Rh2DG642300

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000309
Physical Location & Seq
Reverse (-)
86918077 .. 86921701
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG642300.1

Sequence Viewer

Length: 528 bp
ATGGAGACGGGAATTCATTTGATGGGCCTGGCGGACAGCTGGCCCATGCTGCTTACCCTACGGAGGGAACATTGCACTACGACGCAGATGAGACTTGGTCTTTTTCTCCCCAACACCCCATCCGAAGCCACGGGCGCTGTCGCACCGGCTTTCGCAACATGGGCTGGAAACACTCATTTCACGTTCAGCAAGTCCCAAAGTTACGAGAATGCGAATTTGAAGATTAGTTTTCGTAAGAGTGATCATGGAGACGGGAATTCATTTGACGGCCCGGGAGGAGTCCTAGCCCATGCTTTTAGCCCGTCAGATGGAAGATTCCACTATGACGCAGATGAGCCTTGGTCTGTGGGTGCTACACCAGGTGCTTATGATTTGGAGACTGTGGCTTTGCATGAAATAGGGCACCTTCTGGGACTTGGACATAGCTCAGTGGAGGGAGCTATCATGGCCCCAGCTATTCCCCCAGGAGTGACTAAAAGTTTTCATGGGGACGATTTTCAAGGAATCAAGGCTTTATACAACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

175

Amino Acids

18.73

Weight (kDa)

5.57

Isoelectric Point (pI)

