Rroxscaffold_7G00212770

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000102
Physical Location & Seq
Reverse (-)
63259119 .. 63274468
15350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00212770.1

Sequence Viewer

Length: 1047 bp
ATGCCCCGTGGGCACAGGGTTCTCATATACAAACAGTTGGGAAGAATACCCCACTCGAATGACACGATATCATCGGCACAAACATATGAGTTTCTTAGAAAGTGGCTTGTAGATCACCCGAGGTTCCTCTATAATCCACTATACGTTGGTGGAGATTCCTATGGAGGCAAGGTTGTTCCGATAATAGTTCAAGAAATATCTAATGGTAATGAAGATGGACTTGAACCACAAATGAATCTCAAAGGATATGTGCTTGGCAACCCTGTGACAGATCCAGACAGGGACGAGAATTCCCGAGTACTTTTTGCTTACCTAAAAGCTCTCATTGATCATGGACTATACCAGTCAACTAAAAGAAATTGCAAAGGCGAGTATGTGAATGTGGATCCGAACAATGCATTATGTGTGTACGATCTTGAACGTGTCAACGAGTGCATCGAAGACATAAACCTTCAACAAATATTGGAACCTAAATGTAATCCCCAAGCCCCAAAACCAATAGACAGAAAGTGGCATCCGCAAGAATTCAGTAAAAAGGATCCTATTGATCTCCTCCTTCCATCATCTGAATTAGTTTCTATGCCATGGTGTCGGGCTACCAATTATATATTATCTCAAATTTGGGCGAACGATAAAACTGTTCAATCTGCTCTTCACGTTGTACAGCAGGGAAGCATCAAGGAATGGGTGAGATGTAATGACAGCTTAACTAGTTCATATGTAGAAGATGTTTCTTCTACTGTTGGTTATCACCAAAACCTCACAACAAAAGGCTATAGAGTTCTGATTTATAGTGGTGATCATGACATGAGTATTCCATATGTGGGCACTACTACCTGGATAGAATCTCTGAACTTGACTCTGGACAGTCAATGGAATCCTTGGTTCGTTAATGCACAAATTGCAGGATACAAAGTGAAGTATACATTGGGAAAATATGAGTTGACATACGCAACCGTAAAGGGAGCCGGTCACACAGCTCCAGAGTTCAATCCTAAAGAATGTTTTGCTATGATCAGTAGATGGTTCGCAGCCTACCCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S10

Protein Analysis

348

Amino Acids

39.69

Weight (kDa)

6.15

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 19 - 344 3.2e-78 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000102)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G33540 AT1G33540 AT1G73270 AT1G73270 AT1G73280 AT1G73280 AT1G73280 AT1G73280 AT1G73290 AT1G73290 AT1G73290 AT1G73300 AT1G73300 AT1G73310 AT1G73310 AT1G73310 AT2G22920 AT2G22920 AT2G22920 AT2G22920 AT2G22960 AT2G22970 AT2G22970 AT2G22970 AT2G22970 AT2G22980 AT2G22980 AT2G22980 AT2G22980 AT2G22980 AT2G22990 AT2G22990 AT2G22990 AT2G22990 AT2G22990 AT2G22990 AT2G23000 AT2G23000 AT2G23010 AT2G23010 AT3G10450 AT3G10450 AT3G10450 AT3G10450 AT3G12203 AT3G12203 AT3G12203 AT3G12220 AT3G12220 AT3G12220 AT3G12230 AT3G12240 AT5G09640 AT5G36180 AT5G36180 AT5G36180 AT5G36180 AT5G36180
