Rh2AG384300

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000338
Physical Location & Seq
Forward (+)
57399596 .. 57411936
12341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG384300.1

Sequence Viewer

Length: 2676 bp
ATGTGTTTTTACATTGCTAGGTTGAAATTTGGAGAAGTACAAGCGAAGCTTAGAGCTTACAGAGAAATGGATGGAAGTACTTCTGGTGTAGAAGACAATGATATTGATTGGAACACAGAAGATGAGCTTGAATTGGAAAACTTCACTTTATCTCCTTGTTCTAGTTTGCTACTTACTGGTGGCGAAGCTAATGCCGTCTCTGGGGAGGCGAGCTCATGTCCAAGTTCCTCCAACTCCAAAGTTCTTGATCAGTTTATAGGGATGGGATTTTCAAAGAAGATGGTCGAAAAAGCAATTCAGGAACATGGAGAAGAAAATACAGATGCAATTCTGGAAATGCTCCTCACAGATTCAGTTCTAGAAAGTTCTTCCCAAGAACTGCAACAAACCGATTCAGATCACTTTTCCTCTGATTATGATGGAAGCTTTTTGGATGACTTTTCAGATTTCGAAAGCTCTGGTGATGAGGAAATCATGAGTCCTACATCCGAGGAGGAAAAGCTACTCATGTCCTTGATCAAAATGGGGTACACAGAAGAGGAGGCTTCCATAGCTATAGAAAGATGTGGTCTTAGCTCTTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCATTTGGCAAAGGCAGAAGATGCCCTTCTTCCTCTAGAAGAGAAGCCTGGACTAAAACATTTGCACACTGATTATCCTAAGAAGAAAAGGAAACTTTTGGTTGATGAAGTGTCGAAAAGGAAAAGGAAAAATAGGCTCATAGGCGAGGATAATGAGACAGTTCGTCTCCCAAATCCAATGATTGGGTTTGGTATTCCAACTGGACCATGCCAATCTTACAGAAATCTTCCAAAGGCAGCCATAGGACCTCCCTATTTTTATTATGAAAATGTAGCCCTCACTCCTAAAGGGGTTTGGGACACTATATCGAGATTCCTATATGATGTTCCACCTGAATTTGTTGACTCTAAGTACTTCTGTGCTGCTGCTAGAAAAAGAGGTTATGTTCACAATCTTCCAATCGATAATAGAATCCCTCTCGTTCCACTTCCCCCACAAACCATACATGAGGCATTGCCCATGTCAAGAAAGTGGTGGCCTTCTTGGGACACACGGACAAAGCTAAATTGCTTACAAACTTGCCATTCAAGTGCAAAGTTAACAGAGAGGATCATGAAGGCTCTTGAGGGCTATGATGACATTCCACCAGAGAGAGTCCAGCGGGGTGTAATGCATGAATGCCGTAAGTGGAATCTAGTGTGGGTTGGAAAAAATAGGGTCGCCCCACTTGAGCCTGATGAATTTGAAATGCTTTTGGGGTTTCCAAAGGACCACACCAGGGGAATAAGCAGGACTGATAGATATAAATCACTCGGGAATGCATTTCAGGTGAGCTCAGCTCCAACTTCTTCCAACACCAAAGTTCTTGATCAGTTTATAGGGATGGGATTTTCTAAGAAGATGGTTGCCAATGCCATTCAGAAACATGGAGAAGAAAATACAGATGCAATTTTGGAAATGCTCCTCACAAATTCGGCTCTAGAAAGTTCAACTCATGAACAGAAACAAATGGATTCTGATCACTTCTCAGATTATGTGGAAAGCTTTCTAGATGGTTTTTCAGATATGGATAGTTGTGATGATGAGGAAATCATAAGTCATACATCTGAGGAGGAAAAACTAATGTCCTTGATCACAATGGGGTACACAGAAGAGGAGGCTTCCATGGCAGTAGAAAGATGTGGTCATAACTCCTCACTTCTGGAATTGACTGATTTTTTATCTGCTGCTCAATTGGCAAAGGAAGAAGATGCCCTTCTTCCTCTTGAAGAGAAGCCCAGACTAAAACATGTGCACACTGATCCTAAGAAGAAGAGGAAAATTATTGTATCTGATATATCGAAAAGGAAAAGGCAGATGGGGCACATAGACACAGATACTGAGACACTTTGCCTCCCAAATCCAATGATTGGGTTTGGGATTCCAACTGGAGCATGGAAATCTAACAGAATTCTTCCAAAGGCAGCCATAGGGCCTCCATATTTTTATTATGAGAATGTCGCCCTTACTCCTAAAGGGGTTTGGGACACTATATCCAAATTCCTATATGATGTTCCCCCTGAGTTTGTTGATTCAAAACATTTCTGTTCTGCTGCTAGAAAAAGGGGTTACGTCCATAATCTTCCTACTGAGAACAGATTTCCTCTTGTTCCGCTCCCCCCACAAACCATACATGAGGCATTTCCCTTGACAAAAAAATGGTGGCCTTCTTGGGACATAAGAACAAAGCTAAACTGTATAGTAACTTGCTATTCTAGTGCAAAGTTATCAGAGAGGATAATGAAGCTTCTCGAGGGCTGTGATGGCATTCCACCAGAAAGAGTCCAGCGGGCTGTTATACATGAATGCCGTAAGTGGAATCTATTGTGGGTTGGAAGAAGTAGGGTTGCCCCACTTGAGCCTGATGAAGTGGAAATGCTCATGGGGTTTCCAAAGGATCACACAAGGGGAATGAGCCGCACTGATAGATACAAATCGCTTGGCAATGCATTTCAGCTAATTGCTTCAGACATGGCTTTACTTGGTTTCTTTCATAACCCTATAGCATATGCAGCCAATGTGTTGGAGACAAAACATGAGTGGAAAGAGTTTGTTTACATACACATGCATGGAATTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

891

Amino Acids

100.97

Weight (kDa)

5.45

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 797, 1964
AccBSI CCGCTC 1 cut(s) 2209
AciI CCGC 4 cut(s) 1216, 2207, 2383, 2512
AclWI GGATC 3 cut(s) 1172, 1850, 2499
AcsI RAATTY 7 cut(s) 26, 950, 1295, 1525, 2004, 2093, 2667
AcuI CTGAAG 1 cut(s) 2544
AfaI GTAC 5 cut(s) 39, 79, 530, 968, 1700
AfiI CCNNNNNNNGG 4 cut(s) 201, 797, 1333, 1964
AflIII ACRYGT 1 cut(s) 1843
AgsI TTSAA 8 cut(s) 25, 131, 273, 1143, 1301, 1545, 1823, 2130
AhdI GACNNNNNGTC 1 cut(s) 777
AjnI CCWGG 2 cut(s) 661, 1331
Alw21I GWGCWC 3 cut(s) 215, 1391, 1851
Alw26I GTCTC 5 cut(s) 202, 764, 785, 1931, 2615
Alw44I GTGCAC 1 cut(s) 1847
AlwI GGATC 3 cut(s) 1172, 1850, 2499
AlwNI CAGNNNCTG 1 cut(s) 1934
Ama87I CYCGRG 2 cut(s) 1367, 2345
AoxI GGCC 3 cut(s) 1091, 2027, 2258
ApaLI GTGCAC 1 cut(s) 1847
ApeKI GCWGC 8 cut(s) 611, 851, 977, 980, 1781, 2018, 2147, 2606
ApoI RAATTY 7 cut(s) 26, 950, 1295, 1525, 2004, 2093, 2667
Asp700I GAANNNNTTC 2 cut(s) 79, 1599
AspS9I GGNCC 4 cut(s) 818, 860, 1324, 2027
AsuHPI GGTGA 2 cut(s) 473, 1396
AsuII TTCGAA 1 cut(s) 450
AvaI CYCGRG 2 cut(s) 1367, 2345
AvaII GGWCC 3 cut(s) 818, 860, 1324
BaeGI GKGCMC 2 cut(s) 1851, 1920
