Rw4G001320

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000449
Physical Location & Seq
Reverse (-)
2399413 .. 2400762
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001320.1

Sequence Viewer

Length: 1200 bp
ATGTCATCTTCAGCAGAAACCGTCGCGAATATCGAAGAGCTGCTTAAGCAGATCCTTATATGGGTTCCAGCTCTATCTCTGATCCGTTTCAAGTGCGTCTCCAAGCAATGGCTCTCTCTCATCTCCGACCCCGAGTTCCGTCGCCGCCACACCCTCCGAAACCCTAACTCCAAAATCTCAGCTTTCTTCTCTAGCAAAATCCAAGACGAATCTGGACTTGAAACAAAAAATCATCCCGTATATGTTGTCAATCCCACAACCAACGAATTCCGGGCTCTTTCTTTTCCAGTTGTCAGACATAGTAGTGATTCTTTATTTGTGCGCTATGCTTTGGCTTTTGATCCTTCCATATCGCCTCATTACAAGGTGGTCTGCGTGAATAACTTCACCTTTTATTATAATAGGGGGAAGCACAAAATAGACATATATTCATCTAAGACCGGAGAGTGGAAGCATCTCGATACTCCTTTCTTCCCCAGACCCTCCGACGAGGGCAGGCACCATCATTTCATGAACAAGGCCATGCACTTTGACTGCAGGAGCAGAGAAGGCGCCATATACTGCAACGGCGCAGTTCATTGGATAAGAGACATAGGGGAAGCAAGCTTGCCATTCTACTCTTTCCCAGACGGTAAATTCATAAGAAACGAAGCTGATGTGCTGCACTACTTCGACATAGGCCAAGAGCGTTTTCTGGTTGCTTCAGCTACCCCTCCTGTCCCCCTGGTTGCCAAGAACTTTCTACCGTTATTGACTCAGAGATATTTTGGGGAGTGTGGTGGCTGTTTGTACTTGATTGAGATTTATCAGCGTTGCAACACTCAATATTTTGAGGTCATGGAGATGGAGACGGACTACTCTGGCTGGTTTGTCAAGTATCGTCACTATCGTGTTATTAGTGTTGATCTTAATCGGATAGTTGCAGCTCTTCCCGGACAGGATTGGAATGCGTTTGTTGTCTTGTGCCTTTCTGGGGAGGAGGAAACCTCCCAAGAAATAGATGACAAGGTTGAGGATTCTTCAACAGATCTTTTGTTGCACATGCCTGGTAAGATCATATCTTATAATCTTAGGAATAAGACCTTCAAGACATCTATTGAGTTAGCCAATAAGGAGCTTTATCTAACTTTAGGTGATCATAATCATTGTTTGCCAGAGGATATTATTTTTCCTTACATGGAGACTTTAGCTTGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

399

Amino Acids

46.16

Weight (kDa)

6.25

Isoelectric Point (pI)

