Rorug02G0547100

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000309
Physical Location & Seq
Forward (+)
67541101 .. 67543550
2450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0547100.1

Sequence Viewer

Length: 1719 bp
ATGGATCACTCTCACAAACCCAGAAAACCCAAATCCCGGGAAGTAAGCTCGCGCTTCCTATCTTCCCCTACACCCGAAACCACCACACTCCCATCTCCAAGCCACCCTCTCTCGCCGGCCCGGAGAAAACCCGGTTCGCCGATATCCGATGCCCGAAAACCCAAAACTCATCTCGACGATTCAAGCTCCATCCGAGGAGGAGGACTGTGGCCATCTTCAACAGCTAAGAACTTCGACTCTCTAGCAGTCCATCTCGGAATCGAGAGACTCAAAGACCGTATCGACCGTAAAAACTCGACCTCCGTCACAAGCCGGAGAGAAGCGAGTGTGTTTAATGAGAAAGACAACGCCAAAGAGAACCATAGACCGGTTCTCGGAGGGTCGGCGAGGTACACCGGGAAATTCAACTTTTCCGGGAAATCGTCGCCGTCGCCGTCGTCGTCTTCGACTTCAGTGTCTTCGAATAAACTATCGGCGGTGGTAGTTCCGGGGAGGTTTTCCGTTGACGGAAGTGGTCTGTATCAGAACTCAACTCGGCGAAGTTTCGATAGCTTCGCCGATGCTCTCGAGTCGGGATCGGAGTGCAGCGACGCCGGTTCCGGCAGGAATATCAATATCGGGTCGCCGAGTTTCGGGGCTCAGAGCTCGACGGCGAGTTCGAGAAAGTCGGGGGTGGAGGTTTCGTCTAAGTACATGAGTGATATTCAGACGAGGCATCATCGGAGATGGACGTCGGATTCTAATCTCCGGAATCCGATTACGGCAGAGAGCTCTCCGAAAATGAACAAGTTTAACCTAAAAAATGTGATCAAGAGGGCTAATTCGTTGACCGGGTCAAAGTTCGGGACGACGCAGTGGGCATTGTCGCCGGGAAGGAGCGCAGCGCAGCCTGTGTCGGCGGAGAGTTTGGGGAAGTCGACGTCATTTTCGAGCTTGAAGCCTCCGACTAGTCCTTCGAAAAGTAAGGGAGTGGAGAAGTTGCTCAATATGGGGTTTGACTTGTTCAAGAGTAAAAAATATTCTTCATCTACTAAGTCACTGGTGAGGTCTAATTCCGGGTATGGTGTGACCGATACCGGTCATCAACTCCGGATGCTTCAGAACAGATTGATGCAATGGCGGTACGCAAATGCAAGAGCTGAAATTGTGAATGGCAATGTTGCTGATCAAGCACAGGTCAATTTTTTGAGTGCTTGTAATAGTCTTATGAAGTTGCGGCAATCCGTGCTGCATAAGAAACTGAAGCTTCAGAAAGAAAGGCTCGAAATGAAGCTGAACTTTATACTACATTCTCAATTTGAGCCATTGGAAGCTTGGGGAGATATGGAAAGGCAACATATGGGAGCAGTTTCTATGTTGAAAGAGAGCTTACATTCTGTTGTGTGCAGAGTACCTCTAACTGAAGGTGCAAAGGTGGATACTCAATCAGCTGCCATCAATCTTCGAATTGCTTCAGATTACTCAGCCTCCATTAAGTTGATATTGGCTACATTTGCCCCCTCGGCTGAGCAGACTAGTGTGTTGGTCTCAGAATTAGCAGAGGTGGTTGCGCAAGAAAAGTTGCTTTCTGATGAGTGTCTTGAGCTCTGTAGAACCATATCAGAACTAGAGATGGAAGAGAGGGGACTGAAGTGTAGTATTATTCAATTGGAATCATGGAAACAAGAGCAAATTCAGCTGCAGCAGCAGCAATCAAGACAAGAAGATATCATGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

572

Amino Acids

62.82

Weight (kDa)

9.79

Isoelectric Point (pI)

57.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 215 - 522 3.9e-76 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 454
AatII GACGTC 2 cut(s) 734, 923
