Rh4BG014300

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000449
Physical Location & Seq
Reverse (-)
2012572 .. 2016792
4221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG014300.1

Sequence Viewer

Length: 1221 bp
ATGTCATCTTCAGCAGAAACCGTCGCGAATATCGAAGACCTGCTTAAGCAGATCCTTATATGGGTTCCAGCTCTATCTCTGATCCGTTTCAAGTGCGTCTCCAAGCAATGGCTCTCTCTCATCTCCGACCCCGAGTTCCGTCGCCGCCACACCCTCCGAAACCCTAACTCCAAAATCTCAGCTTTCTTCTCTAGCAAAATCCAAGACGAGTACTTCAAGTCCATCCTTCATGGTAACCATGAAATCCCACCTGGGTGGAATCCCTTCAAAACCCTTAACAATTCCGATTCCGTCCCTGATGAATCCAAGTTGAGGATTCTTCAGTCCTGCAATGGCCTCTTTCTCTGCCATATTCCTAGATCTGGACTTGAAACAAAAAATCATCCCGTGTATGTTGTCAATCCCACAACCAACGAATTCCGGGCTCTTTCTTTTCCAGTTGTCAGACATAGTAGTGATTCTTTATTTGTGCGCTATGCTTTGGCTTTTGATCCTTCCATATCGCCTCATTACAAGGTGGTCTGCGTGAATAACTTCACCTTTTATTATAATAGGGGGAAGCACAAAATAGACATATATTCATCTAAGACCGGAGAGTGGAAGCATCTCGATACTCCTTTCTTCCCCAGCCCCTCCGACGAGGGCAGGCACCCTCATTTCATGAACAAGGCCATGCACTTTGACTGCAGGAGCAGAGAAGGCGCCATATACTGCAACGGCGCAGTTCATTGGATAAGAGACATAGGGGAAGCAAGCTTGCCATTCTACTCTTTCCCAGACGGTAAATTCATAAGAAACGAAGCTGATGTGCTGCACTACTTCGACATAGGCCAAGAGCGTTTTCTGGTTGCTTCAGCTACCCCTCCTCTCCCCCTGGTTGCCAAGAACTTTCCACCGTTATTGACTCAGAGATATTTTGGGGAGTGTGGTGGCTGTTTGTACTTGATTGAGATTTATCAGCATTGCAACACTCAATATTTTGAGGTCATGGAGATGGAGACGGACTACTCTGGCTGGTTTTTCAAGTATCGACACTATCGTGTTGATCTTAATCCGATAGTTGCAGCTCTTCCCGGACAGGATTGGAATGCTTTTGTTGTCTTGTGCCTTTCTGGGGAGGAGGAAACCTCCCAAGAAATAGATGACAAGGTTGAGGATTCTTCAACAGATCTTTTGTTGCACATGCCTGATCGAGCATTATTGACTCTGAATCCCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

406

Amino Acids

46.75

Weight (kDa)

6.18

Isoelectric Point (pI)

