Rmu_sc0012468.1_g000026

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012468.1
OG0000309
Physical Location & Seq
Reverse (-)
83844 .. 85053
1210 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012468.1_g000026.1.cds

Sequence Viewer

Length: 924 bp
atggcatccaaatctaatctttctctcttcactgtcactctcctcgcccttcttactctccactcttatgcaattgcttctcatccccacaacaagagctcttccccatttgagttccttgagcatctcaagggttgccacaaaggtgatgaggtcaagggcatcaacgatctcaaaaaatacctagaaaagtacggttacttgaactacaagaaccacagtcattcggacgacgatgattttgatgaccttttggaggaagccatcaagacttaccaggtcaattaccacctcaagtccaccggaacactggacggcaaaacagtgtcgaagatgatgacacctcgttgtggcgtgccggatatcatcaatggcacctcgtccatgagatcagccaagaaaaggcaccaccctgattcaattcataccactgctcactactcatttttccaaggaaatccaaaatggccttcttctaaataccatctcacttacggttttctccccaacaccccatccgaagccacgggcgctgtctcacgggctttcgcaacatgggctggaaacactcacttcacgttcagccagtcccaaagttatgagaatgcgggtttgaagattagttttcataagggtgatcatggagacgggaattcatttgatggcccgggcggggtcctagcccatgcttttagcccgtcggatggaagattccactacgacgcagatgagccttggtctgtgggtgctacaccaggtgattatgacttggagactgtggctttgcatgaaatagggcaccttctgggacttggacatagctcagtggagggagctatcatggccccaactattcccccgggagtgacgaaaagtttgcatggggacgatgttcaaggaatcaaggctttatacaacgtctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

307

Amino Acids

33.56

Weight (kDa)