BanII GRGCYC 2 cut(s) 215, 1391
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 3 cut(s) 215, 1391, 1851
BbvI GCAGC 8 cut(s) 598, 863, 964, 967, 1768, 2030, 2134, 2618
BccI CCATC 9 cut(s) 65, 256, 274, 413, 1432, 1450, 1601, 1906, 2351
BceAI ACGGC 3 cut(s) 179, 1221, 2388
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 2 cut(s) 663, 1333
BclI TGATCA 5 cut(s) 247, 516, 1423, 1573, 1686
BcoDI GTCTC 5 cut(s) 202, 764, 785, 1931, 2615
BfmI CTRYAG 2 cut(s) 555, 2595
BisI GCNGC 9 cut(s) 612, 852, 978, 981, 1782, 2019, 2148, 2512, 2607
BlpI GCTNAGC 1 cut(s) 1390
BlsI GCNGC 9 cut(s) 613, 853, 979, 982, 1783, 2020, 2149, 2513, 2608
BmcAI AGTACT 2 cut(s) 79, 968
Bme1390I CCNGG 2 cut(s) 663, 1333
Bme18I GGWCC 3 cut(s) 818, 860, 1324
BmeRI GACNNNNNGTC 1 cut(s) 777
BmeT110I CYCGRG 2 cut(s) 1367, 2345
BmgT120I GGNCC 4 cut(s) 818, 860, 1324, 2027
BmrFI CCNGG 2 cut(s) 663, 1333
BmsI GCATC 4 cut(s) 313, 625, 1489, 1795
BpiI GAAGAC 1 cut(s) 99
BplI GAGNNNNNCTC 4 cut(s) 197, 229, 1378, 1410
BpmI CTGGAG 1 cut(s) 2004
Bpu1102I GCTNAGC 1 cut(s) 1390
Bpu14I TTCGAA 1 cut(s) 450
BpuEI CTTGAG 3 cut(s) 1199, 1304, 2471
Bsa29I ATCGAT 1 cut(s) 1017
BsaBI GATNNNNATC 4 cut(s) 396, 1360, 1572, 2527
BsaJI CCNNGG 3 cut(s) 489, 1332, 1719
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 1932, 1962
Bsc4I CCNNNNNNNGG 4 cut(s) 201, 797, 1333, 1964
Bse1I ACTGG 3 cut(s) 181, 820, 1987
Bse3DI GCAATG 3 cut(s) 12, 1067, 2545
Bse8I GATNNNNATC 4 cut(s) 396, 1360, 1572, 2527
BseBI CCWGG 2 cut(s) 663, 1333
BseCI ATCGAT 1 cut(s) 1017
BseDI CCNNGG 3 cut(s) 489, 1332, 1719
BseGI GGATG 5 cut(s) 76, 267, 439, 485, 1443
BseJI GATNNNNATC 4 cut(s) 396, 1360, 1572, 2527
BseLI CCNNNNNNNGG 4 cut(s) 201, 797, 1333, 1964
BseMI GCAATG 3 cut(s) 12, 1067, 2545
BseMII CTCAG 6 cut(s) 1404, 1596, 1653, 1926, 2106, 2175
BseNI ACTGG 3 cut(s) 181, 820, 1987
BseRI GAGGAG 7 cut(s) 332, 506, 554, 1508, 1679, 1724, 1738
BseSI GKGCMC 2 cut(s) 1851, 1920
BseXI GCAGC 8 cut(s) 598, 863, 964, 967, 1768, 2030, 2134, 2618
BshFI GGCC 3 cut(s) 1093, 2029, 2260
BshVI ATCGAT 1 cut(s) 1017