Acc16I TGCGCA 1 cut(s) 1551
AccI GTMKAC 1 cut(s) 917
AccII CGCG 1 cut(s) 52
AccIII TCCGGA 2 cut(s) 747, 1089
AciI CCGC 4 cut(s) 476, 899, 1120, 1216
AclWI GGATC 2 cut(s) 12, 583
AcoI YGGCCR 1 cut(s) 209
AcsI RAATTY 2 cut(s) 401, 1671
AcuI CTGAAG 7 cut(s) 435, 1082, 1232, 1262, 1422, 1437, 1649
AcyI GRCGYC 3 cut(s) 591, 731, 920
AfaI GTAC 4 cut(s) 392, 692, 1124, 1392
AfiI CCNNNNNNNGG 4 cut(s) 36, 367, 374, 632
AgeI ACCGGT 2 cut(s) 367, 1076
AgsI TTSAA 7 cut(s) 183, 219, 406, 937, 1006, 1360, 1646
AhlI ACTAGT 2 cut(s) 947, 1514
Alw21I GWGCWC 3 cut(s) 647, 773, 1587
Alw26I GTCTC 2 cut(s) 259, 1531
AlwI GGATC 2 cut(s) 12, 583
Ama87I CYCGRG 2 cut(s) 36, 566
Aor13HI TCCGGA 2 cut(s) 747, 1089
AoxI GGCC 2 cut(s) 117, 209
ApeKI GCWGC 9 cut(s) 585, 881, 886, 1228, 1430, 1678, 1681, 1684, 1687
ApoI RAATTY 2 cut(s) 401, 1671
ArsI GACNNNNNNTTYG 6 cut(s) 640, 672, 910, 937, 942, 969
AsiGI ACCGGT 2 cut(s) 367, 1076
Asp700I GAANNNNTTC 1 cut(s) 1450
AspLEI GCGC 4 cut(s) 54, 881, 886, 1552
AspS9I GGNCC 1 cut(s) 118
AsuHPI GGTGA 1 cut(s) 1054
AsuII TTCGAA 3 cut(s) 461, 956, 1444
AvaI CYCGRG 2 cut(s) 36, 566
BalI TGGCCA 1 cut(s) 211
BanII GRGCYC 4 cut(s) 640, 647, 773, 1587
BbsI GAAGAC 2 cut(s) 435, 450
Bbv12I GWGCWC 3 cut(s) 647, 773, 1587
BbvI GCAGC 9 cut(s) 597, 893, 898, 1215, 1417, 1665, 1693, 1696, 1699
BccI CCATC 7 cut(s) 100, 197, 220, 258, 720, 1442, 1606
BceAI ACGGC 4 cut(s) 412, 418, 666, 777
BciVI GTATCC 1 cut(s) 1411
BclI TGATCA 2 cut(s) 807, 1165
BcoDI GTCTC 2 cut(s) 259, 1531
BcuI ACTAGT 2 cut(s) 947, 1514
BfaI CTAG 4 cut(s) 242, 948, 1515, 1607
BfmI CTRYAG 2 cut(s) 1588, 1679
BfuI GTATCC 1 cut(s) 1411
BglI GCCNNNNNGGC 1 cut(s) 1502
BlpI GCTNAGC 1 cut(s) 1506
BmeT110I CYCGRG 2 cut(s) 36, 566
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 598
BmsI GCATC 5 cut(s) 139, 550, 724, 1083, 1101
BoxI GACNNNNGTC 1 cut(s) 302
BpiI GAAGAC 2 cut(s) 435, 450
Bpu1102I GCTNAGC 1 cut(s) 1506
Bpu14I TTCGAA 3 cut(s) 461, 956, 1444
BpuEI CTTGAG 1 cut(s) 1601
BsaBI GATNNNNATC 1 cut(s) 741
BsaHI GRCGYC 3 cut(s) 591, 731, 920
BsaI GGTCTC 1 cut(s) 1531
BsaJI CCNNGG 4 cut(s) 36, 193, 488, 1500
BsaWI WCCGGW 4 cut(s) 367, 747, 1076, 1089
BsaXI ACNNNNNCTCC 6 cut(s) 284, 314, 668, 698, 1451, 1481
