Rh2BG390900

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000338
Physical Location & Seq
Forward (+)
55480639 .. 55483963
3325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG390900.1

Sequence Viewer

Length: 1479 bp
ATGCTCCTCTCAGATTCAGTTCTAGAAAGTTCTTCCCAAGAACTGCAACAAACCGATTCAGATCACTTTTCCTCTGATTATGATGGAAGCTTTTTGGATGACTTTTCAGATTTGGAAAGCTCTGGTGATGAGGAAATCATGAGTCCTACATCCGAGGAGGAAAAGCTACTCATGTCCTTGATCAAAATGGGGTACACAGAAGAGGAGGCTTCCATAGCTATAGAAAGATGTGGTCTTAGCTCTTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCATTTGGCAAAGGCAGAAGATGCCCTTCTTCCTCTAGAAGAGAAGCCTGGACTAAAACATTTGCACACTGATTATCCTAAGAAGAAAAGGAAACTTTTGGTTGATAAAGTTTCGAAAAGGAAAAGGCAAAATAGGCTCGTAGGCGAGGATAATGAGACAGTTCGTCTCCCAAATCCAATGATTGGGTTTGGTATTCCAACTGGACCATGCCAATCTTACAGATATCTTCCAACGGCAGCCATAGGACCTCCCTATTTTTATTATGAGAATGTAGCCCTCACTCCTAAAGGGGTTTGGGACACTATATCGAGATTCCTATATGATGTGCCACCTGAATTTGTTGACTCTAAGTACTTCTGTGCTGCTGCTAGAAAAAGAGGTTATGTTCACAATCTTCCAATCGAGAATAGAATCCCTCTCGTTCCACTTCCCCCACAAACCATACATGAGGCATTGCCCATGTCAAGAAAGTGGTGGCCTTCTTGGGACACACGGACAAAGCTAAATTGCTTACAAACTTGCCATTCAAGTGCAAAGTTAACAGAGAGGATCATGAAGGCTCTTGAGGGCTATGATGACATTCCACCAGAGAGAGTCCAGCGGGGTGTAATGCATGAATGCCGTAAGTGGAATCTAGTGTGGGTTGGAAAAAATAGGGTCGCCCCACTTGAGCCTGATGAATTTGAAATGCTTTTGGGGTTTCCAAAGGACCACACCAGGGGAATAAGCAGGACTGATAGATATAAATCACTCGGGAATGCATTTCAGATTGACACCGTCGCATACCACTTCTCTGTTTTGCGGGACATGTTTCCCAATGGCATCAATGTCCTCTCCCTTTTCTCTGGGATAGGTGGGGCAGAAATAGCCCTTCATCGGCTTGGTATCCGTATGAAGAATGTTGTGTCTCTAGAGATTTCAGAAGTTAACAGAAACGTTCTGAGGTGTTGGTGGGAGCAAACAAACCAGAGGGGGAATTTGATTCACCTTCCTGATGTGCAAGAGCTTAATGCTGACCGCTTGGAACAGTTTATGAGTTCATTTGGTGGCTTTGATCTAGTAGTTGGTGGCAGTCCCTGCAACAATCTTACAGGTAGCAATCGATATCATAGAGATGGACTCGAGGGTAAAGAATCTTCCCTATTCTATCATTTCTTTCGTATACTGGACTTGGTTAAGTCTATTATGGCAGGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

492

Amino Acids

55.92

Weight (kDa)

