Rw1G004090

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000338
Physical Location & Seq
Forward (+)
7678498 .. 7684807
6310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004090.1

Sequence Viewer

Length: 1578 bp
ATGGTTGGAGTGTGGCATGCTGTTGCTGCATGGACATGGGATGCTCAGGATGAAACATGTGGACCTTCCCAAACCAAGGTTTCTGATCATTTTGTCAGCATGGGATTTTCTCATAATATGGTTGCCAAAGCCATCCAAGAACATGGACTTGAGGATGCAGATTCAATTCTTGAAATCCTCCTCTTATATAACGCTCTTGAAAGTAGTCCTCCGGAGCAACATTCTCTTCCAGAGCTGCATTCTCCTCCAGAACAGTGCTGTCCTCAAGAACAGCAACTAGTTGACTCGGATCTCTTTTATTCAGATTATGATGGGGGCTTTCTAGACAGTCTTTCATACCTCTATAGCTCTGATGATGAGGTAACCATCGATTGTACATCTGAGGCAGAGACAAAACTTATGTCGTCGGTTAAAATGGGAAAAATGGCAGGTATTGACTCGTCACTTGTTGATTTGACTGATTTCATATCAGCTGCTCAAATTTCAAAGGCAGAAGATGGCCATCTTCCCCTTGAAGAGATGCGTACACATCTGCGGAGTGAGTATCCTGAGAAGAATAAATGGAAACTTTGTGAATCTTCATTGCTGAAAAGGAAAAGGATTACGGGACATGGAAACCAGATCATAGGGGAAGACGCTGGTGCAATTCATCTCCCAAATCCAATGAATGGGTTTGGGACTCCAGCTGAACTCTGTCAAATCCACAGAAGTCTTCCAAAGGCGGCCACAGGACCTCCTTATTTTTGTGCTGCAAGAAAAAGGGGTTATGTTCACAATTTTCCAATCAAAAACAGATTTCCTCTCGTTTCCCTTCCACCACAAACTATATTTGATGCGTTTACCATGACAAGGAGGTGGCTAAATTGCTTACAGGCTTGCATTGCTAGTGCAACAATTACTGAGAGGATCATGAAGGCTCTCAAAGACTATGACGAAGATGAAATGCCACCTGAAAGTGTCCGTGGCTATGTTCTTTATGAATGCCGCAAGTGGAATCTTGTTTGGGTAGGAAAGAAAAAGATGGCCCCACTTGAGCCTGATCAAGTGGAAATGCTTTTGGGGTTCCCAAGGGGACCTGACAAGGATTTTCACTTTTATTGCATACTCTTTGCTATTACATTTTACTTACGGGCTCCACTTCTTCAGATTGATACACTGGCTTATCACCTGTCAGTTTTGAAAGATCGATTTCCCAATGGCATCAATGTCTCTCTCTTCTCTGGGATTGGCGGTGCAGAGATAGCTCTTTACCGGCTTGGTATCCCGATGAAGAATTTTGTGTCAGTGGAGATTTCAGAGGTGGGCAGAAATGTTGTGAGGTGTTGGTGGGAGCAGACAATCGAGAGAGGGAACTTGTATCACCTTGCTGATGTGCAAGAGTTGAATGCTGACTGCTTAGAGCATTATATCAGTTCCTTTGGTGGGTTTGATCTAGTGATTTGTGGCGGTCCATGTAACAATCTTGCGGGTAGCAACAGGCACCATCGGGATGGGCTTGAGGGTAAAGAATCTTCTCTTTTTTATGACCCTTTTTGTATATTGGATTTGGTCAAGGGTATTATGGCAAGATACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

525

Amino Acids

59.04

Weight (kDa)

