Rorug02G0333300

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000338
Physical Location & Seq
Reverse (-)
41284499 .. 41289303
4805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0333300.1

Sequence Viewer

Length: 2358 bp
ATGACCCAAGTCGAAGGAGAGGAGGAGCCACAGAACCCTAATTCCCAATTTGAAACACTCACCCTACAAATGAAGCCTCAACCCGACTCAAACCAAGACAACCCCGACGCCTTCCCCGTCCGATCTCCTCGGAGCCGGACCGCCATAACCTTCGCTCGCAAGAAGCGCAAGAAGACGGGCAGGAATCCGAAAGCCGCTCAGAAGAAGCTGGAGATTCTGCGCCAAACCCTAAAGCCAATCCCCTTCGTCCCCTCCAAAACCCTAGACTTCGAGCGCCACGAGGAGCTCCTGAAGCGGCTGGGCTTGTGGGACTTCGTCCACATCAAGTTCGATCGGAGCATTAGGGCTGATCTCCTCGCCCAATTGATCGTCAACTTCAACTCCCAGCAGCGGTGTAGCTACGTCAACGAGCTTAGGATTCTTGTCAACCGGTCCGATCTGGGACGCGCATTGAAGCTTCCGGTGACGCTCAAGTCCAAGAAGAGCGCCGTGGAAGATGCCGGCGAGGAGCAGCCGCCGGCGTCGGAGGAGTCGATAGCTTTTCTGGAGGATTTGGTTTCGAATTGGATGCTTTTGCACGGGGATATGTGGATGATGCCCAGCGAGGTGTTGGGTTATACCAAAGCAATCAAAGAAGGTCAGTTTGGGAAGGTGGACTGGGCTGGTTTGGTTTGGTACATGGTGAATGAGGAGCTAGGTAAGGCCACGCAGTTGGAGAATTGTTACTATGCGTCGCATTTGCAGCATTTGATCAAGTCGCAGAAGGAGGATTTGTTTTCGGTGAAGCAAGAGTCGAAAGTGGAGATTGATTTGAAGGATGATGATGAGGAAGAAGAGGAAGAGGATGGTGGTGGGGATGTGAAAATGGATGATGTTCAGGGAGGCAGATTGGAGGAGAATGATATTAAGTTGTGTCTCGGGCAAGAAAATGTGACCAAAGTTGAGGTTGAGCACGAAAACTTGGAGAAAGTTGATGTCGAGCAAGAGAATGTGGAGAATGTTGATAATGAGCGAGAAGATGCGGAGAAAGTTGATGTTGGGAAAGAGAATGTGGAGAAAGATGATGTCGGGCAAGAGAAGGTGGAGGAAGTTGAGGCTGAGCAAGAAAATGTAGAGAAAGTTGATTCTCAGCAAGGAAATGCGGAGAAAGTTGACGTTGGAGAAGACAATGTAGAGAAAGGTTGTGGTGAAACAGAGCGGGTTGAGGTTGAGGTCCAGGATGAGGATGTGATGGATTTTGAAGAATTTAAGGAGGCAGAGCCTGCCCAATGGGTTTTGGCTGGGAAGGACAATCTAGGTGAGCCTTGTTTGCAGCGGTGTAATCTCGGTGGTATTAATGATCTTGGTTGTGGGGATGAGAGAAATAAAAATATGGAGTTGGGAGAAGGAGTAGGTGAAGAAGAACAAGAAGATGATGAAATAGAAGAAGAGGAAGAGGAGGATGATGATCAGGAGGGTGGTTTCCACCTTTTGCCTAAAGGTTTTTCTATAGAGGGGTTTCCTTCAGGAAATCTTACTCAGGGTATGGATGGTATGCCACTGCGTGATCATTTTGGCATAGAATTTCCTTCTCCAAGGGACCTAATGCTTCCCGGAAGTTCTTCCATTTATGGTAATGGTCCCAAGAGAGAGATTAACCATGAGAATGATAATTCTCATCATGGTCTCAATGGTAATAAGAGGTTGAGGACAGAAGGCTCTTGGGATAGTAAAATGTCTGGTGACTTTGAGACAGGTATGGATCAAATACAGCAGTGGATGGGGAGAGTGGAGGATATGCAGCAGTGGATGGGAAAAGCTAGGATGATGTATGCAGCAAAAGAACAGGAGTGCCAACAAGCTGCAAATAACCAGCAGTATTTTATGCAAGAGTTGCAGAGAAGGGATGAGATGATTGAGCACTTGCATAAGGCCAGGTTTGAAGAGCAGCAAAAGTTTCAAACAGAGAAGTTTAGGTTTGAGCATGAGGTCCACTTGATGAAAAATCTCTTGGACGGCTATAGGAAAGCTTTAAAGGCAACACAAAAGGCATTTGCTGAACATAGAGCACATTGTCCACAGGCTGTTGAGCCACTCTACAGGGATGTTCCGGGATCCGGGGGCCTTGTTTTAAGCACCACAGAACTGGAAAAGCTACGCATAAAGCAAGAAGAAGAAGAGAGGATGAATAGACTGGCGATTGAAACGATGATTGAGGACTTTGAAGCTGGGTGGAGTAGTGAATATGAAGCACACCAAAAGAGTGTTGAATTAGCTAGTAGCAGGTTGATGGATGTTGAGAAGGAAGTGAAACTTCTCAAGGAAGGCCTTTTAAAACAGAGGCTTCCAGAAACACAAGAATCTCCAAATGAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

785

Amino Acids

89.97

Weight (kDa)

4.65

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 472
Acc36I ACCTGC 1 cut(s) 2253
AccBSI CCGCTC 2 cut(s) 197, 1198
AccII CGCG 1 cut(s) 447
AciI CCGC 9 cut(s) 141, 195, 295, 391, 515, 1022, 1142, 1198, 1315
AclWI GGATC 3 cut(s) 1749, 2088, 2101
AcsI RAATTY 2 cut(s) 1244, 1562
AcuI CTGAAG 2 cut(s) 311, 1488
AcyI GRCGYC 2 cut(s) 108, 521
AdeI CACNNNGTG 1 cut(s) 1544
AfaI GTAC 1 cut(s) 677
AfiI CCNNNNNNNGG 3 cut(s) 390, 1222, 2096
AgeI ACCGGT 1 cut(s) 429
AjnI CCWGG 2 cut(s) 1215, 1913
AjuI GAANNNNNNNTTGG 4 cut(s) 627, 659, 1973, 2005
AloI GAACNNNNNNTCC 2 cut(s) 26, 58
Alw21I GWGCWC 4 cut(s) 288, 954, 1902, 2050
Alw26I GTCTC 3 cut(s) 920, 1670, 1724
AlwI GGATC 3 cut(s) 1749, 2088, 2101
AlwNI CAGNNNCTG 1 cut(s) 1262
Ama87I CYCGRG 1 cut(s) 917
AoxI GGCC 4 cut(s) 702, 1911, 2101, 2305
ApeKI GCWGC 8 cut(s) 388, 511, 742, 1312, 1780, 1814, 1841, 1927
ApoI RAATTY 2 cut(s) 1244, 1562
AseI ATTAAT 1 cut(s) 1335
AsiGI ACCGGT 1 cut(s) 429
Asp700I GAANNNNTTC 1 cut(s) 1600
AspLEI GCGC 5 cut(s) 168, 222, 276, 449, 488
AspS9I GGNCC 7 cut(s) 138, 432, 1213, 1579, 1619, 1969, 2101
AsuC2I CCSGG 3 cut(s) 1593, 2091, 2098
AsuHPI GGTGA 8 cut(s) 52, 475, 694, 793, 1199, 1310, 1406, 1733
AsuII TTCGAA 1 cut(s) 560
AvaI CYCGRG 1 cut(s) 917
AvaII GGWCC 6 cut(s) 138, 432, 1213, 1579, 1619, 1969
BamHI GGATCC 1 cut(s) 2093
BanII GRGCYC 1 cut(s) 288
BauI CACGAG 1 cut(s) 278
BbsI GAAGAC 2 cut(s) 179, 1170
Bbv12I GWGCWC 4 cut(s) 288, 954, 1902, 2050
BbvI GCAGC 8 cut(s) 400, 523, 754, 1324, 1792, 1826, 1828, 1939
BccI CCATC 6 cut(s) 839, 1225, 1523, 1753, 1783, 2263
BceAI ACGGC 2 cut(s) 473, 2011
BcgI CGANNNNNNTGC 2 cut(s) 550, 584
BciT130I CCWGG 2 cut(s) 1217, 1915
BclI TGATCA 3 cut(s) 750, 1447, 1546
BcnI CCSGG 3 cut(s) 1593, 2091, 2098
BcoDI GTCTC 3 cut(s) 920, 1670, 1724
BfaI CTAG 5 cut(s) 263, 695, 1295, 1800, 2256
BfmI CTRYAG 3 cut(s) 1488, 1999, 2077
