FvH4_6g45780

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000102
Physical Location & Seq
Reverse (-)
35052588 .. 35054736
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45780.t1

Sequence Viewer

Length: 888 bp
ATGGCGGGGTTAGTGTTACCAGAGTTGGCAGCTAGCAGTGGCAAGAAGATGAAGTGTGTGTGCCTGAATTTACTTCTTGTGCTGGTTCTCTCCGGTGCCGCTATGTCGCAGAACATCACTACTACCTTGCCTGGTCACCCTGGTACACTCCCATTCAGTCTTGAAACTGGTTATGTGAGTGTGGGTGATCTGGATCAATTGCAGTTCTTTTACTACTTCATTGAGTCACAAAGGGACCCTGTAAAGGACCCTCTCTTGCTTTGGCTCACCGGAGGCCCTGGTTGTTCTGGTTTCTCTGGCCTTGTTTATGAAATTGGTCCATTAACTTTCGACTATATAGCCTTCAACGGTAGCTTTCCTACTTTCGTGGACAACCCATTTTCATGGACACAGGTTGCCAACATTATCTTCATAGATGCACCAGTCGGCACTGGTTTCTCCTACTCAACTGCACAGGAAGGTTACTATAGCAATGACACTGAATCAGCCGAAGCCGTGTACCAATTTCTACGGAAGTGGATGCTCAAACATCCCAAGTTCCGCTACAATCCACTCTACATTGCTGGAGATTCATATTCAGGCATCATTGTTCCTCAAGTCACTCTTAAAGTATCAGATGGTAATCGTAATGGAGTCAGACCACCCATGAAGCTCCAGGGATATCTACTTGGAAATCCTGTAACAGAGTTACACAGGGATGAGAATTCAAGAATAGAATTTTACCACCGCGTCACACTTATTTCTAGTGAACTTTATGCGCCAAAGTATGCTTGTTGTCGTTTACATTCGCAGCACCGCTACAGGTTGGCCGACCGGCGAACATGGATTGCAGTCGGCCGTGGATTTAATAATGTACACTTCACCATCTGTGGCCTGAGCATCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

296

Amino Acids

32.93

Weight (kDa)

7.63

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 44 - 260 3.4e-74 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000102)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33540 AT1G33540 AT1G73270 AT1G73270 AT1G73280 AT1G73280 AT1G73280 AT1G73280 AT1G73290 AT1G73290 AT1G73290 AT1G73300 AT1G73300 AT1G73310 AT1G73310 AT1G73310 AT2G22920 AT2G22920 AT2G22920 AT2G22920 AT2G22960 AT2G22970 AT2G22970 AT2G22970 AT2G22970 AT2G22980 AT2G22980 AT2G22980 AT2G22980 AT2G22980 AT2G22990 AT2G22990 AT2G22990 AT2G22990 AT2G22990 AT2G22990 AT2G23000 AT2G23000 AT2G23010 AT2G23010 AT3G10450 AT3G10450 AT3G10450 AT3G10450 AT3G12203 AT3G12203 AT3G12203 AT3G12220 AT3G12220 AT3G12220 AT3G12230 AT3G12240 AT5G09640 AT5G36180 AT5G36180 AT5G36180 AT5G36180 AT5G36180
fragaria_vesca FvH4_1g17970 FvH4_1g17970 FvH4_1g17970 FvH4_1g17970 FvH4_1g17970 FvH4_2g03550 FvH4_2g03550 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03570 FvH4_2g03570 FvH4_2g03570 FvH4_2g32860 FvH4_2g32860 FvH4_2g32860 FvH4_6g45780