55.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 12 - 48 4.3e-07 F-box domain
FBA_1 PF07734 76 - 232 4.3e-07 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12890 FvH4_4g01370 FvH4_4g01420 FvH4_4g01430 FvH4_4g01472 FvH4_4g01490 FvH4_6g31140 FvH4_6g31150 FvH4_6g31161 FvH4_6g43941 FvH4_6g43961
rosa_chinensis RchiOBHm_Chr4g0387951 RchiOBHm_Chr4g0388001 RchiOBHm_Chr4g0388011 RchiOBHm_Chr4g0388051 RchiOBHm_Chr4g0388631 RchiOBHm_Chr4g0388641 RchiOBHm_Chr4g0388661 RchiOBHm_Chr4g0388801
rosa_laevigata RLG00000010028 RLG00000010030 RLG00000010032 RLG00000010082 RLG00000010086 RLG00000010089 RLG00000010091 RLG00000014277
rosa_multiflora Rmu_co8138914.1_g000001 Rmu_sc0000697.1_g000015 Rmu_sc0002180.1_g000030 Rmu_sc0002509.1_g000011 Rmu_sc0002509.1_g000012 Rmu_sc0002509.1_g000020 Rmu_sc0002509.1_g000021 Rmu_sc0004459.1_g000003 Rmu_sc0004459.1_g000005 Rmu_sc0006767.1_g000007 Rmu_sc0014755.1_g000003 Rmu_sc0014755.1_g000004 Rmu_sc0036097.1_g000001
rosa_roxburghii Rroxscaffold_2G00089440 Rroxscaffold_3G00220040 Rroxscaffold_5G00334500 Rroxscaffold_5G00334530 Rroxscaffold_5G00334540 Rroxscaffold_5G00335080
rosa_rugosa Rorug02G0485300 Rorug03G0314100 Rorug03G0314200 Rorug03G0314500 Rorug03G0314500 Rorug03G0314600 Rorug03G0319800 Rorug03G0319900 Rorug03G0320000 Rorug06G0011400
rosa_samantha Rh4AG019700 Rh4AG019900 Rh4AG020200 Rh4AG023900 Rh4AG024000 Rh4AG024700 Rh4BG013900 Rh4BG014100 Rh4BG014200 Rh4BG014300 Rh4BG014400 Rh4BG014500 Rh4BG014600 Rh4BG014700 Rh4BG017100 Rh4BG017400 Rh4BG017500 Rh4BG017600 Rh4BG017700 Rh4BG089200 Rh4DG014500 Rh4DG014800 Rh4DG014900 Rh4DG015400 Rh4DG017900 Rh4DG018000 Rh6BG127900 Rh6CG126300 Rh6DG113400
rosa_wichuraiana Rw4G001270 Rw4G001320 Rw4G001350 Rw4G001700 Rw4G001710 Rw4G001720 Rw4G001760 Rw6G011430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 399, 1065
AccB1I GGYRCC 2 cut(s) 498, 551
AccB7I CCANNNNNTGG 1 cut(s) 108
AccII CGCG 1 cut(s) 26
AciI CCGC 1 cut(s) 145
AclWI GGATC 3 cut(s) 46, 76, 335
AcsI RAATTY 2 cut(s) 266, 635
AcuI CTGAAG 1 cut(s) 687
AcyI GRCGYC 1 cut(s) 552
AfaI GTAC 1 cut(s) 791
AfiI CCNNNNNNNGG 4 cut(s) 61, 108, 447, 973
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 4 cut(s) 91, 221, 1023, 1087
AjnI CCWGG 2 cut(s) 723, 1045
AjuI GAANNNNNNNTTGG 2 cut(s) 675, 707
AleI CACNNNNGTG 1 cut(s) 888
AluBI AGCT 9 cut(s) 40, 71, 182, 606, 653, 707, 926, 1117, 1190
AluI AGCT 9 cut(s) 40, 71, 182, 606, 653, 707, 926, 1117, 1190
Alw26I GTCTC 4 cut(s) 103, 582, 842, 1175
AlwI GGATC 3 cut(s) 46, 76, 335
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 2 cut(s) 519, 679
ApeKI GCWGC 3 cut(s) 40, 661, 923
ApoI RAATTY 2 cut(s) 266, 635
Asp700I GAANNNNTTC 1 cut(s) 383
AspLEI GCGC 3 cut(s) 324, 554, 572
AsuC2I CCSGG 2 cut(s) 272, 933
AsuHPI GGTGA 2 cut(s) 379, 1145
AvaI CYCGRG 1 cut(s) 131
