Rmu_sc0002132.1_g000049

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002132.1
OG0000338
Physical Location & Seq
Forward (+)
197027 .. 199616
2590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002132.1_g000049.1.cds

Sequence Viewer

Length: 1137 bp
atgcctacacatctgcagagtatgtatcctgagaagaataaaagggaactttgtgaattttcattgctgaaaaggaaaaggattgtgggacatggaaaccagatcataggggaagatgctggtgcaattcatctcccaaatccaatgaatgggtttgggactccagctgatctctgtcaaatccacagaagtcttccagaggcagccacaaaacctccttatttttactatgagaatgtggcattgactcctaaaggagtttggagcactatatcccgcttgttgtatgacgtgaagcctgagtatgttgattcgaagtatttttgtgctgctgcaagaaaaaggggttatgttcaaaatctttcaatcaaaaacagaattcctcccgttccctttccaccacaaactatatttgatgcatttcccatgacaaggaggtggtggcctacttgggatgattggacaaagctaaattgcttacagacttgcattgctagtgcaacaattactgagaggatcatgaaggctcttaaagactatgacgaagatgaaatcccatcagaaagtgtccgtcgctatgttctttatgaatgctgcaagtggaatcttgtctgggtaggaaagaaaaaggtggccccacttgagcctgatgaagttgaaatgcttttggggttcccaagggaccacacaaggggaataagcaggacagttagatataagtcaatgggaaattcattccagatcgacacagtggcttatcacctgttagttttgaaagatcgatttcccaatggcatcaatgtcctctctcttttctctgggattgttggtgcagagatagctcttcatcgtcttggtatcctgatgaagaatgttgtgtcagtggagatatcagagttgagcagaaatgttgtgaggtgttggtgggagcagacaaaccagagagggaacttgtatcaccttgctgatgtgcaagagttgaacgctaaccgcttagagcattatatcatgttcctttgctcatgtatcggacttttggagtctcaatttgagatttcttctttctcagatcctagctcggaaactgggacttatggtggcttcctggcagttggccctagtgttgagaacttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

378

Amino Acids

43.22

Weight (kDa)