54.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 12 - 48 4.3e-07 F-box domain
FBA_1 PF07734 93 - 282 2.2e-11 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12890 FvH4_4g01370 FvH4_4g01420 FvH4_4g01430 FvH4_4g01472 FvH4_4g01490 FvH4_6g31140 FvH4_6g31150 FvH4_6g31161 FvH4_6g43941 FvH4_6g43961
rosa_chinensis RchiOBHm_Chr4g0387951 RchiOBHm_Chr4g0388001 RchiOBHm_Chr4g0388011 RchiOBHm_Chr4g0388051 RchiOBHm_Chr4g0388631 RchiOBHm_Chr4g0388641 RchiOBHm_Chr4g0388661 RchiOBHm_Chr4g0388801
rosa_laevigata RLG00000010028 RLG00000010030 RLG00000010032 RLG00000010082 RLG00000010086 RLG00000010089 RLG00000010091 RLG00000014277
rosa_multiflora Rmu_co8138914.1_g000001 Rmu_sc0000697.1_g000015 Rmu_sc0002180.1_g000030 Rmu_sc0002509.1_g000011 Rmu_sc0002509.1_g000012 Rmu_sc0002509.1_g000020 Rmu_sc0002509.1_g000021 Rmu_sc0004459.1_g000003 Rmu_sc0004459.1_g000005 Rmu_sc0006767.1_g000007 Rmu_sc0014755.1_g000003 Rmu_sc0014755.1_g000004 Rmu_sc0036097.1_g000001
rosa_roxburghii Rroxscaffold_2G00089440 Rroxscaffold_3G00220040 Rroxscaffold_5G00334500 Rroxscaffold_5G00334530 Rroxscaffold_5G00334540 Rroxscaffold_5G00335080
rosa_rugosa Rorug02G0485300 Rorug03G0314100 Rorug03G0314200 Rorug03G0314500 Rorug03G0314500 Rorug03G0314600 Rorug03G0319800 Rorug03G0319900 Rorug03G0320000 Rorug06G0011400
rosa_samantha Rh4AG019700 Rh4AG019900 Rh4AG020200 Rh4AG023900 Rh4AG024000 Rh4AG024700 Rh4BG013900 Rh4BG014100 Rh4BG014200 Rh4BG014300 Rh4BG014400 Rh4BG014500 Rh4BG014600 Rh4BG014700 Rh4BG017100 Rh4BG017400 Rh4BG017500 Rh4BG017600 Rh4BG017700 Rh4BG089200 Rh4DG014500 Rh4DG014800 Rh4DG014900 Rh4DG015400 Rh4DG017900 Rh4DG018000 Rh6BG127900 Rh6CG126300 Rh6DG113400
rosa_wichuraiana Rw4G001270 Rw4G001320 Rw4G001350 Rw4G001700 Rw4G001710 Rw4G001720 Rw4G001760 Rw6G011430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 549
Acc36I ACCTGC 1 cut(s) 48
AccB1I GGYRCC 2 cut(s) 648, 701
AccB7I CCANNNNNTGG 1 cut(s) 108
AccII CGCG 1 cut(s) 26
AciI CCGC 1 cut(s) 145
AclWI GGATC 3 cut(s) 46, 76, 485
AcsI RAATTY 2 cut(s) 416, 785
AcuI CTGAAG 2 cut(s) 305, 837
AcyI GRCGYC 1 cut(s) 702
AfaI GTAC 2 cut(s) 212, 941
AfiI CCNNNNNNNGG 6 cut(s) 61, 108, 312, 362, 597, 1114
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 6 cut(s) 91, 217, 268, 371, 1024, 1164
AjnI CCWGG 2 cut(s) 250, 873
AjuI GAANNNNNNNTTGG 2 cut(s) 825, 857
AleI CACNNNNGTG 2 cut(s) 253, 1038
AluBI AGCT 6 cut(s) 71, 182, 756, 803, 857, 1067
AluI AGCT 6 cut(s) 71, 182, 756, 803, 857, 1067
Alw26I GTCTC 3 cut(s) 103, 732, 992
AlwI GGATC 3 cut(s) 46, 76, 485
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 3 cut(s) 334, 669, 829
ApeKI GCWGC 2 cut(s) 811, 1064
ApoI RAATTY 2 cut(s) 416, 785
Asp700I GAANNNNTTC 2 cut(s) 263, 533
AspLEI GCGC 3 cut(s) 474, 704, 722
AsuC2I CCSGG 2 cut(s) 422, 1074
AsuHPI GGTGA 1 cut(s) 529
AvaI CYCGRG 1 cut(s) 131
BanI GGYRCC 2 cut(s) 648, 701
BanII GRGCYC 1 cut(s) 427
BbsI GAAGAC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 798, 1076
BccI CCATC 2 cut(s) 230, 988
BceAI ACGGC 1 cut(s) 733