BfoI RGCGCY 2 cut(s) 277, 489
BfuAI ACCTGC 1 cut(s) 2253
BlpI GCTNAGC 1 cut(s) 1098
Bme1390I CCNGG 5 cut(s) 1217, 1593, 1915, 2091, 2098
Bme18I GGWCC 6 cut(s) 138, 432, 1213, 1579, 1619, 1969
BmeT110I CYCGRG 1 cut(s) 917
BmgT120I GGNCC 7 cut(s) 138, 432, 1213, 1579, 1619, 1969, 2101
BmiI GGNNCC 6 cut(s) 27, 134, 1580, 1621, 2095, 2102
BmrFI CCNGG 5 cut(s) 1217, 1593, 1915, 2091, 2098
BmrI ACTGGG 1 cut(s) 667
BmsI GCATC 4 cut(s) 487, 558, 585, 1009
BmuI ACTGGG 1 cut(s) 667
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 2 cut(s) 179, 1170
BpmI CTGGAG 2 cut(s) 230, 566
Bpu10I CCTNAGC 1 cut(s) 413
Bpu1102I GCTNAGC 1 cut(s) 1098
Bpu14I TTCGAA 1 cut(s) 560
BpuEI CTTGAG 2 cut(s) 455, 2282
BpuMI CCSGG 3 cut(s) 1593, 2091, 2098
BsaBI GATNNNNATC 1 cut(s) 1446
BsaHI GRCGYC 2 cut(s) 108, 521
BsaI GGTCTC 1 cut(s) 1670
BsaJI CCNNGG 4 cut(s) 128, 489, 1574, 2097
BsaWI WCCGGW 2 cut(s) 429, 460
BsaXI ACNNNNNCTCC 6 cut(s) 365, 395, 758, 788, 1374, 1404
Bsc4I CCNNNNNNNGG 3 cut(s) 390, 1222, 2096
Bse118I RCCGGY 3 cut(s) 429, 500, 517
Bse1I ACTGG 3 cut(s) 662, 2130, 2178
Bse8I GATNNNNATC 1 cut(s) 1446
BseBI CCWGG 2 cut(s) 1217, 1915
BseDI CCNNGG 4 cut(s) 128, 489, 1574, 2097
BseJI GATNNNNATC 1 cut(s) 1446
BseLI CCNNNNNNNGG 3 cut(s) 390, 1222, 2096
BseMII CTCAG 4 cut(s) 212, 1089, 1142, 1532
BseNI ACTGG 3 cut(s) 662, 2130, 2178
BseXI GCAGC 8 cut(s) 400, 523, 754, 1324, 1792, 1826, 1828, 1939
BseYI CCCAGC 5 cut(s) 298, 384, 599, 1280, 2207
Bsh1236I CGCG 1 cut(s) 447
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 4 cut(s) 704, 1913, 2103, 2307
BshTI ACCGGT 1 cut(s) 429
BsiEI CGRYCG 1 cut(s) 334
BsiHKAI GWGCWC 4 cut(s) 288, 954, 1902, 2050
BsiHKCI CYCGRG 1 cut(s) 917
BsiSI CCGG 8 cut(s) 136, 430, 461, 501, 518, 1593, 2090, 2097
BslFI GGGAC 5 cut(s) 233, 323, 456, 1592, 1605
BslI CCNNNNNNNGG 3 cut(s) 390, 1222, 2096
BsmAI GTCTC 3 cut(s) 920, 1670, 1724
BsmFI GGGAC 5 cut(s) 233, 323, 456, 1592, 1605
BsnI GGCC 4 cut(s) 704, 1913, 2103, 2307
Bso31I GGTCTC 1 cut(s) 1670
BsoBI CYCGRG 1 cut(s) 917
Bsp119I TTCGAA 1 cut(s) 560
Bsp1286I GDGCHC 4 cut(s) 288, 954, 1902, 2050
Bsp1720I GCTNAGC 1 cut(s) 1098
BspACI CCGC 9 cut(s) 141, 195, 295, 391, 515, 1022, 1142, 1198, 1315
BspANI GGCC 4 cut(s) 704, 1913, 2103, 2307
BspCNI CTCAG 4 cut(s) 211, 1090, 1141, 1531
BspFNI CGCG 1 cut(s) 447