fragaria_vesca FvH4_1g17970 FvH4_1g17970 FvH4_1g17970 FvH4_1g17970 FvH4_1g17970 FvH4_2g03550 FvH4_2g03550 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03560 FvH4_2g03570 FvH4_2g03570 FvH4_2g03570 FvH4_2g32860 FvH4_2g32860 FvH4_2g32860 FvH4_6g45780
malus_domestica MD05G1102900.v1.1 MD05G1103100.v1.1 MD05G1103400.v1.1 MD05G1103600.v1.1 MD15G1067200.v1.1 MD15G1299100.v1.1 MD15G1299200.v1.1
prunus_persica Prupe.6G218600_v2.0.a1 Prupe.6G218800_v2.0.a1 Prupe.8G129900_v2.0.a1 Prupe.8G130000_v2.0.a1 Prupe.8G135600_v2.0.a1 Prupe.8G135600_v2.0.a1 Prupe.8G147500_v2.0.a1 Prupe.8G147500_v2.0.a1 Prupe.8G147600_v2.0.a1 Prupe.8G147700_v2.0.a1 Prupe.8G147800_v2.0.a1 Prupe.8G147900_v2.0.a1 Prupe.8G147900_v2.0.a1 Prupe.8G148000_v2.0.a1 Prupe.8G148000_v2.0.a1 Prupe.8G148000_v2.0.a1 Prupe.8G148300_v2.0.a1
pyrus_communis pycom05g10060 pycom08g20430 pycom10g09380 pycom15g06370
rosa_chinensis RchiOBHm_Chr2g0108421 RchiOBHm_Chr2g0155051 RchiOBHm_Chr2g0176791 RchiOBHm_Chr5g0035721 RchiOBHm_Chr5g0035731 RchiOBHm_Chr5g0043261 RchiOBHm_Chr6g0249011 RchiOBHm_Chr6g0249021 RchiOBHm_Chr6g0249041 RchiOBHm_Chr6g0249051 RchiOBHm_Chr6g0249071 RchiOBHm_Chr6g0249091 RchiOBHm_Chr6g0249111 RchiOBHm_Chr6g0249131 RchiOBHm_Chr6g0249151 RchiOBHm_Chr6g0252461 RchiOBHm_Chr6g0252481 RchiOBHm_Chr6g0252491
rosa_laevigata RLG00000001434 RLG00000015094 RLG00000015095 RLG00000015096 RLG00000015099 RLG00000015101 RLG00000015103 RLG00000017668
rosa_multiflora Rmu_co7960301.1_g000001 Rmu_co8316227.1_g000001 Rmu_co8338197.1_g000001 Rmu_co8348961.1_g000001 Rmu_sc0000290.1_g000001 Rmu_sc0000290.1_g000008 Rmu_sc0001762.1_g000019 Rmu_sc0001762.1_g000023 Rmu_sc0002016.1_g000008 Rmu_sc0002016.1_g000013 Rmu_sc0002016.1_g000016 Rmu_sc0002372.1_g000025 Rmu_sc0003692.1_g000002 Rmu_sc0003692.1_g000012 Rmu_sc0003780.1_g000008 Rmu_sc0003829.1_g000040 Rmu_sc0004823.1_g000035 Rmu_sc0004832.1_g000025 Rmu_sc0006782.1_g000011 Rmu_sc0006782.1_g000015 Rmu_sc0007382.1_g000001 Rmu_sc0009208.1_g000009 Rmu_sc0017474.1_g000001 Rmu_sc0026593.1_g000002 Rmu_sc0028609.1_g000001 Rmu_ssc0000007.1_g000002
rosa_roxburghii Rroxscaffold_1G00023310 Rroxscaffold_2G00135160 Rroxscaffold_2G00135410 Rroxscaffold_7G00212680 Rroxscaffold_7G00212690 Rroxscaffold_7G00212710 Rroxscaffold_7G00212730 Rroxscaffold_7G00212740 Rroxscaffold_7G00212770 Rroxscaffold_7G00212780
