RLG00000021937

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000309
Physical Location & Seq
Reverse (-)
79416365 .. 79417492
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021937

Sequence Viewer

Length: 918 bp
ATGGCACCAAAATCTGATCTTTCTCTTTTCACAGTCACTCTCCTCCTCCTCCTCGCCATCTTTTCTTTCCTTTGTAATGCAACGGCAAACTCATCTCCGTTTGAGTTCCTCAAGCATCTTAAGGGTTGTCACAAAGGTGACAAGGTCAAGGGCATCAATGACCTCAAGAAGTACCTTTCAAAGTTTGGTTACTTAAACCACGAGAATCGCAGACATTCCGATGACGATGATTTTGACGAGCACTTGGAGGAAGCCGTCAAGACTTACCAGCTCAATTTCCACCTCAAGTCCACTGGAACATTGGACGACAAAACCATATCAACGATGATGATGCCTCGTTGTGGTGTGCCCGATATCATCAATGGCACCACATCCATGCGATCAGCCCAGATAAAGTGCCAGCACCGTGCAATTCATACCACTGTTCACTACTCATTTCCTGATGGAAATCTAAAATGGCCTTCTTCAAAATACCATCTGACTTACGGTTTTCTCGCCGGCACCCCATCAGAAGCCCCGGGCGCTGTCGCACAGGCTTTCTCAACATGGGCTAAGAACACTCACTTCAAATTCAGCAAGGCTCAAAATTACCAGAGTGCGGATTTGAAGGTTAGCTTTCATAAGGGTGATCATGGAGACGGGAATTCATTTGATGGGCCTGGCGGACAGCTGGCCCATGCTGCTTACCCTACGGAGGGAACATTGCACTACGACGCAGATGAGACTTGGTCTGTAGGTGCTAAACCAGGTGCCGTAGACATTGAGAGTGCTGGTTTGCATGAAATAGGGCACCTTCTGGGACTTGGACACAGCTCATTGGAGGAAGCTATCATGTATCCAACCATCCCCGAGGGAGTGGCTCATAAAAGTTTGCATGAGGACGATATTCAAGGAATAAAAGCTTTATACAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

306

Amino Acids

33.55

Weight (kDa)

6.27

Isoelectric Point (pI)

