Rh4AG019700

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000449
Physical Location & Seq
Reverse (-)
3769511 .. 3770842
1332 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG019700.1

Sequence Viewer

Length: 1332 bp
ATGTCATCTTCAGCAGAAACGGTAGCGAACATCGAAGGGCTCCTTAAGCAGATCCTTATATCGGTGCCGGCTCTATCTCTGATCCGCTTCAAGTGCGTCTCCAAGCACTGGCTCTCTCTCATTTCCGACCCCGACTCCTGTCGCCGCCACACTCTCCGAAACCCTAACCCCATAATCTCAGCTTTCTTCTCTTCCCAAACCAATGAAGACACCACCATCAACTCCATCACTCTCGGCAGCCCATCCGGTAATCCCTTCAAAACCCTCGCCAATTCCGCCTCCGCGTTGAGGATTCTCCAGTCCTGCAATGGCCTCTTCCTCTGCTGTGTCGAATTCCCCGGAGAAGAAGAAGAAAGAAACTATCCTCCTGTATATGTCGTCAATCCCACCACCAACAAATTCCGCGCTCTTTCTGCTCCAAGCCTTGCGAAGGAGGAAATGTATATGTTTGTGAGGTATGGTTTGGCTTTCGACCCTTCGAAATCGCCTCATTACACTGTGGTGTGTGTCAGTCACGACATTCTCCGTGGCAAGCGCCACAGCATAGAGATATATTCGTCGGAGACCGGAACTTGGGTGAAGCGTTTCAAGGCTCTTTTCTTCCCTAGCCCTTCCGACGAGGGCAGGCACATTGATACTCGCAAAATGCCTATGCATTTTGATGAGAGGAGCAGGGAAGGGGCAGTGTATTGCAACGGCGCAATTCATTGGATAAGAAACAGATGGGAAGCGAGCCTGCCATTCTGCTCGGAGACAGATGGTGTGGTCGAAACGGTTGGATTCGAAAGAAGGAAATTGGATGTGTTGCACTACTATGACATCGGTGAAGAAAGTCTGCGGCTTGCTGCTGCTACTCCTCCAGTTCCTTTGGTTGTGAAAAACATTCCGTTGGAGAAAGAACAAAACTTTCCTACTGAGTTTCCCCATTTGATTGATAGATACATTGGCGAGTCAGGTGGTGGCTGTTTGTATTTGATTGAGGTTTTTGAGCATTGTAAGACTCAATTTGAGGTGATGGAGATGGAGAGGGACTACTCTCGATGGTTTGTCAAGTACCATGTTGATCTTAGTCAGGTAGTCGCACCTCTCCCTGGACAGGATCCAAATGCTTTTGTTGTCTTGTTCCTTTCAGGAGACGTGGAAACAGATGGTGAGGATTCTTCGACTGATCTTTGGTTGCATATGCCGGGTAAGGTCGTATCTTATAATCTCAGGAATAAGAGCTTCAAGACATCTGTTGAGTTAGCCAATAGGGAACTTTTTCTAGCTTTAGATTATGATGAATATGAGGATGACAACTATCCTTACATGGAGACTTTAGCTTGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

443

Amino Acids

50.34

Weight (kDa)

5.33

Isoelectric Point (pI)

