Rorug06G0011400

F-box protein At5g07610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000449
Physical Location & Seq
Forward (+)
1561892 .. 1565714
3823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0011400.1

Sequence Viewer

Length: 3387 bp
ATGAGCATACAAAGAACCTCTACATCTTTCCCCCCTTCACCTCCTCAGTGGAAATATGATGTCTTTTTGAGTTTTAGAGGCGAGGATACTCGAAAGGCTTTTACAGACCACTTATACGCTGCATTGGAACATCAAGGAATCATAACTTTCAGGGATGACCCTGAACTTCAAAAAGGTAAAGCTATTTCTCCGGAACTTTTTGCTGCAATTGAAGAATCAAGATTTTCTCTCATTGTTCTGTCACAAAATTATGCATCGTCAACATGGTGCTTGGATGAGCTTGTAAGAATTCTTGAATGCATGAAAGCAAGAGAAAGTGTGTTGCCAATTTTCTATGATGTTGATCCCTCTGATGTACGAAAGCAAACAGGATGTTTTAGAGAAGCCTTCATTAATCATGAAGAAAGGTTTAGGGATGATAAAGAGAAAGTGCGAAGGTGGAGATATGCTTTAACTGAAGTGGCAACTTTCTCCGGGTGGAATTCAAAGGAATGGTATGAATCAAAGCTCATTAGAGATATTGTTGAAGTTATATGGACAAAATTGCAACCTACATTCAGCTATGCAGAAAATCTAGTTGGAATTTACTCAAGATTGCAGCCAGTCAATTTGCTTTTAGGTGTAGGGGTGGATGATGTCCGCTTCATTGGGATATGGGGAATGGGTGGGATTGGTAAGACAACTATTGTAAGAGCGGTGTATGAGAGAATCTCTCGTGAATTTGAATTTAGTTTCCCTCTTATGGATGTTAGAAGCTCCGTTGAAAAAAGTGACCTACTTAATTTACAAAAGCAGCTTCTCTCTGGGATCTTGACAAAACAGGCCAACATATCAGACCTTCATGAAGGAGCCACCATTATAAGGAGGTTGTTAGGTCAAAAAAAAGTTCTTCTCATTCTTGATAATGTGAACCATTCAAGCCATTTAAAATATTTGGCAGGAAACCAAGAGTGGTTTGGTTCAGGGAGTAGAGTTCTTATCACAACTAGAAATGAGCATTTGTTGATTGAACATGGAGTGGAGAGAAGATTGAAGGTCGAAGAATTAAATCATGATGATTCTCTTCAGCTTTTTAGCTGGAAAGCATTCAAAAGAGTTTACCCTGAAGAAGATTTTCTTGATTTGTCAGAATATGTTATAGATTATGCCAAAGGTCTTCCTTTAGCTCTTGAAGTACTGGGTTCTTTTTTGTATGGAAGAGACCTAAGTGAATGGAATAGTGCATTGACAAAACTGGGAAGAGTTTGTAACTTGGAAATTTTTGACATACTTAAAGTAAGTTATGATGGTCTGGATGATGAGGAGAAGAAAATATTCCTAGACATTGCATGCTTCTTTTGCGGAGAGGACAAAGATCGAGTAACAGAAGTACTGATGAGTTGCGATGTTTCTGCAATTATTGGATTAAAAGTTCTTACCGAAAGATCTCTCTTGACTGTTTCGCATGGAAGACTATGGATGCACGATTTGCTCCAAGAAATGGGTTGGGAAATTGTACGCCGGGAATCTACTAATGAGCCAGGCAAGCGCAGTAGGTTATGGCTTCCTGAAGACATCAAACATGTCTTGACCAAAAATAATGGAACTGAAGCAATAGAAGGCATATTTGTGGACTTAACTGAGTCAGAAGTAAAGGTGGACGTGAATCGAAAATCATTTTCAATGATGAACAAATTGAGATACCTGAAGATTAATAATGGGAACCTACCTAAGGGGCTTGAATCTCTTCCCAATAGTTTACGGCTTCTTGATTGGACGAGGTATCCGTTAAAATCTCTGCCATCACATTTCGACCCTCAAAAGCTGCTCGAACTTAGTTTGTGTCATAGCTGTATTAAACATGTTCGGATAGGAACTGAGCCTTTATACAATTTGCAAACCATTAATCTGAGTCACTCTCTGAATCTTGTCAGCACCCCAAAATTTAAAGGCATGCCATATCTCGAGTTCATAGTTCTTGAAGGTTGTATAAGATTGTATAAGGTTGATCCAGGAATTGAAGTGCTTGAAAGACTTATTGTGCTGAACTTGAAAGATTGCAAGAATCTTGTGCGTCTTCCAAGCAGTGTAAGTGGCTTAAAATCTCTCGAAGATCTCAATCTTTCTGGTTGCTCAAAGCTTGACAAATTGCCAGATGAGTTGGGTCATGTTGCTTGTTTGGAGACACTTGATGTGAGTGGATCTGGCATAAGAGAAGTGCCCTCCTCAATTGGTCTTTTGAAAAACTTGAAAGAATTATCCCTTCACGGGTGTAAAGCACAGTCACCTAAATCCTGGAATATGGTGTTCAACCCTTTTCAGTTATTGCAAAAAAGAAGTCACATTCGCGCAGAATTGTCATTGCATTGTTTATCTGGTTTGCATTCATTAACCAAACTGGATTTAAGTGACTGCAATCTTTCTGAAGAAGCAATGCCGAGTGATTTTCGATGCTTGTCTTCATTGAGGGAATTAAATCTCAGCAGAAATCAATTTGTTAGACTACCTGGGAGCATCGGCCAACTCTCTGGACTTGAAACTCTTCTCTTGAATCAGTGCCGTAAGCTCAGGATATTACCAGAGCTTCCATCTTATGTATGGGTAGACGCTAGCAACTGCATTTCATTAGATACATTGGCCAATCAAAATGGACAATACAATTCGATGCTAGAAGGAACATTTGTTAATTGTTTCAAAATGGTGGAGAATGAAAGCTGTGGGAGTATAGCACTTTCATTGCTAACACGCTATCTGAAGTTTCAACATTGGGGTTCGTCCGTACATTCTACCCGTAATTTTAGCTTTATTGGTCCTGGAAATGAAATACCAGAGTGGTACAATCACCAAAGTGTGGGGTCTTCAGTAACTGTAGAGCTGCATCCAGGTTGGTTTAGTAACAAGTGGACGGGATTCGCCTTCTGTGTCGTTTTTAGACTTCTCAACCCACTCCCGCCTTTGGTTAAGATGTCCATTATGTGCTCATGGACGGCCAATGGACAATCCTTGAGCAGCTTTGGTCTAGGGTTTGGTGAAGAGTGGGGTCTGCCTGTGTTGGATCACACTTGGTTCACCTTTTTGCCCCGTGTTGGATTCTTCGAAGAGTGGCAGGATATCTACTATCAGCTTCTAATCTCATTTCATTTCTGCCACGTGGATGCCGCAATAATAAATGAGGAAATTCTGCAAGTGAAGAACTGTGGGGTCCGTATGGTATACGAGGAAGATGTGGAGGAGCTTTGGCAAACATTAATGAAGCAAAACAATACCGGACGAGGCCTTCAACACTATGATAATGATGATGATGATGATGCAGCATCTAGTAGTGCAAGTCGGGCTCATCCAAATAGATTTAAACAACTCCACCTTGAGGGGGCAGGACCTAGTGTAAGCCCTTACATTCATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

1128

Amino Acids

128.68

Weight (kDa)

5.9

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 188 5.9e-54 TIR domain
TIR_2 PF13676 21 - 116 4.2e-15 TIR domain
NB-ARC PF00931 211 - 365 7.9e-20 NB-ARC domain
WHD_ROQ1 PF23282 433 - 503 3.7e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 667 - 757 5e-11 Leucine-rich repeat region
LRR_14 PF23598 782 - 857 2e-06 Leucine-rich repeat region
C-JID PF20160 931 - 1070 8.6e-19 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000449)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12890 FvH4_4g01370 FvH4_4g01420 FvH4_4g01430 FvH4_4g01472 FvH4_4g01490 FvH4_6g31140 FvH4_6g31150 FvH4_6g31161 FvH4_6g43941 FvH4_6g43961
rosa_chinensis RchiOBHm_Chr4g0387951 RchiOBHm_Chr4g0388001 RchiOBHm_Chr4g0388011 RchiOBHm_Chr4g0388051 RchiOBHm_Chr4g0388631 RchiOBHm_Chr4g0388641 RchiOBHm_Chr4g0388661 RchiOBHm_Chr4g0388801
rosa_laevigata RLG00000010028 RLG00000010030 RLG00000010032 RLG00000010082 RLG00000010086 RLG00000010089 RLG00000010091 RLG00000014277
rosa_multiflora Rmu_co8138914.1_g000001 Rmu_sc0000697.1_g000015 Rmu_sc0002180.1_g000030 Rmu_sc0002509.1_g000011 Rmu_sc0002509.1_g000012 Rmu_sc0002509.1_g000020 Rmu_sc0002509.1_g000021 Rmu_sc0004459.1_g000003 Rmu_sc0004459.1_g000005 Rmu_sc0006767.1_g000007 Rmu_sc0014755.1_g000003 Rmu_sc0014755.1_g000004 Rmu_sc0036097.1_g000001
