Rh4BG014100

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000449
Physical Location & Seq
Reverse (-)
1976883 .. 1980072
3190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG014100.1

Sequence Viewer

Length: 1335 bp
ATGTCATCTTCAGCAGAAACGGTAGCGAACATCGAAGGGCTCCTTAAGCAGATCCTCATATCGGTTCCAGCTCCATCTCTGATCCGCTTCAAGTGCGTCTCCAAGCACTGGCTCTCTCTCATTTCCGACCCCGACTTCTGTCGCCGCCACACTCTCCGAAACCCTAACCCCAAAATCTCAGCTTTCTTCTCTTCCCAAACCAATGAAGACACCACCATCAACTCCATCACTCTCGGCAGCCCAGCCGGTAATCCCTTCAAAACTCTCGCCAATTCCGCCTCCGCGTTGAGGATTCTTCAGTCCTGCAATGGCCTCTTCCTCTGCTATGTCGAATTCCCCGGAGAAGAAGAAGAAAGAAACTATCCTCCTGTATATGTCGTCAATCCCACCACCAACAAATTCCGCGCTCTCTCTGCTCCAAGCCTTGCGAAGAAGGAGGAAGTGTATATGTTTGTGAGGTATGGTTTGGCTTTCGACCCTTCGAAATCGCCTCATTACACTGTGGTGTGTGTCAGTCACGACATTCTCCGTGGCAAGCGCCACAGCATAGAGATATATTCGTCGGAGACCGGAACTTGGGTGAAGCGTTTCAAGGCTCTTTTCTTCCCTAGCCCTTCCGACGAGGGCAGGCACATTGATACTCGCAGAATGCCTATGCATTTTGATGAGAGGAGCAGGGAAGGGGCAGTGTATTGCAACGGCGCAATTCATTGGATAAGAAACAGATGGGAAGCGAGCCTGCCATTCTGCTCGGAGACAGATGGTGTGGTCGAAACAGTTGGATTCGAAAGAAGTAAATTGGATGTGTTGCACTACTATGACATAGGTGAAGAAAGTCTGCGGCTCGCTGCTGCTACTCCTCCAGTTCCTTTGGTTGTGAAAAACATTCCGTTGGAGAAAGAACAAAACTTTCCTACTGAGTTTCCCCATTTGATTGATAGATACTTTGGCGAGTCAGGTGGTGGCTGTTTGTATTTGATTGAGGTTTTTGAGCATTGTAGGACTCAATTTGAGGTGATGGAGATGGAGAGGGACTACTCTGGCTGGTTTGTTAAGTACCATGTTGATCTTAGTCCGGTGGTCGCACCTCGTCCTGGACAGGACCCTAATGCTTTTGTTGTCTTGTTCCTTTCACGAGATGTGGAAACAGATGGTGAGGATTCTTCAACTGATCTTTGGTTGCATATGCCTGGTAAGGTCGTGTCTTATAATCTCAGGACTAAGAGCTTCAAGACATCTGTTGAGTTAGCCAATAGGGAACTTTTTCTAGCATTAGATGATGATGAATATGAGGGTGATAACTATCCTTACATGGAGACTTTAGCTTGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

444

Amino Acids

50.45

Weight (kDa)

5.47

Isoelectric Point (pI)

