Rmu_sc0023751.1_g000001

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0023751.1
OG0000338
Physical Location & Seq
Forward (+)
1 .. 2206
2206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0023751.1_g000001.1.cds

Sequence Viewer

Length: 1292 bp
aattggaggcttcaatcgcaatagaaagatgtggtgtcgactcatcccttgttgatttgactgatttcatatcagctgctcagttttcaagggcagaagatgcccattttccccttgaagagaagcctagacgtcttcacattgatgacaagaagaaaaggaaactttgtgaatatgccatgtcgaaaagaaaaaagaaggtggggcttggaaatgggatcattgaggaagatgatgaggcacttcatctcccaaatccaatgattggattcggaactccaacacatctttgtcaaatccacaggagtattccagaggcagccacaggacctccttatttctactatgaaaatgtggcattgactcctaaaggggtttggaacacaatatcccgatttctctatgatatccagcctgaatttgttgattccaagtacttctgtgcggctgctaggaaaagaggttatgttcataatctcccaatagagaacaggtttcctctcattccccttccaccgcagactatttttgatgcatttcccatgacaaggagatggtggcctagttgggacgagcgcagacagctgaattgcctacagacttgcattgctagtgcaaagttgactgagaggatcaggctggctcttaaggattacgatgaagatgagattccaccagaaagagtccgacggtatgttctatatgaatgccgcaagtggaatcttgtgtgggtaggaaagaacaaggttgccccattggagcctgatgaggtggaaacacttttggggtttccgaggaaccatacaaggggaataagtaggaccgatagatacaagtcactagggaattcattccagattgatacagtagcctatcatctctctgtgttgaaagatcggttccccaatggcatcaatctcctctctcttttctctggaattggcggtgcagagatagccctccatcggcttggaattcgaatgaagaatgttgtgtctgttgagatttcagcggtgagcagaactgttgtgaggacttggtgggagcaaaccaaccaaaagggaaacttgtatcaccttgctgatgtgcaggagttgaatgctgaccggttggagcattacatcaatacctttggtgggtttgatctcgtggttggtgggagcccgtgcaacaatcttgccggtagcaacagacatcatagggatgggcttgagggaaaagaatcttctctattttatgattatttccgtatattagacttagtgaagggtcttatggcaagacatagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

429

Amino Acids

49.22

Weight (kDa)

