RLG00000019575

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000338
Physical Location & Seq
Forward (+)
53735424 .. 53742045
6622 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019575

Sequence Viewer

Length: 2313 bp
ATGGCTTCTCCTCCTTCAACTGTTAATCAGGGAGTTGTGCAAAAGGAAAACCGGATTGTATTGCCCAATTCTTTCCAGACTGTCATCAATGGGGGCTTCGTGCATGGGACCTTCAGCTTTAAAGGTGATCGTACCAGCCTGTCCCTCCCTCGTGCACCTTTTGGAGTGAAAGATGCTACCAAAGTGCGCAAACCAGTGGTTATTAGAAAAGAAAAGAGCATCGTTTCGGAGAAGGAGAGAAGTTCACAACCTTTGGTTCCGATTGATACAAATATTGAGCCTGAGGAGGTTGCAGAGCTTATACCATCTGAGGGTATGAGTACAATGCAGACAGGGGACTCTGTAGGTGTGAATACACATCAAATACTAGATGCGACTGTTTGTGTCAACCAGGAGACAATTAATGTGACTGATATACACAGGGAAGTTGAGAAACAGGTTGATGACACTGCTGATGTGGATGGCGTGCCTGTGGCACCTGTTCTGGCAGCTAATACGGTGCCAGAATTTGCAGCTAATATAGTGCAGCAAGCTAAAGAAGGCCGGATTGTGGCTACTCGAGTTGATAAAAGGGGTAGGCCTTCTTCTCCTACTGTATCTCCTAAGAAGTTCAAACGGGGAAGTACTGAGCTGTTTCCTAGCCCCATGGATGAGGATATCCCTATCAAAAAGCTAAAGATGCCAATTAAGTGGCAGTCATTCGTTCCATTTCCATCAGCAGTACTTGAGCTCGGATTCCGTTACGGACAAGTCTCTGCCATCGCTTTCTCTTCTGCATGCGCCTCTAACCTCTTCTCCTTCTTTTTCTTCTTCACCATCACATCACAGAGGTCCTCTGCATTTTCACCTCAGGTTCTAGAAAGTTCTTCCCAAGAACTGCAACAACCCGATTCAGATCACTTTTCCTCTGATTATGATGGAAGCTTTCTGGATGACTTTTCAGATTTGGAAAGCTCTGGCGATGAGGAAATCGTGAGTCCTACATCCGAGGAGGAAAAGCTACTCTTGTCCTTGATCAAAATGGGGTACACAGAAGAGGAGGCTTCCATAGCTATAGAAAGATGTGGTCTTAGCTCTTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCATTTGGCAAAGGCAGAAGATCCCCTTCTTCCTCTAGAAAAGAAGCCTGGACTAAAACATTTGCACACTGATTATCCTAAGAAGAAAAGGAAACTTTTGGTTGATGAAGTGTCGAAAAGGAAAAGGCAAAATAGGCTCATAGGCGAGGATAATGAGACAGTTCGTCTCCCAAATCCAATGATTGGGTTTGGTATTCCAACTGGACCATGCCAATCTTACAGAAATCTTCCAAAGGCAGCCATAGGACCTCCCTATTTTTATTATGAGAATGTAGCCCTCACTCCTAAGGGGGTTTGGGACACTATATCAAGATTCCTATATGATGTTCCACCTGAATTTGTTGACTCTAAGTACTTCTGTGCTGCTGCTAGAAAAAGAGGTTATGTTCACAATCTTCCAATCGAGAATAGATTCCCTCTCGTTCCACTTCCCCCACAAACCATACATGAGGCATTGCCCATGTCAAGAAAGTGGTGGCCTTCTTGGGACACACGGACAAAGCTAAATTGCTTACAAACTTGCCATTCAAGTGCAAAATTAACAGAGAGGATCATGAAGGCTCTTGAGGGCTATGATGACATTCCACCAGAGAGAGTCCAGCGGGGTGTAATGCATGAATGCCGTAAGTGGAATCTAGTGTGGGTTGGAAAAAATAGGGTCGCCCCACTTGAGCCTGATGAATTTGAAAAGCTTTTGGGGTTTCCAAAGGACCACACCCGGGGAATAAGCAGGACAGATAGATATAAATCACTCGGGAATGCATTTCAGATTGACACCGTCGCATACCACTTCTCTGTTTTGCGAGACATGTTTCCCAATGGCATCAATGTCCTCTCCCTTTTCTCTGGGATAGGTGGGGCGGAAATAGCCCTTCATCGGCTTGGTATCCGTATGAAGAATGTTGTGGCTCTAGAGATTTCAGAAGTTAACAGAAACGTTCTGAGGTGTTGGTGGGAGCAAACAAACCAGAGGGGGAATTTGATTCACCTTCCTGATGTGCAAGAGCTTAATGCTGACCGCTTGGAACAGTTTATGAGTTCATTTGGTGGCTTTGATCTAGTAGTTGGTGGCAGTCCCTGCAACAATCTTACAGGTAGCAATCGATATCATAGAGATGGACTCGAGGGTAAAGAATCTTCCCTATTCTATCATTTCTTTCGTATACTGGACTTGGTTAAGTCTATTATGGCAGGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

