Rh4AG024000

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000449
Physical Location & Seq
Forward (+)
4706022 .. 4707884
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG024000.1

Sequence Viewer

Length: 1209 bp
ATGGCGCAAACAAAAGAAAGGAGAATTCGATGCGATTCCTCATCAGCAGCAGAAACGGTGGCCGATGTCGAGGAGCTTCTCACAAAGATCCTTGTGTCGGTGCCACCTCGATCTCTGGTTCGTTTCAAGTGCGTCTCCAAGCACTGGCTCACTCTCATCTCCGACCCCGGATTCTTTCGCCGCCAGACACATCAAAAGTCGAAGATCTCCGGTTTCTTTTCTAGCAAAACCGAAGACGAGTTCTTCAAGTCCATTGCTCTTCGTGACCGTGAAATCCCATCTGGGAATCCCTTCAAAACTATTAATGATTCCTTCGCTGATGGATCCAAGTTGAGGATTCTTCAGTCCAGCAATGGCCTCTTCCTCTGCCATATTCCTATAGTAGACGTGCTTGAAGAAGGAAAACATCATCCCTTGTATGTTGTCAATCCAACAACCAACCAATTCCGGGCTCTTTCTTGTCCAAGCTCTGAGGCCTGGGACCCGTTCATGTTTGTACGTTATGCTTTGGCTTTTAATCCTTCCAAGTCGCCTCATTACAAGGTGATCTGCGTAAATAACTTCCCCTATTGTTATGATAGAGGCCACCACGAAATAGACATATATTCATCTGAGACCGGAGAGTGGAAGCGTCTTGAGGTTCCTTTCTTCCCAAGCCCCTCTGACGTGGGCAGACACCATGATTTCGTGCAGAAGGCCATGCACTTTGACTACAGGAGCAGGGAAGGCGCCGTATACTGCAACGGGGCAGTTCATTGGATAAGAGACAGAAGGGAAGCAATTTTTCCACTCCGCTTTTTTGGGGATGGTAGGTCTGAATTGGTAAGAAACGAAACTGATGTGCTGCACTACTTTGACATAGGCGAAGAACGTTTGGGACTTGTGTCTGCTACCCCTCCTGTCCCCTTGGTTGTCAAAAACATTCCGTTGGATTTTGGTTCATGTCCCACTGAATGGCCGAGATTGGCTCAAAGATATTTTGGGGAGTCCGGTGGCCATTTGTATTTGATTGAGACTTATCAGCATTGTAAGACCCAATTTGATGTGATGGAGATGGAGAGGGACTACTCTGGCTGGTTTGTCAATAAGAAGGCTTTCACTGGTCAGACTGTAATCATAGTGAAAAAGGATGAGAATGCTAACAATCCTCTCCCATTTTCTAACTCCCATTTCAGATATGTTTGCCGTTTGCCCTTTGGAACAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

402

Amino Acids

46.34

Weight (kDa)

8.0

Isoelectric Point (pI)

