Rroxscaffold_5G00345060

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000309
Physical Location & Seq
Forward (+)
15318480 .. 15321740
3261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00345060.1

Sequence Viewer

Length: 516 bp
ATGATGATGCCCCATTGCGGTGTGCCGGATCATATTGGCGTCAAAAAGCAAAAATGGCCTCCTGCCAAGTACCACCTCACCTACGCTTTCGCTCCGGGCACTCTATCCGAAGCCAAGACTCCAGTTGCACAAGCTTTTCAAACTTGGGCGGCCAGCACTCACTTCAAGTTCAGACGACACAAAGATTACAAGAAGGCTGACATGAAGATCAGTTTCGAACAAGGGGATCACGGAGATGGGAGCTCCTTTGACGGCGCCGGTGGAATTCTAGCTCATGCTTATGCACCGACTTATGGAAAGCTCCACTACGACGCTGACGAGCCCTGGTCCATCGGCGCCTTGGAAGGTTATTATGACTTGGAGACAGTGGCTTTGCATGAGATTGGTCACCTTCTCGGACTTGCTCATAGCTCGGTTGAGGGAGCTATCATGTACCCTTACATCTCTTCTGGAGTTACCAAGCTGGATTTGCATCATGACGATATTCACGGAATTAGAGCTTTATACAAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

171

Amino Acids

18.91

Weight (kDa)