rosa_rugosa Rorug02G0152900 Rorug02G0153000 Rorug02G0153100 Rorug02G0153200 Rorug02G0153300 Rorug03G0171900 Rorug04G0031100 Rorug04G0031100 Rorug04G0031100 Rorug05G0033600 Rorug05G0033700 Rorug05G0033700 Rorug05G0534900 Rorug05G0534900 Rorug05G0535000 Rorug05G0535100 Rorug05G0535200 Rorug05G0535300 Rorug05G0535400.1 Rorug05G0535500.1 Rorug05G0535600 Rorug05G0535700 Rorug05G0535900 Rorug05G0536000.1 Rorug05G0536100.1 Rorug05G0536200 Rorug05G0536200 Rorug05G0536300 Rorug06G0002300
rosa_samantha Rh1AG086800 Rh1BG137200 Rh2AG201800 Rh2BG214700 Rh2CG205900 Rh2CG650900 Rh2DG209100 Rh2DG683600 Rh2DG701600 Rh4DG048600 Rh5BG257100 Rh5BG296800 Rh5DG266200 Rh5DG306500 Rh6AG052300 Rh6AG052500 Rh6BG009400 Rh6BG045800 Rh6BG046000 Rh6BG046100 Rh6BG046300 Rh6BG046400 Rh6BG046500 Rh6CG044400 Rh6CG044500 Rh6CG044600 Rh6CG044900 Rh6CG045100 Rh6CG045200 Rh6CG178800 Rh6DG039600 Rh6DG039800 Rh6DG040100 Rh6DG040600 Rh6DG040800 Rh6DG040900 Rh7AG465600 Rh7CG509300 Rh7DG476900
rosa_wichuraiana Rw0G003830 Rw1G008040 Rw2G016060 Rw2G016130 Rw2G053970 Rw4G021320 Rw6G004520 Rw6G004560 Rw6G004640 Rw6G004660 Rw6G004670 Rw6G004680 Rw6G004700 Rw6G004720 Rw6G004730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 923
AciI CCGC 1 cut(s) 518
AclWI GGATC 5 cut(s) 266, 380, 393, 533, 546
AcsI RAATTY 3 cut(s) 289, 524, 618
AfaI GTAC 3 cut(s) 300, 410, 663
AfiI CCNNNNNNNGG 1 cut(s) 824
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 6 cut(s) 191, 224, 419, 455, 644, 991
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 1 cut(s) 836
AjuI GAANNNNNNNTTGG 2 cut(s) 911, 943
AluBI AGCT 3 cut(s) 320, 705, 980
AluI AGCT 3 cut(s) 320, 705, 980
AlwI GGATC 5 cut(s) 266, 380, 393, 533, 546
Ama87I CYCGRG 2 cut(s) 118, 294
ApeKI GCWGC 1 cut(s) 1031
ApoI RAATTY 3 cut(s) 289, 524, 618
AsuHPI GGTGA 4 cut(s) 107, 700, 743, 809
AvaI CYCGRG 2 cut(s) 118, 294
BaeGI GKGCMC 2 cut(s) 15, 830
BamHI GGATCC 2 cut(s) 385, 538
BbsI GAAGAC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 1043
BccI CCATC 3 cut(s) 209, 568, 1017
BciT130I CCWGG 1 cut(s) 838
BciVI GTATCC 1 cut(s) 902
BclI TGATCA 3 cut(s) 328, 799, 1014
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 1 cut(s) 711
BfmI CTRYAG 2 cut(s) 775, 1043
BfuI GTATCC 1 cut(s) 902
BglI GCCNNNNNGGC 1 cut(s) 10
BisI GCNGC 1 cut(s) 1032
BlsI GCNGC 1 cut(s) 1033
BmcAI AGTACT 1 cut(s) 300
