Belongs to the peptidase S10 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 224 |
| AcsI | RAATTY | 3 cut(s) | 95, 315, 386 |
| AfeI | AGCGCT | 1 cut(s) | 267 |
| AgsI | TTSAA | 2 cut(s) | 353, 391 |
| AjnI | CCWGG | 1 cut(s) | 288 |
| AluBI | AGCT | 1 cut(s) | 80 |
| AluI | AGCT | 1 cut(s) | 80 |
| Alw26I | GTCTC | 1 cut(s) | 80 |
| Aor51HI | AGCGCT | 1 cut(s) | 267 |
| ApeKI | GCWGC | 1 cut(s) | 80 |
| ApoI | RAATTY | 3 cut(s) | 95, 315, 386 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 357 |
| AspLEI | GCGC | 1 cut(s) | 268 |
| BaeGI | GKGCMC | 1 cut(s) | 20 |
| BbvI | GCAGC | 1 cut(s) | 67 |
| BccI | CCATC | 1 cut(s) | 203 |
| BciT130I | CCWGG | 1 cut(s) | 290 |
| BciVI | GTATCC | 1 cut(s) | 44 |
| BcoDI | GTCTC | 1 cut(s) | 80 |
| BfmI | CTRYAG | 1 cut(s) | 81 |
| BfoI | RGCGCY | 1 cut(s) | 269 |
| BfuI | GTATCC | 1 cut(s) | 44 |
| BisI | GCNGC | 1 cut(s) | 81 |
| BlsI | GCNGC | 1 cut(s) | 82 |
| Bme1390I | CCNGG | 1 cut(s) | 290 |
| BmrFI | CCNGG | 1 cut(s) | 290 |
| BmsI | GCATC | 1 cut(s) | 373 |
| Bse1I | ACTGG | 1 cut(s) | 25 |
| BseBI | CCWGG | 1 cut(s) | 290 |
| BseMII | CTCAG | 1 cut(s) | 115 |
| BseNI | ACTGG | 1 cut(s) | 25 |
| BseSI | GKGCMC | 1 cut(s) | 20 |
| BseXI | GCAGC | 1 cut(s) | 67 |
| BsiSI | CCGG | 1 cut(s) | 156 |
| BsmAI | GTCTC | 1 cut(s) | 80 |
| Bsp1286I | GDGCHC | 1 cut(s) | 20 |
| BspCNI | CTCAG | 1 cut(s) | 114 |
| BspMAI | CTGCAG | 1 cut(s) | 85 |
| BsrI | ACTGG | 1 cut(s) | 25 |
| Bst2UI | CCWGG | 1 cut(s) | 290 |
| Bst4CI | ACNGT | 1 cut(s) | 380 |
| BstC8I | GCNNGC | 1 cut(s) | 6 |
| BstDEI | CTNAG | 2 cut(s) | 101, 220 |
| BstH2I | RGCGCY | 1 cut(s) | 269 |
| BstHHI | GCGC | 1 cut(s) | 268 |
| BstMAI | GTCTC | 1 cut(s) | 80 |
| BstNI | CCWGG | 1 cut(s) | 290 |
| BstNSI | RCATGY | 1 cut(s) | 8 |
| BstSCI | CCNGG | 1 cut(s) | 288 |
| BstSFI | CTRYAG | 1 cut(s) | 81 |
| BstSLI | GKGCMC | 1 cut(s) | 20 |
| BstV1I | GCAGC | 1 cut(s) | 67 |
| BsuI | GTATCC | 1 cut(s) | 44 |
| BtsIMutI | CAGTG | 1 cut(s) | 18 |
| Cac8I | GCNNGC | 1 cut(s) | 6 |
| CfoI | GCGC | 1 cut(s) | 268 |
| CsiI | ACCWGGT | 1 cut(s) | 288 |
| CviAII | CATG | 1 cut(s) | 5 |
| CviJI | RGCY | 4 cut(s) | 80, 302, 329, 347 |
| CviKI_1 | RGCY | 4 cut(s) | 80, 302, 329, 347 |
| DdeI | CTNAG | 2 cut(s) | 101, 220 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 224 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 224 |
| Eco47III | AGCGCT | 1 cut(s) | 267 |
| EcoRII | CCWGG | 1 cut(s) | 288 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| FaeI | CATG | 1 cut(s) | 8 |
| FatI | CATG | 1 cut(s) | 4 |
| FauNDI | CATATG | 1 cut(s) | 31 |
| Fnu4HI | GCNGC | 1 cut(s) | 81 |
| Fsp4HI | GCNGC | 1 cut(s) | 81 |
| GlaI | GCGC | 1 cut(s) | 267 |
| GluI | GCNGC | 1 cut(s) | 81 |
