MD04G1045600.v1.1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000309
Physical Location & Seq
Forward (+)
5301408 .. 5302337
930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1045600.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGCCTTAAAAGCCCTCTCTCTCATCCTTTTCTCTCTTCTCTCTTCTGCAACTTCATCATCTTCTCCGTTTGAGTTCTTAGACCATCTGAGGGGGGGTCACAAGGGTGACCACATCGAAGGCATCCATGACCTCAAGCTATACCTCGAAAAATTCGGCTACTTAAGCTATGCCCATGCCAAGAACAACCCTCATGCTAATGACGATGATTTTGACAACCTACTCGAGTTAGCCGTCAAGACATACCAGCTCAATTACCACCTCAACACCACCGGAACATTGGATGGCGAAACGGTGTCAAAGATGATGATGCCTCGTTGTGGTGTGGCGGATATCATCAATGGCACCAGCTCAATGAGATCAGGGAAGAAAAAGCACCATCACCATGACCCTGACCACGACCACCACAACCACGGTTCCAGTGTGCATGCAGTCTCTCACTATTCTTTCTTCAAGGGAAGGCTAAGATGGCCAACTTCTAAGCATCATCTCACATATGGATTTCTTCCAAACACCCCGACAGAAGCCATGGGACCGGTTTCATATGCGTTTCGAACATGGGCGTCAAACACGCACTTCAGATTTAGTCGTTCCAGGAACTACAGAAGGGCGGACATCAAGATTAGTTTCGAATACGGCGATCATGGAGATGGGAGCCCATTTGATGGGGAAGGTGGGGTTTTGGCCCATGCTTATGCTCCAACAAATGGAAGATTCCACTACGACGCCGACGAGCCATGGTCTGTGGGTGCTTTGGAAGGTTATTACGACTTGGAGACGGTGGCTTTGCATGAGATTGGACACCTTCTTGGACTAGGGCATAGCTCAGTTGAGGGAGCCATCATGTACCCTTACATATCTTCTGGAGCCACCAAGCTGACTTTGCATGACGACGACATTCAAGGGATTAAAGTTCTATACAAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

310

Amino Acids

34.5

Weight (kDa)

6.44

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 41 - 102 1.4e-09 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 157 - 307 2.5e-48 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 344
AccB7I CCANNNNNTGG 2 cut(s) 665, 707
AciI CCGC 2 cut(s) 329, 611
AcoI YGGCCR 1 cut(s) 470
AcsI RAATTY 1 cut(s) 152
AcuI CTGAAG 1 cut(s) 562
AcyI GRCGYC 2 cut(s) 563, 726
AfaI GTAC 1 cut(s) 848
AfiI CCNNNNNNNGG 4 cut(s) 91, 320, 665, 707
AflII CTTAAG 1 cut(s) 163
AgeI ACCGGT 1 cut(s) 535
AgsI TTSAA 2 cut(s) 454, 902
AjnI CCWGG 1 cut(s) 593
AleI CACNNNNGTG 1 cut(s) 105
AluBI AGCT 6 cut(s) 139, 168, 250, 351, 825, 877
AluI AGCT 6 cut(s) 139, 168, 250, 351, 825, 877
Alw26I GTCTC 2 cut(s) 439, 770
Ama87I CYCGRG 1 cut(s) 224
AoxI GGCC 3 cut(s) 3, 470, 684
ApoI RAATTY 1 cut(s) 152
ArsI GACNNNNNNTTYG 4 cut(s) 194, 226, 769, 801
AsiGI ACCGGT 1 cut(s) 535
AspS9I GGNCC 2 cut(s) 533, 685
AsuHPI GGTGA 2 cut(s) 119, 374
AsuII TTCGAA 2 cut(s) 553, 630
AvaI CYCGRG 1 cut(s) 224
AvaII GGWCC 1 cut(s) 533
BalI TGGCCA 1 cut(s) 472
BanI GGYRCC 1 cut(s) 344
BanII GRGCYC 1 cut(s) 659