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 367, 374, 632
Bse118I RCCGGY 4 cut(s) 115, 367, 593, 1076
Bse1I ACTGG 1 cut(s) 1044
Bse3DI GCAATG 2 cut(s) 1121, 1162
Bse8I GATNNNNATC 1 cut(s) 741
BseAI TCCGGA 2 cut(s) 747, 1089
BseDI CCNNGG 4 cut(s) 36, 193, 488, 1500
BseGI GGATG 2 cut(s) 189, 1098
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 4 cut(s) 36, 367, 374, 632
BseMI GCAATG 2 cut(s) 1121, 1162
BseMII CTCAG 4 cut(s) 653, 1476, 1497, 1542
BseNI ACTGG 1 cut(s) 1044
BseRI GAGGAG 2 cut(s) 210, 213
BseXI GCAGC 9 cut(s) 597, 893, 898, 1215, 1417, 1665, 1693, 1696, 1699
BsgI GTGCAG 2 cut(s) 604, 1405
Bsh1236I CGCG 1 cut(s) 52
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 2 cut(s) 119, 211
BshTI ACCGGT 2 cut(s) 367, 1076
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 3 cut(s) 647, 773, 1587
BsiHKCI CYCGRG 2 cut(s) 36, 566
BslFI GGGAC 2 cut(s) 859, 1638
BslI CCNNNNNNNGG 4 cut(s) 36, 367, 374, 632
BsmAI GTCTC 2 cut(s) 259, 1531
BsmFI GGGAC 2 cut(s) 859, 1638
BsnI GGCC 2 cut(s) 119, 211
Bso31I GGTCTC 1 cut(s) 1531
BsoBI CYCGRG 2 cut(s) 36, 566
Bsp119I TTCGAA 3 cut(s) 461, 956, 1444
Bsp1286I GDGCHC 4 cut(s) 640, 647, 773, 1587
Bsp13I TCCGGA 2 cut(s) 747, 1089
Bsp143I GATC 4 cut(s) 4, 575, 807, 1165
Bsp1720I GCTNAGC 1 cut(s) 1506
BspACI CCGC 4 cut(s) 476, 899, 1120, 1216
BspANI GGCC 2 cut(s) 119, 211
BspCNI CTCAG 4 cut(s) 652, 1475, 1498, 1541
BspEI TCCGGA 2 cut(s) 747, 1089
BspFNI CGCG 1 cut(s) 52
BspLI GGNNCC 1 cut(s) 598
BspMAI CTGCAG 1 cut(s) 1683
BspPI GGATC 2 cut(s) 12, 583
BspT104I TTCGAA 3 cut(s) 461, 956, 1444
BspTNI GGTCTC 1 cut(s) 1531
BsrDI GCAATG 2 cut(s) 1121, 1162
BsrFI RCCGGY 4 cut(s) 115, 367, 593, 1076
BsrI ACTGG 1 cut(s) 1044
BssAI RCCGGY 4 cut(s) 115, 367, 593, 1076
BssECI CCNNGG 4 cut(s) 36, 193, 488, 1500
BssMI GATC 4 cut(s) 4, 575, 807, 1165
BssNI GRCGYC 3 cut(s) 591, 731, 920
Bst4CI ACNGT 3 cut(s) 207, 278, 287
Bst6I CTCTTC 1 cut(s) 1611
BstACI GRCGYC 3 cut(s) 591, 731, 920
BstAPI GCANNNNNTGC 1 cut(s) 1225
BstBI TTCGAA 3 cut(s) 461, 956, 1444
BstC8I GCNNGC 2 cut(s) 50, 117
BstDEI CTNAG 7 cut(s) 225, 639, 687, 1032, 1462, 1506, 1528
BstF5I GGATG 2 cut(s) 189, 1098
BstFNI CGCG 1 cut(s) 52
BstHHI GCGC 4 cut(s) 54, 881, 886, 1552
BstKTI GATC 4 cut(s) 7, 578, 810, 1168