7.97

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 149
AciI CCGC 2 cut(s) 277, 991
AclWI GGATC 2 cut(s) 524, 1064
AcsI RAATTY 3 cut(s) 56, 378, 730
AfiI CCNNNNNNNGG 3 cut(s) 149, 432, 691
AgsI TTSAA 5 cut(s) 356, 366, 659, 775, 982
AjiI CACGTC 1 cut(s) 292
AjnI CCWGG 1 cut(s) 1104
AluBI AGCT 4 cut(s) 167, 469, 842, 1077
AluI AGCT 4 cut(s) 167, 469, 842, 1077
Alw21I GWGCWC 1 cut(s) 269
Alw26I GTCTC 1 cut(s) 1047
AlwI GGATC 2 cut(s) 524, 1064
AoxI GGCC 3 cut(s) 443, 633, 1114
ApeKI GCWGC 4 cut(s) 203, 329, 332, 594
ApoI RAATTY 3 cut(s) 56, 378, 730
AspS9I GGNCC 3 cut(s) 634, 682, 1115
AsuHPI GGTGA 2 cut(s) 752, 950
AsuII TTCGAA 1 cut(s) 314
AvaII GGWCC 1 cut(s) 682
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 269
BbvI GCAGC 4 cut(s) 215, 316, 319, 581
BccI CCATC 1 cut(s) 565
BciT130I CCWGG 1 cut(s) 1106
BciVI GTATCC 2 cut(s) 36, 869
BcoDI GTCTC 1 cut(s) 1047
BfaI CTAG 3 cut(s) 495, 1074, 1119
BfmI CTRYAG 1 cut(s) 14
BfuI GTATCC 2 cut(s) 36, 869
BisI GCNGC 4 cut(s) 204, 330, 333, 595
BlsI GCNGC 4 cut(s) 205, 331, 334, 596
Bme1390I CCNGG 1 cut(s) 1106
Bme18I GGWCC 1 cut(s) 682
BmgBI CACGTC 1 cut(s) 292
BmgT120I GGNCC 3 cut(s) 634, 682, 1115
BmiI GGNNCC 3 cut(s) 636, 674, 683
BmrFI CCNGG 1 cut(s) 1106
BmrI ACTGGG 1 cut(s) 1095
BmsI GCATC 3 cut(s) 106, 406, 804
BmuI ACTGGG 1 cut(s) 1095
BpiI GAAGAC 1 cut(s) 185
BpmI CTGGAG 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 314
BpuEI CTTGAG 1 cut(s) 662
Bsa29I ATCGAT 1 cut(s) 781
BsaJI CCNNGG 1 cut(s) 677
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 432, 691
Bse1I ACTGG 1 cut(s) 1090
Bse3DI GCAATG 2 cut(s) 62, 489
BseBI CCWGG 1 cut(s) 1106
BseCI ATCGAT 1 cut(s) 781
BseDI CCNNGG 1 cut(s) 677
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 3 cut(s) 149, 432, 691
BseMI GCAATG 2 cut(s) 62, 489
BseMII CTCAG 4 cut(s) 21, 291, 501, 1080
BseNI ACTGG 1 cut(s) 1090
BseXI GCAGC 4 cut(s) 215, 316, 319, 581
BsgI GTGCAG 1 cut(s) 852
BshFI GGCC 3 cut(s) 445, 635, 1116
BshVI ATCGAT 1 cut(s) 781
BsiHKAI GWGCWC 1 cut(s) 269
BslFI GGGAC 4 cut(s) 102, 172, 695, 1102
BslI CCNNNNNNNGG 3 cut(s) 149, 432, 691
BsmAI GTCTC 1 cut(s) 1047
BsmFI GGGAC 4 cut(s) 102, 172, 695, 1102
BsmI GAATGC 1 cut(s) 596
BsnI GGCC 3 cut(s) 445, 635, 1116
Bsp119I TTCGAA 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 269
Bsp143I GATC 6 cut(s) 102, 169, 516, 741, 778, 1069
BspACI CCGC 2 cut(s) 277, 991
BspANI GGCC 3 cut(s) 445, 635, 1116
BspCNI CTCAG 4 cut(s) 22, 292, 502, 1079
BspDI ATCGAT 1 cut(s) 781
BspHI TCATGA 1 cut(s) 519
BspLI GGNNCC 3 cut(s) 636, 674, 683
BspMAI CTGCAG 1 cut(s) 18
BspPI GGATC 2 cut(s) 524, 1064
BspQI GCTCTTC 1 cut(s) 849
BspT104I TTCGAA 1 cut(s) 314
BsrDI GCAATG 2 cut(s) 62, 489
BsrI ACTGG 1 cut(s) 1090
BssECI CCNNGG 1 cut(s) 677
BssMI GATC 6 cut(s) 102, 169, 516, 741, 778, 1069
BssT1I CCWWGG 1 cut(s) 677
Bst2UI CCWGG 1 cut(s) 1106
Bst4CI ACNGT 2 cut(s) 709, 751
Bst6I CTCTTC 1 cut(s) 849
BstBI TTCGAA 1 cut(s) 314
BstDEI CTNAG 5 cut(s) 30, 300, 510, 994, 1066
BstF5I GGATG 1 cut(s) 460
BstKTI GATC 6 cut(s) 105, 172, 519, 744, 781, 1072
BstMAI GTCTC 1 cut(s) 1047
BstMBI GATC 6 cut(s) 102, 169, 516, 741, 778, 1069
BstMWI GCNNNNNNNGC 1 cut(s) 839
BstNI CCWGG 1 cut(s) 1106
BstSCI CCNGG 1 cut(s) 1104
BstSFI CTRYAG 1 cut(s) 14
BstV1I GCAGC 4 cut(s) 215, 316, 319, 581
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 1 cut(s) 1069
BstYI RGATCY 1 cut(s) 1069
Bsu15I ATCGAT 1 cut(s) 781
BsuI GTATCC 2 cut(s) 36, 869
BsuRI GGCC 3 cut(s) 445, 635, 1116
BsuTUI ATCGAT 1 cut(s) 781
BtrI CACGTC 1 cut(s) 292
BtsCI GGATG 1 cut(s) 460
BtsIMutI CAGTG 2 cut(s) 756, 888
CciI TCATGA 1 cut(s) 519
Cfr13I GGNCC 3 cut(s) 634, 682, 1115
ClaI ATCGAT 1 cut(s) 781
CviAII CATG 5 cut(s) 92, 427, 520, 1009, 1023
DdeI CTNAG 5 cut(s) 30, 300, 510, 994, 1066
DpnI GATC 6 cut(s) 104, 171, 518, 743, 780, 1071
DpnII GATC 6 cut(s) 102, 169, 516, 741, 778, 1069