BccI CCATC 7 cut(s) 93, 278, 387, 462, 644, 659, 848
BceAI ACGGC 2 cut(s) 218, 652
BciT130I CCWGG 1 cut(s) 595
BcoDI GTCTC 2 cut(s) 439, 770
BfaI CTAG 1 cut(s) 815
BfmI CTRYAG 1 cut(s) 601
BfrI CTTAAG 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 595
Bme18I GGWCC 1 cut(s) 533
BmeT110I CYCGRG 1 cut(s) 224
BmgT120I GGNCC 2 cut(s) 533, 685
BmiI GGNNCC 6 cut(s) 346, 418, 534, 656, 838, 868
BmrFI CCNGG 1 cut(s) 595
BmsI GCATC 3 cut(s) 132, 300, 493
BpmI CTGGAG 1 cut(s) 885
Bpu14I TTCGAA 2 cut(s) 553, 630
BpuEI CTTGAG 1 cut(s) 119
BsaHI GRCGYC 2 cut(s) 563, 726
BsaJI CCNNGG 3 cut(s) 412, 528, 737
BsaWI WCCGGW 2 cut(s) 272, 535
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 320, 665, 707
Bse118I RCCGGY 1 cut(s) 535
Bse1I ACTGG 1 cut(s) 420
BseBI CCWGG 1 cut(s) 595
BseDI CCNNGG 3 cut(s) 412, 528, 737
BseGI GGATG 3 cut(s) 24, 123, 289
BseLI CCNNNNNNNGG 4 cut(s) 91, 320, 665, 707
BseMII CTCAG 2 cut(s) 80, 840
BseNI ACTGG 1 cut(s) 420
BshFI GGCC 3 cut(s) 5, 472, 686
BshNI GGYRCC 1 cut(s) 344
BshTI ACCGGT 1 cut(s) 535
BsiHKCI CYCGRG 1 cut(s) 224
BsiSI CCGG 2 cut(s) 273, 536
BslFI GGGAC 1 cut(s) 546
BslI CCNNNNNNNGG 4 cut(s) 91, 320, 665, 707
BsmAI GTCTC 2 cut(s) 439, 770
BsmBI CGTCTC 1 cut(s) 770
BsmFI GGGAC 1 cut(s) 546
BsnI GGCC 3 cut(s) 5, 472, 686
BsoBI CYCGRG 1 cut(s) 224
Bsp119I TTCGAA 2 cut(s) 553, 630
Bsp1286I GDGCHC 1 cut(s) 659
Bsp143I GATC 2 cut(s) 359, 640
Bsp19I CCATGG 2 cut(s) 528, 737
BspACI CCGC 2 cut(s) 329, 611
BspANI GGCC 3 cut(s) 5, 472, 686
BspCNI CTCAG 2 cut(s) 81, 839
BspLI GGNNCC 6 cut(s) 346, 418, 534, 656, 838, 868
BspT104I TTCGAA 2 cut(s) 553, 630
BspT107I GGYRCC 1 cut(s) 344
BspTI CTTAAG 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 535
BsrI ACTGG 1 cut(s) 420
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 3 cut(s) 412, 528, 737
BssMI GATC 2 cut(s) 359, 640
BssNI GRCGYC 2 cut(s) 563, 726
BssT1I CCWWGG 2 cut(s) 528, 737
Bst2UI CCWGG 1 cut(s) 595
Bst4CI ACNGT 3 cut(s) 295, 416, 781
Bst6I CTCTTC 2 cut(s) 42, 49
BstACI GRCGYC 2 cut(s) 563, 726
BstAFI CTTAAG 1 cut(s) 163
BstBI TTCGAA 2 cut(s) 553, 630
BstC8I GCNNGC 1 cut(s) 429
BstDEI CTNAG 5 cut(s) 79, 89, 464, 480, 826
BstDSI CCRYGG 3 cut(s) 412, 528, 737
BstEII GGTNACC 1 cut(s) 107
BstF5I GGATG 3 cut(s) 24, 123, 289
BstKTI GATC 2 cut(s) 362, 643
BstMAI GTCTC 2 cut(s) 439, 770
BstMBI GATC 2 cut(s) 359, 640