6.52

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 19 - 169 1.2e-47 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 254, 335
AciI CCGC 2 cut(s) 18, 149
AclWI GGATC 2 cut(s) 36, 234
AcoI YGGCCR 1 cut(s) 150
AcsI RAATTY 1 cut(s) 264
AcyI GRCGYC 3 cut(s) 39, 255, 336
AfaI GTAC 2 cut(s) 71, 434
AfiI CCNNNNNNNGG 2 cut(s) 17, 293
AgsI TTSAA 2 cut(s) 140, 166
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 8 cut(s) 134, 243, 272, 301, 411, 425, 463, 500
AluI AGCT 8 cut(s) 134, 243, 272, 301, 411, 425, 463, 500
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 2 cut(s) 36, 234
AoxI GGCC 2 cut(s) 56, 150
ApoI RAATTY 1 cut(s) 264
ArsI GACNNNNNNTTYG 2 cut(s) 355, 387
AspLEI GCGC 2 cut(s) 257, 338
AspS9I GGNCC 1 cut(s) 327
AsuC2I CCSGG 1 cut(s) 96
AsuHPI GGTGA 2 cut(s) 70, 380
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 1 cut(s) 327
BaeGI GKGCMC 1 cut(s) 101
BanI GGYRCC 2 cut(s) 254, 335
BanII GRGCYC 2 cut(s) 245, 324
Bbv12I GWGCWC 1 cut(s) 245
BccI CCATC 2 cut(s) 230, 338
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 1 cut(s) 325
BcnI CCSGG 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 1 cut(s) 269
BfoI RGCGCY 2 cut(s) 258, 339
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
Bme1390I CCNGG 2 cut(s) 96, 325
Bme18I GGWCC 1 cut(s) 327
BmgT120I GGNCC 1 cut(s) 327
BmiI GGNNCC 2 cut(s) 256, 337
BmrFI CCNGG 2 cut(s) 96, 325
BmsI GCATC 1 cut(s) 481
BpmI CTGGAG 2 cut(s) 105, 471
Bpu14I TTCGAA 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 96
BsaBI GATNNNNATC 1 cut(s) 471
BsaHI GRCGYC 3 cut(s) 39, 255, 336
BsaJI CCNNGG 2 cut(s) 323, 339
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 293
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 122
Bse3DI GCAATG 1 cut(s) 13
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 2 cut(s) 323, 339
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 2 cut(s) 17, 293
BseMI GCAATG 1 cut(s) 13
BseNI ACTGG 1 cut(s) 122
BseSI GKGCMC 1 cut(s) 101
BshFI GGCC 2 cut(s) 58, 152
BshNI GGYRCC 2 cut(s) 254, 335
BsiHKAI GWGCWC 1 cut(s) 245
BsiSI CCGG 3 cut(s) 26, 95, 258
BslI CCNNNNNNNGG 2 cut(s) 17, 293
BsmAI GTCTC 1 cut(s) 356
BsnI GGCC 2 cut(s) 58, 152
Bsp119I TTCGAA 1 cut(s) 216
Bsp1286I GDGCHC 3 cut(s) 101, 245, 324
Bsp143I GATC 3 cut(s) 28, 207, 226
BspACI CCGC 2 cut(s) 18, 149
BspANI GGCC 2 cut(s) 58, 152
BspHI TCATGA 1 cut(s) 475
BspLI GGNNCC 2 cut(s) 256, 337
BspPI GGATC 2 cut(s) 36, 234
BspT104I TTCGAA 1 cut(s) 216
BspT107I GGYRCC 2 cut(s) 254, 335
BsrDI GCAATG 1 cut(s) 13
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 323, 339
BssMI GATC 3 cut(s) 28, 207, 226
BssNI GRCGYC 3 cut(s) 39, 255, 336
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 367
Bst6I CTCTTC 1 cut(s) 451
BstACI GRCGYC 3 cut(s) 39, 255, 336
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 154
BstEII GGTNACC 1 cut(s) 386
BstH2I RGCGCY 2 cut(s) 258, 339
BstHHI GCGC 2 cut(s) 257, 338
BstKTI GATC 3 cut(s) 31, 210, 229
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 3 cut(s) 28, 207, 226
BstMWI GCNNNNNNNGC 2 cut(s) 55, 469
BstNI CCWGG 1 cut(s) 325
BstPI GGTNACC 1 cut(s) 386
BstSCI CCNGG 2 cut(s) 94, 323
BstSLI GKGCMC 1 cut(s) 101
BsuRI GGCC 2 cut(s) 58, 152
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 1 cut(s) 154
CciI TCATGA 1 cut(s) 475
CfoI GCGC 2 cut(s) 257, 338
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 1 cut(s) 327
CseI GACGC 2 cut(s) 28, 320
Csp6I GTAC 2 cut(s) 70, 433
CviAII CATG 5 cut(s) 202, 275, 377, 430, 476
CviQI GTAC 2 cut(s) 70, 433
DinI GGCGCC 2 cut(s) 256, 337
DpnI GATC 3 cut(s) 30, 209, 228
DpnII GATC 3 cut(s) 28, 207, 226
EaeI YGGCCR 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
Ecl136II GAGCTC 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 339
Eco24I GRGCYC 2 cut(s) 245, 324
