RchiOBHm_Chr1g0322691

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000338
Physical Location & Seq
Reverse (-)
10083074 .. 10084724
1651 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55267

Sequence Viewer

Length: 1113 bp
ATGCGGAGTGAGTATCCTGAGAAGAATAAAAGGAAACTTTGTGAATCTTCATTGCTGAAAAGGAAAAGGATTATGGGACATGGAAACCAGATCATAGGGGAAGATGCTGATGCAATTCATCTCCCAAATCCAATGATTGGGTTCCGGACTCTAGCTGATCTCTGTCAAATCCACAGAAGTCTTCCAGAGGCAGCCACAGGACCTCCTTATTTTTACTATGAGAATGTGGCATCGACTCCTAAAGGAGTTTGGAGCACAATATCCCGCTTTTTGTATGACATGAAGCCTGAGTTTGTTGATTCGAAATTTTTTTGTGCTGCTGCAAGAAAAAAGGGCTATGTTCACAATCTTCCAATTAAAAACAGATTTCCTCTCGTTCCCCTTCCACCACAAACTATATTTGGTGCATTTCCCATGACAAGGAGGTGGTGGCCTACTTGGGATGATCGGACAAAGCTAAATTGCTTACAGACTTGCATTGCTAGTGCAACAATTACTGAGAGGATCATGAAGGCTCTTAAAGACTATGACGAAGATGAAATCCCACCTGAAAGTGAAAGAAAAAAGGTGGCCCCACTTGAGCCTGATGAAGTAGAAATGCTTTTGGGGTTCCCAAGGGACCACACAAGGGGAATAAGCAGGACAGATAGATATAAGTCACTGGGAAATTCATTTCAGATTGATGCAGTGGCTTATGACCCGTTAGTTTTGAAAGATCGATTTCCCAATGGCATCAATGTCCTCTCTCTCTTCTCTGGGATTGGTGGTGCAGAGATAGCTCTTTACCGGCTTGGTATCCCGATGAAGAATGTTGTGTCAGTGGAGATTTTAGAGGTGAGCAGAAATGTTATGAGGTGTTGGTGGGAGCAGACAAACCAGAGAGGAAACTTGTATCACCTTGCTGATGTGCAAGATTTCAATGCTGACCGCTTAGAGCATTATATCAGTTCCTTTGGTGGGTTTGAACTGGTGATTGGTGGCAGTACATGTAACAATCTTGTGAGTAGCAACAGACACCATCGGGATGGGCTTAAGGGTAAAGAGTCTTCTCTTTTTTATGACTATTTTCGTATATTGGATTTGGTCAAGGGTATTATGGCAAGATACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

370

Amino Acids

42.41

Weight (kDa)