25.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 52 - 109 8e-11 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 152 - 303 1.6e-44 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 4, 365, 500, 749, 789
AccI GTMKAC 1 cut(s) 756
AciI CCGC 2 cut(s) 599, 663
AcsI RAATTY 2 cut(s) 569, 643
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 4 cut(s) 341, 598, 694, 695
AflII CTTAAG 1 cut(s) 119
AgsI TTSAA 5 cut(s) 180, 468, 568, 607, 890
AjnI CCWGG 2 cut(s) 658, 745
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 6 cut(s) 271, 615, 670, 813, 827, 902
AluI AGCT 6 cut(s) 271, 615, 670, 813, 827, 902
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 2 cut(s) 630, 716
Ama87I CYCGRG 2 cut(s) 517, 848
AoxI GGCC 3 cut(s) 458, 656, 672
ApeKI GCWGC 1 cut(s) 680
ApoI RAATTY 2 cut(s) 569, 643
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 2 cut(s) 656, 673
AsuC2I CCSGG 2 cut(s) 518, 519
AsuHPI GGTGA 2 cut(s) 149, 638
AvaI CYCGRG 2 cut(s) 517, 848
BaeGI GKGCMC 2 cut(s) 351, 792
BanI GGYRCC 5 cut(s) 4, 365, 500, 749, 789
BauI CACGAG 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 667
BccI CCATC 6 cut(s) 65, 437, 483, 514, 647, 851
BceAI ACGGC 3 cut(s) 99, 239, 737
BcgI CGANNNNNNTGC 2 cut(s) 313, 347
BciT130I CCWGG 2 cut(s) 660, 747
BciVI GTATCC 1 cut(s) 846
BclI TGATCA 1 cut(s) 628
BcnI CCSGG 2 cut(s) 518, 519
BcoDI GTCTC 2 cut(s) 630, 716
BfmI CTRYAG 1 cut(s) 732
BfoI RGCGCY 1 cut(s) 525
BfrI CTTAAG 1 cut(s) 119
BfuI GTATCC 1 cut(s) 846
BisI GCNGC 1 cut(s) 681
BlsI GCNGC 1 cut(s) 682
Bme1390I CCNGG 4 cut(s) 518, 519, 660, 747
BmeT110I CYCGRG 2 cut(s) 517, 848
BmgT120I GGNCC 2 cut(s) 656, 673
BmiI GGNNCC 5 cut(s) 6, 367, 502, 751, 791
BmrFI CCNGG 4 cut(s) 518, 519, 660, 747
BmsI GCATC 3 cut(s) 124, 162, 321
BpuEI CTTGAG 3 cut(s) 95, 149, 269
BpuMI CCSGG 2 cut(s) 518, 519
BsaBI GATNNNNATC 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 516, 517, 849
Bsc4I CCNNNNNNNGG 4 cut(s) 341, 598, 694, 695
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 1 cut(s) 298
Bse3DI GCAATG 1 cut(s) 701
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 2 cut(s) 660, 747
BseDI CCNNGG 3 cut(s) 516, 517, 849
BseGI GGATG 2 cut(s) 371, 843
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 4 cut(s) 341, 598, 694, 695
BseMI GCAATG 1 cut(s) 701
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 4 cut(s) 32, 35, 38, 41
BseSI GKGCMC 2 cut(s) 351, 792
BseXI GCAGC 1 cut(s) 667
BshFI GGCC 3 cut(s) 460, 658, 674
BshNI GGYRCC 5 cut(s) 4, 365, 500, 749, 789
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 2 cut(s) 517, 848
BsiSI CCGG 2 cut(s) 498, 518
BslFI GGGAC 1 cut(s) 813
BslI CCNNNNNNNGG 4 cut(s) 341, 598, 694, 695
BsmAI GTCTC 2 cut(s) 630, 716
BsmBI CGTCTC 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 813
BsnI GGCC 3 cut(s) 460, 658, 674
