MD09G1040400.v1.1

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000309
Physical Location & Seq
Forward (+)
2545513 .. 2546442
930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1040400.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGCTTCAAAATGTACTCTTTCTCTGTTCACAATCACTTTTCTCTTTGTCCTCCTTTCTCTCTTCTCCCATGCAACGTCGTCAGAAACCCACAACAAAAACACGTCGCCATTCGAGTTTCTCAACCATCTTAAGGGGTGTCACAAGGGCGACAAGGTCCAAGGCATCCAAGACCTAAAGAAGTACCTTGAAAAATTTGGATACTTAAACGGCCATTCCGATAATGATGATTTCGACGACATCTTGGAGTCAGCGATCAAAACTTACCAGCTCAACTACCACCTCAAAGCCACCGGAACATTAGATGACAAAACCGTATCAAAAATGATGATGCCGCGATGCGGTGTGCCAGACATCATCAATGGCACCACCTCCATGCGATCGGGAAAGAAAAGACACCCTCACCACCACAGCGGACATGGTTCGCCTCACACGGTTGCTCATTATTCTTTCTTCCCCGGAAACCCTAAATGGCCTTCTTCTAAGTACCATCTCACCTATGCTTTTGATCAAAGCACCCCAACTGATGCCCGGGACGCAGTTGCGCGCGCTTTTGCAACATGGGCAGGCAACACACACTTCTCGTTCAGTCAATCCCAAACCTATGAGAACGCGGATCTGACAATAAGTTTTGCTAGTGGTGATCATGGAGATGGGAGCCCATTTGAAGGCCGAGGCGGGACCCTTGCTCATGCATTTGCACCGACCAATGGGAGATTCCACTACGATGCCGATGAGCCGTGGGCTGTGGGAGCTGTGGAGGGTGCTTATGACTTGGAGACTGTGGCATTGCATGAAATCGGACATCTGTTGGGGCTACGACATAGCTCTGTTGAAGGAGCTGTGATGTCCTCCACCGTTCGTACTGGATTTGCTCAAAGTTTGCATGCAGATGATATTCAAGGAATTAAAGCTTTATATAATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

310

Amino Acids

33.96

Weight (kDa)