63.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_1 PF07734 97 - 194 7.9e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12890 FvH4_4g01370 FvH4_4g01420 FvH4_4g01430 FvH4_4g01472 FvH4_4g01490 FvH4_6g31140 FvH4_6g31150 FvH4_6g31161 FvH4_6g43941 FvH4_6g43961
rosa_chinensis RchiOBHm_Chr4g0387951 RchiOBHm_Chr4g0388001 RchiOBHm_Chr4g0388011 RchiOBHm_Chr4g0388051 RchiOBHm_Chr4g0388631 RchiOBHm_Chr4g0388641 RchiOBHm_Chr4g0388661 RchiOBHm_Chr4g0388801
rosa_laevigata RLG00000010028 RLG00000010030 RLG00000010032 RLG00000010082 RLG00000010086 RLG00000010089 RLG00000010091 RLG00000014277
rosa_multiflora Rmu_co8138914.1_g000001 Rmu_sc0000697.1_g000015 Rmu_sc0002180.1_g000030 Rmu_sc0002509.1_g000011 Rmu_sc0002509.1_g000012 Rmu_sc0002509.1_g000020 Rmu_sc0002509.1_g000021 Rmu_sc0004459.1_g000003 Rmu_sc0004459.1_g000005 Rmu_sc0006767.1_g000007 Rmu_sc0014755.1_g000003 Rmu_sc0014755.1_g000004 Rmu_sc0036097.1_g000001
rosa_roxburghii Rroxscaffold_2G00089440 Rroxscaffold_3G00220040 Rroxscaffold_5G00334500 Rroxscaffold_5G00334530 Rroxscaffold_5G00334540 Rroxscaffold_5G00335080
rosa_rugosa Rorug02G0485300 Rorug03G0314100 Rorug03G0314200 Rorug03G0314500 Rorug03G0314500 Rorug03G0314600 Rorug03G0319800 Rorug03G0319900 Rorug03G0320000 Rorug06G0011400
rosa_samantha Rh4AG019700 Rh4AG019900 Rh4AG020200 Rh4AG023900 Rh4AG024000 Rh4AG024700 Rh4BG013900 Rh4BG014100 Rh4BG014200 Rh4BG014300 Rh4BG014400 Rh4BG014500 Rh4BG014600 Rh4BG014700 Rh4BG017100 Rh4BG017400 Rh4BG017500 Rh4BG017600 Rh4BG017700 Rh4BG089200 Rh4DG014500 Rh4DG014800 Rh4DG014900 Rh4DG015400 Rh4DG017900 Rh4DG018000 Rh6BG127900 Rh6CG126300 Rh6DG113400
rosa_wichuraiana Rw4G001270 Rw4G001320 Rw4G001350 Rw4G001700 Rw4G001710 Rw4G001720 Rw4G001760 Rw6G011430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1206
AccB1I GGYRCC 1 cut(s) 64
AccB7I CCANNNNNTGG 1 cut(s) 108
AccII CGCG 2 cut(s) 284, 405
AciI CCGC 6 cut(s) 85, 145, 276, 282, 403, 838
AclWI GGATC 4 cut(s) 46, 76, 1094, 1107
AcsI RAATTY 2 cut(s) 332, 398
AfaI GTAC 1 cut(s) 1055
AfiI CCNNNNNNNGG 7 cut(s) 61, 108, 288, 430, 573, 1091, 1096
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 4 cut(s) 91, 259, 589, 1228
AjiI CACGTC 1 cut(s) 1138
AjnI CCWGG 1 cut(s) 1090
AleI CACNNNNGTG 1 cut(s) 500
AluBI AGCT 4 cut(s) 182, 1224, 1268, 1322
AluI AGCT 4 cut(s) 182, 1224, 1268, 1322
Alw26I GTCTC 5 cut(s) 103, 557, 746, 1128, 1307
AlwI GGATC 4 cut(s) 46, 76, 1094, 1107
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 3 cut(s) 237, 845, 848
ApoI RAATTY 2 cut(s) 332, 398
Asp700I GAANNNNTTC 2 cut(s) 584, 1260
AspLEI GCGC 3 cut(s) 407, 537, 701
AsuC2I CCSGG 2 cut(s) 339, 1188
AsuHPI GGTGA 4 cut(s) 589, 836, 1024, 1163
AsuII TTCGAA 2 cut(s) 479, 783
BamHI GGATCC 1 cut(s) 1099
BanI GGYRCC 1 cut(s) 64
BanII GRGCYC 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 213
BbvI GCAGC 3 cut(s) 249, 832, 835