BstMAI GTCTC 2 cut(s) 259, 1531
BstMBI GATC 4 cut(s) 4, 575, 807, 1165
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 7 cut(s) 1169, 1225, 1493, 1502, 1675, 1684, 1687
BstPAI GACNNNNGTC 1 cut(s) 302
BstSFI CTRYAG 2 cut(s) 1588, 1679
BstUI CGCG 1 cut(s) 52
BstV1I GCAGC 9 cut(s) 597, 893, 898, 1215, 1417, 1665, 1693, 1696, 1699
BstV2I GAAGAC 2 cut(s) 435, 450
BsuI GTATCC 1 cut(s) 1411
BsuRI GGCC 2 cut(s) 119, 211
BtsCI GGATG 2 cut(s) 189, 1098
BtsI GCAGTG 1 cut(s) 860
BtsIMutI CAGTG 3 cut(s) 459, 860, 1037
Cac8I GCNNGC 2 cut(s) 50, 117
CfoI GCGC 4 cut(s) 54, 881, 886, 1552
Cfr10I RCCGGY 4 cut(s) 115, 367, 593, 1076
Cfr13I GGNCC 1 cut(s) 118
Cfr9I CCCGGG 1 cut(s) 36
CseI GACGC 2 cut(s) 599, 859
Csp6I GTAC 4 cut(s) 391, 691, 1123, 1391
CspAI ACCGGT 2 cut(s) 367, 1076
CviAII CATG 4 cut(s) 694, 1656, 1711, 1716
CviQI GTAC 4 cut(s) 391, 691, 1123, 1391
DdeI CTNAG 7 cut(s) 225, 639, 687, 1032, 1462, 1506, 1528
DpnI GATC 4 cut(s) 6, 577, 809, 1167
DpnII GATC 4 cut(s) 4, 575, 807, 1165
DrdI GACNNNNNNGTC 1 cut(s) 454
DseDI GACNNNNNNGTC 1 cut(s) 454
EaeI YGGCCR 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 1611
EarI CTCTTC 1 cut(s) 1611
EciI GGCGGA 1 cut(s) 914
Ecl136II GAGCTC 3 cut(s) 645, 771, 1585
Eco24I GRGCYC 4 cut(s) 640, 647, 773, 1587
Eco31I GGTCTC 1 cut(s) 1531
Eco32I GATATC 2 cut(s) 144, 1708
Eco53kI GAGCTC 3 cut(s) 645, 771, 1585
Eco57I CTGAAG 7 cut(s) 435, 1082, 1232, 1262, 1422, 1437, 1649
Eco88I CYCGRG 2 cut(s) 36, 566
EcoICRI GAGCTC 3 cut(s) 645, 771, 1585
EcoRV GATATC 2 cut(s) 144, 1708
EcoT38I GRGCYC 4 cut(s) 640, 647, 773, 1587
FaeI CATG 4 cut(s) 697, 1659, 1714, 1719
FalI AAGNNNNNCTT 2 cut(s) 1262, 1294
FaqI GGGAC 2 cut(s) 859, 1638
FatI CATG 4 cut(s) 693, 1655, 1710, 1715
FauNDI CATATG 1 cut(s) 1338
FbaI TGATCA 2 cut(s) 807, 1165
FblI GTMKAC 1 cut(s) 917
FokI GGATG 2 cut(s) 176, 1105
FriOI GRGCYC 4 cut(s) 640, 647, 773, 1587
FspBI CTAG 4 cut(s) 242, 948, 1515, 1607
FspI TGCGCA 1 cut(s) 1551
GlaI GCGC 4 cut(s) 53, 880, 885, 1551
HaeIII GGCC 2 cut(s) 119, 211
HgaI GACGC 2 cut(s) 599, 859
HhaI GCGC 4 cut(s) 54, 881, 886, 1552
Hin1I GRCGYC 3 cut(s) 591, 731, 920
Hin1II CATG 4 cut(s) 697, 1659, 1714, 1719
Hin6I GCGC 4 cut(s) 52, 879, 884, 1550
