Rw4G001270

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000449
Physical Location & Seq
Reverse (-)
2317386 .. 2318723
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001270.1

Sequence Viewer

Length: 1167 bp
ATGTCATCGTCGGCAGAAACGGTAGCGAACATCGAAGGTCTCCTTAAGCAGATCCTCATATCGGTTCCGGCTCTATCTCTGATCCGCTTCAAGTGCGTCTCCAAGCACTGGCTCTCTCTCATTTCCGACCCCGACTTCTGTCGCCGCCACACTCTCCGAAATCCTAACCCCAAAATCTCCGCTTTCTTCTCTTCCCAAACCAATCAAGACACCACCGTCAACTCCATCACTCTCGGCAGCCCAGCCGGTAATCCCTTCAAAACTCTCGCCAATTCCGCCTCCGCGTTGAGGATTCTCCAGTCCTGCAATGGCCTCTTCCTCTGCTGTGTCGAATTCCCCGGAGAAGAAGAAGAAGAAAGAAACTATCCTCCTGTATATGTCGTCAATCCCACCACCAACAAATTCCGCGCTCTCTCTGCTCCAAGCCTTGCGAAGAAGGAGGAAATGTATATGTTTGTGAGGTATGGTTTGGCTTTCGACCCTTCGAAATCGCCTCATTACACTGTGGTGTGTGTCAGTCACGACATTCTCCGCGGCAAGCGCCACAGCATAGAGATATATTCGTCGGAGACGGGAACTTGGGTGAAGCGTTTCAAGGCCCCTTTCTTCCCCAGCCCTTCGGACGAGGGCAGGCACATCGATACGCGCAATATGCCTATGCATTTTGATGAGAGGAGCAGGGAAGGGGCAGTGTATTGCAACGGCGCAATTCATTGGATAAGAAACAGATGCGAAGCGAGCCTGCCATTCTGCTGGGTGAGAATTGGTGAGGTTGAAACAGTTGGGTTCGAAAGAAGTGAATTGGATGTGTTGCACTACTATGACATAGGTGAAGAAAGTCTACGGCTCGCTGCTGCTACTCCTCCAGTTCCTTTGGTTGTGAAAAACATTCCGTTGGGGAAAGAACAAAACTTTCCTACTGAGTTTCCCCATTTGATTGATAGATGCTTTGGCGAGTCAGGTGGTGGCCGTTTGTATTTGATTGAGGTTTTTGAGCATTGTAAGACTCAATTTGAGGTGATGGAGATGGAGAGGGACTACTCTGGATGGAATAAGAGCTTCAAGACATCTGTTGAGTTAGCCAATAGGGAATTTTTTCTAGCTTTGGATGATGATGAATATGAGGGTGATAACTATCCTTACATGGAGACTTTAGCTTGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

388

Amino Acids

44.11

Weight (kDa)

5.64

Isoelectric Point (pI)