malus_domestica MD05G1102900.v1.1 MD05G1103100.v1.1 MD05G1103400.v1.1 MD05G1103600.v1.1 MD15G1067200.v1.1 MD15G1299100.v1.1 MD15G1299200.v1.1
prunus_persica Prupe.6G218600_v2.0.a1 Prupe.6G218800_v2.0.a1 Prupe.8G129900_v2.0.a1 Prupe.8G130000_v2.0.a1 Prupe.8G135600_v2.0.a1 Prupe.8G135600_v2.0.a1 Prupe.8G147500_v2.0.a1 Prupe.8G147500_v2.0.a1 Prupe.8G147600_v2.0.a1 Prupe.8G147700_v2.0.a1 Prupe.8G147800_v2.0.a1 Prupe.8G147900_v2.0.a1 Prupe.8G147900_v2.0.a1 Prupe.8G148000_v2.0.a1 Prupe.8G148000_v2.0.a1 Prupe.8G148000_v2.0.a1 Prupe.8G148300_v2.0.a1
pyrus_communis pycom05g10060 pycom08g20430 pycom10g09380 pycom15g06370
rosa_chinensis RchiOBHm_Chr2g0108421 RchiOBHm_Chr2g0155051 RchiOBHm_Chr2g0176791 RchiOBHm_Chr5g0035721 RchiOBHm_Chr5g0035731 RchiOBHm_Chr5g0043261 RchiOBHm_Chr6g0249011 RchiOBHm_Chr6g0249021 RchiOBHm_Chr6g0249041 RchiOBHm_Chr6g0249051 RchiOBHm_Chr6g0249071 RchiOBHm_Chr6g0249091 RchiOBHm_Chr6g0249111 RchiOBHm_Chr6g0249131 RchiOBHm_Chr6g0249151 RchiOBHm_Chr6g0252461 RchiOBHm_Chr6g0252481 RchiOBHm_Chr6g0252491
rosa_laevigata RLG00000001434 RLG00000015094 RLG00000015095 RLG00000015096 RLG00000015099 RLG00000015101 RLG00000015103 RLG00000017668
rosa_multiflora Rmu_co7960301.1_g000001 Rmu_co8316227.1_g000001 Rmu_co8338197.1_g000001 Rmu_co8348961.1_g000001 Rmu_sc0000290.1_g000001 Rmu_sc0000290.1_g000008 Rmu_sc0001762.1_g000019 Rmu_sc0001762.1_g000023 Rmu_sc0002016.1_g000008 Rmu_sc0002016.1_g000013 Rmu_sc0002016.1_g000016 Rmu_sc0002372.1_g000025 Rmu_sc0003692.1_g000002 Rmu_sc0003692.1_g000012 Rmu_sc0003780.1_g000008 Rmu_sc0003829.1_g000040 Rmu_sc0004823.1_g000035 Rmu_sc0004832.1_g000025 Rmu_sc0006782.1_g000011 Rmu_sc0006782.1_g000015 Rmu_sc0007382.1_g000001 Rmu_sc0009208.1_g000009 Rmu_sc0017474.1_g000001 Rmu_sc0026593.1_g000002 Rmu_sc0028609.1_g000001 Rmu_ssc0000007.1_g000002
rosa_roxburghii Rroxscaffold_1G00023310 Rroxscaffold_2G00135160 Rroxscaffold_2G00135410 Rroxscaffold_7G00212680 Rroxscaffold_7G00212690 Rroxscaffold_7G00212710 Rroxscaffold_7G00212730 Rroxscaffold_7G00212740 Rroxscaffold_7G00212770 Rroxscaffold_7G00212780
