CYCGRG 1 cut(s) 1879
BmiI GGNNCC 1 cut(s) 225
BmrFI CCNGG 3 cut(s) 1880, 1881, 1926
BmsI GCATC 5 cut(s) 450, 592, 638, 1003, 1537
BpiI GAAGAC 1 cut(s) 66
BplI GAGNNNNNCTC 4 cut(s) 27, 59, 1427, 1459
BpmI CTGGAG 2 cut(s) 974, 1956
Bpu10I CCTNAGC 1 cut(s) 1638
BpuEI CTTGAG 3 cut(s) 1181, 1454, 1574
BpuMI CCSGG 2 cut(s) 1880, 1881
Bsa29I ATCGAT 1 cut(s) 1690
BsaAI YACGTR 2 cut(s) 61, 966
BsaJI CCNNGG 5 cut(s) 11, 284, 606, 1595, 1879
BsaWI WCCGGW 1 cut(s) 1060
BsaXI ACNNNNNCTCC 2 cut(s) 821, 851
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 255
Bse1I ACTGG 2 cut(s) 957, 1747
Bse3DI GCAATG 3 cut(s) 882, 946, 1044
BseBI CCWGG 1 cut(s) 1926
BseCI ATCGAT 1 cut(s) 1690
BseDI CCNNGG 5 cut(s) 11, 284, 606, 1595, 1879
BseGI GGATG 4 cut(s) 295, 592, 1336, 1793
BseLI CCNNNNNNNGG 2 cut(s) 220, 255
BseMI GCAATG 3 cut(s) 882, 946, 1044
BseMII CTCAG 3 cut(s) 98, 1458, 1652
BseNI ACTGG 2 cut(s) 957, 1747
BseRI GAGGAG 3 cut(s) 409, 842, 949
BseXI GCAGC 4 cut(s) 28, 1597, 1933, 1951
BseYI CCCAGC 1 cut(s) 833
BshFI GGCC 2 cut(s) 283, 1442
BshVI ATCGAT 1 cut(s) 1690
BsiHKAI GWGCWC 2 cut(s) 40, 443
BsiHKCI CYCGRG 1 cut(s) 1879
BsiSI CCGG 2 cut(s) 1061, 1880
BslI CCNNNNNNNGG 2 cut(s) 220, 255
BsmI GAATGC 2 cut(s) 1168, 1301
BsnI GGCC 2 cut(s) 283, 1442
BsoBI CYCGRG 1 cut(s) 1879
Bsp1286I GDGCHC 3 cut(s) 40, 383, 443
Bsp143I GATC 8 cut(s) 250, 807, 850, 1206, 1375, 1429, 1687, 1708
Bsp19I CCATGG 2 cut(s) 11, 1595
BspANI GGCC 2 cut(s) 283, 1442
BspCNI CTCAG 3 cut(s) 97, 1457, 1651
BspDI ATCGAT 1 cut(s) 1690
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 225
BspMAI CTGCAG 1 cut(s) 1492
BspMI ACCTGC 1 cut(s) 1481
BspPI GGATC 1 cut(s) 1703
BspQI GCTCTTC 2 cut(s) 128, 359
BsrDI GCAATG 3 cut(s) 882, 946, 1044
BsrI ACTGG 2 cut(s) 957, 1747
BssECI CCNNGG 5 cut(s) 11, 284, 606, 1595, 1879
BssMI GATC 8 cut(s) 250, 807, 850, 1206, 1375, 1429, 1687, 1708
BssT1I CCWWGG 3 cut(s) 11, 284, 1595
Bst2UI CCWGG 1 cut(s) 1926
Bst4CI ACNGT 5 cut(s) 72, 647, 673, 899, 1617
Bst6I CTCTTC 2 cut(s) 128, 359
BstAPI GCANNNNNTGC 3 cut(s) 22, 1496, 1547
BstBAI YACGTR 2 cut(s) 61, 966
BstC8I GCNNGC 2 cut(s) 228, 1362
BstDEI CTNAG 5 cut(s) 84, 370, 1444, 1638, 1968
BstDSI CCRYGG 2 cut(s) 11, 1595
