Rh2BG630000

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000309
Physical Location & Seq
Reverse (-)
85161690 .. 85264221
102532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG630000.1

Sequence Viewer

Length: 930 bp
ATGGCATCCAAATCTAATCTTTTTCTCTTTACTATCACTCTCCTGCTCGTCCTTCTTACTTTCCTCTCTTATGCAACTGCTTCTCATCCCCACAACAAGAGCTCTTCCCCATTTGAGTTCCTTGAACATCTCAAGGGTTGCCACAAAGGTGATAAGGTCAAAGGCATCAATGATCTCAAAAAATACCTTGAAAAGTACGGTTACTTGAACTACAAGAACCACAGTCATTCGGACGATGATGATTTTGACGACCTTTTGGAGGAAGCCATCAAGACTTACCAGATCAATTACCACCTCAAGTCCACCGGAACATTGGACGGCAAAACAGTGTCGAAGATGATGACACCTCGTTGTGGCATGCCGGATATCATCAATGGCACCTCGTCCATGAGATCAGCCAAGAAAAGGCACCACCATGGTTCAATTCATACCACTGTTCACTACTCATTTCCTAATGGAAAACCAAAATGGCCTTCCTCTAAATACCATCTGACATACGCTTTCTTCCCCGGCACCCCATCCCAAGCCACGGGTGCTATAGCACAGGCTTTCGCAACATGGGCTAGAAGCACTCACTTCAAGTTCAGTCAGGCCCAAAATTACCAGAATGCAGATGTCAAGATTAGTTTTCACCGAGGTAATCATGGAGACGGGCGTAATTTTGATGGGCCTGGCGGAGTGCTGGCCCATGCTTTTTACCCTACGGATGGAAGATTCCACTTCGACGCAGCTGAAAATTGGTCCGCGGGTGCTAAACCAGGTGCCTATGACTTGGAGAGTGTTGCTTTGCACGAAATAGGGCACCTTCTGGGACTTGGACATAGCTCAGTGAATGGAGCTGTCATGGCAGCAACCATCTCCCCGGGAGTCACTCTTAAAAGTTTGAACGGGGACGATATCCAAGGAATTAAAGCTTTATACAACGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

309

Amino Acids

34.02

Weight (kDa)

8.95

Isoelectric Point (pI)

