Rorug06G0043800

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000338
Physical Location & Seq
Forward (+)
5587648 .. 5589176
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0043800.1

Sequence Viewer

Length: 1050 bp
ATGAATATTGAGCTTGACAAACTCAGTAACTTACCAAGGGATGTTACGGAAAAGATTTTGTCAATTTTGCCAATAAAGGAGGCAGTGAGGACAAGTATATTATCGAGCGAGTGGAGGTACAAATGGGCTATGCTTCCAGATCTTGTGTTTGATGAACTGGGTTTCCCAGGAGTACTTTATAAGCTCGACAAATTAGTGAAAATGGTTGATCATGTGCTCTTCCTTCACATTGGTCCAATAAACAAGGTCAAGCTTTCTCATAAAGGTCTTAAGGGCACCGCAGATATTGATAGATGGATTCTTCATCTATCAAGAAATTCTATGAAAGAGTTCATATTTAAGTTTTGGAAAAAGCCTGAATACAAGATGCCTTCATATTGTCATGGTTTCACCAAGCTCAAGATTGATGCACCAAATCTCCGAATCTTTTGCCTTCTAGGTGCTTTTGAGGATCTTAGTTTTCAGAACACCTTAAAGCTTACAAGTATTGCCATTTATTTAACAGCTAATGGTGACCAAAGAAGACAGAAACCTGGCATTTCTAGCAATTTGCTCAAGCTTTTTGATCAGTGTGCTTATGTTCAAACTGCTAATATTCTTGGTTACTTTTTAAAGTATTTAGCTATTGGTGACTTGCCGGTAAAGCGTCCTAAACCATGTCTGCATCTGAAGTTGCTTCGTATAAACATAGATTTCAATGATCTGAAGGAGGTTTTATCTACTTTATACCTTCTGAGAAGCGCCCCTGCTCTACAAAAATTATATATCAGAGTTCGTCAAGAGGGACAGGCTCTTGTGGGAGAGGTGAACTCTTGGTTAGATGGCAACCAGAATTTCCAACTCGGAAAACTGCAAATTGTGGAAGTACGTGGCATCACTAGCACCAAAGCTGTACTAGATTTCATCAAATTTCTGCTTTTAAATTCACCGATGCTCGAGAAGGTGTTTGTTAAGCCTGCATCTCACTATAGTTCTTCGGAACTATTAGTAAGGTTGAGCCAGTTTAGGCGCTCCTCAGCACTTGTGGAGATAATGCACTTGGACCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

349

Amino Acids

40.09

Weight (kDa)