8.81

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 246 - 360 8.7e-06 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 137
AccIII TCCGGA 1 cut(s) 144
AciI CCGC 3 cut(s) 4, 265, 928
AclWI GGATC 1 cut(s) 512
AcsI RAATTY 2 cut(s) 305, 667
AfaI GTAC 1 cut(s) 985
AfiI CCNNNNNNNGG 4 cut(s) 137, 420, 628, 957
AflII CTTAAG 1 cut(s) 1031
AflIII ACRYGT 1 cut(s) 986
AgsI TTSAA 3 cut(s) 712, 919, 965
AjuI GAANNNNNNNTTGG 2 cut(s) 957, 989
AluBI AGCT 3 cut(s) 155, 457, 779
AluI AGCT 3 cut(s) 155, 457, 779
Alw21I GWGCWC 1 cut(s) 257
AlwI GGATC 1 cut(s) 512
Aor13HI TCCGGA 1 cut(s) 144
AoxI GGCC 2 cut(s) 431, 570
ApeKI GCWGC 3 cut(s) 191, 317, 320
ApoI RAATTY 2 cut(s) 305, 667
AspS9I GGNCC 3 cut(s) 200, 571, 619
AsuHPI GGTGA 3 cut(s) 847, 887, 982
AsuII TTCGAA 1 cut(s) 302
AvaII GGWCC 2 cut(s) 200, 619
BarI GAAGNNNNNNTAC 2 cut(s) 1030, 1062
BbsI GAAGAC 2 cut(s) 173, 1038
Bbv12I GWGCWC 1 cut(s) 257
BbvI GCAGC 3 cut(s) 203, 304, 307
BccI CCATC 2 cut(s) 1019, 1026
BciVI GTATCC 2 cut(s) 24, 806
BfaI CTAG 2 cut(s) 152, 483
BfrI CTTAAG 1 cut(s) 1031
BfuI GTATCC 2 cut(s) 24, 806
BisI GCNGC 3 cut(s) 192, 318, 321
BlsI GCNGC 3 cut(s) 193, 319, 322
Bme18I GGWCC 2 cut(s) 200, 619
BmgT120I GGNCC 3 cut(s) 200, 571, 619
BmiI GGNNCC 4 cut(s) 143, 573, 611, 620
BmrI ACTGGG 1 cut(s) 671
BmsI GCATC 5 cut(s) 94, 100, 239, 673, 741
BmuI ACTGGG 1 cut(s) 671
BpiI GAAGAC 2 cut(s) 173, 1038
Bpu14I TTCGAA 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 599
Bsa29I ATCGAT 1 cut(s) 718
BsaJI CCNNGG 1 cut(s) 614
BsaWI WCCGGW 1 cut(s) 144
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 420, 628, 957
Bse118I RCCGGY 1 cut(s) 786
Bse1I ACTGG 2 cut(s) 666, 972
Bse3DI GCAATG 2 cut(s) 50, 477
BseAI TCCGGA 1 cut(s) 144
BseCI ATCGAT 1 cut(s) 718
BseDI CCNNGG 1 cut(s) 614
BseGI GGATG 2 cut(s) 448, 1030
BseLI CCNNNNNNNGG 4 cut(s) 137, 420, 628, 957
BseMI GCAATG 2 cut(s) 50, 477
BseMII CTCAG 3 cut(s) 9, 279, 489
BseNI ACTGG 2 cut(s) 666, 972
BseXI GCAGC 3 cut(s) 203, 304, 307
BsgI GTGCAG 1 cut(s) 789
BshFI GGCC 2 cut(s) 433, 572
BshVI ATCGAT 1 cut(s) 718
BsiHKAI GWGCWC 1 cut(s) 257
BsiSI CCGG 2 cut(s) 145, 787
BslFI GGGAC 2 cut(s) 90, 632
BslI CCNNNNNNNGG 4 cut(s) 137, 420, 628, 957
BsmFI GGGAC 2 cut(s) 90, 632
BsnI GGCC 2 cut(s) 433, 572
Bsp119I TTCGAA 1 cut(s) 302
Bsp1286I GDGCHC 1 cut(s) 257
Bsp13I TCCGGA 1 cut(s) 144
Bsp143I GATC 5 cut(s) 90, 157, 445, 504, 715
BspACI CCGC 3 cut(s) 4, 265, 928
BspANI GGCC 2 cut(s) 433, 572
BspCNI CTCAG 3 cut(s) 10, 280, 490
BspDI ATCGAT 1 cut(s) 718
BspEI TCCGGA 1 cut(s) 144