BspHI TCATGA 1 cut(s) 2354
BspLI GGNNCC 6 cut(s) 27, 134, 1580, 1621, 2095, 2102
BspMI ACCTGC 1 cut(s) 2253
BspPI GGATC 3 cut(s) 1749, 2088, 2101
BspQI GCTCTTC 2 cut(s) 476, 1917
BspT104I TTCGAA 1 cut(s) 560
BspTNI GGTCTC 1 cut(s) 1670
BsrBI CCGCTC 2 cut(s) 197, 1198
BsrFI RCCGGY 3 cut(s) 429, 500, 517
BsrI ACTGG 3 cut(s) 662, 2130, 2178
BssAI RCCGGY 3 cut(s) 429, 500, 517
BssECI CCNNGG 4 cut(s) 128, 489, 1574, 2097
BssNI GRCGYC 2 cut(s) 108, 521
BssSI CACGAG 1 cut(s) 278
BssT1I CCWWGG 1 cut(s) 1574
Bst2BI CACGAG 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 1217, 1915
Bst6I CTCTTC 7 cut(s) 476, 828, 834, 1422, 1428, 1917, 2151
BstACI GRCGYC 2 cut(s) 108, 521
BstAPI GCANNNNNTGC 2 cut(s) 1262, 1873
BstBI TTCGAA 1 cut(s) 560
BstC8I GCNNGC 4 cut(s) 157, 502, 519, 1263
BstDEI CTNAG 5 cut(s) 198, 413, 1098, 1128, 1518
BstDSI CCRYGG 1 cut(s) 489
BstFNI CGCG 1 cut(s) 447
BstH2I RGCGCY 2 cut(s) 277, 489
BstHHI GCGC 5 cut(s) 168, 222, 276, 449, 488
BstMAI GTCTC 3 cut(s) 920, 1670, 1724
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 7 cut(s) 165, 292, 742, 1262, 1309, 1873, 2015
BstNI CCWGG 2 cut(s) 1217, 1915
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 5 cut(s) 1215, 1591, 1913, 2089, 2096
BstSFI CTRYAG 3 cut(s) 1488, 1999, 2077
BstUI CGCG 1 cut(s) 447
BstV1I GCAGC 8 cut(s) 400, 523, 754, 1324, 1792, 1826, 1828, 1939
BstV2I GAAGAC 2 cut(s) 179, 1170
BstX2I RGATCY 1 cut(s) 2093
BstXI CCANNNNNNTGG 2 cut(s) 712, 2125
BstYI RGATCY 1 cut(s) 2093
BsuRI GGCC 4 cut(s) 704, 1913, 2103, 2307
BtgI CCRYGG 1 cut(s) 489
BtsI GCAGTG 3 cut(s) 1538, 1760, 1790
BtsIMutI CAGTG 3 cut(s) 1538, 1760, 1790
BveI ACCTGC 1 cut(s) 2253
Cac8I GCNNGC 4 cut(s) 157, 502, 519, 1263
CaiI CAGNNNCTG 1 cut(s) 1262
CciI TCATGA 1 cut(s) 2354
CfoI GCGC 5 cut(s) 168, 222, 276, 449, 488
Cfr10I RCCGGY 3 cut(s) 429, 500, 517
Cfr13I GGNCC 7 cut(s) 138, 432, 1213, 1579, 1619, 1969, 2101
CpoI CGGWCCG 2 cut(s) 138, 432
CseI GACGC 5 cut(s) 116, 453, 475, 510, 720
Csp6I GTAC 1 cut(s) 676
CspAI ACCGGT 1 cut(s) 429
CspI CGGWCCG 2 cut(s) 138, 432
CviAII CATG 5 cut(s) 679, 1640, 1661, 1964, 2355
CviQI GTAC 1 cut(s) 676
DdeI CTNAG 5 cut(s) 198, 413, 1098, 1128, 1518
DraI TTTAAA 2 cut(s) 2013, 2313
DraIII CACNNNGTG 1 cut(s) 1544
DrdI GACNNNNNNGTC 1 cut(s) 472