| HaeII | RGCGCY | 1 cut(s) | 269 |
| HapII | CCGG | 1 cut(s) | 156 |
| HhaI | GCGC | 1 cut(s) | 268 |
| Hin1II | CATG | 1 cut(s) | 8 |
| Hin6I | GCGC | 1 cut(s) | 266 |
| HinP1I | GCGC | 1 cut(s) | 266 |
| HinfI | GANTC | 3 cut(s) | 161, 322, 340 |
| HpaII | CCGG | 1 cut(s) | 156 |
| Hpy188I | TCNGA | 4 cut(s) | 104, 208, 260, 277 |
| Hpy188III | TCNNGA | 2 cut(s) | 197, 374 |
| HpyAV | CCTTC | 1 cut(s) | 41 |
| HpyCH4III | ACNGT | 1 cut(s) | 380 |
| HpyCH4V | TGCA | 7 cut(s) | 4, 8, 35, 83, 143, 295, 364 |
| HpyF3I | CTNAG | 2 cut(s) | 101, 220 |
| Hsp92II | CATG | 1 cut(s) | 8 |
| HspAI | GCGC | 1 cut(s) | 266 |
| LpnPI | CCDG | 9 cut(s) | 6, 24, 169, 182, 275, 284, 302, 311, 329 |
| Lsp1109I | GCAGC | 1 cut(s) | 67 |
| LweI | GCATC | 1 cut(s) | 373 |
| MabI | ACCWGGT | 1 cut(s) | 288 |
| MaeIII | GTNAC | 2 cut(s) | 23, 65 |
| MboII | GAAGA | 1 cut(s) | 173 |
| MhlI | GDGCHC | 1 cut(s) | 20 |
| MluCI | AATT | 4 cut(s) | 95, 235, 315, 386 |
| MnlI | CCTC | 1 cut(s) | 143 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MroXI | GAANNNNTTC | 1 cut(s) | 357 |
| MseI | TTAA | 1 cut(s) | 312 |
| MspA1I | CMGCKG | 1 cut(s) | 80 |
| MspI | CCGG | 1 cut(s) | 156 |
| MspR9I | CCNGG | 1 cut(s) | 290 |
| MvaI | CCWGG | 1 cut(s) | 290 |
| NdeI | CATATG | 1 cut(s) | 31 |
| NlaIII | CATG | 1 cut(s) | 8 |
| NmuCI | GTSAC | 1 cut(s) | 65 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 1 cut(s) | 8 |
| PaeI | GCATGC | 1 cut(s) | 8 |
| PdmI | GAANNNNTTC | 1 cut(s) | 357 |
| PfeI | GAWTC | 3 cut(s) | 161, 322, 340 |
| PkrI | GCNGC | 1 cut(s) | 82 |
| Psp6I | CCWGG | 1 cut(s) | 288 |
| PspGI | CCWGG | 1 cut(s) | 288 |
| PstI | CTGCAG | 1 cut(s) | 85 |
| PvuII | CAGCTG | 1 cut(s) | 80 |
| SaqAI | TTAA | 1 cut(s) | 312 |
| SatI | GCNGC | 1 cut(s) | 81 |
| ScrFI | CCNGG | 1 cut(s) | 290 |
| SduI | GDGCHC | 1 cut(s) | 20 |
| SetI | ASST | 3 cut(s) | 13, 82, 294 |
| SexAI | ACCWGGT | 1 cut(s) | 288 |
| SfaNI | GCATC | 1 cut(s) | 373 |
| SfcI | CTRYAG | 1 cut(s) | 81 |
| SphI | GCATGC | 1 cut(s) | 8 |
| Sse9I | AATT | 4 cut(s) | 95, 235, 315, 386 |
| StyD4I | CCNGG | 1 cut(s) | 288 |
| TaaI | ACNGT | 1 cut(s) | 380 |
| TaqI | TCGA | 1 cut(s) | 228 |
| TasI | AATT | 4 cut(s) | 95, 235, 315, 386 |
| TfiI | GAWTC | 3 cut(s) | 161, 322, 340 |
| Tru1I | TTAA | 1 cut(s) | 312 |
| Tru9I | TTAA | 1 cut(s) | 312 |
| TscAI | CASTG | 1 cut(s) | 25 |
| TseFI | GTSAC | 1 cut(s) | 65 |
| TseI | GCWGC | 1 cut(s) | 80 |
| Tsp45I | GTSAC | 1 cut(s) | 65 |
| TspDTI | ATGAA | 2 cut(s) | 108, 294 |
| TspRI | CASTG | 1 cut(s) | 25 |
| XapI | RAATTY | 3 cut(s) | 95, 315, 386 |
| XceI | RCATGY | 1 cut(s) | 8 |
| XmnI | GAANNNNTTC | 1 cut(s) | 357 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |