MD17G1031900.v1.1

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000338
Physical Location & Seq
Reverse (-)
2309792 .. 2315230
5439 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1031900.v1.1.491

Sequence Viewer

Length: 1788 bp
ATGGATGGTGAAGACAGTAATGATTTTGACTGGAATACTGATGATGAGCTGGAAATTGAGAACTTTACGTTGTCTACTTCTTCAAGTTTGACAGTTGGTGGAGAAGCTATTGTGAGATCTGGGGAGTCGAGCTCATCGGCCGGTCCCTCCAATACCAAAGTTCTTGATCACTTTGTGGCGATGGGATTTTCTGAAAAGATGGTTGTCAAAGCAATTCAGGAACATGGAGAAGAAAATACAGATTCAATTCTGGAAACCCTCCTCACTTATTCGGCTCTTGAGAGTTCTCCTGAAGAAAAGCAGGAAGTAGAAACGCTCCAGGAAGTAGAACCACAGGAAGTAGAACCGCAGGAAGCTGATTCCGATGACTTCTCTCTTGATGATTACGATGAGAGCTTTCTGGATGATTTTTCAGACTTGGATAGCTGTTCTGATACCGAGGAAATCATAAATCCTACATCGGAGAAAGGGAAGATACGGTCCTTGGTCAATATGGGGTACACAATGGAGGAGGCCTCAATAGCAATCGAAAGATGTGGTACAGACTCCACTGTTGTTGAGTTGACTGATTTTATATGTGCTGCTCAAATGGCACGGGCGGAAGATGCCCTTCTTCCGATTGAAGAGAAGCCTGGATCACATTCATACAAGAAGAGGAAACTTCTCGAGTATGAGATCCTAAAGAAGAAGAAGAGGCAGATGCGGCTTTGTGGAAACCAAGGGGATGATGATGAGACGGTTCATCTCCCAAATCCAATGGTTGGGTTTGGCACCCCAACTGATCCATATGTGCTGACACATAGGACTCTCCCAGAGGCAGCTGTAGGGCCTCCATACTTCTATTATGAGAATGTCGCACTAGCTCCTAAAGGGGTTTGGACCACCATCTCCCGCTTCCTGTATGATGTGCAGCCGGAGTTTGTGGACTCAAAGTACTTTTCTGCTGCTGCAAGGAAGAGAGGTTATGTCCACAACCTCCCCATCCAGAATAGGTTTCCTCTCATGCCACAGCCCCCAGACACCATAGCCAAGGCATTTCCCTTGACAAGGAAATGGTGGCCTTCTTGGGACAATCGGGAAAAGCTAAACTGCTTGCAAACATGTATTGCAAGTGCTAAGTTGACTGAGAGGATCAGGAAGGCTATTGAGCAATTTGATGGGGAACCACCAGAGAGAGTCCAGAAGTATGTCCTTGACGAATGCCGCAAGTGGAATCTGGTGTGGGTAGGGAGAAATAAGGTCGCCCCACTTGAGCCTGATGAAGTGGAGATGCTTTTGGGGTTCCCAAAAAACCACACAAGGGGAATAAGCCGGACTGACAGGTACAAGTCACTTGGGAATTCCTTCCAGGTTGACACTGTGGCATATCATCTGTCTGTGCTCAAGGATATGTATCCCAATGGCATCAACGTCCTCTCCCTCTTTACTGGCATTGGAGGGGCAGAAGTAGCCCTTCACCGGCTTGGTGTCCCTCTGAAGAATGTTATCTCTGTAGAGATCTCAGAAGTGAACAGAAACGTTGTGAGCAGTTGGTGGGAGCAAACCAACCAGAGAGGGAGGTTGCAGCACGTTGATGATGTGCAGCAACTGAATGGTGATCACCTTGAGCAGTACATGAGCGAGTTTGGCGGATTTGATCTGGTGATTGGTGGGAGCCCGTGCAACAATCTAGCAGGCAGCAATCGACATCATCGTGATGGACTGGAGGGTAAGGAGTCGGCCCTTTTTTATGACTTTTTCCGAATATTGGACTTGGTGAAGAACATGATGGGGGGAAGAAACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003886 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006346 GO:0006355 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008757 GO:0009008 GO:0009605 GO:0009607 GO:0009620 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018022 GO:0018193 GO:0018205 