BstF5I GGATG 4 cut(s) 295, 592, 1336, 1793
BstKTI GATC 8 cut(s) 253, 810, 853, 1209, 1378, 1432, 1690, 1711
BstMBI GATC 8 cut(s) 250, 807, 850, 1206, 1375, 1429, 1687, 1708
BstMWI GCNNNNNNNGC 4 cut(s) 22, 1496, 1547, 1591
BstNI CCWGG 1 cut(s) 1926
BstNSI RCATGY 1 cut(s) 1727
BstSCI CCNGG 3 cut(s) 1878, 1879, 1924
BstSFI CTRYAG 3 cut(s) 898, 1488, 1755
BstV1I GCAGC 4 cut(s) 28, 1597, 1933, 1951
BstV2I GAAGAC 1 cut(s) 66
Bsu15I ATCGAT 1 cut(s) 1690
BsuRI GGCC 2 cut(s) 283, 1442
BsuTUI ATCGAT 1 cut(s) 1690
BtgI CCRYGG 2 cut(s) 11, 1595
BtsCI GGATG 4 cut(s) 295, 592, 1336, 1793
BtsI GCAGTG 1 cut(s) 876
BtsIMutI CAGTG 5 cut(s) 129, 803, 876, 1216, 1371
BveI ACCTGC 1 cut(s) 1481
Cac8I GCNNGC 2 cut(s) 228, 1362
CaiI CAGNNNCTG 3 cut(s) 22, 463, 1496
CciI TCATGA 1 cut(s) 556
Cfr9I CCCGGG 1 cut(s) 1879
ClaI ATCGAT 1 cut(s) 1690
CseI GACGC 2 cut(s) 8, 1353
Csp6I GTAC 2 cut(s) 1074, 1907
CviQI GTAC 2 cut(s) 1074, 1907
DdeI CTNAG 5 cut(s) 84, 370, 1444, 1638, 1968
DpnI GATC 8 cut(s) 252, 809, 852, 1208, 1377, 1431, 1689, 1710
DpnII GATC 8 cut(s) 250, 807, 850, 1206, 1375, 1429, 1687, 1708
Eam1104I CTCTTC 2 cut(s) 128, 359
EarI CTCTTC 2 cut(s) 128, 359
Ecl136II GAGCTC 2 cut(s) 38, 441
Eco130I CCWWGG 3 cut(s) 11, 284, 1595
Eco147I AGGCCT 2 cut(s) 283, 1442
Eco24I GRGCYC 3 cut(s) 40, 383, 443
Eco53kI GAGCTC 2 cut(s) 38, 441
Eco57I CTGAAG 2 cut(s) 444, 1787
Eco72I CACGTG 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 1879
EcoICRI GAGCTC 2 cut(s) 38, 441
EcoRII CCWGG 1 cut(s) 1924
EcoT14I CCWWGG 3 cut(s) 11, 284, 1595
EcoT22I ATGCAT 2 cut(s) 1522, 1552
EcoT38I GRGCYC 3 cut(s) 40, 383, 443
ErhI CCWWGG 3 cut(s) 11, 284, 1595
FalI AAGNNNNNCTT 4 cut(s) 1251, 1283, 1851, 1883
FbaI TGATCA 2 cut(s) 850, 1206
FblI GTMKAC 2 cut(s) 638, 1219
Fnu4HI GCNGC 4 cut(s) 17, 1586, 1947, 1965
FokI GGATG 4 cut(s) 302, 599, 1343, 1780
FriOI GRGCYC 3 cut(s) 40, 383, 443
Fsp4HI GCNGC 4 cut(s) 17, 1586, 1947, 1965
FspBI CTAG 5 cut(s) 192, 279, 285, 750, 1275
GluI GCNGC 4 cut(s) 17, 1586, 1947, 1965
GsaI CCCAGC 1 cut(s) 837
GsuI CTGGAG 2 cut(s) 974, 1956
HaeIII GGCC 2 cut(s) 283, 1442
HapII CCGG 2 cut(s) 1061, 1880
HgaI GACGC 2 cut(s) 8, 1353