Bme1390I CCNGG 1 cut(s) 838
BmeT110I CYCGRG 2 cut(s) 118, 294
BmiI GGNNCC 5 cut(s) 125, 387, 468, 540, 967
BmrFI CCNGG 1 cut(s) 838
BmsI GCATC 3 cut(s) 444, 523, 684
BpiI GAAGAC 1 cut(s) 447
BpmI CTGGAG 1 cut(s) 966
BsaJI CCNNGG 4 cut(s) 7, 119, 584, 881
BsaXI ACNNNNNCTCC 2 cut(s) 156, 186
Bsc4I CCNNNNNNNGG 1 cut(s) 824
Bse118I RCCGGY 1 cut(s) 968
Bse1I ACTGG 1 cut(s) 343
BseBI CCWGG 1 cut(s) 838
BseDI CCNNGG 4 cut(s) 7, 119, 584, 881
BseGI GGATG 1 cut(s) 514
BseLI CCNNNNNNNGG 1 cut(s) 824
BseNI ACTGG 1 cut(s) 343
BseRI GAGGAG 1 cut(s) 542
BseSI GKGCMC 2 cut(s) 15, 830
BseXI GCAGC 1 cut(s) 1043
BsiHKCI CYCGRG 2 cut(s) 118, 294
BsiSI CCGG 1 cut(s) 969
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 1 cut(s) 824
BsmFI GGGAC 1 cut(s) 296
BsoBI CYCGRG 2 cut(s) 118, 294
Bsp1286I GDGCHC 2 cut(s) 15, 830
Bsp1407I TGTACA 1 cut(s) 661
Bsp143I GATC 9 cut(s) 112, 271, 328, 385, 412, 538, 547, 799, 1014
Bsp19I CCATGG 1 cut(s) 584
BspACI CCGC 1 cut(s) 518
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 5 cut(s) 125, 387, 468, 540, 967
BspPI GGATC 5 cut(s) 266, 380, 393, 533, 546
BspQI GCTCTTC 1 cut(s) 657
BsrFI RCCGGY 1 cut(s) 968
BsrGI TGTACA 1 cut(s) 661
BsrI ACTGG 1 cut(s) 343
BssAI RCCGGY 1 cut(s) 968
BssECI CCNNGG 4 cut(s) 7, 119, 584, 881
BssMI GATC 9 cut(s) 112, 271, 328, 385, 412, 538, 547, 799, 1014
BssNAI GTATAC 1 cut(s) 924
BssT1I CCWWGG 2 cut(s) 584, 881
Bst1107I GTATAC 1 cut(s) 924
Bst2UI CCWGG 1 cut(s) 838
Bst4CI ACNGT 5 cut(s) 36, 640, 742, 869, 958
Bst6I CTCTTC 1 cut(s) 657
BstAPI GCANNNNNTGC 1 cut(s) 902
BstAUI TGTACA 1 cut(s) 661
BstDEI CTNAG 1 cut(s) 95
BstDSI CCRYGG 2 cut(s) 7, 584
BstF5I GGATG 1 cut(s) 514
BstKTI GATC 9 cut(s) 115, 274, 331, 388, 415, 541, 550, 802, 1017
BstMBI GATC 9 cut(s) 112, 271, 328, 385, 412, 538, 547, 799, 1014
BstMWI GCNNNNNNNGC 2 cut(s) 10, 902
BstNI CCWGG 1 cut(s) 838
BstSCI CCNGG 1 cut(s) 836
BstSFI CTRYAG 2 cut(s) 775, 1043
BstSLI GKGCMC 2 cut(s) 15, 830
BstV1I GCAGC 1 cut(s) 1043
BstV2I GAAGAC 1 cut(s) 447
BstX2I RGATCY 3 cut(s) 271, 385, 538
BstYI RGATCY 3 cut(s) 271, 385, 538
BstZ17I GTATAC 1 cut(s) 924
BsuI GTATCC 1 cut(s) 902
BtgI CCRYGG 2 cut(s) 7, 584
BtsCI GGATG 1 cut(s) 514
CciI TCATGA 1 cut(s) 802
Cfr10I RCCGGY 1 cut(s) 968
Csp6I GTAC 3 cut(s) 299, 409, 662
CviAII CATG 4 cut(s) 332, 585, 803, 808