rosa_rugosa Rorug02G0152900 Rorug02G0153000 Rorug02G0153100 Rorug02G0153200 Rorug02G0153300 Rorug03G0171900 Rorug04G0031100 Rorug04G0031100 Rorug04G0031100 Rorug05G0033600 Rorug05G0033700 Rorug05G0033700 Rorug05G0534900 Rorug05G0534900 Rorug05G0535000 Rorug05G0535100 Rorug05G0535200 Rorug05G0535300 Rorug05G0535400.1 Rorug05G0535500.1 Rorug05G0535600 Rorug05G0535700 Rorug05G0535900 Rorug05G0536000.1 Rorug05G0536100.1 Rorug05G0536200 Rorug05G0536200 Rorug05G0536300 Rorug06G0002300
rosa_samantha Rh1AG086800 Rh1BG137200 Rh2AG201800 Rh2BG214700 Rh2CG205900 Rh2CG650900 Rh2DG209100 Rh2DG683600 Rh2DG701600 Rh4DG048600 Rh5BG257100 Rh5BG296800 Rh5DG266200 Rh5DG306500 Rh6AG052300 Rh6AG052500 Rh6BG009400 Rh6BG045800 Rh6BG046000 Rh6BG046100 Rh6BG046300 Rh6BG046400 Rh6BG046500 Rh6CG044400 Rh6CG044500 Rh6CG044600 Rh6CG044900 Rh6CG045100 Rh6CG045200 Rh6CG178800 Rh6DG039600 Rh6DG039800 Rh6DG040100 Rh6DG040600 Rh6DG040800 Rh6DG040900 Rh7AG465600 Rh7CG509300 Rh7DG476900
rosa_wichuraiana Rw0G003830 Rw1G008040 Rw2G016060 Rw2G016130 Rw2G053970 Rw4G021320 Rw6G004520 Rw6G004560 Rw6G004640 Rw6G004660 Rw6G004670 Rw6G004680 Rw6G004700 Rw6G004720 Rw6G004730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 95
AccII CGCG 1 cut(s) 729
AciI CCGC 5 cut(s) 5, 99, 541, 727, 796
AclWI GGATC 1 cut(s) 201
AcoI YGGCCR 2 cut(s) 807, 835
AcsI RAATTY 3 cut(s) 67, 703, 716
AfaI GTAC 3 cut(s) 145, 500, 855
AfiI CCNNNNNNNGG 1 cut(s) 244
AgsI TTSAA 3 cut(s) 164, 346, 708
AjnI CCWGG 4 cut(s) 130, 139, 277, 654
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 3 cut(s) 32, 354, 652
AluI AGCT 3 cut(s) 32, 354, 652
AlwI GGATC 1 cut(s) 201
AoxI GGCC 5 cut(s) 274, 298, 807, 835, 871
ApeKI GCWGC 2 cut(s) 29, 790
ApoI RAATTY 3 cut(s) 67, 703, 716
AspLEI GCGC 1 cut(s) 760
AspS9I GGNCC 4 cut(s) 235, 247, 275, 317
AsuHPI GGTGA 4 cut(s) 128, 197, 259, 853
AsuNHI GCTAGC 1 cut(s) 32
AvaII GGWCC 3 cut(s) 235, 247, 317
BanI GGYRCC 1 cut(s) 95
BbvI GCAGC 2 cut(s) 41, 802
BccI CCATC 2 cut(s) 611, 872
BceAI ACGGC 2 cut(s) 479, 822
BciT130I CCWGG 4 cut(s) 132, 141, 279, 656
BfaI CTAG 2 cut(s) 33, 744
BfmI CTRYAG 2 cut(s) 466, 799
BisI GCNGC 3 cut(s) 30, 99, 791
BlsI GCNGC 3 cut(s) 31, 100, 792
Bme1390I CCNGG 4 cut(s) 132, 141, 279, 656
Bme18I GGWCC 3 cut(s) 235, 247, 317
BmgT120I GGNCC 4 cut(s) 235, 247, 275, 317
BmiI GGNNCC 4 cut(s) 97, 236, 237, 249
BmrFI CCNGG 4 cut(s) 132, 141, 279, 656
BmsI GCATC 3 cut(s) 406, 510, 591
BmtI GCTAGC 1 cut(s) 36
BpmI CTGGAG 2 cut(s) 585, 638