49.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 24 - 60 1.1e-06 F-box domain
FBA_1 PF07734 111 - 296 5.8e-09 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12890 FvH4_4g01370 FvH4_4g01420 FvH4_4g01430 FvH4_4g01472 FvH4_4g01490 FvH4_6g31140 FvH4_6g31150 FvH4_6g31161 FvH4_6g43941 FvH4_6g43961
rosa_chinensis RchiOBHm_Chr4g0387951 RchiOBHm_Chr4g0388001 RchiOBHm_Chr4g0388011 RchiOBHm_Chr4g0388051 RchiOBHm_Chr4g0388631 RchiOBHm_Chr4g0388641 RchiOBHm_Chr4g0388661 RchiOBHm_Chr4g0388801
rosa_laevigata RLG00000010028 RLG00000010030 RLG00000010032 RLG00000010082 RLG00000010086 RLG00000010089 RLG00000010091 RLG00000014277
rosa_multiflora Rmu_co8138914.1_g000001 Rmu_sc0000697.1_g000015 Rmu_sc0002180.1_g000030 Rmu_sc0002509.1_g000011 Rmu_sc0002509.1_g000012 Rmu_sc0002509.1_g000020 Rmu_sc0002509.1_g000021 Rmu_sc0004459.1_g000003 Rmu_sc0004459.1_g000005 Rmu_sc0006767.1_g000007 Rmu_sc0014755.1_g000003 Rmu_sc0014755.1_g000004 Rmu_sc0036097.1_g000001
rosa_roxburghii Rroxscaffold_2G00089440 Rroxscaffold_3G00220040 Rroxscaffold_5G00334500 Rroxscaffold_5G00334530 Rroxscaffold_5G00334540 Rroxscaffold_5G00335080
rosa_rugosa Rorug02G0485300 Rorug03G0314100 Rorug03G0314200 Rorug03G0314500 Rorug03G0314500 Rorug03G0314600 Rorug03G0319800 Rorug03G0319900 Rorug03G0320000 Rorug06G0011400
rosa_samantha Rh4AG019700 Rh4AG019900 Rh4AG020200 Rh4AG023900 Rh4AG024000 Rh4AG024700 Rh4BG013900 Rh4BG014100 Rh4BG014200 Rh4BG014300 Rh4BG014400 Rh4BG014500 Rh4BG014600 Rh4BG014700 Rh4BG017100 Rh4BG017400 Rh4BG017500 Rh4BG017600 Rh4BG017700 Rh4BG089200 Rh4DG014500 Rh4DG014800 Rh4DG014900 Rh4DG015400 Rh4DG017900 Rh4DG018000 Rh6BG127900 Rh6CG126300 Rh6DG113400
rosa_wichuraiana Rw4G001270 Rw4G001320 Rw4G001350 Rw4G001700 Rw4G001710 Rw4G001720 Rw4G001760 Rw6G011430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 100, 728
AccB7I CCANNNNNTGG 2 cut(s) 144, 954
AccI GTMKAC 2 cut(s) 384, 735
AciI CCGC 2 cut(s) 181, 793
AclI AACGTT 1 cut(s) 871
AclWI GGATC 3 cut(s) 82, 318, 331
AcoI YGGCCR 3 cut(s) 60, 956, 994
AcsI RAATTY 1 cut(s) 24
AcuI CTGAAG 1 cut(s) 326
AcyI GRCGYC 1 cut(s) 729
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 6 cut(s) 97, 144, 333, 448, 624, 954
AgsI TTSAA 4 cut(s) 127, 247, 295, 395
AjiI CACGTC 2 cut(s) 388, 667
AjnI CCWGG 1 cut(s) 476
AluBI AGCT 2 cut(s) 76, 468
AluI AGCT 2 cut(s) 76, 468
Alw26I GTCTC 4 cut(s) 139, 608, 759, 1007
AlwI GGATC 3 cut(s) 82, 318, 331
AoxI GGCC 7 cut(s) 60, 355, 474, 583, 696, 956, 994
ApeKI GCWGC 2 cut(s) 47, 844
ApoI RAATTY 1 cut(s) 24
AseI ATTAAT 1 cut(s) 303
Asp700I GAANNNNTTC 2 cut(s) 290, 1094
AspLEI GCGC 2 cut(s) 7, 731
AspS9I GGNCC 1 cut(s) 481
AsuC2I CCSGG 2 cut(s) 168, 449
AsuHPI GGTGA 1 cut(s) 556
AvaII GGWCC 1 cut(s) 481
BalI TGGCCA 1 cut(s) 996
BamHI GGATCC 1 cut(s) 323
BanI GGYRCC 2 cut(s) 100, 728
BanII GRGCYC 1 cut(s) 454
BbsI GAAGAC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 59, 831
BccI CCATC 5 cut(s) 286, 314, 800, 1042, 1048