rosa_roxburghii Rroxscaffold_2G00089440 Rroxscaffold_3G00220040 Rroxscaffold_5G00334500 Rroxscaffold_5G00334530 Rroxscaffold_5G00334540 Rroxscaffold_5G00335080
rosa_rugosa Rorug02G0485300 Rorug03G0314100 Rorug03G0314200 Rorug03G0314500 Rorug03G0314500 Rorug03G0314600 Rorug03G0319800 Rorug03G0319900 Rorug03G0320000 Rorug06G0011400
rosa_samantha Rh4AG019700 Rh4AG019900 Rh4AG020200 Rh4AG023900 Rh4AG024000 Rh4AG024700 Rh4BG013900 Rh4BG014100 Rh4BG014200 Rh4BG014300 Rh4BG014400 Rh4BG014500 Rh4BG014600 Rh4BG014700 Rh4BG017100 Rh4BG017400 Rh4BG017500 Rh4BG017600 Rh4BG017700 Rh4BG089200 Rh4DG014500 Rh4DG014800 Rh4DG014900 Rh4DG015400 Rh4DG017900 Rh4DG018000 Rh6BG127900 Rh6CG126300 Rh6DG113400
rosa_wichuraiana Rw4G001270 Rw4G001320 Rw4G001350 Rw4G001700 Rw4G001710 Rw4G001720 Rw4G001760 Rw6G011430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 860
AccB7I CCANNNNNTGG 2 cut(s) 1480, 2830
AccBSI CCGCTC 1 cut(s) 695
AccI GTMKAC 2 cut(s) 2583, 3192
AccII CGCG 1 cut(s) 2328
AccIII TCCGGA 1 cut(s) 190
AciI CCGC 5 cut(s) 640, 695, 1341, 2930, 3138
AclWI GGATC 5 cut(s) 338, 815, 1982, 2188, 3042
AcoI YGGCCR 3 cut(s) 2497, 2616, 2967
AcsI RAATTY 8 cut(s) 288, 481, 582, 719, 725, 1257, 1922, 3156
AcuI CTGAAG 9 cut(s) 477, 1049, 1125, 1569, 1608, 1706, 2424, 2753, 2823
AcvI CACGTG 1 cut(s) 3130
AfaI GTAC 6 cut(s) 357, 1176, 1371, 1497, 2760, 2816
AflIII ACRYGT 2 cut(s) 1561, 1840
AjiI CACGTC 1 cut(s) 1642
AjnI CCWGG 6 cut(s) 1519, 1990, 2273, 2485, 2791, 2860
AjuI GAANNNNNNNTTGG 2 cut(s) 561, 593
AleI CACNNNNGTG 1 cut(s) 2826
AloI GAACNNNNNNTCC 2 cut(s) 1395, 1427
Alw21I GWGCWC 1 cut(s) 2960
Alw26I GTCTC 2 cut(s) 1194, 2156
AlwI GGATC 5 cut(s) 338, 815, 1982, 2188, 3042
AlwNI CAGNNNCTG 1 cut(s) 2846
Ama87I CYCGRG 1 cut(s) 1943
Aor13HI TCCGGA 1 cut(s) 190
AoxI GGCC 5 cut(s) 822, 2497, 2616, 2967, 3253
ApeKI GCWGC 8 cut(s) 119, 203, 598, 793, 1804, 2854, 2988, 3290
ApoI RAATTY 8 cut(s) 288, 481, 582, 719, 725, 1257, 1922, 3156
ArsI GACNNNNNNTTYG 2 cut(s) 2302, 2334
AseI ATTAAT 4 cut(s) 393, 1692, 1884, 3227
Asp700I GAANNNNTTC 5 cut(s) 1085, 1113, 1313, 1725, 2520
AspLEI GCGC 2 cut(s) 1530, 2330
AspS9I GGNCC 3 cut(s) 2789, 3181, 3356
AsuC2I CCSGG 2 cut(s) 475, 1502
AsuHPI GGTGA 5 cut(s) 30, 2256, 2813, 3020, 3040
AsuII TTCGAA 1 cut(s) 3075
AsuNHI GCTAGC 1 cut(s) 2588
AvaI CYCGRG 1 cut(s) 1943
AvaII GGWCC 3 cut(s) 2789, 3181, 3356
