Prupe.3G277800_v2.0.a1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000309
Physical Location & Seq
Reverse (-)
25511958 .. 25512740
783 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G277800.1

Sequence Viewer

Length: 783 bp
GGGTGTCACAAGGGTGACAAAGTTCAAGGCATCCAAGACCTCAAAAAATACCTTGAAAAGTTTGGTTACTTGAATGTCCATACCAACAATGATGACTACTTTGATGACGAACTGGAGTCGGCCATCAAAACTTACCAAATCAATTACCACCTCAAGGTCACCGGAACATTAGATGCGAAAACCGTAAAAAAAATGGAGATGCCTCGATGTGGTGTACCGGATATCATCAACGGTACCACCTCCATGAGATCAGGAAAGAAAAGGGGAGGACATGGTTCAATTCACACAGTTGGTCATTACGCTTTCTTCCAAGGAAACCCAAAATGGCCTGCTAATAAGTATCACCTCACCTATGGTTTTCTTCAAGGCACCCCATCTGAGGCTGTGGGCGCAGTTGCACGTGCTTTTGCAACATGGCAGGGCAACACACACTTCACGTTCAGCCAAGCCCAAAGCATTGAGAGCGCTGATCTGAAGATCGGTTTTGGAAGGCGTGATCATGGAGATGGACACCCGTTTGATGGGCCGTATGGGACCGCTGCCCATGCATTTGCGCCAACAGATGGGAGATTTCACTACGATGCTGATGAGACGTGGGTTGTGGGTGCTGTGCCTGGTGGTTTGGACTTGGAGACTGTGGCTTTGCATGAAATTGGGCACCTTCTTGGGCTTGGCCATAGCTCTGTTCCAGGAGCTGTCATGCTTCCTGAAGTCCGCACTGGATTTACCCAGAGCTTGCATGCGGATGATATTCAAGGAATTAAAGCTTTATACAACACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

261

Amino Acids

28.37

Weight (kDa)

6.47

Isoelectric Point (pI)