BstMWI GCNNNNNNNGC 4 cut(s) 11, 165, 469, 883
BstNI CCWGG 1 cut(s) 595
BstNSI RCATGY 1 cut(s) 431
BstPI GGTNACC 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 593
BstSFI CTRYAG 1 cut(s) 601
BsuRI GGCC 3 cut(s) 5, 472, 686
BtgI CCRYGG 3 cut(s) 412, 528, 737
BtsCI GGATG 3 cut(s) 24, 123, 289
BtsIMutI CAGTG 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 429
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 2 cut(s) 533, 685
CseI GACGC 2 cut(s) 552, 734
Csp6I GTAC 1 cut(s) 847
CspAI ACCGGT 1 cut(s) 535
CviQI GTAC 1 cut(s) 847
DdeI CTNAG 5 cut(s) 79, 89, 464, 480, 826
DpnI GATC 2 cut(s) 361, 642
DpnII GATC 2 cut(s) 359, 640
EaeI YGGCCR 1 cut(s) 470
Eam1104I CTCTTC 2 cut(s) 42, 49
EarI CTCTTC 2 cut(s) 42, 49
EciI GGCGGA 2 cut(s) 344, 626
Eco130I CCWWGG 2 cut(s) 528, 737
Eco24I GRGCYC 1 cut(s) 659
Eco32I GATATC 1 cut(s) 334
Eco47I GGWCC 1 cut(s) 533
Eco57I CTGAAG 1 cut(s) 562
Eco88I CYCGRG 1 cut(s) 224
Eco91I GGTNACC 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 593
EcoRV GATATC 1 cut(s) 334
EcoT14I CCWWGG 2 cut(s) 528, 737
EcoT38I GRGCYC 1 cut(s) 659
ErhI CCWWGG 2 cut(s) 528, 737
Esp3I CGTCTC 1 cut(s) 770
FaqI GGGAC 1 cut(s) 546
FauNDI CATATG 2 cut(s) 496, 544
FokI GGATG 3 cut(s) 11, 110, 296
FriOI GRGCYC 1 cut(s) 659
FspBI CTAG 1 cut(s) 815
GsuI CTGGAG 1 cut(s) 885
HaeIII GGCC 3 cut(s) 5, 472, 686
HapII CCGG 2 cut(s) 273, 536
HgaI GACGC 2 cut(s) 552, 734
Hin1I GRCGYC 2 cut(s) 563, 726
HinfI GANTC 1 cut(s) 714
HpaII CCGG 2 cut(s) 273, 536
HphI GGTGA 2 cut(s) 119, 374
Hpy188I TCNGA 2 cut(s) 90, 581
Hpy188III TCNNGA 4 cut(s) 238, 619, 864, 927
Hpy99I CGWCG 3 cut(s) 728, 734, 896
HpyAV CCTTC 6 cut(s) 113, 453, 600, 665, 752, 815
HpyCH4III ACNGT 3 cut(s) 295, 416, 781
HpyCH4V TGCA 5 cut(s) 50, 427, 431, 790, 886
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 165, 469, 883
HpyF3I CTNAG 5 cut(s) 79, 89, 464, 480, 826
Hsp92I GRCGYC 2 cut(s) 563, 726
Kzo9I GATC 2 cut(s) 359, 640
LmnI GCTCC 4 cut(s) 654, 703, 836, 866
LweI GCATC 3 cut(s) 132, 300, 493
MaeI CTAG 1 cut(s) 815
MaeIII GTNAC 2 cut(s) 98, 107
MalI GATC 2 cut(s) 361, 642
MboI GATC 2 cut(s) 359, 640
MboII GAAGA 8 cut(s) 29, 36, 54, 379, 442, 497, 723, 852
MhlI GDGCHC 1 cut(s) 659
MlsI TGGCCA 1 cut(s) 472
MluCI AATT 2 cut(s) 152, 253
MluNI TGGCCA 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 725
MnlI CCTC 8 cut(s) 26, 84, 143, 155, 201, 272, 324, 826