25.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 56 - 113 6e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 156 - 307 5.5e-47 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 377, 408, 512, 761, 801
AccII CGCG 1 cut(s) 746
AciI CCGC 3 cut(s) 675, 744, 746
AclI AACGTT 1 cut(s) 924
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 5 cut(s) 259, 353, 405, 529, 707
AgsI TTSAA 6 cut(s) 125, 191, 208, 423, 580, 886
AjnI CCWGG 2 cut(s) 670, 757
AjuI GAANNNNNNNTTGG 2 cut(s) 457, 489
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 5 cut(s) 102, 731, 825, 839, 914
AluI AGCT 5 cut(s) 102, 731, 825, 839, 914
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 642
Ama87I CYCGRG 1 cut(s) 862
AoxI GGCC 4 cut(s) 470, 591, 668, 684
ApeKI GCWGC 2 cut(s) 728, 848
AspS9I GGNCC 4 cut(s) 592, 668, 685, 741
AsuC2I CCSGG 3 cut(s) 510, 863, 864
AsuHPI GGTGA 2 cut(s) 161, 623
AvaI CYCGRG 1 cut(s) 862
AvaII GGWCC 1 cut(s) 741
BaeGI GKGCMC 1 cut(s) 804
BanI GGYRCC 5 cut(s) 377, 408, 512, 761, 801
BanII GRGCYC 1 cut(s) 104
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 2 cut(s) 740, 860
BccI CCATC 6 cut(s) 275, 495, 526, 659, 701, 863
BceAI ACGGC 1 cut(s) 334
BciT130I CCWGG 2 cut(s) 672, 759
BcnI CCSGG 3 cut(s) 510, 863, 864
BcoDI GTCTC 1 cut(s) 642
BfaI CTAG 1 cut(s) 564
BfmI CTRYAG 1 cut(s) 537
BisI GCNGC 2 cut(s) 729, 849
BlsI GCNGC 2 cut(s) 730, 850
Bme1390I CCNGG 5 cut(s) 510, 672, 759, 863, 864
Bme18I GGWCC 1 cut(s) 741
BmeT110I CYCGRG 1 cut(s) 862
BmgT120I GGNCC 4 cut(s) 592, 668, 685, 741
BmiI GGNNCC 5 cut(s) 379, 410, 514, 763, 803
BmrFI CCNGG 5 cut(s) 510, 672, 759, 863, 864
BmsI GCATC 2 cut(s) 14, 174
BpuEI CTTGAG 2 cut(s) 116, 281
BpuMI CCSGG 3 cut(s) 510, 863, 864
BsaJI CCNNGG 8 cut(s) 415, 508, 528, 634, 744, 861, 862, 901
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 353, 405, 529, 707
BseBI CCWGG 2 cut(s) 672, 759
BseDI CCNNGG 8 cut(s) 415, 508, 528, 634, 744, 861, 862, 901
BseGI GGATG 4 cut(s) 5, 85, 518, 712
BseLI CCNNNNNNNGG 5 cut(s) 259, 353, 405, 529, 707
BseMII CTCAG 1 cut(s) 840
BseSI GKGCMC 1 cut(s) 804
BseXI GCAGC 2 cut(s) 740, 860
Bsh1236I CGCG 1 cut(s) 746
BshFI GGCC 4 cut(s) 472, 593, 670, 686
BshNI GGYRCC 5 cut(s) 377, 408, 512, 761, 801
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 1 cut(s) 862
BsiSI CCGG 4 cut(s) 306, 362, 510, 863
BslFI GGGAC 2 cut(s) 825, 905
BslI CCNNNNNNNGG 5 cut(s) 259, 353, 405, 529, 707
BsmAI GTCTC 1 cut(s) 642
BsmBI CGTCTC 1 cut(s) 642
BsmFI GGGAC 2 cut(s) 825, 905
BsmI GAATGC 1 cut(s) 613
BsnI GGCC 4 cut(s) 472, 593, 670, 686
BsoBI CYCGRG 1 cut(s) 862
Bsp1286I GDGCHC 2 cut(s) 104, 804
Bsp143I GATC 3 cut(s) 172, 282, 392
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 3 cut(s) 675, 744, 746
BspANI GGCC 4 cut(s) 472, 593, 670, 686
BspCNI CTCAG 1 cut(s) 839
BspFNI CGCG 1 cut(s) 746
BspLI GGNNCC 5 cut(s) 379, 410, 514, 763, 803
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 5 cut(s) 377, 408, 512, 761, 801
BssECI CCNNGG 8 cut(s) 415, 508, 528, 634, 744, 861, 862, 901