CviJI RGCY 9 cut(s) 106, 320, 488, 596, 705, 774, 968, 980, 1034
CviKI_1 RGCY 9 cut(s) 106, 320, 488, 596, 705, 774, 968, 980, 1034
CviQI GTAC 3 cut(s) 299, 409, 662
DdeI CTNAG 1 cut(s) 95
DpnI GATC 9 cut(s) 114, 273, 330, 387, 414, 540, 549, 801, 1016
DpnII GATC 9 cut(s) 112, 271, 328, 385, 412, 538, 547, 799, 1014
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
Eco130I CCWWGG 2 cut(s) 584, 881
Eco32I GATATC 1 cut(s) 69
Eco88I CYCGRG 2 cut(s) 118, 294
EcoRI GAATTC 2 cut(s) 289, 524
EcoRII CCWGG 1 cut(s) 836
EcoRV GATATC 1 cut(s) 69
EcoT14I CCWWGG 2 cut(s) 584, 881
EcoT22I ATGCAT 1 cut(s) 400
ErhI CCWWGG 2 cut(s) 584, 881
FaeI CATG 4 cut(s) 335, 588, 806, 811
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 1 cut(s) 296
FatI CATG 4 cut(s) 331, 584, 802, 807
FauNDI CATATG 3 cut(s) 85, 718, 820
FbaI TGATCA 3 cut(s) 328, 799, 1014
FblI GTMKAC 1 cut(s) 923
Fnu4HI GCNGC 1 cut(s) 1032
FokI GGATG 1 cut(s) 501
Fsp4HI GCNGC 1 cut(s) 1032
FspBI CTAG 1 cut(s) 711
GluI GCNGC 1 cut(s) 1032
GsuI CTGGAG 1 cut(s) 966
HapII CCGG 1 cut(s) 969
Hin1II CATG 4 cut(s) 335, 588, 806, 811
HincII GTYRAC 3 cut(s) 348, 427, 945
HindII GTYRAC 3 cut(s) 348, 427, 945
HinfI GANTC 5 cut(s) 155, 235, 845, 859, 877
HpaII CCGG 1 cut(s) 969
HphI GGTGA 4 cut(s) 107, 700, 743, 809
Hpy166II GTNNAC 5 cut(s) 348, 409, 427, 924, 945
Hpy188I TCNGA 5 cut(s) 180, 390, 568, 786, 852
Hpy188III TCNNGA 7 cut(s) 191, 275, 294, 416, 803, 863, 983
Hpy8I GTNNAC 5 cut(s) 348, 409, 427, 924, 945
HpyAV CCTTC 2 cut(s) 461, 566
HpyCH4III ACNGT 5 cut(s) 36, 640, 742, 869, 958
HpyCH4IV ACGT 3 cut(s) 144, 421, 657
HpyCH4V TGCA 5 cut(s) 363, 398, 435, 896, 905
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 902
HpyF3I CTNAG 1 cut(s) 95
HpySE526I ACGT 3 cut(s) 144, 421, 657
Hsp92II CATG 4 cut(s) 335, 588, 806, 811
Ksp22I TGATCA 3 cut(s) 328, 799, 1014
Kzo9I GATC 9 cut(s) 112, 271, 328, 385, 412, 538, 547, 799, 1014
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 2 cut(s) 965, 985
Lsp1109I GCAGC 1 cut(s) 1043
LweI GCATC 3 cut(s) 444, 523, 684
MaeI CTAG 1 cut(s) 711
MaeII ACGT 3 cut(s) 144, 421, 657
MaeIII GTNAC 2 cut(s) 265, 971
MalI GATC 9 cut(s) 114, 273, 330, 387, 414, 540, 549, 801, 1016
MboI GATC 9 cut(s) 112, 271, 328, 385, 412, 538, 547, 799, 1014
