Rh2AG618800

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000309
Physical Location & Seq
Reverse (-)
84259362 .. 84260579
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG618800.1

Sequence Viewer

Length: 819 bp
ATGGCATCCAAATCTAATCTTTCTCTCTTCACTGTCACTCTCCTCCTTGCCCTTCTTACTCTCCTCTCTTATGCAACTGCTTCTCATCCCCACAACAAGAGCTCTTCCCCATTTGAGTTCCTCGAGCATCTCAAGGGTTGCCACAAAGGTGATAAGGTCAAGGGCATCAATGATCTCAAAAAATACCTAGAAAAGTACGGTTACTTGAACTACAAGAACCACAGTCATTCGGACGACGATGATTTTGATGACCTTTTGGAGGAAGCCATCAAGACTTACCAGGTCAATTACCACCTCAAGTCCACCGGAACACTGGACGGCAAAACAGTGTCGAAGATGATGACACCTCGTTGTGGCGTGCCGGATATCATCAATGGCACCTCGTCCATGAGATCAGCCAAGAAAAGGCACCACCATGCCACGGGTGCTGTCGCACGGGCTTTCGCAACATGGGCTGGAAACACTCACTTCACGTTCAGCCAGTCCCAAAGTTACGAGAATGCGGATTTGAAGATTAGTTTTCATAAGGGTGACCATGGAGACGGGAATTCATTTGACGGCCCGGGCGGGATCCTAGCCCATGCTTTTAGCCCGTCGGATGGAAGATTCCACTACGACGCAGATGAGCCTTGGTCTGTGGGTGCTACACCAGGTGCTTATGACTTGGAGACTGTGGCTTTGCATGAAATAGGGCACCTTCTGGGACTTGGACATAGCTCAGTGGAGGGAGCTATCATGGCCCCAGCTATTCCCCCGGGAGTGACTAAAAGTTTGCATGGGGACGATGTTCAAGGAATCAAGGCTTTATACAACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

272

Amino Acids

29.52

Weight (kDa)