BanI GGYRCC 2 cut(s) 498, 551
BanII GRGCYC 1 cut(s) 277
BbvI GCAGC 3 cut(s) 27, 648, 935
BccI CCATC 2 cut(s) 510, 838
BceAI ACGGC 1 cut(s) 583
BciT130I CCWGG 2 cut(s) 725, 1047
BclI TGATCA 1 cut(s) 1135
BcnI CCSGG 2 cut(s) 272, 933
BcoDI GTCTC 4 cut(s) 103, 582, 842, 1175
BfaI CTAG 1 cut(s) 192
BfmI CTRYAG 1 cut(s) 535
BfoI RGCGCY 1 cut(s) 555
BfrI CTTAAG 1 cut(s) 44
BglII AGATCT 1 cut(s) 1027
BisI GCNGC 4 cut(s) 41, 145, 662, 924
BlsI GCNGC 4 cut(s) 42, 146, 663, 925
Bme1390I CCNGG 4 cut(s) 272, 725, 933, 1047
BmeT110I CYCGRG 1 cut(s) 131
BmiI GGNNCC 3 cut(s) 66, 500, 553
BmrFI CCNGG 4 cut(s) 272, 725, 933, 1047
BmsI GCATC 1 cut(s) 463
BplI GAGNNNNNCTC 2 cut(s) 971, 1003
BpuMI CCSGG 2 cut(s) 272, 933
BsaBI GATNNNNATC 2 cut(s) 909, 1140
BsaHI GRCGYC 1 cut(s) 552
BsaJI CCNNGG 1 cut(s) 723
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 108, 447, 973
Bse1I ACTGG 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 113
Bse8I GATNNNNATC 2 cut(s) 909, 1140
BseBI CCWGG 2 cut(s) 725, 1047
BseDI CCNNGG 1 cut(s) 723
BseGI GGATG 1 cut(s) 232
BseJI GATNNNNATC 2 cut(s) 909, 1140
BseLI CCNNNNNNNGG 4 cut(s) 61, 108, 447, 973
BseMI GCAATG 1 cut(s) 113
BseMII CTCAG 2 cut(s) 192, 770
BseNI ACTGG 1 cut(s) 287
BseRI GAGGAG 1 cut(s) 992
BseXI GCAGC 3 cut(s) 27, 648, 935
BsgI GTGCAG 1 cut(s) 647
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 2 cut(s) 521, 681
BshNI GGYRCC 2 cut(s) 498, 551
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 3 cut(s) 271, 441, 933
BslFI GGGAC 1 cut(s) 704
BslI CCNNNNNNNGG 4 cut(s) 61, 108, 447, 973
BsmAI GTCTC 4 cut(s) 103, 582, 842, 1175
BsmBI CGTCTC 2 cut(s) 103, 842
BsmFI GGGAC 1 cut(s) 704
BsmI GAATGC 1 cut(s) 952
BsnI GGCC 2 cut(s) 521, 681
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 277
Bsp143I GATC 7 cut(s) 51, 81, 340, 904, 1027, 1053, 1135
Bsp68I TCGCGA 1 cut(s) 26
BspACI CCGC 1 cut(s) 145
BspANI GGCC 2 cut(s) 521, 681
BspCNI CTCAG 2 cut(s) 191, 769
BspFNI CGCG 1 cut(s) 26
BspHI TCATGA 1 cut(s) 510
BspLI GGNNCC 3 cut(s) 66, 500, 553
BspMAI CTGCAG 1 cut(s) 539
BspPI GGATC 3 cut(s) 46, 76, 335
BspQI GCTCTTC 2 cut(s) 30, 933
BspT107I GGYRCC 2 cut(s) 498, 551
BspTI CTTAAG 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 113
BsrI ACTGG 1 cut(s) 287
BssECI CCNNGG 1 cut(s) 723
BssMI GATC 7 cut(s) 51, 81, 340, 904, 1027, 1053, 1135
BssNI GRCGYC 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 725, 1047
Bst4CI ACNGT 3 cut(s) 22, 632, 747
Bst6I CTCTTC 2 cut(s) 30, 933
BstACI GRCGYC 1 cut(s) 552
BstAFI CTTAAG 1 cut(s) 44
BstC8I GCNNGC 3 cut(s) 497, 604, 608
BstDEI CTNAG 4 cut(s) 178, 435, 756, 1070
BstF5I GGATG 1 cut(s) 232
BstFNI CGCG 1 cut(s) 26
BstH2I RGCGCY 1 cut(s) 555
BstHHI GCGC 3 cut(s) 324, 554, 572
BstKTI GATC 7 cut(s) 54, 84, 343, 907, 1030, 1056, 1138
BstMAI GTCTC 4 cut(s) 103, 582, 842, 1175