771

Amino Acids

86.25

Weight (kDa)

6.42

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 647 - 761 4.1e-08 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 188
AccB1I GGYRCC 2 cut(s) 475, 499
AccB7I CCANNNNNTGG 1 cut(s) 1295
AccI GTMKAC 1 cut(s) 2275
AciI CCGC 3 cut(s) 1714, 1973, 2131
AclI AACGTT 1 cut(s) 2049
AclWI GGATC 2 cut(s) 1127, 1670
AcsI RAATTY 4 cut(s) 506, 1448, 1793, 2089
AcuI CTGAAG 1 cut(s) 97
AfaI GTAC 6 cut(s) 133, 322, 625, 723, 1028, 1466
AfiI CCNNNNNNNGG 5 cut(s) 311, 550, 1295, 1831, 1989
AflIII ACRYGT 1 cut(s) 1920
AgsI TTSAA 4 cut(s) 18, 613, 1641, 1799
AhdI GACNNNNNGTC 1 cut(s) 1275
AjnI CCWGG 2 cut(s) 390, 1159
Alw21I GWGCWC 2 cut(s) 157, 732
Alw26I GTCTC 5 cut(s) 389, 757, 1262, 1283, 1911
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 2 cut(s) 1127, 1670
AlwNI CAGNNNCTG 1 cut(s) 2190
Ama87I CYCGRG 4 cut(s) 558, 1830, 1865, 2234
AoxI GGCC 3 cut(s) 541, 578, 1589
ApaLI GTGCAC 1 cut(s) 153
ApeKI GCWGC 7 cut(s) 488, 512, 526, 1109, 1349, 1475, 1478
ApoI RAATTY 4 cut(s) 506, 1448, 1793, 2089
AseI ATTAAT 1 cut(s) 402
Asp700I GAANNNNTTC 1 cut(s) 1523
AspLEI GCGC 2 cut(s) 189, 782
AspS9I GGNCC 5 cut(s) 108, 831, 1316, 1358, 1822
AsuC2I CCSGG 2 cut(s) 1831, 1832
AsuHPI GGTGA 4 cut(s) 137, 805, 837, 2090
AvaI CYCGRG 4 cut(s) 558, 1830, 1865, 2234
AvaII GGWCC 5 cut(s) 108, 831, 1316, 1358, 1822
AxyI CCTNAGG 3 cut(s) 282, 849, 1398
BaeGI GKGCMC 1 cut(s) 157
BanI GGYRCC 2 cut(s) 475, 499
BanII GRGCYC 1 cut(s) 732
BarI GAAGNNNNNNTAC 4 cut(s) 568, 600, 2233, 2265
BauI CACGAG 1 cut(s) 150
Bbv12I GWGCWC 2 cut(s) 157, 732
BbvI GCAGC 7 cut(s) 500, 524, 538, 1096, 1361, 1462, 1465
BccI CCATC 7 cut(s) 313, 455, 721, 767, 824, 911, 2222
BceAI ACGGC 1 cut(s) 1719
BciT130I CCWGG 2 cut(s) 392, 1161
BciVI GTATCC 1 cut(s) 2009
BclI TGATCA 1 cut(s) 1014
BcnI CCSGG 2 cut(s) 1831, 1832
BcoDI GTCTC 5 cut(s) 389, 757, 1262, 1283, 1911
BfaI CTAG 8 cut(s) 368, 639, 857, 1148, 1482, 1748, 2024, 2171
BfmI CTRYAG 2 cut(s) 342, 1053
BfuI GTATCC 1 cut(s) 2009
BisI GCNGC 7 cut(s) 489, 513, 527, 1110, 1350, 1476, 1479