HinP1I GCGC 4 cut(s) 52, 879, 884, 1550
HincII GTYRAC 3 cut(s) 505, 828, 918
HindII GTYRAC 3 cut(s) 505, 828, 918
HindIII AAGCTT 2 cut(s) 1244, 1311
HinfI GANTC 8 cut(s) 179, 236, 258, 267, 569, 737, 751, 1652
HphI GGTGA 1 cut(s) 1054
Hpy166II GTNNAC 4 cut(s) 393, 505, 828, 918
Hpy8I GTNNAC 4 cut(s) 393, 505, 828, 918
HpyAV CCTTC 3 cut(s) 867, 963, 1397
HpyCH4III ACNGT 3 cut(s) 207, 278, 287
HpyCH4IV ACGT 2 cut(s) 731, 920
HpyCH4V TGCA 7 cut(s) 585, 1114, 1133, 1231, 1386, 1409, 1681
HpyF10VI GCNNNNNNNGC 7 cut(s) 1169, 1225, 1493, 1502, 1675, 1684, 1687
HpyF3I CTNAG 7 cut(s) 225, 639, 687, 1032, 1462, 1506, 1528
HpySE526I ACGT 2 cut(s) 731, 920
Hsp92I GRCGYC 3 cut(s) 591, 731, 920
Hsp92II CATG 4 cut(s) 697, 1659, 1714, 1719
HspAI GCGC 4 cut(s) 52, 879, 884, 1550
Kpn2I TCCGGA 2 cut(s) 747, 1089
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Ksp22I TGATCA 2 cut(s) 807, 1165
Kzo9I GATC 4 cut(s) 4, 575, 807, 1165
LmnI GCTCC 3 cut(s) 191, 876, 1343
Lsp1109I GCAGC 9 cut(s) 597, 893, 898, 1215, 1417, 1665, 1693, 1696, 1699
LweI GCATC 5 cut(s) 139, 550, 724, 1083, 1101
MaeI CTAG 4 cut(s) 242, 948, 1515, 1607
MaeII ACGT 2 cut(s) 731, 920
MaeIII GTNAC 3 cut(s) 304, 1035, 1066
MalI GATC 4 cut(s) 6, 577, 809, 1167
MboI GATC 4 cut(s) 4, 575, 807, 1165
MboII GAAGA 8 cut(s) 54, 207, 435, 450, 1014, 1433, 1628, 1715
MfeI CAATTG 1 cut(s) 1646
MhlI GDGCHC 4 cut(s) 640, 647, 773, 1587
MlsI TGGCCA 1 cut(s) 211
MluNI TGGCCA 1 cut(s) 211
MlyI GAGTC 3 cut(s) 230, 261, 578
MmeI TCCRAC 2 cut(s) 714, 968
Mox20I TGGCCA 1 cut(s) 211
MroI TCCGGA 2 cut(s) 747, 1089
MroNI GCCGGC 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 1450
MscI TGGCCA 1 cut(s) 211
MseI TTAA 3 cut(s) 333, 792, 1473
Msp20I TGGCCA 1 cut(s) 211
MspA1I CMGCKG 2 cut(s) 1430, 1678
MteI GCGCNGCGC 1 cut(s) 882
MunI CAATTG 1 cut(s) 1646
MvnI CGCG 1 cut(s) 52
MwoI GCNNNNNNNGC 7 cut(s) 1169, 1225, 1493, 1502, 1675, 1684, 1687
NaeI GCCGGC 1 cut(s) 117
NdeI CATATG 1 cut(s) 1338
NdeII GATC 4 cut(s) 4, 575, 807, 1165
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 4 cut(s) 697, 1659, 1714, 1719
NlaIV GGNNCC 1 cut(s) 598
NmeAIII GCCGAG 3 cut(s) 514, 651, 1481
NmuCI GTSAC 3 cut(s) 304, 1035, 1066
NsbI TGCGCA 1 cut(s) 1551
NspV TTCGAA 3 cut(s) 461, 956, 1444
