FvH4_6g43941

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000449
Physical Location & Seq
Reverse (-)
33935386 .. 33936687
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43941.t1

Sequence Viewer

Length: 1302 bp
ATGTCGAAGTGCTCAGAAGCAGCAGAGGCCGTAGCCAATATCGAAGAGCTCCTCACGCAGATCCTTGAGTCAGTGCCATTACAATCTCTTACGCGTTTCAAGTGCGTCTCCAAGCATTGGCAATCTCTCATCTCCAACCTCCGCACCCTCAAATATCCTAAGATAACGTCTTTCGTCTCAAGCAAAACCGAAGCCGGGTCCTTCAAGAGCATTCCTATTGATCACCGTGAAATCCCATCGGGTTGGAATCCGTTCAAAACTTTTAACGATTCCTTCACCGATGGCTCCTGGTTGAAGATTATCCAGTCCTGCAATGGCCTCTTTCTATGCTGCGTTTCCCCCAGCATAGTAAGAAGAGAAAAGGATTTGATTGTAAGAAGACCAAATCGTGGCGTTACTGGCGTATTTGTTGTTAATCCCGCAACCAAAAGGTTCTTGGCTCTTTCTTCACCGACGTCATCTTCGTTTAACAGTTGGGAACGTTATGCTTTGGCTTTTGAACCTTCCAAATCTCCTCATTACACCTTGGTCTGTGTGACTACGCTCAATGGAACTCTCCACCAAAAAATACATATATATTCGTCTAAGACTCAAGAGTGGAAACATGTTGCTGATTTGCTTTCTTCCGAGCTGCGCTACTTTCGTTCTGATGAGAGAAGCAGGGAAGGCTCTGTGTACAGCAACGGCGCAGTTCATTGGATAAGAGATATAAATAGAGCAGGATTCCCTAAAAATAGATTATTTTTCTGCAAGAAAGCTGGTAACTTCTCTAGATTAAAATCTGATGTATTGCACTACTTTGACATAGGTGAAGAAAATTTTCTAGTCGTGGCTGCTACTCCTCCAGTTCCTACGGTTGTCAAGAACATACCGTTGAAGGAGTGTCGTAATCGTGGCCTCAAGACTCCCATTGTGTGGCCGATATTGGTTCATAGATACTTTGGGGATTGCGGTGGCCGTTTGTACTTGATTGAGGTTTATAGCCATTGTAACACTCAGTTTGATGTGATGGAGATGGGGAAGGACTACTCTGGTTGGTTTATCAAGTATCATGTTGATCTAAATCCGACGGCTCTTCCTCAATGTAATTTGGATCCGTTTGTTATATTGTGCCTTTCTCGTGAGAGGGAAGAAGAGTTCGGGGATCCTTCAACTACTATGATGTTGCATATGCCCGGTAAGGTCATATCTTATAATCTTAGGGATAAGAACTTCAAGACATCTTCTGTGTTAACCATAGAGGATACTAAAATGTATAAAGTCGGTTATTATCCCTATATGGAGACTCTGGCTTGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

434

Amino Acids

49.49

Weight (kDa)