BlsI GCNGC 7 cut(s) 490, 514, 528, 1111, 1351, 1477, 1480
BmcAI AGTACT 3 cut(s) 625, 723, 1466
Bme1390I CCNGG 4 cut(s) 392, 1161, 1831, 1832
Bme18I GGWCC 5 cut(s) 108, 831, 1316, 1358, 1822
BmeRI GACNNNNNGTC 1 cut(s) 1275
BmeT110I CYCGRG 4 cut(s) 558, 1830, 1865, 2234
BmgT120I GGNCC 5 cut(s) 108, 831, 1316, 1358, 1822
BmiI GGNNCC 4 cut(s) 109, 258, 477, 501
BmrFI CCNGG 4 cut(s) 392, 1161, 1831, 1832
BmsI GCATC 5 cut(s) 163, 228, 361, 669, 1944
BplI GAGNNNNNCTC 2 cut(s) 2217, 2249
BpuEI CTTGAG 3 cut(s) 746, 1697, 1802
BpuMI CCSGG 2 cut(s) 1831, 1832
Bsa29I ATCGAT 1 cut(s) 2215
BsaBI GATNNNNATC 2 cut(s) 894, 1858
BsaJI CCNNGG 4 cut(s) 645, 987, 1830, 1831
BsaWI WCCGGW 1 cut(s) 51
BsaXI ACNNNNNCTCC 4 cut(s) 583, 613, 779, 809
Bsc4I CCNNNNNNNGG 5 cut(s) 311, 550, 1295, 1831, 1989
Bse1I ACTGG 3 cut(s) 194, 1318, 2283
Bse21I CCTNAGG 3 cut(s) 282, 849, 1398
Bse3DI GCAATG 1 cut(s) 1565
Bse8I GATNNNNATC 2 cut(s) 894, 1858
BseBI CCWGG 2 cut(s) 392, 1161
BseCI ATCGAT 1 cut(s) 2215
BseDI CCNNGG 4 cut(s) 645, 987, 1830, 1831
BseGI GGATG 4 cut(s) 466, 655, 937, 983
BseJI GATNNNNATC 2 cut(s) 894, 1858
BseLI CCNNNNNNNGG 5 cut(s) 311, 550, 1295, 1831, 1989
BseMI GCAATG 1 cut(s) 1565
BseMII CTCAG 5 cut(s) 273, 300, 618, 863, 2045
BseNI ACTGG 3 cut(s) 194, 1318, 2283
BseRI GAGGAG 3 cut(s) 299, 1004, 1052
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 7 cut(s) 500, 524, 538, 1096, 1361, 1462, 1465
BsgI GTGCAG 1 cut(s) 545
BshFI GGCC 3 cut(s) 543, 580, 1591
BshNI GGYRCC 2 cut(s) 475, 499
BshVI ATCGAT 1 cut(s) 2215
BsiHKAI GWGCWC 2 cut(s) 157, 732
BsiHKCI CYCGRG 4 cut(s) 558, 1830, 1865, 2234
BsiSI CCGG 3 cut(s) 52, 544, 1831
BslFI GGGAC 6 cut(s) 121, 127, 350, 1424, 1613, 2172
BslI CCNNNNNNNGG 5 cut(s) 311, 550, 1295, 1831, 1989
BsmAI GTCTC 5 cut(s) 389, 757, 1262, 1283, 1911
BsmBI CGTCTC 1 cut(s) 1283
BsmFI GGGAC 6 cut(s) 121, 127, 350, 1424, 1613, 2172
BsmI GAATGC 2 cut(s) 1736, 1876
BsnI GGCC 3 cut(s) 543, 580, 1591
BsoBI CYCGRG 4 cut(s) 558, 1830, 1865, 2234
Bsp1286I GDGCHC 2 cut(s) 157, 732
Bsp143I GATC 6 cut(s) 127, 895, 1014, 1132, 1662, 2167
Bsp19I CCATGG 1 cut(s) 645
BspACI CCGC 3 cut(s) 1714, 1973, 2131
BspANI GGCC 3 cut(s) 543, 580, 1591
BspCNI CTCAG 5 cut(s) 274, 301, 619, 862, 2046
BspDI ATCGAT 1 cut(s) 2215
BspHI TCATGA 1 cut(s) 1665
BspLI GGNNCC 4 cut(s) 109, 258, 477, 501
BspPI GGATC 2 cut(s) 1127, 1670
BspQI GCTCTTC 1 cut(s) 1081
BspT107I GGYRCC 2 cut(s) 475, 499
BsrDI GCAATG 1 cut(s) 1565
BsrI ACTGG 3 cut(s) 194, 1318, 2283
BssECI CCNNGG 4 cut(s) 645, 987, 1830, 1831
BssMI GATC 6 cut(s) 127, 895, 1014, 1132, 1662, 2167
BssNAI GTATAC 1 cut(s) 2276
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 645
Bst1107I GTATAC 1 cut(s) 2276
Bst2BI CACGAG 1 cut(s) 150
Bst2UI CCWGG 2 cut(s) 392, 1161
Bst4CI ACNGT 8 cut(s) 22, 82, 379, 499, 595, 1273, 1891, 2142
Bst6I CTCTTC 4 cut(s) 775, 797, 1029, 1081
BstAPI GCANNNNNTGC 1 cut(s) 2190
BstC8I GCNNGC 3 cut(s) 467, 531, 778
BstDSI CCRYGG 1 cut(s) 645
BstF5I GGATG 4 cut(s) 466, 655, 937, 983
BstHHI GCGC 2 cut(s) 189, 782
BstKTI GATC 6 cut(s) 130, 898, 1017, 1135, 1665, 2170
BstMAI GTCTC 5 cut(s) 389, 757, 1262, 1283, 1911
BstMBI GATC 6 cut(s) 127, 895, 1014, 1132, 1662, 2167
BstMWI GCNNNNNNNGC 6 cut(s) 679, 1049, 1118, 1246, 1979, 2190
BstNI CCWGG 2 cut(s) 392, 1161
BstNSI RCATGY 2 cut(s) 780, 1924
BstSCI CCNGG 4 cut(s) 390, 1159, 1829, 1830
BstSFI CTRYAG 2 cut(s) 342, 1053
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 7 cut(s) 500, 524, 538, 1096, 1361, 1462, 1465
BstX2I RGATCY 1 cut(s) 1132
BstXI CCANNNNNNTGG 1 cut(s) 690
BstYI RGATCY 1 cut(s) 1132
BstZ17I GTATAC 1 cut(s) 2276
Bsu15I ATCGAT 1 cut(s) 2215
Bsu36I CCTNAGG 3 cut(s) 282, 849, 1398
BsuI GTATCC 1 cut(s) 2009
BsuRI GGCC 3 cut(s) 543, 580, 1591
BsuTUI ATCGAT 1 cut(s) 2215
BtgI CCRYGG 1 cut(s) 645
BtgZI GCGATG 2 cut(s) 745, 975
BtsCI GGATG 4 cut(s) 466, 655, 937, 983
BtsI GCAGTG 1 cut(s) 447
BtsIMutI CAGTG 3 cut(s) 201, 447, 1179
Cac8I GCNNGC 3 cut(s) 467, 531, 778
CaiI CAGNNNCTG 1 cut(s) 2190
CciI TCATGA 1 cut(s) 1665
CfoI GCGC 2 cut(s) 189, 782
Cfr13I GGNCC 5 cut(s) 108, 831, 1316, 1358, 1822
Cfr9I CCCGGG 1 cut(s) 1830
ClaI ATCGAT 1 cut(s) 2215
Csp6I GTAC 6 cut(s) 132, 321, 624, 722, 1027, 1465
CviAII CATG 9 cut(s) 104, 646, 777, 1320, 1559, 1573, 1666, 1727, 1921
CviQI GTAC 6 cut(s) 132, 321, 624, 722, 1027, 1465
DpnI GATC 6 cut(s) 129, 897, 1016, 1134, 1664, 2169
DpnII GATC 6 cut(s) 127, 895, 1014, 1132, 1662, 2167
DraI TTTAAA 1 cut(s) 121
DriI GACNNNNNGTC 1 cut(s) 1275
Eam1104I CTCTTC 4 cut(s) 775, 797, 1029, 1081
Eam1105I GACNNNNNGTC 1 cut(s) 1275
EarI CTCTTC 4 cut(s) 775, 797, 1029, 1081
EciI GGCGGA 1 cut(s) 1988
Ecl136II GAGCTC 1 cut(s) 730
Eco130I CCWWGG 1 cut(s) 645
Eco147I AGGCCT 1 cut(s) 580
Eco24I GRGCYC 1 cut(s) 732
Eco32I GATATC 2 cut(s) 658, 2219
Eco47I GGWCC 5 cut(s) 108, 831, 1316, 1358, 1822
Eco53kI GAGCTC 1 cut(s) 730
Eco57I CTGAAG 1 cut(s) 97
Eco81I CCTNAGG 3 cut(s) 282, 849, 1398
Eco88I CYCGRG 4 cut(s) 558, 1830, 1865, 2234
EcoICRI GAGCTC 1 cut(s) 730
EcoO109I RGGNCCY 3 cut(s) 108, 831, 1358
EcoRII CCWGG 2 cut(s) 390, 1159
EcoRV GATATC 2 cut(s) 658, 2219
EcoT14I CCWWGG 1 cut(s) 645
EcoT22I ATGCAT 2 cut(s) 1728, 1876
EcoT38I GRGCYC 1 cut(s) 732
ErhI CCWWGG 1 cut(s) 645
Esp3I CGTCTC 1 cut(s) 1283
FaeI CATG 9 cut(s) 107, 649, 780, 1323, 1562, 1576, 1669, 1730, 1924
FalI AAGNNNNNCTT 4 cut(s) 989, 1021, 1122, 1154
FaqI GGGAC 6 cut(s) 121, 127, 350, 1424, 1613, 2172
FatI CATG 9 cut(s) 103, 645, 776, 1319, 1558, 1572, 1665, 1726, 1920
FauI CCCGC 1 cut(s) 1707
FbaI TGATCA 1 cut(s) 1014
FblI GTMKAC 1 cut(s) 2275
Fnu4HI GCNGC 7 cut(s) 489, 513, 527, 1110, 1350, 1476, 1479
FokI GGATG 4 cut(s) 473, 662, 944, 970
FriOI GRGCYC 1 cut(s) 732
Fsp4HI GCNGC 7 cut(s) 489, 513, 527, 1110, 1350, 1476, 1479
FspBI CTAG 8 cut(s) 368, 639, 857, 1148, 1482, 1748, 2024, 2171
FspI TGCGCA 1 cut(s) 188
GlaI GCGC 2 cut(s) 188, 781
GluI GCNGC 7 cut(s) 489, 513, 527, 1110, 1350, 1476, 1479
HaeIII GGCC 3 cut(s) 543, 580, 1591
HapII CCGG 3 cut(s) 52, 544, 1831
HhaI GCGC 2 cut(s) 189, 782
Hin1II CATG 9 cut(s) 107, 649, 780, 1323, 1562, 1576, 1669, 1730, 1924
Hin6I GCGC 2 cut(s) 187, 780
HinP1I GCGC 2 cut(s) 187, 780
HincII GTYRAC 3 cut(s) 388, 1456, 2041
HindII GTYRAC 3 cut(s) 388, 1456, 2041
HindIII AAGCTT 2 cut(s) 922, 1802
HpaI GTTAAC 1 cut(s) 2041
HpaII CCGG 3 cut(s) 52, 544, 1831
HphI GGTGA 4 cut(s) 137, 805, 837, 2090
Hpy166II GTNNAC 8 cut(s) 155, 245, 388, 1029, 1456, 1501, 2041, 2276
Hpy8I GTNNAC 8 cut(s) 155, 245, 388, 1029, 1456, 1501, 2041, 2276
Hpy99I CGWCG 1 cut(s) 1895
HpyCH4III ACNGT 8 cut(s) 22, 82, 379, 499, 595, 1273, 1891, 2142
HpyCH4IV ACGT 1 cut(s) 2049
HpyF10VI GCNNNNNNNGC 6 cut(s) 679, 1049, 1118, 1246, 1979, 2190
HpySE526I ACGT 1 cut(s) 2049
Hsp92II CATG 9 cut(s) 107, 649, 780, 1323, 1562, 1576, 1669, 1730, 1924
HspAI GCGC 2 cut(s) 187, 780
Ksp22I TGATCA 1 cut(s) 1014
KspAI GTTAAC 1 cut(s) 2041
Kzo9I GATC 6 cut(s) 127, 895, 1014, 1132, 1662, 2167
LguI GCTCTTC 1 cut(s) 1081
LmnI GCTCC 1 cut(s) 2068
Lsp1109I GCAGC 7 cut(s) 500, 524, 538, 1096, 1361, 1462, 1465
