RLG00000021929

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000309
Physical Location & Seq
Reverse (-)
79360883 .. 79362017
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021929

Sequence Viewer

Length: 918 bp
ATGGCACCAAAATCTGATCTTTCTCTTTTCACAGTCACTCTCCTCCTCCTCCTCGCCATCTTTTCTTTCCTTTGTAATGCAACGGCAAACTCATCTCCGTTTGAGTTCCTCAAGCATCTTAAGGGTTGTCACAAAGGTGACAAGGTCAAGGGCATCAATGACCTCAAGAAGTACCTTCACAACTTCGGTTACTTGAACTACAAGAACCACATTCATTCTGATGACGATGATTTCGACGAGCTCTTGGAGGAAGCCGTCAAGACTTACCAGCTCAATTTCCACCTCAAGTCCACCGGAACGTTGGACGACAAAACCATATTACAGATGATGATGCCTCGTTGTGGTGTGCCCGATATCGTCAATGGCACCACATCCATGCGATTAGCCAAGATAAAGCACCAGCACTGTTCAATTCATACCACTGTTCACTACTCATTTCCTAATGGAAAACCAAAATGGCCTTCCTCTAAATACCATCTGACATACGCTTTCTTCCCCGGCACCCCATCCCAAGCCACGGGTGCTATAGCACAGGCTTTCGCAACATGGGCTAGAAGCACTCACTTCAAGTTCAGTCAGGCCCAAAATTACCAGAATGCAGATGTCAAGATTAGTTTTCACCGAGGTAATCATGGAGATGGGCGTAATTTTGATGGGCCTGGCGGAGTGCTGGCCCATGCTTTTTACCCTATGGATGGAAGATTCCACTTCGACGCAGCTGAGAATTGGTCTGTGGGTGCTAAACCAGGTGCCTATGACTTGGAAAGCGTTGCTTTGCATGAAATAGGACACCTTCTGGGACTTGGACATAGCTCAGTGAAGGGAGCTATCATGTCAGCAACCATCTCCCCGGGAGTGACTCTTAAAAGTTTGCATGGGGACGATATTCAAGGAATCAAAACTTTATACAACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

306

Amino Acids

33.72

Weight (kDa)

8.52

Isoelectric Point (pI)