BsoBI CYCGRG 2 cut(s) 517, 848
Bsp1286I GDGCHC 3 cut(s) 243, 351, 792
Bsp143I GATC 3 cut(s) 16, 380, 628
BspACI CCGC 2 cut(s) 599, 663
BspANI GGCC 3 cut(s) 460, 658, 674
BspLI GGNNCC 5 cut(s) 6, 367, 502, 751, 791
BspT107I GGYRCC 5 cut(s) 4, 365, 500, 749, 789
BspTI CTTAAG 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 701
BsrFI RCCGGY 1 cut(s) 497
BsrI ACTGG 1 cut(s) 298
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 3 cut(s) 516, 517, 849
BssMI GATC 3 cut(s) 16, 380, 628
BssSI CACGAG 1 cut(s) 200
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 2 cut(s) 660, 747
Bst4CI ACNGT 4 cut(s) 34, 407, 424, 488
BstAFI CTTAAG 1 cut(s) 119
BstC8I GCNNGC 3 cut(s) 401, 499, 672
BstDEI CTNAG 1 cut(s) 552
BstF5I GGATG 2 cut(s) 371, 843
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 3 cut(s) 19, 383, 631
BstMAI GTCTC 2 cut(s) 630, 716
BstMBI GATC 3 cut(s) 16, 380, 628
BstMWI GCNNNNNNNGC 2 cut(s) 521, 680
BstNI CCWGG 2 cut(s) 660, 747
BstSCI CCNGG 4 cut(s) 516, 517, 658, 745
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 2 cut(s) 351, 792
BstV1I GCAGC 1 cut(s) 667
BsuI GTATCC 1 cut(s) 846
BsuRI GGCC 3 cut(s) 460, 658, 674
BtsCI GGATG 2 cut(s) 371, 843
BtsIMutI CAGTG 2 cut(s) 291, 420
Cac8I GCNNGC 3 cut(s) 401, 499, 672
CfoI GCGC 1 cut(s) 524
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 2 cut(s) 656, 673
Cfr9I CCCGGG 1 cut(s) 517
CseI GACGC 1 cut(s) 722
CsiI ACCWGGT 1 cut(s) 745
Csp6I GTAC 1 cut(s) 172
CviAII CATG 7 cut(s) 376, 546, 632, 677, 779, 832, 875
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 1 cut(s) 552
DpnI GATC 3 cut(s) 18, 382, 630
DpnII GATC 3 cut(s) 16, 380, 628
EciI GGCGGA 1 cut(s) 678
Eco32I GATATC 1 cut(s) 355
Eco88I CYCGRG 2 cut(s) 517, 848
EcoRI GAATTC 1 cut(s) 643
EcoRII CCWGG 2 cut(s) 658, 745
EcoRV GATATC 1 cut(s) 355
Esp3I CGTCTC 1 cut(s) 630
FaeI CATG 7 cut(s) 379, 549, 635, 680, 782, 835, 878
FalI AAGNNNNNCTT 2 cut(s) 599, 631
FaqI GGGAC 1 cut(s) 813
FatI CATG 7 cut(s) 375, 545, 631, 676, 778, 831, 874
FbaI TGATCA 1 cut(s) 628
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 1 cut(s) 681
FokI GGATG 2 cut(s) 358, 830
Fsp4HI GCNGC 1 cut(s) 681
GlaI GCGC 1 cut(s) 523
GluI GCNGC 1 cut(s) 681
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 3 cut(s) 460, 658, 674
HapII CCGG 2 cut(s) 498, 518
HgaI GACGC 1 cut(s) 722
HhaI GCGC 1 cut(s) 524
Hin1II CATG 7 cut(s) 379, 549, 635, 680, 782, 835, 878
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HindIII AAGCTT 1 cut(s) 900
HinfI GANTC 1 cut(s) 205
HpaII CCGG 2 cut(s) 498, 518
HphI GGTGA 2 cut(s) 149, 638
Hpy166II GTNNAC 3 cut(s) 291, 427, 757
Hpy188I TCNGA 4 cut(s) 16, 220, 480, 511
Hpy188III TCNNGA 3 cut(s) 166, 259, 440
Hpy8I GTNNAC 3 cut(s) 291, 427, 757
Hpy99I CGWCG 1 cut(s) 716
HpyAV CCTTC 3 cut(s) 471, 601, 803