8.5

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccB7I CCANNNNNTGG 1 cut(s) 265
AccI GTMKAC 1 cut(s) 38
AciI CCGC 5 cut(s) 445, 517, 711, 944, 1012
AclWI GGATC 2 cut(s) 226, 640
AcsI RAATTY 3 cut(s) 418, 846, 974
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 5 cut(s) 265, 548, 807, 965, 1136
AflII CTTAAG 1 cut(s) 646
AgeI ACCGGT 1 cut(s) 1106
AgsI TTSAA 5 cut(s) 14, 89, 118, 891, 1098
AloI GAACNNNNNNTCC 2 cut(s) 373, 405
AluBI AGCT 3 cut(s) 76, 585, 1289
AluI AGCT 3 cut(s) 76, 585, 1289
AlwI GGATC 2 cut(s) 226, 640
AoxI GGCC 1 cut(s) 559
ApeKI GCWGC 3 cut(s) 76, 319, 448
ApoI RAATTY 3 cut(s) 418, 846, 974
AsiGI ACCGGT 1 cut(s) 1106
Asp700I GAANNNNTTC 1 cut(s) 850
AspLEI GCGC 1 cut(s) 578
AspS9I GGNCC 2 cut(s) 328, 821
AsuHPI GGTGA 2 cut(s) 1026, 1066
AsuII TTCGAA 1 cut(s) 978
AvaII GGWCC 2 cut(s) 328, 821
BanII GRGCYC 1 cut(s) 1165
BauI CACGAG 1 cut(s) 1147
BbsI GAAGAC 1 cut(s) 127
BbvI GCAGC 3 cut(s) 63, 331, 435
BccI CCATC 3 cut(s) 548, 971, 1198
BfaI CTAG 5 cut(s) 128, 452, 563, 611, 841
BfmI CTRYAG 1 cut(s) 595
BfrI CTTAAG 1 cut(s) 646
BisI GCNGC 5 cut(s) 77, 320, 446, 449, 711
BlsI GCNGC 5 cut(s) 78, 321, 447, 450, 712
BmcAI AGTACT 1 cut(s) 436
Bme18I GGWCC 2 cut(s) 328, 821
BmgT120I GGNCC 2 cut(s) 328, 821
BmiI GGNNCC 4 cut(s) 761, 799, 901, 1162
BmsI GCATC 3 cut(s) 90, 522, 920
BpiI GAAGAC 1 cut(s) 127
Bpu14I TTCGAA 1 cut(s) 978
BpuEI CTTGAG 1 cut(s) 1231
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 1 cut(s) 793
BsaWI WCCGGW 1 cut(s) 1106
BsaXI ACNNNNNCTCC 4 cut(s) 233, 263, 315, 345
Bsc4I CCNNNNNNNGG 5 cut(s) 265, 548, 807, 965, 1136
Bse118I RCCGGY 2 cut(s) 1106, 1180
Bse3DI GCAATG 1 cut(s) 605
BseDI CCNNGG 1 cut(s) 793
BseGI GGATG 2 cut(s) 43, 1209
BseLI CCNNNNNNNGG 5 cut(s) 265, 548, 807, 965, 1136
BseMI GCAATG 1 cut(s) 605
BseMII CTCAG 2 cut(s) 94, 617
BseRI GAGGAG 1 cut(s) 910
BseXI GCAGC 3 cut(s) 63, 331, 435
BsgI GTGCAG 2 cut(s) 968, 1108
BshFI GGCC 1 cut(s) 561
BshTI ACCGGT 1 cut(s) 1106
BsiSI CCGG 2 cut(s) 1107, 1181
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 5 cut(s) 265, 548, 807, 965, 1136
BsmFI GGGAC 1 cut(s) 583
BsmI GAATGC 2 cut(s) 712, 1104
BsnI GGCC 1 cut(s) 561
Bsp119I TTCGAA 1 cut(s) 978
Bsp1286I GDGCHC 1 cut(s) 1165
Bsp143I GATC 4 cut(s) 218, 632, 894, 1143
BspACI CCGC 5 cut(s) 445, 517, 711, 944, 1012
BspANI GGCC 1 cut(s) 561
BspCNI CTCAG 2 cut(s) 93, 618
BspLI GGNNCC 4 cut(s) 761, 799, 901, 1162
BspPI GGATC 2 cut(s) 226, 640
BspT104I TTCGAA 1 cut(s) 978
BspTI CTTAAG 1 cut(s) 646
BsrDI GCAATG 1 cut(s) 605
BsrFI RCCGGY 2 cut(s) 1106, 1180
BssAI RCCGGY 2 cut(s) 1106, 1180
BssECI CCNNGG 1 cut(s) 793
BssMI GATC 4 cut(s) 218, 632, 894, 1143
BssNI GRCGYC 1 cut(s) 132
BssSI CACGAG 1 cut(s) 1147
Bst2BI CACGAG 1 cut(s) 1147
Bst4CI ACNGT 3 cut(s) 692, 867, 1026