29.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 3 cut(s) 233, 368, 657
AccB7I CCANNNNNTGG 1 cut(s) 563
AciI CCGC 3 cut(s) 537, 715, 743
AcoI YGGCCR 2 cut(s) 120, 673
AcuI CTGAAG 2 cut(s) 494, 729
AcvI CACGTG 1 cut(s) 401
AfaI GTAC 2 cut(s) 216, 235
AfeI AGCGCT 1 cut(s) 466
AfiI CCNNNNNNNGG 5 cut(s) 154, 209, 379, 521, 563
AgsI TTSAA 6 cut(s) 26, 56, 73, 279, 365, 755
AjiI CACGTC 1 cut(s) 594
AjnI CCWGG 2 cut(s) 613, 688
AleI CACNNNNGTG 1 cut(s) 12
AluBI AGCT 4 cut(s) 681, 695, 735, 767
AluI AGCT 4 cut(s) 681, 695, 735, 767
Alw26I GTCTC 2 cut(s) 584, 626
AlwNI CAGNNNCTG 1 cut(s) 695
Aor51HI AGCGCT 1 cut(s) 466
AoxI GGCC 4 cut(s) 120, 326, 524, 673
ApeKI GCWGC 1 cut(s) 539
ArsI GACNNNNNNTTYG 2 cut(s) 625, 657
Asp718I GGTACC 1 cut(s) 233
AspLEI GCGC 3 cut(s) 392, 467, 556
AspS9I GGNCC 2 cut(s) 524, 534
AsuHPI GGTGA 4 cut(s) 26, 151, 335, 340
AvaII GGWCC 1 cut(s) 534
BaeGI GKGCMC 1 cut(s) 660
BalI TGGCCA 1 cut(s) 675
BanI GGYRCC 3 cut(s) 233, 368, 657
BbrPI CACGTG 1 cut(s) 401
BbvI GCAGC 1 cut(s) 526
BccI CCATC 5 cut(s) 131, 382, 500, 515, 557
BceAI ACGGC 1 cut(s) 511
BciT130I CCWGG 2 cut(s) 615, 690
BclI TGATCA 1 cut(s) 496
BcoDI GTCTC 2 cut(s) 584, 626
BfoI RGCGCY 1 cut(s) 468
BisI GCNGC 1 cut(s) 540
BlsI GCNGC 1 cut(s) 541
Bme1390I CCNGG 2 cut(s) 615, 690
Bme18I GGWCC 1 cut(s) 534
BmgBI CACGTC 1 cut(s) 594
BmgT120I GGNCC 2 cut(s) 524, 534
BmiI GGNNCC 4 cut(s) 235, 370, 535, 659
BmrFI CCNGG 2 cut(s) 615, 690
BmsI GCATC 4 cut(s) 39, 163, 189, 571
BpmI CTGGAG 1 cut(s) 134
BpuEI CTTGAG 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 401
BsaJI CCNNGG 1 cut(s) 310
BsaWI WCCGGW 2 cut(s) 161, 217
Bsc4I CCNNNNNNNGG 5 cut(s) 154, 209, 379, 521, 563
Bse1I ACTGG 2 cut(s) 117, 724
BseBI CCWGG 2 cut(s) 615, 690
BseDI CCNNGG 1 cut(s) 310
BseGI GGATG 2 cut(s) 30, 751
BseLI CCNNNNNNNGG 5 cut(s) 154, 209, 379, 521, 563
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 2 cut(s) 117, 724
BseSI GKGCMC 1 cut(s) 660
BseXI GCAGC 1 cut(s) 526
BshFI GGCC 4 cut(s) 122, 328, 526, 675
BshNI GGYRCC 3 cut(s) 233, 368, 657
BsiSI CCGG 2 cut(s) 162, 218
BslFI GGGAC 1 cut(s) 547
BslI CCNNNNNNNGG 5 cut(s) 154, 209, 379, 521, 563
BsmAI GTCTC 2 cut(s) 584, 626
BsmBI CGTCTC 1 cut(s) 584
BsmFI GGGAC 1 cut(s) 547
BsnI GGCC 4 cut(s) 122, 328, 526, 675
Bsp1286I GDGCHC 1 cut(s) 660
Bsp143I GATC 4 cut(s) 248, 469, 477, 496
BspACI CCGC 3 cut(s) 537, 715, 743
BspANI GGCC 4 cut(s) 122, 328, 526, 675
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 4 cut(s) 235, 370, 535, 659
BspT107I GGYRCC 3 cut(s) 233, 368, 657
BsrI ACTGG 2 cut(s) 117, 724
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 4 cut(s) 248, 469, 477, 496
BssT1I CCWWGG 1 cut(s) 310
Bst2UI CCWGG 2 cut(s) 615, 690
Bst4CI ACNGT 4 cut(s) 184, 233, 289, 637