5.31

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 419
AccB1I GGYRCC 1 cut(s) 1479
AccB7I CCANNNNNTGG 1 cut(s) 668
AccIII TCCGGA 1 cut(s) 211
AciI CCGC 6 cut(s) 535, 722, 985, 1230, 1446, 1466
AclWI GGATC 2 cut(s) 297, 914
AcoI YGGCCR 2 cut(s) 499, 723
AcsI RAATTY 2 cut(s) 480, 1273
AcuI CTGAAG 1 cut(s) 1127
AfaI GTAC 2 cut(s) 376, 526
AfiI CCNNNNNNNGG 4 cut(s) 76, 668, 849, 1422
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 7 cut(s) 165, 173, 200, 486, 515, 1180, 1384
AhlI ACTAGT 1 cut(s) 277
AluBI AGCT 5 cut(s) 235, 348, 473, 686, 1244
AluI AGCT 5 cut(s) 235, 348, 473, 686, 1244
Alw26I GTCTC 2 cut(s) 383, 1213
AlwI GGATC 2 cut(s) 297, 914
Aor13HI TCCGGA 1 cut(s) 211
AoxI GGCC 3 cut(s) 499, 723, 1023
ApeKI GCWGC 4 cut(s) 26, 235, 473, 749
ApoI RAATTY 2 cut(s) 480, 1273
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
AspS9I GGNCC 5 cut(s) 62, 731, 1024, 1073, 1448
AsuHPI GGTGA 2 cut(s) 1157, 1352
AvaII GGWCC 4 cut(s) 62, 731, 1073, 1448
BalI TGGCCA 1 cut(s) 501
BanI GGYRCC 1 cut(s) 1479
BanII GRGCYC 1 cut(s) 1135
BarI GAAGNNNNNNTAC 2 cut(s) 1495, 1527
BbsI GAAGAC 2 cut(s) 639, 704
BbvI GCAGC 4 cut(s) 13, 222, 460, 736
BccI CCATC 8 cut(s) 140, 305, 374, 491, 510, 1015, 1484, 1491
BciVI GTATCC 2 cut(s) 555, 1271
BclI TGATCA 2 cut(s) 85, 1039
BcoDI GTCTC 2 cut(s) 383, 1213
BcuI ACTAGT 1 cut(s) 277
BfaI CTAG 4 cut(s) 278, 323, 885, 1433
BfmI CTRYAG 1 cut(s) 343
BfuAI ACCTGC 1 cut(s) 419
BfuI GTATCC 2 cut(s) 555, 1271
BisI GCNGC 6 cut(s) 27, 236, 474, 723, 750, 985
BlsI GCNGC 6 cut(s) 28, 237, 475, 724, 751, 986
Bme18I GGWCC 4 cut(s) 62, 731, 1073, 1448
BmgT120I GGNCC 5 cut(s) 62, 731, 1024, 1073, 1448
BmiI GGNNCC 5 cut(s) 1026, 1064, 1074, 1134, 1481
BmsI GCATC 5 cut(s) 31, 145, 510, 823, 1209
BpiI GAAGAC 2 cut(s) 639, 704
BpmI CTGGAG 2 cut(s) 231, 666
Bpu10I CCTNAGC 1 cut(s) 45
BpuEI CTTGAG 4 cut(s) 170, 249, 1052, 1517
Bsa29I ATCGAT 2 cut(s) 369, 1186
BsaBI GATNNNNATC 1 cut(s) 501
BsaJI CCNNGG 3 cut(s) 75, 961, 1067
BsaWI WCCGGW 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 718, 748
Bsc4I CCNNNNNNNGG 4 cut(s) 76, 668, 849, 1422
Bse118I RCCGGY 1 cut(s) 1251
Bse1I ACTGG 1 cut(s) 1161
Bse3DI GCAATG 2 cut(s) 581, 879
Bse8I GATNNNNATC 1 cut(s) 501
BseAI TCCGGA 1 cut(s) 211
BseCI ATCGAT 2 cut(s) 369, 1186
BseDI CCNNGG 3 cut(s) 75, 961, 1067
BseGI GGATG 5 cut(s) 46, 55, 132, 160, 1495
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 4 cut(s) 76, 668, 849, 1422