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032776 GO:0034284 GO:0034285 GO:0034641 GO:0034968 GO:0036211 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051704 GO:0051707 GO:0060255 GO:0061647 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090116 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1901564 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_methylase

Protein Analysis

596

Amino Acids

67.11

Weight (kDa)

4.77

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 470 - 584 1e-08 C-5 cytosine-specific DNA methylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14620
fragaria_vesca FvH4_6g30300 FvH4_6g30450 FvH4_6g50980 FvH4_6g50980
malus_domestica MD09G1029900.v1.1 MD17G1031900.v1.1
prunus_persica Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.3G287400_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1 Prupe.8G038800_v2.0.a1
pyrus_communis pycom111g02390 pycom17g02950
rosa_chinensis RchiOBHm_Chr1g0321581 RchiOBHm_Chr1g0321601 RchiOBHm_Chr1g0322481 RchiOBHm_Chr1g0322501 RchiOBHm_Chr1g0322511 RchiOBHm_Chr1g0322631 RchiOBHm_Chr1g0322691 RchiOBHm_Chr1g0322751 RchiOBHm_Chr1g0323101 RchiOBHm_Chr1g0323111 RchiOBHm_Chr1g0328981 RchiOBHm_Chr1g0328991 RchiOBHm_Chr2g0136781 RchiOBHm_Chr2g0136791 RchiOBHm_Chr2g0136801 RchiOBHm_Chr2g0172381
rosa_laevigata RLG00000019575 RLG00000019577 RLG00000030366 RLG00000030370 RLG00000030377 RLG00000030379
rosa_multiflora Rmu_co8075278.1_g000001 Rmu_co8206584.1_g000001 Rmu_sc0000467.1_g000002 Rmu_sc0000467.1_g000007 Rmu_sc0001299.1_g000016 Rmu_sc0001932.1_g000013 Rmu_sc0002132.1_g000049 Rmu_sc0005093.1_g000002 Rmu_sc0005949.1_g000027 Rmu_sc0007485.1_g000011 Rmu_sc0007799.1_g000006 Rmu_sc0023751.1_g000001 Rmu_sc0039575.1_g000001
rosa_roxburghii Rroxscaffold_2G00107990 Rroxscaffold_2G00129580 Rroxscaffold_4G00326990
rosa_rugosa Rorug01G0034500 Rorug01G0035900 Rorug01G0036600 Rorug02G0268000 Rorug02G0333200 Rorug02G0333200 Rorug02G0333300 Rorug02G0333300 Rorug02G0333400 Rorug02G0333500 Rorug02G0333600 Rorug02G0333700 Rorug02G0333700 Rorug02G0333700 Rorug02G0558900 Rorug02G0559000 Rorug06G0043800 Rorug06G0044300
rosa_samantha Rh1AG051000 Rh1AG052400 Rh1AG052600 Rh1AG053200 Rh1AG053300 Rh1AG054000 Rh1BG046600 Rh1BG109100 Rh1CG048300 Rh1CG053000 Rh1CG053100 Rh1DG057000 Rh2AG384300 Rh2AG634100 Rh2BG390900 Rh2BG391000 Rh2BG647800 Rh2BG648200 Rh2CG370600 Rh2CG370800 Rh2CG614800 Rh2DG407600 Rh2DG407700 Rh2DG662800 Rh7BG379000
