RchiOBHm_Chr1g0322481

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000338
Physical Location & Seq
Reverse (-)
9844051 .. 9846605
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55247

Sequence Viewer

Length: 951 bp
ATGCTAATTTTGCTCTTCTTTCCAACAAAAAGGAAAAGGATTATGGGACATGGAAACCAGATCATAGGGGAAGATGCTGATGCAATTCATCTCCCAAATCCAATGATTGGGTTCAGGACTCCAGCTGATCTCTGTCAAATCCACAGAAGTCTTCCAGACGCAGTCACAGGACCTCCTTATTGTTACTATGAGAATGTGGCATTGACTCCTAAAGGAGTCTGGGGCACAATATCCCGCTTTTTGTATGACAAAAAGGGCAATGTTCACAATCTTCCAATCAAAAACAGATTTCCTCTCATTCCGCTTCCACCACAAACTATAGTTGATGCATTTCCCATGACAAGGAGGTGGTGGCCTACTTGGGATGATCGGACAAAGCTAAATTGCTTACAGACTTGCACTGCTGGTGCAACAATTACTGATAGGATCATGATGGCTCTTAAAGACTATGACGAAGATGAAATGCCACCTGAAAGTGTCCATCGCTGTGTTCTTTATGAATGCTGCAAGTGGAATCTTGTTTGGATTGATAGTGGCTTATCACCTGTCAGTTTTGAAAGATCGATTTCCCAATGGCATCAATGTCCTCTCTCTCTCTTCTCTGGGTTTGGTGGTGCAGAGATAGCTCTTTACCGGCTTGGTATCCCGATGAAGAATGTTGTGTCAGTGGAGATTTCAGAGGTGAGCAGAAATGTTGTGAGGTGTTGGTGGGAGCAGACAAATCAGGGAGGGAACATGTATCACCTTGCTGATGTGCAAGAGTTGAATGTTGACTTCTTAGAGCCTTATATCAGTTCCTTTGGCGGGTTTGATCTGGTGAGTGGTGGCAGTCCATGTAACAATCTTGCGGGTAGCAACAGACACCATCGGGATGGGCTTAAGGATGTCAGCTCGGAAACGGGGACTTATGGTGACTTCCTGACAGTTGGCCCTAGCGTTGAGAACTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

316

Amino Acids

35.59

Weight (kDa)