6.42

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 56 - 113 2.2e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 135 - 270 1.7e-44 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 377, 408, 693
AciI CCGC 2 cut(s) 503, 567
AclWI GGATC 2 cut(s) 565, 578
AcsI RAATTY 1 cut(s) 547
AdeI CACNNNGTG 1 cut(s) 653
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 5 cut(s) 259, 353, 405, 421, 599
AgsI TTSAA 3 cut(s) 208, 511, 791
AjnI CCWGG 2 cut(s) 279, 649
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 4 cut(s) 102, 717, 731, 746
AluI AGCT 4 cut(s) 102, 717, 731, 746
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 2 cut(s) 534, 662
AlwI GGATC 2 cut(s) 565, 578
Ama87I CYCGRG 3 cut(s) 122, 562, 754
AoxI GGCC 2 cut(s) 559, 738
ApoI RAATTY 1 cut(s) 547
ArsI GACNNNNNNTTYG 6 cut(s) 227, 259, 661, 693, 754, 786
AspS9I GGNCC 2 cut(s) 560, 739
AsuC2I CCSGG 4 cut(s) 563, 564, 755, 756
AsuHPI GGTGA 2 cut(s) 161, 542
AvaI CYCGRG 3 cut(s) 122, 562, 754
BaeGI GKGCMC 1 cut(s) 696
BamHI GGATCC 1 cut(s) 570
BanI GGYRCC 3 cut(s) 377, 408, 693
BanII GRGCYC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 104
BccI CCATC 2 cut(s) 275, 593
BceAI ACGGC 2 cut(s) 334, 574
BciT130I CCWGG 2 cut(s) 281, 651
BcnI CCSGG 4 cut(s) 563, 564, 755, 756
BcoDI GTCTC 2 cut(s) 534, 662
BfaI CTAG 2 cut(s) 188, 575
Bme1390I CCNGG 6 cut(s) 281, 563, 564, 651, 755, 756
BmeT110I CYCGRG 3 cut(s) 122, 562, 754
BmgT120I GGNCC 2 cut(s) 560, 739
BmiI GGNNCC 5 cut(s) 379, 410, 572, 695, 741
BmrFI CCNGG 6 cut(s) 281, 563, 564, 651, 755, 756
BmsI GCATC 3 cut(s) 14, 136, 174
BpuEI CTTGAG 2 cut(s) 116, 281
BpuMI CCSGG 4 cut(s) 563, 564, 755, 756
BsaJI CCNNGG 6 cut(s) 420, 535, 562, 629, 753, 754
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 353, 405, 421, 599
Bse1I ACTGG 2 cut(s) 318, 481
BseBI CCWGG 2 cut(s) 281, 651
BseDI CCNNGG 6 cut(s) 420, 535, 562, 629, 753, 754
BseGI GGATG 3 cut(s) 5, 85, 604
BseLI CCNNNNNNNGG 5 cut(s) 259, 353, 405, 421, 599
BseMII CTCAG 1 cut(s) 732
BseNI ACTGG 2 cut(s) 318, 481
BseRI GAGGAG 2 cut(s) 32, 53
BseSI GKGCMC 1 cut(s) 696
BseYI CCCAGC 1 cut(s) 742
BshFI GGCC 2 cut(s) 561, 740
BshNI GGYRCC 3 cut(s) 377, 408, 693
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 3 cut(s) 122, 562, 754
BsiSI CCGG 4 cut(s) 306, 362, 563, 755
BslFI GGGAC 3 cut(s) 469, 717, 794
BslI CCNNNNNNNGG 5 cut(s) 259, 353, 405, 421, 599
BsmAI GTCTC 2 cut(s) 534, 662
BsmBI CGTCTC 1 cut(s) 534
BsmFI GGGAC 3 cut(s) 469, 717, 794
BsmI GAATGC 1 cut(s) 505
BsnI GGCC 2 cut(s) 561, 740
BsoBI CYCGRG 3 cut(s) 122, 562, 754
Bsp1286I GDGCHC 2 cut(s) 104, 696
Bsp143I GATC 3 cut(s) 172, 392, 570
Bsp19I CCATGG 1 cut(s) 535
BspACI CCGC 2 cut(s) 503, 567
BspANI GGCC 2 cut(s) 561, 740
BspCNI CTCAG 1 cut(s) 731
BspLI GGNNCC 5 cut(s) 379, 410, 572, 695, 741
BspPI GGATC 2 cut(s) 565, 578
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 3 cut(s) 377, 408, 693
