Rw2G031420

C-5 cytosine-specific DNA methylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000338
Physical Location & Seq
Forward (+)
51516906 .. 51521962
5057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031420.1

Sequence Viewer

Length: 1731 bp
ATGGATGGAAGTAATTCTAGTGTAGATGACAATGATGTTGACTGGAACACAGATGATGAAATCGACAACTTCACTCTCTCGCCTTCTTCGAGTGTGATACATCCTTGTGGAGAAGCTAATGTGCTCTCTTGGGAGGTGAGCTCAGCTCCAACTTCTTCCAATACCAAAGTTCTTGATCAGTTTATAGCGATGGGATTTTCTAAGAAGATGGTTGCCAATGCAATTCAGAAACATGGAGAAGAAAATACAGATGCAATTTTGGAAATGCTCCTCACATATTCGGCTCTAGAAAGCTCAACTCATGAACAGAAACAAATGGATTCTGATCACTTCTCAGATTCTGTGGAAAGCTTTCTAGATGGTTTTTCAGATATGGATAGCTGTGATGATGAGGAAATCATAAGTCATACATCTGAGGAGGAAAAACTAATGTCCTTGATCACAATGGGGTACACAGAAGAGGAGGCTTCCATGGCAGTAGAAAGATGTGGTCATAACTCCTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCAATTGGCAAAGGAAGAAGATGCCCTTCTTCCTCTTGAAGAGAAGCCCAGACTAAAACATGTGCACACTGATCCTAAGAAGAAGAGGAAAATTATTGTATCTGATATATCGAAAAGGAAAAGGCAGATGGGGCACATAGACACAGATACTGAGACACTTTGCCTCCCAAATCCAATGATTGGGTTTGGGATTCCAACTGGAGCATGGAAATCTAACAGAATTCTTCCAAAGGCAGCCATAGGGCCTCCATATTTTTATTATGAGAATGTCGCCCTTACTCCTAAAGGGGTTTGGGACACTATATCCAAATTCCTATATGATGTTCCCCCTGAGTTTGTTGATTCAAAACATTTCTGTTCTGCTGCTAGAAAAAGGGGTTATGTCCATAATCTTCCTACTGAGAACAGATTTCCTCTTGTTCCGCTCCCCCCACAAACCATACATGAGGCATTTCCCTTGACAAAAAAATGGTGGCCTTCTTGGGACATAAGAACAAAGCTAAACTGTATAGTAACTTGCTATTCTAGTGCAAAGTTATCAGAGAGGATAATGAAGCTTCTCGAGGGCTGTGATGGCATTCCACCAGAAAGAGTCCAGCGGGCTGTTATACATGAATGCCGTAAGTGGAATCTATTGTGGGTTGGAAGAAATAGGGTTGCCCCACTTGAGCCTGATGAAGTGGAAATGCTCATGGGGTTTCCAAAGGATCACACAAGGGGAATGAGCCGCACTGATAGATACAAATCACTTGGCAATGCATTTCAGATTCACACTGTGGCATACCACTTCTCAGTCTTGCGAGACAAGTTTCCAAATGGTATAAATGTCCTCTCTCTATTCTCCGGGATAGGTGGAGCAGAAGTAGCCCTCCATCGTCTTGGAATCCATATGAAGAATGTGGTGTCCGTAGAGATATCAGAAGTGAACAGAAACGTTGTGAGGTGTTGGTGGGAGCAAACAAACCAGACGGGAAATTTGATTCACCTTGCTGATGTAAAGGAGCTAGATGCTAACAGATTGGAACAGTTGATGTTTTCATTCGGTGGCTTTGATCTTGTAGTGGGTGGGAGCCCCTGCAATAATCTAACAGGCAGCAATAGATATCATCGAGATGGACTCGAGGGCAAAGAATCTTCACTTTTCTACCACTACTTTCGAATTCTGGACTTGGTCAAGTCTATTATGGCAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

576

Amino Acids

64.96

Weight (kDa)

