Rorug02G0333400

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000338
Physical Location & Seq
Forward (+)
41296799 .. 41298232
1434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0333400.1

Sequence Viewer

Length: 522 bp
ATGTACACAAGCTTCCTTCTCCTCTCTCTCCTCCTCTCACTCGCCGCCCTCTCCAACGCCCAAAACAGCGTCGTCACGATGGAGCTGTGGTGCGTGGCCAAGAACAACGCCGACGACTCTGCCCTCCAGTCCGCTCTCGACTGGGCCTGCAGTGCCGGCGGCGCCAACTGCAGCCCTATCCAGCAAGGTGGGCCCTGCTACGACCCTTCTGACATCCAGAACACCGCGTCGTACGCCTTCAACGACTACTTTCTCAAACACGGCATGACCGATGACAGCTGCAACTTCGATAACACTGCCGCAGTCACCTCTTTGAATCCCAGTTTTGGCAATTGCAAGTTCCCATCCAGCTTGTCAGGGAGCAATAGAAGTTCGAGTTCATCGACAGCATCATATGGGTTGGGGCCTAGTCAAGATCTGAATGGCAGCAATAAAATTTCGCAGCAACTGGTTTTGCTGCCCTTGATTACCAGTCTTTTGCTCATTGCCTCCTATAATACATGGGTCATCTGTTTATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

173

Amino Acids

18.28

Weight (kDa)