5.84

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 369 - 483 2.3e-08 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 461
AccI GTMKAC 1 cut(s) 1441
AciI CCGC 3 cut(s) 880, 1081, 1297
AclI AACGTT 1 cut(s) 1215
AclWI GGATC 1 cut(s) 836
AcsI RAATTY 3 cut(s) 614, 959, 1255
AfaI GTAC 2 cut(s) 194, 632
AfiI CCNNNNNNNGG 3 cut(s) 461, 997, 1155
AflIII ACRYGT 1 cut(s) 1086
AgsI TTSAA 2 cut(s) 807, 965
AhdI GACNNNNNGTC 1 cut(s) 441
AjnI CCWGG 2 cut(s) 325, 995
AluBI AGCT 7 cut(s) 90, 120, 166, 218, 240, 781, 1285
AluI AGCT 7 cut(s) 90, 120, 166, 218, 240, 781, 1285
Alw26I GTCTC 3 cut(s) 428, 449, 1191
AlwI GGATC 1 cut(s) 836
AlwNI CAGNNNCTG 1 cut(s) 1356
Ama87I CYCGRG 2 cut(s) 1031, 1400
AoxI GGCC 1 cut(s) 755
ApeKI GCWGC 4 cut(s) 275, 515, 641, 644
ApoI RAATTY 3 cut(s) 614, 959, 1255
AspS9I GGNCC 3 cut(s) 482, 524, 988
AsuHPI GGTGA 2 cut(s) 137, 1256
AsuII TTCGAA 1 cut(s) 392
AvaI CYCGRG 2 cut(s) 1031, 1400
AvaII GGWCC 3 cut(s) 482, 524, 988
BarI GAAGNNNNNNTAC 2 cut(s) 1399, 1431
BbvI GCAGC 4 cut(s) 262, 527, 628, 631
BccI CCATC 2 cut(s) 77, 1388
BceAI ACGGC 2 cut(s) 528, 885
BciT130I CCWGG 2 cut(s) 327, 997
BciVI GTATCC 1 cut(s) 1175
BclI TGATCA 1 cut(s) 180
BcoDI GTCTC 3 cut(s) 428, 449, 1191
BfaI CTAG 6 cut(s) 23, 314, 648, 914, 1190, 1337
BfmI CTRYAG 1 cut(s) 219
BfuI GTATCC 1 cut(s) 1175
BisI GCNGC 4 cut(s) 276, 516, 642, 645
BlsI GCNGC 4 cut(s) 277, 517, 643, 646
BmcAI AGTACT 1 cut(s) 632
Bme1390I CCNGG 2 cut(s) 327, 997
Bme18I GGWCC 3 cut(s) 482, 524, 988
BmeRI GACNNNNNGTC 1 cut(s) 441
BmeT110I CYCGRG 2 cut(s) 1031, 1400
BmgT120I GGNCC 3 cut(s) 482, 524, 988
BmrFI CCNGG 2 cut(s) 327, 997
BmsI GCATC 2 cut(s) 289, 1110
BplI GAGNNNNNCTC 2 cut(s) 1383, 1415
Bpu14I TTCGAA 1 cut(s) 392
BpuEI CTTGAG 2 cut(s) 863, 968
Bsa29I ATCGAT 1 cut(s) 1381
BsaBI GATNNNNATC 2 cut(s) 60, 1024
BsaJI CCNNGG 2 cut(s) 153, 996
Bsc4I CCNNNNNNNGG 3 cut(s) 461, 997, 1155
Bse1I ACTGG 2 cut(s) 484, 1449
Bse3DI GCAATG 1 cut(s) 731
Bse8I GATNNNNATC 2 cut(s) 60, 1024
BseBI CCWGG 2 cut(s) 327, 997
BseCI ATCGAT 1 cut(s) 1381
BseDI CCNNGG 2 cut(s) 153, 996
BseGI GGATG 2 cut(s) 103, 149
BseJI GATNNNNATC 2 cut(s) 60, 1024
BseLI CCNNNNNNNGG 3 cut(s) 461, 997, 1155
BseMI GCAATG 1 cut(s) 731
BseMII CTCAG 2 cut(s) 24, 1211
BseNI ACTGG 2 cut(s) 484, 1449
BseRI GAGGAG 2 cut(s) 170, 218
BseXI GCAGC 4 cut(s) 262, 527, 628, 631