rosa_wichuraiana Rw1G004090 Rw1G004430 Rw2G031410 Rw2G031420 Rw2G052520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 770
AccB7I CCANNNNNTGG 1 cut(s) 761
AccI GTMKAC 1 cut(s) 74
AciI CCGC 6 cut(s) 347, 599, 703, 892, 1204, 1629
AclI AACGTT 1 cut(s) 1518
AclWI GGATC 4 cut(s) 643, 670, 776, 1139
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 1 cut(s) 1339
AcuI CTGAAG 2 cut(s) 312, 1496
AdeI CACNNNGTG 1 cut(s) 175
AfaI GTAC 5 cut(s) 500, 541, 935, 1325, 1613
AfiI CCNNNNNNNGG 5 cut(s) 761, 1047, 1300, 1458, 1747
AflIII ACRYGT 1 cut(s) 1100
AgsI TTSAA 3 cut(s) 84, 246, 623
AjnI CCWGG 3 cut(s) 318, 631, 1347
AluBI AGCT 9 cut(s) 49, 107, 132, 356, 396, 426, 821, 863, 1084
AluI AGCT 9 cut(s) 49, 107, 132, 356, 396, 426, 821, 863, 1084
Alw21I GWGCWC 2 cut(s) 134, 1383
Alw26I GTCTC 1 cut(s) 728
AlwI GGATC 4 cut(s) 643, 670, 776, 1139
AlwNI CAGNNNCTG 1 cut(s) 1588
Ama87I CYCGRG 1 cut(s) 665
AoxI GGCC 5 cut(s) 138, 513, 827, 1058, 1719
ApeKI GCWGC 8 cut(s) 581, 818, 910, 944, 947, 1564, 1582, 1677
ApoI RAATTY 1 cut(s) 1339
Asp700I GAANNNNTTC 1 cut(s) 1343
AspS9I GGNCC 5 cut(s) 143, 480, 827, 879, 1720
AsuHPI GGTGA 6 cut(s) 20, 1448, 1592, 1607, 1654, 1768
AvaI CYCGRG 1 cut(s) 665
AvaII GGWCC 3 cut(s) 143, 480, 879
BanI GGYRCC 1 cut(s) 770
BanII GRGCYC 2 cut(s) 134, 1658
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 2 cut(s) 134, 1383
BbvI GCAGC 8 cut(s) 568, 830, 922, 931, 934, 1576, 1594, 1689
BccI CCATC 7 cut(s) 175, 193, 893, 989, 1151, 1691, 1762
BciT130I CCWGG 3 cut(s) 320, 633, 1349
BciVI GTATCC 1 cut(s) 1404
BclI TGATCA 2 cut(s) 166, 1597
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 2 cut(s) 860, 1670
BfmI CTRYAG 2 cut(s) 822, 1491
BfuI GTATCC 1 cut(s) 1404
BglII AGATCT 2 cut(s) 116, 1497
BmcAI AGTACT 1 cut(s) 935
Bme1390I CCNGG 3 cut(s) 320, 633, 1349
Bme18I GGWCC 3 cut(s) 143, 480, 879
BmeT110I CYCGRG 1 cut(s) 665
BmgT120I GGNCC 5 cut(s) 143, 480, 827, 879, 1720
BmiI GGNNCC 5 cut(s) 145, 772, 1164, 1283, 1655
BmrFI CCNGG 3 cut(s) 320, 633, 1349
BmsI GCATC 4 cut(s) 595, 690, 1260, 1413
BpiI GAAGAC 1 cut(s) 18
BplI GAGNNNNNCTC 4 cut(s) 116, 148, 500, 532
BpmI CTGGAG 2 cut(s) 302, 1724
BpuEI CTTGAG 4 cut(s) 299, 1271, 1367, 1625
BsaBI GATNNNNATC 1 cut(s) 1392
BsaJI CCNNGG 4 cut(s) 438, 483, 718, 1029