BciT130I CCWGG 2 cut(s) 252, 875
BcnI CCSGG 2 cut(s) 422, 1074
BcoDI GTCTC 3 cut(s) 103, 732, 992
BfaI CTAG 2 cut(s) 192, 357
BfmI CTRYAG 1 cut(s) 685
BfoI RGCGCY 1 cut(s) 705
BfrI CTTAAG 1 cut(s) 44
BfuAI ACCTGC 1 cut(s) 48
BglII AGATCT 2 cut(s) 359, 1168
BisI GCNGC 3 cut(s) 145, 812, 1065
BlsI GCNGC 3 cut(s) 146, 813, 1066
BmcAI AGTACT 1 cut(s) 212
Bme1390I CCNGG 4 cut(s) 252, 422, 875, 1074
BmeT110I CYCGRG 1 cut(s) 131
BmiI GGNNCC 3 cut(s) 66, 650, 703
BmrFI CCNGG 4 cut(s) 252, 422, 875, 1074
BmsI GCATC 1 cut(s) 613
BpiI GAAGAC 1 cut(s) 42
BplI GAGNNNNNCTC 2 cut(s) 1112, 1144
BpuMI CCSGG 2 cut(s) 422, 1074
BsaBI GATNNNNATC 1 cut(s) 1050
BsaHI GRCGYC 1 cut(s) 702
BsaJI CCNNGG 2 cut(s) 251, 873
BsaWI WCCGGW 1 cut(s) 590
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 108, 312, 362, 597, 1114
Bse1I ACTGG 1 cut(s) 437
Bse3DI GCAATG 3 cut(s) 113, 337, 961
Bse8I GATNNNNATC 1 cut(s) 1050
BseBI CCWGG 2 cut(s) 252, 875
BseDI CCNNGG 2 cut(s) 251, 873
BseGI GGATG 2 cut(s) 222, 382
BseJI GATNNNNATC 1 cut(s) 1050
BseLI CCNNNNNNNGG 6 cut(s) 61, 108, 312, 362, 597, 1114
BseMI GCAATG 3 cut(s) 113, 337, 961
BseMII CTCAG 2 cut(s) 192, 920
BseNI ACTGG 1 cut(s) 437
BseRI GAGGAG 2 cut(s) 855, 1133
BseXI GCAGC 2 cut(s) 798, 1076
BseYI CCCAGC 1 cut(s) 626
BsgI GTGCAG 1 cut(s) 797
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 3 cut(s) 336, 671, 831
BshNI GGYRCC 2 cut(s) 648, 701
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 3 cut(s) 421, 591, 1074
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 6 cut(s) 61, 108, 312, 362, 597, 1114
BsmAI GTCTC 3 cut(s) 103, 732, 992
BsmBI CGTCTC 2 cut(s) 103, 992
BsmFI GGGAC 1 cut(s) 278
BsmI GAATGC 1 cut(s) 1093
BsnI GGCC 3 cut(s) 336, 671, 831
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 427
Bsp143I GATC 7 cut(s) 51, 81, 359, 490, 1045, 1168, 1189
Bsp68I TCGCGA 1 cut(s) 26
BspACI CCGC 1 cut(s) 145
BspANI GGCC 3 cut(s) 336, 671, 831
BspCNI CTCAG 2 cut(s) 191, 919
BspFNI CGCG 1 cut(s) 26
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 3 cut(s) 66, 650, 703
BspMAI CTGCAG 1 cut(s) 689
BspMI ACCTGC 1 cut(s) 48
BspPI GGATC 3 cut(s) 46, 76, 485
BspQI GCTCTTC 1 cut(s) 1074
BspT107I GGYRCC 2 cut(s) 648, 701
BspTI CTTAAG 1 cut(s) 44
BsrDI GCAATG 3 cut(s) 113, 337, 961
BsrI ACTGG 1 cut(s) 437
BssECI CCNNGG 2 cut(s) 251, 873
BssMI GATC 7 cut(s) 51, 81, 359, 490, 1045, 1168, 1189
BssNI GRCGYC 1 cut(s) 702
Bst2UI CCWGG 2 cut(s) 252, 875
Bst4CI ACNGT 3 cut(s) 22, 782, 897
Bst6I CTCTTC 1 cut(s) 1074
BstACI GRCGYC 1 cut(s) 702
BstAFI CTTAAG 1 cut(s) 44
BstC8I GCNNGC 3 cut(s) 647, 754, 758
BstDEI CTNAG 4 cut(s) 178, 585, 906, 1218
BstEII GGTNACC 1 cut(s) 233
BstF5I GGATG 2 cut(s) 222, 382
BstFNI CGCG 1 cut(s) 26
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 3 cut(s) 474, 704, 722
BstKTI GATC 7 cut(s) 54, 84, 362, 493, 1048, 1171, 1192
BstMAI GTCTC 3 cut(s) 103, 732, 992
BstMBI GATC 7 cut(s) 51, 81, 359, 490, 1045, 1168, 1189
BstMWI GCNNNNNNNGC 1 cut(s) 699
BstNI CCWGG 2 cut(s) 252, 875
BstNSI RCATGY 1 cut(s) 1186
BstPI GGTNACC 1 cut(s) 233
BstSCI CCNGG 4 cut(s) 250, 420, 873, 1072
BstSFI CTRYAG 1 cut(s) 685
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 2 cut(s) 798, 1076
BstV2I GAAGAC 1 cut(s) 42
BstX2I RGATCY 3 cut(s) 51, 359, 1168
BstXI CCANNNNNNTGG 1 cut(s) 255
BstYI RGATCY 3 cut(s) 51, 359, 1168
BsuRI GGCC 3 cut(s) 336, 671, 831
BtsCI GGATG 2 cut(s) 222, 382
BtuMI TCGCGA 1 cut(s) 26
BveI ACCTGC 1 cut(s) 48
Cac8I GCNNGC 3 cut(s) 647, 754, 758
CciI TCATGA 1 cut(s) 660
CfoI GCGC 3 cut(s) 474, 704, 722
CseI GACGC 1 cut(s) 85
Csp6I GTAC 2 cut(s) 211, 940
CspCI CAANNNNNGTGG 2 cut(s) 882, 917
CviAII CATG 6 cut(s) 230, 239, 661, 673, 988, 1183
CviQI GTAC 2 cut(s) 211, 940
DdeI CTNAG 4 cut(s) 178, 585, 906, 1218
DinI GGCGCC 1 cut(s) 703
DpnI GATC 7 cut(s) 53, 83, 361, 492, 1047, 1170, 1191
DpnII GATC 7 cut(s) 51, 81, 359, 490, 1045, 1168, 1189
Eam1104I CTCTTC 1 cut(s) 1074
EarI CTCTTC 1 cut(s) 1074
Eco24I GRGCYC 1 cut(s) 427
Eco57I CTGAAG 2 cut(s) 305, 837
Eco88I CYCGRG 1 cut(s) 131
Eco91I GGTNACC 1 cut(s) 233
EcoO65I GGTNACC 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 416
EcoRII CCWGG 2 cut(s) 250, 873
EcoT38I GRGCYC 1 cut(s) 427
EgeI GGCGCC 1 cut(s) 703
EheI GGCGCC 1 cut(s) 703
Esp3I CGTCTC 2 cut(s) 103, 992
FaeI CATG 6 cut(s) 233, 242, 664, 676, 991, 1186
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 1 cut(s) 278
FatI CATG 6 cut(s) 229, 238, 660, 672, 987, 1182
Fnu4HI GCNGC 3 cut(s) 145, 812, 1065
FokI GGATG 2 cut(s) 209, 369
FriOI GRGCYC 1 cut(s) 427
Fsp4HI GCNGC 3 cut(s) 145, 812, 1065
FspBI CTAG 2 cut(s) 192, 357
GlaI GCGC 3 cut(s) 473, 703, 721
GluI GCNGC 3 cut(s) 145, 812, 1065
GsaI CCCAGC 1 cut(s) 630
HaeII RGCGCY 1 cut(s) 705
HaeIII GGCC 3 cut(s) 336, 671, 831
HapII CCGG 3 cut(s) 421, 591, 1074
HgaI GACGC 1 cut(s) 85
HhaI GCGC 3 cut(s) 474, 704, 722
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 6 cut(s) 233, 242, 664, 676, 991, 1186
Hin6I GCGC 3 cut(s) 472, 702, 720
HinP1I GCGC 3 cut(s) 472, 702, 720
HindIII AAGCTT 1 cut(s) 754
HinfI GANTC 9 cut(s) 259, 287, 302, 316, 458, 904, 1157, 1204, 1210
HpaII CCGG 3 cut(s) 421, 591, 1074
HphI GGTGA 1 cut(s) 529
Hpy188I TCNGA 9 cut(s) 81, 127, 158, 286, 446, 637, 909, 1056, 1209
Hpy188III TCNNGA 4 cut(s) 25, 363, 608, 661
Hpy99I CGWCG 3 cut(s) 26, 144, 641
HpyAV CCTTC 4 cut(s) 236, 274, 504, 692
HpyCH4III ACNGT 3 cut(s) 22, 782, 897
HpyCH4V TGCA 8 cut(s) 330, 676, 687, 714, 814, 966, 1064, 1180
HpyF10VI GCNNNNNNNGC 1 cut(s) 699
HpyF3I CTNAG 4 cut(s) 178, 585, 906, 1218
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 6 cut(s) 233, 242, 664, 676, 991, 1186
HspAI GCGC 3 cut(s) 472, 702, 720
KasI GGCGCC 1 cut(s) 701
Kzo9I GATC 7 cut(s) 51, 81, 359, 490, 1045, 1168, 1189
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 1 cut(s) 690
Lsp1109I GCAGC 2 cut(s) 798, 1076
LweI GCATC 1 cut(s) 613
MaeI CTAG 2 cut(s) 192, 357
MaeIII GTNAC 1 cut(s) 233
MalI GATC 7 cut(s) 53, 83, 361, 492, 1047, 1170, 1191
MboI GATC 7 cut(s) 51, 81, 359, 490, 1045, 1168, 1189