BshFI GGCC 1 cut(s) 757
BshVI ATCGAT 1 cut(s) 1381
BsiHKCI CYCGRG 2 cut(s) 1031, 1400
BslFI GGGAC 4 cut(s) 590, 779, 1097, 1338
BslI CCNNNNNNNGG 3 cut(s) 461, 997, 1155
BsmAI GTCTC 3 cut(s) 428, 449, 1191
BsmBI CGTCTC 1 cut(s) 449
BsmFI GGGAC 4 cut(s) 590, 779, 1097, 1338
BsmI GAATGC 2 cut(s) 902, 1042
BsnI GGCC 1 cut(s) 757
BsoBI CYCGRG 2 cut(s) 1031, 1400
Bsp119I TTCGAA 1 cut(s) 392
Bsp143I GATC 4 cut(s) 61, 180, 828, 1333
BspACI CCGC 3 cut(s) 880, 1081, 1297
BspANI GGCC 1 cut(s) 757
BspCNI CTCAG 2 cut(s) 23, 1212
BspDI ATCGAT 1 cut(s) 1381
BspHI TCATGA 2 cut(s) 138, 831
BspPI GGATC 1 cut(s) 836
BspQI GCTCTTC 1 cut(s) 247
BspT104I TTCGAA 1 cut(s) 392
BsrDI GCAATG 1 cut(s) 731
BsrI ACTGG 2 cut(s) 484, 1449
BssECI CCNNGG 2 cut(s) 153, 996
BssMI GATC 4 cut(s) 61, 180, 828, 1333
BssNAI GTATAC 1 cut(s) 1442
Bst1107I GTATAC 1 cut(s) 1442
Bst2UI CCWGG 2 cut(s) 327, 997
Bst4CI ACNGT 3 cut(s) 439, 1057, 1308
Bst6I CTCTTC 3 cut(s) 195, 247, 312
BstAPI GCANNNNNTGC 2 cut(s) 299, 1356
BstBI TTCGAA 1 cut(s) 392
BstDEI CTNAG 5 cut(s) 10, 236, 357, 627, 1220
BstF5I GGATG 2 cut(s) 103, 149
BstKTI GATC 4 cut(s) 64, 183, 831, 1336
BstMAI GTCTC 3 cut(s) 428, 449, 1191
BstMBI GATC 4 cut(s) 61, 180, 828, 1333
BstMWI GCNNNNNNNGC 6 cut(s) 215, 284, 299, 412, 1145, 1356
BstNI CCWGG 2 cut(s) 327, 997
BstNSI RCATGY 1 cut(s) 1090
BstSCI CCNGG 2 cut(s) 325, 995
BstSFI CTRYAG 1 cut(s) 219
BstV1I GCAGC 4 cut(s) 262, 527, 628, 631
BstZ17I GTATAC 1 cut(s) 1442
Bsu15I ATCGAT 1 cut(s) 1381
BsuI GTATCC 1 cut(s) 1175
BsuRI GGCC 1 cut(s) 757
BsuTUI ATCGAT 1 cut(s) 1381
BtsCI GGATG 2 cut(s) 103, 149
BtsIMutI CAGTG 1 cut(s) 345
CaiI CAGNNNCTG 1 cut(s) 1356
CciI TCATGA 2 cut(s) 138, 831
Cfr13I GGNCC 3 cut(s) 482, 524, 988
ClaI ATCGAT 1 cut(s) 1381
Csp6I GTAC 2 cut(s) 193, 631
CviAII CATG 8 cut(s) 139, 172, 486, 725, 739, 832, 893, 1087
CviQI GTAC 2 cut(s) 193, 631
DdeI CTNAG 5 cut(s) 10, 236, 357, 627, 1220
DpnI GATC 4 cut(s) 63, 182, 830, 1335
DpnII GATC 4 cut(s) 61, 180, 828, 1333
DriI GACNNNNNGTC 1 cut(s) 441
Eam1104I CTCTTC 3 cut(s) 195, 247, 312
Eam1105I GACNNNNNGTC 1 cut(s) 441
EarI CTCTTC 3 cut(s) 195, 247, 312
Eco32I GATATC 2 cut(s) 503, 1385
Eco47I GGWCC 3 cut(s) 482, 524, 988
Eco88I CYCGRG 2 cut(s) 1031, 1400
EcoO109I RGGNCCY 1 cut(s) 524
EcoRII CCWGG 2 cut(s) 325, 995
EcoRV GATATC 2 cut(s) 503, 1385
EcoT22I ATGCAT 2 cut(s) 894, 1042