Mox20I TGGCCA 1 cut(s) 472
MscI TGGCCA 1 cut(s) 472
MseI TTAA 3 cut(s) 8, 164, 909
MslI CAYNNNNRTG 5 cut(s) 105, 198, 384, 648, 693
Msp20I TGGCCA 1 cut(s) 472
MspCI CTTAAG 1 cut(s) 163
MspI CCGG 2 cut(s) 273, 536
MspR9I CCNGG 1 cut(s) 595
MvaI CCWGG 1 cut(s) 595
MwoI GCNNNNNNNGC 4 cut(s) 11, 165, 469, 883
NcoI CCATGG 2 cut(s) 528, 737
NdeI CATATG 2 cut(s) 496, 544
NdeII GATC 2 cut(s) 359, 640
NlaIV GGNNCC 6 cut(s) 346, 418, 534, 656, 838, 868
NmuCI GTSAC 2 cut(s) 98, 107
NspI RCATGY 1 cut(s) 431
NspV TTCGAA 2 cut(s) 553, 630
OliI CACNNNNGTG 1 cut(s) 105
PaeI GCATGC 1 cut(s) 431
PaeR7I CTCGAG 1 cut(s) 224
PcsI WCGNNNNNNNCGW 3 cut(s) 231, 636, 729
PfeI GAWTC 1 cut(s) 714
PflMI CCANNNNNTGG 2 cut(s) 665, 707
PfoI TCCNGGA 1 cut(s) 593
PinAI ACCGGT 1 cut(s) 535
Psp6I CCWGG 1 cut(s) 593
PspEI GGTNACC 1 cut(s) 107
PspGI CCWGG 1 cut(s) 593
PspN4I GGNNCC 6 cut(s) 346, 418, 534, 656, 838, 868
PspPI GGNCC 2 cut(s) 533, 685
PspXI VCTCGAGB 1 cut(s) 224
RsaI GTAC 1 cut(s) 848
RsaNI GTAC 1 cut(s) 847
RseI CAYNNNNRTG 5 cut(s) 105, 198, 384, 648, 693
SaqAI TTAA 3 cut(s) 8, 164, 909
Sau3AI GATC 2 cut(s) 359, 640
Sau96I GGNCC 2 cut(s) 533, 685
ScrFI CCNGG 1 cut(s) 595
SduI GDGCHC 1 cut(s) 659
SfaNI GCATC 3 cut(s) 132, 300, 493
SfcI CTRYAG 1 cut(s) 601
Sfr274I CTCGAG 1 cut(s) 224
SfuI TTCGAA 2 cut(s) 553, 630
SinI GGWCC 1 cut(s) 533
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 5 cut(s) 105, 198, 384, 648, 693
SmlI CTYRAG 3 cut(s) 134, 163, 224
SmoI CTYRAG 3 cut(s) 134, 163, 224
SphI GCATGC 1 cut(s) 431
Sse9I AATT 2 cut(s) 152, 253
SsiI CCGC 2 cut(s) 329, 611
SspMI CTAG 1 cut(s) 815
StyD4I CCNGG 1 cut(s) 593
StyI CCWWGG 2 cut(s) 528, 737
TaaI ACNGT 3 cut(s) 295, 416, 781
TaqI TCGA 5 cut(s) 117, 147, 225, 553, 630
TasI AATT 2 cut(s) 152, 253
TfiI GAWTC 1 cut(s) 714
Tru1I TTAA 3 cut(s) 8, 164, 909
Tru9I TTAA 3 cut(s) 8, 164, 909
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 2 cut(s) 98, 107
Tsp45I GTSAC 2 cut(s) 98, 107
TspDTI ATGAA 2 cut(s) 45, 531
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 1 cut(s) 427
Van91I CCANNNNNTGG 2 cut(s) 665, 707
Vha464I CTTAAG 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 533
XapI RAATTY 1 cut(s) 152
XceI RCATGY 1 cut(s) 431
XcmI CCANNNNNNNNNTGG 1 cut(s) 277
XhoI CTCGAG 1 cut(s) 224
XspI CTAG 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.