RchiOBHm_Chr1g0322511

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000338
Physical Location & Seq
Reverse (-)
9874800 .. 9880971
6172 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55250

Sequence Viewer

Length: 1056 bp
ATGGATGGAAATACATCTCGGGTAGATGGTGATGATGTTGACTGGGATACTGATGATGAGCTTGAGATTGAAAACTTCACTTTATCTTCTTCAGCTAATCCAGGCTCTTGGGAGTCCTCCGGAGCAACATTCTCTGCCAGAGATTCTCCTCCAGAGCAGTGCTCTCCTCAAGAACAGCAACAAACTGACTCGGATCTCTTTTCTTCAGATTATGATGGGAGCTTTCTAGATAGTTTTTCAGACCTCGTTAGCTCTGATGACGAGGTAACCATCGATCCTACATCTGAGGCGGAGACAAAACTTATGTCCCTGGTTAAAATGAGGTACAATGAATTGGAGGCTTCAATGGCAATAGAAAGATGCGGTATTGACTCGTTGCTTGTTGATTTGACTGATTTCATATCAGCTGCTCAAATTTCAAAGGCAGAAGGTGGCCATCTTCCCCTTGAAGAGATGTGTTCACATCTGCGAAGTGAGTATGCTGAGAAGAATAAAAGGAAACTTTGTGAATCTTCATTGCTGAAAAGGAAAAGGATTATGGGACATGGAAACCAGATCATAGGGGAAGATGCTGGTGCAGTTCATCTCCCAAATCCAATAAATGGGTTTGGGACTCCAGCTGATCTCTGTCAAATCCAGAGAAGTCTTCCAGAGGCAGCCACAGGACCTCCTTATTTTTACTATGAGAATGTGGCATTGACTCCTAAAGGAGTTTGGAGCACAATATCCCGCTTTTTGTATGACGTGAAGCCTGAGTTTGTTGGTTCAAAGTATTTTTGTGCTGCTGCAAGAAAGAGGGGTTATATGCACAATCTTCCAATCAAAAACAGAATTCCTCTCGTTCCCCTTCTACCACAAACTATTTCTGATGCATTTCCGATGACAAGGAGGTGGTGGCCTACTTGGGATGATCGGACAAAGCTAAATTGCTTACAGACTTGCATTGCTAGTGCAACAATTACTGACAGGATCATGAAGGCTCTTAAAGACTATAACGAAGATGAAATCCCACCAGAAAGTGTCCGTCGCTATGTTCTTTATGAATGCCGCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

351

Amino Acids

39.44

Weight (kDa)