5.53

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 454 - 568 6.2e-09 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 716
AccBSI CCGCTC 1 cut(s) 961
AciI CCGC 3 cut(s) 959, 1135, 1264
AclI AACGTT 1 cut(s) 1470
AclWI GGATC 2 cut(s) 602, 1251
AcsI RAATTY 4 cut(s) 756, 845, 1510, 1695
AdeI CACNNNGTG 1 cut(s) 1312
AfaI GTAC 1 cut(s) 452
AfiI CCNNNNNNNGG 1 cut(s) 716
AflIII ACRYGT 1 cut(s) 595
AgsI TTSAA 2 cut(s) 575, 882
AluBI AGCT 9 cut(s) 116, 141, 146, 294, 351, 381, 1036, 1093, 1540
AluI AGCT 9 cut(s) 116, 141, 146, 294, 351, 381, 1036, 1093, 1540
Alw21I GWGCWC 3 cut(s) 126, 143, 603
Alw26I GTCTC 2 cut(s) 683, 1332
Alw44I GTGCAC 1 cut(s) 599
AlwI GGATC 2 cut(s) 602, 1251
AlwNI CAGNNNCTG 2 cut(s) 341, 686
Ama87I CYCGRG 2 cut(s) 1097, 1655
AoxI GGCC 2 cut(s) 779, 1010
ApaLI GTGCAC 1 cut(s) 599
ApeKI GCWGC 4 cut(s) 533, 770, 899, 1629
ApoI RAATTY 4 cut(s) 756, 845, 1510, 1695
ArsI GACNNNNNNTTYG 2 cut(s) 1495, 1527
Asp700I GAANNNNTTC 2 cut(s) 13, 351
AspS9I GGNCC 1 cut(s) 779
AsuC2I CCSGG 1 cut(s) 1381
AsuHPI GGTGA 2 cut(s) 148, 1511
AsuII TTCGAA 1 cut(s) 1693
AvaI CYCGRG 2 cut(s) 1097, 1655
BaeGI GKGCMC 2 cut(s) 603, 672
BanII GRGCYC 2 cut(s) 143, 1610
Bbv12I GWGCWC 3 cut(s) 126, 143, 603
BbvI GCAGC 4 cut(s) 520, 782, 886, 1641
BccI CCATC 7 cut(s) 184, 202, 353, 658, 1103, 1416, 1643
BceAI ACGGC 1 cut(s) 1140
BclI TGATCA 3 cut(s) 175, 325, 438
BcnI CCSGG 1 cut(s) 1381
BcoDI GTCTC 2 cut(s) 683, 1332
BfaI CTAG 6 cut(s) 18, 287, 356, 903, 1062, 1541
BisI GCNGC 5 cut(s) 534, 771, 900, 1264, 1630
BlpI GCTNAGC 1 cut(s) 142
BlsI GCNGC 5 cut(s) 535, 772, 901, 1265, 1631
Bme1390I CCNGG 1 cut(s) 1381
BmeT110I CYCGRG 2 cut(s) 1097, 1655
BmgT120I GGNCC 1 cut(s) 779
BmiI GGNNCC 1 cut(s) 1607
BmrFI CCNGG 1 cut(s) 1381
BmsI GCATC 3 cut(s) 241, 547, 1534
BplI GAGNNNNNCTC 6 cut(s) 125, 157, 130, 162, 1638, 1670
BpmI CTGGAG 1 cut(s) 756
Bpu1102I GCTNAGC 1 cut(s) 142
Bpu14I TTCGAA 1 cut(s) 1693
BpuEI CTTGAG 1 cut(s) 1223
BpuMI CCSGG 1 cut(s) 1381
BsaBI GATNNNNATC 2 cut(s) 324, 1279
BsaJI CCNNGG 1 cut(s) 471
BsaXI ACNNNNNCTCC 4 cut(s) 684, 714, 1383, 1413
Bsc4I CCNNNNNNNGG 1 cut(s) 716
Bse1I ACTGG 2 cut(s) 47, 739
Bse3DI GCAATG 1 cut(s) 1297
Bse8I GATNNNNATC 2 cut(s) 324, 1279
BseDI CCNNGG 1 cut(s) 471
BseGI GGATG 2 cut(s) 10, 100
BseJI GATNNNNATC 2 cut(s) 324, 1279
BseLI CCNNNNNNNGG 1 cut(s) 716
BseMI GCAATG 1 cut(s) 1297
BseMII CTCAG 7 cut(s) 156, 348, 405, 678, 858, 927, 1341
BseNI ACTGG 2 cut(s) 47, 739
BseRI GAGGAG 4 cut(s) 260, 431, 476, 490
BseSI GKGCMC 2 cut(s) 603, 672
