Rh1AG053300

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000338
Physical Location & Seq
Reverse (-)
8889254 .. 8889958
705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG053300.1

Sequence Viewer

Length: 705 bp
ATGGGTTTGGGACTCCAGCTGATCTTGGTCAAATCCACCGCTTATTTTTACTATGAGAATGTGGCATTGACTCCTAAAGGAGTTTGGAGTACAATATCCCGCTTTTTGTATGATGTGGAGCCTGAGTTTATTGATTCGAAGTATTTTTGTGCTGCTGCAAGAAAAAGGGGCTATGTTCACAATCTTCCAATCAAAAAAAGATTTTCTCTCGTTCCCCTTCCCCCACAAACTACATTTGATGCATTTCCCATGACAAGGAGGTGGTGGCCTACTTGGGATGATCAGACAAAGCTAAAGTGCTTACAGACTTGCATTGCTAGTGCAACAATTACTGAGAGGATCATGAAGGCTCTTAAAGACTATGACAAAGATGAAACTCTACCTGAAAGTGTCCGTCGCTATGTTCTTTATGAATGCCGCAAGTGGAATCTTGTTTGGGTAGGAAAGAAAAAGGTGGCCCCACTTGAGCCCGATGAAGTAGAAATGCTTTTGGGTTTCTCTAGAGACCACACAAGGGGAATAAGCAGGAAAGATAGACACAAGTCACTGGGAAATTCATTCCAGGAAAGTTCTGTCTATTTGCATTTGCAATTTTGCATTGAACATTTCTTTCTTCCTTATTGTTCTGAATATTTCAGACAGTTCCATTTGCTTTGGTTTTTTGTTCTGTTCACAAGCTTGATCTGTATTTCAACTTCTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

234

Amino Acids

27.69

Weight (kDa)