Esp3I CGTCTC 1 cut(s) 449
FaeI CATG 8 cut(s) 142, 175, 489, 728, 742, 835, 896, 1090
FalI AAGNNNNNCTT 2 cut(s) 288, 320
FaqI GGGAC 4 cut(s) 590, 779, 1097, 1338
FatI CATG 8 cut(s) 138, 171, 485, 724, 738, 831, 892, 1086
FauI CCCGC 2 cut(s) 873, 1074
FbaI TGATCA 1 cut(s) 180
FblI GTMKAC 1 cut(s) 1441
Fnu4HI GCNGC 4 cut(s) 276, 516, 642, 645
FokI GGATG 2 cut(s) 110, 136
Fsp4HI GCNGC 4 cut(s) 276, 516, 642, 645
FspBI CTAG 6 cut(s) 23, 314, 648, 914, 1190, 1337
GluI GCNGC 4 cut(s) 276, 516, 642, 645
HaeIII GGCC 1 cut(s) 757
Hin1II CATG 8 cut(s) 142, 175, 489, 728, 742, 835, 896, 1090
HincII GTYRAC 3 cut(s) 622, 819, 1207
HindII GTYRAC 3 cut(s) 622, 819, 1207
HindIII AAGCTT 1 cut(s) 88
HpaI GTTAAC 2 cut(s) 819, 1207
HphI GGTGA 2 cut(s) 137, 1256
Hpy166II GTNNAC 6 cut(s) 195, 622, 667, 819, 1207, 1442
Hpy188I TCNGA 8 cut(s) 13, 61, 76, 109, 154, 1047, 1201, 1221
Hpy8I GTNNAC 6 cut(s) 195, 622, 667, 819, 1207, 1442
Hpy99I CGWCG 1 cut(s) 1061
HpyAV CCTTC 5 cut(s) 314, 768, 829, 1160, 1277
HpyCH4III ACNGT 3 cut(s) 439, 1057, 1308
HpyCH4IV ACGT 1 cut(s) 1215
HpyCH4V TGCA 7 cut(s) 46, 343, 812, 892, 1040, 1279, 1359
HpyF10VI GCNNNNNNNGC 6 cut(s) 215, 284, 299, 412, 1145, 1356
HpyF3I CTNAG 5 cut(s) 10, 236, 357, 627, 1220
HpySE526I ACGT 1 cut(s) 1215
Hsp92II CATG 8 cut(s) 142, 175, 489, 728, 742, 835, 896, 1090
Ksp22I TGATCA 1 cut(s) 180
KspAI GTTAAC 2 cut(s) 819, 1207
Kzo9I GATC 4 cut(s) 61, 180, 828, 1333
LguI GCTCTTC 1 cut(s) 247
LmnI GCTCC 2 cut(s) 9, 1234
Lsp1109I GCAGC 4 cut(s) 262, 527, 628, 631
LweI GCATC 2 cut(s) 289, 1110
MaeI CTAG 6 cut(s) 23, 314, 648, 914, 1190, 1337
MaeII ACGT 1 cut(s) 1215
MalI GATC 4 cut(s) 63, 182, 830, 1335
MboI GATC 4 cut(s) 61, 180, 828, 1333
MluCI AATT 5 cut(s) 254, 614, 784, 959, 1255
MlyI GAGTC 4 cut(s) 151, 617, 882, 1392
MmeI TCCRAC 3 cut(s) 500, 533, 904
Mph1103I ATGCAT 2 cut(s) 894, 1042
MseI TTAA 4 cut(s) 818, 1206, 1287, 1455
MslI CAYNNNNRTG 2 cut(s) 807, 1392
MspA1I CMGCKG 1 cut(s) 880
MspR9I CCNGG 2 cut(s) 327, 997
Mva1269I GAATGC 2 cut(s) 902, 1042
MvaI CCWGG 2 cut(s) 327, 997
MwoI GCNNNNNNNGC 6 cut(s) 215, 284, 299, 412, 1145, 1356
NdeII GATC 4 cut(s) 61, 180, 828, 1333
NlaIII CATG 8 cut(s) 142, 175, 489, 728, 742, 835, 896, 1090
NsiI ATGCAT 2 cut(s) 894, 1042
NspI RCATGY 1 cut(s) 1090
NspV TTCGAA 1 cut(s) 392
PaeR7I CTCGAG 1 cut(s) 1400
PagI TCATGA 2 cut(s) 138, 831
