FvH4_6g30450

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000338
Physical Location & Seq
Forward (+)
23569872 .. 23574930
5059 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30450.t1

Sequence Viewer

Length: 1971 bp
ATGACGACACGGCATGTCGTTAAGTTTGTTAAACGCACCAGGTGGCAGGCGTTTCTTCCATTTCCATTACCCACTTCATTCCTTCACAAACAAGTCTTTCTCTTCTCCTCCTCCTCCTCCTCCTCCTTCAATCTTCACCATCACAGAGCTCCTCTGCATTTTCACCTCAGGTTGAAACTTGGACCAAGTGGTACATTAGAGTTCACAGAGATATGTTTTGTGCAGGATGGGAGTAGTTCTGGTGTGGAAGACAATGATATTGATTGGAACACAGAAGATGAGCTTGAATTGGAGAATTTCACGTTATCTCATTCTTCAAGTTTGGTACTTACGGGTGGCGAAGCTACTGCCGTCTCTGGGGAGATGGGTTCATGTCCAAGTTCTTCCAATTCCAAGGTTCTTGATCAGTTTATAGGGATGGAATTTTCTAAGAAGATGGTTGAAAAAGCAATTGAGGAACATGGAGAAGAAAATACAGAGGCAATTCTGGAAACACTCCTCACCTATTCGGTTCTAGAAAGTTCTTCCCAAGAACAGCAACAAACCGATTCAGATCAGTTTTCCTCTGATTACGAAGGAAGCTTTCCGGATGACTTTTCAGATTTGGACAGCTCTAGTGATGAGGAAATCATGAGTTCTGCATCTGAGGAGGTAAAGCTACTCATGTCCTTAATCAAAATGGGGTACACAGAAGAGGAGGCTTCCATAGCTATAGAAAGATGTGGTATGAGCTCGTCACTTCTGGAATTGACTGATTTTTTATCTGCTTCTCAATTGGCAAAGGCAGAAGATGCCCTTCTTCCCCTTGAAGAAAAGCCTGGACATCTGCACACTGACTATGCTAAGAAGAAAAGGAAACTTCTGGTTGATGAAGTGTCGAAAAGGAAGAGGCAGAACAGGCTCATAGGGGAAGATAATGAGACAGTTCGTCTCCCAAAGCCAATGATTGGGTTCGGTATCCCAGCTGGACCATGCCAATCTTACAGAAATCTTCCAAAGCCAGCCATAGGACCTCCCTATTTTTATTATGAGAATGTAGCCCTTACTCCTAAAGGGGTTTGGGACACTATATCCAGATTCCTATATGACGTTCCACCTGAATTTGTTGACTCTAAGTACTTCTGTGCTGCTGCCAGAAAAAGAGGTTATGTTCACAATCTTCCAGTAGAGAATAGATTTCCTCTTGTTCCCCTTCCCCCGCAAACCATACATGAGGCATTGCCCTTGTCAAGAAAGTGGTGGCCTTCATGGGACACTCGGACAAAGCTAAATTGCTTAATAACATGCCATTCAAGTGCAAAATTAACAGAGAGGATCATGAAGGCTCTTGAGGGCTACGATGACATTCCACCGGAACGAGTCCAGCGAGGTGTATTGAATGAATGCCGTAAGTGGAATCTAGTGTGGGTTGGAAAAAATAGGGTTGCCCCTCTTGAGCCTGATGAATTTGAAATGCTTTTGGGGTTCCCAAAGAACCACACCAGGGGAATAAGCAGGACTGACAGATATAAATCACTCGGGAATGCATTTCAGATTGACACTGTGGCATACCACTTCTCTGTTTTGCGAGACATGTTTCCTAATGGCATGAATGTCCTCTCTCTTTTCTCTGGGATAGGTGGGGCAGAGATAGCCCTTCATCGGCTTGGTATCCGTATGAAGAATGTTGTTTCTCTAGAGATTTCAGAAGTGAACAGAAACGTCGTGAGGTGTTGGTGGGAGCAAACAAATCAAACCGGGAATTTGATTCACCTTCCTGATGTGCAAGAGCTGAATGCTGACCGCTTGGAACAGTTTATGAGTGCATTTGGTGGATTCGATCTAGTAGTTGGTGGCAGTCCCTGTAACAATCTAACAGGCAGCAACAGATATCATCGAGATGGACTCGAGGGTAAAGAATCGTCACTTTTCTATCATTACTTTCGCATACTGGACTTGGTTAAGTCTATGATGGCAGAGGGATATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

657

Amino Acids

74.23

Weight (kDa)

5.58

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 530 - 645 2.7e-08 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 947
AccIII TCCGGA 1 cut(s) 586
AciI CCGC 2 cut(s) 1199, 1783
AclWI GGATC 1 cut(s) 1322
AcsI RAATTY 5 cut(s) 295, 422, 1100, 1445, 1741
AdeI CACNNNGTG 1 cut(s) 42
AfaI GTAC 4 cut(s) 193, 327, 686, 1118
AfiI CCNNNNNNNGG 5 cut(s) 357, 947, 1007, 1483, 1641
AflIII ACRYGT 1 cut(s) 1572
AgsI TTSAA 9 cut(s) 130, 175, 287, 318, 443, 809, 1293, 1378, 1451
AhdI GACNNNNNGTC 1 cut(s) 927
AjnI CCWGG 3 cut(s) 38, 817, 1481
Alw21I GWGCWC 2 cut(s) 151, 734
Alw26I GTCTC 4 cut(s) 358, 914, 935, 1563
AlwI GGATC 1 cut(s) 1322
AlwNI CAGNNNCTG 1 cut(s) 1842
Ama87I CYCGRG 2 cut(s) 1517, 1886
Aor13HI TCCGGA 1 cut(s) 586
AoxI GGCC 1 cut(s) 1241
ApeKI GCWGC 3 cut(s) 1127, 1130, 1860
ApoI RAATTY 5 cut(s) 295, 422, 1100, 1445, 1741
AspS9I GGNCC 3 cut(s) 182, 968, 1010
AsuC2I CCSGG 1 cut(s) 1738
AsuHPI GGTGA 4 cut(s) 128, 155, 493, 1742
AvaI CYCGRG 2 cut(s) 1517, 1886
AvaII GGWCC 3 cut(s) 182, 968, 1010
AxyI CCTNAGG 1 cut(s) 167
BanII GRGCYC 2 cut(s) 151, 734
BbsI GAAGAC 1 cut(s) 255
Bbv12I GWGCWC 2 cut(s) 151, 734
BbvI GCAGC 3 cut(s) 1114, 1117, 1872
BccI CCATC 7 cut(s) 147, 221, 358, 412, 430, 1874, 1945
BceAI ACGGC 3 cut(s) 26, 335, 1371
BcgI CGANNNNNNTGC 2 cut(s) 329, 363
BciT130I CCWGG 3 cut(s) 40, 819, 1483
BciVI GTATCC 2 cut(s) 968, 1661
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 1 cut(s) 1738
BcoDI GTCTC 4 cut(s) 358, 914, 935, 1563
BfaI CTAG 5 cut(s) 515, 615, 1400, 1676, 1823
BfmI CTRYAG 1 cut(s) 711
BfuI GTATCC 2 cut(s) 968, 1661
BisI GCNGC 3 cut(s) 1128, 1131, 1861
BlsI GCNGC 3 cut(s) 1129, 1132, 1862
BmcAI AGTACT 1 cut(s) 1118
Bme1390I CCNGG 4 cut(s) 40, 819, 1483, 1738
Bme18I GGWCC 3 cut(s) 182, 968, 1010
BmeRI GACNNNNNGTC 1 cut(s) 927
BmeT110I CYCGRG 2 cut(s) 1517, 1886
BmgT120I GGNCC 3 cut(s) 182, 968, 1010
BmiI GGNNCC 1 cut(s) 1466
BmrFI CCNGG 4 cut(s) 40, 819, 1483, 1738
BmsI GCATC 2 cut(s) 650, 781
BpiI GAAGAC 1 cut(s) 255
BplI GAGNNNNNCTC 2 cut(s) 1869, 1901
BpuEI CTTGAG 2 cut(s) 1349, 1454
BpuMI CCSGG 1 cut(s) 1738
BsaBI GATNNNNATC 2 cut(s) 552, 1510
BsaJI CCNNGG 2 cut(s) 393, 1482
BsaWI WCCGGW 2 cut(s) 586, 1351
Bsc4I CCNNNNNNNGG 5 cut(s) 357, 947, 1007, 1483, 1641
Bse1I ACTGG 2 cut(s) 1163, 1935
Bse21I CCTNAGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 1217
Bse8I GATNNNNATC 2 cut(s) 552, 1510
BseAI TCCGGA 1 cut(s) 586
BseBI CCWGG 3 cut(s) 40, 819, 1483
BseDI CCNNGG 2 cut(s) 393, 1482
BseGI GGATG 3 cut(s) 232, 423, 595
BseJI GATNNNNATC 2 cut(s) 552, 1510
BseLI CCNNNNNNNGG 5 cut(s) 357, 947, 1007, 1483, 1641
BseMI GCAATG 1 cut(s) 1217
BseMII CTCAG 2 cut(s) 181, 636
BseNI ACTGG 2 cut(s) 1163, 1935
BseXI GCAGC 3 cut(s) 1114, 1117, 1872
BseYI CCCAGC 1 cut(s) 961
BsgI GTGCAG 2 cut(s) 242, 812
BshFI GGCC 1 cut(s) 1243
BsiHKAI GWGCWC 2 cut(s) 151, 734
BsiHKCI CYCGRG 2 cut(s) 1517, 1886
BsiSI CCGG 3 cut(s) 587, 1352, 1737
BslFI GGGAC 3 cut(s) 1076, 1265, 1824
BslI CCNNNNNNNGG 5 cut(s) 357, 947, 1007, 1483, 1641
BsmAI GTCTC 4 cut(s) 358, 914, 935, 1563
BsmBI CGTCTC 2 cut(s) 358, 935
BsmFI GGGAC 3 cut(s) 1076, 1265, 1824
BsmI GAATGC 3 cut(s) 1388, 1528, 1780
BsnI GGCC 1 cut(s) 1243
BsoBI CYCGRG 2 cut(s) 1517, 1886
Bsp1286I GDGCHC 2 cut(s) 151, 734
Bsp13I TCCGGA 1 cut(s) 586
Bsp143I GATC 4 cut(s) 403, 553, 1314, 1819
BspACI CCGC 2 cut(s) 1199, 1783
BspANI GGCC 1 cut(s) 1243
BspCNI CTCAG 2 cut(s) 180, 637
BspEI TCCGGA 1 cut(s) 586
BspHI TCATGA 2 cut(s) 630, 1317
BspLI GGNNCC 1 cut(s) 1466
BspPI GGATC 1 cut(s) 1322
BsrDI GCAATG 1 cut(s) 1217
BsrI ACTGG 2 cut(s) 1163, 1935
BssECI CCNNGG 2 cut(s) 393, 1482
BssMI GATC 4 cut(s) 403, 553, 1314, 1819
BssT1I CCWWGG 1 cut(s) 393
Bst2UI CCWGG 3 cut(s) 40, 819, 1483
Bst4CI ACNGT 3 cut(s) 925, 1543, 1794
Bst6I CTCTTC 3 cut(s) 107, 687, 881
BstAPI GCANNNNNTGC 1 cut(s) 791
BstC8I GCNNGC 2 cut(s) 48, 1002
BstDEI CTNAG 5 cut(s) 167, 429, 645, 843, 1113
BstF5I GGATG 3 cut(s) 232, 423, 595
BstKTI GATC 4 cut(s) 406, 556, 1317, 1822
BstMAI GTCTC 4 cut(s) 358, 914, 935, 1563
BstMBI GATC 4 cut(s) 403, 553, 1314, 1819