DseDI GACNNNNNNGTC 1 cut(s) 472
Eam1104I CTCTTC 7 cut(s) 476, 828, 834, 1422, 1428, 1917, 2151
EarI CTCTTC 7 cut(s) 476, 828, 834, 1422, 1428, 1917, 2151
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 1574
Eco147I AGGCCT 1 cut(s) 2307
Eco24I GRGCYC 1 cut(s) 288
Eco31I GGTCTC 1 cut(s) 1670
Eco47I GGWCC 6 cut(s) 138, 432, 1213, 1579, 1619, 1969
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 2 cut(s) 311, 1488
Eco88I CYCGRG 1 cut(s) 917
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 2 cut(s) 1579, 2101
EcoRII CCWGG 2 cut(s) 1215, 1913
EcoT14I CCWWGG 1 cut(s) 1574
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 1574
FaeI CATG 5 cut(s) 682, 1643, 1664, 1967, 2358
FalI AAGNNNNNCTT 2 cut(s) 2277, 2309
FaqI GGGAC 5 cut(s) 233, 323, 456, 1592, 1605
FatI CATG 5 cut(s) 678, 1639, 1660, 1963, 2354
FauI CCCGC 1 cut(s) 1191
FbaI TGATCA 3 cut(s) 750, 1447, 1546
FriOI GRGCYC 1 cut(s) 288
FspBI CTAG 5 cut(s) 263, 695, 1295, 1800, 2256
GlaI GCGC 5 cut(s) 167, 221, 275, 448, 487
GsaI CCCAGC 5 cut(s) 302, 388, 603, 1284, 2211
GsuI CTGGAG 2 cut(s) 230, 566
HaeII RGCGCY 2 cut(s) 277, 489
HaeIII GGCC 4 cut(s) 704, 1913, 2103, 2307
HapII CCGG 8 cut(s) 136, 430, 461, 501, 518, 1593, 2090, 2097
HgaI GACGC 5 cut(s) 116, 453, 475, 510, 720
HhaI GCGC 5 cut(s) 168, 222, 276, 449, 488
Hin1I GRCGYC 2 cut(s) 108, 521
Hin1II CATG 5 cut(s) 682, 1643, 1664, 1967, 2358
Hin6I GCGC 5 cut(s) 166, 220, 274, 447, 486
HinP1I GCGC 5 cut(s) 166, 220, 274, 447, 486
HincII GTYRAC 4 cut(s) 373, 406, 427, 1153
HindII GTYRAC 4 cut(s) 373, 406, 427, 1153
HindIII AAGCTT 2 cut(s) 455, 2007
HinfI GANTC 9 cut(s) 86, 184, 214, 418, 530, 791, 1124, 2339, 2351
HpaII CCGG 8 cut(s) 136, 430, 461, 501, 518, 1593, 2090, 2097
HphI GGTGA 8 cut(s) 52, 475, 694, 793, 1199, 1310, 1406, 1733
Hpy166II GTNNAC 8 cut(s) 319, 373, 406, 427, 655, 1153, 1972, 2057
Hpy188I TCNGA 7 cut(s) 122, 132, 189, 201, 336, 436, 526
Hpy188III TCNNGA 6 cut(s) 289, 545, 1451, 1506, 2327, 2355
Hpy8I GTNNAC 8 cut(s) 319, 373, 406, 427, 655, 1153, 1972, 2057
Hpy99I CGWCG 3 cut(s) 110, 526, 736
HpyCH4IV ACGT 2 cut(s) 402, 1155
HpyCH4V TGCA 9 cut(s) 577, 742, 1312, 1780, 1814, 1844, 1867, 1876, 1906
HpyF10VI GCNNNNNNNGC 7 cut(s) 165, 292, 742, 1262, 1309, 1873, 2015
HpyF3I CTNAG 5 cut(s) 198, 413, 1098, 1128, 1518