BseMI GCAATG 2 cut(s) 581, 879
BseMII CTCAG 4 cut(s) 59, 372, 540, 891
BseNI ACTGG 1 cut(s) 1161
BseRI GAGGAG 2 cut(s) 170, 234
BseXI GCAGC 4 cut(s) 13, 222, 460, 736
BsgI GTGCAG 1 cut(s) 1254
BshFI GGCC 3 cut(s) 501, 725, 1025
BshNI GGYRCC 1 cut(s) 1479
BshVI ATCGAT 2 cut(s) 369, 1186
BsiSI CCGG 2 cut(s) 212, 1252
BslFI GGGAC 3 cut(s) 621, 691, 1086
BslI CCNNNNNNNGG 4 cut(s) 76, 668, 849, 1422
BsmAI GTCTC 2 cut(s) 383, 1213
BsmFI GGGAC 3 cut(s) 621, 691, 1086
BsmI GAATGC 3 cut(s) 238, 986, 1390
BsnI GGCC 3 cut(s) 501, 725, 1025
Bsp1286I GDGCHC 1 cut(s) 1135
Bsp13I TCCGGA 1 cut(s) 211
Bsp1407I TGTACA 1 cut(s) 374
Bsp143I GATC 7 cut(s) 85, 289, 621, 906, 1039, 1183, 1429
BspACI CCGC 6 cut(s) 535, 722, 985, 1230, 1446, 1466
BspANI GGCC 3 cut(s) 501, 725, 1025
BspCNI CTCAG 4 cut(s) 58, 373, 541, 892
BspDI ATCGAT 2 cut(s) 369, 1186
BspEI TCCGGA 1 cut(s) 211
BspHI TCATGA 1 cut(s) 909
BspLI GGNNCC 5 cut(s) 1026, 1064, 1074, 1134, 1481
BspMI ACCTGC 1 cut(s) 419
BspPI GGATC 2 cut(s) 297, 914
BspT107I GGYRCC 1 cut(s) 1479
BsrDI GCAATG 2 cut(s) 581, 879
BsrFI RCCGGY 1 cut(s) 1251
BsrGI TGTACA 1 cut(s) 374
BsrI ACTGG 1 cut(s) 1161
BssAI RCCGGY 1 cut(s) 1251
BssECI CCNNGG 3 cut(s) 75, 961, 1067
BssMI GATC 7 cut(s) 85, 289, 621, 906, 1039, 1183, 1429
BssT1I CCWWGG 2 cut(s) 75, 1067
Bst4CI ACNGT 2 cut(s) 255, 329
Bst6I CTCTTC 3 cut(s) 231, 510, 1220
BstAUI TGTACA 1 cut(s) 374
BstC8I GCNNGC 2 cut(s) 18, 877
BstDEI CTNAG 5 cut(s) 45, 381, 549, 900, 1396
BstDSI CCRYGG 1 cut(s) 961
BstEII GGTNACC 1 cut(s) 361
BstF5I GGATG 5 cut(s) 46, 55, 132, 160, 1495
BstKTI GATC 7 cut(s) 88, 292, 624, 909, 1042, 1186, 1432
BstMAI GTCTC 2 cut(s) 383, 1213
BstMBI GATC 7 cut(s) 85, 289, 621, 906, 1039, 1183, 1429
BstMWI GCNNNNNNNGC 3 cut(s) 26, 881, 1241
BstNSI RCATGY 2 cut(s) 20, 60
BstPI GGTNACC 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 343
BstV1I GCAGC 4 cut(s) 13, 222, 460, 736
BstV2I GAAGAC 2 cut(s) 639, 704
BstX2I RGATCY 1 cut(s) 289
BstXI CCANNNNNNTGG 2 cut(s) 143, 1490
BstYI RGATCY 1 cut(s) 289
Bsu15I ATCGAT 2 cut(s) 369, 1186
BsuI GTATCC 2 cut(s) 555, 1271
BsuRI GGCC 3 cut(s) 501, 725, 1025
BsuTUI ATCGAT 2 cut(s) 369, 1186
BtgI CCRYGG 1 cut(s) 961
BtsCI GGATG 5 cut(s) 46, 55, 132, 160, 1495
BtsIMutI CAGTG 3 cut(s) 260, 1154, 1290
BveI ACCTGC 1 cut(s) 419
Cac8I GCNNGC 2 cut(s) 18, 877
CciI TCATGA 1 cut(s) 909
Cfr10I RCCGGY 1 cut(s) 1251
Cfr13I GGNCC 5 cut(s) 62, 731, 1024, 1073, 1448
ClaI ATCGAT 2 cut(s) 369, 1186