BseXI GCAGC 4 cut(s) 520, 782, 886, 1641
BshFI GGCC 2 cut(s) 781, 1012
BsiHKAI GWGCWC 3 cut(s) 126, 143, 603
BsiHKCI CYCGRG 2 cut(s) 1097, 1655
BsiSI CCGG 1 cut(s) 1380
BslFI GGGAC 2 cut(s) 845, 1034
BslI CCNNNNNNNGG 1 cut(s) 716
BsmAI GTCTC 2 cut(s) 683, 1332
BsmFI GGGAC 2 cut(s) 845, 1034
BsmI GAATGC 2 cut(s) 1113, 1157
BsnI GGCC 2 cut(s) 781, 1012
BsoBI CYCGRG 2 cut(s) 1097, 1655
Bsp119I TTCGAA 1 cut(s) 1693
Bsp1286I GDGCHC 5 cut(s) 126, 143, 603, 672, 1610
Bsp143I GATC 6 cut(s) 175, 325, 438, 607, 1243, 1588
Bsp1720I GCTNAGC 1 cut(s) 142
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 3 cut(s) 959, 1135, 1264
BspANI GGCC 2 cut(s) 781, 1012
BspCNI CTCAG 7 cut(s) 155, 347, 406, 679, 859, 928, 1340
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 1607
BspPI GGATC 2 cut(s) 602, 1251
BspT104I TTCGAA 1 cut(s) 1693
BsrBI CCGCTC 1 cut(s) 961
BsrDI GCAATG 1 cut(s) 1297
BsrI ACTGG 2 cut(s) 47, 739
BssECI CCNNGG 1 cut(s) 471
BssMI GATC 6 cut(s) 175, 325, 438, 607, 1243, 1588
BssT1I CCWWGG 1 cut(s) 471
Bst4CI ACNGT 3 cut(s) 1043, 1312, 1563
Bst6I CTCTTC 3 cut(s) 453, 570, 614
BstBI TTCGAA 1 cut(s) 1693
BstC8I GCNNGC 1 cut(s) 1137
BstDEI CTNAG 9 cut(s) 142, 201, 334, 414, 612, 687, 867, 936, 1327
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 2 cut(s) 10, 100
BstKTI GATC 6 cut(s) 178, 328, 441, 610, 1246, 1591
BstMAI GTCTC 2 cut(s) 683, 1332
BstMBI GATC 6 cut(s) 175, 325, 438, 607, 1243, 1588
BstMWI GCNNNNNNNGC 5 cut(s) 473, 542, 667, 1110, 1400
BstNSI RCATGY 1 cut(s) 599
BstSCI CCNGG 1 cut(s) 1379
BstSLI GKGCMC 2 cut(s) 603, 672
BstV1I GCAGC 4 cut(s) 520, 782, 886, 1641
BstXI CCANNNNNNTGG 1 cut(s) 1415
BsuRI GGCC 2 cut(s) 781, 1012
BtgI CCRYGG 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 203
BtsCI GGATG 2 cut(s) 10, 100
BtsIMutI CAGTG 3 cut(s) 603, 1266, 1308
Cac8I GCNNGC 1 cut(s) 1137
CaiI CAGNNNCTG 2 cut(s) 341, 686
CciI TCATGA 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 779
Csp6I GTAC 1 cut(s) 451
CviAII CATG 8 cut(s) 233, 302, 472, 596, 741, 980, 1148, 1228
CviQI GTAC 1 cut(s) 451
DdeI CTNAG 9 cut(s) 142, 201, 334, 414, 612, 687, 867, 936, 1327
DpnI GATC 6 cut(s) 177, 327, 440, 609, 1245, 1590
DpnII GATC 6 cut(s) 175, 325, 438, 607, 1243, 1588
DraIII CACNNNGTG 1 cut(s) 1312
Eam1104I CTCTTC 3 cut(s) 453, 570, 614
EarI CTCTTC 3 cut(s) 453, 570, 614
Ecl136II GAGCTC 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 2 cut(s) 143, 1610
Eco32I GATATC 2 cut(s) 1452, 1640
Eco53kI GAGCTC 1 cut(s) 141
Eco88I CYCGRG 2 cut(s) 1097, 1655
EcoICRI GAGCTC 1 cut(s) 141