6.41

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 54 - 109 2.8e-12 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 157 - 307 7.3e-46 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 365
AccII CGCG 4 cut(s) 337, 547, 549, 614
AciI CCGC 5 cut(s) 335, 342, 414, 614, 678
AclWI GGATC 1 cut(s) 624
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 194
AfaI GTAC 4 cut(s) 16, 185, 488, 865
AfiI CCNNNNNNNGG 4 cut(s) 133, 341, 668, 710
AflII CTTAAG 1 cut(s) 131
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 5 cut(s) 9, 191, 668, 836, 902
AjiI CACGTC 1 cut(s) 105
AjuI GAANNNNNNNTTGG 2 cut(s) 701, 733
AluBI AGCT 5 cut(s) 271, 755, 828, 842, 914
AluI AGCT 5 cut(s) 271, 755, 828, 842, 914
Alw26I GTCTC 1 cut(s) 773
AlwI GGATC 1 cut(s) 624
Ama87I CYCGRG 1 cut(s) 531
AoxI GGCC 3 cut(s) 211, 473, 670
ApoI RAATTY 1 cut(s) 194
AspLEI GCGC 3 cut(s) 547, 549, 551
AspS9I GGNCC 2 cut(s) 157, 681
AsuC2I CCSGG 3 cut(s) 459, 532, 533
AsuHPI GGTGA 3 cut(s) 395, 487, 653
AvaI CYCGRG 1 cut(s) 531
AvaII GGWCC 2 cut(s) 157, 681
BanI GGYRCC 1 cut(s) 365
BanII GRGCYC 1 cut(s) 662
BccI CCATC 3 cut(s) 135, 498, 647
BceAI ACGGC 2 cut(s) 226, 724
BciVI GTATCC 1 cut(s) 194
BclI TGATCA 2 cut(s) 508, 643
BcnI CCSGG 3 cut(s) 459, 532, 533
BcoDI GTCTC 1 cut(s) 773
BfaI CTAG 1 cut(s) 636
BfrI CTTAAG 1 cut(s) 131
BfuI GTATCC 1 cut(s) 194
BisI GCNGC 1 cut(s) 335
BlsI GCNGC 1 cut(s) 336
Bme1390I CCNGG 3 cut(s) 459, 532, 533
Bme18I GGWCC 2 cut(s) 157, 681
BmeT110I CYCGRG 1 cut(s) 531
BmgBI CACGTC 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 157, 681
BmiI GGNNCC 4 cut(s) 367, 659, 682, 683
BmrFI CCNGG 3 cut(s) 459, 532, 533
BmsI GCATC 5 cut(s) 174, 321, 329, 517, 718
BpuMI CCSGG 3 cut(s) 459, 532, 533
BsaJI CCNNGG 5 cut(s) 160, 457, 531, 673, 740
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 4 cut(s) 133, 341, 668, 710
Bse1I ACTGG 1 cut(s) 871
Bse3DI GCAATG 1 cut(s) 788
BseDI CCNNGG 5 cut(s) 160, 457, 531, 673, 740
BseGI GGATG 1 cut(s) 165
BseLI CCNNNNNNNGG 4 cut(s) 133, 341, 668, 710
BseMI GCAATG 1 cut(s) 788
BseNI ACTGG 1 cut(s) 871
BsePI GCGCGC 2 cut(s) 545, 547
Bsh1236I CGCG 4 cut(s) 337, 547, 549, 614
Bsh1285I CGRYCG 1 cut(s) 383
BshFI GGCC 3 cut(s) 213, 475, 672
BshNI GGYRCC 1 cut(s) 365
BsiEI CGRYCG 1 cut(s) 383
BsiHKCI CYCGRG 1 cut(s) 531
BsiSI CCGG 3 cut(s) 294, 459, 532
BslFI GGGAC 2 cut(s) 548, 694
BslI CCNNNNNNNGG 4 cut(s) 133, 341, 668, 710
BsmAI GTCTC 1 cut(s) 773
BsmFI GGGAC 2 cut(s) 548, 694
BsnI GGCC 3 cut(s) 213, 475, 672
BsoBI CYCGRG 1 cut(s) 531
Bsp1286I GDGCHC 1 cut(s) 662
Bsp143I GATC 5 cut(s) 255, 380, 508, 616, 643
BspACI CCGC 5 cut(s) 335, 342, 414, 614, 678
BspANI GGCC 3 cut(s) 213, 475, 672
BspFNI CGCG 4 cut(s) 337, 547, 549, 614
BspLI GGNNCC 4 cut(s) 367, 659, 682, 683
BspPI GGATC 1 cut(s) 624
BspT107I GGYRCC 1 cut(s) 365
BspTI CTTAAG 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 788
BsrI ACTGG 1 cut(s) 871
BssECI CCNNGG 5 cut(s) 160, 457, 531, 673, 740
BssHII GCGCGC 2 cut(s) 545, 547
BssMI GATC 5 cut(s) 255, 380, 508, 616, 643
