Rorug02G0546300

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000309
Physical Location & Seq
Reverse (-)
67512300 .. 67514007
1708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0546300.1

Sequence Viewer

Length: 1284 bp
ATGATTTTGGTTCCGTCAAATTTCCAACGCTTGCTTTCTTTCACCAAGCAATTGACTTCCCACGTCAACCAAGGCAGCCATGACAAAGCCCTCACCCTCTTCCACCACATGCAAGCCTCACTCGCTCTCCCTCTCGACGCCCACGTCTTCTCTCTCGTCCTCAAGTCCTGCTCCGCCGTCCGCCGCCCCCAACTCGGCGCCGCCGTGCACGCCCACGTCACCAAATCGTCGCTCCTCTCAAACCCTTTTGTGGCCTGCGCTCTCGTTGACATGTACGGCAAATGCGTCTCGCTTTGGGCTGCACGCCACTTGTTCGATGAAATTCCTCACAGAAATGTTGTTGTGTGGAATGCCATGATCTCGGTTTGTACCCGCAATGCTGACGTTGCTGAAGCTTTGAGGTTGTTTGAGGCTATGGATGTTGACCCTGATGTGTCTACTTATAACACCATTATAGCCGGAACGTCGGGGGTGGATGATGGGTTGTTTAGGGCTGTGGAGGTGTATAGGAAAATGTGCCAAAGAGGGTGGAAGCCGGATTTGATCACACTTCTTGCTCTGTTACGTGCTTGTGTTGGTGTAGCAGCTTTTAGGTTTATTAAGGAAATTCATGGTTATGATGTAAGGAATGGGATTGACTCGCATTCGCAGTTGAGTAGCGGTTTAGTAGAGGCCTATGGGCGCTGTGGTTGTGTCGAAAATGCGCATAGTGTTTTCCGGGGTTTGAAGGAGAAGGATGTGGTTGCGTGGAGCAGTTTGATATCAGTGTATGCTGTTCATGGGGAGGCAAGAGCTGCATTTGATGTTTTTAGAGAAATGGAATCGGCGAAAGTGGTGCCTGATGAGATTACTTTTCTTGCGGTACTGAAAGCGTGTAGCCATGCTGAAGGATTAGCTGATGAAGCACTGAGTTATTTTGATTGTATGCGTAATTACGGCGTCCAAGCAAACAGTGATCACTATTCTTGTTTAGTGGATGTTTTGAGCAGAGCAGGGAGGTTATGCGAGGCGTATGAGGTCATAAGGAAGATGCCAGTGAAGGTGACTGCTAGAGCTTGGGGTGCCCTTCTTTCGGGGTGTAGAACTTATGGAAACCTGGAGCTCGCGGAGATAGCAGGGAAAGCTTTGTCCGAACTTGAGCCCGATAATCCTTCCCATTATTTATTGTTGTCAACGATTTATGTTAGCTTAGGAAGGCATGATGAAGCTCAGAGAATGAGAAGGGAAATGAGGCAGAAAGGTGTGAAGGCACCGGTTGGAAGTAGTTGGATGGTTCAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

427

Amino Acids

47.19

Weight (kDa)

7.25

Isoelectric Point (pI)