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 4 cut(s) 103, 245, 374, 778
HpaII CCGG 2 cut(s) 1061, 1880
HphI GGTGA 4 cut(s) 32, 1439, 1655, 1757
Hpy166II GTNNAC 7 cut(s) 91, 336, 639, 950, 1021, 1220, 1286
Hpy188III TCNNGA 5 cut(s) 207, 557, 1433, 1737, 1935
Hpy8I GTNNAC 7 cut(s) 91, 336, 639, 950, 1021, 1220, 1286
Hpy99I CGWCG 2 cut(s) 223, 673
HpyAV CCTTC 7 cut(s) 419, 487, 652, 882, 1253, 1432, 1934
HpyCH4III ACNGT 5 cut(s) 72, 647, 673, 899, 1617
HpyCH4IV ACGT 3 cut(s) 60, 860, 965
HpyF10VI GCNNNNNNNGC 4 cut(s) 22, 1496, 1547, 1591
HpyF3I CTNAG 5 cut(s) 84, 370, 1444, 1638, 1968
HpySE526I ACGT 3 cut(s) 60, 860, 965
Ksp22I TGATCA 2 cut(s) 850, 1206
Kzo9I GATC 8 cut(s) 250, 807, 850, 1206, 1375, 1429, 1687, 1708
LguI GCTCTTC 2 cut(s) 128, 359
LmnI GCTCC 3 cut(s) 223, 936, 1108
Lsp1109I GCAGC 4 cut(s) 28, 1597, 1933, 1951
LweI GCATC 5 cut(s) 450, 592, 638, 1003, 1537
MaeI CTAG 5 cut(s) 192, 279, 285, 750, 1275
MaeII ACGT 3 cut(s) 60, 860, 965
MaeIII GTNAC 1 cut(s) 1340
MalI GATC 8 cut(s) 252, 809, 852, 1208, 1377, 1431, 1689, 1710
MboI GATC 8 cut(s) 250, 807, 850, 1206, 1375, 1429, 1687, 1708
MboII GAAGA 8 cut(s) 20, 66, 115, 376, 535, 538, 1099, 1780
MhlI GDGCHC 3 cut(s) 40, 383, 443
MlyI GAGTC 1 cut(s) 239
MmeI TCCRAC 3 cut(s) 179, 201, 573
Mph1103I ATGCAT 2 cut(s) 1522, 1552
MroXI GAANNNNTTC 2 cut(s) 497, 1916
MseI TTAA 5 cut(s) 762, 783, 1350, 1719, 1833
MslI CAYNNNNRTG 2 cut(s) 1802, 1902
MspI CCGG 2 cut(s) 1061, 1880
MspR9I CCNGG 3 cut(s) 1880, 1881, 1926
Mva1269I GAATGC 2 cut(s) 1168, 1301
MvaI CCWGG 1 cut(s) 1926
MwoI GCNNNNNNNGC 4 cut(s) 22, 1496, 1547, 1591
NciI CCSGG 2 cut(s) 1880, 1881
NcoI CCATGG 2 cut(s) 11, 1595
NdeII GATC 8 cut(s) 250, 807, 850, 1206, 1375, 1429, 1687, 1708
NlaIV GGNNCC 1 cut(s) 225
NmeAIII GCCGAG 1 cut(s) 631
NmuCI GTSAC 1 cut(s) 1340
NsiI ATGCAT 2 cut(s) 1522, 1552
NspI RCATGY 1 cut(s) 1727
OliI CACNNNNGTG 1 cut(s) 1802
PagI TCATGA 1 cut(s) 556
PceI AGGCCT 2 cut(s) 283, 1442
PciSI GCTCTTC 2 cut(s) 128, 359
PctI GAATGC 2 cut(s) 1168, 1301
PdmI GAANNNNTTC 2 cut(s) 497, 1916
PfeI GAWTC 3 cut(s) 103, 374, 778
PfoI TCCNGGA 1 cut(s) 1924
PkrI GCNGC 4 cut(s) 18, 1587, 1948, 1966
PleI GAGTC 1 cut(s) 239
PmaCI CACGTG 1 cut(s) 61