BceAI ACGGC 2 cut(s) 716, 1170
BciT130I CCWGG 1 cut(s) 478
BcnI CCSGG 2 cut(s) 168, 449
BcoDI GTCTC 4 cut(s) 139, 608, 759, 1007
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 2 cut(s) 378, 712
BfoI RGCGCY 1 cut(s) 732
BglII AGATCT 1 cut(s) 204
BisI GCNGC 3 cut(s) 48, 181, 845
BlsI GCNGC 3 cut(s) 49, 182, 846
Bme1390I CCNGG 3 cut(s) 168, 449, 478
Bme18I GGWCC 1 cut(s) 481
BmgBI CACGTC 2 cut(s) 388, 667
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 6 cut(s) 102, 325, 482, 483, 642, 730
BmrFI CCNGG 3 cut(s) 168, 449, 478
BmsI GCATC 1 cut(s) 20
BoxI GACNNNNGTC 1 cut(s) 883
BpiI GAAGAC 1 cut(s) 240
BplI GAGNNNNNCTC 2 cut(s) 952, 984
BpuEI CTTGAG 1 cut(s) 656
BpuMI CCSGG 2 cut(s) 168, 449
BsaHI GRCGYC 1 cut(s) 729
BsaI GGTCTC 1 cut(s) 608
BsaJI CCNNGG 3 cut(s) 166, 477, 906
BsaWI WCCGGW 3 cut(s) 209, 617, 989
Bsc4I CCNNNNNNNGG 6 cut(s) 97, 144, 333, 448, 624, 954
Bse1I ACTGG 2 cut(s) 149, 1105
Bse3DI GCAATG 2 cut(s) 252, 358
BseBI CCWGG 1 cut(s) 478
BseDI CCNNGG 3 cut(s) 166, 477, 906
BseGI GGATG 3 cut(s) 409, 811, 1135
BseLI CCNNNNNNNGG 6 cut(s) 97, 144, 333, 448, 624, 954
BseMI GCAATG 2 cut(s) 252, 358
BseMII CTCAG 2 cut(s) 462, 603
BseNI ACTGG 2 cut(s) 149, 1105
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 2 cut(s) 59, 831
BsgI GTGCAG 2 cut(s) 710, 830
BshFI GGCC 7 cut(s) 62, 357, 476, 585, 698, 958, 996
BshNI GGYRCC 2 cut(s) 100, 728
BsiSI CCGG 5 cut(s) 168, 210, 448, 618, 990
BslFI GGGAC 5 cut(s) 494, 887, 891, 930, 1076
BslI CCNNNNNNNGG 6 cut(s) 97, 144, 333, 448, 624, 954
BsmAI GTCTC 4 cut(s) 139, 608, 759, 1007
BsmBI CGTCTC 1 cut(s) 139
BsmFI GGGAC 5 cut(s) 494, 887, 891, 930, 1076
BsmI GAATGC 1 cut(s) 1141
BsnI GGCC 7 cut(s) 62, 357, 476, 585, 698, 958, 996
Bso31I GGTCTC 1 cut(s) 608
Bsp1286I GDGCHC 1 cut(s) 454
Bsp143I GATC 5 cut(s) 87, 110, 204, 323, 546
BspACI CCGC 2 cut(s) 181, 793
BspANI GGCC 7 cut(s) 62, 357, 476, 585, 698, 958, 996
BspCNI CTCAG 2 cut(s) 463, 604
BspLI GGNNCC 6 cut(s) 102, 325, 482, 483, 642, 730
BspPI GGATC 3 cut(s) 82, 318, 331
BspQI GCTCTTC 1 cut(s) 264
BspT107I GGYRCC 2 cut(s) 100, 728
BspTNI GGTCTC 1 cut(s) 608
BsrDI GCAATG 2 cut(s) 252, 358
BsrI ACTGG 2 cut(s) 149, 1105
BssECI CCNNGG 3 cut(s) 166, 477, 906
BssMI GATC 5 cut(s) 87, 110, 204, 323, 546
BssNAI GTATAC 1 cut(s) 736
BssNI GRCGYC 1 cut(s) 729
BssT1I CCWWGG 1 cut(s) 906
Bst1107I GTATAC 1 cut(s) 736
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 3 cut(s) 58, 269, 1111
Bst6I CTCTTC 2 cut(s) 264, 365
BstACI GRCGYC 1 cut(s) 729
BstDEI CTNAG 2 cut(s) 471, 612
BstF5I GGATG 3 cut(s) 409, 811, 1135
BstH2I RGCGCY 1 cut(s) 732
BstHHI GCGC 2 cut(s) 7, 731
BstKTI GATC 5 cut(s) 90, 113, 207, 326, 549
BstMAI GTCTC 4 cut(s) 139, 608, 759, 1007
BstMBI GATC 5 cut(s) 87, 110, 204, 323, 546
BstMWI GCNNNNNNNGC 1 cut(s) 726
BstNI CCWGG 1 cut(s) 478
BstPAI GACNNNNGTC 1 cut(s) 883
BstSCI CCNGG 3 cut(s) 166, 447, 476
BstSFI CTRYAG 2 cut(s) 378, 712
BstV1I GCAGC 2 cut(s) 59, 831
