Rorug01G0036600

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000338
Physical Location & Seq
Reverse (-)
6108911 .. 6109757
847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0036600.1

Sequence Viewer

Length: 798 bp
ATGGCAGTGGGAGAGATCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTGGGGAGATCCTTGGCTACTTTGGAAGCTTGGGAGGTGTTGGCAAAGAGCTGCAGAAATGGAGGGGAACACTGTCCGCAATTGCAGCGGTGCTTTCCGATGCGGAGGAGAAGCAGCTGACGAGCAAGGCAGTGGATCTGTGGCTGAATGACCTCAAACACTTGGCTTATGATCTGGACGATCTACTCAACACCTTTTCTAATGAGATGTTAGAACGCGAGCAGCTCAAGCTTCACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCACAAAGTTAAGTTCAATTTCAATATGAACTCCGAAATAGACGAGATTGCTTCTCGGACACCAAGTTCATATGTGTTGGATGGACCTGTGGTTGGGAGAGATGAAGACACAAGAAAGATTGTGGAATTGTTGTCCAGAGGTGTTGATCCTTCTTCTCCCACAAATTATCAAGTTGTTGCCATTGTTGGTATGGGTGGACTCGGCAAGACAACGCTTGCGGGACAAGTCTTCAATGATGTAGCTGCAATGGAACAGTTTGATCTCAAGGTCTGGGTTTCAGTGTCTGATGACTTCGATCTTCAAACGGTGACGAGAGCTATTTTTAAGGAAGTTACATCTCGGCCTTGTGATATGGATGAGTTCAGTCGACTTCAAGATAATCTTGTAAGAATTAATGTGGACTTATCAATGTTCAGTAAGGACCACAACGACTTAGCTACCTTATTTGCAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

265

Amino Acids

29.49

Weight (kDa)

4.84

Isoelectric Point (pI)