22.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 52 - 107 5e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 152 - 303 2.5e-47 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 4, 365, 500, 749
AciI CCGC 1 cut(s) 663
AclI AACGTT 1 cut(s) 299
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 3 cut(s) 341, 517, 695
AflII CTTAAG 1 cut(s) 119
AgsI TTSAA 4 cut(s) 196, 411, 568, 890
AjnI CCWGG 2 cut(s) 658, 745
AjuI GAANNNNNNNTTGG 2 cut(s) 445, 477
AleI CACNNNNGTG 1 cut(s) 135
AluBI AGCT 5 cut(s) 241, 271, 719, 813, 827
AluI AGCT 5 cut(s) 241, 271, 719, 813, 827
Alw21I GWGCWC 1 cut(s) 243
Ama87I CYCGRG 1 cut(s) 850
AoxI GGCC 4 cut(s) 458, 579, 656, 672
ApeKI GCWGC 1 cut(s) 716
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
AspS9I GGNCC 3 cut(s) 580, 656, 673
AsuC2I CCSGG 3 cut(s) 498, 851, 852
AsuHPI GGTGA 2 cut(s) 149, 611
AvaI CYCGRG 1 cut(s) 850
BaeGI GKGCMC 1 cut(s) 351
BanI GGYRCC 4 cut(s) 4, 365, 500, 749
BanII GRGCYC 1 cut(s) 243
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 1 cut(s) 728
BccI CCATC 7 cut(s) 65, 483, 514, 632, 647, 689, 851
BceAI ACGGC 2 cut(s) 99, 239
BciT130I CCWGG 2 cut(s) 660, 747
BcnI CCSGG 3 cut(s) 498, 851, 852
BfaI CTAG 1 cut(s) 552
BfmI CTRYAG 1 cut(s) 525
BfrI CTTAAG 1 cut(s) 119
BisI GCNGC 1 cut(s) 717
BlsI GCNGC 1 cut(s) 718
Bme1390I CCNGG 5 cut(s) 498, 660, 747, 851, 852
BmeT110I CYCGRG 1 cut(s) 850
BmgT120I GGNCC 3 cut(s) 580, 656, 673
BmiI GGNNCC 4 cut(s) 6, 367, 502, 751
BmrFI CCNGG 5 cut(s) 498, 660, 747, 851, 852
BmsI GCATC 3 cut(s) 124, 162, 321
BpuEI CTTGAG 3 cut(s) 95, 149, 269
BpuMI CCSGG 3 cut(s) 498, 851, 852
BsaJI CCNNGG 5 cut(s) 496, 516, 622, 849, 850
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 3 cut(s) 341, 517, 695
BseBI CCWGG 2 cut(s) 660, 747
BseDI CCNNGG 5 cut(s) 496, 516, 622, 849, 850
BseGI GGATG 3 cut(s) 371, 506, 700
BseLI CCNNNNNNNGG 3 cut(s) 341, 517, 695
BseMII CTCAG 2 cut(s) 711, 828
BseRI GAGGAG 4 cut(s) 32, 35, 38, 41
BseSI GKGCMC 1 cut(s) 351
BseXI GCAGC 1 cut(s) 728
BshFI GGCC 4 cut(s) 460, 581, 658, 674
BshNI GGYRCC 4 cut(s) 4, 365, 500, 749
BsiHKAI GWGCWC 1 cut(s) 243
BsiHKCI CYCGRG 1 cut(s) 850
BsiSI CCGG 3 cut(s) 294, 498, 851
BslFI GGGAC 2 cut(s) 813, 893
BslI CCNNNNNNNGG 3 cut(s) 341, 517, 695
BsmFI GGGAC 2 cut(s) 813, 893
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 4 cut(s) 460, 581, 658, 674
BsoBI CYCGRG 1 cut(s) 850
Bsp1286I GDGCHC 2 cut(s) 243, 351
Bsp143I GATC 1 cut(s) 16
BspACI CCGC 1 cut(s) 663
BspANI GGCC 4 cut(s) 460, 581, 658, 674
BspCNI CTCAG 2 cut(s) 712, 827
BspLI GGNNCC 4 cut(s) 6, 367, 502, 751
BspT107I GGYRCC 4 cut(s) 4, 365, 500, 749
BspTI CTTAAG 1 cut(s) 119
BssECI CCNNGG 5 cut(s) 496, 516, 622, 849, 850
BssMI GATC 1 cut(s) 16
Bst2UI CCWGG 2 cut(s) 660, 747
Bst4CI ACNGT 3 cut(s) 34, 407, 424
BstAFI CTTAAG 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 672
BstDEI CTNAG 2 cut(s) 720, 814
BstDSI CCRYGG 1 cut(s) 516
BstF5I GGATG 3 cut(s) 371, 506, 700
BstKTI GATC 1 cut(s) 19
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 2 cut(s) 521, 548
BstNI CCWGG 2 cut(s) 660, 747
BstSCI CCNGG 5 cut(s) 496, 658, 745, 849, 850
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 728
BsuRI GGCC 4 cut(s) 460, 581, 658, 674
BtgI CCRYGG 1 cut(s) 516
BtsCI GGATG 3 cut(s) 371, 506, 700
BtsIMutI CAGTG 3 cut(s) 403, 420, 822
Cac8I GCNNGC 1 cut(s) 672
Cfr13I GGNCC 3 cut(s) 580, 656, 673
Cfr9I CCCGGG 1 cut(s) 850
CseI GACGC 1 cut(s) 722
CsiI ACCWGGT 1 cut(s) 745
Csp6I GTAC 1 cut(s) 172
CviAII CATG 7 cut(s) 376, 546, 632, 677, 779, 832, 875
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 2 cut(s) 720, 814
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
EciI GGCGGA 1 cut(s) 678
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 1 cut(s) 243
Eco32I GATATC 1 cut(s) 355
Eco53kI GAGCTC 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 850
EcoICRI GAGCTC 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 658, 745