EcoO109I RGGNCCY 1 cut(s) 779
EcoRI GAATTC 2 cut(s) 756, 1695
EcoRV GATATC 2 cut(s) 1452, 1640
EcoT14I CCWWGG 1 cut(s) 471
EcoT22I ATGCAT 1 cut(s) 1297
EcoT38I GRGCYC 2 cut(s) 143, 1610
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 8 cut(s) 236, 305, 475, 599, 744, 983, 1151, 1231
FalI AAGNNNNNCTT 2 cut(s) 546, 578
FaqI GGGAC 2 cut(s) 845, 1034
FatI CATG 8 cut(s) 232, 301, 471, 595, 740, 979, 1147, 1227
FauI CCCGC 1 cut(s) 1128
FauNDI CATATG 1 cut(s) 1425
FbaI TGATCA 3 cut(s) 175, 325, 438
Fnu4HI GCNGC 5 cut(s) 534, 771, 900, 1264, 1630
FokI GGATG 2 cut(s) 17, 87
FriOI GRGCYC 2 cut(s) 143, 1610
Fsp4HI GCNGC 5 cut(s) 534, 771, 900, 1264, 1630
FspBI CTAG 6 cut(s) 18, 287, 356, 903, 1062, 1541
GluI GCNGC 5 cut(s) 534, 771, 900, 1264, 1630
GsuI CTGGAG 1 cut(s) 756
HaeIII GGCC 2 cut(s) 781, 1012
HapII CCGG 1 cut(s) 1380
Hin1II CATG 8 cut(s) 236, 305, 475, 599, 744, 983, 1151, 1231
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 2 cut(s) 349, 1091
HpaII CCGG 1 cut(s) 1380
HphI GGTGA 2 cut(s) 148, 1511
Hpy166II GTNNAC 4 cut(s) 40, 453, 601, 1462
Hpy188I TCNGA 9 cut(s) 228, 325, 337, 370, 415, 640, 1078, 1302, 1456
Hpy188III TCNNGA 9 cut(s) 173, 287, 302, 356, 509, 572, 1097, 1646, 1700
Hpy8I GTNNAC 4 cut(s) 40, 453, 601, 1462
HpyAV CCTTC 3 cut(s) 93, 572, 1023
HpyCH4III ACNGT 3 cut(s) 1043, 1312, 1563
HpyCH4IV ACGT 1 cut(s) 1470
HpyCH4V TGCA 6 cut(s) 221, 254, 601, 1067, 1295, 1614
HpyF10VI GCNNNNNNNGC 5 cut(s) 473, 542, 667, 1110, 1400
HpyF3I CTNAG 9 cut(s) 142, 201, 334, 414, 612, 687, 867, 936, 1327
HpySE526I ACGT 1 cut(s) 1470
Hsp92II CATG 8 cut(s) 236, 305, 475, 599, 744, 983, 1151, 1231
Ksp22I TGATCA 3 cut(s) 175, 325, 438
Kzo9I GATC 6 cut(s) 175, 325, 438, 607, 1243, 1588
LmnI GCTCC 8 cut(s) 151, 273, 737, 966, 1391, 1489, 1537, 1605
Lsp1109I GCAGC 4 cut(s) 520, 782, 886, 1641
LweI GCATC 3 cut(s) 241, 547, 1534
MaeI CTAG 6 cut(s) 18, 287, 356, 903, 1062, 1541
MaeII ACGT 1 cut(s) 1470
MaeIII GTNAC 1 cut(s) 1048
MalI GATC 6 cut(s) 177, 327, 440, 609, 1245, 1590
MbiI CCGCTC 1 cut(s) 961
MboI GATC 6 cut(s) 175, 325, 438, 607, 1243, 1588
MfeI CAATTG 1 cut(s) 539
MhlI GDGCHC 5 cut(s) 126, 143, 603, 672, 1610
MlyI GAGTC 2 cut(s) 1137, 1647
MmeI TCCRAC 3 cut(s) 173, 755, 1159
Mph1103I ATGCAT 1 cut(s) 1297
MroXI GAANNNNTTC 2 cut(s) 13, 351
MslI CAYNNNNRTG 2 cut(s) 105, 1647
MspA1I CMGCKG 1 cut(s) 1135
MspI CCGG 1 cut(s) 1380
MspR9I CCNGG 1 cut(s) 1381
MunI CAATTG 1 cut(s) 539
Mva1269I GAATGC 2 cut(s) 1113, 1157
MwoI GCNNNNNNNGC 5 cut(s) 473, 542, 667, 1110, 1400
NciI CCSGG 1 cut(s) 1381