BstMWI GCNNNNNNNGC 4 cut(s) 707, 791, 898, 1631
BstNI CCWGG 3 cut(s) 40, 819, 1483
BstNSI RCATGY 3 cut(s) 17, 1287, 1576
BstSCI CCNGG 4 cut(s) 38, 817, 1481, 1736
BstSFI CTRYAG 1 cut(s) 711
BstV1I GCAGC 3 cut(s) 1114, 1117, 1872
BstV2I GAAGAC 1 cut(s) 255
Bsu36I CCTNAGG 1 cut(s) 167
BsuI GTATCC 2 cut(s) 968, 1661
BsuRI GGCC 1 cut(s) 1243
BtsCI GGATG 3 cut(s) 232, 423, 595
BtsIMutI CAGTG 2 cut(s) 831, 1539
Cac8I GCNNGC 2 cut(s) 48, 1002
CaiI CAGNNNCTG 1 cut(s) 1842
CciI TCATGA 2 cut(s) 630, 1317
Cfr13I GGNCC 3 cut(s) 182, 968, 1010
CsiI ACCWGGT 1 cut(s) 38
Csp6I GTAC 4 cut(s) 192, 326, 685, 1117
CviQI GTAC 4 cut(s) 192, 326, 685, 1117
DdeI CTNAG 5 cut(s) 167, 429, 645, 843, 1113
DpnI GATC 4 cut(s) 405, 555, 1316, 1821
DpnII GATC 4 cut(s) 403, 553, 1314, 1819
DraIII CACNNNGTG 1 cut(s) 42
DriI GACNNNNNGTC 1 cut(s) 927
Eam1104I CTCTTC 3 cut(s) 107, 687, 881
Eam1105I GACNNNNNGTC 1 cut(s) 927
EarI CTCTTC 3 cut(s) 107, 687, 881
Ecl136II GAGCTC 2 cut(s) 149, 732
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 2 cut(s) 151, 734
Eco32I GATATC 1 cut(s) 1871
Eco47I GGWCC 3 cut(s) 182, 968, 1010
Eco53kI GAGCTC 2 cut(s) 149, 732
Eco81I CCTNAGG 1 cut(s) 167
Eco88I CYCGRG 2 cut(s) 1517, 1886
EcoICRI GAGCTC 2 cut(s) 149, 732
EcoO109I RGGNCCY 1 cut(s) 1010
EcoRII CCWGG 3 cut(s) 38, 817, 1481
EcoRV GATATC 1 cut(s) 1871
EcoT14I CCWWGG 1 cut(s) 393
EcoT22I ATGCAT 1 cut(s) 1528
EcoT38I GRGCYC 2 cut(s) 151, 734
ErhI CCWWGG 1 cut(s) 393
Esp3I CGTCTC 2 cut(s) 358, 935
FalI AAGNNNNNCTT 4 cut(s) 267, 299, 780, 812
FaqI GGGAC 3 cut(s) 1076, 1265, 1824
FauI CCCGC 1 cut(s) 1206
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 3 cut(s) 1128, 1131, 1861
FokI GGATG 3 cut(s) 239, 430, 602
FriOI GRGCYC 2 cut(s) 151, 734
Fsp4HI GCNGC 3 cut(s) 1128, 1131, 1861
FspBI CTAG 5 cut(s) 515, 615, 1400, 1676, 1823
GluI GCNGC 3 cut(s) 1128, 1131, 1861
GsaI CCCAGC 1 cut(s) 965
HaeIII GGCC 1 cut(s) 1243
HapII CCGG 3 cut(s) 587, 1352, 1737
HincII GTYRAC 1 cut(s) 1108
HindII GTYRAC 1 cut(s) 1108
HindIII AAGCTT 1 cut(s) 580
HinfI GANTC 9 cut(s) 548, 1077, 1109, 1359, 1396, 1747, 1815, 1884, 1898
HpaII CCGG 3 cut(s) 587, 1352, 1737