AxyI CCTNAGG 1 cut(s) 1710
BaeGI GKGCMC 1 cut(s) 2202
BalI TGGCCA 1 cut(s) 2618
BanII GRGCYC 1 cut(s) 3316
BauI CACGAG 1 cut(s) 714
BbrPI CACGTG 1 cut(s) 3130
BbsI GAAGAC 6 cut(s) 1148, 1456, 1557, 2048, 2430, 2829
Bbv12I GWGCWC 1 cut(s) 2960
BbvI GCAGC 8 cut(s) 106, 190, 610, 805, 1791, 2841, 3000, 3302
BccI CCATC 3 cut(s) 1280, 1789, 2575
BceAI ACGGC 3 cut(s) 1757, 2523, 2982
BcgI CGANNNNNNTGC 2 cut(s) 1373, 1407
BciT130I CCWGG 6 cut(s) 1521, 1992, 2275, 2487, 2793, 2862
BciVI GTATCC 2 cut(s) 79, 1773
BcnI CCSGG 2 cut(s) 475, 1502
BcoDI GTCTC 2 cut(s) 1194, 2156
BfaI CTAG 9 cut(s) 575, 987, 1319, 2589, 2648, 2999, 3297, 3360, 3385
BfmI CTRYAG 1 cut(s) 2847
BfuI GTATCC 2 cut(s) 79, 1773
BglII AGATCT 2 cut(s) 1424, 2092
BisI GCNGC 9 cut(s) 120, 204, 599, 794, 1805, 2855, 2989, 3138, 3291
BlsI GCNGC 9 cut(s) 121, 205, 600, 795, 1806, 2856, 2990, 3139, 3292
BmcAI AGTACT 2 cut(s) 1176, 1371
Bme1390I CCNGG 8 cut(s) 475, 1502, 1521, 1992, 2275, 2487, 2793, 2862
Bme18I GGWCC 3 cut(s) 2789, 3181, 3356
BmeT110I CYCGRG 1 cut(s) 1943
BmgBI CACGTC 1 cut(s) 1642
BmgT120I GGNCC 3 cut(s) 2789, 3181, 3356
BmiI GGNNCC 3 cut(s) 850, 1703, 3182
BmrFI CCNGG 8 cut(s) 475, 1502, 1521, 1992, 2275, 2487, 2793, 2862
BmrI ACTGGG 2 cut(s) 1187, 1244
BmsI GCATC 9 cut(s) 263, 1449, 2420, 2502, 2634, 2866, 3124, 3277, 3302
BmtI GCTAGC 1 cut(s) 2592
BmuI ACTGGG 2 cut(s) 1187, 1244
BpiI GAAGAC 6 cut(s) 1148, 1456, 1557, 2048, 2430, 2829
BplI GAGNNNNNCTC 6 cut(s) 695, 727, 697, 729, 1882, 1914
Bpu10I CCTNAGC 1 cut(s) 2546
Bpu14I TTCGAA 1 cut(s) 3075
BpuEI CTTGAG 3 cut(s) 574, 3004, 3365
BpuMI CCSGG 2 cut(s) 475, 1502
BsaAI YACGTR 1 cut(s) 3130
BsaBI GATNNNNATC 2 cut(s) 342, 2097
BsaI GGTCTC 1 cut(s) 1194
BsaJI CCNNGG 1 cut(s) 2486
BsaWI WCCGGW 2 cut(s) 190, 3245
Bse1I ACTGG 4 cut(s) 602, 1182, 1239, 2382
Bse21I CCTNAGG 1 cut(s) 1710
Bse3DI GCAATG 4 cut(s) 1323, 2339, 2418, 2714
Bse8I GATNNNNATC 2 cut(s) 342, 2097
BseAI TCCGGA 1 cut(s) 190
BseBI CCWGG 6 cut(s) 1521, 1992, 2275, 2487, 2793, 2862
BseDI CCNNGG 1 cut(s) 2486
BseJI GATNNNNATC 2 cut(s) 342, 2097
BseMI GCAATG 4 cut(s) 1323, 2339, 2418, 2714
BseMII CTCAG 6 cut(s) 59, 1611, 1848, 1880, 2473, 2560
BseNI ACTGG 4 cut(s) 602, 1182, 1239, 2382
BseRI GAGGAG 4 cut(s) 33, 1316, 2194, 3224
BseSI GKGCMC 1 cut(s) 2202
BseXI GCAGC 8 cut(s) 106, 190, 610, 805, 1791, 2841, 3000, 3302
Bsh1236I CGCG 1 cut(s) 2328
BshFI GGCC 5 cut(s) 824, 2499, 2618, 2969, 3255
BsiHKAI GWGCWC 1 cut(s) 2960
BsiHKCI CYCGRG 1 cut(s) 1943