61.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 14 - 48 6.4e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12890 FvH4_4g01370 FvH4_4g01420 FvH4_4g01430 FvH4_4g01472 FvH4_4g01490 FvH4_6g31140 FvH4_6g31150 FvH4_6g31161 FvH4_6g43941 FvH4_6g43961
rosa_chinensis RchiOBHm_Chr4g0387951 RchiOBHm_Chr4g0388001 RchiOBHm_Chr4g0388011 RchiOBHm_Chr4g0388051 RchiOBHm_Chr4g0388631 RchiOBHm_Chr4g0388641 RchiOBHm_Chr4g0388661 RchiOBHm_Chr4g0388801
rosa_laevigata RLG00000010028 RLG00000010030 RLG00000010032 RLG00000010082 RLG00000010086 RLG00000010089 RLG00000010091 RLG00000014277
rosa_multiflora Rmu_co8138914.1_g000001 Rmu_sc0000697.1_g000015 Rmu_sc0002180.1_g000030 Rmu_sc0002509.1_g000011 Rmu_sc0002509.1_g000012 Rmu_sc0002509.1_g000020 Rmu_sc0002509.1_g000021 Rmu_sc0004459.1_g000003 Rmu_sc0004459.1_g000005 Rmu_sc0006767.1_g000007 Rmu_sc0014755.1_g000003 Rmu_sc0014755.1_g000004 Rmu_sc0036097.1_g000001
rosa_roxburghii Rroxscaffold_2G00089440 Rroxscaffold_3G00220040 Rroxscaffold_5G00334500 Rroxscaffold_5G00334530 Rroxscaffold_5G00334540 Rroxscaffold_5G00335080
rosa_rugosa Rorug02G0485300 Rorug03G0314100 Rorug03G0314200 Rorug03G0314500 Rorug03G0314500 Rorug03G0314600 Rorug03G0319800 Rorug03G0319900 Rorug03G0320000 Rorug06G0011400
rosa_samantha Rh4AG019700 Rh4AG019900 Rh4AG020200 Rh4AG023900 Rh4AG024000 Rh4AG024700 Rh4BG013900 Rh4BG014100 Rh4BG014200 Rh4BG014300 Rh4BG014400 Rh4BG014500 Rh4BG014600 Rh4BG014700 Rh4BG017100 Rh4BG017400 Rh4BG017500 Rh4BG017600 Rh4BG017700 Rh4BG089200 Rh4DG014500 Rh4DG014800 Rh4DG014900 Rh4DG015400 Rh4DG017900 Rh4DG018000 Rh6BG127900 Rh6CG126300 Rh6DG113400
rosa_wichuraiana Rw4G001270 Rw4G001320 Rw4G001350 Rw4G001700 Rw4G001710 Rw4G001720 Rw4G001760 Rw6G011430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1209
AccB7I CCANNNNNTGG 1 cut(s) 108
AccII CGCG 2 cut(s) 284, 405
AciI CCGC 6 cut(s) 85, 145, 276, 282, 403, 841
AclWI GGATC 2 cut(s) 46, 76
AcsI RAATTY 2 cut(s) 332, 398
AcuI CTGAAG 1 cut(s) 281
AfaI GTAC 1 cut(s) 1058
AfiI CCNNNNNNNGG 5 cut(s) 61, 108, 288, 576, 1094
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 5 cut(s) 91, 259, 592, 1167, 1231
AjnI CCWGG 2 cut(s) 1093, 1189
AleI CACNNNNGTG 1 cut(s) 503
AluBI AGCT 4 cut(s) 71, 182, 1227, 1325
AluI AGCT 4 cut(s) 71, 182, 1227, 1325
Alw26I GTCTC 4 cut(s) 103, 560, 749, 1310
AlwI GGATC 2 cut(s) 46, 76
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 3 cut(s) 237, 848, 851
ApoI RAATTY 2 cut(s) 332, 398
Asp700I GAANNNNTTC 2 cut(s) 587, 1263
AspLEI GCGC 3 cut(s) 407, 540, 704
AspS9I GGNCC 1 cut(s) 1102
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 5 cut(s) 592, 839, 1027, 1166, 1307
AsuII TTCGAA 2 cut(s) 482, 786
AvaII GGWCC 1 cut(s) 1102
BanII GRGCYC 1 cut(s) 42
BauI CACGAG 1 cut(s) 1134
BbsI GAAGAC 1 cut(s) 213
BbvI GCAGC 3 cut(s) 249, 835, 838