9.02

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 15 - 49 5e-07 F-box domain
FBA_1 PF07734 100 - 278 9.6e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12890 FvH4_4g01370 FvH4_4g01420 FvH4_4g01430 FvH4_4g01472 FvH4_4g01490 FvH4_6g31140 FvH4_6g31150 FvH4_6g31161 FvH4_6g43941 FvH4_6g43961
rosa_chinensis RchiOBHm_Chr4g0387951 RchiOBHm_Chr4g0388001 RchiOBHm_Chr4g0388011 RchiOBHm_Chr4g0388051 RchiOBHm_Chr4g0388631 RchiOBHm_Chr4g0388641 RchiOBHm_Chr4g0388661 RchiOBHm_Chr4g0388801
rosa_laevigata RLG00000010028 RLG00000010030 RLG00000010032 RLG00000010082 RLG00000010086 RLG00000010089 RLG00000010091 RLG00000014277
rosa_multiflora Rmu_co8138914.1_g000001 Rmu_sc0000697.1_g000015 Rmu_sc0002180.1_g000030 Rmu_sc0002509.1_g000011 Rmu_sc0002509.1_g000012 Rmu_sc0002509.1_g000020 Rmu_sc0002509.1_g000021 Rmu_sc0004459.1_g000003 Rmu_sc0004459.1_g000005 Rmu_sc0006767.1_g000007 Rmu_sc0014755.1_g000003 Rmu_sc0014755.1_g000004 Rmu_sc0036097.1_g000001
rosa_roxburghii Rroxscaffold_2G00089440 Rroxscaffold_3G00220040 Rroxscaffold_5G00334500 Rroxscaffold_5G00334530 Rroxscaffold_5G00334540 Rroxscaffold_5G00335080
rosa_rugosa Rorug02G0485300 Rorug03G0314100 Rorug03G0314200 Rorug03G0314500 Rorug03G0314500 Rorug03G0314600 Rorug03G0319800 Rorug03G0319900 Rorug03G0320000 Rorug06G0011400
rosa_samantha Rh4AG019700 Rh4AG019900 Rh4AG020200 Rh4AG023900 Rh4AG024000 Rh4AG024700 Rh4BG013900 Rh4BG014100 Rh4BG014200 Rh4BG014300 Rh4BG014400 Rh4BG014500 Rh4BG014600 Rh4BG014700 Rh4BG017100 Rh4BG017400 Rh4BG017500 Rh4BG017600 Rh4BG017700 Rh4BG089200 Rh4DG014500 Rh4DG014800 Rh4DG014900 Rh4DG015400 Rh4DG017900 Rh4DG018000 Rh6BG127900 Rh6CG126300 Rh6DG113400
rosa_wichuraiana Rw4G001270 Rw4G001320 Rw4G001350 Rw4G001700 Rw4G001710 Rw4G001720 Rw4G001760 Rw6G011430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1194
AatII GACGTC 1 cut(s) 458
AccB7I CCANNNNNTGG 3 cut(s) 117, 389, 915
AccII CGCG 1 cut(s) 94
AciI CCGC 3 cut(s) 142, 420, 951
AclI AACGTT 1 cut(s) 481
AclWI GGATC 5 cut(s) 55, 1088, 1101, 1139, 1152
AcoI YGGCCR 2 cut(s) 917, 955
AcsI RAATTY 1 cut(s) 817
AcyI GRCGYC 1 cut(s) 455
AfaI GTAC 2 cut(s) 677, 965
AfiI CCNNNNNNNGG 4 cut(s) 117, 195, 389, 915
AflIII ACRYGT 2 cut(s) 92, 604
AgsI TTSAA 8 cut(s) 100, 205, 256, 295, 500, 877, 1152, 1216
AjnI CCWGG 1 cut(s) 287
AloI GAACNNNNNNTCC 2 cut(s) 1121, 1153
AluBI AGCT 3 cut(s) 49, 631, 758
AluI AGCT 3 cut(s) 49, 631, 758
Alw21I GWGCWC 2 cut(s) 14, 51
Alw26I GTCTC 3 cut(s) 112, 181, 1277
AlwI GGATC 5 cut(s) 55, 1088, 1101, 1139, 1152
AoxI GGCC 5 cut(s) 27, 316, 895, 917, 955
ApeKI GCWGC 4 cut(s) 20, 330, 631, 833
ApoI RAATTY 1 cut(s) 817
ArsI GACNNNNNNTTYG 2 cut(s) 445, 477
Asp700I GAANNNNTTC 2 cut(s) 251, 819
AspLEI GCGC 2 cut(s) 636, 689
AspS9I GGNCC 1 cut(s) 198
AsuC2I CCSGG 2 cut(s) 196, 1176
AsuHPI GGTGA 4 cut(s) 215, 268, 441, 821
AvaII GGWCC 1 cut(s) 198
BamHI GGATCC 2 cut(s) 1093, 1144
BanII GRGCYC 1 cut(s) 51
BauI CACGAG 1 cut(s) 1119
