Rw2G052520

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000338
Physical Location & Seq
Forward (+)
80439919 .. 80446753
6835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G052520.1

Sequence Viewer

Length: 1929 bp
ATGCTACTAGATCAAATAATAATATTTTCTTTATCAAGTCAATATAAAGGAAACTTGGACATCTTCCAGAGCATATCTGCTGGGAAGCAATTTGGCATATTAATGTCTTCCTTTACCTTGAACACACTTTCGAAACAGTTGATAGCTTCCTGGATTGTTCCTATTGCTGGATTATTTATTTTCTACTGTTTGCATAAAGCAACAAACCTTTGCGCGGGTGACCATAACATTTTAGAGACATGGTACTTCTTTTCTGGGAGCTCATCTCCACGACCTTCCAATACCAAAGTTTTTGGTCATTTTGTCGGCATGGGATTTTCTCAAAAAATGGTTGCCAAGGCTATTCAGGAACATGGTGAGGAAGACGCCGATTCAATTCTGGAAAGCCTTCTCTTATATAGTGCTCTTGAAAATTCAACTCCAGAGCAGGATTCTCCACCAGAGCAGAGTTCTCCCCCAGAACAGTGCTTTCCTCCACAACAGCAACAAATCGATTCTGATCCCTTTTCTTCAGATTATGAGGGGAGCTTTCTGGATGGATTTTCAGATCTCGATAGCTCTGATAATGAGGAAATTAGCATTCCTACATCTGAGGAGGAGAAAAAACTTGTGGCCTTGGTCAAAATGGGGTACCCAGAATTGGAGGCTTCAATCGCAATAGAAAGATGTGGTGTCGACTCATCCCTTGTTGATTTGACTGATTTCATATCAGCTGCTCAGTTTTCAAGGGCAGAAGATGCCCATTTTCCCCTTGAAGAGAAGCCTAGACGTCTTCACATTGATGACAAGAAGAAAAGGAAACTTTGTGAATATGCCATGTCGAAAAGAAAAAAGAAGGTGGGGCTTGGAAATGGGATCATTGAGGAAGATGATGAGGCACTTCATCTCCCAAATCCAATGATTGGATTCGGAACTCCAACACATCTTTGTCAAATCCACAGGAGTATTCCAGAGGCAGCCACAGGACCTCCTTATTTCTACTATGAAAATGTGGCATTGACTCCTAAAGGGGTTTGGAACACAATATCCCGATTTCTCTATGATATCCAGCCTGAATTTGTTGATTCCAAGTACTTCTGTGCGGCTGCTAGGAAAAGAGGTTATGTTCATAATCTCCCAATAGAGAACAGGTTTCCTCTCATTCCCCTTCCACCGCAGACTATTTTTGATGCATTTCCCATGACAAGGAGATGGTGGCCTAGTTGGGACGAGCGCAGACAGCTGAATTGCCTACAGACTTGCATTGCTAGTGCAAAGTTGACTGAGAGGATCAGGCTGGCTCTTAAGGATTACGATGAAGATGAGATTCCACCAGAAAGAGTCCGACGGTATGTTCTATATGAATGCCGCAAGTGGAATCTTGTGTGGGTAGGAAAGAACAAGGTTGCCCCATTGGAGCCTGATGAGGTGGAAACACTTTTGGGGTTTCCGAGGAACCATACAAGGGGAATAAGTAGGACCGATAGATACAAGTCACTCGGGAATTCATTCCAGATTGATACAGTAGCCTATCATCTCTCTGTGTTGAAAGATCGGTTCCCCAATGGCATCAATCTCCTCTCTCTTTTCTCTGGAATTGGCGGTGCAGAGATAGCCCTCCATCGGCTTGGAATTCGAATGAAGAATGTTGTGTCTGTTGAGATTTCAGCGGTGAGCAGAACTGTTGTGAGGACTTGGTGGGAGCAAACCAACCAAAAGGGAAACTTGTATCACCTTGCTGATGTGCAGGAGTTGAATGCTGACCGGTTGGAGCATTACATCAATACCTTTGGTGGGTTTGATCTCGTGGTTGGTGGGAGCCCGTGCAACAATCTTGCCGGTAGCAACAGACATCATAGGGATGGGCTTGAGGGAAAAGAATCTTCTCTATTTTATGATTATTTCCGTATATTAGACTTAGTGAAGGGTCTTATGGCAAGACATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

642

Amino Acids

72.78

Weight (kDa)

5.76

Isoelectric Point (pI)

51.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 518 - 632 1.8e-09 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 772