HphI GGTGA 4 cut(s) 128, 155, 493, 1742
Hpy166II GTNNAC 5 cut(s) 204, 687, 1108, 1153, 1693
Hpy188I TCNGA 7 cut(s) 553, 568, 601, 646, 1260, 1533, 1687
Hpy8I GTNNAC 5 cut(s) 204, 687, 1108, 1153, 1693
Hpy99I CGWCG 1 cut(s) 1706
HpyAV CCTTC 9 cut(s) 92, 136, 569, 806, 1202, 1254, 1315, 1646, 1763
HpyCH4III ACNGT 3 cut(s) 925, 1543, 1794
HpyCH4IV ACGT 3 cut(s) 302, 1089, 1701
HpyCH4V TGCA 8 cut(s) 157, 223, 641, 829, 1298, 1526, 1765, 1805
HpyF10VI GCNNNNNNNGC 4 cut(s) 707, 791, 898, 1631
HpyF3I CTNAG 5 cut(s) 167, 429, 645, 843, 1113
HpySE526I ACGT 3 cut(s) 302, 1089, 1701
Kpn2I TCCGGA 1 cut(s) 586
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 4 cut(s) 403, 553, 1314, 1819
LmnI GCTCC 2 cut(s) 154, 1720
Lsp1109I GCAGC 3 cut(s) 1114, 1117, 1872
LweI GCATC 2 cut(s) 650, 781
MabI ACCWGGT 1 cut(s) 38
MaeI CTAG 5 cut(s) 515, 615, 1400, 1676, 1823
MaeII ACGT 3 cut(s) 302, 1089, 1701
MaeIII GTNAC 3 cut(s) 735, 1844, 1902
MalI GATC 4 cut(s) 405, 555, 1316, 1821
MboI GATC 4 cut(s) 403, 553, 1314, 1819
MfeI CAATTG 2 cut(s) 450, 773
MhlI GDGCHC 2 cut(s) 151, 734
MlyI GAGTC 3 cut(s) 1103, 1368, 1878
MmeI TCCRAC 1 cut(s) 1390
Mph1103I ATGCAT 1 cut(s) 1528
MroI TCCGGA 1 cut(s) 586
MseI TTAA 6 cut(s) 21, 30, 671, 1277, 1304, 1941
MslI CAYNNNNRTG 2 cut(s) 1293, 1878
MspA1I CMGCKG 1 cut(s) 965
MspI CCGG 3 cut(s) 587, 1352, 1737
MspR9I CCNGG 4 cut(s) 40, 819, 1483, 1738
MunI CAATTG 2 cut(s) 450, 773
Mva1269I GAATGC 3 cut(s) 1388, 1528, 1780
MvaI CCWGG 3 cut(s) 40, 819, 1483
MwoI GCNNNNNNNGC 4 cut(s) 707, 791, 898, 1631
NciI CCSGG 1 cut(s) 1738
NdeII GATC 4 cut(s) 403, 553, 1314, 1819
NlaIV GGNNCC 1 cut(s) 1466
NmuCI GTSAC 2 cut(s) 735, 1902
NsiI ATGCAT 1 cut(s) 1528
NspI RCATGY 3 cut(s) 17, 1287, 1576
PaeR7I CTCGAG 1 cut(s) 1886
PagI TCATGA 2 cut(s) 630, 1317
PciI ACATGT 1 cut(s) 1572
PcsI WCGNNNNNNNCGW 1 cut(s) 1363
PctI GAATGC 3 cut(s) 1388, 1528, 1780
PfeI GAWTC 6 cut(s) 548, 1077, 1396, 1747, 1815, 1898
PflMI CCANNNNNTGG 1 cut(s) 947
PkrI GCNGC 3 cut(s) 1129, 1132, 1862
PleI GAGTC 3 cut(s) 1103, 1367, 1878
PpsI GAGTC 3 cut(s) 1103, 1367, 1878
PpuMI RGGWCCY 1 cut(s) 1010