9.58

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 8 - 45 1.6e-06 F-box domain
LRR_At1g61320_AtMIF1 PF23622 127 - 318 9.1e-08 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 180
AccB1I GGYRCC 1 cut(s) 275
AciI CCGC 1 cut(s) 279
AclWI GGATC 1 cut(s) 459
AcsI RAATTY 4 cut(s) 316, 832, 908, 922
AcuI CTGAAG 2 cut(s) 689, 725
AfaI GTAC 4 cut(s) 119, 174, 867, 894
AflII CTTAAG 1 cut(s) 269
AgsI TTSAA 2 cut(s) 584, 697
AjnI CCWGG 2 cut(s) 166, 532
AloI GAACNNNNNNTCC 2 cut(s) 147, 179
AluBI AGCT 9 cut(s) 13, 184, 253, 397, 478, 506, 559, 623, 890
AluI AGCT 9 cut(s) 13, 184, 253, 397, 478, 506, 559, 623, 890
Alw21I GWGCWC 1 cut(s) 219
AlwI GGATC 1 cut(s) 459
Ama87I CYCGRG 1 cut(s) 935
ApoI RAATTY 4 cut(s) 316, 832, 908, 922
Asp700I GAANNNNTTC 1 cut(s) 329
AspLEI GCGC 2 cut(s) 743, 1011
AspS9I GGNCC 2 cut(s) 233, 1042
AsuHPI GGTGA 5 cut(s) 382, 524, 641, 817, 918
AvaI CYCGRG 1 cut(s) 935
AvaII GGWCC 2 cut(s) 233, 1042
BaeGI GKGCMC 1 cut(s) 278
BanI GGYRCC 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 529
Bbv12I GWGCWC 1 cut(s) 219
BbvCI CCTCAGC 1 cut(s) 1015
BccI CCATC 2 cut(s) 288, 815
BcgI CGANNNNNNTGC 2 cut(s) 411, 445
BciT130I CCWGG 2 cut(s) 168, 534
BclI TGATCA 2 cut(s) 208, 565
BfaI CTAG 4 cut(s) 437, 543, 879, 896
BfmI CTRYAG 1 cut(s) 967
BfoI RGCGCY 2 cut(s) 744, 1012
BfrI CTTAAG 1 cut(s) 269
BglII AGATCT 1 cut(s) 139
BmcAI AGTACT 1 cut(s) 174
Bme1390I CCNGG 2 cut(s) 168, 534
Bme18I GGWCC 2 cut(s) 233, 1042
BmeT110I CYCGRG 1 cut(s) 935
BmgT120I GGNCC 2 cut(s) 233, 1042
BmiI GGNNCC 2 cut(s) 277, 1044
BmrFI CCNGG 2 cut(s) 168, 534
BmrI ACTGGG 1 cut(s) 167
BmsI GCATC 6 cut(s) 357, 397, 673, 882, 921, 968
BmuI ACTGGG 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 529
BplI GAGNNNNNCTC 2 cut(s) 794, 826
Bpu10I CCTNAGC 1 cut(s) 1015
BpuEI CTTGAG 2 cut(s) 383, 539
BsaAI YACGTR 1 cut(s) 869
BsaJI CCNNGG 2 cut(s) 35, 166
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bse118I RCCGGY 1 cut(s) 637
Bse1I ACTGG 2 cut(s) 162, 1000
BseBI CCWGG 2 cut(s) 168, 534
BseDI CCNNGG 2 cut(s) 35, 166
BseGI GGATG 1 cut(s) 46
BseMII CTCAG 3 cut(s) 37, 725, 1029
BseNI ACTGG 2 cut(s) 162, 1000
BseRI GAGGAG 1 cut(s) 1003
BseSI GKGCMC 1 cut(s) 278
BshNI GGYRCC 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 219
BsiHKCI CYCGRG 1 cut(s) 935
BsiSI CCGG 1 cut(s) 638
BslFI GGGAC 1 cut(s) 798
BsmFI GGGAC 1 cut(s) 798
BsoBI CYCGRG 1 cut(s) 935
Bsp1286I GDGCHC 2 cut(s) 219, 278
Bsp143I GATC 5 cut(s) 139, 208, 451, 565, 700
BspACI CCGC 1 cut(s) 279
BspCNI CTCAG 3 cut(s) 36, 726, 1028
BspLI GGNNCC 2 cut(s) 277, 1044
BspPI GGATC 1 cut(s) 459
BspQI GCTCTTC 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 275
BspTI CTTAAG 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 637
BsrI ACTGG 2 cut(s) 162, 1000
BssAI RCCGGY 1 cut(s) 637