61.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 14 - 48 9.3e-06 F-box domain
FBA_1 PF07734 97 - 196 1e-05 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12890 FvH4_4g01370 FvH4_4g01420 FvH4_4g01430 FvH4_4g01472 FvH4_4g01490 FvH4_6g31140 FvH4_6g31150 FvH4_6g31161 FvH4_6g43941 FvH4_6g43961
rosa_chinensis RchiOBHm_Chr4g0387951 RchiOBHm_Chr4g0388001 RchiOBHm_Chr4g0388011 RchiOBHm_Chr4g0388051 RchiOBHm_Chr4g0388631 RchiOBHm_Chr4g0388641 RchiOBHm_Chr4g0388661 RchiOBHm_Chr4g0388801
rosa_laevigata RLG00000010028 RLG00000010030 RLG00000010032 RLG00000010082 RLG00000010086 RLG00000010089 RLG00000010091 RLG00000014277
rosa_multiflora Rmu_co8138914.1_g000001 Rmu_sc0000697.1_g000015 Rmu_sc0002180.1_g000030 Rmu_sc0002509.1_g000011 Rmu_sc0002509.1_g000012 Rmu_sc0002509.1_g000020 Rmu_sc0002509.1_g000021 Rmu_sc0004459.1_g000003 Rmu_sc0004459.1_g000005 Rmu_sc0006767.1_g000007 Rmu_sc0014755.1_g000003 Rmu_sc0014755.1_g000004 Rmu_sc0036097.1_g000001
rosa_roxburghii Rroxscaffold_2G00089440 Rroxscaffold_3G00220040 Rroxscaffold_5G00334500 Rroxscaffold_5G00334530 Rroxscaffold_5G00334540 Rroxscaffold_5G00335080
rosa_rugosa Rorug02G0485300 Rorug03G0314100 Rorug03G0314200 Rorug03G0314500 Rorug03G0314500 Rorug03G0314600 Rorug03G0319800 Rorug03G0319900 Rorug03G0320000 Rorug06G0011400
rosa_samantha Rh4AG019700 Rh4AG019900 Rh4AG020200 Rh4AG023900 Rh4AG024000 Rh4AG024700 Rh4BG013900 Rh4BG014100 Rh4BG014200 Rh4BG014300 Rh4BG014400 Rh4BG014500 Rh4BG014600 Rh4BG014700 Rh4BG017100 Rh4BG017400 Rh4BG017500 Rh4BG017600 Rh4BG017700 Rh4BG089200 Rh4DG014500 Rh4DG014800 Rh4DG014900 Rh4DG015400 Rh4DG017900 Rh4DG018000 Rh6BG127900 Rh6CG126300 Rh6DG113400
rosa_wichuraiana Rw4G001270 Rw4G001320 Rw4G001350 Rw4G001700 Rw4G001710 Rw4G001720 Rw4G001760 Rw6G011430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AccB7I CCANNNNNTGG 1 cut(s) 108
AccI GTMKAC 1 cut(s) 841
AccII CGCG 4 cut(s) 284, 408, 534, 646
AciI CCGC 8 cut(s) 85, 145, 180, 276, 282, 406, 532, 534
AclWI GGATC 2 cut(s) 46, 76
AcoI YGGCCR 1 cut(s) 967
AcsI RAATTY 3 cut(s) 332, 401, 1091
AfiI CCNNNNNNNGG 3 cut(s) 61, 108, 288
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 5 cut(s) 91, 259, 595, 776, 1063
AleI CACNNNNGTG 1 cut(s) 506
AluBI AGCT 3 cut(s) 1059, 1103, 1157
AluI AGCT 3 cut(s) 1059, 1103, 1157
Alw26I GTCTC 4 cut(s) 44, 103, 563, 1142
AlwI GGATC 2 cut(s) 46, 76
AoxI GGCC 3 cut(s) 310, 597, 967
ApeKI GCWGC 3 cut(s) 237, 851, 854
ApoI RAATTY 3 cut(s) 332, 401, 1091
Asp700I GAANNNNTTC 2 cut(s) 590, 1095
AspLEI GCGC 4 cut(s) 410, 543, 648, 707
AspS9I GGNCC 1 cut(s) 598
AsuC2I CCSGG 1 cut(s) 339
AsuHPI GGTGA 6 cut(s) 595, 769, 779, 842, 1030, 1139