6.32

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 374, 405, 798
AciI CCGC 2 cut(s) 608, 672
AcsI RAATTY 1 cut(s) 652
AdeI CACNNNGTG 1 cut(s) 758
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 5 cut(s) 256, 350, 402, 673, 704
AgsI TTSAA 4 cut(s) 205, 420, 616, 896
AjnI CCWGG 2 cut(s) 276, 754
AjuI GAANNNNNNNTTGG 2 cut(s) 454, 486
AleI CACNNNNGTG 1 cut(s) 144
AluBI AGCT 3 cut(s) 99, 822, 836
AluI AGCT 3 cut(s) 99, 822, 836
Alw21I GWGCWC 1 cut(s) 101
Alw26I GTCTC 3 cut(s) 541, 639, 767
Ama87I CYCGRG 2 cut(s) 667, 859
AoxI GGCC 3 cut(s) 467, 664, 843
ApoI RAATTY 1 cut(s) 652
ArsI GACNNNNNNTTYG 6 cut(s) 224, 256, 766, 798, 859, 891
AspLEI GCGC 1 cut(s) 533
AspS9I GGNCC 3 cut(s) 665, 676, 844
AsuC2I CCSGG 4 cut(s) 668, 669, 860, 861
AsuHPI GGTGA 3 cut(s) 158, 647, 770
AvaI CYCGRG 2 cut(s) 667, 859
AvaII GGWCC 1 cut(s) 676
BaeGI GKGCMC 1 cut(s) 801
BanI GGYRCC 3 cut(s) 374, 405, 798
BanII GRGCYC 1 cut(s) 101
Bbv12I GWGCWC 1 cut(s) 101
BccI CCATC 5 cut(s) 272, 492, 523, 656, 698
BceAI ACGGC 1 cut(s) 331
BcgI CGANNNNNNTGC 2 cut(s) 859, 893
BciT130I CCWGG 2 cut(s) 278, 756
BclI TGATCA 1 cut(s) 637
BcnI CCSGG 4 cut(s) 668, 669, 860, 861
BcoDI GTCTC 3 cut(s) 541, 639, 767
BfaI CTAG 2 cut(s) 185, 680
BfoI RGCGCY 1 cut(s) 534
Bme1390I CCNGG 6 cut(s) 278, 668, 669, 756, 860, 861
Bme18I GGWCC 1 cut(s) 676
BmeT110I CYCGRG 2 cut(s) 667, 859
BmgT120I GGNCC 3 cut(s) 665, 676, 844
BmiI GGNNCC 5 cut(s) 376, 407, 677, 800, 846
BmrFI CCNGG 6 cut(s) 278, 668, 669, 756, 860, 861
BmsI GCATC 3 cut(s) 14, 133, 171
BpuEI CTTGAG 3 cut(s) 113, 140, 278
BpuMI CCSGG 4 cut(s) 668, 669, 860, 861
BsaJI CCNNGG 6 cut(s) 451, 525, 667, 734, 858, 859
BsaWI WCCGGW 1 cut(s) 302
Bsc4I CCNNNNNNNGG 5 cut(s) 256, 350, 402, 673, 704
Bse1I ACTGG 2 cut(s) 315, 586
BseBI CCWGG 2 cut(s) 278, 756
BseDI CCNNGG 6 cut(s) 451, 525, 667, 734, 858, 859
BseGI GGATG 4 cut(s) 5, 82, 515, 709
BseLI CCNNNNNNNGG 5 cut(s) 256, 350, 402, 673, 704
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 2 cut(s) 315, 586
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 801
BshFI GGCC 3 cut(s) 469, 666, 845
BshNI GGYRCC 3 cut(s) 374, 405, 798
BsiHKAI GWGCWC 1 cut(s) 101
BsiHKCI CYCGRG 2 cut(s) 667, 859
BsiSI CCGG 4 cut(s) 303, 359, 668, 860
BslFI GGGAC 3 cut(s) 574, 822, 899
BslI CCNNNNNNNGG 5 cut(s) 256, 350, 402, 673, 704
BsmAI GTCTC 3 cut(s) 541, 639, 767
BsmBI CGTCTC 1 cut(s) 639
BsmFI GGGAC 3 cut(s) 574, 822, 899
BsmI GAATGC 1 cut(s) 610
BsnI GGCC 3 cut(s) 469, 666, 845
BsoBI CYCGRG 2 cut(s) 667, 859
Bsp1286I GDGCHC 2 cut(s) 101, 801
Bsp143I GATC 3 cut(s) 169, 389, 637
BspACI CCGC 2 cut(s) 608, 672
BspANI GGCC 3 cut(s) 469, 666, 845
BspCNI CTCAG 1 cut(s) 836
BspLI GGNNCC 5 cut(s) 376, 407, 677, 800, 846
BspQI GCTCTTC 1 cut(s) 106
BspT107I GGYRCC 3 cut(s) 374, 405, 798
BsrI ACTGG 2 cut(s) 315, 586
BssECI CCNNGG 6 cut(s) 451, 525, 667, 734, 858, 859
BssMI GATC 3 cut(s) 169, 389, 637
