FvH4_6g49990

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000309
Physical Location & Seq
Reverse (-)
37305443 .. 37306787
1345 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g49990.t1

Sequence Viewer

Length: 942 bp
ATGGCACCAAAACCTGATCTTTCTCTTTTCACAGTCACTCTCCTCCTCCTTTTCCTTACCCTCTTTTCTTTCCTCTCTAATGCAACGGCAAAGGAAGTCCATAGCAGCAACAACTCATCTCCGTTTGACTTCCTTGACCATCTTAAGTGTTGTCACAAAGGTGATAAGGTCAAGGGCATCCATGACCTCAAGAAGTACCTTCACAAGTTCGGTTGCTTGGACTACATGAACCACATCCACTCTAATGACGACCATTTCGACCAGTTCCTGGAGGAAGCAGTCAGGACTTACCAGCTCAATTTCAACCTCAATCCCACGGGAACTTTGGACGAGAAAACTATATCAATGATGATGAAGCCTCATTGTGGTGTGCCCGATATCATCAATGGCACCACATCCATGCGATCGGCCAAGATAAAGCACCAACACCGTGCAATTCATACCACAGTTCACTACACATTGCCTGATGGAGATTATAAATGGCCACCCTCTAAATACCAACTCACTTTCGGTTTCCTCCCCGGCACCCCATCCGCAGCCACGGGTTCTATCGTGCAGGCTTTCGCAATATGGCAAAAGAGCACCCACTTCAAGTTCAGCCAGTCCAACAATCCCCAGAATGCTGATTTGAAGATCAGCTTTCACAAGCGTGATCATGGAGACGGGGATCCATTTGATGGGCCTGGTGGACAGCTCGCCCATGCAGCTTACCCTACGGGAGGAACACTGCACTACGACGCAGAGGAGACTTGGTCTGCAGGTGCTAAACCAGGTGCAATGGACTATGGGAGTGTTGGTTTGCATGAGATAGGGCACCTTCTGGGACTTGGACATAGTTCAGTAGAGGGAGCTGTCATGTTTCCAACTGTCACCGAGGGAGTGGCTTATAAAGGTTTGCATCAGGATGATATTCAAGGAATAAAAGCTTTGTACAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

314

Amino Acids

34.58

Weight (kDa)

6.61

Isoelectric Point (pI)

