FvH4_6g50980

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000338
Physical Location & Seq
Forward (+)
37853381 .. 37859526
6146 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50980.t1

Sequence Viewer

Length: 1791 bp
ATGGATGGAAATACATCTGGGGTAAATGGAAATAATGTTGACTGGGATACTGAAGATGAGCTTGACGAGATTGAAAATTTCACTTTATCGTCTTCAGCTAATCTGTGCTCTGGGGAGGGGAGCTCATCTCCAGGACCTTCCAATACCAAAGTTTTTGGTCATTTTGTCAGCATGGGGTTTTCTCACAAAATGGTTTCCAAAGCTGTAAAGGAACATGGTGAGGAAGACGCAGATTCAATTCTGGAAAGCCTCCTCTTATATAATGCTCTTGAAAATTCAACTTCAGAGCAGGATTCTCCTCCAGAGCAGGATTCTCCACCAGAACAGTTTTTTCCTCCACAACAGCAACAAATTGATTCTGATCCCTTTTCTTCAGATTATGAGGGGAGCTTTCTGGATGGATTTTCAGATTTAGATAGCTCCGATAATGAGGAAAGTTTCATTCCAACATCTGAGGAGGATAAAAAACTTGTGTCCTTGGTCAATATGGGGTACACAGAATTCGAGGCTTCAATCGCAATAGAAAGATGCGGTGTAGACTCATCCCTGGTTGATTTGACTGATTTCATATCAGCTGCTCAGTTTTCAAAGGCAGAAGATGTCCATTTTCCCCTTGAAGAGAAGCCTAGACGTCTTCACATTGATCACAAGAAGAAAAGGAAACTCTGTGAATATGCCATGTCGAAAAGAAAAAAGAAGGTGGGACTTGGAAATGGGATCATTGAAGAAGATGATGAAGCACTTCATCTCCCAAATCCAATGATTGGATTTGGGACTCCAACACATCTTTGTCAAGTCCACAGGAGTATACCAGAGGCAGCCAGAGGACCTCCTTACTTCTACTATGAAAATGTGGCATTGACTCCTAAAGGGGTTTGGAACACAATTTCCAGATTTCTGTATGATATTCCACCTGAGTTTGTTGATTCCAAGTATTTCTGTGCAGCGGCTAGGAAAAGAGGTTATATTCATAATCTCCCAATAGAGAATAGGTTTCCTCTTATTCCTCTCCCACCGCAGAATATATTTGATGCATTTCCCATGACAAGGAGATGGTGGCCTTGTTGGGACGAGCGCAGACAGCTGAACTGCCTACAAACTTGCTATGCTAGTGCACAGTTGACTGAGAGGATCAGACTGGCTCTGAAAGACTATGGTGAAGATGAGGTTCCACCAGAAAGAGTTCGACGGTATGTGCTACATGAATGCCGCAAGTGGAATCTTTTGTGGGTAGGAAAGAACAAGGTTGCCCCGTTGGAGCCTGATGAGGTGGAAATGCTTTTGGGATTCCCCAGGAACCATACAAGGGGGATAAGTAGGACTGATAGATATAAGTCACTTGGGAACTCATTTCAGATTGATACTGTAGCCTATCATCTCTCTGTGTTGAAAGATCGGTTTCCCAATGGCATCAATCTCCTCTCTCTTTTCTCTGGGATTGGCGGTGCCGAGATAGCCCTCCATCGGCTTGGCATACGGATGAAGAATGTTGTGTCCGTTGAGATATCAGGGGTGAGCAGAACCGTTGTAAGGACTTGGTGGGAGCAAACCAACCAAAAAGGAAACTTGTATCACCTTGCTGATGTGCAAGAGTTGAACGCCGACCGGTTGGAGCATTATATCAACATCTTTGGTGGTTTTGATCTTGTTGTTGGAGGGAGCCCGTGCAACAATCTTGCTGGTAGCAACAGACATCATAGGGATGGGCTAGAAGGGAAAGAATCTTCTCTGTTTTATGATTATTTTCGTATATTAGACATAGTTAAGGGTCTTATGACAACGAACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

597

Amino Acids

67.48

Weight (kDa)

5.21

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 472 - 587 3.8e-09 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 634
AccB1I GGYRCC 1 cut(s) 1445
AccB7I CCANNNNNTGG 1 cut(s) 764
AccI GTMKAC 2 cut(s) 537, 808
AciI CCGC 5 cut(s) 531, 947, 1016, 1210, 1443
AclWI GGATC 3 cut(s) 356, 725, 1139
AcsI RAATTY 3 cut(s) 76, 274, 500
AcuI CTGAAG 4 cut(s) 72, 78, 267, 357
AcyI GRCGYC 1 cut(s) 631
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 5 cut(s) 764, 1047, 1306, 1464, 1530
AgeI ACCGGT 1 cut(s) 1605
AjnI CCWGG 3 cut(s) 130, 546, 1292
AloI GAACNNNNNNTCC 2 cut(s) 872, 904
AluBI AGCT 8 cut(s) 61, 98, 123, 203, 390, 420, 575, 1084
AluI AGCT 8 cut(s) 61, 98, 123, 203, 390, 420, 575, 1084
Alw21I GWGCWC 3 cut(s) 110, 125, 1117
Alw44I GTGCAC 1 cut(s) 1113
AlwI GGATC 3 cut(s) 356, 725, 1139
AoxI GGCC 1 cut(s) 1058
ApaLI GTGCAC 1 cut(s) 1113
ApeKI GCWGC 3 cut(s) 575, 818, 944
ApoI RAATTY 3 cut(s) 76, 274, 500
AsiGI ACCGGT 1 cut(s) 1605
Asp700I GAANNNNTTC 2 cut(s) 741, 1182
AspLEI GCGC 1 cut(s) 1077
AspS9I GGNCC 2 cut(s) 134, 827
AsuHPI GGTGA 4 cut(s) 230, 1169, 1525, 1565
AvaII GGWCC 2 cut(s) 134, 827
BaeGI GKGCMC 1 cut(s) 1117
BanI GGYRCC 1 cut(s) 1445
BanII GRGCYC 2 cut(s) 125, 1664
BbsI GAAGAC 3 cut(s) 84, 231, 626
Bbv12I GWGCWC 3 cut(s) 110, 125, 1117
BbvI GCAGC 3 cut(s) 562, 830, 956
BccI CCATC 4 cut(s) 392, 1047, 1470, 1697
BciT130I CCWGG 3 cut(s) 132, 548, 1294
BciVI GTATCC 1 cut(s) 40
BclI TGATCA 1 cut(s) 643
BfaI CTAG 4 cut(s) 627, 951, 1110, 1709
BfmI CTRYAG 1 cut(s) 1365
BfuI GTATCC 1 cut(s) 40
BisI GCNGC 5 cut(s) 576, 819, 945, 948, 1210
BlsI GCNGC 5 cut(s) 577, 820, 946, 949, 1211
Bme1390I CCNGG 3 cut(s) 132, 548, 1294
Bme18I GGWCC 2 cut(s) 134, 827
BmgT120I GGNCC 2 cut(s) 134, 827
BmiI GGNNCC 5 cut(s) 1170, 1260, 1298, 1447, 1661
BmrFI CCNGG 3 cut(s) 132, 548, 1294
BmrI ACTGGG 1 cut(s) 52
BmsI GCATC 3 cut(s) 518, 1021, 1419
BmuI ACTGGG 1 cut(s) 52
BpiI GAAGAC 3 cut(s) 84, 231, 626
BplI GAGNNNNNCTC 4 cut(s) 107, 139, 112, 144
BpmI CTGGAG 2 cut(s) 114, 285
BsaBI GATNNNNATC 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 631
BsaJI CCNNGG 3 cut(s) 477, 546, 1292
BsaWI WCCGGW 1 cut(s) 1605
BsaXI ACNNNNNCTCC 2 cut(s) 732, 762
Bsc4I CCNNNNNNNGG 5 cut(s) 764, 1047, 1306, 1464, 1530
Bse118I RCCGGY 1 cut(s) 1605
Bse1I ACTGG 2 cut(s) 47, 1143
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 3 cut(s) 132, 548, 1294
BseDI CCNNGG 3 cut(s) 477, 546, 1292
BseGI GGATG 5 cut(s) 10, 403, 542, 1485, 1708
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 5 cut(s) 764, 1047, 1306, 1464, 1530
BseMII CTCAG 4 cut(s) 444, 593, 906, 1116
BseNI ACTGG 2 cut(s) 47, 1143