AsuII TTCGAA 2 cut(s) 485, 789
BarI GAAGNNNNNNTAC 2 cut(s) 825, 857
BbvI GCAGC 3 cut(s) 249, 838, 841
BccI CCATC 4 cut(s) 233, 1015, 1021, 1041
BceAI ACGGC 3 cut(s) 718, 860, 954
BcgI CGANNNNNNTGC 2 cut(s) 619, 653
BcnI CCSGG 1 cut(s) 339
BcoDI GTCTC 4 cut(s) 44, 103, 563, 1142
BfaI CTAG 1 cut(s) 1100
BfoI RGCGCY 1 cut(s) 544
BfrI CTTAAG 1 cut(s) 44
BisI GCNGC 5 cut(s) 145, 238, 535, 852, 855
BlsI GCNGC 5 cut(s) 146, 239, 536, 853, 856
Bme1390I CCNGG 1 cut(s) 339
BmgT120I GGNCC 1 cut(s) 598
BmiI GGNNCC 2 cut(s) 66, 600
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 2 cut(s) 719, 935
BoxI GACNNNNGTC 1 cut(s) 138
BpmI CTGGAG 2 cut(s) 281, 849
Bpu14I TTCGAA 2 cut(s) 485, 789
BpuMI CCSGG 1 cut(s) 339
Bsa29I ATCGAT 1 cut(s) 639
BsaBI GATNNNNATC 1 cut(s) 1134
BsaI GGTCTC 1 cut(s) 44
BsaJI CCNNGG 2 cut(s) 337, 532
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 108, 288
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 3 cut(s) 113, 298, 866
Bse3DI GCAATG 1 cut(s) 313
Bse8I GATNNNNATC 1 cut(s) 1134
BseCI ATCGAT 1 cut(s) 639
BseDI CCNNGG 2 cut(s) 337, 532
BseGI GGATG 3 cut(s) 811, 1052, 1114
BseJI GATNNNNATC 1 cut(s) 1134
BseLI CCNNNNNNNGG 3 cut(s) 61, 108, 288
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 1 cut(s) 912
BseNI ACTGG 3 cut(s) 113, 298, 866
BseRI GAGGAG 2 cut(s) 688, 852
BseXI GCAGC 3 cut(s) 249, 838, 841
BseYI CCCAGC 3 cut(s) 241, 611, 753
Bsh1236I CGCG 4 cut(s) 284, 408, 534, 646
BshFI GGCC 3 cut(s) 312, 599, 969
BshVI ATCGAT 1 cut(s) 639
BsiSI CCGG 3 cut(s) 68, 246, 339
BslFI GGGAC 1 cut(s) 1049
BslI CCNNNNNNNGG 3 cut(s) 61, 108, 288
BsmAI GTCTC 4 cut(s) 44, 103, 563, 1142
BsmBI CGTCTC 2 cut(s) 103, 563
BsmFI GGGAC 1 cut(s) 1049
BsnI GGCC 3 cut(s) 312, 599, 969
Bso31I GGTCTC 1 cut(s) 44
Bsp119I TTCGAA 2 cut(s) 485, 789
Bsp143I GATC 2 cut(s) 51, 81
BspACI CCGC 8 cut(s) 85, 145, 180, 276, 282, 406, 532, 534
BspANI GGCC 3 cut(s) 312, 599, 969
BspCNI CTCAG 1 cut(s) 913
BspDI ATCGAT 1 cut(s) 639
BspFNI CGCG 4 cut(s) 284, 408, 534, 646
BspLI GGNNCC 2 cut(s) 66, 600
BspPI GGATC 2 cut(s) 46, 76
BspT104I TTCGAA 2 cut(s) 485, 789
BspTI CTTAAG 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 3 cut(s) 113, 298, 866
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 337, 532
BssMI GATC 2 cut(s) 51, 81
Bst4CI ACNGT 4 cut(s) 22, 217, 505, 781
Bst6I CTCTTC 2 cut(s) 196, 320
BstAFI CTTAAG 1 cut(s) 44
BstBI TTCGAA 2 cut(s) 485, 789
BstC8I GCNNGC 5 cut(s) 539, 632, 739, 743, 849
BstDEI CTNAG 1 cut(s) 921
BstDSI CCRYGG 1 cut(s) 532
BstF5I GGATG 3 cut(s) 811, 1052, 1114
BstFNI CGCG 4 cut(s) 284, 408, 534, 646
BstH2I RGCGCY 1 cut(s) 544