BsiSI CCGG 4 cut(s) 191, 474, 1501, 3246
BsmAI GTCTC 2 cut(s) 1194, 2156
BsmI GAATGC 3 cut(s) 302, 1085, 2362
BsnI GGCC 5 cut(s) 824, 2499, 2618, 2969, 3255
Bso31I GGTCTC 1 cut(s) 1194
BsoBI CYCGRG 1 cut(s) 1943
Bsp119I TTCGAA 1 cut(s) 3075
Bsp1286I GDGCHC 3 cut(s) 2202, 2960, 3316
Bsp13I TCCGGA 1 cut(s) 190
Bsp143I GATC 8 cut(s) 343, 807, 1354, 1424, 1987, 2092, 2180, 3034
BspACI CCGC 5 cut(s) 640, 695, 1341, 2930, 3138
BspANI GGCC 5 cut(s) 824, 2499, 2618, 2969, 3255
BspCNI CTCAG 6 cut(s) 58, 1612, 1849, 1881, 2472, 2559
BspEI TCCGGA 1 cut(s) 190
BspFNI CGCG 1 cut(s) 2328
BspHI TCATGA 4 cut(s) 397, 841, 1051, 3379
BspLI GGNNCC 3 cut(s) 850, 1703, 3182
BspOI GCTAGC 1 cut(s) 2592
BspPI GGATC 5 cut(s) 338, 815, 1982, 2188, 3042
BspT104I TTCGAA 1 cut(s) 3075
BspTNI GGTCTC 1 cut(s) 1194
BsrBI CCGCTC 1 cut(s) 695
BsrDI GCAATG 4 cut(s) 1323, 2339, 2418, 2714
BsrI ACTGG 4 cut(s) 602, 1182, 1239, 2382
BssECI CCNNGG 1 cut(s) 2486
BssMI GATC 8 cut(s) 343, 807, 1354, 1424, 1987, 2092, 2180, 3034
BssNAI GTATAC 1 cut(s) 3193
BssSI CACGAG 1 cut(s) 714
Bst1107I GTATAC 1 cut(s) 3193
Bst2BI CACGAG 1 cut(s) 714
Bst2UI CCWGG 6 cut(s) 1521, 1992, 2275, 2487, 2793, 2862
Bst4CI ACNGT 4 cut(s) 1438, 2262, 2848, 3176
Bst6I CTCTTC 7 cut(s) 1068, 1192, 1234, 1731, 2526, 3006, 3072
BstAPI GCANNNNNTGC 1 cut(s) 1468
BstBAI YACGTR 1 cut(s) 3130
BstBI TTCGAA 1 cut(s) 3075
BstC8I GCNNGC 4 cut(s) 1330, 1526, 1934, 2590
BstDEI CTNAG 9 cut(s) 45, 1205, 1620, 1710, 1814, 1857, 1889, 2459, 2546
BstENI CCTNNNNNAGG 1 cut(s) 1709
BstFNI CGCG 1 cut(s) 2328
BstHHI GCGC 2 cut(s) 1530, 2330
BstKTI GATC 8 cut(s) 346, 810, 1357, 1427, 1990, 2095, 2183, 3037
BstMAI GTCTC 2 cut(s) 1194, 2156
BstMBI GATC 8 cut(s) 343, 807, 1354, 1424, 1987, 2092, 2180, 3034
BstMWI GCNNNNNNNGC 4 cut(s) 1338, 1468, 1525, 3311
BstNI CCWGG 6 cut(s) 1521, 1992, 2275, 2487, 2793, 2862
BstNSI RCATGY 4 cut(s) 1332, 1565, 1844, 1936
BstSCI CCNGG 8 cut(s) 473, 1500, 1519, 1990, 2273, 2485, 2791, 2860
BstSFI CTRYAG 1 cut(s) 2847
BstSLI GKGCMC 1 cut(s) 2202
BstUI CGCG 1 cut(s) 2328
BstV1I GCAGC 8 cut(s) 106, 190, 610, 805, 1791, 2841, 3000, 3302
BstV2I GAAGAC 6 cut(s) 1148, 1456, 1557, 2048, 2430, 2829
BstX2I RGATCY 4 cut(s) 807, 1424, 2092, 2180
BstXI CCANNNNNNTGG 1 cut(s) 2507
BstYI RGATCY 4 cut(s) 807, 1424, 2092, 2180
BstZ17I GTATAC 1 cut(s) 3193
Bsu36I CCTNAGG 1 cut(s) 1710
BsuI GTATCC 2 cut(s) 79, 1773
BsuRI GGCC 5 cut(s) 824, 2499, 2618, 2969, 3255
BtgZI GCGATG 1 cut(s) 1398