PaeR7I CTCGAG 1 cut(s) 566
PcsI WCGNNNNNNNCGW 5 cut(s) 431, 437, 443, 656, 926
PdiI GCCGGC 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 1450
PfeI GAWTC 5 cut(s) 179, 258, 737, 751, 1652
PflFI GACNNNGTC 1 cut(s) 832
PfoI TCCNGGA 1 cut(s) 413
PinAI ACCGGT 2 cut(s) 367, 1076
PleI GAGTC 3 cut(s) 230, 261, 577
PpsI GAGTC 3 cut(s) 230, 261, 577
PshAI GACNNNNGTC 1 cut(s) 302
Psp124BI GAGCTC 3 cut(s) 647, 773, 1587
PspN4I GGNNCC 1 cut(s) 598
PspPI GGNCC 1 cut(s) 118
PstI CTGCAG 1 cut(s) 1683
PsyI GACNNNGTC 1 cut(s) 832
PvuII CAGCTG 2 cut(s) 1430, 1678
RsaI GTAC 4 cut(s) 392, 692, 1124, 1392
RsaNI GTAC 4 cut(s) 391, 691, 1123, 1391
SacI GAGCTC 3 cut(s) 647, 773, 1587
SalI GTCGAC 1 cut(s) 916
SaqAI TTAA 3 cut(s) 333, 792, 1473
Sau3AI GATC 4 cut(s) 4, 575, 807, 1165
Sau96I GGNCC 1 cut(s) 118
SchI GAGTC 3 cut(s) 230, 261, 578
SduI GDGCHC 4 cut(s) 640, 647, 773, 1587
SfaNI GCATC 5 cut(s) 139, 550, 724, 1083, 1101
SfcI CTRYAG 2 cut(s) 1588, 1679
Sfr274I CTCGAG 1 cut(s) 566
SfuI TTCGAA 3 cut(s) 461, 956, 1444
SlaI CTCGAG 1 cut(s) 566
SmaI CCCGGG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 566, 1580
SmoI CTYRAG 2 cut(s) 566, 1580
SpeI ACTAGT 2 cut(s) 947, 1514
SsiI CCGC 4 cut(s) 476, 899, 1120, 1216
SspI AATATT 1 cut(s) 1019
SspMI CTAG 4 cut(s) 242, 948, 1515, 1607
SstI GAGCTC 3 cut(s) 647, 773, 1587
TaaI ACNGT 3 cut(s) 207, 278, 287
TaiI ACGT 2 cut(s) 734, 923
TaqII GACCGA 1 cut(s) 1085
TatI WGTACW 1 cut(s) 690
TauI GCSGC 1 cut(s) 1219
TfiI GAWTC 5 cut(s) 179, 258, 737, 751, 1652
Tru1I TTAA 3 cut(s) 333, 792, 1473
Tru9I TTAA 3 cut(s) 333, 792, 1473
TscAI CASTG 3 cut(s) 459, 860, 1044
TseFI GTSAC 3 cut(s) 304, 1035, 1066
TseI GCWGC 9 cut(s) 585, 881, 886, 1228, 1430, 1678, 1681, 1684, 1687
Tsp45I GTSAC 3 cut(s) 304, 1035, 1066
TspDTI ATGAA 4 cut(s) 797, 1014, 1223, 1283
TspGWI ACGGA 4 cut(s) 292, 490, 522, 1213
TspMI CCCGGG 1 cut(s) 36
TspRI CASTG 3 cut(s) 459, 860, 1044
Tth111I GACNNNGTC 1 cut(s) 832
XapI RAATTY 2 cut(s) 401, 1671
XcmI CCANNNNNNNNNTGG 1 cut(s) 1311
XhoI CTCGAG 1 cut(s) 566
XmaI CCCGGG 1 cut(s) 36
XmiI GTMKAC 1 cut(s) 917
XmnI GAANNNNTTC 1 cut(s) 1450
XspI CTAG 4 cut(s) 242, 948, 1515, 1607
ZraI GACGTC 2 cut(s) 732, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.