MD09G1029900.v1.1

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000338
Physical Location & Seq
Reverse (-)
1836405 .. 1841846
5442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1029900.v1.1.491

Sequence Viewer

Length: 1803 bp
ATGGATGGTGAATACAGTAATGATTTTGACTGGAATACTGATGATGAGTTGGAAATTGAGAACTTTACGTTGTCTACTTCAAGTTTGACAGTTGGTGGAGAAGCTATTGTGATATCTGGGGAGGGGAGCTCATCTGCCGGTCCCTCCAATACCAAAGTTCTTGATCACTTTGTGGGGATGGGATTTTCTGAAAAGATGGTTGTCAAAGCAATTCAGGAACATGGAGAAGAAAACACAGATTCAATTCTGGAAACCCTCCTCACATATTCAGCTCTTGAGAGTTCCCCTGAAGAACAGCAGGAAGTAGAAACGCCGCAAGAAGTAGAAACGCCGCAGGAAGTAGAAACACCGCAGGAAGTAGAACCGCATGAAGTTGATTCCGATGACTTCTTTCTCGATGATTACGATGAGAGCTTTCTGGATGATTTTTCAGACTTGGATGGCTTTTCTGATACCGAGGAAATCATAAATCCTACATCGGAGGAAGGGAAGATACGGACCTTGGTCAATATGGGGTACACAAGGGAGGAGGCCTCAATAGCAATTGAAAGATGTGGTACAGACTCCACTGTTGCTGAGTTGACTGATTTTATATGTGCTGCTCAAATGGCACGGACGGAAGATGTCTTTTTTGCGGTTGAAGAGAAGCCTAGATCACATCTAGACAAGAAGAGGAAACTTCTTGAGTTTGAGATGCTAAAGAAGAAAAAGAAGCAGATGCGGCTTGGTGGAAACCAAGGGGATGATGATGAGACAGTTCATCTCCCAAATCCAATGGTTGGGTTTGGCACCCCAAGTGATCCATATGTGCTGACACATAGGACTCTTCCAGAGGCAGCTGTAGGACCTCCATACTTCTACTATGAGAACGTCGCACTAGCTCCTAAAGGGGTTTGGACCACCATCTCCCGCTTCCTGTATGATGTGCAGCCGGAGTTTGTGGACTCAAAGTACTTTTGTGCTGCTGCAAGGAAGAGAGGTTATGTCCACAATCTCCCAATCCAGAACAGGTTTCCTCTCATGCCACAGCCCCCAGACGCCATATCCAAGGCATTTCCTTTGACAAGGAAATGGTGGCCTTCTTGGGACAAGCGAGAAAAGCTAAACTGCTTGCAAACGTGTATTGCGAGTGCCAAGTTGACTGAGAGGATCAGGAAGGCTCTTGAGCAATATGATGGGGAACCACCAGAGCGAGTCCAGAAGTATGTCCTTGATGAATGCCGCAAGTGGAATCTGGTGTGGGTAGGAAGAAACAAGGTCGCCCCACTCGAGCCTGATGAGGTGGAGATGCTTTTGGGATTCCCAAAGAACCATACAAGGGGAATAAGCCGGACTGACAGATACAAGTCACTTGGGAACTCCTTCCAGGTTGACACTGTGGCATATCATCTGTCTGTGCTCAAGGATATGTTTCCCAGTGGCATCAACGTCCTCTCCCTCTTTACTGGCATTGGTGGGGCTGAAGTGGCCCTTCACCGGCTTGGTGTCCCTCTGAAGAACGTTGTCTCCGTAGAGATATCAGAAGTGAACAGAAACGTTGTGAGCAGTTGGTGGGAGCAAACCAACCAGAAGGGGAGGTTGCAGCACCTTGTCGACGTGCAGCAGCTGAATGGCGATCGCCTTGAGCAGTACATGAGCGAGTTCGGTGGATTTGATCTGGTGGTTGGTGGGAGTCCGTGCAACAATCTAGCAGGCAGCAACCGGCACCATCGTGATGGACTGGAGGGTAAGGAGTCTTCCCTTTTTTACGACTACTTCCGGATATTGGACTTAGTTAAGAGCATGATGGGAAGAAACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

601

Amino Acids

67.85

Weight (kDa)

4.77

Isoelectric Point (pI)

40.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 476 - 590 4.1e-09 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 788, 1704