Eam1104I CTCTTC 1 cut(s) 849
EarI CTCTTC 1 cut(s) 849
Eco130I CCWWGG 1 cut(s) 677
Eco32I GATATC 1 cut(s) 891
Eco47I GGWCC 1 cut(s) 682
EcoRI GAATTC 1 cut(s) 378
EcoRII CCWGG 1 cut(s) 1104
EcoRV GATATC 1 cut(s) 891
EcoT14I CCWWGG 1 cut(s) 677
EcoT22I ATGCAT 1 cut(s) 421
ErhI CCWWGG 1 cut(s) 677
FaeI CATG 5 cut(s) 95, 430, 523, 1012, 1026
FaqI GGGAC 4 cut(s) 102, 172, 695, 1102
FatI CATG 5 cut(s) 91, 426, 519, 1008, 1022
FauI CCCGC 1 cut(s) 284
Fnu4HI GCNGC 4 cut(s) 204, 330, 333, 595
FokI GGATG 1 cut(s) 467
Fsp4HI GCNGC 4 cut(s) 204, 330, 333, 595
FspBI CTAG 3 cut(s) 495, 1074, 1119
GluI GCNGC 4 cut(s) 204, 330, 333, 595
GsuI CTGGAG 1 cut(s) 147
HaeIII GGCC 3 cut(s) 445, 635, 1116
Hin1II CATG 5 cut(s) 95, 430, 523, 1012, 1026
HinfI GANTC 5 cut(s) 160, 247, 311, 604, 1040
HphI GGTGA 2 cut(s) 752, 950
Hpy188I TCNGA 5 cut(s) 562, 895, 1031, 1069, 1081
Hpy188III TCNNGA 5 cut(s) 29, 197, 520, 739, 862
Hpy99I CGWCG 1 cut(s) 576
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 2 cut(s) 709, 751
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 9 cut(s) 16, 125, 335, 419, 489, 500, 597, 833, 973
HpyF10VI GCNNNNNNNGC 1 cut(s) 839
HpyF3I CTNAG 5 cut(s) 30, 300, 510, 994, 1066
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 5 cut(s) 95, 430, 523, 1012, 1026
Kzo9I GATC 6 cut(s) 102, 169, 516, 741, 778, 1069
LguI GCTCTTC 1 cut(s) 849
LmnI GCTCC 2 cut(s) 264, 928
Lsp1109I GCAGC 4 cut(s) 215, 316, 319, 581
LweI GCATC 3 cut(s) 106, 406, 804
MaeI CTAG 3 cut(s) 495, 1074, 1119
MaeII ACGT 1 cut(s) 291
MalI GATC 6 cut(s) 104, 171, 518, 743, 780, 1071
MboI GATC 6 cut(s) 102, 169, 516, 741, 778, 1069
MboII GAAGA 7 cut(s) 46, 125, 185, 557, 836, 880, 1050
MflI RGATCY 1 cut(s) 1069
MhlI GDGCHC 1 cut(s) 269
MluCI AATT 7 cut(s) 56, 126, 378, 472, 504, 730, 1046
MlyI GAGTC 3 cut(s) 154, 241, 1049
MnlI CCTC 8 cut(s) 193, 225, 393, 429, 507, 815, 909, 938
Mph1103I ATGCAT 1 cut(s) 421
MseI TTAA 1 cut(s) 531
MspA1I CMGCKG 1 cut(s) 167
MspR9I CCNGG 1 cut(s) 1106
Mva1269I GAATGC 1 cut(s) 596
MvaI CCWGG 1 cut(s) 1106
MwoI GCNNNNNNNGC 1 cut(s) 839
NdeII GATC 6 cut(s) 102, 169, 516, 741, 778, 1069
NlaIII CATG 5 cut(s) 95, 430, 523, 1012, 1026
NlaIV GGNNCC 3 cut(s) 636, 674, 683
NsiI ATGCAT 1 cut(s) 421
NspV TTCGAA 1 cut(s) 314
PagI TCATGA 1 cut(s) 519
PciSI GCTCTTC 1 cut(s) 849
PctI GAATGC 1 cut(s) 596
PfeI GAWTC 2 cut(s) 311, 604
PflMI CCANNNNNTGG 1 cut(s) 149
PkrI GCNGC 4 cut(s) 205, 331, 334, 596
PleI GAGTC 3 cut(s) 154, 241, 1048
PpsI GAGTC 3 cut(s) 154, 241, 1048
Psp6I CCWGG 1 cut(s) 1104
PspGI CCWGG 1 cut(s) 1104
PspN4I GGNNCC 3 cut(s) 636, 674, 683
PspPI GGNCC 3 cut(s) 634, 682, 1115
PstI CTGCAG 1 cut(s) 18
PsuI RGATCY 1 cut(s) 1069
PvuII CAGCTG 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 849
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 4 cut(s) 204, 330, 333, 595
Sau3AI GATC 6 cut(s) 102, 169, 516, 741, 778, 1069
Sau96I GGNCC 3 cut(s) 634, 682, 1115
SchI GAGTC 3 cut(s) 154, 241, 1049
ScrFI CCNGG 1 cut(s) 1106
SduI GDGCHC 1 cut(s) 269
SfaNI GCATC 3 cut(s) 106, 406, 804
SfcI CTRYAG 1 cut(s) 14
SfuI TTCGAA 1 cut(s) 314
SinI GGWCC 1 cut(s) 682
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 7 cut(s) 56, 126, 378, 472, 504, 730, 1046
SsiI CCGC 2 cut(s) 277, 991
SspMI CTAG 3 cut(s) 495, 1074, 1119
StyD4I CCNGG 1 cut(s) 1104
StyI CCWWGG 1 cut(s) 677
TaaI ACNGT 2 cut(s) 709, 751
TaiI ACGT 1 cut(s) 294
TaqI TCGA 3 cut(s) 314, 744, 781
TasI AATT 7 cut(s) 56, 126, 378, 472, 504, 730, 1046
TfiI GAWTC 2 cut(s) 311, 604
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 2 cut(s) 756, 888
TseI GCWGC 4 cut(s) 203, 329, 332, 594
TspGWI ACGGA 1 cut(s) 560
TspRI CASTG 2 cut(s) 756, 888
Van91I CCANNNNNTGG 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 682
XapI RAATTY 3 cut(s) 56, 378, 730
XspI CTAG 3 cut(s) 495, 1074, 1119
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.