BstHHI GCGC 4 cut(s) 410, 543, 648, 707
BstKTI GATC 2 cut(s) 54, 84
BstMAI GTCTC 4 cut(s) 44, 103, 563, 1142
BstMBI GATC 2 cut(s) 51, 81
BstMWI GCNNNNNNNGC 6 cut(s) 93, 275, 416, 540, 652, 738
BstPAI GACNNNNGTC 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 337
BstUI CGCG 4 cut(s) 284, 408, 534, 646
BstV1I GCAGC 3 cut(s) 249, 838, 841
BstX2I RGATCY 1 cut(s) 51
BstXI CCANNNNNNTGG 1 cut(s) 753
BstYI RGATCY 1 cut(s) 51
Bsu15I ATCGAT 1 cut(s) 639
BsuRI GGCC 3 cut(s) 312, 599, 969
BsuTUI ATCGAT 1 cut(s) 639
BtgI CCRYGG 1 cut(s) 532
BtsCI GGATG 3 cut(s) 811, 1052, 1114
BtsI GCAGTG 1 cut(s) 696
BtsIMutI CAGTG 3 cut(s) 106, 501, 696
Cac8I GCNNGC 5 cut(s) 539, 632, 739, 743, 849
CfoI GCGC 4 cut(s) 410, 543, 648, 707
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 1 cut(s) 598
Cfr42I CCGCGG 1 cut(s) 535
ClaI ATCGAT 1 cut(s) 639
CseI GACGC 1 cut(s) 85
CviAII CATG 1 cut(s) 1144
DdeI CTNAG 1 cut(s) 921
DpnI GATC 2 cut(s) 53, 83
DpnII GATC 2 cut(s) 51, 81
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 1 cut(s) 967
Eam1104I CTCTTC 2 cut(s) 196, 320
EarI CTCTTC 2 cut(s) 196, 320
EciI GGCGGA 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 332
EcoT22I ATGCAT 1 cut(s) 663
Esp3I CGTCTC 2 cut(s) 103, 563
FaeI CATG 1 cut(s) 1147
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 1 cut(s) 1049
FatI CATG 1 cut(s) 1143
FblI GTMKAC 1 cut(s) 841
Fnu4HI GCNGC 5 cut(s) 145, 238, 535, 852, 855
FokI GGATG 3 cut(s) 818, 1059, 1121
Fsp4HI GCNGC 5 cut(s) 145, 238, 535, 852, 855
FspBI CTAG 1 cut(s) 1100
GlaI GCGC 4 cut(s) 409, 542, 647, 706
GluI GCNGC 5 cut(s) 145, 238, 535, 852, 855
GsaI CCCAGC 3 cut(s) 245, 615, 757
GsuI CTGGAG 2 cut(s) 281, 849
HaeII RGCGCY 1 cut(s) 544
HaeIII GGCC 3 cut(s) 312, 599, 969
HapII CCGG 3 cut(s) 68, 246, 339
HgaI GACGC 1 cut(s) 85
HhaI GCGC 4 cut(s) 410, 543, 648, 707
Hin1II CATG 1 cut(s) 1147
Hin6I GCGC 4 cut(s) 408, 541, 646, 705
HinP1I GCGC 4 cut(s) 408, 541, 646, 705
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 3 cut(s) 292, 956, 1006
HpaII CCGG 3 cut(s) 68, 246, 339
HphI GGTGA 6 cut(s) 595, 769, 779, 842, 1030, 1139
Hpy166II GTNNAC 2 cut(s) 220, 842
Hpy188I TCNGA 5 cut(s) 81, 127, 158, 568, 622
Hpy188III TCNNGA 4 cut(s) 206, 521, 1044, 1063
Hpy8I GTNNAC 2 cut(s) 220, 842
Hpy99I CGWCG 2 cut(s) 13, 568
HpyAV CCTTC 6 cut(s) 29, 265, 430, 492, 627, 677
HpyCH4III ACNGT 4 cut(s) 22, 217, 505, 781
HpyCH4V TGCA 4 cut(s) 306, 661, 699, 814
HpyF10VI GCNNNNNNNGC 6 cut(s) 93, 275, 416, 540, 652, 738
HpyF3I CTNAG 1 cut(s) 921
Hsp92II CATG 1 cut(s) 1147
HspAI GCGC 4 cut(s) 408, 541, 646, 705
KspI CCGCGG 1 cut(s) 535
Kzo9I GATC 2 cut(s) 51, 81
LmnI GCTCC 2 cut(s) 424, 675