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 4 cut(s) 143, 573, 611, 620
BspPI GGATC 1 cut(s) 512
BspT104I TTCGAA 1 cut(s) 302
BspTI CTTAAG 1 cut(s) 1031
BsrDI GCAATG 2 cut(s) 50, 477
BsrFI RCCGGY 1 cut(s) 786
BsrI ACTGG 2 cut(s) 666, 972
BssAI RCCGGY 1 cut(s) 786
BssECI CCNNGG 1 cut(s) 614
BssMI GATC 5 cut(s) 90, 157, 445, 504, 715
BssT1I CCWWGG 1 cut(s) 614
Bst6I CTCTTC 1 cut(s) 755
BstAFI CTTAAG 1 cut(s) 1031
BstBI TTCGAA 1 cut(s) 302
BstDEI CTNAG 4 cut(s) 18, 288, 498, 931
BstF5I GGATG 2 cut(s) 448, 1030
BstKTI GATC 5 cut(s) 93, 160, 448, 507, 718
BstMBI GATC 5 cut(s) 90, 157, 445, 504, 715
BstMWI GCNNNNNNNGC 1 cut(s) 776
BstNSI RCATGY 1 cut(s) 990
BstV1I GCAGC 3 cut(s) 203, 304, 307
BstV2I GAAGAC 2 cut(s) 173, 1038
BstXI CCANNNNNNTGG 1 cut(s) 1025
Bsu15I ATCGAT 1 cut(s) 718
BsuI GTATCC 2 cut(s) 24, 806
BsuRI GGCC 2 cut(s) 433, 572
BsuTUI ATCGAT 1 cut(s) 718
BtsCI GGATG 2 cut(s) 448, 1030
BtsI GCAGTG 1 cut(s) 693
BtsIMutI CAGTG 3 cut(s) 659, 693, 825
CciI TCATGA 1 cut(s) 507
Cfr10I RCCGGY 1 cut(s) 786
Cfr13I GGNCC 3 cut(s) 200, 571, 619
ClaI ATCGAT 1 cut(s) 718
Csp6I GTAC 1 cut(s) 984
CviAII CATG 5 cut(s) 80, 280, 415, 508, 987
CviQI GTAC 1 cut(s) 984
DdeI CTNAG 4 cut(s) 18, 288, 498, 931
DpnI GATC 5 cut(s) 92, 159, 447, 506, 717
DpnII GATC 5 cut(s) 90, 157, 445, 504, 715
Eam1104I CTCTTC 1 cut(s) 755
EarI CTCTTC 1 cut(s) 755
Eco130I CCWWGG 1 cut(s) 614
Eco47I GGWCC 2 cut(s) 200, 619
EcoO109I RGGNCCY 1 cut(s) 200
EcoT14I CCWWGG 1 cut(s) 614
ErhI CCWWGG 1 cut(s) 614
FaeI CATG 5 cut(s) 83, 283, 418, 511, 990
FaqI GGGAC 2 cut(s) 90, 632
FatI CATG 5 cut(s) 79, 279, 414, 507, 986
FauI CCCGC 1 cut(s) 272
Fnu4HI GCNGC 3 cut(s) 192, 318, 321
FokI GGATG 2 cut(s) 455, 1037
Fsp4HI GCNGC 3 cut(s) 192, 318, 321
FspBI CTAG 2 cut(s) 152, 483
GluI GCNGC 3 cut(s) 192, 318, 321
HaeIII GGCC 2 cut(s) 433, 572
HapII CCGG 2 cut(s) 145, 787
Hin1II CATG 5 cut(s) 83, 283, 418, 511, 990
HinfI GANTC 5 cut(s) 44, 148, 235, 299, 1043
HpaII CCGG 2 cut(s) 145, 787
HphI GGTGA 3 cut(s) 847, 887, 982
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 2 cut(s) 450, 678
Hpy188III TCNNGA 6 cut(s) 17, 145, 185, 508, 799, 1022
Hpy8I GTNNAC 1 cut(s) 343
HpyAV CCTTC 2 cut(s) 392, 505
HpyCH4V TGCA 8 cut(s) 113, 323, 407, 477, 488, 686, 770, 910
HpyF10VI GCNNNNNNNGC 1 cut(s) 776
HpyF3I CTNAG 4 cut(s) 18, 288, 498, 931
Hsp92II CATG 5 cut(s) 83, 283, 418, 511, 990
Kpn2I TCCGGA 1 cut(s) 144
Kzo9I GATC 5 cut(s) 90, 157, 445, 504, 715
LmnI GCTCC 2 cut(s) 252, 865
Lsp1109I GCAGC 3 cut(s) 203, 304, 307