HpyCH4III ACNGT 4 cut(s) 34, 407, 424, 488
HpyCH4V TGCA 5 cut(s) 80, 410, 706, 778, 874
HpyF10VI GCNNNNNNNGC 2 cut(s) 521, 680
HpyF3I CTNAG 1 cut(s) 552
Hsp92II CATG 7 cut(s) 379, 549, 635, 680, 782, 835, 878
HspAI GCGC 1 cut(s) 522
KroI GCCGGC 1 cut(s) 497
KroNI GCCGGC 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 628
Kzo9I GATC 3 cut(s) 16, 380, 628
Lsp1109I GCAGC 1 cut(s) 667
LweI GCATC 3 cut(s) 124, 162, 321
MabI ACCWGGT 1 cut(s) 745
MaeIII GTNAC 4 cut(s) 34, 128, 137, 188
MalI GATC 3 cut(s) 18, 382, 630
MboI GATC 3 cut(s) 16, 380, 628
MboII GAAGA 1 cut(s) 456
MhlI GDGCHC 3 cut(s) 243, 351, 792
MluCI AATT 5 cut(s) 274, 411, 569, 586, 643
MmeI TCCRAC 1 cut(s) 863
MroNI GCCGGC 1 cut(s) 497
MseI TTAA 2 cut(s) 120, 194
MslI CAYNNNNRTG 4 cut(s) 135, 219, 374, 624
MspA1I CMGCKG 1 cut(s) 670
MspCI CTTAAG 1 cut(s) 119
MspI CCGG 2 cut(s) 498, 518
MspR9I CCNGG 4 cut(s) 518, 519, 660, 747
MvaI CCWGG 2 cut(s) 660, 747
MwoI GCNNNNNNNGC 2 cut(s) 521, 680
NaeI GCCGGC 1 cut(s) 499
NciI CCSGG 2 cut(s) 518, 519
NdeII GATC 3 cut(s) 16, 380, 628
NgoMIV GCCGGC 1 cut(s) 497
NlaIII CATG 7 cut(s) 379, 549, 635, 680, 782, 835, 878
NlaIV GGNNCC 5 cut(s) 6, 367, 502, 751, 791
NmuCI GTSAC 3 cut(s) 34, 128, 137
OliI CACNNNNGTG 1 cut(s) 135
PdiI GCCGGC 1 cut(s) 499
PfeI GAWTC 1 cut(s) 205
PflFI GACNNNGTC 2 cut(s) 143, 727
PkrI GCNGC 1 cut(s) 682
Psp6I CCWGG 2 cut(s) 658, 745
PspGI CCWGG 2 cut(s) 658, 745
PspN4I GGNNCC 5 cut(s) 6, 367, 502, 751, 791
PspPI GGNCC 2 cut(s) 656, 673
PsyI GACNNNGTC 2 cut(s) 143, 727
PvuII CAGCTG 1 cut(s) 670
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 4 cut(s) 135, 219, 374, 624
SaqAI TTAA 2 cut(s) 120, 194
SatI GCNGC 1 cut(s) 681
Sau3AI GATC 3 cut(s) 16, 380, 628
Sau96I GGNCC 2 cut(s) 656, 673
ScrFI CCNGG 4 cut(s) 518, 519, 660, 747
SduI GDGCHC 3 cut(s) 243, 351, 792
SexAI ACCWGGT 1 cut(s) 745
SfaNI GCATC 3 cut(s) 124, 162, 321
SfcI CTRYAG 1 cut(s) 732
SmaI CCCGGG 1 cut(s) 519
SmiMI CAYNNNNRTG 4 cut(s) 135, 219, 374, 624
SmlI CTYRAG 4 cut(s) 110, 119, 164, 284
SmoI CTYRAG 4 cut(s) 110, 119, 164, 284
Sse9I AATT 5 cut(s) 274, 411, 569, 586, 643
SsiI CCGC 2 cut(s) 599, 663
StyD4I CCNGG 4 cut(s) 516, 517, 658, 745
TaaI ACNGT 4 cut(s) 34, 407, 424, 488
TasI AATT 5 cut(s) 274, 411, 569, 586, 643
TfiI GAWTC 1 cut(s) 205
Tru1I TTAA 2 cut(s) 120, 194
Tru9I TTAA 2 cut(s) 120, 194
TscAI CASTG 2 cut(s) 298, 427
TseFI GTSAC 3 cut(s) 34, 128, 137
TseI GCWGC 1 cut(s) 680
Tsp45I GTSAC 3 cut(s) 34, 128, 137
TspDTI ATGAA 4 cut(s) 404, 608, 636, 795
TspGWI ACGGA 2 cut(s) 87, 707
TspMI CCCGGG 1 cut(s) 517
TspRI CASTG 2 cut(s) 298, 427
Tth111I GACNNNGTC 2 cut(s) 143, 727
Vha464I CTTAAG 1 cut(s) 119
XapI RAATTY 2 cut(s) 569, 643
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XmaI CCCGGG 1 cut(s) 517
XmiI GTMKAC 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.