PmlI CACGTG 1 cut(s) 61
PpsI GAGTC 1 cut(s) 239
Ppu21I YACGTR 2 cut(s) 61, 966
PshBI ATTAAT 1 cut(s) 1350
Psp124BI GAGCTC 2 cut(s) 40, 443
Psp6I CCWGG 1 cut(s) 1924
PspCI CACGTG 1 cut(s) 61
PspFI CCCAGC 1 cut(s) 833
PspGI CCWGG 1 cut(s) 1924
PspN4I GGNNCC 1 cut(s) 225
PstI CTGCAG 1 cut(s) 1492
PstNI CAGNNNCTG 3 cut(s) 22, 463, 1496
RsaI GTAC 2 cut(s) 1075, 1908
RsaNI GTAC 2 cut(s) 1074, 1907
RseI CAYNNNNRTG 2 cut(s) 1802, 1902
SacI GAGCTC 2 cut(s) 40, 443
SapI GCTCTTC 2 cut(s) 128, 359
SaqAI TTAA 5 cut(s) 762, 783, 1350, 1719, 1833
SatI GCNGC 4 cut(s) 17, 1586, 1947, 1965
Sau3AI GATC 8 cut(s) 250, 807, 850, 1206, 1375, 1429, 1687, 1708
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 3 cut(s) 1880, 1881, 1926
SduI GDGCHC 3 cut(s) 40, 383, 443
SfaNI GCATC 5 cut(s) 450, 592, 638, 1003, 1537
SfcI CTRYAG 3 cut(s) 898, 1488, 1755
SmaI CCCGGG 1 cut(s) 1881
SmiMI CAYNNNNRTG 2 cut(s) 1802, 1902
SmlI CTYRAG 3 cut(s) 1196, 1433, 1589
SmoI CTYRAG 3 cut(s) 1196, 1433, 1589
SrfI GCCCGGGC 1 cut(s) 1881
SseBI AGGCCT 2 cut(s) 283, 1442
SspI AATATT 1 cut(s) 1354
SspMI CTAG 5 cut(s) 192, 279, 285, 750, 1275
SstI GAGCTC 2 cut(s) 40, 443
StuI AGGCCT 2 cut(s) 283, 1442
StyD4I CCNGG 3 cut(s) 1878, 1879, 1924
StyI CCWWGG 3 cut(s) 11, 284, 1595
TaaI ACNGT 5 cut(s) 72, 647, 673, 899, 1617
TaiI ACGT 3 cut(s) 63, 863, 968
TaqI TCGA 8 cut(s) 101, 377, 405, 443, 545, 668, 1130, 1690
TatI WGTACW 2 cut(s) 1073, 1906
TfiI GAWTC 3 cut(s) 103, 374, 778
Tru1I TTAA 5 cut(s) 762, 783, 1350, 1719, 1833
Tru9I TTAA 5 cut(s) 762, 783, 1350, 1719, 1833
TscAI CASTG 5 cut(s) 136, 810, 876, 1216, 1378
TseFI GTSAC 1 cut(s) 1340
TseI GCWGC 4 cut(s) 16, 1585, 1946, 1964
Tsp45I GTSAC 1 cut(s) 1340
TspDTI ATGAA 8 cut(s) 102, 306, 536, 545, 732, 1336, 1694, 1705
TspGWI ACGGA 1 cut(s) 35
TspMI CCCGGG 1 cut(s) 1879
TspRI CASTG 5 cut(s) 136, 810, 876, 1216, 1378
VspI ATTAAT 1 cut(s) 1350
XapI RAATTY 5 cut(s) 270, 575, 1174, 1646, 1787
XceI RCATGY 1 cut(s) 1727
XmaI CCCGGG 1 cut(s) 1879
XmaJI CCTAGG 1 cut(s) 284
XmiI GTMKAC 2 cut(s) 638, 1219
XmnI GAANNNNTTC 2 cut(s) 497, 1916
XspI CTAG 5 cut(s) 192, 279, 285, 750, 1275
Zsp2I ATGCAT 2 cut(s) 1522, 1552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.