BtrI CACGTC 1 cut(s) 1642
BtsI GCAGTG 1 cut(s) 2071
BtsIMutI CAGTG 3 cut(s) 53, 2071, 2540
Cac8I GCNNGC 4 cut(s) 1330, 1526, 1934, 2590
CaiI CAGNNNCTG 1 cut(s) 2846
CciI TCATGA 4 cut(s) 397, 841, 1051, 3379
CfoI GCGC 2 cut(s) 1530, 2330
Cfr13I GGNCC 3 cut(s) 2789, 3181, 3356
CseI GACGC 2 cut(s) 2042, 2594
Csp6I GTAC 6 cut(s) 356, 1175, 1370, 1496, 2759, 2815
CviQI GTAC 6 cut(s) 356, 1175, 1370, 1496, 2759, 2815
DdeI CTNAG 9 cut(s) 45, 1205, 1620, 1710, 1814, 1857, 1889, 2459, 2546
DpnI GATC 8 cut(s) 345, 809, 1356, 1426, 1989, 2094, 2182, 3036
DpnII GATC 8 cut(s) 343, 807, 1354, 1424, 1987, 2092, 2180, 3034
DraI TTTAAA 3 cut(s) 927, 1927, 3331
EaeI YGGCCR 3 cut(s) 2497, 2616, 2967
Eam1104I CTCTTC 7 cut(s) 1068, 1192, 1234, 1731, 2526, 3006, 3072
EarI CTCTTC 7 cut(s) 1068, 1192, 1234, 1731, 2526, 3006, 3072
Eco147I AGGCCT 1 cut(s) 3255
Eco24I GRGCYC 1 cut(s) 3316
Eco31I GGTCTC 1 cut(s) 1194
Eco32I GATATC 1 cut(s) 3091
Eco47I GGWCC 3 cut(s) 2789, 3181, 3356
Eco57I CTGAAG 9 cut(s) 477, 1049, 1125, 1569, 1608, 1706, 2424, 2753, 2823
Eco72I CACGTG 1 cut(s) 3130
Eco81I CCTNAGG 1 cut(s) 1710
Eco88I CYCGRG 1 cut(s) 1943
EcoNI CCTNNNNNAGG 1 cut(s) 1709
EcoO109I RGGNCCY 1 cut(s) 3356
EcoRI GAATTC 2 cut(s) 288, 481
EcoRII CCWGG 6 cut(s) 1519, 1990, 2273, 2485, 2791, 2860
EcoRV GATATC 1 cut(s) 3091
EcoT22I ATGCAT 2 cut(s) 256, 302
EcoT38I GRGCYC 1 cut(s) 3316
FalI AAGNNNNNCTT 2 cut(s) 1101, 1133
FauI CCCGC 1 cut(s) 2937
FblI GTMKAC 2 cut(s) 2583, 3192
Fnu4HI GCNGC 9 cut(s) 120, 204, 599, 794, 1805, 2855, 2989, 3138, 3291
FriOI GRGCYC 1 cut(s) 3316
Fsp4HI GCNGC 9 cut(s) 120, 204, 599, 794, 1805, 2855, 2989, 3138, 3291
FspBI CTAG 9 cut(s) 575, 987, 1319, 2589, 2648, 2999, 3297, 3360, 3385
GlaI GCGC 2 cut(s) 1529, 2329
GluI GCNGC 9 cut(s) 120, 204, 599, 794, 1805, 2855, 2989, 3138, 3291
HaeIII GGCC 5 cut(s) 824, 2499, 2618, 2969, 3255
HapII CCGG 4 cut(s) 191, 474, 1501, 3246
HgaI GACGC 2 cut(s) 2042, 2594
HhaI GCGC 2 cut(s) 1530, 2330
Hin6I GCGC 2 cut(s) 1528, 2328
HinP1I GCGC 2 cut(s) 1528, 2328
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 2117
HpaII CCGG 4 cut(s) 191, 474, 1501, 3246
HphI GGTGA 5 cut(s) 30, 2256, 2813, 3020, 3040
Hpy188I TCNGA 9 cut(s) 352, 835, 1129, 1627, 1848, 1890, 1902, 2404, 2733
HpyCH4III ACNGT 4 cut(s) 1438, 2262, 2848, 3176
HpyCH4IV ACGT 2 cut(s) 1641, 3129
HpyF10VI GCNNNNNNNGC 4 cut(s) 1338, 1468, 1525, 3311
HpyF3I CTNAG 9 cut(s) 45, 1205, 1620, 1710, 1814, 1857, 1889, 2459, 2546
HpySE526I ACGT 2 cut(s) 1641, 3129