BssMI GATC 3 cut(s) 172, 282, 392
BssT1I CCWWGG 2 cut(s) 415, 901
Bst2UI CCWGG 2 cut(s) 672, 759
Bst4CI ACNGT 4 cut(s) 200, 224, 328, 436
Bst6I CTCTTC 1 cut(s) 109
BstC8I GCNNGC 2 cut(s) 359, 684
BstDEI CTNAG 1 cut(s) 826
BstDSI CCRYGG 3 cut(s) 415, 528, 744
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 4 cut(s) 5, 85, 518, 712
BstFNI CGCG 1 cut(s) 746
BstKTI GATC 3 cut(s) 175, 285, 395
BstMAI GTCTC 1 cut(s) 642
BstMBI GATC 3 cut(s) 172, 282, 392
BstMWI GCNNNNNNNGC 3 cut(s) 533, 560, 845
BstNI CCWGG 2 cut(s) 672, 759
BstNSI RCATGY 1 cut(s) 361
BstSCI CCNGG 5 cut(s) 508, 670, 757, 861, 862
BstSFI CTRYAG 1 cut(s) 537
BstSLI GKGCMC 1 cut(s) 804
BstUI CGCG 1 cut(s) 746
BstV1I GCAGC 2 cut(s) 740, 860
BsuRI GGCC 4 cut(s) 472, 593, 670, 686
BtgI CCRYGG 3 cut(s) 415, 528, 744
BtsCI GGATG 4 cut(s) 5, 85, 518, 712
BtsIMutI CAGTG 3 cut(s) 333, 432, 834
Cac8I GCNNGC 2 cut(s) 359, 684
Cfr13I GGNCC 4 cut(s) 592, 668, 685, 741
Cfr42I CCGCGG 1 cut(s) 747
Cfr9I CCCGGG 1 cut(s) 862
CseI GACGC 1 cut(s) 734
CsiI ACCWGGT 1 cut(s) 757
Csp6I GTAC 1 cut(s) 196
CviAII CATG 7 cut(s) 358, 388, 416, 558, 644, 689, 844
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 826
DpnI GATC 3 cut(s) 174, 284, 394
DpnII GATC 3 cut(s) 172, 282, 392
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
EciI GGCGGA 1 cut(s) 690
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 415, 901
Eco24I GRGCYC 1 cut(s) 104
Eco32I GATATC 2 cut(s) 367, 898
Eco47I GGWCC 1 cut(s) 741
Eco53kI GAGCTC 1 cut(s) 102
Eco88I CYCGRG 1 cut(s) 862
EcoICRI GAGCTC 1 cut(s) 102
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoRII CCWGG 2 cut(s) 670, 757
EcoRV GATATC 2 cut(s) 367, 898
EcoT14I CCWWGG 2 cut(s) 415, 901
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 2 cut(s) 415, 901
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 7 cut(s) 361, 391, 419, 561, 647, 692, 847
FaqI GGGAC 2 cut(s) 825, 905
FatI CATG 7 cut(s) 357, 387, 415, 557, 643, 688, 843
FauI CCCGC 1 cut(s) 739
Fnu4HI GCNGC 2 cut(s) 729, 849
FokI GGATG 3 cut(s) 72, 505, 719
FriOI GRGCYC 1 cut(s) 104
Fsp4HI GCNGC 2 cut(s) 729, 849
FspBI CTAG 1 cut(s) 564
GluI GCNGC 2 cut(s) 729, 849
HaeIII GGCC 4 cut(s) 472, 593, 670, 686
HapII CCGG 4 cut(s) 306, 362, 510, 863
HgaI GACGC 1 cut(s) 734
Hin1II CATG 7 cut(s) 361, 391, 419, 561, 647, 692, 847
HindIII AAGCTT 1 cut(s) 912
HinfI GANTC 2 cut(s) 714, 867
HpaII CCGG 4 cut(s) 306, 362, 510, 863
HphI GGTGA 2 cut(s) 161, 623
Hpy166II GTNNAC 2 cut(s) 303, 439
Hpy188I TCNGA 2 cut(s) 232, 492
Hpy188III TCNNGA 2 cut(s) 271, 619
Hpy8I GTNNAC 2 cut(s) 303, 439
Hpy99I CGWCG 1 cut(s) 728
HpyAV CCTTC 3 cut(s) 62, 483, 815
HpyCH4III ACNGT 4 cut(s) 200, 224, 328, 436
HpyCH4IV ACGT 1 cut(s) 924
HpyCH4V TGCA 3 cut(s) 74, 611, 790
HpyF10VI GCNNNNNNNGC 3 cut(s) 533, 560, 845
HpyF3I CTNAG 1 cut(s) 826
HpySE526I ACGT 1 cut(s) 924
Hsp92II CATG 7 cut(s) 361, 391, 419, 561, 647, 692, 847
KspI CCGCGG 1 cut(s) 747
Kzo9I GATC 3 cut(s) 172, 282, 392
LguI GCTCTTC 1 cut(s) 109