4.69

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 602
AccIII TCCGGA 1 cut(s) 119
AciI CCGC 4 cut(s) 290, 363, 730, 1048
AclWI GGATC 3 cut(s) 201, 269, 977
AcoI YGGCCR 1 cut(s) 433
AcsI RAATTY 2 cut(s) 414, 831
AcuI CTGAAG 2 cut(s) 75, 189
AfaI GTAC 1 cut(s) 326
AfiI CCNNNNNNNGG 1 cut(s) 602
AgsI TTSAA 5 cut(s) 71, 345, 420, 449, 768
AjiI CACGTC 1 cut(s) 745
AjnI CCWGG 2 cut(s) 100, 309
AluBI AGCT 7 cut(s) 61, 95, 222, 252, 407, 620, 922
AluI AGCT 7 cut(s) 61, 95, 222, 252, 407, 620, 922
Alw21I GWGCWC 2 cut(s) 164, 722
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 3 cut(s) 201, 269, 977
Ama87I CYCGRG 1 cut(s) 18
Aor13HI TCCGGA 1 cut(s) 119
AoxI GGCC 2 cut(s) 433, 896
ApeKI GCWGC 4 cut(s) 407, 656, 782, 785
ApoI RAATTY 2 cut(s) 414, 831
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AspS9I GGNCC 1 cut(s) 665
AsuHPI GGTGA 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 18
AvaII GGWCC 1 cut(s) 665
BalI TGGCCA 1 cut(s) 435
BbsI GAAGAC 1 cut(s) 638
Bbv12I GWGCWC 2 cut(s) 164, 722
BbvI GCAGC 4 cut(s) 394, 668, 769, 772
BccI CCATC 4 cut(s) 20, 209, 278, 444
BciT130I CCWGG 2 cut(s) 102, 311
BciVI GTATCC 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 2 cut(s) 227, 948
BfuI GTATCC 1 cut(s) 40
BisI GCNGC 5 cut(s) 408, 657, 783, 786, 1048
BlsI GCNGC 5 cut(s) 409, 658, 784, 787, 1049
Bme1390I CCNGG 2 cut(s) 102, 311
Bme18I GGWCC 1 cut(s) 665
BmeT110I CYCGRG 1 cut(s) 18
BmgBI CACGTC 1 cut(s) 745
BmgT120I GGNCC 1 cut(s) 665
BmrFI CCNGG 2 cut(s) 102, 311
BmrI ACTGGG 1 cut(s) 52
BmsI GCATC 3 cut(s) 350, 559, 859
BmuI ACTGGG 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 638
BplI GAGNNNNNCTC 2 cut(s) 146, 178
BpmI CTGGAG 2 cut(s) 135, 600
BpuEI CTTGAG 2 cut(s) 83, 153
Bsa29I ATCGAT 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 309
BsaWI WCCGGW 1 cut(s) 119
BsaXI ACNNNNNCTCC 2 cut(s) 652, 682
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse1I ACTGG 1 cut(s) 47
Bse3DI GCAATG 2 cut(s) 515, 942
BseAI TCCGGA 1 cut(s) 119
BseBI CCWGG 2 cut(s) 102, 311
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 2 cut(s) 10, 913
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMI GCAATG 2 cut(s) 515, 942
BseMII CTCAG 3 cut(s) 276, 474, 744
BseNI ACTGG 1 cut(s) 47
BseRI GAGGAG 2 cut(s) 138, 156
BseXI GCAGC 4 cut(s) 394, 668, 769, 772
BsgI GTGCAG 1 cut(s) 597
BshFI GGCC 2 cut(s) 435, 898
BshVI ATCGAT 1 cut(s) 273
BsiHKAI GWGCWC 2 cut(s) 164, 722
BsiHKCI CYCGRG 1 cut(s) 18
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 3 cut(s) 292, 555, 625
BslI CCNNNNNNNGG 1 cut(s) 602
BsmAI GTCTC 1 cut(s) 287
BsmFI GGGAC 3 cut(s) 292, 555, 625
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 2 cut(s) 435, 898
BsoBI CYCGRG 1 cut(s) 18
Bsp1286I GDGCHC 2 cut(s) 164, 722
Bsp13I TCCGGA 1 cut(s) 119
Bsp143I GATC 6 cut(s) 193, 274, 555, 622, 910, 969