NcoI CCATGG 1 cut(s) 471
NdeI CATATG 1 cut(s) 1425
NdeII GATC 6 cut(s) 175, 325, 438, 607, 1243, 1588
NlaIII CATG 8 cut(s) 236, 305, 475, 599, 744, 983, 1151, 1231
NlaIV GGNNCC 1 cut(s) 1607
NsiI ATGCAT 1 cut(s) 1297
NspI RCATGY 1 cut(s) 599
NspV TTCGAA 1 cut(s) 1693
PaeR7I CTCGAG 2 cut(s) 1097, 1655
PagI TCATGA 1 cut(s) 301
PciI ACATGT 1 cut(s) 595
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PctI GAATGC 2 cut(s) 1113, 1157
PdmI GAANNNNTTC 2 cut(s) 13, 351
PfeI GAWTC 9 cut(s) 320, 338, 727, 878, 1165, 1303, 1419, 1516, 1667
PflFI GACNNNGTC 1 cut(s) 1706
PflMI CCANNNNNTGG 1 cut(s) 716
PfoI TCCNGGA 1 cut(s) 1379
PkrI GCNGC 5 cut(s) 535, 772, 901, 1265, 1631
PleI GAGTC 2 cut(s) 1136, 1647
PpsI GAGTC 2 cut(s) 1136, 1647
PscI ACATGT 1 cut(s) 595
Psp124BI GAGCTC 1 cut(s) 143
Psp1406I AACGTT 1 cut(s) 1470
PspN4I GGNNCC 1 cut(s) 1607
PspPI GGNCC 1 cut(s) 779
PspXI VCTCGAGB 1 cut(s) 1655
PstNI CAGNNNCTG 2 cut(s) 341, 686
PsyI GACNNNGTC 1 cut(s) 1706
RsaI GTAC 1 cut(s) 452
RsaNI GTAC 1 cut(s) 451
RseI CAYNNNNRTG 2 cut(s) 105, 1647
SacI GAGCTC 1 cut(s) 143
SatI GCNGC 5 cut(s) 534, 771, 900, 1264, 1630
Sau3AI GATC 6 cut(s) 175, 325, 438, 607, 1243, 1588
Sau96I GGNCC 1 cut(s) 779
SchI GAGTC 2 cut(s) 1137, 1647
ScrFI CCNGG 1 cut(s) 1381
SduI GDGCHC 5 cut(s) 126, 143, 603, 672, 1610
SfaNI GCATC 3 cut(s) 241, 547, 1534
Sfr274I CTCGAG 2 cut(s) 1097, 1655
SfuI TTCGAA 1 cut(s) 1693
SlaI CTCGAG 2 cut(s) 1097, 1655
SmiMI CAYNNNNRTG 2 cut(s) 105, 1647
SmlI CTYRAG 3 cut(s) 1097, 1202, 1655
SmoI CTYRAG 3 cut(s) 1097, 1202, 1655
SsiI CCGC 3 cut(s) 959, 1135, 1264
SspMI CTAG 6 cut(s) 18, 287, 356, 903, 1062, 1541
SstI GAGCTC 1 cut(s) 143
StyD4I CCNGG 1 cut(s) 1379
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 3 cut(s) 1043, 1312, 1563
TaiI ACGT 1 cut(s) 1473
TaqI TCGA 7 cut(s) 63, 89, 647, 1098, 1645, 1656, 1693
TauI GCSGC 1 cut(s) 1266
TfiI GAWTC 9 cut(s) 320, 338, 727, 878, 1165, 1303, 1419, 1516, 1667
TscAI CASTG 3 cut(s) 610, 1273, 1315
TseI GCWGC 4 cut(s) 533, 770, 899, 1629
TspDTI ATGAA 7 cut(s) 72, 318, 1103, 1164, 1227, 1442, 1563
TspGWI ACGGA 1 cut(s) 1432
TspRI CASTG 3 cut(s) 610, 1273, 1315
Tth111I GACNNNGTC 1 cut(s) 1706
Van91I CCANNNNNTGG 1 cut(s) 716
VneI GTGCAC 1 cut(s) 599
XapI RAATTY 4 cut(s) 756, 845, 1510, 1695
XbaI TCTAGA 2 cut(s) 286, 355
XceI RCATGY 1 cut(s) 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 738
XhoI CTCGAG 2 cut(s) 1097, 1655
XmnI GAANNNNTTC 2 cut(s) 13, 351
XspI CTAG 6 cut(s) 18, 287, 356, 903, 1062, 1541
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.