BssECI CCNNGG 2 cut(s) 35, 166
BssMI GATC 5 cut(s) 139, 208, 451, 565, 700
BssT1I CCWWGG 1 cut(s) 35
Bst2UI CCWGG 2 cut(s) 168, 534
Bst6I CTCTTC 1 cut(s) 224
BstAFI CTTAAG 1 cut(s) 269
BstBAI YACGTR 1 cut(s) 869
BstC8I GCNNGC 1 cut(s) 957
BstDEI CTNAG 4 cut(s) 23, 455, 734, 1015
BstEII GGTNACC 1 cut(s) 512
BstF5I GGATG 1 cut(s) 46
BstH2I RGCGCY 2 cut(s) 744, 1012
BstHHI GCGC 2 cut(s) 743, 1011
BstKTI GATC 5 cut(s) 142, 211, 454, 568, 703
BstMBI GATC 5 cut(s) 139, 208, 451, 565, 700
BstMWI GCNNNNNNNGC 3 cut(s) 543, 643, 879
BstNI CCWGG 2 cut(s) 168, 534
BstPI GGTNACC 1 cut(s) 512
BstSCI CCNGG 2 cut(s) 166, 532
BstSFI CTRYAG 1 cut(s) 967
BstSLI GKGCMC 1 cut(s) 278
BstV2I GAAGAC 1 cut(s) 529
BstX2I RGATCY 2 cut(s) 139, 451
BstYI RGATCY 2 cut(s) 139, 451
BtsCI GGATG 1 cut(s) 46
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 2 cut(s) 90, 575
Cac8I GCNNGC 1 cut(s) 957
CfoI GCGC 2 cut(s) 743, 1011
Cfr10I RCCGGY 1 cut(s) 637
Cfr13I GGNCC 2 cut(s) 233, 1042
CseI GACGC 1 cut(s) 635
Csp6I GTAC 4 cut(s) 118, 173, 866, 893
CviAII CATG 4 cut(s) 212, 383, 657, 1047
CviQI GTAC 4 cut(s) 118, 173, 866, 893
DdeI CTNAG 4 cut(s) 23, 455, 734, 1015
DpnI GATC 5 cut(s) 141, 210, 453, 567, 702
DpnII GATC 5 cut(s) 139, 208, 451, 565, 700
DraI TTTAAA 2 cut(s) 612, 921
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Eco130I CCWWGG 1 cut(s) 35
Eco47I GGWCC 2 cut(s) 233, 1042
Eco57I CTGAAG 2 cut(s) 689, 725
Eco88I CYCGRG 1 cut(s) 935
Eco91I GGTNACC 1 cut(s) 512
EcoO65I GGTNACC 1 cut(s) 512
EcoRII CCWGG 2 cut(s) 166, 532
EcoT14I CCWWGG 1 cut(s) 35
ErhI CCWWGG 1 cut(s) 35
FaeI CATG 4 cut(s) 215, 386, 660, 1050
FaqI GGGAC 1 cut(s) 798
FatI CATG 4 cut(s) 211, 382, 656, 1046
FbaI TGATCA 2 cut(s) 208, 565
FokI GGATG 1 cut(s) 53
FspBI CTAG 4 cut(s) 437, 543, 879, 896
GlaI GCGC 2 cut(s) 742, 1010
HaeII RGCGCY 2 cut(s) 744, 1012
HapII CCGG 1 cut(s) 638
HgaI GACGC 1 cut(s) 635
HhaI GCGC 2 cut(s) 743, 1011
Hin1II CATG 4 cut(s) 215, 386, 660, 1050
Hin6I GCGC 2 cut(s) 741, 1009
HinP1I GCGC 2 cut(s) 741, 1009
HindIII AAGCTT 3 cut(s) 251, 476, 557
HinfI GANTC 2 cut(s) 298, 423
HpaII CCGG 1 cut(s) 638
HphI GGTGA 5 cut(s) 382, 524, 641, 817, 918
Hpy166II GTNNAC 1 cut(s) 808
Hpy188I TCNGA 8 cut(s) 422, 465, 669, 705, 735, 770, 845, 979
Hpy188III TCNNGA 5 cut(s) 137, 312, 400, 779, 937
Hpy8I GTNNAC 1 cut(s) 808
HpyAV CCTTC 6 cut(s) 233, 381, 443, 700, 740, 934
HpyCH4IV ACGT 1 cut(s) 868
HpyCH4V TGCA 5 cut(s) 410, 664, 853, 959, 1036
HpyF10VI GCNNNNNNNGC 3 cut(s) 543, 643, 879
HpyF3I CTNAG 4 cut(s) 23, 455, 734, 1015
HpySE526I ACGT 1 cut(s) 868
Hsp92II CATG 4 cut(s) 215, 386, 660, 1050
HspAI GCGC 2 cut(s) 741, 1009
Ksp22I TGATCA 2 cut(s) 208, 565
Kzo9I GATC 5 cut(s) 139, 208, 451, 565, 700
LguI GCTCTTC 1 cut(s) 224
LmnI GCTCC 1 cut(s) 1016