31.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 111 - 154 2.3e-09 PPR repeat family
PPR_2 PF13041 143 - 192 4.4e-07 PPR repeat family
PPR_2 PF13041 245 - 293 1.2e-10 PPR repeat family
PPR_3 PF13812 245 - 292 1.1e-06 Pentatricopeptide repeat domain
PPR PF01535 248 - 276 7.4e-06 PPR repeat
PPR_long PF17177 249 - 348 2.3e-07 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 363 - 424 8.2e-14 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 444
AasI GACNNNNNNGTC 1 cut(s) 143
Acc16I TGCGCA 1 cut(s) 705
AccB1I GGYRCC 4 cut(s) 197, 835, 1061, 1249
AccI GTMKAC 1 cut(s) 437
AccII CGCG 1 cut(s) 1106
AciI CCGC 8 cut(s) 174, 181, 184, 201, 373, 660, 860, 1106
AcsI RAATTY 3 cut(s) 19, 321, 606
AcuI CTGAAG 2 cut(s) 411, 906
AcyI GRCGYC 3 cut(s) 138, 198, 939
AfaI GTAC 3 cut(s) 275, 370, 864
AfiI CCNNNNNNNGG 3 cut(s) 194, 250, 1072
AflIII ACRYGT 1 cut(s) 270
AgeI ACCGGT 1 cut(s) 1252
AgsI TTSAA 2 cut(s) 727, 1277
AjiI CACGTC 3 cut(s) 64, 145, 217
AjnI CCWGG 1 cut(s) 1095
AluBI AGCT 9 cut(s) 395, 587, 794, 896, 1055, 1102, 1124, 1188, 1208
AluI AGCT 9 cut(s) 395, 587, 794, 896, 1055, 1102, 1124, 1188, 1208
Alw21I GWGCWC 2 cut(s) 210, 1104
Alw26I GTCTC 1 cut(s) 292
Alw44I GTGCAC 1 cut(s) 206
AoxI GGCC 2 cut(s) 252, 672
ApaLI GTGCAC 1 cut(s) 206
ApeKI GCWGC 4 cut(s) 75, 299, 584, 794
ApoI RAATTY 3 cut(s) 19, 321, 606
AsiGI ACCGGT 1 cut(s) 1252
AspLEI GCGC 4 cut(s) 200, 260, 684, 706
AsuC2I CCSGG 1 cut(s) 719
AsuHPI GGTGA 4 cut(s) 34, 85, 211, 1054
BaeGI GKGCMC 2 cut(s) 210, 1066
BanI GGYRCC 4 cut(s) 197, 835, 1061, 1249
BanII GRGCYC 2 cut(s) 1104, 1143
BbsI GAAGAC 1 cut(s) 139
Bbv12I GWGCWC 2 cut(s) 210, 1104
BbvI GCAGC 4 cut(s) 87, 286, 596, 781
BccI CCATC 2 cut(s) 473, 1264
BceAI ACGGC 4 cut(s) 161, 188, 292, 952
BcgI CGANNNNNNTGC 2 cut(s) 817, 851
BciT130I CCWGG 1 cut(s) 1097
BclI TGATCA 2 cut(s) 543, 955
BcnI CCSGG 1 cut(s) 719
BcoDI GTCTC 1 cut(s) 292
BfaI CTAG 1 cut(s) 1050
BfoI RGCGCY 2 cut(s) 201, 685
BisI GCNGC 6 cut(s) 76, 184, 201, 300, 585, 795
BlsI GCNGC 6 cut(s) 77, 185, 202, 301, 586, 796
Bme1390I CCNGG 2 cut(s) 719, 1097
BmgBI CACGTC 3 cut(s) 64, 145, 217
BmiI GGNNCC 5 cut(s) 12, 199, 837, 1063, 1251
BmrFI CCNGG 2 cut(s) 719, 1097
BmsI GCATC 1 cut(s) 1020
BpiI GAAGAC 1 cut(s) 139
BpmI CTGGAG 1 cut(s) 1118
Bpu10I CCTNAGC 1 cut(s) 1189
BpuEI CTTGAG 2 cut(s) 146, 1157
BpuMI CCSGG 1 cut(s) 719
BsaAI YACGTR 1 cut(s) 566
BsaHI GRCGYC 3 cut(s) 138, 198, 939
BsaJI CCNNGG 2 cut(s) 70, 718
BsaWI WCCGGW 1 cut(s) 1252
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 250, 1072
Bse118I RCCGGY 1 cut(s) 1252
Bse1I ACTGG 1 cut(s) 1034
Bse3DI GCAATG 1 cut(s) 382
BseBI CCWGG 1 cut(s) 1097
BseDI CCNNGG 2 cut(s) 70, 718
BseGI GGATG 5 cut(s) 424, 481, 742, 982, 1275