BccI CCATC 8 cut(s) 82, 224, 233, 720, 755, 1012, 1018, 1145
BceAI ACGGC 1 cut(s) 715
BciT130I CCWGG 2 cut(s) 1095, 1191
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 4 cut(s) 103, 560, 749, 1310
BfaI CTAG 2 cut(s) 609, 1268
BfoI RGCGCY 1 cut(s) 541
BfrI CTTAAG 1 cut(s) 44
BisI GCNGC 5 cut(s) 145, 238, 842, 849, 852
BlsI GCNGC 5 cut(s) 146, 239, 843, 850, 853
Bme1390I CCNGG 3 cut(s) 339, 1095, 1191
Bme18I GGWCC 1 cut(s) 1102
BmgT120I GGNCC 1 cut(s) 1102
BmiI GGNNCC 3 cut(s) 41, 66, 1104
BmrFI CCNGG 3 cut(s) 339, 1095, 1191
BoxI GACNNNNGTC 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 846
Bpu14I TTCGAA 2 cut(s) 482, 786
BpuMI CCSGG 1 cut(s) 339
BsaBI GATNNNNATC 1 cut(s) 1302
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 2 cut(s) 337, 529
BsaWI WCCGGW 2 cut(s) 569, 1075
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 5 cut(s) 61, 108, 288, 576, 1094
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 2 cut(s) 113, 863
Bse3DI GCAATG 1 cut(s) 313
Bse8I GATNNNNATC 1 cut(s) 1302
BseBI CCWGG 2 cut(s) 1095, 1191
BseDI CCNNGG 2 cut(s) 337, 529
BseGI GGATG 1 cut(s) 808
BseJI GATNNNNATC 1 cut(s) 1302
BseLI CCNNNNNNNGG 5 cut(s) 61, 108, 288, 576, 1094
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 3 cut(s) 192, 909, 1228
BseNI ACTGG 2 cut(s) 113, 863
BseRI GAGGAG 2 cut(s) 685, 849
BseXI GCAGC 3 cut(s) 249, 835, 838
BseYI CCCAGC 1 cut(s) 241
Bsh1236I CGCG 2 cut(s) 284, 405
BshFI GGCC 1 cut(s) 312
BsiSI CCGG 4 cut(s) 246, 339, 570, 1076
BslFI GGGAC 1 cut(s) 1046
BslI CCNNNNNNNGG 5 cut(s) 61, 108, 288, 576, 1094
BsmAI GTCTC 4 cut(s) 103, 560, 749, 1310
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 1046
BsmI GAATGC 1 cut(s) 654
BsnI GGCC 1 cut(s) 312
Bso31I GGTCTC 1 cut(s) 560
Bsp119I TTCGAA 2 cut(s) 482, 786
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 4 cut(s) 51, 81, 1066, 1171
BspACI CCGC 6 cut(s) 85, 145, 276, 282, 403, 841
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 3 cut(s) 191, 910, 1227
BspFNI CGCG 2 cut(s) 284, 405
BspLI GGNNCC 3 cut(s) 41, 66, 1104
BspPI GGATC 2 cut(s) 46, 76
BspT104I TTCGAA 2 cut(s) 482, 786
BspTI CTTAAG 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 2 cut(s) 113, 863
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 337, 529
BssMI GATC 4 cut(s) 51, 81, 1066, 1171
BssSI CACGAG 1 cut(s) 1134
Bst2BI CACGAG 1 cut(s) 1134
Bst2UI CCWGG 2 cut(s) 1095, 1191
Bst4CI ACNGT 3 cut(s) 22, 502, 778
Bst6I CTCTTC 2 cut(s) 196, 320
BstAFI CTTAAG 1 cut(s) 44
BstBI TTCGAA 2 cut(s) 482, 786
BstC8I GCNNGC 5 cut(s) 536, 629, 736, 740, 846
BstDEI CTNAG 5 cut(s) 178, 918, 1070, 1214, 1221
BstDSI CCRYGG 1 cut(s) 529
BstF5I GGATG 1 cut(s) 808
BstFNI CGCG 2 cut(s) 284, 405
BstH2I RGCGCY 1 cut(s) 541
BstHHI GCGC 3 cut(s) 407, 540, 704
BstKTI GATC 4 cut(s) 54, 84, 1069, 1174
BstMAI GTCTC 4 cut(s) 103, 560, 749, 1310