4.19

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 29 - 100 1.6e-20 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 134
AccII CGCG 1 cut(s) 227
AciI CCGC 5 cut(s) 45, 132, 159, 225, 300
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 2 cut(s) 5, 233
AfiI CCNNNNNNNGG 1 cut(s) 326
AgsI TTSAA 2 cut(s) 241, 316
AluBI AGCT 4 cut(s) 12, 85, 279, 351
AluI AGCT 4 cut(s) 12, 85, 279, 351
AlwNI CAGNNNCTG 1 cut(s) 448
AoxI GGCC 4 cut(s) 96, 144, 191, 404
ApaI GGGCCC 1 cut(s) 195
ApeKI GCWGC 5 cut(s) 171, 279, 426, 442, 457
ApoI RAATTY 1 cut(s) 435
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 4 cut(s) 144, 191, 192, 404
AsuHPI GGTGA 1 cut(s) 298
BaeGI GKGCMC 1 cut(s) 195
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 195
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbvI GCAGC 5 cut(s) 183, 266, 438, 444, 454
BccI CCATC 2 cut(s) 73, 352
BceAI ACGGC 1 cut(s) 277
BcgI CGANNNNNNTGC 4 cut(s) 101, 135, 278, 312
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 2 cut(s) 148, 169
BfoI RGCGCY 1 cut(s) 165
BglII AGATCT 1 cut(s) 415
BisI GCNGC 8 cut(s) 45, 160, 172, 280, 300, 427, 443, 458
BlsI GCNGC 8 cut(s) 46, 161, 173, 281, 301, 428, 444, 459
BmgT120I GGNCC 4 cut(s) 144, 191, 192, 404
BmiI GGNNCC 3 cut(s) 163, 193, 405
BmrI ACTGGG 2 cut(s) 151, 315
BmsI GCATC 1 cut(s) 398
BmuI ACTGGG 2 cut(s) 151, 315
BpmI CTGGAG 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 5 cut(s) 127, 146, 321, 453, 471
Bse3DI GCAATG 1 cut(s) 483
BseGI GGATG 2 cut(s) 213, 344
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMI GCAATG 1 cut(s) 483
BseNI ACTGG 5 cut(s) 127, 146, 321, 453, 471
BseRI GAGGAG 3 cut(s) 11, 20, 23
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 5 cut(s) 183, 266, 438, 444, 454
Bsh1236I CGCG 1 cut(s) 227
BshFI GGCC 4 cut(s) 98, 146, 193, 406
BshNI GGYRCC 1 cut(s) 161
BsiSI CCGG 1 cut(s) 156
BsiWI CGTACG 1 cut(s) 231
BslI CCNNNNNNNGG 1 cut(s) 326
BsnI GGCC 4 cut(s) 98, 146, 193, 406
Bsp120I GGGCCC 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 415
BspACI CCGC 5 cut(s) 45, 132, 159, 225, 300
BspANI GGCC 4 cut(s) 98, 146, 193, 406
BspFNI CGCG 1 cut(s) 227
BspLI GGNNCC 3 cut(s) 163, 193, 405
BspMAI CTGCAG 2 cut(s) 152, 173
BspT107I GGYRCC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 5 cut(s) 127, 146, 321, 453, 471
BssAI RCCGGY 1 cut(s) 155
BssMI GATC 1 cut(s) 415
BssNI GRCGYC 1 cut(s) 162
BstACI GRCGYC 1 cut(s) 162
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 148, 157
BstF5I GGATG 2 cut(s) 213, 344
BstFNI CGCG 1 cut(s) 227
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 418
BstMBI GATC 1 cut(s) 415
BstMWI GCNNNNNNNGC 6 cut(s) 152, 156, 161, 168, 190, 233
BstSFI CTRYAG 2 cut(s) 148, 169
BstSLI GKGCMC 1 cut(s) 195
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 5 cut(s) 183, 266, 438, 444, 454
BstX2I RGATCY 1 cut(s) 415
BstXI CCANNNNNNTGG 1 cut(s) 188
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 4 cut(s) 98, 146, 193, 406
BtsCI GGATG 2 cut(s) 213, 344
BtsI GCAGTG 2 cut(s) 157, 294
BtsIMutI CAGTG 2 cut(s) 157, 294
Cac8I GCNNGC 2 cut(s) 148, 157
CaiI CAGNNNCTG 1 cut(s) 448
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 4 cut(s) 144, 191, 192, 404
CseI GACGC 2 cut(s) 58, 216
Csp6I GTAC 2 cut(s) 4, 232
CviAII CATG 2 cut(s) 265, 501
CviJI RGCY 9 cut(s) 12, 85, 98, 146, 174, 193, 279, 351, 406
CviKI_1 RGCY 9 cut(s) 12, 85, 98, 146, 174, 193, 279, 351, 406
CviQI GTAC 2 cut(s) 4, 232
DinI GGCGCC 1 cut(s) 163
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
EaeI YGGCCR 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 195
EcoO109I RGGNCCY 2 cut(s) 192, 404
EcoT38I GRGCYC 1 cut(s) 195
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
FaeI CATG 2 cut(s) 268, 504
FaiI YATR 5 cut(s) 266, 394, 396, 495, 502
FatI CATG 2 cut(s) 264, 500
FauNDI CATATG 1 cut(s) 394
Fnu4HI GCNGC 8 cut(s) 45, 160, 172, 280, 300, 427, 443, 458
FokI GGATG 2 cut(s) 200, 331
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 8 cut(s) 45, 160, 172, 280, 300, 427, 443, 458
FspBI CTAG 1 cut(s) 408
GlaI GCGC 1 cut(s) 163
GluI GCNGC 8 cut(s) 45, 160, 172, 280, 300, 427, 443, 458
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 4 cut(s) 98, 146, 193, 406
HapII CCGG 1 cut(s) 156
HgaI GACGC 2 cut(s) 58, 216
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 2 cut(s) 268, 504
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 2 cut(s) 116, 316
HpaII CCGG 1 cut(s) 156
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 211, 420
Hpy188III TCNNGA 5 cut(s) 76, 137, 217, 413, 519
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 3 cut(s) 74, 116, 232
HpyAV CCTTC 3 cut(s) 26, 216, 247
HpyCH4V TGCA 4 cut(s) 150, 171, 282, 336
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 156, 161, 168, 190, 233
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 2 cut(s) 268, 504
HspAI GCGC 1 cut(s) 162
KasI GGCGCC 1 cut(s) 161
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Kzo9I GATC 1 cut(s) 415
LmnI GCTCC 2 cut(s) 82, 360
Lsp1109I GCAGC 5 cut(s) 183, 266, 438, 444, 454
LweI GCATC 1 cut(s) 398
MaeI CTAG 1 cut(s) 408
MaeIII GTNAC 2 cut(s) 73, 304
MalI GATC 1 cut(s) 417
MbiI CCGCTC 1 cut(s) 134
MboI GATC 1 cut(s) 415
MfeI CAATTG 1 cut(s) 331
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 195
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 2 cut(s) 331, 435
MluNI TGGCCA 1 cut(s) 98
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 7 cut(s) 32, 41, 44, 59, 134, 319, 499
Mox20I TGGCCA 1 cut(s) 98
MroNI GCCGGC 1 cut(s) 155
MscI TGGCCA 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 279
MspI CCGG 1 cut(s) 156
MunI CAATTG 1 cut(s) 331
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 6 cut(s) 152, 156, 161, 168, 190, 233
NaeI GCCGGC 1 cut(s) 157
NarI GGCGCC 1 cut(s) 162
NdeI CATATG 1 cut(s) 394
NdeII GATC 1 cut(s) 415
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 2 cut(s) 268, 504
NlaIV GGNNCC 3 cut(s) 163, 193, 405
NmuCI GTSAC 2 cut(s) 73, 304
PcsI WCGNNNNNNNCGW 3 cut(s) 240, 267, 380
PdiI GCCGGC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 316
Pfl23II CGTACG 1 cut(s) 231
PkrI GCNGC 8 cut(s) 46, 161, 173, 281, 301, 428, 444, 459
PleI GAGTC 1 cut(s) 110
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 110
PspLI CGTACG 1 cut(s) 231
PspN4I GGNNCC 3 cut(s) 163, 193, 405
PspOMI GGGCCC 1 cut(s) 191
PspPI GGNCC 4 cut(s) 144, 191, 192, 404
PstI CTGCAG 2 cut(s) 152, 173
PstNI CAGNNNCTG 1 cut(s) 448
PsuI RGATCY 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 279
RsaI GTAC 2 cut(s) 5, 233
RsaNI GTAC 2 cut(s) 4, 232
SatI GCNGC 8 cut(s) 45, 160, 172, 280, 300, 427, 443, 458
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 4 cut(s) 144, 191, 192, 404
SchI GAGTC 1 cut(s) 110
SduI GDGCHC 1 cut(s) 195
SetI ASST 6 cut(s) 14, 87, 190, 281, 311, 353
SfaNI GCATC 1 cut(s) 398
SfcI CTRYAG 2 cut(s) 148, 169
SfoI GGCGCC 1 cut(s) 163
Sse9I AATT 2 cut(s) 331, 435
SsiI CCGC 5 cut(s) 45, 132, 159, 225, 300
SspDI GGCGCC 1 cut(s) 161
SspMI CTAG 1 cut(s) 408
TaqI TCGA 4 cut(s) 138, 288, 374, 383
TaqII GACCGA 1 cut(s) 284
TasI AATT 2 cut(s) 331, 435
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 47, 162, 302
TfiI GAWTC 1 cut(s) 316
TscAI CASTG 2 cut(s) 157, 301
TseFI GTSAC 2 cut(s) 73, 304
TseI GCWGC 5 cut(s) 171, 279, 426, 442, 457
Tsp45I GTSAC 2 cut(s) 73, 304
TspDTI ATGAA 1 cut(s) 369
TspRI CASTG 2 cut(s) 157, 301
XapI RAATTY 1 cut(s) 435
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.