HpySE526I ACGT 2 cut(s) 402, 1155
Hsp92I GRCGYC 2 cut(s) 108, 521
Hsp92II CATG 5 cut(s) 682, 1643, 1664, 1967, 2358
HspAI GCGC 5 cut(s) 166, 220, 274, 447, 486
KroI GCCGGC 2 cut(s) 500, 517
KroNI GCCGGC 2 cut(s) 502, 519
Ksp22I TGATCA 3 cut(s) 750, 1447, 1546
LguI GCTCTTC 2 cut(s) 476, 1917
LmnI GCTCC 7 cut(s) 25, 132, 283, 291, 336, 508, 691
Lsp1109I GCAGC 8 cut(s) 400, 523, 754, 1324, 1792, 1826, 1828, 1939
LweI GCATC 4 cut(s) 487, 558, 585, 1009
MaeI CTAG 5 cut(s) 263, 695, 1295, 1800, 2256
MaeII ACGT 2 cut(s) 402, 1155
MaeIII GTNAC 4 cut(s) 463, 722, 931, 1721
MbiI CCGCTC 2 cut(s) 197, 1198
MfeI CAATTG 1 cut(s) 362
MflI RGATCY 1 cut(s) 2093
MhlI GDGCHC 4 cut(s) 288, 954, 1902, 2050
MluCI AATT 9 cut(s) 40, 47, 362, 562, 718, 1244, 1562, 1651, 2249
MlyI GAGTC 3 cut(s) 80, 539, 800
MmeI TCCRAC 3 cut(s) 504, 693, 1138
MreI CGCCGGCG 1 cut(s) 517
MroNI GCCGGC 2 cut(s) 500, 517
MroXI GAANNNNTTC 1 cut(s) 1600
MseI TTAA 7 cut(s) 906, 1248, 1335, 1635, 2012, 2111, 2312
MslI CAYNNNNRTG 1 cut(s) 1644
MspA1I CMGCKG 2 cut(s) 391, 1315
MspI CCGG 8 cut(s) 136, 430, 461, 501, 518, 1593, 2090, 2097
MspR9I CCNGG 5 cut(s) 1217, 1593, 1915, 2091, 2098
MunI CAATTG 1 cut(s) 362
MvaI CCWGG 2 cut(s) 1217, 1915
MvnI CGCG 1 cut(s) 447
MwoI GCNNNNNNNGC 7 cut(s) 165, 292, 742, 1262, 1309, 1873, 2015
NaeI GCCGGC 2 cut(s) 502, 519
NciI CCSGG 3 cut(s) 1593, 2091, 2098
NgoMIV GCCGGC 2 cut(s) 500, 517
NlaIII CATG 5 cut(s) 682, 1643, 1664, 1967, 2358
NlaIV GGNNCC 6 cut(s) 27, 134, 1580, 1621, 2095, 2102
NmuCI GTSAC 3 cut(s) 463, 931, 1721
NspV TTCGAA 1 cut(s) 560
PagI TCATGA 1 cut(s) 2354
PceI AGGCCT 1 cut(s) 2307
PciSI GCTCTTC 2 cut(s) 476, 1917
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 276, 530
PdiI GCCGGC 2 cut(s) 502, 519
PdmI GAANNNNTTC 1 cut(s) 1600
PfeI GAWTC 6 cut(s) 184, 214, 418, 1124, 2339, 2351
PflFI GACNNNGTC 1 cut(s) 314
PfoI TCCNGGA 3 cut(s) 1215, 1591, 2089
PinAI ACCGGT 1 cut(s) 429
Ple19I CGATCG 1 cut(s) 334
PleI GAGTC 3 cut(s) 80, 538, 799
PpsI GAGTC 3 cut(s) 80, 538, 799
PpuMI RGGWCCY 1 cut(s) 1579
PshAI GACNNNNGTC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 1335
Psp124BI GAGCTC 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 1579
Psp6I CCWGG 2 cut(s) 1215, 1913
PspFI CCCAGC 5 cut(s) 298, 384, 599, 1280, 2207