Bpu10I CCTNAGC 1 cut(s) 875
BpuEI CTTGAG 1 cut(s) 579
BsaBI GATNNNNATC 2 cut(s) 192, 621
BsaJI CCNNGG 4 cut(s) 139, 277, 655, 838
BsaWI WCCGGW 2 cut(s) 92, 269
Bsc4I CCNNNNNNNGG 1 cut(s) 244
Bse118I RCCGGY 1 cut(s) 813
Bse1I ACTGG 3 cut(s) 172, 422, 436
Bse3DI GCAATG 2 cut(s) 478, 558
Bse8I GATNNNNATC 2 cut(s) 192, 621
BseBI CCWGG 4 cut(s) 132, 141, 279, 656
BseDI CCNNGG 4 cut(s) 139, 277, 655, 838
BseGI GGATG 3 cut(s) 525, 529, 703
BseJI GATNNNNATC 2 cut(s) 192, 621
BseLI CCNNNNNNNGG 1 cut(s) 244
BseMI GCAATG 2 cut(s) 478, 558
BseMII CTCAG 1 cut(s) 866
BseNI ACTGG 3 cut(s) 172, 422, 436
BseX3I CGGCCG 1 cut(s) 835
BseXI GCAGC 2 cut(s) 41, 802
BsgI GTGCAG 1 cut(s) 435
Bsh1236I CGCG 1 cut(s) 729
Bsh1285I CGRYCG 2 cut(s) 814, 838
BshFI GGCC 5 cut(s) 276, 300, 809, 837, 873
BshNI GGYRCC 1 cut(s) 95
BsiEI CGRYCG 2 cut(s) 814, 838
BsiSI CCGG 3 cut(s) 93, 270, 814
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 5 cut(s) 276, 300, 809, 837, 873
Bsp1407I TGTACA 1 cut(s) 853
Bsp143I GATC 2 cut(s) 187, 193
BspACI CCGC 5 cut(s) 5, 99, 541, 727, 796
BspANI GGCC 5 cut(s) 276, 300, 809, 837, 873
BspCNI CTCAG 1 cut(s) 867
BspFNI CGCG 1 cut(s) 729
BspLI GGNNCC 4 cut(s) 97, 236, 237, 249
BspOI GCTAGC 1 cut(s) 36
BspPI GGATC 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 95
BsrDI GCAATG 2 cut(s) 478, 558
BsrFI RCCGGY 1 cut(s) 813
BsrGI TGTACA 1 cut(s) 853
BsrI ACTGG 3 cut(s) 172, 422, 436
BssAI RCCGGY 1 cut(s) 813
BssECI CCNNGG 4 cut(s) 139, 277, 655, 838
BssMI GATC 2 cut(s) 187, 193
Bst2UI CCWGG 4 cut(s) 132, 141, 279, 656
Bst4CI ACNGT 1 cut(s) 350
BstAUI TGTACA 1 cut(s) 853
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 1 cut(s) 875
BstDSI CCRYGG 1 cut(s) 838
BstEII GGTNACC 1 cut(s) 134
BstF5I GGATG 3 cut(s) 525, 529, 703
BstFNI CGCG 1 cut(s) 729
BstHHI GCGC 1 cut(s) 760
BstKTI GATC 2 cut(s) 190, 196
BstMBI GATC 2 cut(s) 187, 193
BstMCI CGRYCG 2 cut(s) 814, 838
BstNI CCWGG 4 cut(s) 132, 141, 279, 656
BstPI GGTNACC 1 cut(s) 134
BstSCI CCNGG 4 cut(s) 130, 139, 277, 654
BstSFI CTRYAG 2 cut(s) 466, 799
BstUI CGCG 1 cut(s) 729
BstV1I GCAGC 2 cut(s) 41, 802
BstXI CCANNNNNNTGG 1 cut(s) 384
BstZI CGGCCG 1 cut(s) 835
BsuRI GGCC 5 cut(s) 276, 300, 809, 837, 873
BtgI CCRYGG 1 cut(s) 838