Lsp1109I GCAGC 3 cut(s) 249, 838, 841
LweI GCATC 2 cut(s) 719, 935
MaeI CTAG 1 cut(s) 1100
MaeIII GTNAC 1 cut(s) 518
MalI GATC 2 cut(s) 53, 83
MboI GATC 2 cut(s) 51, 81
MflI RGATCY 1 cut(s) 51
MluCI AATT 8 cut(s) 271, 332, 401, 708, 762, 800, 1010, 1091
MlyI GAGTC 2 cut(s) 965, 1000
MmeI TCCRAC 2 cut(s) 150, 546
Mph1103I ATGCAT 1 cut(s) 663
MroXI GAANNNNTTC 2 cut(s) 590, 1095
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 3 cut(s) 506, 666, 819
MspA1I CMGCKG 1 cut(s) 534
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 3 cut(s) 68, 246, 339
MspR9I CCNGG 1 cut(s) 339
MvnI CGCG 4 cut(s) 284, 408, 534, 646
MwoI GCNNNNNNNGC 6 cut(s) 93, 275, 416, 540, 652, 738
NciI CCSGG 1 cut(s) 339
NdeII GATC 2 cut(s) 51, 81
NlaIII CATG 1 cut(s) 1147
NlaIV GGNNCC 2 cut(s) 66, 600
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 518
NsiI ATGCAT 1 cut(s) 663
NspV TTCGAA 2 cut(s) 485, 789
OliI CACNNNNGTG 1 cut(s) 506
PdmI GAANNNNTTC 2 cut(s) 590, 1095
PfeI GAWTC 1 cut(s) 292
PflMI CCANNNNNTGG 1 cut(s) 108
PkrI GCNGC 5 cut(s) 146, 239, 536, 853, 856
PleI GAGTC 2 cut(s) 964, 1000
PpsI GAGTC 2 cut(s) 964, 1000
PshAI GACNNNNGTC 1 cut(s) 138
PspFI CCCAGC 3 cut(s) 241, 611, 753
PspN4I GGNNCC 2 cut(s) 66, 600
PspPI GGNCC 1 cut(s) 598
PsuI RGATCY 1 cut(s) 51
RseI CAYNNNNRTG 3 cut(s) 506, 666, 819
SacII CCGCGG 1 cut(s) 535
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 5 cut(s) 145, 238, 535, 852, 855
Sau3AI GATC 2 cut(s) 51, 81
Sau96I GGNCC 1 cut(s) 598
SchI GAGTC 2 cut(s) 965, 1000
ScrFI CCNGG 1 cut(s) 339
SfaNI GCATC 2 cut(s) 719, 935
Sfr303I CCGCGG 1 cut(s) 535
SfuI TTCGAA 2 cut(s) 485, 789
SgrBI CCGCGG 1 cut(s) 535
SmiMI CAYNNNNRTG 3 cut(s) 506, 666, 819
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 8 cut(s) 271, 332, 401, 708, 762, 800, 1010, 1091
SsiI CCGC 8 cut(s) 85, 145, 180, 276, 282, 406, 532, 534
SspMI CTAG 1 cut(s) 1100
StyD4I CCNGG 1 cut(s) 337
TaaI ACNGT 4 cut(s) 22, 217, 505, 781
TaqI TCGA 6 cut(s) 33, 330, 477, 485, 639, 789
TasI AATT 8 cut(s) 271, 332, 401, 708, 762, 800, 1010, 1091
TauI GCSGC 2 cut(s) 147, 537
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 3 cut(s) 113, 508, 696
TseFI GTSAC 1 cut(s) 518
TseI GCWGC 3 cut(s) 237, 851, 854
Tsp45I GTSAC 1 cut(s) 518
TspDTI ATGAA 2 cut(s) 701, 1131
TspGWI ACGGA 1 cut(s) 882
TspRI CASTG 3 cut(s) 113, 508, 696
Van91I CCANNNNNTGG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 44
XapI RAATTY 3 cut(s) 332, 401, 1091
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 841
XmnI GAANNNNTTC 2 cut(s) 590, 1095
XspI CTAG 1 cut(s) 1100
Zsp2I ATGCAT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.