BstBAI YACGTR 1 cut(s) 401
BstC8I GCNNGC 3 cut(s) 330, 737, 741
BstDEI CTNAG 1 cut(s) 378
BstEII GGTNACC 1 cut(s) 157
BstF5I GGATG 2 cut(s) 30, 751
BstH2I RGCGCY 1 cut(s) 468
BstHHI GCGC 3 cut(s) 392, 467, 556
BstKTI GATC 4 cut(s) 251, 472, 480, 499
BstMAI GTCTC 2 cut(s) 584, 626
BstMBI GATC 4 cut(s) 248, 469, 477, 496
BstMWI GCNNNNNNNGC 3 cut(s) 389, 462, 545
BstNI CCWGG 2 cut(s) 615, 690
BstNSI RCATGY 1 cut(s) 743
BstPI GGTNACC 1 cut(s) 157
BstSCI CCNGG 2 cut(s) 613, 688
BstSLI GKGCMC 1 cut(s) 660
BstV1I GCAGC 1 cut(s) 526
BsuRI GGCC 4 cut(s) 122, 328, 526, 675
BtrI CACGTC 1 cut(s) 594
BtsCI GGATG 2 cut(s) 30, 751
BtsIMutI CAGTG 1 cut(s) 717
Cac8I GCNNGC 3 cut(s) 330, 737, 741
CaiI CAGNNNCTG 1 cut(s) 695
CfoI GCGC 3 cut(s) 392, 467, 556
Cfr13I GGNCC 2 cut(s) 524, 534
Csp6I GTAC 2 cut(s) 215, 234
CviAII CATG 8 cut(s) 244, 272, 414, 500, 545, 647, 700, 740
CviQI GTAC 2 cut(s) 215, 234
DdeI CTNAG 1 cut(s) 378
DpnI GATC 4 cut(s) 250, 471, 479, 498
DpnII GATC 4 cut(s) 248, 469, 477, 496
EaeI YGGCCR 2 cut(s) 120, 673
Eco130I CCWWGG 1 cut(s) 310
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 534
Eco47III AGCGCT 1 cut(s) 466
Eco57I CTGAAG 2 cut(s) 494, 729
Eco72I CACGTG 1 cut(s) 401
Eco91I GGTNACC 1 cut(s) 157
EcoO65I GGTNACC 1 cut(s) 157
EcoRII CCWGG 2 cut(s) 613, 688
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 550
ErhI CCWWGG 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 584
FaeI CATG 8 cut(s) 247, 275, 417, 503, 548, 650, 703, 743
FaqI GGGAC 1 cut(s) 547
FatI CATG 8 cut(s) 243, 271, 413, 499, 544, 646, 699, 739
FbaI TGATCA 1 cut(s) 496
Fnu4HI GCNGC 1 cut(s) 540
FokI GGATG 2 cut(s) 17, 758
Fsp4HI GCNGC 1 cut(s) 540
GlaI GCGC 3 cut(s) 391, 466, 555
GluI GCNGC 1 cut(s) 540
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 468
HaeIII GGCC 4 cut(s) 122, 328, 526, 675
HapII CCGG 2 cut(s) 162, 218
HhaI GCGC 3 cut(s) 392, 467, 556
Hin1II CATG 8 cut(s) 247, 275, 417, 503, 548, 650, 703, 743
Hin6I GCGC 3 cut(s) 390, 465, 554
HinP1I GCGC 3 cut(s) 390, 465, 554
HindIII AAGCTT 1 cut(s) 765
HinfI GANTC 1 cut(s) 116
HpaII CCGG 2 cut(s) 162, 218
HphI GGTGA 4 cut(s) 26, 151, 335, 340
Hpy166II GTNNAC 1 cut(s) 215
Hpy188I TCNGA 2 cut(s) 379, 474
Hpy188III TCNNGA 2 cut(s) 252, 707
Hpy8I GTNNAC 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 483, 671
HpyCH4III ACNGT 4 cut(s) 184, 233, 289, 637
HpyCH4IV ACGT 3 cut(s) 400, 437, 593
HpyCH4V TGCA 5 cut(s) 398, 410, 548, 646, 739
HpyF10VI GCNNNNNNNGC 3 cut(s) 389, 462, 545
HpyF3I CTNAG 1 cut(s) 378
HpySE526I ACGT 3 cut(s) 400, 437, 593
Hsp92II CATG 8 cut(s) 247, 275, 417, 503, 548, 650, 703, 743
HspAI GCGC 3 cut(s) 390, 465, 554
KpnI GGTACC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 496