BseLI CCNNNNNNNGG 3 cut(s) 194, 250, 1072
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 2 cut(s) 899, 1223
BseNI ACTGG 1 cut(s) 1034
BseRI GAGGAG 1 cut(s) 224
BseSI GKGCMC 2 cut(s) 210, 1066
BseXI GCAGC 4 cut(s) 87, 286, 596, 781
BsgI GTGCAG 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 1106
BshFI GGCC 2 cut(s) 254, 674
BshNI GGYRCC 4 cut(s) 197, 835, 1061, 1249
BshTI ACCGGT 1 cut(s) 1252
BsiHKAI GWGCWC 2 cut(s) 210, 1104
BsiSI CCGG 4 cut(s) 459, 536, 718, 1253
BslI CCNNNNNNNGG 3 cut(s) 194, 250, 1072
BsmAI GTCTC 1 cut(s) 292
BsmBI CGTCTC 1 cut(s) 292
BsmI GAATGC 2 cut(s) 355, 643
BsnI GGCC 2 cut(s) 254, 674
Bsp1286I GDGCHC 4 cut(s) 210, 1066, 1104, 1143
Bsp143I GATC 3 cut(s) 357, 543, 955
BspACI CCGC 8 cut(s) 174, 181, 184, 201, 373, 660, 860, 1106
BspANI GGCC 2 cut(s) 254, 674
BspCNI CTCAG 2 cut(s) 900, 1222
BspFNI CGCG 1 cut(s) 1106
BspLI GGNNCC 5 cut(s) 12, 199, 837, 1063, 1251
BspT107I GGYRCC 4 cut(s) 197, 835, 1061, 1249
BsrDI GCAATG 1 cut(s) 382
BsrFI RCCGGY 1 cut(s) 1252
BsrI ACTGG 1 cut(s) 1034
BssAI RCCGGY 1 cut(s) 1252
BssECI CCNNGG 2 cut(s) 70, 718
BssMI GATC 3 cut(s) 357, 543, 955
BssNI GRCGYC 3 cut(s) 138, 198, 939
BssT1I CCWWGG 1 cut(s) 70
Bst2UI CCWGG 1 cut(s) 1097
Bst4CI ACNGT 1 cut(s) 953
Bst6I CTCTTC 1 cut(s) 104
BstACI GRCGYC 3 cut(s) 138, 198, 939
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBAI YACGTR 1 cut(s) 566
BstC8I GCNNGC 6 cut(s) 32, 114, 210, 256, 304, 1104
BstDEI CTNAG 3 cut(s) 908, 1189, 1209
BstF5I GGATG 5 cut(s) 424, 481, 742, 982, 1275
BstFNI CGCG 1 cut(s) 1106
BstH2I RGCGCY 2 cut(s) 201, 685
BstHHI GCGC 4 cut(s) 200, 260, 684, 706
BstKTI GATC 3 cut(s) 360, 546, 958
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 3 cut(s) 357, 543, 955
BstMWI GCNNNNNNNGC 8 cut(s) 122, 209, 386, 794, 902, 1061, 1112, 1121
BstNI CCWGG 1 cut(s) 1097
BstNSI RCATGY 2 cut(s) 112, 274
BstSCI CCNGG 2 cut(s) 717, 1095
BstSLI GKGCMC 2 cut(s) 210, 1066
BstUI CGCG 1 cut(s) 1106
BstV1I GCAGC 4 cut(s) 87, 286, 596, 781
BstV2I GAAGAC 1 cut(s) 139
BsuRI GGCC 2 cut(s) 254, 674
BtrI CACGTC 3 cut(s) 64, 145, 217
BtsCI GGATG 5 cut(s) 424, 481, 742, 982, 1275
BtsIMutI CAGTG 4 cut(s) 771, 905, 958, 1041
Cac8I GCNNGC 6 cut(s) 32, 114, 210, 256, 304, 1104
CfoI GCGC 4 cut(s) 200, 260, 684, 706
Cfr10I RCCGGY 1 cut(s) 1252
CseI GACGC 3 cut(s) 146, 274, 928
Csp6I GTAC 3 cut(s) 274, 369, 863
CspAI ACCGGT 1 cut(s) 1252
CspCI CAANNNNNGTGG 2 cut(s) 509, 544
CviAII CATG 8 cut(s) 80, 109, 271, 355, 611, 779, 881, 1199
CviQI GTAC 3 cut(s) 274, 369, 863
DdeI CTNAG 3 cut(s) 908, 1189, 1209
DinI GGCGCC 1 cut(s) 199
DpnI GATC 3 cut(s) 359, 545, 957
DpnII GATC 3 cut(s) 357, 543, 955
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 2 cut(s) 163, 170
Ecl136II GAGCTC 1 cut(s) 1102