PscI ACATGT 1 cut(s) 1572
Psp124BI GAGCTC 2 cut(s) 151, 734
Psp5II RGGWCCY 1 cut(s) 1010
Psp6I CCWGG 3 cut(s) 38, 817, 1481
PspFI CCCAGC 1 cut(s) 961
PspGI CCWGG 3 cut(s) 38, 817, 1481
PspN4I GGNNCC 1 cut(s) 1466
PspPI GGNCC 3 cut(s) 182, 968, 1010
PspPPI RGGWCCY 1 cut(s) 1010
PspXI VCTCGAGB 1 cut(s) 1886
PstNI CAGNNNCTG 1 cut(s) 1842
PvuII CAGCTG 1 cut(s) 965
RsaI GTAC 4 cut(s) 193, 327, 686, 1118
RsaNI GTAC 4 cut(s) 192, 326, 685, 1117
RseI CAYNNNNRTG 2 cut(s) 1293, 1878
SacI GAGCTC 2 cut(s) 151, 734
SaqAI TTAA 6 cut(s) 21, 30, 671, 1277, 1304, 1941
SatI GCNGC 3 cut(s) 1128, 1131, 1861
Sau3AI GATC 4 cut(s) 403, 553, 1314, 1819
Sau96I GGNCC 3 cut(s) 182, 968, 1010
ScaI AGTACT 1 cut(s) 1118
SchI GAGTC 3 cut(s) 1103, 1368, 1878
ScrFI CCNGG 4 cut(s) 40, 819, 1483, 1738
SduI GDGCHC 2 cut(s) 151, 734
SexAI ACCWGGT 1 cut(s) 38
SfaNI GCATC 2 cut(s) 650, 781
SfcI CTRYAG 1 cut(s) 711
Sfr274I CTCGAG 1 cut(s) 1886
SinI GGWCC 3 cut(s) 182, 968, 1010
SlaI CTCGAG 1 cut(s) 1886
SmiMI CAYNNNNRTG 2 cut(s) 1293, 1878
SmlI CTYRAG 3 cut(s) 1328, 1433, 1886
SmoI CTYRAG 3 cut(s) 1328, 1433, 1886
SsiI CCGC 2 cut(s) 1199, 1783
SspMI CTAG 5 cut(s) 515, 615, 1400, 1676, 1823
SstI GAGCTC 2 cut(s) 151, 734
StyD4I CCNGG 4 cut(s) 38, 817, 1481, 1736
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 3 cut(s) 925, 1543, 1794
TaiI ACGT 3 cut(s) 305, 1092, 1704
TaqI TCGA 4 cut(s) 878, 1818, 1876, 1887
TatI WGTACW 1 cut(s) 1116
TfiI GAWTC 6 cut(s) 548, 1077, 1396, 1747, 1815, 1898
Tru1I TTAA 6 cut(s) 21, 30, 671, 1277, 1304, 1941
Tru9I TTAA 6 cut(s) 21, 30, 671, 1277, 1304, 1941
TscAI CASTG 2 cut(s) 838, 1546
TseFI GTSAC 2 cut(s) 735, 1902
TseI GCWGC 3 cut(s) 1127, 1130, 1860
Tsp45I GTSAC 2 cut(s) 735, 1902
TspGWI ACGGA 1 cut(s) 1643
TspRI CASTG 2 cut(s) 838, 1546
Van91I CCANNNNNTGG 1 cut(s) 947
VpaK11BI GGWCC 3 cut(s) 182, 968, 1010
XapI RAATTY 5 cut(s) 295, 422, 1100, 1445, 1741
XbaI TCTAGA 2 cut(s) 514, 1675
XceI RCATGY 3 cut(s) 17, 1287, 1576
XhoI CTCGAG 1 cut(s) 1886
XspI CTAG 5 cut(s) 515, 615, 1400, 1676, 1823
ZrmI AGTACT 1 cut(s) 1118
Zsp2I ATGCAT 1 cut(s) 1528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.