BssT1I CCWWGG 2 cut(s) 451, 734
Bst2UI CCWGG 2 cut(s) 278, 756
Bst4CI ACNGT 6 cut(s) 34, 197, 221, 325, 497, 778
Bst6I CTCTTC 2 cut(s) 32, 106
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 1 cut(s) 823
BstDSI CCRYGG 1 cut(s) 525
BstENI CCTNNNNNAGG 1 cut(s) 254
BstF5I GGATG 4 cut(s) 5, 82, 515, 709
BstH2I RGCGCY 1 cut(s) 534
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 3 cut(s) 172, 392, 640
BstMAI GTCTC 3 cut(s) 541, 639, 767
BstMBI GATC 3 cut(s) 169, 389, 637
BstMWI GCNNNNNNNGC 3 cut(s) 530, 557, 842
BstNI CCWGG 2 cut(s) 278, 756
BstSCI CCNGG 6 cut(s) 276, 666, 667, 754, 858, 859
BstSLI GKGCMC 1 cut(s) 801
BsuRI GGCC 3 cut(s) 469, 666, 845
BtgI CCRYGG 1 cut(s) 525
BtsCI GGATG 4 cut(s) 5, 82, 515, 709
BtsI GCAGTG 1 cut(s) 429
BtsIMutI CAGTG 5 cut(s) 30, 308, 330, 429, 831
Cac8I GCNNGC 1 cut(s) 356
CfoI GCGC 1 cut(s) 533
Cfr13I GGNCC 3 cut(s) 665, 676, 844
Cfr9I CCCGGG 2 cut(s) 667, 859
CseI GACGC 1 cut(s) 731
CsiI ACCWGGT 2 cut(s) 276, 754
Csp6I GTAC 1 cut(s) 193
CviAII CATG 7 cut(s) 385, 555, 641, 686, 788, 841, 881
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 823
DpnI GATC 3 cut(s) 171, 391, 639
DpnII GATC 3 cut(s) 169, 389, 637
DraIII CACNNNGTG 1 cut(s) 758
Eam1104I CTCTTC 2 cut(s) 32, 106
EarI CTCTTC 2 cut(s) 32, 106
Ecl136II GAGCTC 1 cut(s) 99
Eco130I CCWWGG 2 cut(s) 451, 734
Eco24I GRGCYC 1 cut(s) 101
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 1 cut(s) 676
Eco53kI GAGCTC 1 cut(s) 99
Eco88I CYCGRG 2 cut(s) 667, 859
EcoICRI GAGCTC 1 cut(s) 99
EcoNI CCTNNNNNAGG 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 676
EcoRI GAATTC 1 cut(s) 652
EcoRII CCWGG 2 cut(s) 276, 754
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 2 cut(s) 451, 734
EcoT38I GRGCYC 1 cut(s) 101
ErhI CCWWGG 2 cut(s) 451, 734
Esp3I CGTCTC 1 cut(s) 639
FaeI CATG 7 cut(s) 388, 558, 644, 689, 791, 844, 884
FaqI GGGAC 3 cut(s) 574, 822, 899
FatI CATG 7 cut(s) 384, 554, 640, 685, 787, 840, 880
FauI CCCGC 2 cut(s) 601, 665
FbaI TGATCA 1 cut(s) 637
FokI GGATG 3 cut(s) 69, 502, 716
FriOI GRGCYC 1 cut(s) 101
FspBI CTAG 2 cut(s) 185, 680
GlaI GCGC 1 cut(s) 532
HaeII RGCGCY 1 cut(s) 534
HaeIII GGCC 3 cut(s) 469, 666, 845
HapII CCGG 4 cut(s) 303, 359, 668, 860
HgaI GACGC 1 cut(s) 731
HhaI GCGC 1 cut(s) 533
Hin1II CATG 7 cut(s) 388, 558, 644, 689, 791, 844, 884
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HinfI GANTC 3 cut(s) 416, 711, 900
HpaII CCGG 4 cut(s) 303, 359, 668, 860
HphI GGTGA 3 cut(s) 158, 647, 770
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 4 cut(s) 229, 520, 703, 923
Hpy188III TCNNGA 1 cut(s) 268
Hpy8I GTNNAC 1 cut(s) 300
Hpy99I CGWCG 3 cut(s) 236, 703, 725
HpyAV CCTTC 3 cut(s) 59, 480, 812
HpyCH4III ACNGT 6 cut(s) 34, 197, 221, 325, 497, 778
HpyCH4IV ACGT 2 cut(s) 578, 918
HpyCH4V TGCA 3 cut(s) 71, 787, 880
HpyF10VI GCNNNNNNNGC 3 cut(s) 530, 557, 842
HpyF3I CTNAG 1 cut(s) 823