26.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 99 2.1e-19 Rx N-terminal domain
NB-ARC PF00931 152 - 241 8.1e-17 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 709
AccII CGCG 1 cut(s) 279
AciI CCGC 5 cut(s) 26, 138, 149, 164, 560
AclWI GGATC 3 cut(s) 64, 204, 482
AcuI CTGAAG 1 cut(s) 20
AfaI GTAC 1 cut(s) 318
AfiI CCNNNNNNNGG 1 cut(s) 434
AgsI TTSAA 5 cut(s) 358, 364, 574, 644, 716
AjnI CCWGG 1 cut(s) 61
AloI GAACNNNNNNTCC 2 cut(s) 391, 423
AluBI AGCT 8 cut(s) 90, 112, 178, 286, 292, 584, 659, 779
AluI AGCT 8 cut(s) 90, 112, 178, 286, 292, 584, 659, 779
AlwI GGATC 3 cut(s) 64, 204, 482
AlwNI CAGNNNCTG 2 cut(s) 43, 626
AoxI GGCC 1 cut(s) 683
ApeKI GCWGC 6 cut(s) 43, 112, 146, 175, 283, 584
AseI ATTAAT 1 cut(s) 735
AspS9I GGNCC 2 cut(s) 425, 763
AsuHPI GGTGA 2 cut(s) 287, 661
AvaII GGWCC 2 cut(s) 425, 763
BbsI GAAGAC 2 cut(s) 453, 562
BbvI GCAGC 6 cut(s) 30, 99, 158, 187, 295, 571
BccI CCATC 1 cut(s) 416
BciT130I CCWGG 1 cut(s) 63
BfmI CTRYAG 1 cut(s) 113
BglII AGATCT 1 cut(s) 15
BisI GCNGC 7 cut(s) 27, 44, 113, 147, 176, 284, 585
BlsI GCNGC 7 cut(s) 28, 45, 114, 148, 177, 285, 586
Bme1390I CCNGG 1 cut(s) 63
Bme18I GGWCC 2 cut(s) 425, 763
BmgT120I GGNCC 2 cut(s) 425, 763
BmrFI CCNGG 1 cut(s) 63
BmsI GCATC 1 cut(s) 151
BpiI GAAGAC 2 cut(s) 453, 562
BpuEI CTTGAG 2 cut(s) 272, 590
BsaJI CCNNGG 2 cut(s) 62, 73
Bsc4I CCNNNNNNNGG 1 cut(s) 434
Bse1I ACTGG 1 cut(s) 307
Bse3DI GCAATG 1 cut(s) 594
BseBI CCWGG 1 cut(s) 63
BseDI CCNNGG 2 cut(s) 62, 73
BseGI GGATG 2 cut(s) 427, 703
BseLI CCNNNNNNNGG 1 cut(s) 434
BseMI GCAATG 1 cut(s) 594
BseNI ACTGG 1 cut(s) 307
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 6 cut(s) 30, 99, 158, 187, 295, 571
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 1 cut(s) 685
BslFI GGGAC 1 cut(s) 576
BslI CCNNNNNNNGG 1 cut(s) 434
BsmFI GGGAC 1 cut(s) 576
BsmI GAATGC 1 cut(s) 29
BsnI GGCC 1 cut(s) 685
Bsp143I GATC 8 cut(s) 15, 69, 196, 232, 241, 487, 601, 637
BspACI CCGC 5 cut(s) 26, 138, 149, 164, 560
BspANI GGCC 1 cut(s) 685
BspFNI CGCG 1 cut(s) 279
BspMAI CTGCAG 1 cut(s) 117
BspPI GGATC 3 cut(s) 64, 204, 482
BsrDI GCAATG 1 cut(s) 594
BsrI ACTGG 1 cut(s) 307
BssECI CCNNGG 2 cut(s) 62, 73
BssMI GATC 8 cut(s) 15, 69, 196, 232, 241, 487, 601, 637
BssT1I CCWWGG 1 cut(s) 73
Bst2UI CCWGG 1 cut(s) 63
Bst4CI ACNGT 3 cut(s) 135, 597, 649
BstC8I GCNNGC 2 cut(s) 281, 558
BstDEI CTNAG 1 cut(s) 775
BstF5I GGATG 2 cut(s) 427, 703
BstFNI CGCG 1 cut(s) 279
BstKTI GATC 8 cut(s) 18, 72, 199, 235, 244, 490, 604, 640
BstMBI GATC 8 cut(s) 15, 69, 196, 232, 241, 487, 601, 637
BstMWI GCNNNNNNNGC 2 cut(s) 146, 289
BstNI CCWGG 1 cut(s) 63
BstSCI CCNGG 1 cut(s) 61
BstSFI CTRYAG 1 cut(s) 113
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 6 cut(s) 30, 99, 158, 187, 295, 571
BstV2I GAAGAC 2 cut(s) 453, 562
BstX2I RGATCY 3 cut(s) 15, 69, 196
BstYI RGATCY 3 cut(s) 15, 69, 196
BsuRI GGCC 1 cut(s) 685
BtsCI GGATG 2 cut(s) 427, 703
BtsI GCAGTG 2 cut(s) 12, 198
BtsIMutI CAGTG 4 cut(s) 12, 131, 198, 627
Cac8I GCNNGC 2 cut(s) 281, 558
CaiI CAGNNNCTG 2 cut(s) 43, 626
Cfr13I GGNCC 2 cut(s) 425, 763
Csp6I GTAC 1 cut(s) 317
CviQI GTAC 1 cut(s) 317
DdeI CTNAG 1 cut(s) 775
DpnI GATC 8 cut(s) 17, 71, 198, 234, 243, 489, 603, 639
DpnII GATC 8 cut(s) 15, 69, 196, 232, 241, 487, 601, 637
Eco130I CCWWGG 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 425, 763
Eco57I CTGAAG 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 73
FaiI YATR 6 cut(s) 231, 368, 412, 414, 533, 695