Eco130I CCWWGG 1 cut(s) 70
Eco147I AGGCCT 1 cut(s) 674
Eco24I GRGCYC 2 cut(s) 1104, 1143
Eco32I GATATC 1 cut(s) 762
Eco53kI GAGCTC 1 cut(s) 1102
Eco57I CTGAAG 2 cut(s) 411, 906
EcoICRI GAGCTC 1 cut(s) 1102
EcoRII CCWGG 1 cut(s) 1095
EcoRV GATATC 1 cut(s) 762
EcoT14I CCWWGG 1 cut(s) 70
EcoT38I GRGCYC 2 cut(s) 1104, 1143
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 70
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 8 cut(s) 83, 112, 274, 358, 614, 782, 884, 1202
FatI CATG 8 cut(s) 79, 108, 270, 354, 610, 778, 880, 1198
FauI CCCGC 1 cut(s) 380
FbaI TGATCA 2 cut(s) 543, 955
FblI GTMKAC 1 cut(s) 437
Fnu4HI GCNGC 6 cut(s) 76, 184, 201, 300, 585, 795
FokI GGATG 4 cut(s) 431, 488, 749, 989
FriOI GRGCYC 2 cut(s) 1104, 1143
Fsp4HI GCNGC 6 cut(s) 76, 184, 201, 300, 585, 795
FspAI RTGCGCAY 1 cut(s) 705
FspBI CTAG 1 cut(s) 1050
FspI TGCGCA 1 cut(s) 705
GlaI GCGC 4 cut(s) 199, 259, 683, 705
GluI GCNGC 6 cut(s) 76, 184, 201, 300, 585, 795
GsuI CTGGAG 1 cut(s) 1118
HaeII RGCGCY 2 cut(s) 201, 685
HaeIII GGCC 2 cut(s) 254, 674
HapII CCGG 4 cut(s) 459, 536, 718, 1253
HgaI GACGC 3 cut(s) 146, 274, 928
HhaI GCGC 4 cut(s) 200, 260, 684, 706
Hin1I GRCGYC 3 cut(s) 138, 198, 939
Hin1II CATG 8 cut(s) 83, 112, 274, 358, 614, 782, 884, 1202
Hin6I GCGC 4 cut(s) 198, 258, 682, 704
HinP1I GCGC 4 cut(s) 198, 258, 682, 704
HincII GTYRAC 4 cut(s) 67, 268, 424, 1173
HindII GTYRAC 4 cut(s) 67, 268, 424, 1173
HindIII AAGCTT 2 cut(s) 393, 1122
HinfI GANTC 2 cut(s) 638, 821
HpaII CCGG 4 cut(s) 459, 536, 718, 1253
HphI GGTGA 4 cut(s) 34, 85, 211, 1054
Hpy166II GTNNAC 6 cut(s) 67, 208, 268, 424, 438, 1173
Hpy188I TCNGA 2 cut(s) 1132, 1212
Hpy188III TCNNGA 2 cut(s) 134, 1277
Hpy8I GTNNAC 6 cut(s) 67, 208, 268, 424, 438, 1173
Hpy99I CGWCG 3 cut(s) 140, 232, 469
HpyAV CCTTC 9 cut(s) 721, 727, 881, 1033, 1076, 1161, 1188, 1215, 1240
HpyCH4III ACNGT 1 cut(s) 953
HpyCH4IV ACGT 6 cut(s) 63, 144, 216, 384, 464, 565
HpyCH4V TGCA 4 cut(s) 112, 208, 302, 797
HpyF10VI GCNNNNNNNGC 8 cut(s) 122, 209, 386, 794, 902, 1061, 1112, 1121
HpyF3I CTNAG 3 cut(s) 908, 1189, 1209
HpySE526I ACGT 6 cut(s) 63, 144, 216, 384, 464, 565
Hsp92I GRCGYC 3 cut(s) 138, 198, 939
Hsp92II CATG 8 cut(s) 83, 112, 274, 358, 614, 782, 884, 1202
HspAI GCGC 4 cut(s) 198, 258, 682, 704
KasI GGCGCC 1 cut(s) 197
Ksp22I TGATCA 2 cut(s) 543, 955
Kzo9I GATC 3 cut(s) 357, 543, 955
LmnI GCTCC 4 cut(s) 176, 237, 750, 1099
Lsp1109I GCAGC 4 cut(s) 87, 286, 596, 781
LweI GCATC 1 cut(s) 1020
MaeI CTAG 1 cut(s) 1050
MaeII ACGT 6 cut(s) 63, 144, 216, 384, 464, 565
MaeIII GTNAC 3 cut(s) 217, 561, 1042
MalI GATC 3 cut(s) 359, 545, 957
MboI GATC 3 cut(s) 357, 543, 955
MboII GAAGA 3 cut(s) 91, 139, 1039
MfeI CAATTG 1 cut(s) 50
MhlI GDGCHC 4 cut(s) 210, 1066, 1104, 1143