EcoRV GATATC 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 243
FaeI CATG 7 cut(s) 379, 549, 635, 680, 782, 835, 878
FalI AAGNNNNNCTT 2 cut(s) 757, 789
FaqI GGGAC 2 cut(s) 813, 893
FatI CATG 7 cut(s) 375, 545, 631, 676, 778, 831, 874
Fnu4HI GCNGC 1 cut(s) 717
FokI GGATG 3 cut(s) 358, 493, 707
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 1 cut(s) 717
FspBI CTAG 1 cut(s) 552
GluI GCNGC 1 cut(s) 717
HaeIII GGCC 4 cut(s) 460, 581, 658, 674
HapII CCGG 3 cut(s) 294, 498, 851
HgaI GACGC 1 cut(s) 722
Hin1II CATG 7 cut(s) 379, 549, 635, 680, 782, 835, 878
HinfI GANTC 3 cut(s) 702, 859, 894
HpaII CCGG 3 cut(s) 294, 498, 851
HphI GGTGA 2 cut(s) 149, 611
Hpy166II GTNNAC 2 cut(s) 291, 427
Hpy188I TCNGA 4 cut(s) 16, 220, 480, 917
Hpy188III TCNNGA 3 cut(s) 166, 259, 607
Hpy8I GTNNAC 2 cut(s) 291, 427
Hpy99I CGWCG 2 cut(s) 239, 716
HpyAV CCTTC 4 cut(s) 185, 471, 803, 814
HpyCH4III ACNGT 3 cut(s) 34, 407, 424
HpyCH4IV ACGT 2 cut(s) 299, 912
HpyCH4V TGCA 4 cut(s) 80, 599, 778, 874
HpyF10VI GCNNNNNNNGC 2 cut(s) 521, 548
HpyF3I CTNAG 2 cut(s) 720, 814
HpySE526I ACGT 2 cut(s) 299, 912
Hsp92II CATG 7 cut(s) 379, 549, 635, 680, 782, 835, 878
Kzo9I GATC 1 cut(s) 16
LmnI GCTCC 1 cut(s) 824
Lsp1109I GCAGC 1 cut(s) 728
LweI GCATC 3 cut(s) 124, 162, 321
MabI ACCWGGT 1 cut(s) 745
MaeI CTAG 1 cut(s) 552
MaeII ACGT 2 cut(s) 299, 912
MaeIII GTNAC 5 cut(s) 34, 128, 137, 188, 856
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 2 cut(s) 484, 711
MhlI GDGCHC 2 cut(s) 243, 351
MluCI AATT 5 cut(s) 274, 411, 586, 646, 724
MlyI GAGTC 1 cut(s) 853
MmeI TCCRAC 1 cut(s) 282
MseI TTAA 2 cut(s) 120, 864
MslI CAYNNNNRTG 4 cut(s) 135, 219, 374, 636
MspA1I CMGCKG 1 cut(s) 719
MspCI CTTAAG 1 cut(s) 119
MspI CCGG 3 cut(s) 294, 498, 851
MspR9I CCNGG 5 cut(s) 498, 660, 747, 851, 852
Mva1269I GAATGC 1 cut(s) 601
MvaI CCWGG 2 cut(s) 660, 747
MwoI GCNNNNNNNGC 2 cut(s) 521, 548
NciI CCSGG 3 cut(s) 498, 851, 852
NdeII GATC 1 cut(s) 16
NlaIII CATG 7 cut(s) 379, 549, 635, 680, 782, 835, 878
NlaIV GGNNCC 4 cut(s) 6, 367, 502, 751
NmuCI GTSAC 4 cut(s) 34, 128, 137, 856
OliI CACNNNNGTG 1 cut(s) 135
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 601
PfeI GAWTC 2 cut(s) 702, 894
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 1 cut(s) 718
PleI GAGTC 1 cut(s) 853
PpsI GAGTC 1 cut(s) 853
Psp124BI GAGCTC 1 cut(s) 243
Psp1406I AACGTT 1 cut(s) 299
Psp6I CCWGG 2 cut(s) 658, 745
PspGI CCWGG 2 cut(s) 658, 745
PspN4I GGNNCC 4 cut(s) 6, 367, 502, 751
PspPI GGNCC 3 cut(s) 580, 656, 673
PsyI GACNNNGTC 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 4 cut(s) 135, 219, 374, 636
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 120, 864
SatI GCNGC 1 cut(s) 717
Sau3AI GATC 1 cut(s) 16
Sau96I GGNCC 3 cut(s) 580, 656, 673
SchI GAGTC 1 cut(s) 853
ScrFI CCNGG 5 cut(s) 498, 660, 747, 851, 852
SduI GDGCHC 2 cut(s) 243, 351
SexAI ACCWGGT 1 cut(s) 745
SfaNI GCATC 3 cut(s) 124, 162, 321
SfcI CTRYAG 1 cut(s) 525
SmaI CCCGGG 1 cut(s) 852
SmiMI CAYNNNNRTG 4 cut(s) 135, 219, 374, 636
SmlI CTYRAG 4 cut(s) 110, 119, 164, 284
SmoI CTYRAG 4 cut(s) 110, 119, 164, 284
Sse9I AATT 5 cut(s) 274, 411, 586, 646, 724
SsiI CCGC 1 cut(s) 663
SspMI CTAG 1 cut(s) 552
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 5 cut(s) 496, 658, 745, 849, 850
TaaI ACNGT 3 cut(s) 34, 407, 424
TaiI ACGT 2 cut(s) 302, 915
TaqI TCGA 2 cut(s) 234, 711
TasI AATT 5 cut(s) 274, 411, 586, 646, 724
TfiI GAWTC 2 cut(s) 702, 894
Tru1I TTAA 2 cut(s) 120, 864
Tru9I TTAA 2 cut(s) 120, 864
TscAI CASTG 3 cut(s) 410, 427, 822
TseFI GTSAC 4 cut(s) 34, 128, 137, 856
TseI GCWGC 1 cut(s) 716
Tsp45I GTSAC 4 cut(s) 34, 128, 137, 856
TspDTI ATGAA 3 cut(s) 203, 404, 795
TspGWI ACGGA 1 cut(s) 87
TspMI CCCGGG 1 cut(s) 850
TspRI CASTG 3 cut(s) 410, 427, 822
Tth111I GACNNNGTC 1 cut(s) 143
Vha464I CTTAAG 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XmaI CCCGGG 1 cut(s) 850
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.