5.85

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 107
AciI CCGC 4 cut(s) 235, 302, 804, 848
AclWI GGATC 1 cut(s) 434
AfiI CCNNNNNNNGG 3 cut(s) 107, 342, 804
AflII CTTAAG 1 cut(s) 878
AflIII ACRYGT 1 cut(s) 735
AgsI TTSAA 2 cut(s) 557, 766
AluBI AGCT 4 cut(s) 125, 379, 626, 891
AluI AGCT 4 cut(s) 125, 379, 626, 891
AlwI GGATC 1 cut(s) 434
AoxI GGCC 2 cut(s) 353, 928
ApeKI GCWGC 1 cut(s) 504
AspS9I GGNCC 2 cut(s) 170, 929
AsuHPI GGTGA 5 cut(s) 534, 694, 734, 829, 923
AvaII GGWCC 1 cut(s) 170
BaeGI GKGCMC 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 143
BbvI GCAGC 1 cut(s) 491
BccI CCATC 4 cut(s) 427, 489, 866, 873
BciVI GTATCC 1 cut(s) 653
BfaI CTAG 1 cut(s) 933
BfmI CTRYAG 1 cut(s) 318
BfrI CTTAAG 1 cut(s) 878
BfuI GTATCC 1 cut(s) 653
BisI GCNGC 1 cut(s) 505
BlsI GCNGC 1 cut(s) 506
Bme18I GGWCC 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 170, 929
BmsI GCATC 4 cut(s) 64, 70, 316, 586
BpiI GAAGAC 1 cut(s) 143
BpmI CTGGAG 1 cut(s) 105
Bsa29I ATCGAT 1 cut(s) 563
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 3 cut(s) 107, 342, 804
Bse118I RCCGGY 1 cut(s) 633
Bse3DI GCAATG 1 cut(s) 265
BseCI ATCGAT 1 cut(s) 563
BseGI GGATG 3 cut(s) 370, 877, 889
BseLI CCNNNNNNNGG 3 cut(s) 107, 342, 804
BseMI GCAATG 1 cut(s) 265
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 1 cut(s) 491
BsgI GTGCAG 1 cut(s) 636
BshFI GGCC 2 cut(s) 355, 930
BshVI ATCGAT 1 cut(s) 563
BsiSI CCGG 1 cut(s) 634
BslFI GGGAC 2 cut(s) 60, 916
BslI CCNNNNNNNGG 3 cut(s) 107, 342, 804
BsmFI GGGAC 2 cut(s) 60, 916
BsmI GAATGC 1 cut(s) 506
BsnI GGCC 2 cut(s) 355, 930
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 6 cut(s) 60, 127, 367, 426, 560, 811
BspACI CCGC 4 cut(s) 235, 302, 804, 848
BspANI GGCC 2 cut(s) 355, 930
BspDI ATCGAT 1 cut(s) 563
BspHI TCATGA 1 cut(s) 429
BspPI GGATC 1 cut(s) 434
BspQI GCTCTTC 1 cut(s) 20
BspTI CTTAAG 1 cut(s) 878
BsrDI GCAATG 1 cut(s) 265
BsrFI RCCGGY 1 cut(s) 633
BssAI RCCGGY 1 cut(s) 633
BssMI GATC 6 cut(s) 60, 127, 367, 426, 560, 811
Bst4CI ACNGT 1 cut(s) 925
Bst6I CTCTTC 2 cut(s) 20, 602
BstAFI CTTAAG 1 cut(s) 878
BstDEI CTNAG 1 cut(s) 778
BstF5I GGATG 3 cut(s) 370, 877, 889
BstKTI GATC 6 cut(s) 63, 130, 370, 429, 563, 814
BstMBI GATC 6 cut(s) 60, 127, 367, 426, 560, 811
BstMWI GCNNNNNNNGC 2 cut(s) 10, 623
BstNSI RCATGY 1 cut(s) 739
BstSFI CTRYAG 1 cut(s) 318
BstSLI GKGCMC 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 491
BstV2I GAAGAC 1 cut(s) 143
BstXI CCANNNNNNTGG 1 cut(s) 872
Bsu15I ATCGAT 1 cut(s) 563
BsuI GTATCC 1 cut(s) 653
BsuRI GGCC 2 cut(s) 355, 930
BsuTUI ATCGAT 1 cut(s) 563
BtgZI GCGATG 1 cut(s) 467
BtsCI GGATG 3 cut(s) 370, 877, 889
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 2 cut(s) 399, 672
CciI TCATGA 1 cut(s) 429
Cfr10I RCCGGY 1 cut(s) 633
Cfr13I GGNCC 2 cut(s) 170, 929
ClaI ATCGAT 1 cut(s) 563
CseI GACGC 1 cut(s) 167
CviAII CATG 5 cut(s) 50, 337, 430, 736, 834
DdeI CTNAG 1 cut(s) 778
DpnI GATC 6 cut(s) 62, 129, 369, 428, 562, 813
DpnII GATC 6 cut(s) 60, 127, 367, 426, 560, 811
Eam1104I CTCTTC 2 cut(s) 20, 602
EarI CTCTTC 2 cut(s) 20, 602
Eco47I GGWCC 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 170
EcoT22I ATGCAT 1 cut(s) 331
FaeI CATG 5 cut(s) 53, 340, 433, 739, 837
FaqI GGGAC 2 cut(s) 60, 916
FatI CATG 5 cut(s) 49, 336, 429, 735, 833
FauI CCCGC 3 cut(s) 242, 797, 841
Fnu4HI GCNGC 1 cut(s) 505