BstMBI GATC 7 cut(s) 51, 81, 340, 904, 1027, 1053, 1135
BstMWI GCNNNNNNNGC 2 cut(s) 46, 549
BstNI CCWGG 2 cut(s) 725, 1047
BstNSI RCATGY 1 cut(s) 1045
BstSCI CCNGG 4 cut(s) 270, 723, 931, 1045
BstSFI CTRYAG 1 cut(s) 535
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 3 cut(s) 27, 648, 935
BstX2I RGATCY 2 cut(s) 51, 1027
BstYI RGATCY 2 cut(s) 51, 1027
BsuRI GGCC 2 cut(s) 521, 681
BtsCI GGATG 1 cut(s) 232
BtuMI TCGCGA 1 cut(s) 26
Cac8I GCNNGC 3 cut(s) 497, 604, 608
CciI TCATGA 1 cut(s) 510
CfoI GCGC 3 cut(s) 324, 554, 572
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 790
CviAII CATG 5 cut(s) 511, 523, 838, 1042, 1177
CviQI GTAC 1 cut(s) 790
DdeI CTNAG 4 cut(s) 178, 435, 756, 1070
DinI GGCGCC 1 cut(s) 553
DpnI GATC 7 cut(s) 53, 83, 342, 906, 1029, 1055, 1137
DpnII GATC 7 cut(s) 51, 81, 340, 904, 1027, 1053, 1135
Eam1104I CTCTTC 2 cut(s) 30, 933
EarI CTCTTC 2 cut(s) 30, 933
Eco24I GRGCYC 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 687
Eco88I CYCGRG 1 cut(s) 131
EcoRI GAATTC 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 723, 1045
EcoT38I GRGCYC 1 cut(s) 277
EgeI GGCGCC 1 cut(s) 553
EheI GGCGCC 1 cut(s) 553
Esp3I CGTCTC 2 cut(s) 103, 842
FaeI CATG 5 cut(s) 514, 526, 841, 1045, 1180
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 1 cut(s) 704
FatI CATG 5 cut(s) 510, 522, 837, 1041, 1176
FbaI TGATCA 1 cut(s) 1135
Fnu4HI GCNGC 4 cut(s) 41, 145, 662, 924
FokI GGATG 1 cut(s) 219
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 4 cut(s) 41, 145, 662, 924
FspBI CTAG 1 cut(s) 192
GlaI GCGC 3 cut(s) 323, 553, 571
GluI GCNGC 4 cut(s) 41, 145, 662, 924
HaeII RGCGCY 1 cut(s) 555
HaeIII GGCC 2 cut(s) 521, 681
HapII CCGG 3 cut(s) 271, 441, 933
HgaI GACGC 1 cut(s) 85
HhaI GCGC 3 cut(s) 324, 554, 572
Hin1I GRCGYC 1 cut(s) 552
Hin1II CATG 5 cut(s) 514, 526, 841, 1045, 1180
Hin6I GCGC 3 cut(s) 322, 552, 570
HinP1I GCGC 3 cut(s) 322, 552, 570
HindIII AAGCTT 1 cut(s) 604
HinfI GANTC 4 cut(s) 209, 308, 754, 1016
HpaII CCGG 3 cut(s) 271, 441, 933
HphI GGTGA 2 cut(s) 379, 1145
Hpy188I TCNGA 7 cut(s) 81, 127, 158, 296, 487, 759, 915
Hpy188III TCNNGA 5 cut(s) 25, 213, 458, 511, 1087
Hpy99I CGWCG 3 cut(s) 26, 144, 491
HpyAV CCTTC 3 cut(s) 354, 542, 1093
HpyCH4III ACNGT 3 cut(s) 22, 632, 747
HpyCH4V TGCA 7 cut(s) 526, 537, 564, 664, 816, 923, 1039
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 549
HpyF3I CTNAG 4 cut(s) 178, 435, 756, 1070
Hsp92I GRCGYC 1 cut(s) 552
Hsp92II CATG 5 cut(s) 514, 526, 841, 1045, 1180
HspAI GCGC 3 cut(s) 322, 552, 570
KasI GGCGCC 1 cut(s) 551
Ksp22I TGATCA 1 cut(s) 1135
Kzo9I GATC 7 cut(s) 51, 81, 340, 904, 1027, 1053, 1135
LguI GCTCTTC 2 cut(s) 30, 933
LmnI GCTCC 2 cut(s) 540, 1114
Lsp1109I GCAGC 3 cut(s) 27, 648, 935
LweI GCATC 1 cut(s) 463
MaeI CTAG 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 881