BseRI GAGGAG 4 cut(s) 242, 288, 470, 1409
BseSI GKGCMC 1 cut(s) 1117
BseXI GCAGC 3 cut(s) 562, 830, 956
BsgI GTGCAG 1 cut(s) 963
Bsh1285I CGRYCG 1 cut(s) 1606
BshFI GGCC 1 cut(s) 1060
BshNI GGYRCC 1 cut(s) 1445
BshTI ACCGGT 1 cut(s) 1605
BsiEI CGRYCG 1 cut(s) 1606
BsiHKAI GWGCWC 3 cut(s) 110, 125, 1117
BsiSI CCGG 1 cut(s) 1606
BslFI GGGAC 3 cut(s) 717, 787, 1082
BslI CCNNNNNNNGG 5 cut(s) 764, 1047, 1306, 1464, 1530
BsmFI GGGAC 3 cut(s) 717, 787, 1082
BsmI GAATGC 1 cut(s) 1211
BsnI GGCC 1 cut(s) 1060
Bsp1286I GDGCHC 4 cut(s) 110, 125, 1117, 1664
Bsp143I GATC 6 cut(s) 361, 643, 717, 1131, 1393, 1642
BspACI CCGC 5 cut(s) 531, 947, 1016, 1210, 1443
BspANI GGCC 1 cut(s) 1060
BspCNI CTCAG 4 cut(s) 445, 592, 907, 1117
BspLI GGNNCC 5 cut(s) 1170, 1260, 1298, 1447, 1661
BspPI GGATC 3 cut(s) 356, 725, 1139
BspT107I GGYRCC 1 cut(s) 1445
BsrFI RCCGGY 1 cut(s) 1605
BsrI ACTGG 2 cut(s) 47, 1143
BssAI RCCGGY 1 cut(s) 1605
BssECI CCNNGG 3 cut(s) 477, 546, 1292
BssMI GATC 6 cut(s) 361, 643, 717, 1131, 1393, 1642
BssNAI GTATAC 1 cut(s) 809
BssNI GRCGYC 1 cut(s) 631
BssT1I CCWWGG 1 cut(s) 477
Bst1107I GTATAC 1 cut(s) 809
Bst2UI CCWGG 3 cut(s) 132, 548, 1294
Bst4CI ACNGT 6 cut(s) 327, 1119, 1191, 1366, 1525, 1787
Bst6I CTCTTC 1 cut(s) 612
BstACI GRCGYC 1 cut(s) 631
BstDEI CTNAG 4 cut(s) 453, 579, 915, 1125
BstF5I GGATG 5 cut(s) 10, 403, 542, 1485, 1708
BstHHI GCGC 1 cut(s) 1077
BstKTI GATC 6 cut(s) 364, 646, 720, 1134, 1396, 1645
BstMBI GATC 6 cut(s) 361, 643, 717, 1131, 1393, 1642
BstMCI CGRYCG 1 cut(s) 1606
BstMWI GCNNNNNNNGC 3 cut(s) 515, 1081, 1454
BstNI CCWGG 3 cut(s) 132, 548, 1294
BstSCI CCNGG 3 cut(s) 130, 546, 1292
BstSFI CTRYAG 1 cut(s) 1365
BstSLI GKGCMC 1 cut(s) 1117
BstV1I GCAGC 3 cut(s) 562, 830, 956
BstV2I GAAGAC 3 cut(s) 84, 231, 626
BstXI CCANNNNNNTGG 1 cut(s) 1469
BstZ17I GTATAC 1 cut(s) 809
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 1 cut(s) 1060
BtsCI GGATG 5 cut(s) 10, 403, 542, 1485, 1708
CfoI GCGC 1 cut(s) 1077
Cfr10I RCCGGY 1 cut(s) 1605
Cfr13I GGNCC 2 cut(s) 134, 827
CseI GACGC 1 cut(s) 236
Csp6I GTAC 1 cut(s) 493
CspAI ACCGGT 1 cut(s) 1605
CviAII CATG 5 cut(s) 172, 215, 679, 1042, 1202
CviQI GTAC 1 cut(s) 493
DdeI CTNAG 4 cut(s) 453, 579, 915, 1125
DpnI GATC 6 cut(s) 363, 645, 719, 1133, 1395, 1644
DpnII GATC 6 cut(s) 361, 643, 717, 1131, 1393, 1642
Eam1104I CTCTTC 1 cut(s) 612
EarI CTCTTC 1 cut(s) 612
Ecl136II GAGCTC 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 477
Eco24I GRGCYC 2 cut(s) 125, 1664
Eco32I GATATC 1 cut(s) 1506
Eco47I GGWCC 2 cut(s) 134, 827
Eco53kI GAGCTC 1 cut(s) 123
Eco57I CTGAAG 4 cut(s) 72, 78, 267, 357