AccB7I CCANNNNNTGG 1 cut(s) 779
AccI GTMKAC 2 cut(s) 74, 1593
AccIII TCCGGA 1 cut(s) 1758
AciI CCGC 8 cut(s) 314, 332, 350, 365, 635, 721, 910, 1222
AclI AACGTT 2 cut(s) 1500, 1536
AclWI GGATC 2 cut(s) 794, 1157
AcuI CTGAAG 3 cut(s) 309, 1482, 1514
AcyI GRCGYC 1 cut(s) 1038
AdeI CACNNNGTG 1 cut(s) 172
AfaI GTAC 4 cut(s) 518, 559, 953, 1631
AfiI CCNNNNNNNGG 4 cut(s) 779, 1318, 1476, 1765
AflIII ACRYGT 1 cut(s) 1118
AgsI TTSAA 4 cut(s) 81, 243, 548, 641
AjiI CACGTC 1 cut(s) 1597
AjnI CCWGG 1 cut(s) 1365
AleI CACNNNNGTG 1 cut(s) 1710
AloI GAACNNNNNNTCC 2 cut(s) 1490, 1522
AluBI AGCT 8 cut(s) 104, 129, 272, 414, 839, 881, 1102, 1606
AluI AGCT 8 cut(s) 104, 129, 272, 414, 839, 881, 1102, 1606
Alw21I GWGCWC 2 cut(s) 131, 1401
Alw26I GTCTC 2 cut(s) 746, 1510
AlwI GGATC 2 cut(s) 794, 1157
AlwNI CAGNNNCTG 1 cut(s) 1606
Ama87I CYCGRG 1 cut(s) 1268
Aor13HI TCCGGA 1 cut(s) 1758
AoxI GGCC 3 cut(s) 531, 1076, 1467
ApeKI GCWGC 9 cut(s) 599, 836, 928, 962, 965, 1582, 1600, 1603, 1695
AsiSI GCGATCGC 1 cut(s) 1618
Asp700I GAANNNNTTC 1 cut(s) 1361
AspS9I GGNCC 5 cut(s) 140, 498, 845, 897, 1468
AsuHPI GGTGA 2 cut(s) 20, 1466
AvaI CYCGRG 1 cut(s) 1268
AvaII GGWCC 4 cut(s) 140, 498, 845, 897
BanI GGYRCC 2 cut(s) 788, 1704
BanII GRGCYC 1 cut(s) 131
BarI GAAGNNNNNNTAC 4 cut(s) 477, 509, 1720, 1752
BbsI GAAGAC 1 cut(s) 1728
Bbv12I GWGCWC 2 cut(s) 131, 1401
BbvI GCAGC 9 cut(s) 586, 848, 940, 949, 952, 1594, 1612, 1615, 1707
BccI CCATC 8 cut(s) 172, 190, 434, 911, 1169, 1709, 1716, 1780
BciT130I CCWGG 1 cut(s) 1367
BclI TGATCA 1 cut(s) 163
BcoDI GTCTC 2 cut(s) 746, 1510
BfaI CTAG 4 cut(s) 651, 662, 878, 1688
BfmI CTRYAG 1 cut(s) 840
BmcAI AGTACT 1 cut(s) 953
Bme1390I CCNGG 1 cut(s) 1367
Bme18I GGWCC 4 cut(s) 140, 498, 845, 897
BmeT110I CYCGRG 1 cut(s) 1268
BmgBI CACGTC 1 cut(s) 1597
BmgT120I GGNCC 5 cut(s) 140, 498, 845, 897, 1468
BmiI GGNNCC 4 cut(s) 142, 790, 1182, 1706
BmrFI CCNGG 1 cut(s) 1367
BmrI ACTGGG 1 cut(s) 1410
BmsI GCATC 4 cut(s) 684, 708, 1278, 1431
BmuI ACTGGG 1 cut(s) 1410
BoxI GACNNNNGTC 1 cut(s) 503
BpiI GAAGAC 1 cut(s) 1728
BplI GAGNNNNNCTC 4 cut(s) 113, 145, 518, 550
BpmI CTGGAG 1 cut(s) 1742
BpuEI CTTGAG 5 cut(s) 296, 704, 1184, 1385, 1643
BsaHI GRCGYC 1 cut(s) 1038
BsaJI CCNNGG 4 cut(s) 456, 501, 736, 1047
BsaWI WCCGGW 1 cut(s) 1758
BsaXI ACNNNNNCTCC 4 cut(s) 1418, 1448, 1490, 1520