BssT1I CCWWGG 1 cut(s) 160
Bst4CI ACNGT 4 cut(s) 316, 436, 784, 859
Bst6I CTCTTC 1 cut(s) 68
BstAFI CTTAAG 1 cut(s) 131
BstC8I GCNNGC 4 cut(s) 547, 549, 568, 888
BstDEI CTNAG 1 cut(s) 483
BstDSI CCRYGG 1 cut(s) 740
BstF5I GGATG 1 cut(s) 165
BstFNI CGCG 4 cut(s) 337, 547, 549, 614
BstHHI GCGC 3 cut(s) 547, 549, 551
BstKTI GATC 5 cut(s) 258, 383, 511, 619, 646
BstMAI GTCTC 1 cut(s) 773
BstMBI GATC 5 cut(s) 255, 380, 508, 616, 643
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 3 cut(s) 536, 563, 752
BstNSI RCATGY 1 cut(s) 890
BstSCI CCNGG 3 cut(s) 457, 530, 531
BstUI CGCG 4 cut(s) 337, 547, 549, 614
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 3 cut(s) 213, 475, 672
BtgI CCRYGG 1 cut(s) 740
BtgZI GCGATG 1 cut(s) 352
BtrI CACGTC 1 cut(s) 105
BtsCI GGATG 1 cut(s) 165
Cac8I GCNNGC 4 cut(s) 547, 549, 568, 888
CfoI GCGC 3 cut(s) 547, 549, 551
Cfr13I GGNCC 2 cut(s) 157, 681
Cfr9I CCCGGG 1 cut(s) 531
CseI GACGC 1 cut(s) 545
Csp6I GTAC 4 cut(s) 15, 184, 487, 864
CviAII CATG 8 cut(s) 71, 376, 419, 561, 647, 692, 794, 887
CviQI GTAC 4 cut(s) 15, 184, 487, 864
DdeI CTNAG 1 cut(s) 483
DpnI GATC 5 cut(s) 257, 382, 510, 618, 645
DpnII GATC 5 cut(s) 255, 380, 508, 616, 643
EaeI YGGCCR 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
Eco130I CCWWGG 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 662
Eco47I GGWCC 2 cut(s) 157, 681
Eco88I CYCGRG 1 cut(s) 531
EcoO109I RGGNCCY 1 cut(s) 681
EcoT14I CCWWGG 1 cut(s) 160
EcoT22I ATGCAT 1 cut(s) 697
EcoT38I GRGCYC 1 cut(s) 662
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 8 cut(s) 74, 379, 422, 564, 650, 695, 797, 890
FaqI GGGAC 2 cut(s) 548, 694
FatI CATG 8 cut(s) 70, 375, 418, 560, 646, 691, 793, 886
FauI CCCGC 1 cut(s) 671
FbaI TGATCA 2 cut(s) 508, 643
Fnu4HI GCNGC 1 cut(s) 335
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 662
Fsp4HI GCNGC 1 cut(s) 335
FspBI CTAG 1 cut(s) 636
GlaI GCGC 3 cut(s) 546, 548, 550
GluI GCNGC 1 cut(s) 335
HaeIII GGCC 3 cut(s) 213, 475, 672
HapII CCGG 3 cut(s) 294, 459, 532
HgaI GACGC 1 cut(s) 545
HhaI GCGC 3 cut(s) 547, 549, 551
Hin1II CATG 8 cut(s) 74, 379, 422, 564, 650, 695, 797, 890
Hin6I GCGC 3 cut(s) 545, 547, 549
HinP1I GCGC 3 cut(s) 545, 547, 549
HindIII AAGCTT 1 cut(s) 912
HinfI GANTC 2 cut(s) 248, 717
HpaII CCGG 3 cut(s) 294, 459, 532
HphI GGTGA 3 cut(s) 395, 487, 653
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 4 cut(s) 85, 220, 621, 803
Hpy188III TCNNGA 1 cut(s) 384
Hpy8I GTNNAC 1 cut(s) 30
Hpy99I CGWCG 3 cut(s) 82, 109, 239
HpyAV CCTTC 3 cut(s) 486, 662, 830
HpyCH4III ACNGT 4 cut(s) 316, 436, 784, 859
HpyCH4IV ACGT 2 cut(s) 77, 104
HpyCH4V TGCA 7 cut(s) 74, 557, 695, 701, 793, 886, 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 563, 752
HpyF3I CTNAG 1 cut(s) 483
HpySE526I ACGT 2 cut(s) 77, 104
Hsp92II CATG 8 cut(s) 74, 379, 422, 564, 650, 695, 797, 890
HspAI GCGC 3 cut(s) 545, 547, 549
KflI GGGWCCC 1 cut(s) 681
Ksp22I TGATCA 2 cut(s) 508, 643
Kzo9I GATC 5 cut(s) 255, 380, 508, 616, 643
LmnI GCTCC 3 cut(s) 657, 752, 839