PciI ACATGT 1 cut(s) 1086
PciSI GCTCTTC 1 cut(s) 247
PctI GAATGC 2 cut(s) 902, 1042
PfeI GAWTC 7 cut(s) 14, 56, 591, 690, 910, 1261, 1412
PflFI GACNNNGTC 1 cut(s) 1055
PflMI CCANNNNNTGG 1 cut(s) 461
PkrI GCNGC 4 cut(s) 277, 517, 643, 646
PleI GAGTC 4 cut(s) 150, 617, 881, 1392
PpsI GAGTC 4 cut(s) 150, 617, 881, 1392
PpuMI RGGWCCY 1 cut(s) 524
PscI ACATGT 1 cut(s) 1086
Psp1406I AACGTT 1 cut(s) 1215
Psp5II RGGWCCY 1 cut(s) 524
Psp6I CCWGG 2 cut(s) 325, 995
PspGI CCWGG 2 cut(s) 325, 995
PspPI GGNCC 3 cut(s) 482, 524, 988
PspPPI RGGWCCY 1 cut(s) 524
PspXI VCTCGAGB 1 cut(s) 1400
PstNI CAGNNNCTG 1 cut(s) 1356
PsyI GACNNNGTC 1 cut(s) 1055
RsaI GTAC 2 cut(s) 194, 632
RsaNI GTAC 2 cut(s) 193, 631
RseI CAYNNNNRTG 2 cut(s) 807, 1392
SapI GCTCTTC 1 cut(s) 247
SaqAI TTAA 4 cut(s) 818, 1206, 1287, 1455
SatI GCNGC 4 cut(s) 276, 516, 642, 645
Sau3AI GATC 4 cut(s) 61, 180, 828, 1333
Sau96I GGNCC 3 cut(s) 482, 524, 988
ScaI AGTACT 1 cut(s) 632
SchI GAGTC 4 cut(s) 151, 617, 882, 1392
ScrFI CCNGG 2 cut(s) 327, 997
SfaNI GCATC 2 cut(s) 289, 1110
SfcI CTRYAG 1 cut(s) 219
Sfr274I CTCGAG 1 cut(s) 1400
SfuI TTCGAA 1 cut(s) 392
SinI GGWCC 3 cut(s) 482, 524, 988
SlaI CTCGAG 1 cut(s) 1400
SmiMI CAYNNNNRTG 2 cut(s) 807, 1392
SmlI CTYRAG 3 cut(s) 842, 947, 1400
SmoI CTYRAG 3 cut(s) 842, 947, 1400
Sse9I AATT 5 cut(s) 254, 614, 784, 959, 1255
SsiI CCGC 3 cut(s) 880, 1081, 1297
SspMI CTAG 6 cut(s) 23, 314, 648, 914, 1190, 1337
StyD4I CCNGG 2 cut(s) 325, 995
TaaI ACNGT 3 cut(s) 439, 1057, 1308
TaiI ACGT 1 cut(s) 1218
TaqI TCGA 5 cut(s) 392, 587, 681, 1381, 1401
TasI AATT 5 cut(s) 254, 614, 784, 959, 1255
TatI WGTACW 1 cut(s) 630
TfiI GAWTC 7 cut(s) 14, 56, 591, 690, 910, 1261, 1412
Tru1I TTAA 4 cut(s) 818, 1206, 1287, 1455
Tru9I TTAA 4 cut(s) 818, 1206, 1287, 1455
TscAI CASTG 1 cut(s) 352
TseI GCWGC 4 cut(s) 275, 515, 641, 644
TspDTI ATGAA 6 cut(s) 848, 909, 972, 1142, 1187, 1308
TspGWI ACGGA 2 cut(s) 787, 1157
TspRI CASTG 1 cut(s) 352
Tth111I GACNNNGTC 1 cut(s) 1055
Van91I CCANNNNNTGG 1 cut(s) 461
VpaK11BI GGWCC 3 cut(s) 482, 524, 988
XapI RAATTY 3 cut(s) 614, 959, 1255
XbaI TCTAGA 3 cut(s) 22, 313, 1189
XceI RCATGY 1 cut(s) 1090
XhoI CTCGAG 1 cut(s) 1400
XmiI GTMKAC 1 cut(s) 1441
XspI CTAG 6 cut(s) 23, 314, 648, 914, 1190, 1337
ZrmI AGTACT 1 cut(s) 632
Zsp2I ATGCAT 2 cut(s) 894, 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.