HspAI GCGC 2 cut(s) 1528, 2328
Kpn2I TCCGGA 1 cut(s) 190
Kzo9I GATC 8 cut(s) 343, 807, 1354, 1424, 1987, 2092, 2180, 3034
LmnI GCTCC 5 cut(s) 761, 848, 1476, 2490, 3211
Lsp1109I GCAGC 8 cut(s) 106, 190, 610, 805, 1791, 2841, 3000, 3302
LweI GCATC 9 cut(s) 263, 1449, 2420, 2502, 2634, 2866, 3124, 3277, 3302
MaeI CTAG 9 cut(s) 575, 987, 1319, 2589, 2648, 2999, 3297, 3360, 3385
MaeII ACGT 2 cut(s) 1641, 3129
MalI GATC 8 cut(s) 345, 809, 1356, 1426, 1989, 2094, 2182, 3036
MbiI CCGCTC 1 cut(s) 695
MboI GATC 8 cut(s) 343, 807, 1354, 1424, 1987, 2092, 2180, 3034
MfeI CAATTG 2 cut(s) 207, 2208
MflI RGATCY 4 cut(s) 807, 1424, 2092, 2180
MhlI GDGCHC 3 cut(s) 2202, 2960, 3316
MlsI TGGCCA 1 cut(s) 2618
MluNI TGGCCA 1 cut(s) 2618
MlyI GAGTC 2 cut(s) 1631, 1900
MmeI TCCRAC 3 cut(s) 559, 3012, 3046
Mox20I TGGCCA 1 cut(s) 2618
Mph1103I ATGCAT 2 cut(s) 256, 302
MroI TCCGGA 1 cut(s) 190
MroXI GAANNNNTTC 5 cut(s) 1085, 1113, 1313, 1725, 2520
MscI TGGCCA 1 cut(s) 2618
MslI CAYNNNNRTG 3 cut(s) 1607, 2826, 3132
Msp20I TGGCCA 1 cut(s) 2618
MspI CCGG 4 cut(s) 191, 474, 1501, 3246
MspR9I CCNGG 8 cut(s) 475, 1502, 1521, 1992, 2275, 2487, 2793, 2862
MunI CAATTG 2 cut(s) 207, 2208
Mva1269I GAATGC 3 cut(s) 302, 1085, 2362
MvaI CCWGG 6 cut(s) 1521, 1992, 2275, 2487, 2793, 2862
MvnI CGCG 1 cut(s) 2328
MwoI GCNNNNNNNGC 4 cut(s) 1338, 1468, 1525, 3311
NciI CCSGG 2 cut(s) 475, 1502
NdeII GATC 8 cut(s) 343, 807, 1354, 1424, 1987, 2092, 2180, 3034
NheI GCTAGC 1 cut(s) 2588
NlaIV GGNNCC 3 cut(s) 850, 1703, 3182
NmeAIII GCCGAG 1 cut(s) 2442
NmuCI GTSAC 6 cut(s) 240, 770, 1892, 2262, 2318, 2387
NsiI ATGCAT 2 cut(s) 256, 302
NspI RCATGY 4 cut(s) 1332, 1565, 1844, 1936
NspV TTCGAA 1 cut(s) 3075
OliI CACNNNNGTG 1 cut(s) 2826
PaeI GCATGC 2 cut(s) 1332, 1936
PaeR7I CTCGAG 1 cut(s) 1943
PagI TCATGA 4 cut(s) 397, 841, 1051, 3379
PceI AGGCCT 1 cut(s) 3255
PciI ACATGT 2 cut(s) 1561, 1840
PcsI WCGNNNNNNNCGW 1 cut(s) 1763
PctI GAATGC 3 cut(s) 302, 1085, 2362
PdmI GAANNNNTTC 5 cut(s) 1085, 1113, 1313, 1725, 2520
PflMI CCANNNNNTGG 2 cut(s) 1480, 2830
PfoI TCCNGGA 3 cut(s) 1990, 2273, 2791
PkrI GCNGC 9 cut(s) 121, 205, 600, 795, 1806, 2856, 2990, 3139, 3292
PleI GAGTC 2 cut(s) 1630, 1899
PmaCI CACGTG 1 cut(s) 3130
PmlI CACGTG 1 cut(s) 3130
PpsI GAGTC 2 cut(s) 1630, 1899
Ppu21I YACGTR 1 cut(s) 3130
PpuMI RGGWCCY 1 cut(s) 3356
PscI ACATGT 2 cut(s) 1561, 1840
PshBI ATTAAT 4 cut(s) 393, 1692, 1884, 3227
PsiI TTATAA 1 cut(s) 860
Psp5II RGGWCCY 1 cut(s) 3356