31.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 41 - 173 3.7e-42 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 402
AciI CCGC 1 cut(s) 32
AcsI RAATTY 3 cut(s) 12, 214, 256
AdeI CACNNNGTG 1 cut(s) 362
AfiI CCNNNNNNNGG 3 cut(s) 63, 64, 308
AgsI TTSAA 2 cut(s) 220, 500
AjnI CCWGG 3 cut(s) 27, 358, 463
AluBI AGCT 4 cut(s) 39, 426, 440, 455
AluI AGCT 4 cut(s) 39, 426, 440, 455
Alw26I GTCTC 3 cut(s) 85, 243, 371
Ama87I CYCGRG 1 cut(s) 271
AoxI GGCC 4 cut(s) 25, 41, 268, 447
ApeKI GCWGC 1 cut(s) 49
ApoI RAATTY 3 cut(s) 12, 214, 256
ArsI GACNNNNNNTTYG 2 cut(s) 370, 402
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 4 cut(s) 25, 42, 269, 448
AsuC2I CCSGG 2 cut(s) 272, 273
AvaI CYCGRG 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 405
BanI GGYRCC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 36
BccI CCATC 3 cut(s) 16, 127, 302
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 3 cut(s) 29, 360, 465
BclI TGATCA 1 cut(s) 241
BcnI CCSGG 2 cut(s) 272, 273
BcoDI GTCTC 3 cut(s) 85, 243, 371
BfaI CTAG 1 cut(s) 284
BfoI RGCGCY 1 cut(s) 138
BisI GCNGC 1 cut(s) 50
BlsI GCNGC 1 cut(s) 51
Bme1390I CCNGG 5 cut(s) 29, 272, 273, 360, 465
BmeT110I CYCGRG 1 cut(s) 271
BmgT120I GGNCC 4 cut(s) 25, 42, 269, 448
BmiI GGNNCC 2 cut(s) 404, 450
BmrFI CCNGG 5 cut(s) 29, 272, 273, 360, 465
BpuMI CCSGG 2 cut(s) 272, 273
BsaJI CCNNGG 4 cut(s) 129, 271, 338, 463
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 64, 308
Bse118I RCCGGY 1 cut(s) 145
Bse3DI GCAATG 1 cut(s) 70
BseBI CCWGG 3 cut(s) 29, 360, 465
BseDI CCNNGG 4 cut(s) 129, 271, 338, 463
BseGI GGATG 1 cut(s) 119
BseLI CCNNNNNNNGG 3 cut(s) 63, 64, 308
BseMI GCAATG 1 cut(s) 70
BseMII CTCAG 1 cut(s) 441
BseRI GAGGAG 1 cut(s) 291
BseSI GKGCMC 1 cut(s) 405
BseXI GCAGC 1 cut(s) 36
BseYI CCCAGC 1 cut(s) 451
BshFI GGCC 4 cut(s) 27, 43, 270, 449
BshNI GGYRCC 1 cut(s) 402
BsiHKCI CYCGRG 1 cut(s) 271
BsiSI CCGG 2 cut(s) 146, 272
BslFI GGGAC 3 cut(s) 178, 426, 503
BslI CCNNNNNNNGG 3 cut(s) 63, 64, 308
BsmAI GTCTC 3 cut(s) 85, 243, 371
BsmBI CGTCTC 1 cut(s) 243
BsmFI GGGAC 3 cut(s) 178, 426, 503
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 4 cut(s) 27, 43, 270, 449
BsoBI CYCGRG 1 cut(s) 271
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 1 cut(s) 241
BspACI CCGC 1 cut(s) 32
BspANI GGCC 4 cut(s) 27, 43, 270, 449
BspCNI CTCAG 1 cut(s) 440
BspLI GGNNCC 2 cut(s) 404, 450
BspT107I GGYRCC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 4 cut(s) 129, 271, 338, 463
BssMI GATC 1 cut(s) 241
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 3 cut(s) 29, 360, 465
Bst4CI ACNGT 1 cut(s) 382
BstC8I GCNNGC 1 cut(s) 41
BstDEI CTNAG 1 cut(s) 427
BstDSI CCRYGG 1 cut(s) 129
BstF5I GGATG 1 cut(s) 119
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 1 cut(s) 244
BstMAI GTCTC 3 cut(s) 85, 243, 371
BstMBI GATC 1 cut(s) 241
BstMWI GCNNNNNNNGC 4 cut(s) 49, 134, 161, 446
BstNI CCWGG 3 cut(s) 29, 360, 465
BstSCI CCNGG 5 cut(s) 27, 270, 271, 358, 463
BstSLI GKGCMC 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 36
BsuRI GGCC 4 cut(s) 27, 43, 270, 449
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 1 cut(s) 119
BtsIMutI CAGTG 1 cut(s) 435
Cac8I GCNNGC 1 cut(s) 41
CfoI GCGC 1 cut(s) 137
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 4 cut(s) 25, 42, 269, 448
Cfr9I CCCGGG 1 cut(s) 271
CseI GACGC 2 cut(s) 91, 335
CsiI ACCWGGT 1 cut(s) 358
CviAII CATG 7 cut(s) 46, 159, 245, 290, 392, 445, 485
DdeI CTNAG 1 cut(s) 427
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
