Rw0G015130

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00688
OG0000309
Physical Location & Seq
Reverse (-)
62 .. 2028
1967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015130.1

Sequence Viewer

Length: 837 bp
ATGGCACCAAAATCTTATCTTTCTCTTTTCACAGTCACTCTCCTTCTCCTCCTCCTCGCCCTCTTTTCTTTCCTCTGTAATGCAACGGCAAACTCATCTCCGTTTGAGGTCCTCGAGCATCTTAAGGGTTGTCACAAAGGTGACAAGGTCAAGGGCATCAACGACCTCAAGAAGTACCTTCAAAAGTTCGGTTACTTAAATTATGAGAACCACAGACATTCCAATGACGATGATTTTGACGAGCACTTGGAGGAAGCCGTCAAGACTTACCAGCTCAATTTCCACCTCAAGTCCACCGGAACATTGGACGACAAAACCCTATCAACGATGATGATGCCTCGTTGTGGTGTGCCTGATATCATCAATGGCACCACATCCATGCGATCAGCCCAGATAAAGCACCAGCACCGTGCAATTCATACACCACTGTTCACTTCTCATTTCCGATGGAAATCTAAAAATGGCCTTCTTCAAAATCCATCTGACTTACGGTTTTCTAGCCGCACCCCATCAGAAGCCCAGGGCGCTGTCGCACAGGCTTTCTCAACATGGGCTAAGAACACTCACTTCACATTCAGCAGGCTCAAAATTACCAGAGTGCCTTGTTTGAAGGTTAGCTTTCATAAGGGTGATCATGGAGACGGGAATTCATTTGATGGGCCTGGCGGGACAGCTGGCCCATGCTGCTACCCTACGGAGGGAGCATTGCACTACGACGCAGATGAGACTTGGTCTGTAGGTGCCAAACCAGGTGCCATGGACATTCAGAGTGTTGGTTTGCATGAAAATCGGGCACCTTCTGGGACTTGGACACAGCTCAGTGGAGGAAGCTATCAT
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

279

Amino Acids

30.96

Weight (kDa)

8.46

Isoelectric Point (pI)