Psp6I CCWGG 6 cut(s) 1519, 1990, 2273, 2485, 2791, 2860
PspCI CACGTG 1 cut(s) 3130
PspGI CCWGG 6 cut(s) 1519, 1990, 2273, 2485, 2791, 2860
PspN4I GGNNCC 3 cut(s) 850, 1703, 3182
PspPI GGNCC 3 cut(s) 2789, 3181, 3356
PspPPI RGGWCCY 1 cut(s) 3356
PstNI CAGNNNCTG 1 cut(s) 2846
PsuI RGATCY 4 cut(s) 807, 1424, 2092, 2180
RsaI GTAC 6 cut(s) 357, 1176, 1371, 1497, 2760, 2816
RsaNI GTAC 6 cut(s) 356, 1175, 1370, 1496, 2759, 2815
RseI CAYNNNNRTG 3 cut(s) 1607, 2826, 3132
SatI GCNGC 9 cut(s) 120, 204, 599, 794, 1805, 2855, 2989, 3138, 3291
Sau3AI GATC 8 cut(s) 343, 807, 1354, 1424, 1987, 2092, 2180, 3034
Sau96I GGNCC 3 cut(s) 2789, 3181, 3356
ScaI AGTACT 2 cut(s) 1176, 1371
SchI GAGTC 2 cut(s) 1631, 1900
ScrFI CCNGG 8 cut(s) 475, 1502, 1521, 1992, 2275, 2487, 2793, 2862
SduI GDGCHC 3 cut(s) 2202, 2960, 3316
SfaNI GCATC 9 cut(s) 263, 1449, 2420, 2502, 2634, 2866, 3124, 3277, 3302
SfcI CTRYAG 1 cut(s) 2847
Sfr274I CTCGAG 1 cut(s) 1943
SfuI TTCGAA 1 cut(s) 3075
SinI GGWCC 3 cut(s) 2789, 3181, 3356
SlaI CTCGAG 1 cut(s) 1943
SmiMI CAYNNNNRTG 3 cut(s) 1607, 2826, 3132
SmlI CTYRAG 4 cut(s) 589, 1943, 2983, 3344
SmoI CTYRAG 4 cut(s) 589, 1943, 2983, 3344
SphI GCATGC 2 cut(s) 1332, 1936
SseBI AGGCCT 1 cut(s) 3255
SsiI CCGC 5 cut(s) 640, 695, 1341, 2930, 3138
SspI AATATT 2 cut(s) 932, 1314
SspMI CTAG 9 cut(s) 575, 987, 1319, 2589, 2648, 2999, 3297, 3360, 3385
StuI AGGCCT 1 cut(s) 3255
StyD4I CCNGG 8 cut(s) 473, 1500, 1519, 1990, 2273, 2485, 2791, 2860
TaaI ACNGT 4 cut(s) 1438, 2262, 2848, 3176
TaiI ACGT 2 cut(s) 1644, 3132
TatI WGTACW 2 cut(s) 1174, 1369
TauI GCSGC 1 cut(s) 3140
TscAI CASTG 3 cut(s) 53, 2071, 2540
TseFI GTSAC 6 cut(s) 240, 770, 1892, 2262, 2318, 2387
TseI GCWGC 8 cut(s) 119, 203, 598, 793, 1804, 2854, 2988, 3290
Tsp45I GTSAC 6 cut(s) 240, 770, 1892, 2262, 2318, 2387
TspGWI ACGGA 4 cut(s) 748, 1755, 2746, 3173
TspRI CASTG 3 cut(s) 53, 2071, 2540
Van91I CCANNNNNTGG 2 cut(s) 1480, 2830
VpaK11BI GGWCC 3 cut(s) 2789, 3181, 3356
VspI ATTAAT 4 cut(s) 393, 1692, 1884, 3227
XagI CCTNNNNNAGG 1 cut(s) 1709
XapI RAATTY 8 cut(s) 288, 481, 582, 719, 725, 1257, 1922, 3156
XceI RCATGY 4 cut(s) 1332, 1565, 1844, 1936
XcmI CCANNNNNNNNNTGG 2 cut(s) 953, 2574
XhoI CTCGAG 1 cut(s) 1943
XmiI GTMKAC 2 cut(s) 2583, 3192
XmnI GAANNNNTTC 5 cut(s) 1085, 1113, 1313, 1725, 2520
XspI CTAG 9 cut(s) 575, 987, 1319, 2589, 2648, 2999, 3297, 3360, 3385
ZrmI AGTACT 2 cut(s) 1176, 1371
Zsp2I ATGCAT 2 cut(s) 256, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.