EcoICRI GAGCTC 1 cut(s) 123
EcoO109I RGGNCCY 2 cut(s) 134, 827
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 3 cut(s) 130, 546, 1292
EcoRV GATATC 1 cut(s) 1506
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 1036
EcoT38I GRGCYC 2 cut(s) 125, 1664
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 5 cut(s) 175, 218, 682, 1045, 1205
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 3 cut(s) 717, 787, 1082
FatI CATG 5 cut(s) 171, 214, 678, 1041, 1201
FbaI TGATCA 1 cut(s) 643
FblI GTMKAC 2 cut(s) 537, 808
Fnu4HI GCNGC 5 cut(s) 576, 819, 945, 948, 1210
FokI GGATG 5 cut(s) 17, 410, 529, 1492, 1715
FriOI GRGCYC 2 cut(s) 125, 1664
Fsp4HI GCNGC 5 cut(s) 576, 819, 945, 948, 1210
FspBI CTAG 4 cut(s) 627, 951, 1110, 1709
GlaI GCGC 1 cut(s) 1076
GluI GCNGC 5 cut(s) 576, 819, 945, 948, 1210
GsuI CTGGAG 2 cut(s) 114, 285
HaeIII GGCC 1 cut(s) 1060
HapII CCGG 1 cut(s) 1606
HgaI GACGC 1 cut(s) 236
HhaI GCGC 1 cut(s) 1077
Hin1I GRCGYC 1 cut(s) 631
Hin1II CATG 5 cut(s) 175, 218, 682, 1045, 1205
Hin6I GCGC 1 cut(s) 1075
HinP1I GCGC 1 cut(s) 1075
HincII GTYRAC 2 cut(s) 40, 1122
HindII GTYRAC 2 cut(s) 40, 1122
HpaII CCGG 1 cut(s) 1606
HphI GGTGA 4 cut(s) 230, 1169, 1525, 1565
Hpy166II GTNNAC 7 cut(s) 40, 495, 538, 799, 809, 1115, 1122
Hpy188I TCNGA 9 cut(s) 286, 361, 376, 409, 424, 454, 1136, 1146, 1356
Hpy188III TCNNGA 5 cut(s) 242, 269, 302, 395, 891
Hpy8I GTNNAC 7 cut(s) 40, 495, 538, 799, 809, 1115, 1122
Hpy99I CGWCG 1 cut(s) 1191
HpyAV CCTTC 3 cut(s) 147, 691, 1706
HpyCH4III ACNGT 6 cut(s) 327, 1119, 1191, 1366, 1525, 1787
HpyCH4IV ACGT 1 cut(s) 631
HpyCH4V TGCA 5 cut(s) 944, 1034, 1115, 1588, 1668
HpyF10VI GCNNNNNNNGC 3 cut(s) 515, 1081, 1454
HpyF3I CTNAG 4 cut(s) 453, 579, 915, 1125
HpySE526I ACGT 1 cut(s) 631
Hsp92I GRCGYC 1 cut(s) 631
Hsp92II CATG 5 cut(s) 175, 218, 682, 1045, 1205
HspAI GCGC 1 cut(s) 1075
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 6 cut(s) 361, 643, 717, 1131, 1393, 1642
LmnI GCTCC 7 cut(s) 120, 387, 425, 1258, 1543, 1612, 1659
Lsp1109I GCAGC 3 cut(s) 562, 830, 956
LweI GCATC 3 cut(s) 518, 1021, 1419
MaeI CTAG 4 cut(s) 627, 951, 1110, 1709
MaeII ACGT 1 cut(s) 631
MaeIII GTNAC 1 cut(s) 1335
MalI GATC 6 cut(s) 363, 645, 719, 1133, 1395, 1644
MboI GATC 6 cut(s) 361, 643, 717, 1131, 1393, 1642
MhlI GDGCHC 4 cut(s) 110, 125, 1117, 1664
MluCI AATT 6 cut(s) 76, 237, 274, 351, 500, 885
MlyI GAGTC 3 cut(s) 533, 769, 856
MmeI TCCRAC 5 cut(s) 470, 803, 1236, 1590, 1633
Mph1103I ATGCAT 1 cut(s) 1036
MroXI GAANNNNTTC 2 cut(s) 741, 1182
MseI TTAA 2 cut(s) 1764, 1789
MslI CAYNNNNRTG 2 cut(s) 1478, 1701
MspA1I CMGCKG 3 cut(s) 575, 947, 1084