23.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 53 - 110 1.5e-11 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 170 - 262 3.5e-18 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 4, 368, 740, 752, 793
AciI CCGC 2 cut(s) 502, 666
AcsI RAATTY 1 cut(s) 646
AfaI GTAC 1 cut(s) 176
AfiI CCNNNNNNNGG 3 cut(s) 344, 697, 698
AflII CTTAAG 1 cut(s) 122
AgsI TTSAA 3 cut(s) 182, 473, 610
AjnI CCWGG 3 cut(s) 519, 661, 748
AleI CACNNNNGTG 1 cut(s) 138
AluBI AGCT 5 cut(s) 274, 618, 674, 817, 831
AluI AGCT 5 cut(s) 274, 618, 674, 817, 831
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 2 cut(s) 633, 719
Ama87I CYCGRG 1 cut(s) 113
AoxI GGCC 3 cut(s) 463, 659, 676
ApeKI GCWGC 1 cut(s) 684
ApoI RAATTY 1 cut(s) 646
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
AspLEI GCGC 1 cut(s) 527
AspS9I GGNCC 3 cut(s) 109, 659, 677
AsuHPI GGTGA 2 cut(s) 152, 641
AvaI CYCGRG 1 cut(s) 113
AvaII GGWCC 1 cut(s) 109
BaeGI GKGCMC 1 cut(s) 796
BanI GGYRCC 5 cut(s) 4, 368, 740, 752, 793
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 671
BccI CCATC 4 cut(s) 441, 487, 517, 650
BceAI ACGGC 2 cut(s) 102, 242
BcgI CGANNNNNNTGC 4 cut(s) 316, 350, 770, 804
BciT130I CCWGG 3 cut(s) 521, 663, 750
BclI TGATCA 1 cut(s) 631
BcoDI GTCTC 2 cut(s) 633, 719
BfaI CTAG 1 cut(s) 498
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 1 cut(s) 528
BfrI CTTAAG 1 cut(s) 122
BisI GCNGC 2 cut(s) 502, 685
BlsI GCNGC 2 cut(s) 503, 686
Bme1390I CCNGG 3 cut(s) 521, 663, 750
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 113
BmgT120I GGNCC 3 cut(s) 109, 659, 677
BmiI GGNNCC 5 cut(s) 6, 370, 742, 754, 795
BmrFI CCNGG 3 cut(s) 521, 663, 750
BmsI GCATC 3 cut(s) 127, 165, 324
BpuEI CTTGAG 2 cut(s) 152, 272
BsaBI GATNNNNATC 1 cut(s) 451
BsaJI CCNNGG 3 cut(s) 519, 520, 756
BsaWI WCCGGW 1 cut(s) 296
Bsc4I CCNNNNNNNGG 3 cut(s) 344, 697, 698
Bse3DI GCAATG 1 cut(s) 704
Bse8I GATNNNNATC 1 cut(s) 451
BseBI CCWGG 3 cut(s) 521, 663, 750
BseDI CCNNGG 3 cut(s) 519, 520, 756
BseGI GGATG 1 cut(s) 374
BseJI GATNNNNATC 1 cut(s) 451
BseLI CCNNNNNNNGG 3 cut(s) 344, 697, 698
BseMI GCAATG 1 cut(s) 704
BseMII CTCAG 1 cut(s) 832
BseRI GAGGAG 3 cut(s) 38, 41, 44
BseSI GKGCMC 1 cut(s) 796
BseXI GCAGC 1 cut(s) 671
BshFI GGCC 3 cut(s) 465, 661, 678
BshNI GGYRCC 5 cut(s) 4, 368, 740, 752, 793
BsiHKAI GWGCWC 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 1 cut(s) 297
BslFI GGGAC 2 cut(s) 682, 817
BslI CCNNNNNNNGG 3 cut(s) 344, 697, 698
BsmAI GTCTC 2 cut(s) 633, 719
BsmBI CGTCTC 1 cut(s) 633
BsmFI GGGAC 2 cut(s) 682, 817
BsnI GGCC 3 cut(s) 465, 661, 678
BsoBI CYCGRG 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 246, 796
Bsp143I GATC 2 cut(s) 383, 631
Bsp19I CCATGG 1 cut(s) 756
BspACI CCGC 2 cut(s) 502, 666
BspANI GGCC 3 cut(s) 465, 661, 678