FalI AAGNNNNNCTT 2 cut(s) 708, 740
FaqI GGGAC 1 cut(s) 576
FauI CCCGC 1 cut(s) 553
FauNDI CATATG 1 cut(s) 412
FblI GTMKAC 1 cut(s) 709
Fnu4HI GCNGC 7 cut(s) 27, 44, 113, 147, 176, 284, 585
FokI GGATG 2 cut(s) 434, 710
Fsp4HI GCNGC 7 cut(s) 27, 44, 113, 147, 176, 284, 585
GluI GCNGC 7 cut(s) 27, 44, 113, 147, 176, 284, 585
HaeIII GGCC 1 cut(s) 685
HincII GTYRAC 2 cut(s) 57, 710
HindII GTYRAC 2 cut(s) 57, 710
HindIII AAGCTT 2 cut(s) 88, 290
HinfI GANTC 1 cut(s) 540
HpaI GTTAAC 1 cut(s) 57
HphI GGTGA 2 cut(s) 287, 661
Hpy166II GTNNAC 4 cut(s) 57, 539, 710, 742
Hpy188I TCNGA 4 cut(s) 160, 376, 399, 628
Hpy188III TCNNGA 3 cut(s) 236, 477, 716
Hpy8I GTNNAC 4 cut(s) 57, 539, 710, 742
HpyAV CCTTC 2 cut(s) 44, 501
HpyCH4III ACNGT 3 cut(s) 135, 597, 649
HpyCH4V TGCA 4 cut(s) 115, 146, 587, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 289
HpyF3I CTNAG 1 cut(s) 775
KspAI GTTAAC 1 cut(s) 57
Kzo9I GATC 8 cut(s) 15, 69, 196, 232, 241, 487, 601, 637
LpnPI CCDG 9 cut(s) 32, 37, 48, 75, 221, 288, 441, 490, 598
Lsp1109I GCAGC 6 cut(s) 30, 99, 158, 187, 295, 571
LweI GCATC 1 cut(s) 151
MaeIII GTNAC 2 cut(s) 649, 673
MalI GATC 8 cut(s) 17, 71, 198, 234, 243, 489, 603, 639
MboI GATC 8 cut(s) 15, 69, 196, 232, 241, 487, 601, 637
MboII GAAGA 4 cut(s) 458, 486, 562, 632
MfeI CAATTG 1 cut(s) 141
MflI RGATCY 3 cut(s) 15, 69, 196
MluCI AATT 5 cut(s) 141, 358, 467, 505, 732
MlyI GAGTC 1 cut(s) 534
MmeI TCCRAC 1 cut(s) 399
MnlI CCTC 7 cut(s) 89, 117, 160, 224, 314, 351, 473
MseI TTAA 4 cut(s) 56, 351, 666, 735
MspA1I CMGCKG 2 cut(s) 149, 178
MspR9I CCNGG 1 cut(s) 63
MunI CAATTG 1 cut(s) 141
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 1 cut(s) 63
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 2 cut(s) 146, 289
NdeI CATATG 1 cut(s) 412
NdeII GATC 8 cut(s) 15, 69, 196, 232, 241, 487, 601, 637
NmeAIII GCCGAG 2 cut(s) 522, 661
NmuCI GTSAC 1 cut(s) 649
PctI GAATGC 1 cut(s) 29
PkrI GCNGC 7 cut(s) 28, 45, 114, 148, 177, 285, 586
PleI GAGTC 1 cut(s) 534
PpsI GAGTC 1 cut(s) 534
PshBI ATTAAT 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 61
PspGI CCWGG 1 cut(s) 61
PspPI GGNCC 2 cut(s) 425, 763
PstI CTGCAG 1 cut(s) 117
PstNI CAGNNNCTG 2 cut(s) 43, 626
PsuI RGATCY 3 cut(s) 15, 69, 196
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 1 cut(s) 318
RsaNI GTAC 1 cut(s) 317
SalI GTCGAC 1 cut(s) 708
SaqAI TTAA 4 cut(s) 56, 351, 666, 735
SatI GCNGC 7 cut(s) 27, 44, 113, 147, 176, 284, 585
Sau3AI GATC 8 cut(s) 15, 69, 196, 232, 241, 487, 601, 637
Sau96I GGNCC 2 cut(s) 425, 763
SchI GAGTC 1 cut(s) 534
ScrFI CCNGG 1 cut(s) 63
SfaNI GCATC 1 cut(s) 151
SfcI CTRYAG 1 cut(s) 113
SinI GGWCC 2 cut(s) 425, 763
SmlI CTYRAG 2 cut(s) 287, 605
SmoI CTYRAG 2 cut(s) 287, 605
Sse9I AATT 5 cut(s) 141, 358, 467, 505, 732
SsiI CCGC 5 cut(s) 26, 138, 149, 164, 560
StyD4I CCNGG 1 cut(s) 61
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 3 cut(s) 135, 597, 649
TaqI TCGA 2 cut(s) 636, 709
TasI AATT 5 cut(s) 141, 358, 467, 505, 732
TauI GCSGC 1 cut(s) 29
Tru1I TTAA 4 cut(s) 56, 351, 666, 735
Tru9I TTAA 4 cut(s) 56, 351, 666, 735
TscAI CASTG 4 cut(s) 12, 138, 198, 627
TseFI GTSAC 1 cut(s) 649
TseI GCWGC 6 cut(s) 43, 112, 146, 175, 283, 584
Tsp45I GTSAC 1 cut(s) 649
TspDTI ATGAA 3 cut(s) 383, 399, 459
TspRI CASTG 4 cut(s) 12, 138, 198, 627
VpaK11BI GGWCC 2 cut(s) 425, 763
VspI ATTAAT 1 cut(s) 735
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XmiI GTMKAC 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.