MspI CCGG 1 cut(s) 1606
MspR9I CCNGG 3 cut(s) 132, 548, 1294
Mva1269I GAATGC 1 cut(s) 1211
MvaI CCWGG 3 cut(s) 132, 548, 1294
MwoI GCNNNNNNNGC 3 cut(s) 515, 1081, 1454
NdeII GATC 6 cut(s) 361, 643, 717, 1131, 1393, 1642
NlaIII CATG 5 cut(s) 175, 218, 682, 1045, 1205
NlaIV GGNNCC 5 cut(s) 1170, 1260, 1298, 1447, 1661
NmeAIII GCCGAG 1 cut(s) 1474
NmuCI GTSAC 1 cut(s) 1335
NsiI ATGCAT 1 cut(s) 1036
PctI GAATGC 1 cut(s) 1211
PdmI GAANNNNTTC 2 cut(s) 741, 1182
PfeI GAWTC 8 cut(s) 233, 293, 311, 356, 926, 1219, 1287, 1721
PflMI CCANNNNNTGG 1 cut(s) 764
PfoI TCCNGGA 1 cut(s) 130
PinAI ACCGGT 1 cut(s) 1605
PkrI GCNGC 5 cut(s) 577, 820, 946, 949, 1211
PleI GAGTC 3 cut(s) 533, 769, 856
PpsI GAGTC 3 cut(s) 533, 769, 856
PpuMI RGGWCCY 2 cut(s) 134, 827
Psp124BI GAGCTC 1 cut(s) 125
Psp5II RGGWCCY 2 cut(s) 134, 827
Psp6I CCWGG 3 cut(s) 130, 546, 1292
PspGI CCWGG 3 cut(s) 130, 546, 1292
PspN4I GGNNCC 5 cut(s) 1170, 1260, 1298, 1447, 1661
PspPI GGNCC 2 cut(s) 134, 827
PspPPI RGGWCCY 2 cut(s) 134, 827
PvuII CAGCTG 2 cut(s) 575, 1084
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
RseI CAYNNNNRTG 2 cut(s) 1478, 1701
SacI GAGCTC 1 cut(s) 125
SaqAI TTAA 2 cut(s) 1764, 1789
SatI GCNGC 5 cut(s) 576, 819, 945, 948, 1210
Sau3AI GATC 6 cut(s) 361, 643, 717, 1131, 1393, 1642
Sau96I GGNCC 2 cut(s) 134, 827
SchI GAGTC 3 cut(s) 533, 769, 856
ScrFI CCNGG 3 cut(s) 132, 548, 1294
SduI GDGCHC 4 cut(s) 110, 125, 1117, 1664
SfaNI GCATC 3 cut(s) 518, 1021, 1419
SfcI CTRYAG 1 cut(s) 1365
SinI GGWCC 2 cut(s) 134, 827
SmiMI CAYNNNNRTG 2 cut(s) 1478, 1701
Sse9I AATT 6 cut(s) 76, 237, 274, 351, 500, 885
SsiI CCGC 5 cut(s) 531, 947, 1016, 1210, 1443
SspMI CTAG 4 cut(s) 627, 951, 1110, 1709
SstI GAGCTC 1 cut(s) 125
StyD4I CCNGG 3 cut(s) 130, 546, 1292
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 6 cut(s) 327, 1119, 1191, 1366, 1525, 1787
TaiI ACGT 1 cut(s) 634
TaqI TCGA 3 cut(s) 504, 683, 1186
TasI AATT 6 cut(s) 76, 237, 274, 351, 500, 885
TauI GCSGC 2 cut(s) 950, 1212
TfiI GAWTC 8 cut(s) 233, 293, 311, 356, 926, 1219, 1287, 1721
Tru1I TTAA 2 cut(s) 1764, 1789
Tru9I TTAA 2 cut(s) 1764, 1789
TseFI GTSAC 1 cut(s) 1335
TseI GCWGC 3 cut(s) 575, 818, 944
Tsp45I GTSAC 1 cut(s) 1335
TspDTI ATGAA 8 cut(s) 430, 556, 734, 750, 861, 959, 1218, 1496
TspGWI ACGGA 2 cut(s) 1486, 1492
Van91I CCANNNNNTGG 1 cut(s) 764
VneI GTGCAC 1 cut(s) 1113
VpaK11BI GGWCC 2 cut(s) 134, 827
XapI RAATTY 3 cut(s) 76, 274, 500
XmiI GTMKAC 2 cut(s) 537, 808
XmnI GAANNNNTTC 2 cut(s) 741, 1182
XspI CTAG 4 cut(s) 627, 951, 1110, 1709
ZraI GACGTC 1 cut(s) 632
Zsp2I ATGCAT 1 cut(s) 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.