MboII GAAGA 6 cut(s) 47, 178, 311, 613, 1061, 1152
MflI RGATCY 3 cut(s) 51, 359, 1168
MhlI GDGCHC 1 cut(s) 427
MluCI AATT 3 cut(s) 280, 416, 785
Mly113I GGCGCC 1 cut(s) 702
MlyI GAGTC 2 cut(s) 898, 1198
MmeI TCCRAC 2 cut(s) 150, 660
MroXI GAANNNNTTC 2 cut(s) 263, 533
MseI TTAA 3 cut(s) 45, 276, 1050
MslI CAYNNNNRTG 4 cut(s) 253, 453, 992, 1038
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 3 cut(s) 421, 591, 1074
MspR9I CCNGG 4 cut(s) 252, 422, 875, 1074
Mva1269I GAATGC 1 cut(s) 1093
MvaI CCWGG 2 cut(s) 252, 875
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 1 cut(s) 699
NarI GGCGCC 1 cut(s) 702
NciI CCSGG 2 cut(s) 422, 1074
NdeII GATC 7 cut(s) 51, 81, 359, 490, 1045, 1168, 1189
NlaIII CATG 6 cut(s) 233, 242, 664, 676, 991, 1186
NlaIV GGNNCC 3 cut(s) 66, 650, 703
NruI TCGCGA 1 cut(s) 26
NspI RCATGY 1 cut(s) 1186
OliI CACNNNNGTG 2 cut(s) 253, 1038
PagI TCATGA 1 cut(s) 660
PciSI GCTCTTC 1 cut(s) 1074
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 1036
PctI GAATGC 1 cut(s) 1093
PdmI GAANNNNTTC 2 cut(s) 263, 533
PfeI GAWTC 7 cut(s) 259, 287, 302, 316, 458, 1157, 1210
PflMI CCANNNNNTGG 1 cut(s) 108
PfoI TCCNGGA 1 cut(s) 1072
PkrI GCNGC 3 cut(s) 146, 813, 1066
PleI GAGTC 2 cut(s) 898, 1198
PluTI GGCGCC 1 cut(s) 705
PpsI GAGTC 2 cut(s) 898, 1198
PsiI TTATAA 1 cut(s) 549
Psp6I CCWGG 2 cut(s) 250, 873
PspEI GGTNACC 1 cut(s) 233
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 2 cut(s) 250, 873
PspN4I GGNNCC 3 cut(s) 66, 650, 703
PstI CTGCAG 1 cut(s) 689
PsuI RGATCY 3 cut(s) 51, 359, 1168
RruI TCGCGA 1 cut(s) 26
RsaI GTAC 2 cut(s) 212, 941
RsaNI GTAC 2 cut(s) 211, 940
RseI CAYNNNNRTG 4 cut(s) 253, 453, 992, 1038
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 3 cut(s) 45, 276, 1050
SatI GCNGC 3 cut(s) 145, 812, 1065
Sau3AI GATC 7 cut(s) 51, 81, 359, 490, 1045, 1168, 1189
ScaI AGTACT 1 cut(s) 212
SchI GAGTC 2 cut(s) 898, 1198
ScrFI CCNGG 4 cut(s) 252, 422, 875, 1074
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 1 cut(s) 685
SfoI GGCGCC 1 cut(s) 703
SmiMI CAYNNNNRTG 4 cut(s) 253, 453, 992, 1038
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 3 cut(s) 280, 416, 785
SsiI CCGC 1 cut(s) 145
SspDI GGCGCC 1 cut(s) 701
SspI AATATT 1 cut(s) 977
SspMI CTAG 2 cut(s) 192, 357
StyD4I CCNGG 4 cut(s) 250, 420, 873, 1072
TaaI ACNGT 3 cut(s) 22, 782, 897
TaqI TCGA 5 cut(s) 33, 609, 822, 1030, 1192
TasI AATT 3 cut(s) 280, 416, 785
TatI WGTACW 2 cut(s) 210, 939
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 7 cut(s) 259, 287, 302, 316, 458, 1157, 1210
Tru1I TTAA 3 cut(s) 45, 276, 1050
Tru9I TTAA 3 cut(s) 45, 276, 1050
TseI GCWGC 2 cut(s) 811, 1064
TspDTI ATGAA 8 cut(s) 218, 255, 315, 570, 649, 677, 716, 778
TspGWI ACGGA 4 cut(s) 74, 128, 280, 1016
Van91I CCANNNNNTGG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 44
XapI RAATTY 2 cut(s) 416, 785
XceI RCATGY 1 cut(s) 1186
XmnI GAANNNNTTC 2 cut(s) 263, 533
XspI CTAG 2 cut(s) 192, 357
ZrmI AGTACT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.