Kzo9I GATC 4 cut(s) 248, 469, 477, 496
LmnI GCTCC 1 cut(s) 692
Lsp1109I GCAGC 1 cut(s) 526
LweI GCATC 4 cut(s) 39, 163, 189, 571
MaeII ACGT 3 cut(s) 400, 437, 593
MaeIII GTNAC 4 cut(s) 5, 14, 65, 157
MalI GATC 4 cut(s) 250, 471, 479, 498
MboI GATC 4 cut(s) 248, 469, 477, 496
MboII GAAGA 3 cut(s) 298, 353, 487
MhlI GDGCHC 1 cut(s) 660
MlsI TGGCCA 1 cut(s) 675
MluCI AATT 4 cut(s) 142, 279, 651, 759
MluNI TGGCCA 1 cut(s) 675
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 7 cut(s) 50, 161, 213, 250, 260, 356, 373
Mox20I TGGCCA 1 cut(s) 675
Mph1103I ATGCAT 1 cut(s) 550
MscI TGGCCA 1 cut(s) 675
MseI TTAA 1 cut(s) 762
MslI CAYNNNNRTG 5 cut(s) 12, 242, 504, 579, 744
Msp20I TGGCCA 1 cut(s) 675
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 2 cut(s) 162, 218
MspR9I CCNGG 2 cut(s) 615, 690
MvaI CCWGG 2 cut(s) 615, 690
MwoI GCNNNNNNNGC 3 cut(s) 389, 462, 545
NdeII GATC 4 cut(s) 248, 469, 477, 496
NlaIII CATG 8 cut(s) 247, 275, 417, 503, 548, 650, 703, 743
NlaIV GGNNCC 4 cut(s) 235, 370, 535, 659
NmuCI GTSAC 3 cut(s) 5, 14, 157
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 1 cut(s) 743
OliI CACNNNNGTG 1 cut(s) 12
PaeI GCATGC 1 cut(s) 743
PflMI CCANNNNNTGG 1 cut(s) 563
PfoI TCCNGGA 1 cut(s) 688
PkrI GCNGC 1 cut(s) 541
PleI GAGTC 1 cut(s) 124
PmaCI CACGTG 1 cut(s) 401
PmlI CACGTG 1 cut(s) 401
PpsI GAGTC 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 401
Psp6I CCWGG 2 cut(s) 613, 688
PspCI CACGTG 1 cut(s) 401
PspEI GGTNACC 1 cut(s) 157
PspGI CCWGG 2 cut(s) 613, 688
PspN4I GGNNCC 4 cut(s) 235, 370, 535, 659
PspPI GGNCC 2 cut(s) 524, 534
PstNI CAGNNNCTG 1 cut(s) 695
RsaI GTAC 2 cut(s) 216, 235
RsaNI GTAC 2 cut(s) 215, 234
RseI CAYNNNNRTG 5 cut(s) 12, 242, 504, 579, 744
SaqAI TTAA 1 cut(s) 762
SatI GCNGC 1 cut(s) 540
Sau3AI GATC 4 cut(s) 248, 469, 477, 496
Sau96I GGNCC 2 cut(s) 524, 534
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 615, 690
SduI GDGCHC 1 cut(s) 660
SfaNI GCATC 4 cut(s) 39, 163, 189, 571
SinI GGWCC 1 cut(s) 534
SmiMI CAYNNNNRTG 5 cut(s) 12, 242, 504, 579, 744
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
SphI GCATGC 1 cut(s) 743
Sse9I AATT 4 cut(s) 142, 279, 651, 759
SsiI CCGC 3 cut(s) 537, 715, 743
StyD4I CCNGG 2 cut(s) 613, 688
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 4 cut(s) 184, 233, 289, 637
TaiI ACGT 3 cut(s) 403, 440, 596
TaqI TCGA 1 cut(s) 205
TasI AATT 4 cut(s) 142, 279, 651, 759
Tru1I TTAA 1 cut(s) 762
Tru9I TTAA 1 cut(s) 762
TscAI CASTG 1 cut(s) 724
TseFI GTSAC 3 cut(s) 5, 14, 157
TseI GCWGC 1 cut(s) 539
Tsp45I GTSAC 3 cut(s) 5, 14, 157
TspDTI ATGAA 1 cut(s) 663
TspRI CASTG 1 cut(s) 724
Van91I CCANNNNNTGG 1 cut(s) 563
VpaK11BI GGWCC 1 cut(s) 534
XceI RCATGY 1 cut(s) 743
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.