Eco47I GGWCC 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 243
Eco91I GGTNACC 1 cut(s) 386
EcoICRI GAGCTC 1 cut(s) 243
EcoO65I GGTNACC 1 cut(s) 386
EcoRI GAATTC 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 339
EcoT38I GRGCYC 2 cut(s) 245, 324
EgeI GGCGCC 2 cut(s) 256, 337
EheI GGCGCC 2 cut(s) 256, 337
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 5 cut(s) 205, 278, 380, 433, 479
FatI CATG 5 cut(s) 201, 274, 376, 429, 475
Fnu4HI GCNGC 1 cut(s) 150
FriOI GRGCYC 2 cut(s) 245, 324
Fsp4HI GCNGC 1 cut(s) 150
FspBI CTAG 1 cut(s) 269
GlaI GCGC 2 cut(s) 256, 337
GluI GCNGC 1 cut(s) 150
GsuI CTGGAG 2 cut(s) 105, 471
HaeII RGCGCY 2 cut(s) 258, 339
HaeIII GGCC 2 cut(s) 58, 152
HapII CCGG 3 cut(s) 26, 95, 258
HgaI GACGC 2 cut(s) 28, 320
HhaI GCGC 2 cut(s) 257, 338
Hin1I GRCGYC 3 cut(s) 39, 255, 336
Hin1II CATG 5 cut(s) 205, 278, 380, 433, 479
Hin6I GCGC 2 cut(s) 255, 336
HinP1I GCGC 2 cut(s) 255, 336
HindIII AAGCTT 1 cut(s) 132
HinfI GANTC 1 cut(s) 118
HpaII CCGG 3 cut(s) 26, 95, 258
HphI GGTGA 2 cut(s) 70, 380
Hpy188I TCNGA 3 cut(s) 109, 173, 398
Hpy188III TCNNGA 3 cut(s) 450, 476, 513
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 3 cut(s) 187, 338, 401
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4V TGCA 4 cut(s) 128, 284, 376, 472
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 469
Hsp92I GRCGYC 3 cut(s) 39, 255, 336
Hsp92II CATG 5 cut(s) 205, 278, 380, 433, 479
HspAI GCGC 2 cut(s) 255, 336
KasI GGCGCC 2 cut(s) 254, 335
Kzo9I GATC 3 cut(s) 28, 207, 226
LmnI GCTCC 5 cut(s) 97, 240, 248, 306, 422
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 269
MaeIII GTNAC 2 cut(s) 386, 454
MalI GATC 3 cut(s) 30, 209, 228
MboI GATC 3 cut(s) 28, 207, 226
MboII GAAGA 2 cut(s) 217, 438
MhlI GDGCHC 3 cut(s) 101, 245, 324
MluCI AATT 2 cut(s) 264, 492
Mly113I GGCGCC 2 cut(s) 255, 336
MlyI GAGTC 1 cut(s) 112
MnlI CCTC 3 cut(s) 69, 86, 412
MslI CAYNNNNRTG 3 cut(s) 18, 234, 279
MspI CCGG 3 cut(s) 26, 95, 258
MspR9I CCNGG 2 cut(s) 96, 325
MvaI CCWGG 1 cut(s) 325
MwoI GCNNNNNNNGC 2 cut(s) 55, 469
NarI GGCGCC 2 cut(s) 255, 336
NciI CCSGG 1 cut(s) 96
NdeII GATC 3 cut(s) 28, 207, 226
NlaIII CATG 5 cut(s) 205, 278, 380, 433, 479
NlaIV GGNNCC 2 cut(s) 256, 337
NmuCI GTSAC 1 cut(s) 386
NspV TTCGAA 1 cut(s) 216
PagI TCATGA 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PkrI GCNGC 1 cut(s) 151
PleI GAGTC 1 cut(s) 112
PluTI GGCGCC 2 cut(s) 258, 339
PpsI GAGTC 1 cut(s) 112
Psp124BI GAGCTC 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 323
PspEI GGTNACC 1 cut(s) 386
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 2 cut(s) 256, 337
PspPI GGNCC 1 cut(s) 327
RsaI GTAC 2 cut(s) 71, 434
RsaNI GTAC 2 cut(s) 70, 433
RseI CAYNNNNRTG 3 cut(s) 18, 234, 279
SacI GAGCTC 1 cut(s) 245
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 3 cut(s) 28, 207, 226
Sau96I GGNCC 1 cut(s) 327
SchI GAGTC 1 cut(s) 112
ScrFI CCNGG 2 cut(s) 96, 325
SduI GDGCHC 3 cut(s) 101, 245, 324
SfaNI GCATC 1 cut(s) 481
SfoI GGCGCC 2 cut(s) 256, 337
SfuI TTCGAA 1 cut(s) 216
SgrAI CRCCGGYG 1 cut(s) 257
SinI GGWCC 1 cut(s) 327
SmiMI CAYNNNNRTG 3 cut(s) 18, 234, 279
Sse9I AATT 2 cut(s) 264, 492
SsiI CCGC 2 cut(s) 18, 149
SspDI GGCGCC 2 cut(s) 254, 335
SspMI CTAG 1 cut(s) 269
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 2 cut(s) 94, 323
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 1 cut(s) 367
TaqI TCGA 1 cut(s) 216
TasI AATT 2 cut(s) 264, 492
TauI GCSGC 1 cut(s) 152
TscAI CASTG 1 cut(s) 372
TseFI GTSAC 1 cut(s) 386
Tsp45I GTSAC 1 cut(s) 386
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 2 cut(s) 246, 504
TspRI CASTG 1 cut(s) 372
VpaK11BI GGWCC 1 cut(s) 327
XapI RAATTY 1 cut(s) 264
XcmI CCANNNNNNNNNTGG 1 cut(s) 337
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.