BtsCI GGATG 3 cut(s) 525, 529, 703
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 3 cut(s) 43, 429, 477
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 1 cut(s) 760
Cfr10I RCCGGY 1 cut(s) 813
Cfr13I GGNCC 4 cut(s) 235, 247, 275, 317
CseI GACGC 1 cut(s) 718
Csp6I GTAC 3 cut(s) 144, 499, 854
CviAII CATG 3 cut(s) 384, 646, 822
CviQI GTAC 3 cut(s) 144, 499, 854
DdeI CTNAG 1 cut(s) 875
DpnI GATC 2 cut(s) 189, 195
DpnII GATC 2 cut(s) 187, 193
EaeI YGGCCR 2 cut(s) 807, 835
EagI CGGCCG 1 cut(s) 835
EclXI CGGCCG 1 cut(s) 835
Eco32I GATATC 1 cut(s) 662
Eco47I GGWCC 3 cut(s) 235, 247, 317
Eco52I CGGCCG 1 cut(s) 835
Eco91I GGTNACC 1 cut(s) 134
EcoO109I RGGNCCY 3 cut(s) 235, 247, 275
EcoO65I GGTNACC 1 cut(s) 134
EcoRI GAATTC 1 cut(s) 703
EcoRII CCWGG 4 cut(s) 130, 139, 277, 654
EcoRV GATATC 1 cut(s) 662
FaeI CATG 3 cut(s) 387, 649, 825
FalI AAGNNNNNCTT 2 cut(s) 588, 620
FaqI GGGAC 1 cut(s) 248
FatI CATG 3 cut(s) 383, 645, 821
Fnu4HI GCNGC 3 cut(s) 30, 99, 791
FokI GGATG 3 cut(s) 516, 532, 710
Fsp4HI GCNGC 3 cut(s) 30, 99, 791
FspBI CTAG 2 cut(s) 33, 744
GlaI GCGC 1 cut(s) 759
GluI GCNGC 3 cut(s) 30, 99, 791
GsuI CTGGAG 2 cut(s) 585, 638
HaeIII GGCC 5 cut(s) 276, 300, 809, 837, 873
HapII CCGG 3 cut(s) 93, 270, 814
HgaI GACGC 1 cut(s) 718
HhaI GCGC 1 cut(s) 760
Hin1II CATG 3 cut(s) 387, 649, 825
Hin6I GCGC 1 cut(s) 758
HinP1I GCGC 1 cut(s) 758
HinfI GANTC 4 cut(s) 224, 482, 569, 633
HpaII CCGG 3 cut(s) 93, 270, 814
HphI GGTGA 4 cut(s) 128, 197, 259, 853
Hpy166II GTNNAC 6 cut(s) 146, 370, 499, 749, 782, 856
Hpy188I TCNGA 2 cut(s) 616, 638
Hpy188III TCNNGA 3 cut(s) 161, 191, 708
Hpy8I GTNNAC 6 cut(s) 146, 370, 499, 749, 782, 856
HpyAV CCTTC 2 cut(s) 352, 452
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4V TGCA 4 cut(s) 202, 419, 452, 830
HpyF3I CTNAG 1 cut(s) 875
Hsp92II CATG 3 cut(s) 387, 649, 825
HspAI GCGC 1 cut(s) 758
KflI GGGWCCC 1 cut(s) 235
Kzo9I GATC 2 cut(s) 187, 193
LmnI GCTCC 1 cut(s) 657
Lsp1109I GCAGC 2 cut(s) 41, 802
LweI GCATC 3 cut(s) 406, 510, 591
MaeI CTAG 2 cut(s) 33, 744
MaeIII GTNAC 8 cut(s) 15, 134, 225, 461, 598, 679, 687, 730
MalI GATC 2 cut(s) 189, 195
MboI GATC 2 cut(s) 187, 193
MboII GAAGA 2 cut(s) 58, 400
MfeI CAATTG 1 cut(s) 197
MluCI AATT 6 cut(s) 67, 197, 312, 503, 703, 716
MlyI GAGTC 2 cut(s) 233, 642
MnlI CCTC 3 cut(s) 261, 266, 603
MseI TTAA 3 cut(s) 323, 606, 846
MslI CAYNNNNRTG 2 cut(s) 382, 696
MspI CCGG 3 cut(s) 93, 270, 814
MspR9I CCNGG 4 cut(s) 132, 141, 279, 656
MunI CAATTG 1 cut(s) 197
MvaI CCWGG 4 cut(s) 132, 141, 279, 656
MvnI CGCG 1 cut(s) 729
NdeII GATC 2 cut(s) 187, 193
NheI GCTAGC 1 cut(s) 32
NlaIII CATG 3 cut(s) 387, 649, 825
NlaIV GGNNCC 4 cut(s) 97, 236, 237, 249
NmuCI GTSAC 4 cut(s) 134, 225, 598, 730
PfeI GAWTC 2 cut(s) 482, 569
PkrI GCNGC 3 cut(s) 31, 100, 792
PleI GAGTC 2 cut(s) 232, 641
PpsI GAGTC 2 cut(s) 232, 641
PpuMI RGGWCCY 2 cut(s) 235, 247
Psp5II RGGWCCY 2 cut(s) 235, 247
Psp6I CCWGG 4 cut(s) 130, 139, 277, 654
PspEI GGTNACC 1 cut(s) 134
PspGI CCWGG 4 cut(s) 130, 139, 277, 654
PspN4I GGNNCC 4 cut(s) 97, 236, 237, 249
PspPI GGNCC 4 cut(s) 235, 247, 275, 317
PspPPI RGGWCCY 2 cut(s) 235, 247
RsaI GTAC 3 cut(s) 145, 500, 855
RsaNI GTAC 3 cut(s) 144, 499, 854
RseI CAYNNNNRTG 2 cut(s) 382, 696
SaqAI TTAA 3 cut(s) 323, 606, 846
SatI GCNGC 3 cut(s) 30, 99, 791
Sau3AI GATC 2 cut(s) 187, 193
Sau96I GGNCC 4 cut(s) 235, 247, 275, 317
SchI GAGTC 2 cut(s) 233, 642
ScrFI CCNGG 4 cut(s) 132, 141, 279, 656
SetI ASST 7 cut(s) 34, 128, 356, 396, 463, 654, 806
SfaNI GCATC 3 cut(s) 406, 510, 591
SfcI CTRYAG 2 cut(s) 466, 799
SinI GGWCC 3 cut(s) 235, 247, 317
SmiMI CAYNNNNRTG 2 cut(s) 382, 696
SmlI CTYRAG 1 cut(s) 594
SmoI CTYRAG 1 cut(s) 594
Sse9I AATT 6 cut(s) 67, 197, 312, 503, 703, 716
SsiI CCGC 5 cut(s) 5, 99, 541, 727, 796
SspMI CTAG 2 cut(s) 33, 744
StyD4I CCNGG 4 cut(s) 130, 139, 277, 654
TaaI ACNGT 1 cut(s) 350
TaqI TCGA 1 cut(s) 330
TasI AATT 6 cut(s) 67, 197, 312, 503, 703, 716
TatI WGTACW 1 cut(s) 853
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 2 cut(s) 482, 569
Tru1I TTAA 3 cut(s) 323, 606, 846
Tru9I TTAA 3 cut(s) 323, 606, 846
TscAI CASTG 3 cut(s) 43, 436, 484
TseFI GTSAC 4 cut(s) 134, 225, 598, 730
TseI GCWGC 2 cut(s) 29, 790
Tsp45I GTSAC 4 cut(s) 134, 225, 598, 730
TspDTI ATGAA 7 cut(s) 65, 208, 324, 372, 400, 561, 662
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 3 cut(s) 43, 436, 484
VpaK11BI GGWCC 3 cut(s) 235, 247, 317
XapI RAATTY 3 cut(s) 67, 703, 716
XspI CTAG 2 cut(s) 33, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.