LweI GCATC 5 cut(s) 94, 100, 239, 673, 741
MaeI CTAG 2 cut(s) 152, 483
MaeIII GTNAC 2 cut(s) 657, 989
MalI GATC 5 cut(s) 92, 159, 447, 506, 717
MboI GATC 5 cut(s) 90, 157, 445, 504, 715
MboII GAAGA 9 cut(s) 34, 39, 113, 173, 341, 545, 742, 817, 1038
MfeI CAATTG 1 cut(s) 1108
MhlI GDGCHC 1 cut(s) 257
MluCI AATT 7 cut(s) 114, 305, 354, 460, 492, 667, 1108
MlyI GAGTC 3 cut(s) 142, 229, 1052
MnlI CCTC 9 cut(s) 181, 213, 381, 417, 495, 752, 826, 846, 875
MroI TCCGGA 1 cut(s) 144
MseI TTAA 3 cut(s) 357, 519, 1032
MslI CAYNNNNRTG 1 cut(s) 1023
MspCI CTTAAG 1 cut(s) 1031
MspI CCGG 2 cut(s) 145, 787
MunI CAATTG 1 cut(s) 1108
MwoI GCNNNNNNNGC 1 cut(s) 776
NdeII GATC 5 cut(s) 90, 157, 445, 504, 715
NlaIII CATG 5 cut(s) 83, 283, 418, 511, 990
NlaIV GGNNCC 4 cut(s) 143, 573, 611, 620
NmuCI GTSAC 1 cut(s) 657
NspI RCATGY 1 cut(s) 990
NspV TTCGAA 1 cut(s) 302
PagI TCATGA 1 cut(s) 507
PciI ACATGT 1 cut(s) 986
PfeI GAWTC 2 cut(s) 44, 299
PflMI CCANNNNNTGG 1 cut(s) 137
PkrI GCNGC 3 cut(s) 193, 319, 322
PleI GAGTC 3 cut(s) 142, 229, 1051
PpsI GAGTC 3 cut(s) 142, 229, 1051
PpuMI RGGWCCY 1 cut(s) 200
PscI ACATGT 1 cut(s) 986
Psp5II RGGWCCY 1 cut(s) 200
PspN4I GGNNCC 4 cut(s) 143, 573, 611, 620
PspPI GGNCC 3 cut(s) 200, 571, 619
PspPPI RGGWCCY 1 cut(s) 200
RsaI GTAC 1 cut(s) 985
RsaNI GTAC 1 cut(s) 984
RseI CAYNNNNRTG 1 cut(s) 1023
SaqAI TTAA 3 cut(s) 357, 519, 1032
SatI GCNGC 3 cut(s) 192, 318, 321
Sau3AI GATC 5 cut(s) 90, 157, 445, 504, 715
Sau96I GGNCC 3 cut(s) 200, 571, 619
SchI GAGTC 3 cut(s) 142, 229, 1052
SduI GDGCHC 1 cut(s) 257
SfaNI GCATC 5 cut(s) 94, 100, 239, 673, 741
SfuI TTCGAA 1 cut(s) 302
SinI GGWCC 2 cut(s) 200, 619
SmiMI CAYNNNNRTG 1 cut(s) 1023
SmlI CTYRAG 2 cut(s) 578, 1031
SmoI CTYRAG 2 cut(s) 578, 1031
Sse9I AATT 7 cut(s) 114, 305, 354, 460, 492, 667, 1108
SsiI CCGC 3 cut(s) 4, 265, 928
SspMI CTAG 2 cut(s) 152, 483
StyI CCWWGG 1 cut(s) 614
TaqI TCGA 3 cut(s) 233, 302, 718
TasI AATT 7 cut(s) 114, 305, 354, 460, 492, 667, 1108
TatI WGTACW 1 cut(s) 983
TfiI GAWTC 2 cut(s) 44, 299
Tru1I TTAA 3 cut(s) 357, 519, 1032
Tru9I TTAA 3 cut(s) 357, 519, 1032
TscAI CASTG 3 cut(s) 666, 693, 825
TseFI GTSAC 1 cut(s) 657
TseI GCWGC 3 cut(s) 191, 317, 320
Tsp45I GTSAC 1 cut(s) 657
TspDTI ATGAA 8 cut(s) 39, 107, 296, 524, 552, 603, 660, 818
TspRI CASTG 3 cut(s) 666, 693, 825
Van91I CCANNNNNTGG 1 cut(s) 137
Vha464I CTTAAG 1 cut(s) 1031
VpaK11BI GGWCC 2 cut(s) 200, 619
XapI RAATTY 2 cut(s) 305, 667
XceI RCATGY 1 cut(s) 990
XspI CTAG 2 cut(s) 152, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.