PspGI CCWGG 2 cut(s) 1215, 1913
PspN4I GGNNCC 6 cut(s) 27, 134, 1580, 1621, 2095, 2102
PspPI GGNCC 7 cut(s) 138, 432, 1213, 1579, 1619, 1969, 2101
PspPPI RGGWCCY 1 cut(s) 1579
PstNI CAGNNNCTG 1 cut(s) 1262
PsuI RGATCY 1 cut(s) 2093
PsyI GACNNNGTC 1 cut(s) 314
PvuI CGATCG 1 cut(s) 334
RsaI GTAC 1 cut(s) 677
RsaNI GTAC 1 cut(s) 676
RseI CAYNNNNRTG 1 cut(s) 1644
Rsr2I CGGWCCG 2 cut(s) 138, 432
RsrII CGGWCCG 2 cut(s) 138, 432
SacI GAGCTC 1 cut(s) 288
SapI GCTCTTC 2 cut(s) 476, 1917
SaqAI TTAA 7 cut(s) 906, 1248, 1335, 1635, 2012, 2111, 2312
Sau96I GGNCC 7 cut(s) 138, 432, 1213, 1579, 1619, 1969, 2101
SchI GAGTC 3 cut(s) 80, 539, 800
ScrFI CCNGG 5 cut(s) 1217, 1593, 1915, 2091, 2098
SduI GDGCHC 4 cut(s) 288, 954, 1902, 2050
SfaNI GCATC 4 cut(s) 487, 558, 585, 1009
SfcI CTRYAG 3 cut(s) 1488, 1999, 2077
SfuI TTCGAA 1 cut(s) 560
SgrAI CRCCGGYG 1 cut(s) 517
SinI GGWCC 6 cut(s) 138, 432, 1213, 1579, 1619, 1969
SmiMI CAYNNNNRTG 1 cut(s) 1644
SmlI CTYRAG 2 cut(s) 470, 2297
SmoI CTYRAG 2 cut(s) 470, 2297
Sse9I AATT 9 cut(s) 40, 47, 362, 562, 718, 1244, 1562, 1651, 2249
SseBI AGGCCT 1 cut(s) 2307
SsiI CCGC 9 cut(s) 141, 195, 295, 391, 515, 1022, 1142, 1198, 1315
SspMI CTAG 5 cut(s) 263, 695, 1295, 1800, 2256
SstI GAGCTC 1 cut(s) 288
StuI AGGCCT 1 cut(s) 2307
StyD4I CCNGG 5 cut(s) 1215, 1591, 1913, 2089, 2096
StyI CCWWGG 1 cut(s) 1574
TaiI ACGT 2 cut(s) 405, 1158
TaqI TCGA 7 cut(s) 12, 270, 330, 533, 560, 794, 978
TasI AATT 9 cut(s) 40, 47, 362, 562, 718, 1244, 1562, 1651, 2249
TauI GCSGC 3 cut(s) 197, 298, 517
TfiI GAWTC 6 cut(s) 184, 214, 418, 1124, 2339, 2351
Tru1I TTAA 7 cut(s) 906, 1248, 1335, 1635, 2012, 2111, 2312
Tru9I TTAA 7 cut(s) 906, 1248, 1335, 1635, 2012, 2111, 2312
TscAI CASTG 3 cut(s) 1545, 1760, 1790
TseFI GTSAC 3 cut(s) 463, 931, 1721
TseI GCWGC 8 cut(s) 388, 511, 742, 1312, 1780, 1814, 1841, 1927
Tsp45I GTSAC 3 cut(s) 463, 931, 1721
TspDTI ATGAA 5 cut(s) 86, 1431, 1994, 2180, 2241
TspRI CASTG 3 cut(s) 1545, 1760, 1790
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 6 cut(s) 138, 432, 1213, 1579, 1619, 1969
VspI ATTAAT 1 cut(s) 1335
XapI RAATTY 2 cut(s) 1244, 1562
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmnI GAANNNNTTC 1 cut(s) 1600
XspI CTAG 5 cut(s) 263, 695, 1295, 1800, 2256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.