FokI GGATG 3 cut(s) 377, 884, 896
Fsp4HI GCNGC 1 cut(s) 505
FspBI CTAG 1 cut(s) 933
GluI GCNGC 1 cut(s) 505
GsuI CTGGAG 1 cut(s) 105
HaeIII GGCC 2 cut(s) 355, 930
HapII CCGG 1 cut(s) 634
HgaI GACGC 1 cut(s) 167
Hin1II CATG 5 cut(s) 53, 340, 433, 739, 837
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 4 cut(s) 118, 205, 216, 514
HpaII CCGG 1 cut(s) 634
HphI GGTGA 5 cut(s) 534, 694, 734, 829, 923
Hpy166II GTNNAC 2 cut(s) 265, 772
Hpy188I TCNGA 3 cut(s) 372, 679, 895
Hpy188III TCNNGA 6 cut(s) 115, 155, 430, 646, 869, 919
Hpy8I GTNNAC 2 cut(s) 265, 772
HpyCH4III ACNGT 1 cut(s) 925
HpyCH4V TGCA 7 cut(s) 83, 329, 399, 410, 507, 617, 757
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 623
HpyF3I CTNAG 1 cut(s) 778
Hsp92II CATG 5 cut(s) 53, 340, 433, 739, 837
Kzo9I GATC 6 cut(s) 60, 127, 367, 426, 560, 811
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 1 cut(s) 712
Lsp1109I GCAGC 1 cut(s) 491
LweI GCATC 4 cut(s) 64, 70, 316, 586
MaeI CTAG 1 cut(s) 933
MaeIII GTNAC 4 cut(s) 163, 182, 836, 911
MalI GATC 6 cut(s) 62, 129, 369, 428, 562, 813
MboI GATC 6 cut(s) 60, 127, 367, 426, 560, 811
MboII GAAGA 7 cut(s) 7, 83, 143, 263, 467, 589, 664
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 4 cut(s) 6, 84, 382, 414
MlyI GAGTC 3 cut(s) 112, 199, 225
MmeI TCCRAC 1 cut(s) 47
MnlI CCTC 7 cut(s) 183, 303, 339, 597, 673, 693, 722
Mph1103I ATGCAT 1 cut(s) 331
MseI TTAA 2 cut(s) 441, 879
MslI CAYNNNNRTG 2 cut(s) 486, 870
MspA1I CMGCKG 1 cut(s) 125
MspCI CTTAAG 1 cut(s) 878
MspI CCGG 1 cut(s) 634
Mva1269I GAATGC 1 cut(s) 506
MwoI GCNNNNNNNGC 2 cut(s) 10, 623
NdeII GATC 6 cut(s) 60, 127, 367, 426, 560, 811
NlaIII CATG 5 cut(s) 53, 340, 433, 739, 837
NmuCI GTSAC 2 cut(s) 163, 911
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 1 cut(s) 739
PagI TCATGA 1 cut(s) 429
PciI ACATGT 1 cut(s) 735
PciSI GCTCTTC 1 cut(s) 20
PctI GAATGC 1 cut(s) 506
PfeI GAWTC 1 cut(s) 514
PflFI GACNNNGTC 1 cut(s) 161
PflMI CCANNNNNTGG 1 cut(s) 107
PkrI GCNGC 1 cut(s) 506
PleI GAGTC 3 cut(s) 112, 199, 224
PpsI GAGTC 3 cut(s) 112, 199, 224
PpuMI RGGWCCY 1 cut(s) 170
PscI ACATGT 1 cut(s) 735
Psp5II RGGWCCY 1 cut(s) 170
PspPI GGNCC 2 cut(s) 170, 929
PspPPI RGGWCCY 1 cut(s) 170
PsyI GACNNNGTC 1 cut(s) 161
PvuII CAGCTG 1 cut(s) 125
RseI CAYNNNNRTG 2 cut(s) 486, 870
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 2 cut(s) 441, 879
SatI GCNGC 1 cut(s) 505
Sau3AI GATC 6 cut(s) 60, 127, 367, 426, 560, 811
Sau96I GGNCC 2 cut(s) 170, 929
SchI GAGTC 3 cut(s) 112, 199, 225
SduI GDGCHC 1 cut(s) 227
SfaNI GCATC 4 cut(s) 64, 70, 316, 586
SfcI CTRYAG 1 cut(s) 318
SinI GGWCC 1 cut(s) 170
SmiMI CAYNNNNRTG 2 cut(s) 486, 870
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
Sse9I AATT 4 cut(s) 6, 84, 382, 414
SsiI CCGC 4 cut(s) 235, 302, 804, 848
SspMI CTAG 1 cut(s) 933
TaaI ACNGT 1 cut(s) 925
TaqI TCGA 1 cut(s) 563
TasI AATT 4 cut(s) 6, 84, 382, 414
TfiI GAWTC 1 cut(s) 514
Tru1I TTAA 2 cut(s) 441, 879
Tru9I TTAA 2 cut(s) 441, 879
TscAI CASTG 2 cut(s) 406, 672
TseFI GTSAC 2 cut(s) 163, 911
TseI GCWGC 1 cut(s) 504
Tsp45I GTSAC 2 cut(s) 163, 911
TspDTI ATGAA 4 cut(s) 77, 474, 513, 665
TspRI CASTG 2 cut(s) 406, 672
Tth111I GACNNNGTC 1 cut(s) 161
Van91I CCANNNNNTGG 1 cut(s) 107
Vha464I CTTAAG 1 cut(s) 878
VpaK11BI GGWCC 1 cut(s) 170
XceI RCATGY 1 cut(s) 739
XspI CTAG 1 cut(s) 933
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.