BsaXI ACNNNNNCTCC 2 cut(s) 1400, 1430
Bsc4I CCNNNNNNNGG 5 cut(s) 761, 1047, 1300, 1458, 1747
Bse118I RCCGGY 2 cut(s) 140, 1458
Bse1I ACTGG 3 cut(s) 35, 1432, 1707
Bse8I GATNNNNATC 1 cut(s) 1392
BseBI CCWGG 3 cut(s) 320, 633, 1349
BseDI CCNNGG 4 cut(s) 438, 483, 718, 1029
BseGI GGATG 4 cut(s) 10, 409, 730, 981
BseJI GATNNNNATC 1 cut(s) 1392
BseLI CCNNNNNNNGG 5 cut(s) 761, 1047, 1300, 1458, 1747
BseMII CTCAG 2 cut(s) 1116, 1515
BseNI ACTGG 3 cut(s) 35, 1432, 1707
BseRI GAGGAG 2 cut(s) 251, 524
BseX3I CGGCCG 1 cut(s) 138
BseXI GCAGC 8 cut(s) 568, 830, 922, 931, 934, 1576, 1594, 1689
BsgI GTGCAG 2 cut(s) 929, 1601
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 5 cut(s) 140, 515, 829, 1060, 1721
BshNI GGYRCC 1 cut(s) 770
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 134, 1383
BsiHKCI CYCGRG 1 cut(s) 665
BsiSI CCGG 4 cut(s) 141, 914, 1312, 1459
BslFI GGGAC 3 cut(s) 129, 1082, 1454
BslI CCNNNNNNNGG 5 cut(s) 761, 1047, 1300, 1458, 1747
BsmAI GTCTC 1 cut(s) 728
BsmBI CGTCTC 1 cut(s) 728
BsmFI GGGAC 3 cut(s) 129, 1082, 1454
BsmI GAATGC 1 cut(s) 1205
BsnI GGCC 5 cut(s) 140, 515, 829, 1060, 1721
BsoBI CYCGRG 1 cut(s) 665
Bsp1286I GDGCHC 3 cut(s) 134, 1383, 1658
Bsp143I GATC 9 cut(s) 116, 166, 635, 675, 781, 1131, 1497, 1597, 1636
BspACI CCGC 6 cut(s) 347, 599, 703, 892, 1204, 1629
BspANI GGCC 5 cut(s) 140, 515, 829, 1060, 1721
BspCNI CTCAG 2 cut(s) 1117, 1514
BspLI GGNNCC 5 cut(s) 145, 772, 1164, 1283, 1655
BspPI GGATC 4 cut(s) 643, 670, 776, 1139
BspT107I GGYRCC 1 cut(s) 770
BsrFI RCCGGY 2 cut(s) 140, 1458
BsrI ACTGG 3 cut(s) 35, 1432, 1707
BssAI RCCGGY 2 cut(s) 140, 1458
BssECI CCNNGG 4 cut(s) 438, 483, 718, 1029
BssMI GATC 9 cut(s) 116, 166, 635, 675, 781, 1131, 1497, 1597, 1636
BssT1I CCWWGG 3 cut(s) 483, 718, 1029
Bst2UI CCWGG 3 cut(s) 320, 633, 1349
Bst4CI ACNGT 6 cut(s) 17, 94, 480, 553, 739, 1360
Bst6I CTCTTC 4 cut(s) 618, 647, 686, 950
BstC8I GCNNGC 2 cut(s) 1094, 1675
BstDEI CTNAG 3 cut(s) 1116, 1125, 1501
BstENI CCTNNNNNAGG 1 cut(s) 1045
BstF5I GGATG 4 cut(s) 10, 409, 730, 981
BstKTI GATC 9 cut(s) 119, 169, 638, 678, 784, 1134, 1500, 1600, 1639
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 9 cut(s) 116, 166, 635, 675, 781, 1131, 1497, 1597, 1636
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 6 cut(s) 521, 590, 605, 703, 1448, 1626
BstNI CCWGG 3 cut(s) 320, 633, 1349