BspCNI CTCAG 1 cut(s) 831
BspLI GGNNCC 5 cut(s) 6, 370, 742, 754, 795
BspT107I GGYRCC 5 cut(s) 4, 368, 740, 752, 793
BspTI CTTAAG 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 704
BssECI CCNNGG 3 cut(s) 519, 520, 756
BssMI GATC 2 cut(s) 383, 631
BssT1I CCWWGG 1 cut(s) 756
Bst2UI CCWGG 3 cut(s) 521, 663, 750
Bst4CI ACNGT 4 cut(s) 34, 410, 429, 492
BstAFI CTTAAG 1 cut(s) 122
BstC8I GCNNGC 2 cut(s) 581, 676
BstDEI CTNAG 2 cut(s) 555, 818
BstDSI CCRYGG 1 cut(s) 756
BstF5I GGATG 1 cut(s) 374
BstH2I RGCGCY 1 cut(s) 528
BstHHI GCGC 1 cut(s) 527
BstKTI GATC 2 cut(s) 386, 634
BstMAI GTCTC 2 cut(s) 633, 719
BstMBI GATC 2 cut(s) 383, 631
BstMWI GCNNNNNNNGC 2 cut(s) 524, 684
BstNI CCWGG 3 cut(s) 521, 663, 750
BstSCI CCNGG 3 cut(s) 519, 661, 748
BstSFI CTRYAG 1 cut(s) 735
BstSLI GKGCMC 1 cut(s) 796
BstV1I GCAGC 1 cut(s) 671
BsuRI GGCC 3 cut(s) 465, 661, 678
BtgI CCRYGG 1 cut(s) 756
BtsCI GGATG 1 cut(s) 374
BtsIMutI CAGTG 2 cut(s) 425, 826
Cac8I GCNNGC 2 cut(s) 581, 676
CfoI GCGC 1 cut(s) 527
Cfr13I GGNCC 3 cut(s) 109, 659, 677
CseI GACGC 1 cut(s) 725
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 1 cut(s) 175
CviAII CATG 6 cut(s) 379, 549, 635, 681, 757, 782
CviQI GTAC 1 cut(s) 175
DdeI CTNAG 2 cut(s) 555, 818
DpnI GATC 2 cut(s) 385, 633
DpnII GATC 2 cut(s) 383, 631
Eco130I CCWWGG 1 cut(s) 756
Eco32I GATATC 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 113
EcoO109I RGGNCCY 1 cut(s) 109
EcoRI GAATTC 1 cut(s) 646
EcoRII CCWGG 3 cut(s) 519, 661, 748
EcoRV GATATC 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 756
Esp3I CGTCTC 1 cut(s) 633
FaeI CATG 6 cut(s) 382, 552, 638, 684, 760, 785
FaiI YATR 9 cut(s) 204, 380, 420, 550, 624, 636, 682, 758, 783
FalI AAGNNNNNCTT 2 cut(s) 602, 634
FaqI GGGAC 2 cut(s) 682, 817
FatI CATG 6 cut(s) 378, 548, 634, 680, 756, 781
FauI CCCGC 1 cut(s) 659
FbaI TGATCA 1 cut(s) 631
Fnu4HI GCNGC 2 cut(s) 502, 685
FokI GGATG 1 cut(s) 361
Fsp4HI GCNGC 2 cut(s) 502, 685
FspBI CTAG 1 cut(s) 498
GlaI GCGC 1 cut(s) 526
GluI GCNGC 2 cut(s) 502, 685
HaeII RGCGCY 1 cut(s) 528
HaeIII GGCC 3 cut(s) 465, 661, 678
HapII CCGG 1 cut(s) 297
HgaI GACGC 1 cut(s) 725
HhaI GCGC 1 cut(s) 527
Hin1II CATG 6 cut(s) 382, 552, 638, 684, 760, 785
Hin6I GCGC 1 cut(s) 525
HinP1I GCGC 1 cut(s) 525
HpaII CCGG 1 cut(s) 297
HphI GGTGA 2 cut(s) 152, 641
Hpy166II GTNNAC 2 cut(s) 294, 432
Hpy188I TCNGA 4 cut(s) 446, 484, 514, 768
Hpy188III TCNNGA 2 cut(s) 169, 262
Hpy8I GTNNAC 2 cut(s) 294, 432
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 5 cut(s) 53, 188, 476, 604, 807
HpyCH4III ACNGT 4 cut(s) 34, 410, 429, 492
HpyCH4V TGCA 4 cut(s) 83, 413, 709, 781
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 684
HpyF3I CTNAG 2 cut(s) 555, 818
Hsp92II CATG 6 cut(s) 382, 552, 638, 684, 760, 785