CseI GACGC 1 cut(s) 644
Csp6I GTAC 2 cut(s) 375, 525
CviQI GTAC 2 cut(s) 375, 525
DdeI CTNAG 5 cut(s) 45, 381, 549, 900, 1396
DpnI GATC 7 cut(s) 87, 291, 623, 908, 1041, 1185, 1431
DpnII GATC 7 cut(s) 85, 289, 621, 906, 1039, 1183, 1429
EaeI YGGCCR 2 cut(s) 499, 723
Eam1104I CTCTTC 3 cut(s) 231, 510, 1220
EarI CTCTTC 3 cut(s) 231, 510, 1220
Eco130I CCWWGG 2 cut(s) 75, 1067
Eco24I GRGCYC 1 cut(s) 1135
Eco47I GGWCC 4 cut(s) 62, 731, 1073, 1448
Eco57I CTGAAG 1 cut(s) 1127
Eco91I GGTNACC 1 cut(s) 361
EcoO109I RGGNCCY 2 cut(s) 731, 1073
EcoO65I GGTNACC 1 cut(s) 361
EcoT14I CCWWGG 2 cut(s) 75, 1067
EcoT38I GRGCYC 1 cut(s) 1135
ErhI CCWWGG 2 cut(s) 75, 1067
FaqI GGGAC 3 cut(s) 621, 691, 1086
FauI CCCGC 1 cut(s) 1459
FbaI TGATCA 2 cut(s) 85, 1039
Fnu4HI GCNGC 6 cut(s) 27, 236, 474, 723, 750, 985
FokI GGATG 5 cut(s) 53, 62, 119, 167, 1502
FriOI GRGCYC 1 cut(s) 1135
Fsp4HI GCNGC 6 cut(s) 27, 236, 474, 723, 750, 985
FspBI CTAG 4 cut(s) 278, 323, 885, 1433
GluI GCNGC 6 cut(s) 27, 236, 474, 723, 750, 985
GsuI CTGGAG 2 cut(s) 231, 666
HaeIII GGCC 3 cut(s) 501, 725, 1025
HapII CCGG 2 cut(s) 212, 1252
HgaI GACGC 1 cut(s) 644
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 7 cut(s) 161, 284, 437, 575, 679, 994, 1508
HpaII CCGG 2 cut(s) 212, 1252
HphI GGTGA 2 cut(s) 1157, 1352
Hpy166II GTNNAC 5 cut(s) 62, 283, 527, 772, 840
Hpy188I TCNGA 7 cut(s) 85, 289, 304, 352, 382, 1146, 1297
Hpy8I GTNNAC 5 cut(s) 62, 283, 527, 772, 840
Hpy99I CGWCG 1 cut(s) 409
HpyAV CCTTC 3 cut(s) 75, 821, 907
HpyCH4III ACNGT 2 cut(s) 255, 329
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 881, 1241
HpyF3I CTNAG 5 cut(s) 45, 381, 549, 900, 1396
Kpn2I TCCGGA 1 cut(s) 211
Ksp22I TGATCA 2 cut(s) 85, 1039
Kzo9I GATC 7 cut(s) 85, 289, 621, 906, 1039, 1183, 1429
LmnI GCTCC 3 cut(s) 214, 1138, 1330
Lsp1109I GCAGC 4 cut(s) 13, 222, 460, 736
LweI GCATC 5 cut(s) 31, 145, 510, 823, 1209
MaeI CTAG 4 cut(s) 278, 323, 885, 1433
MaeIII GTNAC 3 cut(s) 361, 441, 1454
MalI GATC 7 cut(s) 87, 291, 623, 908, 1041, 1185, 1431
MboI GATC 7 cut(s) 85, 289, 621, 906, 1039, 1183, 1429
MfeI CAATTG 1 cut(s) 1573
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 1 cut(s) 1135
MlsI TGGCCA 1 cut(s) 501
MluCI AATT 8 cut(s) 165, 480, 645, 775, 862, 894, 1273, 1573
MluNI TGGCCA 1 cut(s) 501
MlyI GAGTC 3 cut(s) 278, 431, 673
Mox20I TGGCCA 1 cut(s) 501
MroI TCCGGA 1 cut(s) 211
MscI TGGCCA 1 cut(s) 501
MseI TTAA 1 cut(s) 411
MslI CAYNNNNRTG 2 cut(s) 34, 1488