BsrI ACTGG 2 cut(s) 318, 481
BssECI CCNNGG 6 cut(s) 420, 535, 562, 629, 753, 754
BssMI GATC 3 cut(s) 172, 392, 570
BssT1I CCWWGG 2 cut(s) 535, 629
Bst2UI CCWGG 2 cut(s) 281, 651
Bst4CI ACNGT 5 cut(s) 34, 200, 224, 328, 673
Bst6I CTCTTC 2 cut(s) 32, 109
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 718
BstDSI CCRYGG 2 cut(s) 420, 535
BstEII GGTNACC 1 cut(s) 530
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 3 cut(s) 5, 85, 604
BstKTI GATC 3 cut(s) 175, 395, 573
BstMAI GTCTC 2 cut(s) 534, 662
BstMBI GATC 3 cut(s) 172, 392, 570
BstMWI GCNNNNNNNGC 3 cut(s) 425, 452, 737
BstNI CCWGG 2 cut(s) 281, 651
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 6 cut(s) 279, 561, 562, 649, 753, 754
BstSLI GKGCMC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 570
BstYI RGATCY 1 cut(s) 570
BsuRI GGCC 2 cut(s) 561, 740
BtgI CCRYGG 2 cut(s) 420, 535
BtsCI GGATG 3 cut(s) 5, 85, 604
BtsIMutI CAGTG 4 cut(s) 30, 311, 333, 726
Cac8I GCNNGC 1 cut(s) 359
Cfr13I GGNCC 2 cut(s) 560, 739
Cfr9I CCCGGG 2 cut(s) 562, 754
CseI GACGC 1 cut(s) 626
CsiI ACCWGGT 2 cut(s) 279, 649
Csp6I GTAC 1 cut(s) 196
CviAII CATG 8 cut(s) 388, 416, 450, 536, 581, 683, 736, 776
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 718
DpnI GATC 3 cut(s) 174, 394, 572
DpnII GATC 3 cut(s) 172, 392, 570
DraIII CACNNNGTG 1 cut(s) 653
Eam1104I CTCTTC 2 cut(s) 32, 109
EarI CTCTTC 2 cut(s) 32, 109
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 535, 629
Eco24I GRGCYC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 367
Eco53kI GAGCTC 1 cut(s) 102
Eco88I CYCGRG 3 cut(s) 122, 562, 754
Eco91I GGTNACC 1 cut(s) 530
EcoICRI GAGCTC 1 cut(s) 102
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 547
EcoRII CCWGG 2 cut(s) 279, 649
EcoRV GATATC 1 cut(s) 367
EcoT14I CCWWGG 2 cut(s) 535, 629
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 2 cut(s) 535, 629
Esp3I CGTCTC 1 cut(s) 534
FaeI CATG 8 cut(s) 391, 419, 453, 539, 584, 686, 739, 779
FaqI GGGAC 3 cut(s) 469, 717, 794
FatI CATG 8 cut(s) 387, 415, 449, 535, 580, 682, 735, 775
FauI CCCGC 1 cut(s) 560
FokI GGATG 2 cut(s) 72, 611
FriOI GRGCYC 1 cut(s) 104
FspBI CTAG 2 cut(s) 188, 575
GsaI CCCAGC 1 cut(s) 746
HaeIII GGCC 2 cut(s) 561, 740
HapII CCGG 4 cut(s) 306, 362, 563, 755
HgaI GACGC 1 cut(s) 626
Hin1II CATG 8 cut(s) 391, 419, 453, 539, 584, 686, 739, 779
HinfI GANTC 2 cut(s) 606, 795
HpaII CCGG 4 cut(s) 306, 362, 563, 755
HphI GGTGA 2 cut(s) 161, 542
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 3 cut(s) 232, 598, 818
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 1 cut(s) 303
Hpy99I CGWCG 3 cut(s) 239, 598, 620
HpyAV CCTTC 2 cut(s) 62, 707
HpyCH4III ACNGT 5 cut(s) 34, 200, 224, 328, 673
HpyCH4IV ACGT 2 cut(s) 473, 813
HpyCH4V TGCA 3 cut(s) 74, 682, 775
HpyF10VI GCNNNNNNNGC 3 cut(s) 425, 452, 737
HpyF3I CTNAG 1 cut(s) 718
HpySE526I ACGT 2 cut(s) 473, 813