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 3 cut(s) 87, 204, 323
BstYI RGATCY 3 cut(s) 87, 204, 323
BstZ17I GTATAC 1 cut(s) 736
BsuRI GGCC 7 cut(s) 62, 357, 476, 585, 698, 958, 996
BtrI CACGTC 2 cut(s) 388, 667
BtsCI GGATG 3 cut(s) 409, 811, 1135
BtsIMutI CAGTG 3 cut(s) 142, 948, 1098
CfoI GCGC 2 cut(s) 7, 731
Cfr13I GGNCC 1 cut(s) 481
CseI GACGC 2 cut(s) 121, 620
Csp6I GTAC 1 cut(s) 497
CviAII CATG 4 cut(s) 490, 680, 700, 942
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 2 cut(s) 471, 612
DinI GGCGCC 1 cut(s) 730
DpnI GATC 5 cut(s) 89, 112, 206, 325, 548
DpnII GATC 5 cut(s) 87, 110, 204, 323, 546
EaeI YGGCCR 3 cut(s) 60, 956, 994
Eam1104I CTCTTC 2 cut(s) 264, 365
EarI CTCTTC 2 cut(s) 264, 365
Eco130I CCWWGG 1 cut(s) 906
Eco147I AGGCCT 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 454
Eco31I GGTCTC 1 cut(s) 608
Eco47I GGWCC 1 cut(s) 481
Eco57I CTGAAG 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 481
EcoRI GAATTC 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 906
EcoT38I GRGCYC 1 cut(s) 454
EgeI GGCGCC 1 cut(s) 730
EheI GGCGCC 1 cut(s) 730
ErhI CCWWGG 1 cut(s) 906
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 4 cut(s) 493, 683, 703, 945
FaqI GGGAC 5 cut(s) 494, 887, 891, 930, 1076
FatI CATG 4 cut(s) 489, 679, 699, 941
FblI GTMKAC 2 cut(s) 384, 735
Fnu4HI GCNGC 3 cut(s) 48, 181, 845
FokI GGATG 3 cut(s) 396, 818, 1142
FriOI GRGCYC 1 cut(s) 454
Fsp4HI GCNGC 3 cut(s) 48, 181, 845
FspBI CTAG 1 cut(s) 222
GlaI GCGC 2 cut(s) 6, 730
GluI GCNGC 3 cut(s) 48, 181, 845
HaeII RGCGCY 1 cut(s) 732
HaeIII GGCC 7 cut(s) 62, 357, 476, 585, 698, 958, 996
HapII CCGG 5 cut(s) 168, 210, 448, 618, 990
HgaI GACGC 2 cut(s) 121, 620
HhaI GCGC 2 cut(s) 7, 731
Hin1I GRCGYC 1 cut(s) 729
Hin1II CATG 4 cut(s) 493, 683, 703, 945
Hin6I GCGC 2 cut(s) 5, 729
HinP1I GCGC 2 cut(s) 5, 729
HinfI GANTC 6 cut(s) 35, 171, 286, 308, 337, 986
HpaII CCGG 5 cut(s) 168, 210, 448, 618, 990
HphI GGTGA 1 cut(s) 556
Hpy166II GTNNAC 2 cut(s) 385, 736
Hpy188I TCNGA 7 cut(s) 163, 472, 613, 664, 817, 1107, 1175
Hpy188III TCNNGA 2 cut(s) 263, 635
Hpy8I GTNNAC 2 cut(s) 385, 736
HpyAV CCTTC 8 cut(s) 301, 322, 392, 531, 688, 719, 765, 1084
HpyCH4III ACNGT 3 cut(s) 58, 269, 1111
HpyCH4IV ACGT 4 cut(s) 387, 499, 666, 871
HpyCH4V TGCA 4 cut(s) 691, 703, 741, 847
HpyF10VI GCNNNNNNNGC 1 cut(s) 726
HpyF3I CTNAG 2 cut(s) 471, 612
HpySE526I ACGT 4 cut(s) 387, 499, 666, 871
Hsp92I GRCGYC 1 cut(s) 729
Hsp92II CATG 4 cut(s) 493, 683, 703, 945
HspAI GCGC 2 cut(s) 5, 729
KasI GGCGCC 1 cut(s) 728
KflI GGGWCCC 1 cut(s) 481
Kzo9I GATC 5 cut(s) 87, 110, 204, 323, 546
LguI GCTCTTC 1 cut(s) 264
LmnI GCTCC 2 cut(s) 73, 717
Lsp1109I GCAGC 2 cut(s) 59, 831
LweI GCATC 1 cut(s) 20
MaeI CTAG 1 cut(s) 222
MaeII ACGT 4 cut(s) 387, 499, 666, 871
MaeIII GTNAC 1 cut(s) 263
MalI GATC 5 cut(s) 89, 112, 206, 325, 548
MboI GATC 5 cut(s) 87, 110, 204, 323, 546
MboII GAAGA 9 cut(s) 214, 235, 245, 251, 332, 352, 407, 640, 878