LpnPI CCDG 7 cut(s) 281, 307, 363, 472, 545, 552, 852
LweI GCATC 5 cut(s) 174, 321, 329, 517, 718
MaeI CTAG 1 cut(s) 636
MaeII ACGT 2 cut(s) 77, 104
MaeIII GTNAC 1 cut(s) 140
MalI GATC 5 cut(s) 257, 382, 510, 618, 645
MboI GATC 5 cut(s) 255, 380, 508, 616, 643
MboII GAAGA 3 cut(s) 55, 445, 471
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 1 cut(s) 662
MluCI AATT 2 cut(s) 194, 906
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 8 cut(s) 62, 293, 382, 411, 438, 668, 754, 862
Mph1103I ATGCAT 1 cut(s) 697
MseI TTAA 3 cut(s) 132, 206, 909
MslI CAYNNNNRTG 4 cut(s) 374, 651, 726, 891
MspA1I CMGCKG 1 cut(s) 414
MspCI CTTAAG 1 cut(s) 131
MspI CCGG 3 cut(s) 294, 459, 532
MspR9I CCNGG 3 cut(s) 459, 532, 533
MvnI CGCG 4 cut(s) 337, 547, 549, 614
MwoI GCNNNNNNNGC 3 cut(s) 536, 563, 752
NciI CCSGG 3 cut(s) 459, 532, 533
NdeII GATC 5 cut(s) 255, 380, 508, 616, 643
NlaIII CATG 8 cut(s) 74, 379, 422, 564, 650, 695, 797, 890
NlaIV GGNNCC 4 cut(s) 367, 659, 682, 683
NmeAIII GCCGAG 1 cut(s) 698
NmuCI GTSAC 1 cut(s) 140
NsiI ATGCAT 1 cut(s) 697
NspI RCATGY 1 cut(s) 890
PaeI GCATGC 1 cut(s) 890
PauI GCGCGC 2 cut(s) 545, 547
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PfeI GAWTC 1 cut(s) 717
PflFI GACNNNGTC 1 cut(s) 155
PkrI GCNGC 1 cut(s) 336
Ple19I CGATCG 1 cut(s) 383
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
PpuMI RGGWCCY 1 cut(s) 681
Psp5II RGGWCCY 1 cut(s) 681
PspN4I GGNNCC 4 cut(s) 367, 659, 682, 683
PspPI GGNCC 2 cut(s) 157, 681
PspPPI RGGWCCY 1 cut(s) 681
PsuI RGATCY 1 cut(s) 616
PsyI GACNNNGTC 1 cut(s) 155
PteI GCGCGC 2 cut(s) 545, 547
PvuI CGATCG 1 cut(s) 383
RsaI GTAC 4 cut(s) 16, 185, 488, 865
RsaNI GTAC 4 cut(s) 15, 184, 487, 864
RseI CAYNNNNRTG 4 cut(s) 374, 651, 726, 891
SaqAI TTAA 3 cut(s) 132, 206, 909
SatI GCNGC 1 cut(s) 335
Sau3AI GATC 5 cut(s) 255, 380, 508, 616, 643
Sau96I GGNCC 2 cut(s) 157, 681
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 3 cut(s) 459, 532, 533
SduI GDGCHC 1 cut(s) 662
SfaNI GCATC 5 cut(s) 174, 321, 329, 517, 718
SinI GGWCC 2 cut(s) 157, 681
SmaI CCCGGG 1 cut(s) 533
SmiMI CAYNNNNRTG 4 cut(s) 374, 651, 726, 891
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
SphI GCATGC 1 cut(s) 890
Sse9I AATT 2 cut(s) 194, 906
SsiI CCGC 5 cut(s) 335, 342, 414, 614, 678
SspMI CTAG 1 cut(s) 636
StyD4I CCNGG 3 cut(s) 457, 530, 531
StyI CCWWGG 1 cut(s) 160
TaaI ACNGT 4 cut(s) 316, 436, 784, 859
TaiI ACGT 2 cut(s) 80, 107
TaqI TCGA 2 cut(s) 114, 234
TasI AATT 2 cut(s) 194, 906
TatI WGTACW 1 cut(s) 14
TauI GCSGC 1 cut(s) 337
TfiI GAWTC 1 cut(s) 717
Tru1I TTAA 3 cut(s) 132, 206, 909
Tru9I TTAA 3 cut(s) 132, 206, 909
TseFI GTSAC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 1 cut(s) 810
TspMI CCCGGG 1 cut(s) 531
Tth111I GACNNNGTC 1 cut(s) 155
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 157, 681
XapI RAATTY 1 cut(s) 194
XceI RCATGY 1 cut(s) 890
XcmI CCANNNNNNNNNTGG 1 cut(s) 416
XmaI CCCGGG 1 cut(s) 531
XspI CTAG 1 cut(s) 636
Zsp2I ATGCAT 1 cut(s) 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.