BccI CCATC 9 cut(s) 224, 233, 250, 717, 752, 1009, 1015, 1035, 1142
BceAI ACGGC 1 cut(s) 712
BciT130I CCWGG 1 cut(s) 1092
BcnI CCSGG 2 cut(s) 339, 1188
BcoDI GTCTC 5 cut(s) 103, 557, 746, 1128, 1307
BfaI CTAG 2 cut(s) 606, 1265
BfoI RGCGCY 1 cut(s) 538
BfrI CTTAAG 1 cut(s) 44
BisI GCNGC 5 cut(s) 145, 238, 839, 846, 849
BlsI GCNGC 5 cut(s) 146, 239, 840, 847, 850
Bme1390I CCNGG 3 cut(s) 339, 1092, 1188
BmgBI CACGTC 1 cut(s) 1138
BmiI GGNNCC 3 cut(s) 41, 66, 1101
BmrFI CCNGG 3 cut(s) 339, 1092, 1188
BoxI GACNNNNGTC 1 cut(s) 138
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 2 cut(s) 281, 843
Bpu14I TTCGAA 2 cut(s) 479, 783
BpuMI CCSGG 2 cut(s) 339, 1188
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 3 cut(s) 337, 526, 1090
BsaWI WCCGGW 2 cut(s) 245, 566
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 425, 455
Bsc4I CCNNNNNNNGG 7 cut(s) 61, 108, 288, 430, 573, 1091, 1096
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 3 cut(s) 113, 298, 860
Bse3DI GCAATG 1 cut(s) 313
BseBI CCWGG 1 cut(s) 1092
BseDI CCNNGG 3 cut(s) 337, 526, 1090
BseGI GGATG 3 cut(s) 242, 805, 1297
BseLI CCNNNNNNNGG 7 cut(s) 61, 108, 288, 430, 573, 1091, 1096
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 3 cut(s) 192, 906, 1225
BseNI ACTGG 3 cut(s) 113, 298, 860
BseRI GAGGAG 2 cut(s) 682, 846
BseXI GCAGC 3 cut(s) 249, 832, 835
Bsh1236I CGCG 2 cut(s) 284, 405
BshFI GGCC 1 cut(s) 312
BshNI GGYRCC 1 cut(s) 64
BsiSI CCGG 5 cut(s) 68, 246, 339, 567, 1187
BslFI GGGAC 1 cut(s) 1043
BslI CCNNNNNNNGG 7 cut(s) 61, 108, 288, 430, 573, 1091, 1096
BsmAI GTCTC 5 cut(s) 103, 557, 746, 1128, 1307
BsmBI CGTCTC 2 cut(s) 103, 1128
BsmFI GGGAC 1 cut(s) 1043
BsnI GGCC 1 cut(s) 312
Bso31I GGTCTC 1 cut(s) 557
Bsp119I TTCGAA 2 cut(s) 479, 783
Bsp1286I GDGCHC 1 cut(s) 42
Bsp143I GATC 5 cut(s) 51, 81, 1063, 1099, 1168
BspACI CCGC 6 cut(s) 85, 145, 276, 282, 403, 838
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 3 cut(s) 191, 907, 1224
BspFNI CGCG 2 cut(s) 284, 405
BspLI GGNNCC 3 cut(s) 41, 66, 1101
BspPI GGATC 4 cut(s) 46, 76, 1094, 1107
BspT104I TTCGAA 2 cut(s) 479, 783
BspT107I GGYRCC 1 cut(s) 64
BspTI CTTAAG 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 313
BsrFI RCCGGY 1 cut(s) 67
BsrI ACTGG 3 cut(s) 113, 298, 860
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 3 cut(s) 337, 526, 1090
BssMI GATC 5 cut(s) 51, 81, 1063, 1099, 1168
Bst2UI CCWGG 1 cut(s) 1092
Bst4CI ACNGT 3 cut(s) 22, 499, 775
Bst6I CTCTTC 2 cut(s) 196, 320
BstAFI CTTAAG 1 cut(s) 44
BstBI TTCGAA 2 cut(s) 479, 783
BstC8I GCNNGC 6 cut(s) 69, 533, 626, 733, 737, 843
BstDEI CTNAG 4 cut(s) 178, 915, 1067, 1211
BstDSI CCRYGG 1 cut(s) 526
BstENI CCTNNNNNAGG 1 cut(s) 428
BstF5I GGATG 3 cut(s) 242, 805, 1297
BstFNI CGCG 2 cut(s) 284, 405
BstH2I RGCGCY 1 cut(s) 538
BstHHI GCGC 3 cut(s) 407, 537, 701
BstKTI GATC 5 cut(s) 54, 84, 1066, 1102, 1171
BstMAI GTCTC 5 cut(s) 103, 557, 746, 1128, 1307