9.09

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 39, 100, 418
AclWI GGATC 1 cut(s) 347
AcsI RAATTY 1 cut(s) 553
AfaI GTAC 1 cut(s) 91
AfiI CCNNNNNNNGG 2 cut(s) 255, 514
AgsI TTSAA 2 cut(s) 602, 693
AjnI CCWGG 1 cut(s) 561
AluBI AGCT 3 cut(s) 19, 292, 678
AluI AGCT 3 cut(s) 19, 292, 678
Alw26I GTCTC 1 cut(s) 498
AlwI GGATC 1 cut(s) 347
AoxI GGCC 2 cut(s) 266, 456
ApeKI GCWGC 2 cut(s) 152, 155
ApoI RAATTY 1 cut(s) 553
AspS9I GGNCC 1 cut(s) 457
AsuII TTCGAA 1 cut(s) 137
BanII GRGCYC 1 cut(s) 471
BarI GAAGNNNNNNTAC 1 cut(s) 679
BbvI GCAGC 2 cut(s) 139, 142
BciT130I CCWGG 1 cut(s) 563
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 498
BfaI CTAG 2 cut(s) 318, 501
BisI GCNGC 3 cut(s) 153, 156, 418
BlsI GCNGC 3 cut(s) 154, 157, 419
Bme1390I CCNGG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 457
BmiI GGNNCC 2 cut(s) 120, 459
BmrFI CCNGG 1 cut(s) 563
BmrI ACTGGG 1 cut(s) 557
BmsI GCATC 1 cut(s) 229
BmuI ACTGGG 1 cut(s) 557
BoxI GACNNNNGTC 1 cut(s) 541
Bpu14I TTCGAA 1 cut(s) 137
BpuEI CTTGAG 1 cut(s) 485
BsaI GGTCTC 1 cut(s) 498
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 2 cut(s) 255, 514
Bse1I ACTGG 1 cut(s) 552
Bse3DI GCAATG 1 cut(s) 312
BseBI CCWGG 1 cut(s) 563
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 2 cut(s) 255, 514
BseMI GCAATG 1 cut(s) 312
BseMII CTCAG 2 cut(s) 114, 324
BseNI ACTGG 1 cut(s) 552
BseXI GCAGC 2 cut(s) 139, 142
BshFI GGCC 2 cut(s) 268, 458
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 2 cut(s) 255, 514
BsmAI GTCTC 1 cut(s) 498
BsmFI GGGAC 1 cut(s) 24
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 2 cut(s) 268, 458
Bso31I GGTCTC 1 cut(s) 498
Bsp119I TTCGAA 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 4 cut(s) 21, 280, 339, 681
BspACI CCGC 3 cut(s) 39, 100, 418
BspANI GGCC 2 cut(s) 268, 458
BspCNI CTCAG 2 cut(s) 115, 325
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 2 cut(s) 120, 459
BspPI GGATC 1 cut(s) 347
BspT104I TTCGAA 1 cut(s) 137
BspTNI GGTCTC 1 cut(s) 498
BsrDI GCAATG 1 cut(s) 312
BsrI ACTGG 1 cut(s) 552
BssMI GATC 4 cut(s) 21, 280, 339, 681
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 1 cut(s) 642
BstBI TTCGAA 1 cut(s) 137
BstDEI CTNAG 2 cut(s) 123, 333
BstF5I GGATG 1 cut(s) 283
BstKTI GATC 4 cut(s) 24, 283, 342, 684
BstMAI GTCTC 1 cut(s) 498
BstMBI GATC 4 cut(s) 21, 280, 339, 681
BstNI CCWGG 1 cut(s) 563
BstPAI GACNNNNGTC 1 cut(s) 541
BstSCI CCNGG 1 cut(s) 561
BstV1I GCAGC 2 cut(s) 139, 142
BsuRI GGCC 2 cut(s) 268, 458
BtsCI GGATG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 545
CciI TCATGA 1 cut(s) 342
Cfr13I GGNCC 1 cut(s) 457
Csp6I GTAC 1 cut(s) 90
CviAII CATG 2 cut(s) 250, 343
CviJI RGCY 9 cut(s) 19, 121, 171, 268, 292, 350, 458, 469, 678
CviKI_1 RGCY 9 cut(s) 19, 121, 171, 268, 292, 350, 458, 469, 678
CviQI GTAC 1 cut(s) 90
DdeI CTNAG 2 cut(s) 123, 333
DpnI GATC 4 cut(s) 23, 282, 341, 683
DpnII GATC 4 cut(s) 21, 280, 339, 681
Eco24I GRGCYC 1 cut(s) 471
Eco31I GGTCTC 1 cut(s) 498
EcoRII CCWGG 1 cut(s) 561
EcoT22I ATGCAT 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 471
FaeI CATG 2 cut(s) 253, 346
FaiI YATR 9 cut(s) 54, 111, 174, 251, 344, 363, 402, 411, 703
FaqI GGGAC 1 cut(s) 24
FatI CATG 2 cut(s) 249, 342
FauI CCCGC 1 cut(s) 107
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 3 cut(s) 153, 156, 418
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 3 cut(s) 153, 156, 418
FspBI CTAG 2 cut(s) 318, 501
GluI GCNGC 3 cut(s) 153, 156, 418
HaeIII GGCC 2 cut(s) 268, 458
Hin1II CATG 2 cut(s) 253, 346
HindIII AAGCTT 1 cut(s) 676
HinfI GANTC 4 cut(s) 12, 70, 134, 427
Hpy166II GTNNAC 2 cut(s) 178, 672
Hpy188I TCNGA 3 cut(s) 285, 628, 638
Hpy188III TCNNGA 2 cut(s) 343, 501
Hpy8I GTNNAC 2 cut(s) 178, 672
Hpy99I CGWCG 1 cut(s) 399
HpyAV CCTTC 2 cut(s) 227, 340
HpyCH4III ACNGT 1 cut(s) 642
HpyCH4V TGCA 7 cut(s) 158, 242, 312, 323, 583, 589, 597
HpyF3I CTNAG 2 cut(s) 123, 333
Hsp92II CATG 2 cut(s) 253, 346
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 4 cut(s) 21, 280, 339, 681
LmnI GCTCC 1 cut(s) 118
LpnPI CCDG 7 cut(s) 29, 135, 396, 511, 533, 548, 575
Lsp1109I GCAGC 2 cut(s) 139, 142
LweI GCATC 1 cut(s) 229
MaeI CTAG 2 cut(s) 318, 501
MaeIII GTNAC 1 cut(s) 543
MalI GATC 4 cut(s) 23, 282, 341, 683
MboI GATC 4 cut(s) 21, 280, 339, 681
MboII GAAGA 2 cut(s) 176, 605
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 3 cut(s) 327, 553, 590
MlyI GAGTC 2 cut(s) 6, 64
MnlI CCTC 2 cut(s) 252, 330
Mph1103I ATGCAT 1 cut(s) 244
MseI TTAA 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 19
MspR9I CCNGG 1 cut(s) 563
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 1 cut(s) 563
NdeII GATC 4 cut(s) 21, 280, 339, 681
NlaIII CATG 2 cut(s) 253, 346
NlaIV GGNNCC 2 cut(s) 120, 459
NmuCI GTSAC 1 cut(s) 543
NsiI ATGCAT 1 cut(s) 244
NspV TTCGAA 1 cut(s) 137
PagI TCATGA 1 cut(s) 342
PctI GAATGC 1 cut(s) 419
PfeI GAWTC 2 cut(s) 134, 427
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 3 cut(s) 154, 157, 419
PleI GAGTC 2 cut(s) 6, 64
PpsI GAGTC 2 cut(s) 6, 64
PshAI GACNNNNGTC 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 561
PspGI CCWGG 1 cut(s) 561
PspN4I GGNNCC 2 cut(s) 120, 459
PspPI GGNCC 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 19
RsaI GTAC 1 cut(s) 91
RsaNI GTAC 1 cut(s) 90
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 3 cut(s) 153, 156, 418
Sau3AI GATC 4 cut(s) 21, 280, 339, 681
Sau96I GGNCC 1 cut(s) 457
SchI GAGTC 2 cut(s) 6, 64
ScrFI CCNGG 1 cut(s) 563
SduI GDGCHC 1 cut(s) 471
SetI ASST 6 cut(s) 21, 263, 294, 385, 456, 680
SfaNI GCATC 1 cut(s) 229
SfuI TTCGAA 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 3 cut(s) 327, 553, 590
SsiI CCGC 3 cut(s) 39, 100, 418
SspI AATATT 1 cut(s) 632
SspMI CTAG 2 cut(s) 318, 501
StyD4I CCNGG 1 cut(s) 561
TaaI ACNGT 1 cut(s) 642
TaqI TCGA 1 cut(s) 137
TasI AATT 3 cut(s) 327, 553, 590
TatI WGTACW 1 cut(s) 89
TauI GCSGC 1 cut(s) 420
TfiI GAWTC 2 cut(s) 134, 427
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 1 cut(s) 552
TseFI GTSAC 1 cut(s) 543
TseI GCWGC 2 cut(s) 152, 155
Tsp45I GTSAC 1 cut(s) 543
TspDTI ATGAA 5 cut(s) 359, 387, 426, 489, 546
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 1 cut(s) 552
XapI RAATTY 1 cut(s) 553
XbaI TCTAGA 1 cut(s) 500
XspI CTAG 2 cut(s) 318, 501
Zsp2I ATGCAT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.