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 132
BstAFI CTTAAG 1 cut(s) 646
BstAPI GCANNNNNTGC 1 cut(s) 100
BstBI TTCGAA 1 cut(s) 978
BstC8I GCNNGC 1 cut(s) 641
BstDEI CTNAG 3 cut(s) 80, 626, 1260
BstF5I GGATG 2 cut(s) 43, 1209
BstHHI GCGC 1 cut(s) 578
BstKTI GATC 4 cut(s) 221, 635, 897, 1146
BstMBI GATC 4 cut(s) 218, 632, 894, 1143
BstMWI GCNNNNNNNGC 4 cut(s) 16, 100, 582, 955
BstSFI CTRYAG 1 cut(s) 595
BstV1I GCAGC 3 cut(s) 63, 331, 435
BstV2I GAAGAC 1 cut(s) 127
BstXI CCANNNNNNTGG 1 cut(s) 970
BsuRI GGCC 1 cut(s) 561
BtsCI GGATG 2 cut(s) 43, 1209
Cac8I GCNNGC 1 cut(s) 641
CfoI GCGC 1 cut(s) 578
Cfr10I RCCGGY 2 cut(s) 1106, 1180
Cfr13I GGNCC 2 cut(s) 328, 821
Csp6I GTAC 1 cut(s) 435
CspAI ACCGGT 1 cut(s) 1106
CviAII CATG 2 cut(s) 180, 543
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 3 cut(s) 80, 626, 1260
DpnI GATC 4 cut(s) 220, 634, 896, 1145
DpnII GATC 4 cut(s) 218, 632, 894, 1143
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 1165
Eco32I GATATC 1 cut(s) 408
Eco47I GGWCC 2 cut(s) 328, 821
EcoO109I RGGNCCY 1 cut(s) 328
EcoRI GAATTC 2 cut(s) 846, 974
EcoRV GATATC 1 cut(s) 408
EcoT22I ATGCAT 1 cut(s) 537
EcoT38I GRGCYC 1 cut(s) 1165
FaeI CATG 2 cut(s) 183, 546
FalI AAGNNNNNCTT 2 cut(s) 1051, 1083
FaqI GGGAC 1 cut(s) 583
FatI CATG 2 cut(s) 179, 542
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 5 cut(s) 77, 320, 446, 449, 711
FokI GGATG 2 cut(s) 30, 1216
FriOI GRGCYC 1 cut(s) 1165
Fsp4HI GCNGC 5 cut(s) 77, 320, 446, 449, 711
FspBI CTAG 5 cut(s) 128, 452, 563, 611, 841
GlaI GCGC 1 cut(s) 577
GluI GCNGC 5 cut(s) 77, 320, 446, 449, 711
HaeIII GGCC 1 cut(s) 561
HapII CCGG 2 cut(s) 1107, 1181
HhaI GCGC 1 cut(s) 578
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 2 cut(s) 183, 546
Hin6I GCGC 1 cut(s) 576
HinP1I GCGC 1 cut(s) 576
HincII GTYRAC 2 cut(s) 39, 623
HindII GTYRAC 2 cut(s) 39, 623
HinfI GANTC 8 cut(s) 40, 269, 363, 427, 669, 683, 720, 1222
HpaII CCGG 2 cut(s) 1107, 1181
HphI GGTGA 2 cut(s) 1026, 1066
Hpy166II GTNNAC 2 cut(s) 39, 623
Hpy188I TCNGA 3 cut(s) 274, 688, 794
Hpy188III TCNNGA 4 cut(s) 313, 392, 855, 935
Hpy8I GTNNAC 2 cut(s) 39, 623
Hpy99I CGWCG 1 cut(s) 692
HpyAV CCTTC 3 cut(s) 192, 520, 1260
HpyCH4III ACNGT 3 cut(s) 692, 867, 1026
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 6 cut(s) 535, 605, 616, 949, 1089, 1169
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 100, 582, 955
HpyF3I CTNAG 3 cut(s) 80, 626, 1260
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 2 cut(s) 183, 546
HspAI GCGC 1 cut(s) 576
Kzo9I GATC 4 cut(s) 218, 632, 894, 1143
LmnI GCTCC 4 cut(s) 759, 1044, 1113, 1160
Lsp1109I GCAGC 3 cut(s) 63, 331, 435
LweI GCATC 3 cut(s) 90, 522, 920
MaeI CTAG 5 cut(s) 128, 452, 563, 611, 841
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 836
MalI GATC 4 cut(s) 220, 634, 896, 1145
MboI GATC 4 cut(s) 218, 632, 894, 1143
MboII GAAGA 8 cut(s) 109, 127, 130, 165, 241, 673, 996, 1217
MhlI GDGCHC 1 cut(s) 1165
MluCI AATT 5 cut(s) 418, 588, 846, 938, 974
MlyI GAGTC 3 cut(s) 34, 357, 692
MmeI TCCRAC 3 cut(s) 304, 711, 1091
Mph1103I ATGCAT 1 cut(s) 537
MroXI GAANNNNTTC 1 cut(s) 850
MseI TTAA 1 cut(s) 647
MslI CAYNNNNRTG 2 cut(s) 143, 1202
MspA1I CMGCKG 3 cut(s) 76, 585, 1012
MspCI CTTAAG 1 cut(s) 646
MspI CCGG 2 cut(s) 1107, 1181
Mva1269I GAATGC 2 cut(s) 712, 1104
MwoI GCNNNNNNNGC 4 cut(s) 16, 100, 582, 955
NdeII GATC 4 cut(s) 218, 632, 894, 1143
NlaIII CATG 2 cut(s) 183, 546
NlaIV GGNNCC 4 cut(s) 761, 799, 901, 1162
NmuCI GTSAC 1 cut(s) 836
NsiI ATGCAT 1 cut(s) 537
NspV TTCGAA 1 cut(s) 978
PctI GAATGC 2 cut(s) 712, 1104
PdmI GAANNNNTTC 1 cut(s) 850
PfeI GAWTC 5 cut(s) 269, 427, 669, 720, 1222
PflMI CCANNNNNTGG 1 cut(s) 265
PinAI ACCGGT 1 cut(s) 1106
PkrI GCNGC 5 cut(s) 78, 321, 447, 450, 712
PleI GAGTC 3 cut(s) 34, 357, 691
PpsI GAGTC 3 cut(s) 34, 357, 691
PpuMI RGGWCCY 1 cut(s) 328
Psp5II RGGWCCY 1 cut(s) 328
PspN4I GGNNCC 4 cut(s) 761, 799, 901, 1162
PspPI GGNCC 2 cut(s) 328, 821
PspPPI RGGWCCY 1 cut(s) 328
PvuII CAGCTG 2 cut(s) 76, 585
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 2 cut(s) 143, 1202
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 1 cut(s) 647
SatI GCNGC 5 cut(s) 77, 320, 446, 449, 711
Sau3AI GATC 4 cut(s) 218, 632, 894, 1143
Sau96I GGNCC 2 cut(s) 328, 821
ScaI AGTACT 1 cut(s) 436
SchI GAGTC 3 cut(s) 34, 357, 692
SduI GDGCHC 1 cut(s) 1165
SfaNI GCATC 3 cut(s) 90, 522, 920
SfcI CTRYAG 1 cut(s) 595
SfuI TTCGAA 1 cut(s) 978
SinI GGWCC 2 cut(s) 328, 821
SmiMI CAYNNNNRTG 2 cut(s) 143, 1202
SmlI CTYRAG 2 cut(s) 646, 1210
SmoI CTYRAG 2 cut(s) 646, 1210
Sse9I AATT 5 cut(s) 418, 588, 846, 938, 974
SsiI CCGC 5 cut(s) 445, 517, 711, 944, 1012
SspMI CTAG 5 cut(s) 128, 452, 563, 611, 841
TaaI ACNGT 3 cut(s) 692, 867, 1026
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 38, 184, 978
TaqII GACCGA 1 cut(s) 838
TasI AATT 5 cut(s) 418, 588, 846, 938, 974
TatI WGTACW 1 cut(s) 434
TauI GCSGC 2 cut(s) 448, 713
TfiI GAWTC 5 cut(s) 269, 427, 669, 720, 1222
Tru1I TTAA 1 cut(s) 647
Tru9I TTAA 1 cut(s) 647
TseFI GTSAC 1 cut(s) 836
TseI GCWGC 3 cut(s) 76, 319, 448
Tsp45I GTSAC 1 cut(s) 836
TspDTI ATGAA 8 cut(s) 57, 235, 362, 460, 674, 719, 839, 997
TspGWI ACGGA 1 cut(s) 1237
Van91I CCANNNNNTGG 1 cut(s) 265
Vha464I CTTAAG 1 cut(s) 646
VpaK11BI GGWCC 2 cut(s) 328, 821
XapI RAATTY 3 cut(s) 418, 846, 974
XmiI GTMKAC 1 cut(s) 38
XmnI GAANNNNTTC 1 cut(s) 850
XspI CTAG 5 cut(s) 128, 452, 563, 611, 841
ZraI GACGTC 1 cut(s) 133
ZrmI AGTACT 1 cut(s) 436
Zsp2I ATGCAT 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.