LweI GCATC 6 cut(s) 357, 397, 673, 882, 921, 968
MaeI CTAG 4 cut(s) 437, 543, 879, 896
MaeII ACGT 1 cut(s) 868
MaeIII GTNAC 5 cut(s) 26, 43, 512, 602, 629
MalI GATC 5 cut(s) 141, 210, 453, 567, 702
MboI GATC 5 cut(s) 139, 208, 451, 565, 700
MboII GAAGA 4 cut(s) 211, 293, 534, 966
MflI RGATCY 2 cut(s) 139, 451
MhlI GDGCHC 2 cut(s) 219, 278
MluCI AATT 9 cut(s) 63, 191, 316, 547, 758, 832, 855, 908, 922
MmeI TCCRAC 1 cut(s) 862
MnlI CCTC 8 cut(s) 73, 81, 108, 442, 703, 775, 796, 1024
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 7 cut(s) 270, 339, 473, 500, 611, 920, 951
MspCI CTTAAG 1 cut(s) 269
MspI CCGG 1 cut(s) 638
MspR9I CCNGG 2 cut(s) 168, 534
MvaI CCWGG 2 cut(s) 168, 534
MwoI GCNNNNNNNGC 3 cut(s) 543, 643, 879
NdeII GATC 5 cut(s) 139, 208, 451, 565, 700
NlaIII CATG 4 cut(s) 215, 386, 660, 1050
NlaIV GGNNCC 2 cut(s) 277, 1044
NmuCI GTSAC 2 cut(s) 512, 629
PaeR7I CTCGAG 1 cut(s) 935
PciSI GCTCTTC 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 2 cut(s) 298, 423
Ppu21I YACGTR 1 cut(s) 869
PsiI TTATAA 1 cut(s) 180
Psp6I CCWGG 2 cut(s) 166, 532
PspEI GGTNACC 1 cut(s) 512
PspGI CCWGG 2 cut(s) 166, 532
PspN4I GGNNCC 2 cut(s) 277, 1044
PspPI GGNCC 2 cut(s) 233, 1042
PsuI RGATCY 2 cut(s) 139, 451
RsaI GTAC 4 cut(s) 119, 174, 867, 894
RsaNI GTAC 4 cut(s) 118, 173, 866, 893
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 7 cut(s) 270, 339, 473, 500, 611, 920, 951
Sau3AI GATC 5 cut(s) 139, 208, 451, 565, 700
Sau96I GGNCC 2 cut(s) 233, 1042
ScaI AGTACT 1 cut(s) 174
ScrFI CCNGG 2 cut(s) 168, 534
SduI GDGCHC 2 cut(s) 219, 278
SfaNI GCATC 6 cut(s) 357, 397, 673, 882, 921, 968
SfcI CTRYAG 1 cut(s) 967
Sfr274I CTCGAG 1 cut(s) 935
SinI GGWCC 2 cut(s) 233, 1042
SlaI CTCGAG 1 cut(s) 935
SmlI CTYRAG 4 cut(s) 269, 398, 554, 935
SmoI CTYRAG 4 cut(s) 269, 398, 554, 935
Sse9I AATT 9 cut(s) 63, 191, 316, 547, 758, 832, 855, 908, 922
SsiI CCGC 1 cut(s) 279
SspI AATATT 2 cut(s) 7, 595
SspMI CTAG 4 cut(s) 437, 543, 879, 896
StyD4I CCNGG 2 cut(s) 166, 532
StyI CCWWGG 1 cut(s) 35
TaiI ACGT 1 cut(s) 871
TaqI TCGA 3 cut(s) 104, 186, 936
TasI AATT 9 cut(s) 63, 191, 316, 547, 758, 832, 855, 908, 922
TatI WGTACW 2 cut(s) 172, 892
TfiI GAWTC 2 cut(s) 298, 423
Tru1I TTAA 7 cut(s) 270, 339, 473, 500, 611, 920, 951
Tru9I TTAA 7 cut(s) 270, 339, 473, 500, 611, 920, 951
TscAI CASTG 2 cut(s) 90, 575
TseFI GTSAC 2 cut(s) 512, 629
Tsp45I GTSAC 2 cut(s) 512, 629
TspDTI ATGAA 7 cut(s) 17, 168, 293, 322, 338, 363, 892
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 2 cut(s) 90, 575
Vha464I CTTAAG 1 cut(s) 269
VpaK11BI GGWCC 2 cut(s) 233, 1042
XapI RAATTY 4 cut(s) 316, 832, 908, 922
XhoI CTCGAG 1 cut(s) 935
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 4 cut(s) 437, 543, 879, 896
ZrmI AGTACT 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.