LweI GCATC 5 cut(s) 163, 228, 361, 669, 1944
MaeI CTAG 8 cut(s) 368, 639, 857, 1148, 1482, 1748, 2024, 2171
MaeII ACGT 1 cut(s) 2049
MaeIII GTNAC 2 cut(s) 406, 740
MalI GATC 6 cut(s) 129, 897, 1016, 1134, 1664, 2169
MboI GATC 6 cut(s) 127, 895, 1014, 1132, 1662, 2167
MflI RGATCY 1 cut(s) 1132
MhlI GDGCHC 2 cut(s) 157, 732
MlyI GAGTC 5 cut(s) 332, 985, 1451, 1716, 2226
MmeI TCCRAC 2 cut(s) 1334, 1738
Mph1103I ATGCAT 2 cut(s) 1728, 1876
MroXI GAANNNNTTC 1 cut(s) 1523
MseI TTAA 8 cut(s) 24, 120, 402, 687, 1652, 2040, 2121, 2289
MslI CAYNNNNRTG 2 cut(s) 1641, 2226
MspA1I CMGCKG 1 cut(s) 1714
MspI CCGG 3 cut(s) 52, 544, 1831
MspR9I CCNGG 4 cut(s) 392, 1161, 1831, 1832
Mva1269I GAATGC 2 cut(s) 1736, 1876
MvaI CCWGG 2 cut(s) 392, 1161
MwoI GCNNNNNNNGC 6 cut(s) 679, 1049, 1118, 1246, 1979, 2190
NciI CCSGG 2 cut(s) 1831, 1832
NcoI CCATGG 1 cut(s) 645
NdeII GATC 6 cut(s) 127, 895, 1014, 1132, 1662, 2167
NlaIII CATG 9 cut(s) 107, 649, 780, 1323, 1562, 1576, 1669, 1730, 1924
NlaIV GGNNCC 4 cut(s) 109, 258, 477, 501
NmuCI GTSAC 1 cut(s) 406
NsbI TGCGCA 1 cut(s) 188
NsiI ATGCAT 2 cut(s) 1728, 1876
NspI RCATGY 2 cut(s) 780, 1924
PaeI GCATGC 1 cut(s) 780
PaeR7I CTCGAG 2 cut(s) 558, 2234
PagI TCATGA 1 cut(s) 1665
PceI AGGCCT 1 cut(s) 580
PciI ACATGT 1 cut(s) 1920
PciSI GCTCTTC 1 cut(s) 1081
PctI GAATGC 2 cut(s) 1736, 1876
PdmI GAANNNNTTC 1 cut(s) 1523
PfeI GAWTC 7 cut(s) 735, 890, 1425, 1524, 1744, 2095, 2246
PflFI GACNNNGTC 1 cut(s) 1889
PflMI CCANNNNNTGG 1 cut(s) 1295
PkrI GCNGC 7 cut(s) 490, 514, 528, 1111, 1351, 1477, 1480
PleI GAGTC 5 cut(s) 332, 984, 1451, 1715, 2226
PpsI GAGTC 5 cut(s) 332, 984, 1451, 1715, 2226
PpuMI RGGWCCY 3 cut(s) 108, 831, 1358
PscI ACATGT 1 cut(s) 1920
PshBI ATTAAT 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 732
Psp1406I AACGTT 1 cut(s) 2049
Psp5II RGGWCCY 3 cut(s) 108, 831, 1358
Psp6I CCWGG 2 cut(s) 390, 1159
PspGI CCWGG 2 cut(s) 390, 1159
PspN4I GGNNCC 4 cut(s) 109, 258, 477, 501
PspPI GGNCC 5 cut(s) 108, 831, 1316, 1358, 1822
PspPPI RGGWCCY 3 cut(s) 108, 831, 1358
PspXI VCTCGAGB 2 cut(s) 558, 2234
PstNI CAGNNNCTG 1 cut(s) 2190
PsuI RGATCY 1 cut(s) 1132
PsyI GACNNNGTC 1 cut(s) 1889
RsaI GTAC 6 cut(s) 133, 322, 625, 723, 1028, 1466
RsaNI GTAC 6 cut(s) 132, 321, 624, 722, 1027, 1465
RseI CAYNNNNRTG 2 cut(s) 1641, 2226
SacI GAGCTC 1 cut(s) 732