BspACI CCGC 4 cut(s) 290, 363, 730, 1048
BspANI GGCC 2 cut(s) 435, 898
BspCNI CTCAG 3 cut(s) 277, 475, 745
BspDI ATCGAT 1 cut(s) 273
BspEI TCCGGA 1 cut(s) 119
BspHI TCATGA 1 cut(s) 972
BspPI GGATC 3 cut(s) 201, 269, 977
BsrDI GCAATG 2 cut(s) 515, 942
BsrI ACTGG 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 6 cut(s) 193, 274, 555, 622, 910, 969
Bst2UI CCWGG 2 cut(s) 102, 311
Bst6I CTCTTC 1 cut(s) 444
BstDEI CTNAG 3 cut(s) 285, 483, 753
BstEII GGTNACC 1 cut(s) 265
BstF5I GGATG 2 cut(s) 10, 913
BstKTI GATC 6 cut(s) 196, 277, 558, 625, 913, 972
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 6 cut(s) 193, 274, 555, 622, 910, 969
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNI CCWGG 2 cut(s) 102, 311
BstPI GGTNACC 1 cut(s) 265
BstSCI CCNGG 2 cut(s) 100, 309
BstV1I GCAGC 4 cut(s) 394, 668, 769, 772
BstV2I GAAGAC 1 cut(s) 638
BstX2I RGATCY 1 cut(s) 193
BstXI CCANNNNNNTGG 1 cut(s) 108
BstYI RGATCY 1 cut(s) 193
Bsu15I ATCGAT 1 cut(s) 273
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 2 cut(s) 435, 898
BsuTUI ATCGAT 1 cut(s) 273
BtrI CACGTC 1 cut(s) 745
BtsCI GGATG 2 cut(s) 10, 913
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 1 cut(s) 164
CciI TCATGA 1 cut(s) 972
Cfr13I GGNCC 1 cut(s) 665
ClaI ATCGAT 1 cut(s) 273
Csp6I GTAC 1 cut(s) 325
CviAII CATG 2 cut(s) 545, 973
CviQI GTAC 1 cut(s) 325
DdeI CTNAG 3 cut(s) 285, 483, 753
DpnI GATC 6 cut(s) 195, 276, 557, 624, 912, 971
DpnII GATC 6 cut(s) 193, 274, 555, 622, 910, 969
EaeI YGGCCR 1 cut(s) 433
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
EciI GGCGGA 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 665
Eco57I CTGAAG 2 cut(s) 75, 189
Eco88I CYCGRG 1 cut(s) 18
Eco91I GGTNACC 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 665
EcoO65I GGTNACC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 831
EcoRII CCWGG 2 cut(s) 100, 309
EcoT22I ATGCAT 1 cut(s) 874
FaeI CATG 2 cut(s) 548, 976
FaqI GGGAC 3 cut(s) 292, 555, 625
FatI CATG 2 cut(s) 544, 972
FauI CCCGC 1 cut(s) 737
Fnu4HI GCNGC 5 cut(s) 408, 657, 783, 786, 1048
FokI GGATG 2 cut(s) 17, 920
Fsp4HI GCNGC 5 cut(s) 408, 657, 783, 786, 1048
FspBI CTAG 2 cut(s) 227, 948
GluI GCNGC 5 cut(s) 408, 657, 783, 786, 1048
GsuI CTGGAG 2 cut(s) 135, 600
HaeIII GGCC 2 cut(s) 435, 898
HapII CCGG 1 cut(s) 120
Hin1II CATG 2 cut(s) 548, 976
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HinfI GANTC 7 cut(s) 113, 143, 188, 371, 509, 613, 700
HpaII CCGG 1 cut(s) 120
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 2 cut(s) 40, 461
Hpy188I TCNGA 8 cut(s) 193, 208, 241, 256, 286, 868, 879, 915
Hpy188III TCNNGA 7 cut(s) 120, 152, 170, 227, 637, 650, 973
Hpy8I GTNNAC 2 cut(s) 40, 461
Hpy99I CGWCG 1 cut(s) 1029
HpyAV CCTTC 3 cut(s) 422, 857, 970
HpyCH4IV ACGT 1 cut(s) 744
HpyCH4V TGCA 6 cut(s) 578, 788, 808, 872, 942, 953