DraIII CACNNNGTG 1 cut(s) 362
EciI GGCGGA 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 271
EcoRI GAATTC 2 cut(s) 12, 256
EcoRII CCWGG 3 cut(s) 27, 358, 463
EcoT14I CCWWGG 1 cut(s) 338
ErhI CCWWGG 1 cut(s) 338
Esp3I CGTCTC 1 cut(s) 243
FaeI CATG 7 cut(s) 49, 162, 248, 293, 395, 448, 488
FaqI GGGAC 3 cut(s) 178, 426, 503
FatI CATG 7 cut(s) 45, 158, 244, 289, 391, 444, 484
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 1 cut(s) 50
FokI GGATG 1 cut(s) 106
Fsp4HI GCNGC 1 cut(s) 50
FspBI CTAG 1 cut(s) 284
GlaI GCGC 1 cut(s) 136
GluI GCNGC 1 cut(s) 50
GsaI CCCAGC 1 cut(s) 455
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 4 cut(s) 27, 43, 270, 449
HapII CCGG 2 cut(s) 146, 272
HgaI GACGC 2 cut(s) 91, 335
HhaI GCGC 1 cut(s) 137
Hin1II CATG 7 cut(s) 49, 162, 248, 293, 395, 448, 488
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HinfI GANTC 3 cut(s) 279, 315, 504
HpaII CCGG 2 cut(s) 146, 272
Hpy188I TCNGA 2 cut(s) 124, 307
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 1 cut(s) 416
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 2 cut(s) 75, 391
HpyF10VI GCNNNNNNNGC 4 cut(s) 49, 134, 161, 446
HpyF3I CTNAG 1 cut(s) 427
HpySE526I ACGT 1 cut(s) 182
Hsp92II CATG 7 cut(s) 49, 162, 248, 293, 395, 448, 488
HspAI GCGC 1 cut(s) 135
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 1 cut(s) 241
LmnI GCTCC 1 cut(s) 437
Lsp1109I GCAGC 1 cut(s) 36
MabI ACCWGGT 1 cut(s) 358
MaeI CTAG 1 cut(s) 284
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 2 cut(s) 200, 469
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MboII GAAGA 2 cut(s) 232, 324
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 3 cut(s) 12, 214, 256
MlyI GAGTC 1 cut(s) 288
MnlI CCTC 3 cut(s) 57, 269, 427
MspA1I CMGCKG 1 cut(s) 39
MspI CCGG 2 cut(s) 146, 272
MspR9I CCNGG 5 cut(s) 29, 272, 273, 360, 465
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 3 cut(s) 29, 360, 465
MwoI GCNNNNNNNGC 4 cut(s) 49, 134, 161, 446
NciI CCSGG 2 cut(s) 272, 273
NdeII GATC 1 cut(s) 241
NlaIII CATG 7 cut(s) 49, 162, 248, 293, 395, 448, 488
NlaIV GGNNCC 2 cut(s) 404, 450
NmuCI GTSAC 1 cut(s) 469
PctI GAATGC 1 cut(s) 214
PfeI GAWTC 2 cut(s) 315, 504
PflFI GACNNNGTC 1 cut(s) 96
PkrI GCNGC 1 cut(s) 51
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
Psp6I CCWGG 3 cut(s) 27, 358, 463
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 3 cut(s) 27, 358, 463
PspN4I GGNNCC 2 cut(s) 404, 450
PspPI GGNCC 4 cut(s) 25, 42, 269, 448
PsyI GACNNNGTC 1 cut(s) 96
PvuII CAGCTG 1 cut(s) 39
SatI GCNGC 1 cut(s) 50
Sau3AI GATC 1 cut(s) 241
Sau96I GGNCC 4 cut(s) 25, 42, 269, 448
SchI GAGTC 1 cut(s) 288
ScrFI CCNGG 5 cut(s) 29, 272, 273, 360, 465
SduI GDGCHC 1 cut(s) 405
SetI ASST 7 cut(s) 41, 185, 364, 408, 428, 442, 457
SexAI ACCWGGT 1 cut(s) 358
SmaI CCCGGG 1 cut(s) 273
Sse9I AATT 3 cut(s) 12, 214, 256
SsiI CCGC 1 cut(s) 32
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 5 cut(s) 27, 270, 271, 358, 463
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 1 cut(s) 382
TaiI ACGT 1 cut(s) 185
TasI AATT 3 cut(s) 12, 214, 256
TfiI GAWTC 2 cut(s) 315, 504
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 1 cut(s) 469
TseI GCWGC 1 cut(s) 49
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 4 cut(s) 5, 249, 408, 473
TspGWI ACGGA 1 cut(s) 76
TspMI CCCGGG 1 cut(s) 271
TspRI CASTG 1 cut(s) 435
Tth111I GACNNNGTC 1 cut(s) 96
XapI RAATTY 3 cut(s) 12, 214, 256
XmaI CCCGGG 1 cut(s) 271
XspI CTAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.