Bsc4I CCNNNNNNNGG 4 cut(s) 779, 1318, 1476, 1765
Bse118I RCCGGY 3 cut(s) 137, 1476, 1701
Bse1I ACTGG 4 cut(s) 35, 1416, 1450, 1725
BseAI TCCGGA 1 cut(s) 1758
BseBI CCWGG 1 cut(s) 1367
BseDI CCNNGG 4 cut(s) 456, 501, 736, 1047
BseGI GGATG 5 cut(s) 10, 183, 427, 445, 748
BseLI CCNNNNNNNGG 4 cut(s) 779, 1318, 1476, 1765
BseMII CTCAG 2 cut(s) 567, 1134
BseNI ACTGG 4 cut(s) 35, 1416, 1450, 1725
BseRI GAGGAG 2 cut(s) 248, 542
BseXI GCAGC 9 cut(s) 586, 848, 940, 949, 952, 1594, 1612, 1615, 1707
BsgI GTGCAG 2 cut(s) 947, 1619
Bsh1285I CGRYCG 1 cut(s) 1618
BshFI GGCC 3 cut(s) 533, 1078, 1469
BshNI GGYRCC 2 cut(s) 788, 1704
BsiEI CGRYCG 1 cut(s) 1618
BsiHKAI GWGCWC 2 cut(s) 131, 1401
BsiHKCI CYCGRG 1 cut(s) 1268
BsiSI CCGG 6 cut(s) 138, 932, 1330, 1477, 1702, 1759
BslFI GGGAC 3 cut(s) 126, 1100, 1472
BslI CCNNNNNNNGG 4 cut(s) 779, 1318, 1476, 1765
BsmAI GTCTC 2 cut(s) 746, 1510
BsmFI GGGAC 3 cut(s) 126, 1100, 1472
BsmI GAATGC 1 cut(s) 1223
BsnI GGCC 3 cut(s) 533, 1078, 1469
BsoBI CYCGRG 1 cut(s) 1268
Bsp1286I GDGCHC 2 cut(s) 131, 1401
Bsp13I TCCGGA 1 cut(s) 1758
Bsp143I GATC 6 cut(s) 163, 653, 799, 1149, 1615, 1654
BspACI CCGC 8 cut(s) 314, 332, 350, 365, 635, 721, 910, 1222
BspANI GGCC 3 cut(s) 533, 1078, 1469
BspCNI CTCAG 2 cut(s) 568, 1135
BspEI TCCGGA 1 cut(s) 1758
BspLI GGNNCC 4 cut(s) 142, 790, 1182, 1706
BspPI GGATC 2 cut(s) 794, 1157
BspT107I GGYRCC 2 cut(s) 788, 1704
BsrFI RCCGGY 3 cut(s) 137, 1476, 1701
BsrI ACTGG 4 cut(s) 35, 1416, 1450, 1725
BssAI RCCGGY 3 cut(s) 137, 1476, 1701
BssECI CCNNGG 4 cut(s) 456, 501, 736, 1047
BssMI GATC 6 cut(s) 163, 653, 799, 1149, 1615, 1654
BssNI GRCGYC 1 cut(s) 1038
BssT1I CCWWGG 3 cut(s) 501, 736, 1047
Bst2UI CCWGG 1 cut(s) 1367
Bst4CI ACNGT 5 cut(s) 17, 91, 571, 757, 1378
Bst6I CTCTTC 4 cut(s) 636, 665, 831, 968
BstACI GRCGYC 1 cut(s) 1038
BstC8I GCNNGC 2 cut(s) 1112, 1693
BstDEI CTNAG 3 cut(s) 576, 1143, 1771
BstF5I GGATG 5 cut(s) 10, 183, 427, 445, 748
BstKTI GATC 6 cut(s) 166, 656, 802, 1152, 1618, 1657
BstMAI GTCTC 2 cut(s) 746, 1510
BstMBI GATC 6 cut(s) 163, 653, 799, 1149, 1615, 1654
BstMCI CGRYCG 1 cut(s) 1618
BstMWI GCNNNNNNNGC 5 cut(s) 539, 608, 721, 1099, 1466
BstNI CCWGG 1 cut(s) 1367
BstPAI GACNNNNGTC 1 cut(s) 503
BstSCI CCNGG 1 cut(s) 1365
BstSFI CTRYAG 1 cut(s) 840