BstNSI RCATGY 1 cut(s) 1104
BstSCI CCNGG 3 cut(s) 318, 631, 1347
BstSFI CTRYAG 2 cut(s) 822, 1491
BstV1I GCAGC 8 cut(s) 568, 830, 922, 931, 934, 1576, 1594, 1689
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 3 cut(s) 116, 675, 1497
BstYI RGATCY 3 cut(s) 116, 675, 1497
BstZI CGGCCG 1 cut(s) 138
BsuI GTATCC 1 cut(s) 1404
BsuRI GGCC 5 cut(s) 140, 515, 829, 1060, 1721
BtgZI GCGATG 1 cut(s) 194
BtsCI GGATG 4 cut(s) 10, 409, 730, 981
BtsIMutI CAGTG 2 cut(s) 549, 1356
Cac8I GCNNGC 2 cut(s) 1094, 1675
CaiI CAGNNNCTG 1 cut(s) 1588
Cfr10I RCCGGY 2 cut(s) 140, 1458
Cfr13I GGNCC 5 cut(s) 143, 480, 827, 879, 1720
Csp6I GTAC 5 cut(s) 499, 540, 934, 1324, 1612
CspCI CAANNNNNGTGG 2 cut(s) 538, 573
CviAII CATG 5 cut(s) 224, 1003, 1101, 1615, 1765
CviQI GTAC 5 cut(s) 499, 540, 934, 1324, 1612
DdeI CTNAG 3 cut(s) 1116, 1125, 1501
DpnI GATC 9 cut(s) 118, 168, 637, 677, 783, 1133, 1499, 1599, 1638
DpnII GATC 9 cut(s) 116, 166, 635, 675, 781, 1131, 1497, 1597, 1636
DraIII CACNNNGTG 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 138
EagI CGGCCG 1 cut(s) 138
Eam1104I CTCTTC 4 cut(s) 618, 647, 686, 950
EarI CTCTTC 4 cut(s) 618, 647, 686, 950
EciI GGCGGA 2 cut(s) 614, 1644
Ecl136II GAGCTC 1 cut(s) 132
EclXI CGGCCG 1 cut(s) 138
Eco130I CCWWGG 3 cut(s) 483, 718, 1029
Eco147I AGGCCT 1 cut(s) 515
Eco24I GRGCYC 2 cut(s) 134, 1658
Eco47I GGWCC 3 cut(s) 143, 480, 879
Eco52I CGGCCG 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 132
Eco57I CTGAAG 2 cut(s) 312, 1496
Eco88I CYCGRG 1 cut(s) 665
EcoICRI GAGCTC 1 cut(s) 132
EcoNI CCTNNNNNAGG 1 cut(s) 1045
EcoO109I RGGNCCY 1 cut(s) 827
EcoRI GAATTC 1 cut(s) 1339
EcoRII CCWGG 3 cut(s) 318, 631, 1347
EcoT14I CCWWGG 3 cut(s) 483, 718, 1029
EcoT38I GRGCYC 2 cut(s) 134, 1658
ErhI CCWWGG 3 cut(s) 483, 718, 1029
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 5 cut(s) 227, 1006, 1104, 1618, 1768
FalI AAGNNNNNCTT 4 cut(s) 594, 626, 1437, 1469
FaqI GGGAC 3 cut(s) 129, 1082, 1454
FatI CATG 5 cut(s) 223, 1002, 1100, 1614, 1764
FauI CCCGC 1 cut(s) 899
FauNDI CATATG 1 cut(s) 787
FbaI TGATCA 2 cut(s) 166, 1597
FblI GTMKAC 1 cut(s) 74
FokI GGATG 4 cut(s) 17, 416, 737, 968
FriOI GRGCYC 2 cut(s) 134, 1658
FspBI CTAG 2 cut(s) 860, 1670
GsuI CTGGAG 2 cut(s) 302, 1724
HaeIII GGCC 5 cut(s) 140, 515, 829, 1060, 1721
HapII CCGG 4 cut(s) 141, 914, 1312, 1459
Hin1II CATG 5 cut(s) 227, 1006, 1104, 1618, 1768
HincII GTYRAC 3 cut(s) 564, 1122, 1354
HindII GTYRAC 3 cut(s) 564, 1122, 1354
HinfI GANTC 9 cut(s) 125, 242, 359, 545, 805, 926, 1176, 1213, 1715
HpaII CCGG 4 cut(s) 141, 914, 1312, 1459
HphI GGTGA 6 cut(s) 20, 1448, 1592, 1607, 1654, 1768
Hpy166II GTNNAC 8 cut(s) 75, 501, 564, 925, 970, 1122, 1354, 1510
Hpy188I TCNGA 9 cut(s) 193, 364, 415, 433, 463, 618, 1476, 1504, 1742
Hpy8I GTNNAC 8 cut(s) 75, 501, 564, 925, 970, 1122, 1354, 1510
HpyAV CCTTC 5 cut(s) 620, 1071, 1132, 1354, 1463
HpyCH4III ACNGT 6 cut(s) 17, 94, 480, 553, 739, 1360
HpyCH4IV ACGT 4 cut(s) 68, 1410, 1518, 1569
HpyCH4V TGCA 7 cut(s) 910, 950, 1096, 1109, 1564, 1582, 1662
HpyF10VI GCNNNNNNNGC 6 cut(s) 521, 590, 605, 703, 1448, 1626
HpyF3I CTNAG 3 cut(s) 1116, 1125, 1501
HpySE526I ACGT 4 cut(s) 68, 1410, 1518, 1569
Hsp92II CATG 5 cut(s) 227, 1006, 1104, 1618, 1768
Ksp22I TGATCA 2 cut(s) 166, 1597
Kzo9I GATC 9 cut(s) 116, 166, 635, 675, 781, 1131, 1497, 1597, 1636
LmnI GCTCC 4 cut(s) 321, 868, 1537, 1653
Lsp1109I GCAGC 8 cut(s) 568, 830, 922, 931, 934, 1576, 1594, 1689
LweI GCATC 4 cut(s) 595, 690, 1260, 1413
MaeI CTAG 2 cut(s) 860, 1670
MaeII ACGT 4 cut(s) 68, 1410, 1518, 1569
MaeIII GTNAC 1 cut(s) 1329
MalI GATC 9 cut(s) 118, 168, 637, 677, 783, 1133, 1499, 1599, 1638
MboI GATC 9 cut(s) 116, 166, 635, 675, 781, 1131, 1497, 1597, 1636
MfeI CAATTG 1 cut(s) 1783
MflI RGATCY 3 cut(s) 116, 675, 1497
MhlI GDGCHC 3 cut(s) 134, 1383, 1658
MluCI AATT 6 cut(s) 54, 213, 246, 1151, 1339, 1783
MlyI GAGTC 6 cut(s) 134, 539, 799, 920, 1185, 1724
MroXI GAANNNNTTC 1 cut(s) 1343
MslI CAYNNNNRTG 3 cut(s) 1572, 1692, 1695
MspA1I CMGCKG 1 cut(s) 821
MspI CCGG 4 cut(s) 141, 914, 1312, 1459
MspR9I CCNGG 3 cut(s) 320, 633, 1349
MunI CAATTG 1 cut(s) 1783
Mva1269I GAATGC 1 cut(s) 1205
MvaI CCWGG 3 cut(s) 320, 633, 1349
MwoI GCNNNNNNNGC 6 cut(s) 521, 590, 605, 703, 1448, 1626
NdeI CATATG 1 cut(s) 787
NdeII GATC 9 cut(s) 116, 166, 635, 675, 781, 1131, 1497, 1597, 1636
NlaIII CATG 5 cut(s) 227, 1006, 1104, 1618, 1768
NlaIV GGNNCC 5 cut(s) 145, 772, 1164, 1283, 1655
NmuCI GTSAC 1 cut(s) 1329
NspI RCATGY 1 cut(s) 1104
PaeR7I CTCGAG 1 cut(s) 665
PceI AGGCCT 1 cut(s) 515
PciI ACATGT 1 cut(s) 1100
PcsI WCGNNNNNNNCGW 1 cut(s) 1690
PctI GAATGC 1 cut(s) 1205
PdmI GAANNNNTTC 1 cut(s) 1343
PfeI GAWTC 3 cut(s) 242, 359, 1213
PflMI CCANNNNNTGG 1 cut(s) 761
PfoI TCCNGGA 1 cut(s) 318
PleI GAGTC 6 cut(s) 133, 539, 799, 920, 1184, 1723
PpsI GAGTC 6 cut(s) 133, 539, 799, 920, 1184, 1723
PscI ACATGT 1 cut(s) 1100
Psp124BI GAGCTC 1 cut(s) 134
Psp1406I AACGTT 1 cut(s) 1518
Psp6I CCWGG 3 cut(s) 318, 631, 1347
PspGI CCWGG 3 cut(s) 318, 631, 1347
PspN4I GGNNCC 5 cut(s) 145, 772, 1164, 1283, 1655
PspPI GGNCC 5 cut(s) 143, 480, 827, 879, 1720
PstNI CAGNNNCTG 1 cut(s) 1588
PsuI RGATCY 3 cut(s) 116, 675, 1497
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 5 cut(s) 500, 541, 935, 1325, 1613
RsaNI GTAC 5 cut(s) 499, 540, 934, 1324, 1612
RseI CAYNNNNRTG 3 cut(s) 1572, 1692, 1695
SacI GAGCTC 1 cut(s) 134
Sau3AI GATC 9 cut(s) 116, 166, 635, 675, 781, 1131, 1497, 1597, 1636
Sau96I GGNCC 5 cut(s) 143, 480, 827, 879, 1720
ScaI AGTACT 1 cut(s) 935
SchI GAGTC 6 cut(s) 134, 539, 799, 920, 1185, 1724
ScrFI CCNGG 3 cut(s) 320, 633, 1349
SduI GDGCHC 3 cut(s) 134, 1383, 1658
SfaNI GCATC 4 cut(s) 595, 690, 1260, 1413
SfcI CTRYAG 2 cut(s) 822, 1491
Sfr274I CTCGAG 1 cut(s) 665
SinI GGWCC 3 cut(s) 143, 480, 879
SlaI CTCGAG 1 cut(s) 665
SmiMI CAYNNNNRTG 3 cut(s) 1572, 1692, 1695
SmlI CTYRAG 5 cut(s) 278, 665, 1250, 1382, 1604
SmoI CTYRAG 5 cut(s) 278, 665, 1250, 1382, 1604
Sse9I AATT 6 cut(s) 54, 213, 246, 1151, 1339, 1783
SseBI AGGCCT 1 cut(s) 515
SsiI CCGC 6 cut(s) 347, 599, 703, 892, 1204, 1629
SspI AATATT 1 cut(s) 1746
SspMI CTAG 2 cut(s) 860, 1670
SstI GAGCTC 1 cut(s) 134
StuI AGGCCT 1 cut(s) 515
StyD4I CCNGG 3 cut(s) 318, 631, 1347
StyI CCWWGG 3 cut(s) 483, 718, 1029
TaaI ACNGT 6 cut(s) 17, 94, 480, 553, 739, 1360
TaiI ACGT 4 cut(s) 71, 1413, 1521, 1572
TaqI TCGA 4 cut(s) 128, 528, 666, 1684
TasI AATT 6 cut(s) 54, 213, 246, 1151, 1339, 1783
TatI WGTACW 2 cut(s) 933, 1611
TauI GCSGC 2 cut(s) 706, 1206
TfiI GAWTC 3 cut(s) 242, 359, 1213
TscAI CASTG 2 cut(s) 556, 1363
TseFI GTSAC 1 cut(s) 1329
TseI GCWGC 8 cut(s) 581, 818, 910, 944, 947, 1564, 1582, 1677
Tsp45I GTSAC 1 cut(s) 1329
TspDTI ATGAA 3 cut(s) 633, 731, 1275
TspRI CASTG 2 cut(s) 556, 1363
Van91I CCANNNNNTGG 1 cut(s) 761
VpaK11BI GGWCC 3 cut(s) 143, 480, 879
XagI CCTNNNNNAGG 1 cut(s) 1045
XapI RAATTY 1 cut(s) 1339
XceI RCATGY 1 cut(s) 1104
XhoI CTCGAG 1 cut(s) 665
XmiI GTMKAC 1 cut(s) 74
XmnI GAANNNNTTC 1 cut(s) 1343
XspI CTAG 2 cut(s) 860, 1670
ZrmI AGTACT 1 cut(s) 935
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.