MalI GATC 7 cut(s) 53, 83, 342, 906, 1029, 1055, 1137
MboI GATC 7 cut(s) 51, 81, 340, 904, 1027, 1053, 1135
MboII GAAGA 5 cut(s) 47, 178, 463, 920, 1011
MflI RGATCY 2 cut(s) 51, 1027
MhlI GDGCHC 1 cut(s) 277
MluCI AATT 2 cut(s) 266, 635
Mly113I GGCGCC 1 cut(s) 552
MlyI GAGTC 1 cut(s) 748
MmeI TCCRAC 2 cut(s) 150, 510
MroXI GAANNNNTTC 1 cut(s) 383
MseI TTAA 2 cut(s) 45, 909
MslI CAYNNNNRTG 3 cut(s) 303, 842, 888
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 3 cut(s) 271, 441, 933
MspR9I CCNGG 4 cut(s) 272, 725, 933, 1047
Mva1269I GAATGC 1 cut(s) 952
MvaI CCWGG 2 cut(s) 725, 1047
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 46, 549
NarI GGCGCC 1 cut(s) 552
NciI CCSGG 2 cut(s) 272, 933
NdeII GATC 7 cut(s) 51, 81, 340, 904, 1027, 1053, 1135
NlaIII CATG 5 cut(s) 514, 526, 841, 1045, 1180
NlaIV GGNNCC 3 cut(s) 66, 500, 553
NmuCI GTSAC 1 cut(s) 881
NruI TCGCGA 1 cut(s) 26
NspI RCATGY 1 cut(s) 1045
OliI CACNNNNGTG 1 cut(s) 888
PagI TCATGA 1 cut(s) 510
PciSI GCTCTTC 2 cut(s) 30, 933
PcsI WCGNNNNNNNCGW 2 cut(s) 30, 886
PctI GAATGC 1 cut(s) 952
PdmI GAANNNNTTC 1 cut(s) 383
PfeI GAWTC 3 cut(s) 209, 308, 1016
PflMI CCANNNNNTGG 1 cut(s) 108
PfoI TCCNGGA 1 cut(s) 931
PkrI GCNGC 4 cut(s) 42, 146, 663, 925
PleI GAGTC 1 cut(s) 748
PluTI GGCGCC 1 cut(s) 555
PpsI GAGTC 1 cut(s) 748
PsiI TTATAA 2 cut(s) 399, 1065
Psp6I CCWGG 2 cut(s) 723, 1045
PspGI CCWGG 2 cut(s) 723, 1045
PspN4I GGNNCC 3 cut(s) 66, 500, 553
PstI CTGCAG 1 cut(s) 539
PsuI RGATCY 2 cut(s) 51, 1027
RruI TCGCGA 1 cut(s) 26
RsaI GTAC 1 cut(s) 791
RsaNI GTAC 1 cut(s) 790
RseI CAYNNNNRTG 3 cut(s) 303, 842, 888
SapI GCTCTTC 2 cut(s) 30, 933
SaqAI TTAA 2 cut(s) 45, 909
SatI GCNGC 4 cut(s) 41, 145, 662, 924
Sau3AI GATC 7 cut(s) 51, 81, 340, 904, 1027, 1053, 1135
SchI GAGTC 1 cut(s) 748
ScrFI CCNGG 4 cut(s) 272, 725, 933, 1047
SduI GDGCHC 1 cut(s) 277
SfaNI GCATC 1 cut(s) 463
SfcI CTRYAG 1 cut(s) 535
SfoI GGCGCC 1 cut(s) 553
SmiMI CAYNNNNRTG 3 cut(s) 303, 842, 888
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 2 cut(s) 266, 635
SsiI CCGC 1 cut(s) 145
SspDI GGCGCC 1 cut(s) 551
SspI AATATT 1 cut(s) 827
SspMI CTAG 1 cut(s) 192
StyD4I CCNGG 4 cut(s) 270, 723, 931, 1045
TaaI ACNGT 3 cut(s) 22, 632, 747
TaqI TCGA 3 cut(s) 33, 459, 672
TasI AATT 2 cut(s) 266, 635
TatI WGTACW 1 cut(s) 789
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 3 cut(s) 209, 308, 1016
Tru1I TTAA 2 cut(s) 45, 909
Tru9I TTAA 2 cut(s) 45, 909
TseFI GTSAC 1 cut(s) 881
TseI GCWGC 3 cut(s) 40, 661, 923
Tsp45I GTSAC 1 cut(s) 881
TspDTI ATGAA 5 cut(s) 420, 499, 527, 566, 628
TspGWI ACGGA 3 cut(s) 74, 128, 866
Van91I CCANNNNNTGG 1 cut(s) 108
Vha464I CTTAAG 1 cut(s) 44
XapI RAATTY 2 cut(s) 266, 635
XceI RCATGY 1 cut(s) 1045
XcmI CCANNNNNNNNNTGG 1 cut(s) 209
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.