LmnI GCTCC 1 cut(s) 836
Lsp1109I GCAGC 2 cut(s) 740, 860
LweI GCATC 2 cut(s) 14, 174
MabI ACCWGGT 1 cut(s) 757
MaeI CTAG 1 cut(s) 564
MaeII ACGT 1 cut(s) 924
MaeIII GTNAC 2 cut(s) 200, 868
MalI GATC 3 cut(s) 174, 284, 394
MboI GATC 3 cut(s) 172, 282, 392
MboII GAAGA 4 cut(s) 96, 346, 496, 723
MhlI GDGCHC 2 cut(s) 104, 804
MluCI AATT 6 cut(s) 286, 423, 598, 658, 736, 906
MlyI GAGTC 1 cut(s) 876
MnlI CCTC 7 cut(s) 74, 253, 305, 357, 391, 487, 629
MseI TTAA 2 cut(s) 876, 909
MslI CAYNNNNRTG 2 cut(s) 147, 414
MspA1I CMGCKG 2 cut(s) 731, 746
MspI CCGG 4 cut(s) 306, 362, 510, 863
MspR9I CCNGG 5 cut(s) 510, 672, 759, 863, 864
Mva1269I GAATGC 1 cut(s) 613
MvaI CCWGG 2 cut(s) 672, 759
MvnI CGCG 1 cut(s) 746
MwoI GCNNNNNNNGC 3 cut(s) 533, 560, 845
NciI CCSGG 3 cut(s) 510, 863, 864
NcoI CCATGG 1 cut(s) 415
NdeII GATC 3 cut(s) 172, 282, 392
NlaIII CATG 7 cut(s) 361, 391, 419, 561, 647, 692, 847
NlaIV GGNNCC 5 cut(s) 379, 410, 514, 763, 803
NmuCI GTSAC 1 cut(s) 868
NspI RCATGY 1 cut(s) 361
OliI CACNNNNGTG 1 cut(s) 147
PaeI GCATGC 1 cut(s) 361
PciSI GCTCTTC 1 cut(s) 109
PctI GAATGC 1 cut(s) 613
PfeI GAWTC 1 cut(s) 714
PkrI GCNGC 2 cut(s) 730, 850
PleI GAGTC 1 cut(s) 875
PpsI GAGTC 1 cut(s) 875
Psp124BI GAGCTC 1 cut(s) 104
Psp1406I AACGTT 1 cut(s) 924
Psp6I CCWGG 2 cut(s) 670, 757
PspGI CCWGG 2 cut(s) 670, 757
PspN4I GGNNCC 5 cut(s) 379, 410, 514, 763, 803
PspPI GGNCC 4 cut(s) 592, 668, 685, 741
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 2 cut(s) 147, 414
SacI GAGCTC 1 cut(s) 104
SacII CCGCGG 1 cut(s) 747
SapI GCTCTTC 1 cut(s) 109
SaqAI TTAA 2 cut(s) 876, 909
SatI GCNGC 2 cut(s) 729, 849
Sau3AI GATC 3 cut(s) 172, 282, 392
Sau96I GGNCC 4 cut(s) 592, 668, 685, 741
SchI GAGTC 1 cut(s) 876
ScrFI CCNGG 5 cut(s) 510, 672, 759, 863, 864
SduI GDGCHC 2 cut(s) 104, 804
SexAI ACCWGGT 1 cut(s) 757
SfaNI GCATC 2 cut(s) 14, 174
SfcI CTRYAG 1 cut(s) 537
Sfr303I CCGCGG 1 cut(s) 747
SgrBI CCGCGG 1 cut(s) 747
SinI GGWCC 1 cut(s) 741
SmaI CCCGGG 1 cut(s) 864
SmiMI CAYNNNNRTG 2 cut(s) 147, 414
SmlI CTYRAG 2 cut(s) 131, 296
SmoI CTYRAG 2 cut(s) 131, 296
SphI GCATGC 1 cut(s) 361
Sse9I AATT 6 cut(s) 286, 423, 598, 658, 736, 906
SsiI CCGC 3 cut(s) 675, 744, 746
SspMI CTAG 1 cut(s) 564
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 5 cut(s) 508, 670, 757, 861, 862
StyI CCWWGG 2 cut(s) 415, 901
TaaI ACNGT 4 cut(s) 200, 224, 328, 436
TaiI ACGT 1 cut(s) 927
TaqI TCGA 2 cut(s) 332, 723
TasI AATT 6 cut(s) 286, 423, 598, 658, 736, 906
TfiI GAWTC 1 cut(s) 714
Tru1I TTAA 2 cut(s) 876, 909
Tru9I TTAA 2 cut(s) 876, 909
TscAI CASTG 3 cut(s) 333, 439, 834
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 2 cut(s) 728, 848
Tsp45I GTSAC 1 cut(s) 868
TspDTI ATGAA 1 cut(s) 416
TspGWI ACGGA 1 cut(s) 719
TspMI CCCGGG 1 cut(s) 862
TspRI CASTG 3 cut(s) 333, 439, 834
VpaK11BI GGWCC 1 cut(s) 741
XagI CCTNNNNNAGG 1 cut(s) 257
XceI RCATGY 1 cut(s) 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 310
XmaI CCCGGG 1 cut(s) 862
XspI CTAG 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.