BstV1I GCAGC 9 cut(s) 586, 848, 940, 949, 952, 1594, 1612, 1615, 1707
BstV2I GAAGAC 1 cut(s) 1728
BstXI CCANNNNNNTGG 1 cut(s) 1715
BsuRI GGCC 3 cut(s) 533, 1078, 1469
BtrI CACGTC 1 cut(s) 1597
BtsCI GGATG 5 cut(s) 10, 183, 427, 445, 748
BtsIMutI CAGTG 3 cut(s) 567, 1374, 1423
Cac8I GCNNGC 2 cut(s) 1112, 1693
CaiI CAGNNNCTG 1 cut(s) 1606
Cfr10I RCCGGY 3 cut(s) 137, 1476, 1701
Cfr13I GGNCC 5 cut(s) 140, 498, 845, 897, 1468
CseI GACGC 1 cut(s) 1046
Csp6I GTAC 4 cut(s) 517, 558, 952, 1630
CviAII CATG 5 cut(s) 221, 368, 1021, 1633, 1783
CviQI GTAC 4 cut(s) 517, 558, 952, 1630
DdeI CTNAG 3 cut(s) 576, 1143, 1771
DpnI GATC 6 cut(s) 165, 655, 801, 1151, 1617, 1656
DpnII GATC 6 cut(s) 163, 653, 799, 1149, 1615, 1654
DraIII CACNNNGTG 1 cut(s) 172
Eam1104I CTCTTC 4 cut(s) 636, 665, 831, 968
EarI CTCTTC 4 cut(s) 636, 665, 831, 968
Ecl136II GAGCTC 1 cut(s) 129
Eco130I CCWWGG 3 cut(s) 501, 736, 1047
Eco147I AGGCCT 1 cut(s) 533
Eco24I GRGCYC 1 cut(s) 131
Eco32I GATATC 2 cut(s) 114, 1518
Eco47I GGWCC 4 cut(s) 140, 498, 845, 897
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 3 cut(s) 309, 1482, 1514
Eco88I CYCGRG 1 cut(s) 1268
EcoICRI GAGCTC 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 845
EcoRII CCWGG 1 cut(s) 1365
EcoRV GATATC 2 cut(s) 114, 1518
EcoT14I CCWWGG 3 cut(s) 501, 736, 1047
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 3 cut(s) 501, 736, 1047
FaeI CATG 5 cut(s) 224, 371, 1024, 1636, 1786
FalI AAGNNNNNCTT 2 cut(s) 1455, 1487
FaqI GGGAC 3 cut(s) 126, 1100, 1472
FatI CATG 5 cut(s) 220, 367, 1020, 1632, 1782
FauI CCCGC 1 cut(s) 917
FauNDI CATATG 1 cut(s) 805
FbaI TGATCA 1 cut(s) 163
FblI GTMKAC 2 cut(s) 74, 1593
FokI GGATG 5 cut(s) 17, 190, 434, 452, 755
FriOI GRGCYC 1 cut(s) 131
FspBI CTAG 4 cut(s) 651, 662, 878, 1688
GsuI CTGGAG 1 cut(s) 1742
HaeIII GGCC 3 cut(s) 533, 1078, 1469
HapII CCGG 6 cut(s) 138, 932, 1330, 1477, 1702, 1759
HgaI GACGC 1 cut(s) 1046
Hin1I GRCGYC 1 cut(s) 1038
Hin1II CATG 5 cut(s) 224, 371, 1024, 1636, 1786
HincII GTYRAC 4 cut(s) 582, 1140, 1372, 1594
HindII GTYRAC 4 cut(s) 582, 1140, 1372, 1594
HpaII CCGG 6 cut(s) 138, 932, 1330, 1477, 1702, 1759
HphI GGTGA 2 cut(s) 20, 1466
Hpy166II GTNNAC 9 cut(s) 75, 519, 582, 943, 988, 1140, 1372, 1528, 1594
Hpy188I TCNGA 7 cut(s) 190, 382, 433, 451, 481, 1494, 1522
Hpy8I GTNNAC 9 cut(s) 75, 519, 582, 943, 988, 1140, 1372, 1528, 1594