28.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 60 - 117 3.6e-11 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 165 - 311 3.5e-42 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 477, 888
AarI CACCTGC 1 cut(s) 749
Acc36I ACCTGC 1 cut(s) 749
AccB1I GGYRCC 4 cut(s) 4, 389, 524, 813
AccB7I CCANNNNNTGG 2 cut(s) 268, 677
AciI CCGC 1 cut(s) 534
AclWI GGATC 2 cut(s) 662, 675
AcoI YGGCCR 2 cut(s) 408, 482
AfaI GTAC 2 cut(s) 197, 932
AfiI CCNNNNNNNGG 4 cut(s) 268, 365, 677, 719
AflII CTTAAG 1 cut(s) 143
AgsI TTSAA 4 cut(s) 304, 592, 631, 914
AjnI CCWGG 3 cut(s) 267, 682, 769
AleI CACNNNNGTG 2 cut(s) 159, 648
AluBI AGCT 6 cut(s) 295, 639, 694, 707, 851, 926
AluI AGCT 6 cut(s) 295, 639, 694, 707, 851, 926
Alw21I GWGCWC 1 cut(s) 584
Alw26I GTCTC 2 cut(s) 654, 740
AlwI GGATC 2 cut(s) 662, 675
AlwNI CAGNNNCTG 1 cut(s) 268
AoxI GGCC 3 cut(s) 408, 482, 680
ApeKI GCWGC 3 cut(s) 105, 536, 704
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
AspS9I GGNCC 1 cut(s) 680
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 2 cut(s) 173, 862
BaeGI GKGCMC 2 cut(s) 375, 816
BalI TGGCCA 1 cut(s) 484
BamHI GGATCC 1 cut(s) 667
BanI GGYRCC 4 cut(s) 4, 389, 524, 813
Bbv12I GWGCWC 1 cut(s) 584
BbvI GCAGC 3 cut(s) 117, 548, 716
BccI CCATC 4 cut(s) 147, 461, 538, 671
BceAI ACGGC 1 cut(s) 102
BciT130I CCWGG 3 cut(s) 269, 684, 771
BclI TGATCA 1 cut(s) 652
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 2 cut(s) 654, 740
BfmI CTRYAG 1 cut(s) 756
BfrI CTTAAG 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 749
BisI GCNGC 3 cut(s) 106, 537, 705
BlsI GCNGC 3 cut(s) 107, 538, 706
Bme1390I CCNGG 4 cut(s) 269, 522, 684, 771
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 5 cut(s) 6, 391, 526, 669, 815
BmrFI CCNGG 4 cut(s) 269, 522, 684, 771
BmsI GCATC 2 cut(s) 186, 907
BpmI CTGGAG 1 cut(s) 290
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 522
BsaJI CCNNGG 4 cut(s) 315, 520, 540, 873
BsaXI ACNNNNNCTCC 4 cut(s) 737, 767, 781, 811
Bsc4I CCNNNNNNNGG 4 cut(s) 268, 365, 677, 719
Bse1I ACTGG 2 cut(s) 262, 601
Bse3DI GCAATG 2 cut(s) 458, 783
BseBI CCWGG 3 cut(s) 269, 684, 771
BseDI CCNNGG 4 cut(s) 315, 520, 540, 873
BseGI GGATG 5 cut(s) 177, 234, 395, 530, 910
BseLI CCNNNNNNNGG 4 cut(s) 268, 365, 677, 719
BseMI GCAATG 2 cut(s) 458, 783
BseNI ACTGG 2 cut(s) 262, 601
BseRI GAGGAG 3 cut(s) 32, 35, 758
BseSI GKGCMC 2 cut(s) 375, 816
BseXI GCAGC 3 cut(s) 117, 548, 716
BsgI GTGCAG 2 cut(s) 575, 713
Bsh1285I CGRYCG 1 cut(s) 407
BshFI GGCC 3 cut(s) 410, 484, 682
BshNI GGYRCC 4 cut(s) 4, 389, 524, 813
BsiEI CGRYCG 1 cut(s) 407
BsiHKAI GWGCWC 1 cut(s) 584
BsiSI CCGG 1 cut(s) 522
BslFI GGGAC 1 cut(s) 837
BslI CCNNNNNNNGG 4 cut(s) 268, 365, 677, 719
BsmAI GTCTC 2 cut(s) 654, 740
BsmBI CGTCTC 1 cut(s) 654
BsmFI GGGAC 1 cut(s) 837
BsmI GAATGC 1 cut(s) 625
BsnI GGCC 3 cut(s) 410, 484, 682
Bsp1286I GDGCHC 3 cut(s) 375, 584, 816