MboII GAAGA 6 cut(s) 54, 224, 452, 644, 726, 737
MflI RGATCY 3 cut(s) 271, 385, 538
MhlI GDGCHC 2 cut(s) 15, 830
MluCI AATT 7 cut(s) 289, 358, 524, 569, 601, 618, 900
MlyI GAGTC 1 cut(s) 853
MnlI CCTC 5 cut(s) 114, 137, 158, 563, 770
Mph1103I ATGCAT 1 cut(s) 400
MseI TTAA 2 cut(s) 707, 891
MslI CAYNNNNRTG 1 cut(s) 57
MspI CCGG 1 cut(s) 969
MspR9I CCNGG 1 cut(s) 838
MvaI CCWGG 1 cut(s) 838
MwoI GCNNNNNNNGC 2 cut(s) 10, 902
NcoI CCATGG 1 cut(s) 584
NdeI CATATG 3 cut(s) 85, 718, 820
NdeII GATC 9 cut(s) 112, 271, 328, 385, 412, 538, 547, 799, 1014
NlaIII CATG 4 cut(s) 335, 588, 806, 811
NlaIV GGNNCC 5 cut(s) 125, 387, 468, 540, 967
NmuCI GTSAC 2 cut(s) 265, 971
NsiI ATGCAT 1 cut(s) 400
PagI TCATGA 1 cut(s) 802
PciSI GCTCTTC 1 cut(s) 657
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 435
PfeI GAWTC 4 cut(s) 155, 235, 845, 877
PkrI GCNGC 1 cut(s) 1033
PleI GAGTC 1 cut(s) 853
PpsI GAGTC 1 cut(s) 853
Psp6I CCWGG 1 cut(s) 836
PspGI CCWGG 1 cut(s) 836
PspN4I GGNNCC 5 cut(s) 125, 387, 468, 540, 967
PsuI RGATCY 3 cut(s) 271, 385, 538
RsaI GTAC 3 cut(s) 300, 410, 663
RsaNI GTAC 3 cut(s) 299, 409, 662
RseI CAYNNNNRTG 1 cut(s) 57
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 2 cut(s) 707, 891
SatI GCNGC 1 cut(s) 1032
Sau3AI GATC 9 cut(s) 112, 271, 328, 385, 412, 538, 547, 799, 1014
ScaI AGTACT 1 cut(s) 300
SchI GAGTC 1 cut(s) 853
ScrFI CCNGG 1 cut(s) 838
SduI GDGCHC 2 cut(s) 15, 830
SfaNI GCATC 3 cut(s) 444, 523, 684
SfcI CTRYAG 2 cut(s) 775, 1043
SmiMI CAYNNNNRTG 1 cut(s) 57
SpeI ACTAGT 1 cut(s) 710
Sse9I AATT 7 cut(s) 289, 358, 524, 569, 601, 618, 900
SsiI CCGC 1 cut(s) 518
SspI AATATT 1 cut(s) 462
SspMI CTAG 1 cut(s) 711
StyD4I CCNGG 1 cut(s) 836
StyI CCWWGG 2 cut(s) 584, 881
TaaI ACNGT 5 cut(s) 36, 640, 742, 869, 958
TaiI ACGT 3 cut(s) 147, 424, 660
TaqI TCGA 2 cut(s) 56, 438
TasI AATT 7 cut(s) 289, 358, 524, 569, 601, 618, 900
TatI WGTACW 2 cut(s) 298, 661
TfiI GAWTC 4 cut(s) 155, 235, 845, 877
Tru1I TTAA 2 cut(s) 707, 891
Tru9I TTAA 2 cut(s) 707, 891
TseFI GTSAC 2 cut(s) 265, 971
TseI GCWGC 1 cut(s) 1031
Tsp45I GTSAC 2 cut(s) 265, 971
TspDTI ATGAA 3 cut(s) 225, 248, 705
XapI RAATTY 3 cut(s) 289, 524, 618
XmiI GTMKAC 1 cut(s) 923
XspI CTAG 1 cut(s) 711
ZrmI AGTACT 1 cut(s) 300
Zsp2I ATGCAT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.