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 3 cut(s) 285, 483, 753
HpySE526I ACGT 1 cut(s) 744
Hsp92II CATG 2 cut(s) 548, 976
Kpn2I TCCGGA 1 cut(s) 119
Kzo9I GATC 6 cut(s) 193, 274, 555, 622, 910, 969
LmnI GCTCC 3 cut(s) 122, 219, 717
Lsp1109I GCAGC 4 cut(s) 394, 668, 769, 772
LweI GCATC 3 cut(s) 350, 559, 859
MaeI CTAG 2 cut(s) 227, 948
MaeII ACGT 1 cut(s) 744
MaeIII GTNAC 1 cut(s) 265
MalI GATC 6 cut(s) 195, 276, 557, 624, 912, 971
MboI GATC 6 cut(s) 193, 274, 555, 622, 910, 969
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 2 cut(s) 164, 722
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 5 cut(s) 332, 414, 831, 925, 957
MluNI TGGCCA 1 cut(s) 435
MlyI GAGTC 5 cut(s) 122, 182, 365, 607, 694
Mox20I TGGCCA 1 cut(s) 435
Mph1103I ATGCAT 1 cut(s) 874
MroI TCCGGA 1 cut(s) 119
MscI TGGCCA 1 cut(s) 435
MseI TTAA 2 cut(s) 315, 984
Msp20I TGGCCA 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 407, 620
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 2 cut(s) 102, 311
Mva1269I GAATGC 1 cut(s) 1049
MvaI CCWGG 2 cut(s) 102, 311
MwoI GCNNNNNNNGC 1 cut(s) 347
NdeII GATC 6 cut(s) 193, 274, 555, 622, 910, 969
NlaIII CATG 2 cut(s) 548, 976
NsiI ATGCAT 1 cut(s) 874
PagI TCATGA 1 cut(s) 972
PctI GAATGC 1 cut(s) 1049
PfeI GAWTC 2 cut(s) 143, 509
PflMI CCANNNNNTGG 1 cut(s) 602
PkrI GCNGC 5 cut(s) 409, 658, 784, 787, 1049
PleI GAGTC 5 cut(s) 121, 182, 365, 607, 694
PpsI GAGTC 5 cut(s) 121, 182, 365, 607, 694
PpuMI RGGWCCY 1 cut(s) 665
Psp5II RGGWCCY 1 cut(s) 665
Psp6I CCWGG 2 cut(s) 100, 309
PspEI GGTNACC 1 cut(s) 265
PspGI CCWGG 2 cut(s) 100, 309
PspPI GGNCC 1 cut(s) 665
PspPPI RGGWCCY 1 cut(s) 665
PsuI RGATCY 1 cut(s) 193
PvuII CAGCTG 2 cut(s) 407, 620
RsaI GTAC 1 cut(s) 326
RsaNI GTAC 1 cut(s) 325
SaqAI TTAA 2 cut(s) 315, 984
SatI GCNGC 5 cut(s) 408, 657, 783, 786, 1048
Sau3AI GATC 6 cut(s) 193, 274, 555, 622, 910, 969
Sau96I GGNCC 1 cut(s) 665
SchI GAGTC 5 cut(s) 122, 182, 365, 607, 694
ScrFI CCNGG 2 cut(s) 102, 311
SduI GDGCHC 2 cut(s) 164, 722
SfaNI GCATC 3 cut(s) 350, 559, 859
SinI GGWCC 1 cut(s) 665
SmlI CTYRAG 2 cut(s) 62, 168
SmoI CTYRAG 2 cut(s) 62, 168
Sse9I AATT 5 cut(s) 332, 414, 831, 925, 957
SsiI CCGC 4 cut(s) 290, 363, 730, 1048
SspMI CTAG 2 cut(s) 227, 948
StyD4I CCNGG 2 cut(s) 100, 309
TaiI ACGT 1 cut(s) 747
TaqI TCGA 1 cut(s) 273
TasI AATT 5 cut(s) 332, 414, 831, 925, 957
TauI GCSGC 1 cut(s) 1050
TfiI GAWTC 2 cut(s) 143, 509
Tru1I TTAA 2 cut(s) 315, 984
Tru9I TTAA 2 cut(s) 315, 984
TscAI CASTG 1 cut(s) 164
TseI GCWGC 4 cut(s) 407, 656, 782, 785
TspDTI ATGAA 7 cut(s) 345, 388, 504, 572, 989, 1017, 1056
TspGWI ACGGA 1 cut(s) 1013
TspRI CASTG 1 cut(s) 164
Van91I CCANNNNNTGG 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 665
XapI RAATTY 2 cut(s) 414, 831
XbaI TCTAGA 1 cut(s) 226
XspI CTAG 2 cut(s) 227, 948
Zsp2I ATGCAT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.