Hsp92II CATG 8 cut(s) 391, 419, 453, 539, 584, 686, 739, 779
Kzo9I GATC 3 cut(s) 172, 392, 570
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 1 cut(s) 728
LweI GCATC 3 cut(s) 14, 136, 174
MabI ACCWGGT 2 cut(s) 279, 649
MaeI CTAG 2 cut(s) 188, 575
MaeII ACGT 2 cut(s) 473, 813
MaeIII GTNAC 5 cut(s) 34, 200, 491, 530, 760
MalI GATC 3 cut(s) 174, 394, 572
MboI GATC 3 cut(s) 172, 392, 570
MboII GAAGA 5 cut(s) 19, 96, 346, 523, 615
MflI RGATCY 1 cut(s) 570
MhlI GDGCHC 2 cut(s) 104, 696
MluCI AATT 2 cut(s) 286, 547
MmeI TCCRAC 1 cut(s) 576
MnlI CCTC 8 cut(s) 53, 74, 131, 253, 305, 357, 391, 718
MslI CAYNNNNRTG 3 cut(s) 147, 414, 528
MspI CCGG 4 cut(s) 306, 362, 563, 755
MspR9I CCNGG 6 cut(s) 281, 563, 564, 651, 755, 756
Mva1269I GAATGC 1 cut(s) 505
MvaI CCWGG 2 cut(s) 281, 651
MwoI GCNNNNNNNGC 3 cut(s) 425, 452, 737
NciI CCSGG 4 cut(s) 563, 564, 755, 756
NcoI CCATGG 1 cut(s) 535
NdeII GATC 3 cut(s) 172, 392, 570
NlaIII CATG 8 cut(s) 391, 419, 453, 539, 584, 686, 739, 779
NlaIV GGNNCC 5 cut(s) 379, 410, 572, 695, 741
NmuCI GTSAC 3 cut(s) 34, 530, 760
OliI CACNNNNGTG 1 cut(s) 147
PaeR7I CTCGAG 1 cut(s) 122
PciSI GCTCTTC 1 cut(s) 109
PctI GAATGC 1 cut(s) 505
PfeI GAWTC 2 cut(s) 606, 795
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 279, 649
PspEI GGTNACC 1 cut(s) 530
PspFI CCCAGC 1 cut(s) 742
PspGI CCWGG 2 cut(s) 279, 649
PspN4I GGNNCC 5 cut(s) 379, 410, 572, 695, 741
PspPI GGNCC 2 cut(s) 560, 739
PspXI VCTCGAGB 1 cut(s) 122
PsuI RGATCY 1 cut(s) 570
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 3 cut(s) 147, 414, 528
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 109
Sau3AI GATC 3 cut(s) 172, 392, 570
Sau96I GGNCC 2 cut(s) 560, 739
ScrFI CCNGG 6 cut(s) 281, 563, 564, 651, 755, 756
SduI GDGCHC 2 cut(s) 104, 696
SexAI ACCWGGT 2 cut(s) 279, 649
SfaNI GCATC 3 cut(s) 14, 136, 174
Sfr274I CTCGAG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 122
SmaI CCCGGG 2 cut(s) 564, 756
SmiMI CAYNNNNRTG 3 cut(s) 147, 414, 528
SmlI CTYRAG 3 cut(s) 122, 131, 296
SmoI CTYRAG 3 cut(s) 122, 131, 296
SrfI GCCCGGGC 1 cut(s) 564
Sse9I AATT 2 cut(s) 286, 547
SsiI CCGC 2 cut(s) 503, 567
SspMI CTAG 2 cut(s) 188, 575
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 6 cut(s) 279, 561, 562, 649, 753, 754
StyI CCWWGG 2 cut(s) 535, 629
TaaI ACNGT 5 cut(s) 34, 200, 224, 328, 673
TaiI ACGT 2 cut(s) 476, 816
TaqI TCGA 2 cut(s) 123, 332
TasI AATT 2 cut(s) 286, 547
TfiI GAWTC 2 cut(s) 606, 795
TscAI CASTG 4 cut(s) 37, 318, 333, 726
TseFI GTSAC 3 cut(s) 34, 530, 760
Tsp45I GTSAC 3 cut(s) 34, 530, 760
TspDTI ATGAA 3 cut(s) 512, 540, 699
TspMI CCCGGG 2 cut(s) 562, 754
TspRI CASTG 4 cut(s) 37, 318, 333, 726
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 1 cut(s) 547
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XhoI CTCGAG 1 cut(s) 122
XmaI CCCGGG 2 cut(s) 562, 754
XspI CTAG 2 cut(s) 188, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.