Bsp1407I TGTACA 1 cut(s) 930
Bsp143I GATC 5 cut(s) 16, 404, 633, 652, 667
BspACI CCGC 1 cut(s) 534
BspANI GGCC 3 cut(s) 410, 484, 682
BspLI GGNNCC 5 cut(s) 6, 391, 526, 669, 815
BspMAI CTGCAG 1 cut(s) 760
BspMI ACCTGC 1 cut(s) 749
BspPI GGATC 2 cut(s) 662, 675
BspT107I GGYRCC 4 cut(s) 4, 389, 524, 813
BspTI CTTAAG 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 458, 783
BsrGI TGTACA 1 cut(s) 930
BsrI ACTGG 2 cut(s) 262, 601
BssECI CCNNGG 4 cut(s) 315, 520, 540, 873
BssMI GATC 5 cut(s) 16, 404, 633, 652, 667
Bst2UI CCWGG 3 cut(s) 269, 684, 771
Bst4CI ACNGT 4 cut(s) 34, 431, 448, 868
BstAFI CTTAAG 1 cut(s) 143
BstAUI TGTACA 1 cut(s) 930
BstC8I GCNNGC 2 cut(s) 558, 696
BstDSI CCRYGG 2 cut(s) 315, 540
BstENI CCTNNNNNAGG 1 cut(s) 717
BstF5I GGATG 5 cut(s) 177, 234, 395, 530, 910
BstKTI GATC 5 cut(s) 19, 407, 636, 655, 670
BstMAI GTCTC 2 cut(s) 654, 740
BstMBI GATC 5 cut(s) 16, 404, 633, 652, 667
BstMCI CGRYCG 1 cut(s) 407
BstMWI GCNNNNNNNGC 1 cut(s) 704
BstNI CCWGG 3 cut(s) 269, 684, 771
BstSCI CCNGG 4 cut(s) 267, 520, 682, 769
BstSFI CTRYAG 1 cut(s) 756
BstSLI GKGCMC 2 cut(s) 375, 816
BstV1I GCAGC 3 cut(s) 117, 548, 716
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
BsuRI GGCC 3 cut(s) 410, 484, 682
BtgI CCRYGG 2 cut(s) 315, 540
BtsCI GGATG 5 cut(s) 177, 234, 395, 530, 910
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 1 cut(s) 725
BveI ACCTGC 1 cut(s) 749
Cac8I GCNNGC 2 cut(s) 558, 696
CaiI CAGNNNCTG 1 cut(s) 268
Cfr13I GGNCC 1 cut(s) 680
CseI GACGC 1 cut(s) 746
CsiI ACCWGGT 1 cut(s) 769
Csp6I GTAC 2 cut(s) 196, 931
CviAII CATG 7 cut(s) 182, 226, 400, 656, 701, 803, 856
CviQI GTAC 2 cut(s) 196, 931
DpnI GATC 5 cut(s) 18, 406, 635, 654, 669
DpnII GATC 5 cut(s) 16, 404, 633, 652, 667
EaeI YGGCCR 2 cut(s) 408, 482
Eco32I GATATC 1 cut(s) 379
EcoNI CCTNNNNNAGG 1 cut(s) 717
EcoRII CCWGG 3 cut(s) 267, 682, 769
EcoRV GATATC 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 654
FaeI CATG 7 cut(s) 185, 229, 403, 659, 704, 806, 859
FalI AAGNNNNNCTT 2 cut(s) 623, 655
FaqI GGGAC 1 cut(s) 837
FatI CATG 7 cut(s) 181, 225, 399, 655, 700, 802, 855
FbaI TGATCA 1 cut(s) 652
Fnu4HI GCNGC 3 cut(s) 106, 537, 705
FokI GGATG 5 cut(s) 164, 221, 382, 517, 917
Fsp4HI GCNGC 3 cut(s) 106, 537, 705
GluI GCNGC 3 cut(s) 106, 537, 705
GsuI CTGGAG 1 cut(s) 290
HaeIII GGCC 3 cut(s) 410, 484, 682
HapII CCGG 1 cut(s) 522
HgaI GACGC 1 cut(s) 746
Hin1II CATG 7 cut(s) 185, 229, 403, 659, 704, 806, 859
HindIII AAGCTT 1 cut(s) 924
HpaII CCGG 1 cut(s) 522
HphI GGTGA 2 cut(s) 173, 862
Hpy166II GTNNAC 2 cut(s) 451, 689
Hpy188III TCNNGA 3 cut(s) 190, 283, 902
Hpy8I GTNNAC 2 cut(s) 451, 689
Hpy99I CGWCG 1 cut(s) 740
HpyAV CCTTC 2 cut(s) 209, 827
HpyCH4III ACNGT 4 cut(s) 34, 431, 448, 868
HpyCH4V TGCA 9 cut(s) 83, 434, 556, 704, 730, 758, 776, 802, 898
HpyF10VI GCNNNNNNNGC 1 cut(s) 704