BsiHKAI GWGCWC 3 cut(s) 215, 1391, 1851
BsiHKCI CYCGRG 2 cut(s) 1367, 2345
BslFI GGGAC 4 cut(s) 926, 1115, 2093, 2282
BslI CCNNNNNNNGG 4 cut(s) 201, 797, 1333, 1964
BsmAI GTCTC 5 cut(s) 202, 764, 785, 1931, 2615
BsmBI CGTCTC 2 cut(s) 202, 785
BsmFI GGGAC 4 cut(s) 926, 1115, 2093, 2282
BsmI GAATGC 4 cut(s) 1238, 1378, 2361, 2405
BsnI GGCC 3 cut(s) 1093, 2029, 2260
BsoBI CYCGRG 2 cut(s) 1367, 2345
Bsp119I TTCGAA 1 cut(s) 450
Bsp1286I GDGCHC 4 cut(s) 215, 1391, 1851, 1920
Bsp143I GATC 9 cut(s) 247, 397, 516, 1164, 1423, 1573, 1686, 1855, 2491
Bsp1720I GCTNAGC 1 cut(s) 1390
Bsp19I CCATGG 1 cut(s) 1719
BspACI CCGC 4 cut(s) 1216, 2207, 2383, 2512
BspANI GGCC 3 cut(s) 1093, 2029, 2260
BspCNI CTCAG 6 cut(s) 1403, 1595, 1654, 1927, 2107, 2176
BspDI ATCGAT 1 cut(s) 1017
BspHI TCATGA 3 cut(s) 474, 1167, 1549
BspPI GGATC 3 cut(s) 1172, 1850, 2499
BspQI GCTCTTC 1 cut(s) 583
BspT104I TTCGAA 1 cut(s) 450
BsrBI CCGCTC 1 cut(s) 2209
BsrDI GCAATG 3 cut(s) 12, 1067, 2545
BsrI ACTGG 3 cut(s) 181, 820, 1987
BssECI CCNNGG 3 cut(s) 489, 1332, 1719
BssMI GATC 9 cut(s) 247, 397, 516, 1164, 1423, 1573, 1686, 1855, 2491
BssT1I CCWWGG 1 cut(s) 1719
Bst2UI CCWGG 2 cut(s) 663, 1333
Bst4CI ACNGT 2 cut(s) 775, 2291
Bst6I CTCTTC 6 cut(s) 531, 583, 648, 1701, 1818, 1862
BstAPI GCANNNNNTGC 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 450
BstC8I GCNNGC 2 cut(s) 211, 2385
BstDSI CCRYGG 1 cut(s) 1719
BstF5I GGATG 5 cut(s) 76, 267, 439, 485, 1443
BstKTI GATC 9 cut(s) 250, 400, 519, 1167, 1426, 1576, 1689, 1858, 2494
BstMAI GTCTC 5 cut(s) 202, 764, 785, 1931, 2615
BstMBI GATC 9 cut(s) 247, 397, 516, 1164, 1423, 1573, 1686, 1855, 2491
BstMWI GCNNNNNNNGC 8 cut(s) 551, 620, 635, 1721, 1790, 1915, 2358, 2606
BstNI CCWGG 2 cut(s) 663, 1333
BstNSI RCATGY 2 cut(s) 1847, 2662
BstSCI CCNGG 2 cut(s) 661, 1331
BstSFI CTRYAG 2 cut(s) 555, 2595
BstSLI GKGCMC 2 cut(s) 1851, 1920
BstV1I GCAGC 8 cut(s) 598, 863, 964, 967, 1768, 2030, 2134, 2618
BstV2I GAAGAC 1 cut(s) 99
BstXI CCANNNNNNTGG 1 cut(s) 2617
Bsu15I ATCGAT 1 cut(s) 1017
BsuRI GGCC 3 cut(s) 1093, 2029, 2260
BsuTUI ATCGAT 1 cut(s) 1017
BtgI CCRYGG 1 cut(s) 1719
BtsCI GGATG 5 cut(s) 76, 267, 439, 485, 1443
BtsIMutI CAGTG 3 cut(s) 681, 1851, 2514
Cac8I GCNNGC 2 cut(s) 211, 2385
CaiI CAGNNNCTG 1 cut(s) 1934
CciI TCATGA 3 cut(s) 474, 1167, 1549
Cfr13I GGNCC 4 cut(s) 818, 860, 1324, 2027
ClaI ATCGAT 1 cut(s) 1017
Csp6I GTAC 5 cut(s) 38, 78, 529, 967, 1699
CviQI GTAC 5 cut(s) 38, 78, 529, 967, 1699
DpnI GATC 9 cut(s) 249, 399, 518, 1166, 1425, 1575, 1688, 1857, 2493
DpnII GATC 9 cut(s) 247, 397, 516, 1164, 1423, 1573, 1686, 1855, 2491
DriI GACNNNNNGTC 1 cut(s) 777
Eam1104I CTCTTC 6 cut(s) 531, 583, 648, 1701, 1818, 1862
Eam1105I GACNNNNNGTC 1 cut(s) 777
EarI CTCTTC 6 cut(s) 531, 583, 648, 1701, 1818, 1862
Ecl136II GAGCTC 2 cut(s) 213, 1389
Eco130I CCWWGG 1 cut(s) 1719
Eco24I GRGCYC 2 cut(s) 215, 1391
Eco47I GGWCC 3 cut(s) 818, 860, 1324
Eco53kI GAGCTC 2 cut(s) 213, 1389
Eco57I CTGAAG 1 cut(s) 2544
Eco88I CYCGRG 2 cut(s) 1367, 2345
EcoICRI GAGCTC 2 cut(s) 213, 1389
EcoO109I RGGNCCY 2 cut(s) 860, 2027
EcoRI GAATTC 2 cut(s) 2004, 2667
EcoRII CCWGG 2 cut(s) 661, 1331
EcoT14I CCWWGG 1 cut(s) 1719
EcoT22I ATGCAT 4 cut(s) 1230, 1378, 2545, 2664
EcoT38I GRGCYC 2 cut(s) 215, 1391
ErhI CCWWGG 1 cut(s) 1719
Esp3I CGTCTC 2 cut(s) 202, 785
FalI AAGNNNNNCTT 8 cut(s) 33, 65, 111, 143, 624, 656, 1794, 1826
FaqI GGGAC 4 cut(s) 926, 1115, 2093, 2282
FauI CCCGC 2 cut(s) 1209, 2376
FauNDI CATATG 1 cut(s) 2602
FbaI TGATCA 5 cut(s) 247, 516, 1423, 1573, 1686
Fnu4HI GCNGC 9 cut(s) 612, 852, 978, 981, 1782, 2019, 2148, 2512, 2607
FokI GGATG 5 cut(s) 83, 274, 446, 472, 1450
FriOI GRGCYC 2 cut(s) 215, 1391
Fsp4HI GCNGC 9 cut(s) 612, 852, 978, 981, 1782, 2019, 2148, 2512, 2607
GluI GCNGC 9 cut(s) 612, 852, 978, 981, 1782, 2019, 2148, 2512, 2607
GsuI CTGGAG 1 cut(s) 2004
HaeIII GGCC 3 cut(s) 1093, 2029, 2260
HincII GTYRAC 2 cut(s) 958, 1155
HindII GTYRAC 2 cut(s) 958, 1155
HindIII AAGCTT 4 cut(s) 47, 424, 1597, 2339
HpaI GTTAAC 1 cut(s) 1155
HphI GGTGA 2 cut(s) 473, 1396
Hpy166II GTNNAC 7 cut(s) 531, 958, 1003, 1155, 1701, 1849, 2650
Hpy8I GTNNAC 7 cut(s) 531, 958, 1003, 1155, 1701, 1849, 2650
HpyAV CCTTC 5 cut(s) 650, 1104, 1165, 1820, 2271