BstMBI GATC 4 cut(s) 51, 81, 1066, 1171
BstMWI GCNNNNNNNGC 4 cut(s) 46, 93, 275, 413
BstNI CCWGG 2 cut(s) 1095, 1191
BstPAI GACNNNNGTC 1 cut(s) 138
BstSCI CCNGG 3 cut(s) 337, 1093, 1189
BstUI CGCG 2 cut(s) 284, 405
BstV1I GCAGC 3 cut(s) 249, 835, 838
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BsuRI GGCC 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 529
BtsCI GGATG 1 cut(s) 808
BtsI GCAGTG 1 cut(s) 693
BtsIMutI CAGTG 3 cut(s) 106, 498, 693
Cac8I GCNNGC 5 cut(s) 536, 629, 736, 740, 846
CfoI GCGC 3 cut(s) 407, 540, 704
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 1 cut(s) 1102
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 1057
CviAII CATG 2 cut(s) 1061, 1312
CviQI GTAC 1 cut(s) 1057
DdeI CTNAG 5 cut(s) 178, 918, 1070, 1214, 1221
DpnI GATC 4 cut(s) 53, 83, 1068, 1173
DpnII GATC 4 cut(s) 51, 81, 1066, 1171
Eam1104I CTCTTC 2 cut(s) 196, 320
EarI CTCTTC 2 cut(s) 196, 320
EciI GGCGGA 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 560
Eco47I GGWCC 1 cut(s) 1102
Eco57I CTGAAG 1 cut(s) 281
EcoO109I RGGNCCY 1 cut(s) 1102
EcoRI GAATTC 1 cut(s) 332
EcoRII CCWGG 2 cut(s) 1093, 1189
EcoT22I ATGCAT 1 cut(s) 660
EcoT38I GRGCYC 1 cut(s) 42
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 2 cut(s) 1064, 1315
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 1 cut(s) 1046
FatI CATG 2 cut(s) 1060, 1311
FauNDI CATATG 1 cut(s) 1185
Fnu4HI GCNGC 5 cut(s) 145, 238, 842, 849, 852
FokI GGATG 1 cut(s) 815
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 5 cut(s) 145, 238, 842, 849, 852
FspBI CTAG 2 cut(s) 609, 1268
GlaI GCGC 3 cut(s) 406, 539, 703
GluI GCNGC 5 cut(s) 145, 238, 842, 849, 852
GsaI CCCAGC 1 cut(s) 245
GsuI CTGGAG 1 cut(s) 846
HaeII RGCGCY 1 cut(s) 541
HaeIII GGCC 1 cut(s) 312
HapII CCGG 4 cut(s) 246, 339, 570, 1076
HgaI GACGC 1 cut(s) 85
HhaI GCGC 3 cut(s) 407, 540, 704
Hin1II CATG 2 cut(s) 1064, 1315
Hin6I GCGC 3 cut(s) 405, 538, 702
HinP1I GCGC 3 cut(s) 405, 538, 702
HinfI GANTC 5 cut(s) 292, 783, 953, 1003, 1160
HpaII CCGG 4 cut(s) 246, 339, 570, 1076
HphI GGTGA 5 cut(s) 592, 839, 1027, 1166, 1307
Hpy188I TCNGA 6 cut(s) 81, 127, 158, 565, 619, 754
Hpy188III TCNNGA 4 cut(s) 518, 1134, 1216, 1231
Hpy99I CGWCG 2 cut(s) 565, 623
HpyAV CCTTC 6 cut(s) 29, 265, 427, 489, 624, 674
HpyCH4III ACNGT 3 cut(s) 22, 502, 778
HpyCH4V TGCA 5 cut(s) 306, 658, 696, 811, 1183
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 93, 275, 413
HpyF3I CTNAG 5 cut(s) 178, 918, 1070, 1214, 1221
Hsp92II CATG 2 cut(s) 1064, 1315
HspAI GCGC 3 cut(s) 405, 538, 702
Kzo9I GATC 4 cut(s) 51, 81, 1066, 1171
LmnI GCTCC 4 cut(s) 45, 76, 421, 672
Lsp1109I GCAGC 3 cut(s) 249, 835, 838
MaeI CTAG 2 cut(s) 609, 1268
MaeIII GTNAC 1 cut(s) 515
MalI GATC 4 cut(s) 53, 83, 1068, 1173
MboI GATC 4 cut(s) 51, 81, 1066, 1171
MflI RGATCY 1 cut(s) 51