BbsI GAAGAC 1 cut(s) 385
Bbv12I GWGCWC 2 cut(s) 14, 51
BbvI GCAGC 4 cut(s) 32, 317, 618, 820
BccI CCATC 4 cut(s) 244, 275, 1003, 1009
BceAI ACGGC 4 cut(s) 14, 700, 942, 1086
BciT130I CCWGG 1 cut(s) 289
BciVI GTATCC 1 cut(s) 1237
BclI TGATCA 1 cut(s) 220
BcnI CCSGG 2 cut(s) 196, 1176
BcoDI GTCTC 3 cut(s) 112, 181, 1277
BfaI CTAG 2 cut(s) 771, 824
BfuI GTATCC 1 cut(s) 1237
BisI GCNGC 4 cut(s) 21, 331, 632, 834
BlsI GCNGC 4 cut(s) 22, 332, 633, 835
Bme1390I CCNGG 3 cut(s) 196, 289, 1176
Bme18I GGWCC 1 cut(s) 198
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 4 cut(s) 199, 286, 1095, 1146
BmrFI CCNGG 3 cut(s) 196, 289, 1176
BpiI GAAGAC 1 cut(s) 385
BpmI CTGGAG 1 cut(s) 828
BpuEI CTTGAG 4 cut(s) 86, 163, 576, 884
BpuMI CCSGG 2 cut(s) 196, 1176
BsaBI GATNNNNATC 2 cut(s) 778, 1062
BsaHI GRCGYC 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 525
Bsc4I CCNNNNNNNGG 4 cut(s) 117, 195, 389, 915
Bse1I ACTGG 3 cut(s) 304, 403, 845
Bse3DI GCAATG 1 cut(s) 319
Bse8I GATNNNNATC 2 cut(s) 778, 1062
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 1 cut(s) 525
BseJI GATNNNNATC 2 cut(s) 778, 1062
BseLI CCNNNNNNNGG 4 cut(s) 117, 195, 389, 915
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 2 cut(s) 27, 1010
BseNI ACTGG 3 cut(s) 304, 403, 845
BseRI GAGGAG 3 cut(s) 41, 504, 831
BseXI GCAGC 4 cut(s) 32, 317, 618, 820
BseYI CCCAGC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 94
BshFI GGCC 5 cut(s) 29, 318, 897, 919, 957
BsiHKAI GWGCWC 2 cut(s) 14, 51
BsiSI CCGG 2 cut(s) 195, 1176
BslI CCNNNNNNNGG 4 cut(s) 117, 195, 389, 915
BsmAI GTCTC 3 cut(s) 112, 181, 1277
BsmBI CGTCTC 2 cut(s) 112, 181
BsmI GAATGC 1 cut(s) 210
BsnI GGCC 5 cut(s) 29, 318, 897, 919, 957
Bsp1286I GDGCHC 2 cut(s) 14, 51
Bsp1407I TGTACA 1 cut(s) 675
Bsp143I GATC 5 cut(s) 60, 220, 1057, 1093, 1144
BspACI CCGC 3 cut(s) 142, 420, 951
BspANI GGCC 5 cut(s) 29, 318, 897, 919, 957
BspCNI CTCAG 2 cut(s) 26, 1009
BspFNI CGCG 1 cut(s) 94
BspLI GGNNCC 4 cut(s) 199, 286, 1095, 1146
BspPI GGATC 5 cut(s) 55, 1088, 1101, 1139, 1152
BspQI GCTCTTC 2 cut(s) 39, 1080
BsrDI GCAATG 1 cut(s) 319
BsrGI TGTACA 1 cut(s) 675
BsrI ACTGG 3 cut(s) 304, 403, 845
BssECI CCNNGG 1 cut(s) 525
BssMI GATC 5 cut(s) 60, 220, 1057, 1093, 1144
BssNI GRCGYC 1 cut(s) 455
BssSI CACGAG 1 cut(s) 1119
BssT1I CCWWGG 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 1119
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 4 cut(s) 227, 473, 856, 873
Bst6I CTCTTC 4 cut(s) 39, 349, 1080, 1128
BstACI GRCGYC 1 cut(s) 455
BstAUI TGTACA 1 cut(s) 675
BstDEI CTNAG 5 cut(s) 13, 159, 585, 996, 1199
BstFNI CGCG 1 cut(s) 94
BstHHI GCGC 2 cut(s) 636, 689
BstKTI GATC 5 cut(s) 63, 223, 1060, 1096, 1147
BstMAI GTCTC 3 cut(s) 112, 181, 1277
BstMBI GATC 5 cut(s) 60, 220, 1057, 1093, 1144
BstMWI GCNNNNNNNGC 4 cut(s) 26, 55, 399, 666
BstNI CCWGG 1 cut(s) 289
BstNSI RCATGY 1 cut(s) 608
BstSCI CCNGG 3 cut(s) 194, 287, 1174
BstUI CGCG 1 cut(s) 94