HpyCH4III ACNGT 2 cut(s) 775, 2291
HpyCH4IV ACGT 1 cut(s) 2166
HpyF10VI GCNNNNNNNGC 8 cut(s) 551, 620, 635, 1721, 1790, 1915, 2358, 2606
HpySE526I ACGT 1 cut(s) 2166
Ksp22I TGATCA 5 cut(s) 247, 516, 1423, 1573, 1686
KspAI GTTAAC 1 cut(s) 1155
Kzo9I GATC 9 cut(s) 247, 397, 516, 1164, 1423, 1573, 1686, 1855, 2491
LguI GCTCTTC 1 cut(s) 583
LmnI GCTCC 5 cut(s) 345, 1399, 1521, 1985, 2214
Lsp1109I GCAGC 8 cut(s) 598, 863, 964, 967, 1768, 2030, 2134, 2618
LweI GCATC 4 cut(s) 313, 625, 1489, 1795
MaeII ACGT 1 cut(s) 2166
MaeIII GTNAC 2 cut(s) 2162, 2296
MalI GATC 9 cut(s) 249, 399, 518, 1166, 1425, 1575, 1688, 1857, 2493
MbiI CCGCTC 1 cut(s) 2209
MboI GATC 9 cut(s) 247, 397, 516, 1164, 1423, 1573, 1686, 1855, 2491
MfeI CAATTG 1 cut(s) 1787
MhlI GDGCHC 4 cut(s) 215, 1391, 1851, 1920
MlyI GAGTC 4 cut(s) 487, 953, 1218, 2385
MmeI TCCRAC 8 cut(s) 255, 836, 1240, 1421, 1431, 2003, 2407, 2598
Mph1103I ATGCAT 4 cut(s) 1230, 1378, 2545, 2664
MroXI GAANNNNTTC 2 cut(s) 79, 1599
MseI TTAA 1 cut(s) 1154
MslI CAYNNNNRTG 3 cut(s) 1143, 2657, 2661
MspA1I CMGCKG 2 cut(s) 1216, 2383
MspR9I CCNGG 2 cut(s) 663, 1333
MunI CAATTG 1 cut(s) 1787
Mva1269I GAATGC 4 cut(s) 1238, 1378, 2361, 2405
MvaI CCWGG 2 cut(s) 663, 1333
MwoI GCNNNNNNNGC 8 cut(s) 551, 620, 635, 1721, 1790, 1915, 2358, 2606
NcoI CCATGG 1 cut(s) 1719
NdeI CATATG 1 cut(s) 2602
NdeII GATC 9 cut(s) 247, 397, 516, 1164, 1423, 1573, 1686, 1855, 2491
NsiI ATGCAT 4 cut(s) 1230, 1378, 2545, 2664
NspI RCATGY 2 cut(s) 1847, 2662
NspV TTCGAA 1 cut(s) 450
PaeR7I CTCGAG 1 cut(s) 2345
PagI TCATGA 3 cut(s) 474, 1167, 1549
PciI ACATGT 1 cut(s) 1843
PciSI GCTCTTC 1 cut(s) 583
PctI GAATGC 4 cut(s) 1238, 1378, 2361, 2405
PdmI GAANNNNTTC 2 cut(s) 79, 1599
PfeI GAWTC 9 cut(s) 350, 392, 927, 1026, 1246, 1568, 1975, 2126, 2413
PflMI CCANNNNNTGG 2 cut(s) 797, 1964
PkrI GCNGC 9 cut(s) 613, 853, 979, 982, 1783, 2020, 2149, 2513, 2608
PleI GAGTC 4 cut(s) 486, 953, 1217, 2384
PpsI GAGTC 4 cut(s) 486, 953, 1217, 2384
PpuMI RGGWCCY 1 cut(s) 860
PscI ACATGT 1 cut(s) 1843
Psp124BI GAGCTC 2 cut(s) 215, 1391