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 6 cut(s) 271, 332, 398, 705, 797, 1007
MlyI GAGTC 2 cut(s) 962, 997
MmeI TCCRAC 5 cut(s) 150, 543, 642, 760, 873
Mph1103I ATGCAT 1 cut(s) 660
MroXI GAANNNNTTC 2 cut(s) 587, 1263
MseI TTAA 2 cut(s) 45, 1053
MslI CAYNNNNRTG 3 cut(s) 503, 663, 816
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 4 cut(s) 246, 339, 570, 1076
MspR9I CCNGG 3 cut(s) 339, 1095, 1191
Mva1269I GAATGC 1 cut(s) 654
MvaI CCWGG 2 cut(s) 1095, 1191
MvnI CGCG 2 cut(s) 284, 405
MwoI GCNNNNNNNGC 4 cut(s) 46, 93, 275, 413
NciI CCSGG 1 cut(s) 339
NdeI CATATG 1 cut(s) 1185
NdeII GATC 4 cut(s) 51, 81, 1066, 1171
NlaIII CATG 2 cut(s) 1064, 1315
NlaIV GGNNCC 3 cut(s) 41, 66, 1104
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 515
NsiI ATGCAT 1 cut(s) 660
NspV TTCGAA 2 cut(s) 482, 786
OliI CACNNNNGTG 1 cut(s) 503
PctI GAATGC 1 cut(s) 654
PdmI GAANNNNTTC 2 cut(s) 587, 1263
PfeI GAWTC 3 cut(s) 292, 783, 1160
PflMI CCANNNNNTGG 1 cut(s) 108
PfoI TCCNGGA 1 cut(s) 1093
PkrI GCNGC 5 cut(s) 146, 239, 843, 850, 853
PleI GAGTC 2 cut(s) 961, 997
PpsI GAGTC 2 cut(s) 961, 997
PpuMI RGGWCCY 1 cut(s) 1102
PshAI GACNNNNGTC 1 cut(s) 138
PsiI TTATAA 1 cut(s) 1209
Psp5II RGGWCCY 1 cut(s) 1102
Psp6I CCWGG 2 cut(s) 1093, 1189
PspFI CCCAGC 1 cut(s) 241
PspGI CCWGG 2 cut(s) 1093, 1189
PspN4I GGNNCC 3 cut(s) 41, 66, 1104
PspPI GGNCC 1 cut(s) 1102
PspPPI RGGWCCY 1 cut(s) 1102
PsuI RGATCY 1 cut(s) 51
RsaI GTAC 1 cut(s) 1058
RsaNI GTAC 1 cut(s) 1057
RseI CAYNNNNRTG 3 cut(s) 503, 663, 816
SaqAI TTAA 2 cut(s) 45, 1053
SatI GCNGC 5 cut(s) 145, 238, 842, 849, 852
Sau3AI GATC 4 cut(s) 51, 81, 1066, 1171
Sau96I GGNCC 1 cut(s) 1102
SchI GAGTC 2 cut(s) 962, 997
ScrFI CCNGG 3 cut(s) 339, 1095, 1191
SduI GDGCHC 1 cut(s) 42
SfuI TTCGAA 2 cut(s) 482, 786
SinI GGWCC 1 cut(s) 1102
SmiMI CAYNNNNRTG 3 cut(s) 503, 663, 816
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 6 cut(s) 271, 332, 398, 705, 797, 1007
SsiI CCGC 6 cut(s) 85, 145, 276, 282, 403, 841
SspMI CTAG 2 cut(s) 609, 1268
StyD4I CCNGG 3 cut(s) 337, 1093, 1189
TaaI ACNGT 3 cut(s) 22, 502, 778
TaqI TCGA 6 cut(s) 33, 330, 474, 482, 771, 786
TasI AATT 6 cut(s) 271, 332, 398, 705, 797, 1007
TauI GCSGC 2 cut(s) 147, 844
TfiI GAWTC 3 cut(s) 292, 783, 1160
Tru1I TTAA 2 cut(s) 45, 1053
Tru9I TTAA 2 cut(s) 45, 1053
TscAI CASTG 3 cut(s) 113, 505, 693
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 3 cut(s) 237, 848, 851
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 3 cut(s) 219, 698, 1299
TspGWI ACGGA 2 cut(s) 518, 879
TspRI CASTG 3 cut(s) 113, 505, 693
Van91I CCANNNNNTGG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 44
VpaK11BI GGWCC 1 cut(s) 1102
XapI RAATTY 2 cut(s) 332, 398
XmnI GAANNNNTTC 2 cut(s) 587, 1263
XspI CTAG 2 cut(s) 609, 1268
Zsp2I ATGCAT 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.