BstV1I GCAGC 4 cut(s) 32, 317, 618, 820
BstV2I GAAGAC 1 cut(s) 385
BstX2I RGATCY 3 cut(s) 60, 1093, 1144
BstXI CCANNNNNNTGG 1 cut(s) 243
BstYI RGATCY 3 cut(s) 60, 1093, 1144
BsuI GTATCC 1 cut(s) 1237
BsuRI GGCC 5 cut(s) 29, 318, 897, 919, 957
BtsIMutI CAGTG 1 cut(s) 78
CfoI GCGC 2 cut(s) 636, 689
Cfr13I GGNCC 1 cut(s) 198
CseI GACGC 1 cut(s) 94
Csp6I GTAC 2 cut(s) 676, 964
CviAII CATG 2 cut(s) 605, 1052
CviQI GTAC 2 cut(s) 676, 964
DdeI CTNAG 5 cut(s) 13, 159, 585, 996, 1199
DpnI GATC 5 cut(s) 62, 222, 1059, 1095, 1146
DpnII GATC 5 cut(s) 60, 220, 1057, 1093, 1144
EaeI YGGCCR 2 cut(s) 917, 955
Eam1104I CTCTTC 4 cut(s) 39, 349, 1080, 1128
EarI CTCTTC 4 cut(s) 39, 349, 1080, 1128
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 525
Eco24I GRGCYC 1 cut(s) 51
Eco47I GGWCC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 49
EcoICRI GAGCTC 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 198
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 525
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 525
Esp3I CGTCTC 2 cut(s) 112, 181
FaeI CATG 2 cut(s) 608, 1055
FatI CATG 2 cut(s) 604, 1051
FauI CCCGC 1 cut(s) 427
FauNDI CATATG 1 cut(s) 1170
FbaI TGATCA 1 cut(s) 220
Fnu4HI GCNGC 4 cut(s) 21, 331, 632, 834
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 4 cut(s) 21, 331, 632, 834
FspBI CTAG 2 cut(s) 771, 824
GlaI GCGC 2 cut(s) 635, 688
GluI GCNGC 4 cut(s) 21, 331, 632, 834
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 828
HaeIII GGCC 5 cut(s) 29, 318, 897, 919, 957
HapII CCGG 2 cut(s) 195, 1176
HgaI GACGC 1 cut(s) 94
HhaI GCGC 2 cut(s) 636, 689
Hin1I GRCGYC 1 cut(s) 455
Hin1II CATG 2 cut(s) 608, 1055
Hin6I GCGC 2 cut(s) 634, 687
HinP1I GCGC 2 cut(s) 634, 687
HincII GTYRAC 1 cut(s) 1233
HindII GTYRAC 1 cut(s) 1233
HinfI GANTC 7 cut(s) 68, 247, 269, 589, 723, 904, 1285
HpaI GTTAAC 1 cut(s) 1233
HpaII CCGG 2 cut(s) 195, 1176
HphI GGTGA 4 cut(s) 215, 268, 441, 821
Hpy166II GTNNAC 2 cut(s) 676, 1233
Hpy188I TCNGA 5 cut(s) 16, 628, 649, 784, 1068
Hpy188III TCNNGA 7 cut(s) 205, 593, 771, 862, 901, 1121, 1216
Hpy8I GTNNAC 2 cut(s) 676, 1233
Hpy99I CGWCG 2 cut(s) 457, 1072
HpyAV CCTTC 7 cut(s) 211, 283, 513, 659, 871, 1015, 1158
HpyCH4III ACNGT 4 cut(s) 227, 473, 856, 873
HpyCH4IV ACGT 3 cut(s) 167, 455, 481
HpyCH4V TGCA 4 cut(s) 312, 750, 793, 1168
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 55, 399, 666
HpyF3I CTNAG 5 cut(s) 13, 159, 585, 996, 1199
HpySE526I ACGT 3 cut(s) 167, 455, 481
Hsp92I GRCGYC 1 cut(s) 455
Hsp92II CATG 2 cut(s) 608, 1055
HspAI GCGC 2 cut(s) 634, 687
Ksp22I TGATCA 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 1233
Kzo9I GATC 5 cut(s) 60, 220, 1057, 1093, 1144
LguI GCTCTTC 2 cut(s) 39, 1080
LmnI GCTCC 2 cut(s) 54, 290
Lsp1109I GCAGC 4 cut(s) 32, 317, 618, 820
MaeI CTAG 2 cut(s) 771, 824
MaeII ACGT 3 cut(s) 167, 455, 481
MaeIII GTNAC 4 cut(s) 394, 535, 761, 989
MalI GATC 5 cut(s) 62, 222, 1059, 1095, 1146
MboI GATC 5 cut(s) 60, 220, 1057, 1093, 1144
MflI RGATCY 3 cut(s) 60, 1093, 1144
MhlI GDGCHC 2 cut(s) 14, 51
MluCI AATT 2 cut(s) 817, 1087
MluI ACGCGT 1 cut(s) 92
MlyI GAGTC 4 cut(s) 77, 583, 898, 1279
MmeI TCCRAC 3 cut(s) 159, 224, 1091
MroXI GAANNNNTTC 2 cut(s) 251, 819
MseI TTAA 5 cut(s) 264, 414, 468, 776, 1232
MspI CCGG 2 cut(s) 195, 1176
MspR9I CCNGG 3 cut(s) 196, 289, 1176
Mva1269I GAATGC 1 cut(s) 210
MvaI CCWGG 1 cut(s) 289
MvnI CGCG 1 cut(s) 94
MwoI GCNNNNNNNGC 4 cut(s) 26, 55, 399, 666
NciI CCSGG 2 cut(s) 196, 1176
NdeI CATATG 1 cut(s) 1170
NdeII GATC 5 cut(s) 60, 220, 1057, 1093, 1144
NlaIII CATG 2 cut(s) 608, 1055
NlaIV GGNNCC 4 cut(s) 199, 286, 1095, 1146
NmuCI GTSAC 1 cut(s) 535
NspI RCATGY 1 cut(s) 608
PciI ACATGT 1 cut(s) 604
PciSI GCTCTTC 2 cut(s) 39, 1080
PcsI WCGNNNNNNNCGW 1 cut(s) 461
PctI GAATGC 1 cut(s) 210
PdmI GAANNNNTTC 2 cut(s) 251, 819
PfeI GAWTC 3 cut(s) 247, 269, 723
PflMI CCANNNNNTGG 3 cut(s) 117, 389, 915
PkrI GCNGC 4 cut(s) 22, 332, 633, 835
PleI GAGTC 4 cut(s) 76, 583, 898, 1279
PpsI GAGTC 4 cut(s) 76, 583, 898, 1279
PpuMI RGGWCCY 1 cut(s) 198
PscI ACATGT 1 cut(s) 604
PsiI TTATAA 1 cut(s) 1194
Psp124BI GAGCTC 1 cut(s) 51
Psp1406I AACGTT 1 cut(s) 481
Psp5II RGGWCCY 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 287
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 4 cut(s) 199, 286, 1095, 1146
PspPI GGNCC 1 cut(s) 198
PspPPI RGGWCCY 1 cut(s) 198
PsuI RGATCY 3 cut(s) 60, 1093, 1144
RsaI GTAC 2 cut(s) 677, 965
RsaNI GTAC 2 cut(s) 676, 964
SacI GAGCTC 1 cut(s) 51
SapI GCTCTTC 2 cut(s) 39, 1080
SaqAI TTAA 5 cut(s) 264, 414, 468, 776, 1232
SatI GCNGC 4 cut(s) 21, 331, 632, 834
Sau3AI GATC 5 cut(s) 60, 220, 1057, 1093, 1144
Sau96I GGNCC 1 cut(s) 198
SchI GAGTC 4 cut(s) 77, 583, 898, 1279
ScrFI CCNGG 3 cut(s) 196, 289, 1176
SduI GDGCHC 2 cut(s) 14, 51
SinI GGWCC 1 cut(s) 198
SmlI CTYRAG 4 cut(s) 65, 178, 591, 899
SmoI CTYRAG 4 cut(s) 65, 178, 591, 899
Sse9I AATT 2 cut(s) 817, 1087
SsiI CCGC 3 cut(s) 142, 420, 951
SspMI CTAG 2 cut(s) 771, 824
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 3 cut(s) 194, 287, 1174
StyI CCWWGG 1 cut(s) 525
TaaI ACNGT 4 cut(s) 227, 473, 856, 873
TaiI ACGT 3 cut(s) 170, 458, 484
TaqI TCGA 2 cut(s) 5, 42
TasI AATT 2 cut(s) 817, 1087
TatI WGTACW 2 cut(s) 675, 963
TfiI GAWTC 3 cut(s) 247, 269, 723
Tru1I TTAA 5 cut(s) 264, 414, 468, 776, 1232
Tru9I TTAA 5 cut(s) 264, 414, 468, 776, 1232
TscAI CASTG 1 cut(s) 78
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 4 cut(s) 20, 330, 631, 833
Tsp45I GTSAC 1 cut(s) 535
TspDTI ATGAA 2 cut(s) 683, 920
TspGWI ACGGA 2 cut(s) 240, 1086
TspRI CASTG 1 cut(s) 78
Van91I CCANNNNNTGG 3 cut(s) 117, 389, 915
VpaK11BI GGWCC 1 cut(s) 198
XapI RAATTY 1 cut(s) 817
XbaI TCTAGA 1 cut(s) 770
XceI RCATGY 1 cut(s) 608
XcmI CCANNNNNNNNNTGG 2 cut(s) 311, 433
XmnI GAANNNNTTC 2 cut(s) 251, 819
XspI CTAG 2 cut(s) 771, 824
ZraI GACGTC 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.