Acc65I GGTACC 1 cut(s) 630
AccB1I GGYRCC 1 cut(s) 630
AccB7I CCANNNNNTGG 1 cut(s) 902
AccI GTMKAC 1 cut(s) 675
AccII CGCG 1 cut(s) 215
AciI CCGC 6 cut(s) 215, 1082, 1154, 1348, 1581, 1649
AclWI GGATC 3 cut(s) 494, 863, 1277
AcsI RAATTY 4 cut(s) 412, 1055, 1483, 1611
AcuI CTGAAG 1 cut(s) 495
AcyI GRCGYC 2 cut(s) 366, 769
AfaI GTAC 3 cut(s) 245, 632, 1073
AfiI CCNNNNNNNGG 8 cut(s) 167, 214, 640, 902, 1185, 1444, 1602, 1773
AflII CTTAAG 1 cut(s) 1283
AgeI ACCGGT 1 cut(s) 1743
AgsI TTSAA 9 cut(s) 121, 375, 410, 417, 651, 726, 755, 1528, 1735
AjnI CCWGG 1 cut(s) 149
AloI GAACNNNNNNTCC 2 cut(s) 1010, 1042
AluBI AGCT 7 cut(s) 146, 261, 528, 558, 713, 1222, 1926
AluI AGCT 7 cut(s) 146, 261, 528, 558, 713, 1222, 1926
Alw21I GWGCWC 2 cut(s) 263, 406
Alw26I GTCTC 1 cut(s) 230
AlwI GGATC 3 cut(s) 494, 863, 1277
Ama87I CYCGRG 1 cut(s) 1478
AoxI GGCC 2 cut(s) 612, 1196
ApeKI GCWGC 3 cut(s) 713, 956, 1085
ApoI RAATTY 4 cut(s) 412, 1055, 1483, 1611
AseI ATTAAT 1 cut(s) 101
AsiGI ACCGGT 1 cut(s) 1743
Asp700I GAANNNNTTC 2 cut(s) 387, 1487
Asp718I GGTACC 1 cut(s) 630
AspLEI GCGC 2 cut(s) 215, 1215
AspS9I GGNCC 2 cut(s) 965, 1458
AsuHPI GGTGA 4 cut(s) 230, 368, 1663, 1703
AsuII TTCGAA 2 cut(s) 131, 1615
AvaI CYCGRG 1 cut(s) 1478
AvaII GGWCC 2 cut(s) 965, 1458
BanI GGYRCC 1 cut(s) 630
BanII GRGCYC 2 cut(s) 263, 1802
BauI CACGAG 1 cut(s) 1784
BbsI GAAGAC 3 cut(s) 99, 369, 764
Bbv12I GWGCWC 2 cut(s) 263, 406
BbvI GCAGC 3 cut(s) 700, 968, 1072
BccI CCATC 4 cut(s) 530, 1185, 1608, 1835
BciT130I CCWGG 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 230
BfaI CTAG 5 cut(s) 8, 765, 1089, 1200, 1248
BfmI CTRYAG 1 cut(s) 1232
BfrI CTTAAG 1 cut(s) 1283
BglII AGATCT 1 cut(s) 547
BisI GCNGC 5 cut(s) 714, 957, 1083, 1086, 1348
BlsI GCNGC 5 cut(s) 715, 958, 1084, 1087, 1349
BmcAI AGTACT 1 cut(s) 1073
Bme1390I CCNGG 1 cut(s) 151
Bme18I GGWCC 2 cut(s) 965, 1458
BmeT110I CYCGRG 1 cut(s) 1478
BmgT120I GGNCC 2 cut(s) 965, 1458
BmiI GGNNCC 5 cut(s) 632, 1398, 1436, 1538, 1799
BmrFI CCNGG 1 cut(s) 151
BmsI GCATC 3 cut(s) 727, 1159, 1557
BpiI GAAGAC 3 cut(s) 99, 369, 764
BplI GAGNNNNNCTC 2 cut(s) 250, 282
BpmI CTGGAG 1 cut(s) 405
Bpu14I TTCGAA 2 cut(s) 131, 1615
BpuEI CTTGAG 1 cut(s) 1868
Bsa29I ATCGAT 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 498
BsaHI GRCGYC 2 cut(s) 366, 769
BsaJI CCNNGG 3 cut(s) 336, 615, 1430