HspAI GCGC 1 cut(s) 525
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 2 cut(s) 383, 631
LmnI GCTCC 1 cut(s) 701
Lsp1109I GCAGC 1 cut(s) 671
LweI GCATC 3 cut(s) 127, 165, 324
MabI ACCWGGT 1 cut(s) 748
MaeI CTAG 1 cut(s) 498
MaeIII GTNAC 4 cut(s) 34, 131, 140, 191
MalI GATC 2 cut(s) 385, 633
MboI GATC 2 cut(s) 383, 631
MboII GAAGA 1 cut(s) 461
MhlI GDGCHC 2 cut(s) 246, 796
MluCI AATT 5 cut(s) 199, 277, 414, 588, 646
MseI TTAA 2 cut(s) 123, 197
MslI CAYNNNNRTG 4 cut(s) 138, 222, 377, 627
MspA1I CMGCKG 1 cut(s) 674
MspCI CTTAAG 1 cut(s) 122
MspI CCGG 1 cut(s) 297
MspR9I CCNGG 3 cut(s) 521, 663, 750
MvaI CCWGG 3 cut(s) 521, 663, 750
MwoI GCNNNNNNNGC 2 cut(s) 524, 684
NcoI CCATGG 1 cut(s) 756
NdeII GATC 2 cut(s) 383, 631
NlaIII CATG 6 cut(s) 382, 552, 638, 684, 760, 785
NlaIV GGNNCC 5 cut(s) 6, 370, 742, 754, 795
NmuCI GTSAC 3 cut(s) 34, 131, 140
OliI CACNNNNGTG 1 cut(s) 138
PaeR7I CTCGAG 1 cut(s) 113
PasI CCCWGGG 1 cut(s) 520
PflFI GACNNNGTC 2 cut(s) 146, 730
PkrI GCNGC 2 cut(s) 503, 686
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 3 cut(s) 519, 661, 748
PspGI CCWGG 3 cut(s) 519, 661, 748
PspN4I GGNNCC 5 cut(s) 6, 370, 742, 754, 795
PspPI GGNCC 3 cut(s) 109, 659, 677
PspPPI RGGWCCY 1 cut(s) 109
PspXI VCTCGAGB 1 cut(s) 113
PsyI GACNNNGTC 2 cut(s) 146, 730
PvuII CAGCTG 1 cut(s) 674
RsaI GTAC 1 cut(s) 176
RsaNI GTAC 1 cut(s) 175
RseI CAYNNNNRTG 4 cut(s) 138, 222, 377, 627
SaqAI TTAA 2 cut(s) 123, 197
SatI GCNGC 2 cut(s) 502, 685
Sau3AI GATC 2 cut(s) 383, 631
Sau96I GGNCC 3 cut(s) 109, 659, 677
ScrFI CCNGG 3 cut(s) 521, 663, 750
SduI GDGCHC 2 cut(s) 246, 796
SexAI ACCWGGT 1 cut(s) 748
SfaNI GCATC 3 cut(s) 127, 165, 324
SfcI CTRYAG 1 cut(s) 735
Sfr274I CTCGAG 1 cut(s) 113
SinI GGWCC 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 113
SmiMI CAYNNNNRTG 4 cut(s) 138, 222, 377, 627
SmlI CTYRAG 4 cut(s) 113, 122, 167, 287
SmoI CTYRAG 4 cut(s) 113, 122, 167, 287
Sse9I AATT 5 cut(s) 199, 277, 414, 588, 646
SsiI CCGC 2 cut(s) 502, 666
SspMI CTAG 1 cut(s) 498
StyD4I CCNGG 3 cut(s) 519, 661, 748
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 4 cut(s) 34, 410, 429, 492
TaqI TCGA 1 cut(s) 114
TasI AATT 5 cut(s) 199, 277, 414, 588, 646
TauI GCSGC 1 cut(s) 504
Tru1I TTAA 2 cut(s) 123, 197
Tru9I TTAA 2 cut(s) 123, 197
TscAI CASTG 2 cut(s) 432, 826
TseFI GTSAC 3 cut(s) 34, 131, 140
TseI GCWGC 1 cut(s) 684
Tsp45I GTSAC 3 cut(s) 34, 131, 140
TspDTI ATGAA 4 cut(s) 407, 611, 639, 798
TspGWI ACGGA 2 cut(s) 90, 710
TspRI CASTG 2 cut(s) 432, 826
Tth111I GACNNNGTC 2 cut(s) 146, 730
Vha464I CTTAAG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 109
XapI RAATTY 1 cut(s) 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XhoI CTCGAG 1 cut(s) 113
XspI CTAG 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.