Msp20I TGGCCA 1 cut(s) 501
MspA1I CMGCKG 2 cut(s) 473, 686
MspI CCGG 2 cut(s) 212, 1252
MunI CAATTG 1 cut(s) 1573
Mva1269I GAATGC 3 cut(s) 238, 986, 1390
MwoI GCNNNNNNNGC 3 cut(s) 26, 881, 1241
NdeII GATC 7 cut(s) 85, 289, 621, 906, 1039, 1183, 1429
NlaIV GGNNCC 5 cut(s) 1026, 1064, 1074, 1134, 1481
NmuCI GTSAC 1 cut(s) 441
NspI RCATGY 2 cut(s) 20, 60
PaeI GCATGC 1 cut(s) 20
PagI TCATGA 1 cut(s) 909
PciI ACATGT 1 cut(s) 56
PctI GAATGC 3 cut(s) 238, 986, 1390
PfeI GAWTC 4 cut(s) 161, 575, 994, 1508
PflMI CCANNNNNTGG 1 cut(s) 668
PkrI GCNGC 6 cut(s) 28, 237, 475, 724, 751, 986
PleI GAGTC 3 cut(s) 278, 431, 673
PpsI GAGTC 3 cut(s) 278, 431, 673
PpuMI RGGWCCY 2 cut(s) 731, 1073
PscI ACATGT 1 cut(s) 56
Psp5II RGGWCCY 2 cut(s) 731, 1073
PspEI GGTNACC 1 cut(s) 361
PspN4I GGNNCC 5 cut(s) 1026, 1064, 1074, 1134, 1481
PspPI GGNCC 5 cut(s) 62, 731, 1024, 1073, 1448
PspPPI RGGWCCY 2 cut(s) 731, 1073
PsuI RGATCY 1 cut(s) 289
PvuII CAGCTG 2 cut(s) 473, 686
RsaI GTAC 2 cut(s) 376, 526
RsaNI GTAC 2 cut(s) 375, 525
RseI CAYNNNNRTG 2 cut(s) 34, 1488
SaqAI TTAA 1 cut(s) 411
SatI GCNGC 6 cut(s) 27, 236, 474, 723, 750, 985
Sau3AI GATC 7 cut(s) 85, 289, 621, 906, 1039, 1183, 1429
Sau96I GGNCC 5 cut(s) 62, 731, 1024, 1073, 1448
SchI GAGTC 3 cut(s) 278, 431, 673
SduI GDGCHC 1 cut(s) 1135
SfaNI GCATC 5 cut(s) 31, 145, 510, 823, 1209
SfcI CTRYAG 1 cut(s) 343
SinI GGWCC 4 cut(s) 62, 731, 1073, 1448
SmiMI CAYNNNNRTG 2 cut(s) 34, 1488
SmlI CTYRAG 4 cut(s) 149, 264, 1031, 1496
SmoI CTYRAG 4 cut(s) 149, 264, 1031, 1496
SpeI ACTAGT 1 cut(s) 277
SphI GCATGC 1 cut(s) 20
Sse9I AATT 8 cut(s) 165, 480, 645, 775, 862, 894, 1273, 1573
SsiI CCGC 6 cut(s) 535, 722, 985, 1230, 1446, 1466
SspMI CTAG 4 cut(s) 278, 323, 885, 1433
StyI CCWWGG 2 cut(s) 75, 1067
TaaI ACNGT 2 cut(s) 255, 329
TaqI TCGA 3 cut(s) 369, 1186, 1341
TasI AATT 8 cut(s) 165, 480, 645, 775, 862, 894, 1273, 1573
TatI WGTACW 1 cut(s) 374
TauI GCSGC 2 cut(s) 725, 987
TfiI GAWTC 4 cut(s) 161, 575, 994, 1508
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 3 cut(s) 260, 1161, 1290
TseFI GTSAC 1 cut(s) 441
TseI GCWGC 4 cut(s) 26, 235, 473, 749
Tsp45I GTSAC 1 cut(s) 441
TspGWI ACGGA 1 cut(s) 950
TspRI CASTG 3 cut(s) 260, 1161, 1290
Van91I CCANNNNNTGG 1 cut(s) 668
VpaK11BI GGWCC 4 cut(s) 62, 731, 1073, 1448
XapI RAATTY 2 cut(s) 480, 1273
XbaI TCTAGA 1 cut(s) 322
XceI RCATGY 2 cut(s) 20, 60
XspI CTAG 4 cut(s) 278, 323, 885, 1433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.