BsaWI WCCGGW 1 cut(s) 1743
BsaXI ACNNNNNCTCC 4 cut(s) 870, 900, 952, 982
Bsc4I CCNNNNNNNGG 8 cut(s) 167, 214, 640, 902, 1185, 1444, 1602, 1773
Bse118I RCCGGY 2 cut(s) 1743, 1817
Bse3DI GCAATG 1 cut(s) 1242
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 1 cut(s) 151
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 3 cut(s) 336, 615, 1430
BseGI GGATG 3 cut(s) 541, 680, 1846
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 8 cut(s) 167, 214, 640, 902, 1185, 1444, 1602, 1773
BseMI GCAATG 1 cut(s) 1242
BseMII CTCAG 3 cut(s) 582, 731, 1254
BseRI GAGGAG 3 cut(s) 608, 611, 1547
BseXI GCAGC 3 cut(s) 700, 968, 1072
BseYI CCCAGC 1 cut(s) 80
BsgI GTGCAG 2 cut(s) 1605, 1745
Bsh1236I CGCG 1 cut(s) 215
BshFI GGCC 2 cut(s) 614, 1198
BshNI GGYRCC 1 cut(s) 630
BshTI ACCGGT 1 cut(s) 1743
BshVI ATCGAT 1 cut(s) 492
BsiHKAI GWGCWC 2 cut(s) 263, 406
BsiHKCI CYCGRG 1 cut(s) 1478
BsiSI CCGG 2 cut(s) 1744, 1818
BslFI GGGAC 1 cut(s) 1220
BslI CCNNNNNNNGG 8 cut(s) 167, 214, 640, 902, 1185, 1444, 1602, 1773
BsmAI GTCTC 1 cut(s) 230
BsmFI GGGAC 1 cut(s) 1220
BsmI GAATGC 3 cut(s) 579, 1349, 1741
BsnI GGCC 2 cut(s) 614, 1198
BsoBI CYCGRG 1 cut(s) 1478
Bsp119I TTCGAA 2 cut(s) 131, 1615
Bsp1286I GDGCHC 3 cut(s) 263, 406, 1802
Bsp143I GATC 7 cut(s) 10, 499, 547, 855, 1269, 1531, 1780
BspACI CCGC 6 cut(s) 215, 1082, 1154, 1348, 1581, 1649
BspANI GGCC 2 cut(s) 614, 1198
BspCNI CTCAG 3 cut(s) 583, 730, 1255
BspDI ATCGAT 1 cut(s) 492
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 5 cut(s) 632, 1398, 1436, 1538, 1799
BspPI GGATC 3 cut(s) 494, 863, 1277
BspT104I TTCGAA 2 cut(s) 131, 1615
BspT107I GGYRCC 1 cut(s) 630
BspTI CTTAAG 1 cut(s) 1283
BsrDI GCAATG 1 cut(s) 1242
BsrFI RCCGGY 2 cut(s) 1743, 1817
BssAI RCCGGY 2 cut(s) 1743, 1817
BssECI CCNNGG 3 cut(s) 336, 615, 1430
BssMI GATC 7 cut(s) 10, 499, 547, 855, 1269, 1531, 1780
BssNI GRCGYC 2 cut(s) 366, 769
BssSI CACGAG 1 cut(s) 1784
BssT1I CCWWGG 2 cut(s) 336, 615
Bst2BI CACGAG 1 cut(s) 1784
Bst2UI CCWGG 1 cut(s) 151
Bst4CI ACNGT 6 cut(s) 138, 188, 465, 1329, 1504, 1663
Bst6I CTCTTC 1 cut(s) 750
BstACI GRCGYC 2 cut(s) 366, 769
BstAFI CTTAAG 1 cut(s) 1283
BstAPI GCANNNNNTGC 1 cut(s) 737
BstBI TTCGAA 2 cut(s) 131, 1615
BstC8I GCNNGC 1 cut(s) 1278
BstDEI CTNAG 4 cut(s) 591, 717, 1263, 1897
BstEII GGTNACC 1 cut(s) 218
BstF5I GGATG 3 cut(s) 541, 680, 1846
BstFNI CGCG 1 cut(s) 215
BstHHI GCGC 2 cut(s) 215, 1215
BstKTI GATC 7 cut(s) 13, 502, 550, 858, 1272, 1534, 1783
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 7 cut(s) 10, 499, 547, 855, 1269, 1531, 1780
BstMWI GCNNNNNNNGC 4 cut(s) 653, 737, 1219, 1592
BstNI CCWGG 1 cut(s) 151
BstPI GGTNACC 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 149
BstSFI CTRYAG 1 cut(s) 1232
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 3 cut(s) 700, 968, 1072
BstV2I GAAGAC 3 cut(s) 99, 369, 764
BstX2I RGATCY 1 cut(s) 547
BstXI CCANNNNNNTGG 1 cut(s) 1607
BstYI RGATCY 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 492
BsuRI GGCC 2 cut(s) 614, 1198
BsuTUI ATCGAT 1 cut(s) 492
BtsCI GGATG 3 cut(s) 541, 680, 1846
BtsIMutI CAGTG 1 cut(s) 470
Cac8I GCNNGC 1 cut(s) 1278
CfoI GCGC 2 cut(s) 215, 1215
Cfr10I RCCGGY 2 cut(s) 1743, 1817
Cfr13I GGNCC 2 cut(s) 965, 1458
ClaI ATCGAT 1 cut(s) 492
CseI GACGC 1 cut(s) 374
Csp6I GTAC 3 cut(s) 244, 631, 1072
CspAI ACCGGT 1 cut(s) 1743
CviAII CATG 5 cut(s) 240, 310, 353, 817, 1180
CviQI GTAC 3 cut(s) 244, 631, 1072
DdeI CTNAG 4 cut(s) 591, 717, 1263, 1897
DpnI GATC 7 cut(s) 12, 501, 549, 857, 1271, 1533, 1782
DpnII GATC 7 cut(s) 10, 499, 547, 855, 1269, 1531, 1780
Eam1104I CTCTTC 1 cut(s) 750
EarI CTCTTC 1 cut(s) 750
Ecl136II GAGCTC 1 cut(s) 261
Eco130I CCWWGG 2 cut(s) 336, 615
Eco24I GRGCYC 2 cut(s) 263, 1802
Eco32I GATATC 1 cut(s) 1045
Eco47I GGWCC 2 cut(s) 965, 1458
Eco53kI GAGCTC 1 cut(s) 261
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 1 cut(s) 1478
Eco91I GGTNACC 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 965
EcoO65I GGTNACC 1 cut(s) 218
EcoRI GAATTC 2 cut(s) 1483, 1611
EcoRII CCWGG 1 cut(s) 149
EcoRV GATATC 1 cut(s) 1045
EcoT14I CCWWGG 2 cut(s) 336, 615
EcoT22I ATGCAT 1 cut(s) 1174
EcoT38I GRGCYC 2 cut(s) 263, 1802
ErhI CCWWGG 2 cut(s) 336, 615
FaeI CATG 5 cut(s) 243, 313, 356, 820, 1183
FalI AAGNNNNNCTT 2 cut(s) 1688, 1720
FaqI GGGAC 1 cut(s) 1220
FatI CATG 5 cut(s) 239, 309, 352, 816, 1179
FauI CCCGC 1 cut(s) 208
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 5 cut(s) 714, 957, 1083, 1086, 1348
FokI GGATG 3 cut(s) 548, 667, 1853
FriOI GRGCYC 2 cut(s) 263, 1802
Fsp4HI GCNGC 5 cut(s) 714, 957, 1083, 1086, 1348
FspBI CTAG 5 cut(s) 8, 765, 1089, 1200, 1248
GlaI GCGC 2 cut(s) 214, 1214
GluI GCNGC 5 cut(s) 714, 957, 1083, 1086, 1348
GsaI CCCAGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 405
HaeIII GGCC 2 cut(s) 614, 1198