MluCI AATT 5 cut(s) 19, 50, 321, 606, 931
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 1 cut(s) 632
MmeI TCCRAC 3 cut(s) 49, 1237, 1247
MseI TTAA 1 cut(s) 600
MslI CAYNNNNRTG 2 cut(s) 333, 615
MspI CCGG 4 cut(s) 459, 536, 718, 1253
MspR9I CCNGG 2 cut(s) 719, 1097
MunI CAATTG 1 cut(s) 50
Mva1269I GAATGC 2 cut(s) 355, 643
MvaI CCWGG 1 cut(s) 1097
MvnI CGCG 1 cut(s) 1106
MwoI GCNNNNNNNGC 8 cut(s) 122, 209, 386, 794, 902, 1061, 1112, 1121
NarI GGCGCC 1 cut(s) 198
NciI CCSGG 1 cut(s) 719
NdeII GATC 3 cut(s) 357, 543, 955
NlaIII CATG 8 cut(s) 83, 112, 274, 358, 614, 782, 884, 1202
NlaIV GGNNCC 5 cut(s) 12, 199, 837, 1063, 1251
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 2 cut(s) 217, 1042
NsbI TGCGCA 1 cut(s) 705
NspI RCATGY 2 cut(s) 112, 274
PceI AGGCCT 1 cut(s) 674
PciI ACATGT 1 cut(s) 270
PcsI WCGNNNNNNNCGW 3 cut(s) 141, 201, 282
PctI GAATGC 2 cut(s) 355, 643
PfeI GAWTC 1 cut(s) 821
PinAI ACCGGT 1 cut(s) 1252
PkrI GCNGC 6 cut(s) 77, 185, 202, 301, 586, 796
PleI GAGTC 1 cut(s) 632
PluTI GGCGCC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 632
Ppu21I YACGTR 1 cut(s) 566
PscI ACATGT 1 cut(s) 270
PsiI TTATAA 1 cut(s) 444
Psp124BI GAGCTC 1 cut(s) 1104
Psp6I CCWGG 1 cut(s) 1095
PspGI CCWGG 1 cut(s) 1095
PspN4I GGNNCC 5 cut(s) 12, 199, 837, 1063, 1251
RsaI GTAC 3 cut(s) 275, 370, 864
RsaNI GTAC 3 cut(s) 274, 369, 863
RseI CAYNNNNRTG 2 cut(s) 333, 615
SacI GAGCTC 1 cut(s) 1104
SaqAI TTAA 1 cut(s) 600
SatI GCNGC 6 cut(s) 76, 184, 201, 300, 585, 795
Sau3AI GATC 3 cut(s) 357, 543, 955
SchI GAGTC 1 cut(s) 632
ScrFI CCNGG 2 cut(s) 719, 1097
SduI GDGCHC 4 cut(s) 210, 1066, 1104, 1143
SfaNI GCATC 1 cut(s) 1020
SfoI GGCGCC 1 cut(s) 199
SmiMI CAYNNNNRTG 2 cut(s) 333, 615
SmlI CTYRAG 2 cut(s) 161, 1136
SmoI CTYRAG 2 cut(s) 161, 1136
Sse9I AATT 5 cut(s) 19, 50, 321, 606, 931
SseBI AGGCCT 1 cut(s) 674
SsiI CCGC 8 cut(s) 174, 181, 184, 201, 373, 660, 860, 1106
SspDI GGCGCC 1 cut(s) 197
SspMI CTAG 1 cut(s) 1050
SstI GAGCTC 1 cut(s) 1104
StuI AGGCCT 1 cut(s) 674
StyD4I CCNGG 2 cut(s) 717, 1095
StyI CCWWGG 1 cut(s) 70
TaaI ACNGT 1 cut(s) 953
TaiI ACGT 6 cut(s) 66, 147, 219, 387, 467, 568
TaqI TCGA 3 cut(s) 135, 315, 696
TasI AATT 5 cut(s) 19, 50, 321, 606, 931
TauI GCSGC 2 cut(s) 186, 203
TfiI GAWTC 1 cut(s) 821
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 4 cut(s) 771, 912, 958, 1041
TseFI GTSAC 2 cut(s) 217, 1042
TseI GCWGC 4 cut(s) 75, 299, 584, 794
Tsp45I GTSAC 2 cut(s) 217, 1042
TspDTI ATGAA 5 cut(s) 333, 599, 767, 915, 1218
TspRI CASTG 4 cut(s) 771, 912, 958, 1041
VneI GTGCAC 1 cut(s) 206
XapI RAATTY 3 cut(s) 19, 321, 606
XceI RCATGY 2 cut(s) 112, 274
XmiI GTMKAC 1 cut(s) 437
XspI CTAG 1 cut(s) 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.