BstMBI GATC 5 cut(s) 51, 81, 1063, 1099, 1168
BstMWI GCNNNNNNNGC 4 cut(s) 46, 93, 275, 413
BstNI CCWGG 1 cut(s) 1092
BstPAI GACNNNNGTC 1 cut(s) 138
BstSCI CCNGG 3 cut(s) 337, 1090, 1186
BstUI CGCG 2 cut(s) 284, 405
BstV1I GCAGC 3 cut(s) 249, 832, 835
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 2 cut(s) 51, 1099
BstYI RGATCY 2 cut(s) 51, 1099
BsuRI GGCC 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 526
BtrI CACGTC 1 cut(s) 1138
BtsCI GGATG 3 cut(s) 242, 805, 1297
BtsI GCAGTG 1 cut(s) 690
BtsIMutI CAGTG 3 cut(s) 106, 495, 690
Cac8I GCNNGC 6 cut(s) 69, 533, 626, 733, 737, 843
CfoI GCGC 3 cut(s) 407, 537, 701
Cfr10I RCCGGY 1 cut(s) 67
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 1054
CviAII CATG 2 cut(s) 1058, 1309
CviQI GTAC 1 cut(s) 1054
DdeI CTNAG 4 cut(s) 178, 915, 1067, 1211
DpnI GATC 5 cut(s) 53, 83, 1065, 1101, 1170
DpnII GATC 5 cut(s) 51, 81, 1063, 1099, 1168
Eam1104I CTCTTC 2 cut(s) 196, 320
EarI CTCTTC 2 cut(s) 196, 320
EciI GGCGGA 1 cut(s) 265
Eco24I GRGCYC 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 557
EcoNI CCTNNNNNAGG 1 cut(s) 428
EcoRI GAATTC 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 1090
EcoT22I ATGCAT 1 cut(s) 657
EcoT38I GRGCYC 1 cut(s) 42
Esp3I CGTCTC 2 cut(s) 103, 1128
FaeI CATG 2 cut(s) 1061, 1312
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 1 cut(s) 1043
FatI CATG 2 cut(s) 1057, 1308
FauNDI CATATG 1 cut(s) 1182
Fnu4HI GCNGC 5 cut(s) 145, 238, 839, 846, 849
FokI GGATG 3 cut(s) 229, 812, 1304
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 5 cut(s) 145, 238, 839, 846, 849
FspBI CTAG 2 cut(s) 606, 1265
GlaI GCGC 3 cut(s) 406, 536, 700
GluI GCNGC 5 cut(s) 145, 238, 839, 846, 849
GsuI CTGGAG 2 cut(s) 281, 843
HaeII RGCGCY 1 cut(s) 538
HaeIII GGCC 1 cut(s) 312
HapII CCGG 5 cut(s) 68, 246, 339, 567, 1187
HgaI GACGC 1 cut(s) 85
HhaI GCGC 3 cut(s) 407, 537, 701
Hin1II CATG 2 cut(s) 1061, 1312
Hin6I GCGC 3 cut(s) 405, 535, 699
HinP1I GCGC 3 cut(s) 405, 535, 699
HinfI GANTC 6 cut(s) 134, 292, 780, 950, 1000, 1157
HpaII CCGG 5 cut(s) 68, 246, 339, 567, 1187
HphI GGTGA 4 cut(s) 589, 836, 1024, 1163
Hpy188I TCNGA 6 cut(s) 81, 127, 158, 562, 616, 751
Hpy188III TCNNGA 5 cut(s) 515, 1038, 1131, 1213, 1228
Hpy99I CGWCG 2 cut(s) 562, 620
HpyAV CCTTC 7 cut(s) 29, 265, 424, 486, 621, 671, 783
HpyCH4III ACNGT 3 cut(s) 22, 499, 775
HpyCH4IV ACGT 1 cut(s) 1137
HpyCH4V TGCA 5 cut(s) 306, 655, 693, 808, 1180
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 93, 275, 413
HpyF3I CTNAG 4 cut(s) 178, 915, 1067, 1211
HpySE526I ACGT 1 cut(s) 1137
Hsp92II CATG 2 cut(s) 1061, 1312
HspAI GCGC 3 cut(s) 405, 535, 699
KroI GCCGGC 1 cut(s) 67
KroNI GCCGGC 1 cut(s) 69
Kzo9I GATC 5 cut(s) 51, 81, 1063, 1099, 1168
LmnI GCTCC 3 cut(s) 45, 421, 669
Lsp1109I GCAGC 3 cut(s) 249, 832, 835
MaeI CTAG 2 cut(s) 606, 1265
MaeII ACGT 1 cut(s) 1137
MaeIII GTNAC 1 cut(s) 512
MalI GATC 5 cut(s) 53, 83, 1065, 1101, 1170
MboI GATC 5 cut(s) 51, 81, 1063, 1099, 1168
MflI RGATCY 2 cut(s) 51, 1099
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 6 cut(s) 271, 332, 398, 702, 794, 1004
MlyI GAGTC 3 cut(s) 128, 959, 994
MmeI TCCRAC 5 cut(s) 150, 540, 639, 757, 870
Mph1103I ATGCAT 1 cut(s) 657
MroNI GCCGGC 1 cut(s) 67
MroXI GAANNNNTTC 2 cut(s) 584, 1260
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 3 cut(s) 500, 660, 813
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 5 cut(s) 68, 246, 339, 567, 1187
MspR9I CCNGG 3 cut(s) 339, 1092, 1188
MvaI CCWGG 1 cut(s) 1092
MvnI CGCG 2 cut(s) 284, 405
MwoI GCNNNNNNNGC 4 cut(s) 46, 93, 275, 413
NaeI GCCGGC 1 cut(s) 69
NciI CCSGG 2 cut(s) 339, 1188
NdeI CATATG 1 cut(s) 1182
NdeII GATC 5 cut(s) 51, 81, 1063, 1099, 1168
NgoMIV GCCGGC 1 cut(s) 67
NlaIII CATG 2 cut(s) 1061, 1312
NlaIV GGNNCC 3 cut(s) 41, 66, 1101
NmeAIII GCCGAG 1 cut(s) 213
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 657
NspV TTCGAA 2 cut(s) 479, 783
OliI CACNNNNGTG 1 cut(s) 500
PdiI GCCGGC 1 cut(s) 69
PdmI GAANNNNTTC 2 cut(s) 584, 1260
PfeI GAWTC 3 cut(s) 292, 780, 1157
PflMI CCANNNNNTGG 1 cut(s) 108
PkrI GCNGC 5 cut(s) 146, 239, 840, 847, 850
PleI GAGTC 3 cut(s) 128, 958, 994
PpsI GAGTC 3 cut(s) 128, 958, 994
PshAI GACNNNNGTC 1 cut(s) 138
PsiI TTATAA 1 cut(s) 1206
Psp6I CCWGG 1 cut(s) 1090
PspGI CCWGG 1 cut(s) 1090
PspN4I GGNNCC 3 cut(s) 41, 66, 1101
PsuI RGATCY 2 cut(s) 51, 1099
RsaI GTAC 1 cut(s) 1055
RsaNI GTAC 1 cut(s) 1054
RseI CAYNNNNRTG 3 cut(s) 500, 660, 813
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 5 cut(s) 145, 238, 839, 846, 849
Sau3AI GATC 5 cut(s) 51, 81, 1063, 1099, 1168
SchI GAGTC 3 cut(s) 128, 959, 994
ScrFI CCNGG 3 cut(s) 339, 1092, 1188
SduI GDGCHC 1 cut(s) 42
SfuI TTCGAA 2 cut(s) 479, 783
SmiMI CAYNNNNRTG 3 cut(s) 500, 660, 813
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 6 cut(s) 271, 332, 398, 702, 794, 1004
SsiI CCGC 6 cut(s) 85, 145, 276, 282, 403, 838
SspMI CTAG 2 cut(s) 606, 1265
StyD4I CCNGG 3 cut(s) 337, 1090, 1186
TaaI ACNGT 3 cut(s) 22, 499, 775
TaiI ACGT 1 cut(s) 1140
TaqI TCGA 8 cut(s) 33, 330, 471, 479, 768, 783, 1039, 1163
TasI AATT 6 cut(s) 271, 332, 398, 702, 794, 1004
TauI GCSGC 2 cut(s) 147, 841
TfiI GAWTC 3 cut(s) 292, 780, 1157
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 3 cut(s) 113, 502, 690
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 3 cut(s) 237, 845, 848
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 3 cut(s) 219, 695, 1296
TspGWI ACGGA 2 cut(s) 515, 876
TspRI CASTG 3 cut(s) 113, 502, 690
Van91I CCANNNNNTGG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 44
XagI CCTNNNNNAGG 1 cut(s) 428
XapI RAATTY 2 cut(s) 332, 398
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmnI GAANNNNTTC 2 cut(s) 584, 1260
XspI CTAG 2 cut(s) 606, 1265
Zsp2I ATGCAT 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.