SapI GCTCTTC 1 cut(s) 1081
SaqAI TTAA 8 cut(s) 24, 120, 402, 687, 1652, 2040, 2121, 2289
SatI GCNGC 7 cut(s) 489, 513, 527, 1110, 1350, 1476, 1479
Sau3AI GATC 6 cut(s) 127, 895, 1014, 1132, 1662, 2167
Sau96I GGNCC 5 cut(s) 108, 831, 1316, 1358, 1822
ScaI AGTACT 3 cut(s) 625, 723, 1466
SchI GAGTC 5 cut(s) 332, 985, 1451, 1716, 2226
ScrFI CCNGG 4 cut(s) 392, 1161, 1831, 1832
SduI GDGCHC 2 cut(s) 157, 732
SfaNI GCATC 5 cut(s) 163, 228, 361, 669, 1944
SfcI CTRYAG 2 cut(s) 342, 1053
Sfr274I CTCGAG 2 cut(s) 558, 2234
SinI GGWCC 5 cut(s) 108, 831, 1316, 1358, 1822
SlaI CTCGAG 2 cut(s) 558, 2234
SmaI CCCGGG 1 cut(s) 1832
SmiMI CAYNNNNRTG 2 cut(s) 1641, 2226
SmlI CTYRAG 5 cut(s) 558, 725, 1676, 1781, 2234
SmoI CTYRAG 5 cut(s) 558, 725, 1676, 1781, 2234
SphI GCATGC 1 cut(s) 780
SseBI AGGCCT 1 cut(s) 580
SsiI CCGC 3 cut(s) 1714, 1973, 2131
SspI AATATT 1 cut(s) 274
SspMI CTAG 8 cut(s) 368, 639, 857, 1148, 1482, 1748, 2024, 2171
SstI GAGCTC 1 cut(s) 732
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 4 cut(s) 390, 1159, 1829, 1830
StyI CCWWGG 1 cut(s) 645
TaaI ACNGT 8 cut(s) 22, 82, 379, 499, 595, 1273, 1891, 2142
TaiI ACGT 1 cut(s) 2052
TaqI TCGA 5 cut(s) 559, 1226, 1515, 2215, 2235
TatI WGTACW 4 cut(s) 320, 623, 721, 1464
TfiI GAWTC 7 cut(s) 735, 890, 1425, 1524, 1744, 2095, 2246
Tru1I TTAA 8 cut(s) 24, 120, 402, 687, 1652, 2040, 2121, 2289
Tru9I TTAA 8 cut(s) 24, 120, 402, 687, 1652, 2040, 2121, 2289
TscAI CASTG 3 cut(s) 201, 454, 1186
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 7 cut(s) 488, 512, 526, 1109, 1349, 1475, 1478
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 7 cut(s) 1233, 1682, 1743, 1806, 1976, 2021, 2142
TspGWI ACGGA 4 cut(s) 728, 759, 1621, 1991
TspMI CCCGGG 1 cut(s) 1830
TspRI CASTG 3 cut(s) 201, 454, 1186
Tth111I GACNNNGTC 1 cut(s) 1889
Van91I CCANNNNNTGG 1 cut(s) 1295
VneI GTGCAC 1 cut(s) 153
VpaK11BI GGWCC 5 cut(s) 108, 831, 1316, 1358, 1822
VspI ATTAAT 1 cut(s) 402
XapI RAATTY 4 cut(s) 506, 1448, 1793, 2089
XbaI TCTAGA 3 cut(s) 856, 1147, 2023
XceI RCATGY 2 cut(s) 780, 1924
XhoI CTCGAG 2 cut(s) 558, 2234
XmaI CCCGGG 1 cut(s) 1830
XmiI GTMKAC 1 cut(s) 2275
XmnI GAANNNNTTC 1 cut(s) 1523
XspI CTAG 8 cut(s) 368, 639, 857, 1148, 1482, 1748, 2024, 2171
ZrmI AGTACT 3 cut(s) 625, 723, 1466
Zsp2I ATGCAT 2 cut(s) 1728, 1876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.