HpySE526I ACGT 2 cut(s) 578, 918
Hsp92II CATG 7 cut(s) 388, 558, 644, 689, 791, 844, 884
HspAI GCGC 1 cut(s) 531
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 3 cut(s) 169, 389, 637
LguI GCTCTTC 1 cut(s) 106
LmnI GCTCC 1 cut(s) 833
LweI GCATC 3 cut(s) 14, 133, 171
MabI ACCWGGT 2 cut(s) 276, 754
MaeI CTAG 2 cut(s) 185, 680
MaeII ACGT 2 cut(s) 578, 918
MaeIII GTNAC 3 cut(s) 34, 197, 865
MalI GATC 3 cut(s) 171, 391, 639
MboI GATC 3 cut(s) 169, 389, 637
MboII GAAGA 6 cut(s) 19, 93, 343, 465, 628, 720
MfeI CAATTG 1 cut(s) 72
MhlI GDGCHC 2 cut(s) 101, 801
MluCI AATT 4 cut(s) 72, 283, 420, 652
MmeI TCCRAC 1 cut(s) 681
MnlI CCTC 7 cut(s) 53, 145, 250, 302, 354, 388, 823
MslI CAYNNNNRTG 3 cut(s) 66, 144, 633
MspI CCGG 4 cut(s) 303, 359, 668, 860
MspR9I CCNGG 6 cut(s) 278, 668, 669, 756, 860, 861
MunI CAATTG 1 cut(s) 72
Mva1269I GAATGC 1 cut(s) 610
MvaI CCWGG 2 cut(s) 278, 756
MwoI GCNNNNNNNGC 3 cut(s) 530, 557, 842
NciI CCSGG 4 cut(s) 668, 669, 860, 861
NdeII GATC 3 cut(s) 169, 389, 637
NlaIII CATG 7 cut(s) 388, 558, 644, 689, 791, 844, 884
NlaIV GGNNCC 5 cut(s) 376, 407, 677, 800, 846
NmuCI GTSAC 2 cut(s) 34, 865
OliI CACNNNNGTG 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 106
PctI GAATGC 1 cut(s) 610
PfeI GAWTC 3 cut(s) 416, 711, 900
PpuMI RGGWCCY 1 cut(s) 676
Psp124BI GAGCTC 1 cut(s) 101
Psp5II RGGWCCY 1 cut(s) 676
Psp6I CCWGG 2 cut(s) 276, 754
PspGI CCWGG 2 cut(s) 276, 754
PspN4I GGNNCC 5 cut(s) 376, 407, 677, 800, 846
PspPI GGNCC 3 cut(s) 665, 676, 844
PspPPI RGGWCCY 1 cut(s) 676
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 3 cut(s) 66, 144, 633
SacI GAGCTC 1 cut(s) 101
SapI GCTCTTC 1 cut(s) 106
Sau3AI GATC 3 cut(s) 169, 389, 637
Sau96I GGNCC 3 cut(s) 665, 676, 844
ScrFI CCNGG 6 cut(s) 278, 668, 669, 756, 860, 861
SduI GDGCHC 2 cut(s) 101, 801
SexAI ACCWGGT 2 cut(s) 276, 754
SfaNI GCATC 3 cut(s) 14, 133, 171
SinI GGWCC 1 cut(s) 676
SmaI CCCGGG 2 cut(s) 669, 861
SmiMI CAYNNNNRTG 3 cut(s) 66, 144, 633
SmlI CTYRAG 3 cut(s) 119, 128, 293
SmoI CTYRAG 3 cut(s) 119, 128, 293
SrfI GCCCGGGC 1 cut(s) 669
Sse9I AATT 4 cut(s) 72, 283, 420, 652
SsiI CCGC 2 cut(s) 608, 672
SspMI CTAG 2 cut(s) 185, 680
SstI GAGCTC 1 cut(s) 101
StyD4I CCNGG 6 cut(s) 276, 666, 667, 754, 858, 859
StyI CCWWGG 2 cut(s) 451, 734
TaaI ACNGT 6 cut(s) 34, 197, 221, 325, 497, 778
TaiI ACGT 2 cut(s) 581, 921
TaqI TCGA 1 cut(s) 329
TasI AATT 4 cut(s) 72, 283, 420, 652
TfiI GAWTC 3 cut(s) 416, 711, 900
TscAI CASTG 5 cut(s) 37, 315, 330, 436, 831
TseFI GTSAC 2 cut(s) 34, 865
Tsp45I GTSAC 2 cut(s) 34, 865
TspDTI ATGAA 4 cut(s) 413, 617, 645, 804
TspMI CCCGGG 2 cut(s) 667, 859
TspRI CASTG 5 cut(s) 37, 315, 330, 436, 831
VpaK11BI GGWCC 1 cut(s) 676
XagI CCTNNNNNAGG 1 cut(s) 254
XapI RAATTY 1 cut(s) 652
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XmaI CCCGGG 2 cut(s) 667, 859
XspI CTAG 2 cut(s) 185, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.