Psp5II RGGWCCY 1 cut(s) 860
Psp6I CCWGG 2 cut(s) 661, 1331
PspGI CCWGG 2 cut(s) 661, 1331
PspPI GGNCC 4 cut(s) 818, 860, 1324, 2027
PspPPI RGGWCCY 1 cut(s) 860
PstNI CAGNNNCTG 1 cut(s) 1934
RsaI GTAC 5 cut(s) 39, 79, 530, 968, 1700
RsaNI GTAC 5 cut(s) 38, 78, 529, 967, 1699
RseI CAYNNNNRTG 3 cut(s) 1143, 2657, 2661
SacI GAGCTC 2 cut(s) 215, 1391
SapI GCTCTTC 1 cut(s) 583
SaqAI TTAA 1 cut(s) 1154
SatI GCNGC 9 cut(s) 612, 852, 978, 981, 1782, 2019, 2148, 2512, 2607
Sau3AI GATC 9 cut(s) 247, 397, 516, 1164, 1423, 1573, 1686, 1855, 2491
Sau96I GGNCC 4 cut(s) 818, 860, 1324, 2027
ScaI AGTACT 2 cut(s) 79, 968
SchI GAGTC 4 cut(s) 487, 953, 1218, 2385
ScrFI CCNGG 2 cut(s) 663, 1333
SduI GDGCHC 4 cut(s) 215, 1391, 1851, 1920
SfaNI GCATC 4 cut(s) 313, 625, 1489, 1795
SfcI CTRYAG 2 cut(s) 555, 2595
Sfr274I CTCGAG 1 cut(s) 2345
SfuI TTCGAA 1 cut(s) 450
SinI GGWCC 3 cut(s) 818, 860, 1324
SlaI CTCGAG 1 cut(s) 2345
SmiMI CAYNNNNRTG 3 cut(s) 1143, 2657, 2661
SmlI CTYRAG 4 cut(s) 1178, 1283, 2345, 2450
SmoI CTYRAG 4 cut(s) 1178, 1283, 2345, 2450
SsiI CCGC 4 cut(s) 1216, 2207, 2383, 2512
SstI GAGCTC 2 cut(s) 215, 1391
StyD4I CCNGG 2 cut(s) 661, 1331
StyI CCWWGG 1 cut(s) 1719
TaaI ACNGT 2 cut(s) 775, 2291
TaiI ACGT 1 cut(s) 2169
TaqI TCGA 7 cut(s) 285, 450, 728, 923, 1017, 1895, 2346
TatI WGTACW 3 cut(s) 37, 77, 966
TauI GCSGC 1 cut(s) 2514
TfiI GAWTC 9 cut(s) 350, 392, 927, 1026, 1246, 1568, 1975, 2126, 2413
Tru1I TTAA 1 cut(s) 1154
Tru9I TTAA 1 cut(s) 1154
TscAI CASTG 3 cut(s) 688, 1858, 2521
TseI GCWGC 8 cut(s) 611, 851, 977, 980, 1781, 2018, 2147, 2606
TspGWI ACGGA 1 cut(s) 1123
TspRI CASTG 3 cut(s) 688, 1858, 2521
Van91I CCANNNNNTGG 2 cut(s) 797, 1964
VneI GTGCAC 1 cut(s) 1847
VpaK11BI GGWCC 3 cut(s) 818, 860, 1324
XapI RAATTY 7 cut(s) 26, 950, 1295, 1525, 2004, 2093, 2667
XbaI TCTAGA 4 cut(s) 358, 649, 1534, 1603
XceI RCATGY 2 cut(s) 1847, 2662
XcmI CCANNNNNNNNNTGG 1 cut(s) 1986
XhoI CTCGAG 1 cut(s) 2345
XmnI GAANNNNTTC 2 cut(s) 79, 1599
ZrmI AGTACT 2 cut(s) 79, 968
Zsp2I ATGCAT 4 cut(s) 1230, 1378, 2545, 2664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.