Hsp92II CATG 7 cut(s) 185, 229, 403, 659, 704, 806, 859
Ksp22I TGATCA 1 cut(s) 652
Kzo9I GATC 5 cut(s) 16, 404, 633, 652, 667
LmnI GCTCC 1 cut(s) 848
Lsp1109I GCAGC 3 cut(s) 117, 548, 716
LweI GCATC 2 cut(s) 186, 907
MabI ACCWGGT 1 cut(s) 769
MaeIII GTNAC 3 cut(s) 34, 152, 868
MalI GATC 5 cut(s) 18, 406, 635, 654, 669
MboI GATC 5 cut(s) 16, 404, 633, 652, 667
MboII GAAGA 1 cut(s) 643
MflI RGATCY 1 cut(s) 667
MhlI GDGCHC 3 cut(s) 375, 584, 816
MlsI TGGCCA 1 cut(s) 484
MluCI AATT 2 cut(s) 298, 435
MluNI TGGCCA 1 cut(s) 484
MmeI TCCRAC 2 cut(s) 630, 887
Mox20I TGGCCA 1 cut(s) 484
MscI TGGCCA 1 cut(s) 484
MseI TTAA 1 cut(s) 144
MslI CAYNNNNRTG 6 cut(s) 159, 243, 366, 398, 648, 903
Msp20I TGGCCA 1 cut(s) 484
MspCI CTTAAG 1 cut(s) 143
MspI CCGG 1 cut(s) 522
MspR9I CCNGG 4 cut(s) 269, 522, 684, 771
Mva1269I GAATGC 1 cut(s) 625
MvaI CCWGG 3 cut(s) 269, 684, 771
MwoI GCNNNNNNNGC 1 cut(s) 704
NciI CCSGG 1 cut(s) 522
NdeII GATC 5 cut(s) 16, 404, 633, 652, 667
NlaIII CATG 7 cut(s) 185, 229, 403, 659, 704, 806, 859
NlaIV GGNNCC 5 cut(s) 6, 391, 526, 669, 815
NmuCI GTSAC 3 cut(s) 34, 152, 868
OliI CACNNNNGTG 2 cut(s) 159, 648
PaqCI CACCTGC 1 cut(s) 749
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PctI GAATGC 1 cut(s) 625
PflFI GACNNNGTC 1 cut(s) 751
PflMI CCANNNNNTGG 2 cut(s) 268, 677
PfoI TCCNGGA 1 cut(s) 267
PkrI GCNGC 3 cut(s) 107, 538, 706
Ple19I CGATCG 1 cut(s) 407
PsiI TTATAA 2 cut(s) 477, 888
Psp6I CCWGG 3 cut(s) 267, 682, 769
PspGI CCWGG 3 cut(s) 267, 682, 769
PspN4I GGNNCC 5 cut(s) 6, 391, 526, 669, 815
PspPI GGNCC 1 cut(s) 680
PstI CTGCAG 1 cut(s) 760
PstNI CAGNNNCTG 1 cut(s) 268
PsuI RGATCY 1 cut(s) 667
PsyI GACNNNGTC 1 cut(s) 751
PvuI CGATCG 1 cut(s) 407
RsaI GTAC 2 cut(s) 197, 932
RsaNI GTAC 2 cut(s) 196, 931
RseI CAYNNNNRTG 6 cut(s) 159, 243, 366, 398, 648, 903
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 3 cut(s) 106, 537, 705
Sau3AI GATC 5 cut(s) 16, 404, 633, 652, 667
Sau96I GGNCC 1 cut(s) 680
ScrFI CCNGG 4 cut(s) 269, 522, 684, 771
SduI GDGCHC 3 cut(s) 375, 584, 816
SexAI ACCWGGT 1 cut(s) 769
SfaNI GCATC 2 cut(s) 186, 907
SfcI CTRYAG 1 cut(s) 756
SmiMI CAYNNNNRTG 6 cut(s) 159, 243, 366, 398, 648, 903
SmlI CTYRAG 2 cut(s) 143, 188
SmoI CTYRAG 2 cut(s) 143, 188
Sse9I AATT 2 cut(s) 298, 435
SsiI CCGC 1 cut(s) 534
StyD4I CCNGG 4 cut(s) 267, 520, 682, 769
TaaI ACNGT 4 cut(s) 34, 431, 448, 868
TaqI TCGA 1 cut(s) 258
TasI AATT 2 cut(s) 298, 435
TatI WGTACW 1 cut(s) 930
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TscAI CASTG 1 cut(s) 732
TseFI GTSAC 3 cut(s) 34, 152, 868
TseI GCWGC 3 cut(s) 105, 536, 704
Tsp45I GTSAC 3 cut(s) 34, 152, 868
TspDTI ATGAA 3 cut(s) 242, 368, 428
TspGWI ACGGA 1 cut(s) 111
TspRI CASTG 1 cut(s) 732
Tth111I GACNNNGTC 1 cut(s) 751
Van91I CCANNNNNTGG 2 cut(s) 268, 677
Vha464I CTTAAG 1 cut(s) 143
XagI CCTNNNNNAGG 1 cut(s) 717
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.