HapII CCGG 2 cut(s) 1744, 1818
HgaI GACGC 1 cut(s) 374
HhaI GCGC 2 cut(s) 215, 1215
Hin1I GRCGYC 2 cut(s) 366, 769
Hin1II CATG 5 cut(s) 243, 313, 356, 820, 1183
Hin6I GCGC 2 cut(s) 213, 1213
HinP1I GCGC 2 cut(s) 213, 1213
HincII GTYRAC 2 cut(s) 676, 1260
HindII GTYRAC 2 cut(s) 676, 1260
HpaII CCGG 2 cut(s) 1744, 1818
HphI GGTGA 4 cut(s) 230, 368, 1663, 1703
Hpy166II GTNNAC 2 cut(s) 676, 1260
Hpy188I TCNGA 8 cut(s) 499, 514, 547, 562, 592, 911, 1325, 1431
Hpy8I GTNNAC 2 cut(s) 676, 1260
Hpy99I CGWCG 1 cut(s) 1329
HpyAV CCTTC 5 cut(s) 285, 398, 829, 1157, 1897
HpyCH4III ACNGT 6 cut(s) 138, 188, 465, 1329, 1504, 1663
HpyCH4IV ACGT 1 cut(s) 769
HpyCH4V TGCA 7 cut(s) 193, 1172, 1242, 1253, 1586, 1726, 1806
HpyF10VI GCNNNNNNNGC 4 cut(s) 653, 737, 1219, 1592
HpyF3I CTNAG 4 cut(s) 591, 717, 1263, 1897
HpySE526I ACGT 1 cut(s) 769
Hsp92I GRCGYC 2 cut(s) 366, 769
Hsp92II CATG 5 cut(s) 243, 313, 356, 820, 1183
HspAI GCGC 2 cut(s) 213, 1213
KpnI GGTACC 1 cut(s) 634
Kzo9I GATC 7 cut(s) 10, 499, 547, 855, 1269, 1531, 1780
LmnI GCTCC 6 cut(s) 258, 525, 1396, 1681, 1750, 1797
Lsp1109I GCAGC 3 cut(s) 700, 968, 1072
LweI GCATC 3 cut(s) 727, 1159, 1557
MaeI CTAG 5 cut(s) 8, 765, 1089, 1200, 1248
MaeII ACGT 1 cut(s) 769
MaeIII GTNAC 2 cut(s) 218, 1473
MalI GATC 7 cut(s) 12, 501, 549, 857, 1271, 1533, 1782
MboI GATC 7 cut(s) 10, 499, 547, 855, 1269, 1531, 1780
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 3 cut(s) 263, 406, 1802
MlyI GAGTC 3 cut(s) 671, 994, 1329
MmeI TCCRAC 3 cut(s) 941, 1348, 1728
Mph1103I ATGCAT 1 cut(s) 1174
MroXI GAANNNNTTC 2 cut(s) 387, 1487
MseI TTAA 2 cut(s) 101, 1284
MslI CAYNNNNRTG 3 cut(s) 101, 780, 1839
MspA1I CMGCKG 3 cut(s) 713, 1222, 1649
MspCI CTTAAG 1 cut(s) 1283
MspI CCGG 2 cut(s) 1744, 1818
MspR9I CCNGG 1 cut(s) 151
Mva1269I GAATGC 3 cut(s) 579, 1349, 1741
MvaI CCWGG 1 cut(s) 151
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 4 cut(s) 653, 737, 1219, 1592
NdeII GATC 7 cut(s) 10, 499, 547, 855, 1269, 1531, 1780
NlaIII CATG 5 cut(s) 243, 313, 356, 820, 1183
NlaIV GGNNCC 5 cut(s) 632, 1398, 1436, 1538, 1799
NmuCI GTSAC 2 cut(s) 218, 1473
NsiI ATGCAT 1 cut(s) 1174
NspV TTCGAA 2 cut(s) 131, 1615
PctI GAATGC 3 cut(s) 579, 1349, 1741
PdmI GAANNNNTTC 2 cut(s) 387, 1487
PfeI GAWTC 8 cut(s) 371, 431, 494, 906, 1064, 1306, 1357, 1859
PflMI CCANNNNNTGG 1 cut(s) 902
PfoI TCCNGGA 1 cut(s) 149
PinAI ACCGGT 1 cut(s) 1743
PkrI GCNGC 5 cut(s) 715, 958, 1084, 1087, 1349
PleI GAGTC 3 cut(s) 671, 994, 1328
PpsI GAGTC 3 cut(s) 671, 994, 1328
PpuMI RGGWCCY 1 cut(s) 965
PshBI ATTAAT 1 cut(s) 101
Psp124BI GAGCTC 1 cut(s) 263
Psp5II RGGWCCY 1 cut(s) 965
Psp6I CCWGG 1 cut(s) 149
PspEI GGTNACC 1 cut(s) 218
PspFI CCCAGC 1 cut(s) 80
PspGI CCWGG 1 cut(s) 149
PspN4I GGNNCC 5 cut(s) 632, 1398, 1436, 1538, 1799
PspPI GGNCC 2 cut(s) 965, 1458
PspPPI RGGWCCY 1 cut(s) 965
PsuI RGATCY 1 cut(s) 547
PvuII CAGCTG 2 cut(s) 713, 1222
RsaI GTAC 3 cut(s) 245, 632, 1073
RsaNI GTAC 3 cut(s) 244, 631, 1072
RseI CAYNNNNRTG 3 cut(s) 101, 780, 1839
SacI GAGCTC 1 cut(s) 263
SalI GTCGAC 1 cut(s) 674
SaqAI TTAA 2 cut(s) 101, 1284
SatI GCNGC 5 cut(s) 714, 957, 1083, 1086, 1348
Sau3AI GATC 7 cut(s) 10, 499, 547, 855, 1269, 1531, 1780
Sau96I GGNCC 2 cut(s) 965, 1458
ScaI AGTACT 1 cut(s) 1073
SchI GAGTC 3 cut(s) 671, 994, 1329
ScrFI CCNGG 1 cut(s) 151
SduI GDGCHC 3 cut(s) 263, 406, 1802
SfaNI GCATC 3 cut(s) 727, 1159, 1557
SfcI CTRYAG 1 cut(s) 1232
SfuI TTCGAA 2 cut(s) 131, 1615
SinI GGWCC 2 cut(s) 965, 1458
SmiMI CAYNNNNRTG 3 cut(s) 101, 780, 1839
SmlI CTYRAG 2 cut(s) 1283, 1847
SmoI CTYRAG 2 cut(s) 1283, 1847
SsiI CCGC 6 cut(s) 215, 1082, 1154, 1348, 1581, 1649
SspI AATATT 1 cut(s) 24
SspMI CTAG 5 cut(s) 8, 765, 1089, 1200, 1248
SstI GAGCTC 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 149
StyI CCWWGG 2 cut(s) 336, 615
TaaI ACNGT 6 cut(s) 138, 188, 465, 1329, 1504, 1663
TaiI ACGT 1 cut(s) 772
TaqI TCGA 6 cut(s) 131, 492, 552, 675, 821, 1615
TaqII GACCGA 1 cut(s) 1475
TatI WGTACW 1 cut(s) 1071
TauI GCSGC 2 cut(s) 1085, 1350
TfiI GAWTC 8 cut(s) 371, 431, 494, 906, 1064, 1306, 1357, 1859
Tru1I TTAA 2 cut(s) 101, 1284
Tru9I TTAA 2 cut(s) 101, 1284
TscAI CASTG 1 cut(s) 470
TseFI GTSAC 2 cut(s) 218, 1473
TseI GCWGC 3 cut(s) 713, 956, 1085
Tsp45I GTSAC 2 cut(s) 218, 1473
TspDTI ATGAA 8 cut(s) 694, 872, 999, 1097, 1311, 1356, 1476, 1634
TspGWI ACGGA 1 cut(s) 1874
TspRI CASTG 1 cut(s) 470
Van91I CCANNNNNTGG 1 cut(s) 902
Vha464I CTTAAG 1 cut(s) 1283
VpaK11BI GGWCC 2 cut(s) 965, 1458
VspI ATTAAT 1 cut(s) 101
XapI RAATTY 4 cut(s) 412, 1055, 1483, 1611
XmiI GTMKAC 1 cut(s) 675
XmnI GAANNNNTTC 2 cut(s) 387, 1487
XspI CTAG 5 cut(s) 8, 765, 1089, 1200, 1248
ZraI GACGTC 1 cut(s) 770
ZrmI AGTACT 1 cut(s) 1073
Zsp2I ATGCAT 1 cut(s) 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.