Hpy99I CGWCG 2 cut(s) 875, 1598
HpyAV CCTTC 6 cut(s) 479, 1089, 1150, 1372, 1481, 1564
HpyCH4III ACNGT 5 cut(s) 17, 91, 571, 757, 1378
HpyCH4IV ACGT 7 cut(s) 68, 870, 1118, 1428, 1500, 1536, 1596
HpyCH4V TGCA 6 cut(s) 928, 968, 1114, 1582, 1600, 1680
HpyF10VI GCNNNNNNNGC 5 cut(s) 539, 608, 721, 1099, 1466
HpyF3I CTNAG 3 cut(s) 576, 1143, 1771
HpySE526I ACGT 7 cut(s) 68, 870, 1118, 1428, 1500, 1536, 1596
Hsp92I GRCGYC 1 cut(s) 1038
Hsp92II CATG 5 cut(s) 224, 371, 1024, 1636, 1786
Kpn2I TCCGGA 1 cut(s) 1758
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 6 cut(s) 163, 653, 799, 1149, 1615, 1654
LmnI GCTCC 3 cut(s) 126, 886, 1555
Lsp1109I GCAGC 9 cut(s) 586, 848, 940, 949, 952, 1594, 1612, 1615, 1707
LweI GCATC 4 cut(s) 684, 708, 1278, 1431
MaeI CTAG 4 cut(s) 651, 662, 878, 1688
MaeII ACGT 7 cut(s) 68, 870, 1118, 1428, 1500, 1536, 1596
MaeIII GTNAC 1 cut(s) 1347
MalI GATC 6 cut(s) 165, 655, 801, 1151, 1617, 1656
MboI GATC 6 cut(s) 163, 653, 799, 1149, 1615, 1654
MfeI CAATTG 2 cut(s) 543, 1798
MhlI GDGCHC 2 cut(s) 131, 1401
MluCI AATT 5 cut(s) 54, 210, 243, 543, 1798
MlyI GAGTC 6 cut(s) 557, 817, 938, 1203, 1681, 1742
MmeI TCCRAC 1 cut(s) 30
MroI TCCGGA 1 cut(s) 1758
MroXI GAANNNNTTC 1 cut(s) 1361
MseI TTAA 1 cut(s) 1776
MslI CAYNNNNRTG 2 cut(s) 1710, 1713
MspA1I CMGCKG 2 cut(s) 839, 1606
MspI CCGG 6 cut(s) 138, 932, 1330, 1477, 1702, 1759
MspR9I CCNGG 1 cut(s) 1367
MunI CAATTG 2 cut(s) 543, 1798
Mva1269I GAATGC 1 cut(s) 1223
MvaI CCWGG 1 cut(s) 1367
MwoI GCNNNNNNNGC 5 cut(s) 539, 608, 721, 1099, 1466
NdeI CATATG 1 cut(s) 805
NdeII GATC 6 cut(s) 163, 653, 799, 1149, 1615, 1654
NlaIII CATG 5 cut(s) 224, 371, 1024, 1636, 1786
NlaIV GGNNCC 4 cut(s) 142, 790, 1182, 1706
NmuCI GTSAC 1 cut(s) 1347
OliI CACNNNNGTG 1 cut(s) 1710
PaeR7I CTCGAG 1 cut(s) 1268
PceI AGGCCT 1 cut(s) 533
PcsI WCGNNNNNNNCGW 4 cut(s) 402, 1124, 1266, 1506
PctI GAATGC 1 cut(s) 1223
PdmI GAANNNNTTC 1 cut(s) 1361
PfeI GAWTC 4 cut(s) 239, 377, 1231, 1299
PflMI CCANNNNNTGG 1 cut(s) 779
Ple19I CGATCG 1 cut(s) 1618
PleI GAGTC 6 cut(s) 557, 817, 938, 1202, 1680, 1741
PpsI GAGTC 6 cut(s) 557, 817, 938, 1202, 1680, 1741
PpuMI RGGWCCY 1 cut(s) 845
PshAI GACNNNNGTC 1 cut(s) 503
Psp124BI GAGCTC 1 cut(s) 131
Psp1406I AACGTT 2 cut(s) 1500, 1536
Psp5II RGGWCCY 1 cut(s) 845
Psp6I CCWGG 1 cut(s) 1365
PspGI CCWGG 1 cut(s) 1365
PspN4I GGNNCC 4 cut(s) 142, 790, 1182, 1706
PspPI GGNCC 5 cut(s) 140, 498, 845, 897, 1468
PspPPI RGGWCCY 1 cut(s) 845
PspXI VCTCGAGB 1 cut(s) 1268
PstNI CAGNNNCTG 1 cut(s) 1606
PvuI CGATCG 1 cut(s) 1618
PvuII CAGCTG 2 cut(s) 839, 1606
RgaI GCGATCGC 1 cut(s) 1618
RsaI GTAC 4 cut(s) 518, 559, 953, 1631
RsaNI GTAC 4 cut(s) 517, 558, 952, 1630
RseI CAYNNNNRTG 2 cut(s) 1710, 1713
SacI GAGCTC 1 cut(s) 131
SalI GTCGAC 1 cut(s) 1592
SaqAI TTAA 1 cut(s) 1776
Sau3AI GATC 6 cut(s) 163, 653, 799, 1149, 1615, 1654
Sau96I GGNCC 5 cut(s) 140, 498, 845, 897, 1468
ScaI AGTACT 1 cut(s) 953
SchI GAGTC 6 cut(s) 557, 817, 938, 1203, 1681, 1742
ScrFI CCNGG 1 cut(s) 1367
SduI GDGCHC 2 cut(s) 131, 1401
SfaAI GCGATCGC 1 cut(s) 1618
SfaNI GCATC 4 cut(s) 684, 708, 1278, 1431
SfcI CTRYAG 1 cut(s) 840
Sfr274I CTCGAG 1 cut(s) 1268
SgfI GCGATCGC 1 cut(s) 1618
SinI GGWCC 4 cut(s) 140, 498, 845, 897
SlaI CTCGAG 1 cut(s) 1268
SmiMI CAYNNNNRTG 2 cut(s) 1710, 1713
SmlI CTYRAG 6 cut(s) 275, 683, 1163, 1268, 1400, 1622
SmoI CTYRAG 6 cut(s) 275, 683, 1163, 1268, 1400, 1622
Sse9I AATT 5 cut(s) 54, 210, 243, 543, 1798
SseBI AGGCCT 1 cut(s) 533
SsiI CCGC 8 cut(s) 314, 332, 350, 365, 635, 721, 910, 1222
SspMI CTAG 4 cut(s) 651, 662, 878, 1688
SstI GAGCTC 1 cut(s) 131
StuI AGGCCT 1 cut(s) 533
StyD4I CCNGG 1 cut(s) 1365
StyI CCWWGG 3 cut(s) 501, 736, 1047
TaaI ACNGT 5 cut(s) 17, 91, 571, 757, 1378
TaiI ACGT 7 cut(s) 71, 873, 1121, 1431, 1503, 1539, 1599
TaqI TCGA 3 cut(s) 396, 1269, 1593
TasI AATT 5 cut(s) 54, 210, 243, 543, 1798
TatI WGTACW 2 cut(s) 951, 1629
TauI GCSGC 4 cut(s) 316, 334, 724, 1224
TfiI GAWTC 4 cut(s) 239, 377, 1231, 1299
Tru1I TTAA 1 cut(s) 1776
Tru9I TTAA 1 cut(s) 1776
TscAI CASTG 3 cut(s) 574, 1381, 1423
TseFI GTSAC 1 cut(s) 1347
TseI GCWGC 9 cut(s) 599, 836, 928, 962, 965, 1582, 1600, 1603, 1695
Tsp45I GTSAC 1 cut(s) 1347
TspDTI ATGAA 3 cut(s) 384, 749, 1230
TspGWI ACGGA 5 cut(s) 511, 628, 632, 1498, 1665
TspRI CASTG 3 cut(s) 574, 1381, 1423
Van91I CCANNNNNTGG 1 cut(s) 779
VpaK11BI GGWCC 4 cut(s) 140, 498, 845, 897
XbaI TCTAGA 1 cut(s) 661
XhoI CTCGAG 1 cut(s) 1268
XmiI GTMKAC 2 cut(s) 74, 1593
XmnI GAANNNNTTC 1 cut(s) 1361
XspI CTAG 4 cut(s) 651, 662, 878, 1688
ZrmI AGTACT 1 cut(s) 953
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.