MflI RGATCY 3 cut(s) 87, 204, 323
MhlI GDGCHC 1 cut(s) 454
MlsI TGGCCA 1 cut(s) 996
MluCI AATT 6 cut(s) 24, 443, 780, 818, 1037, 1203
MluNI TGGCCA 1 cut(s) 996
Mly113I GGCGCC 1 cut(s) 729
MlyI GAGTC 1 cut(s) 995
MmeI TCCRAC 3 cut(s) 186, 455, 909
Mox20I TGGCCA 1 cut(s) 996
MroXI GAANNNNTTC 2 cut(s) 290, 1094
MscI TGGCCA 1 cut(s) 996
MseI TTAA 2 cut(s) 303, 516
Msp20I TGGCCA 1 cut(s) 996
MspI CCGG 5 cut(s) 168, 210, 448, 618, 990
MspR9I CCNGG 3 cut(s) 168, 449, 478
Mva1269I GAATGC 1 cut(s) 1141
MvaI CCWGG 1 cut(s) 478
MwoI GCNNNNNNNGC 1 cut(s) 726
NarI GGCGCC 1 cut(s) 729
NciI CCSGG 2 cut(s) 168, 449
NdeII GATC 5 cut(s) 87, 110, 204, 323, 546
NlaIII CATG 4 cut(s) 493, 683, 703, 945
NlaIV GGNNCC 6 cut(s) 102, 325, 482, 483, 642, 730
NmeAIII GCCGAG 1 cut(s) 984
NmuCI GTSAC 1 cut(s) 263
PceI AGGCCT 1 cut(s) 476
PciSI GCTCTTC 1 cut(s) 264
PctI GAATGC 1 cut(s) 1141
PdmI GAANNNNTTC 2 cut(s) 290, 1094
PfeI GAWTC 5 cut(s) 35, 171, 286, 308, 337
PflMI CCANNNNNTGG 2 cut(s) 144, 954
PkrI GCNGC 3 cut(s) 49, 182, 846
PleI GAGTC 1 cut(s) 994
PluTI GGCGCC 1 cut(s) 732
PpsI GAGTC 1 cut(s) 994
PpuMI RGGWCCY 1 cut(s) 481
PshAI GACNNNNGTC 1 cut(s) 883
PshBI ATTAAT 1 cut(s) 303
Psp1406I AACGTT 1 cut(s) 871
Psp5II RGGWCCY 1 cut(s) 481
Psp6I CCWGG 1 cut(s) 476
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 6 cut(s) 102, 325, 482, 483, 642, 730
PspPI GGNCC 1 cut(s) 481
PspPPI RGGWCCY 1 cut(s) 481
PsuI RGATCY 3 cut(s) 87, 204, 323
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
SapI GCTCTTC 1 cut(s) 264
SaqAI TTAA 2 cut(s) 303, 516
SatI GCNGC 3 cut(s) 48, 181, 845
Sau3AI GATC 5 cut(s) 87, 110, 204, 323, 546
Sau96I GGNCC 1 cut(s) 481
SchI GAGTC 1 cut(s) 995
ScrFI CCNGG 3 cut(s) 168, 449, 478
SduI GDGCHC 1 cut(s) 454
SfaNI GCATC 1 cut(s) 20
SfcI CTRYAG 2 cut(s) 378, 712
SfoI GGCGCC 1 cut(s) 730
SinI GGWCC 1 cut(s) 481
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
Sse9I AATT 6 cut(s) 24, 443, 780, 818, 1037, 1203
SseBI AGGCCT 1 cut(s) 476
SsiI CCGC 2 cut(s) 181, 793
SspDI GGCGCC 1 cut(s) 728
SspMI CTAG 1 cut(s) 222
StuI AGGCCT 1 cut(s) 476
StyD4I CCNGG 3 cut(s) 166, 447, 476
StyI CCWWGG 1 cut(s) 906
TaaI ACNGT 3 cut(s) 58, 269, 1111
TaiI ACGT 4 cut(s) 390, 502, 669, 874
TaqI TCGA 4 cut(s) 28, 69, 109, 200
TasI AATT 6 cut(s) 24, 443, 780, 818, 1037, 1203
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 5 cut(s) 35, 171, 286, 308, 337
Tru1I TTAA 2 cut(s) 303, 516
Tru9I TTAA 2 cut(s) 303, 516
TscAI CASTG 3 cut(s) 149, 955, 1105
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 2 cut(s) 47, 844
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 4 cut(s) 478, 597, 743, 930
TspGWI ACGGA 1 cut(s) 915
TspRI CASTG 3 cut(s) 149, 955, 1105
Van91I CCANNNNNTGG 2 cut(s) 144, 954
VpaK11BI GGWCC 1 cut(s) 481
VspI ATTAAT 1 cut(s) 303
XapI RAATTY 1 cut(s) 24
XmiI GTMKAC 2 cut(s) 384, 735
XmnI GAANNNNTTC 2 cut(s) 290, 1094
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.