Rh2AG631600

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000309
Physical Location & Seq
Reverse (-)
85786355 .. 85787969
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG631600.1

Sequence Viewer

Length: 933 bp
ATGGCATCCAAATCTAATATATCTTCTGTCCTCACTATCACTCTCCTCCTCGCCATTCTTACTCTCCTCTCTTATGCAACTGCTTCTCATCCCCTCAGCAAGAGCTCTTCCCCATTTGAGTTCCTTGAGCATCTCAAGGGTTGTCACAAGGGTGATAAGGTCAAAGGCATCAATGACCTCAAGAAATACTTTGAAAAGTACGGTTACTTGAACTACAAGAACCACAGTCATTCGGACGACGATGATTTCGACGACCTTTTGGAGGACGCCGTGAAGACTTACCAGATCAATTACCACCTCAAGGCCACCGGAACATTGGACGGCAAAACAGTGTCAAAAATGATGACGCCTCGTTGTGGCGTGCCGGATATCATCAATGGCACCACGTCCATGCGATCAGCCAAGAAAAGGCACCACCATGGTTCAATTCATACCACTGCTCACTACTTATTTCCTGGTGGAAATCTAAAATGGCCTTCCTCTAAATACCATCTCACCTACGGTTTTCTCCCCGGCACCCGATCCGACGCCACGGCTGCTGTCGCACGGGCTTTCGCAACATGGGCTAGGAACACTCACTTCACGTTCAGCCAGGCCCAAAGTTACGAAAATGCGGATTTAAAGATTAGTTTTCATAAGGGTGATCATGGAGACCGGAGTTCATTTGATGGACCGGGCGGGATCCTAGCCCATGCTTTTTACCCGTCGGATGGAAGATTCCACTACGACGCAGATGAGACTTGGTCTGTGGGTGCTACTCCAGGTGCTATGGACTTGGAGAGTGTGGCTTTGCATGAAATAGGGCACCTTCTGGGACTTGGACATAGCTCAGTGGAGGGAGCTATCATGGCCCCAGCTATTTCCCCGGGAGTTGCTCGAATAAGTTTGACTGGGGACGATGTTCAAGGAATAAGGGCTCTATACAACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

310

Amino Acids

33.83

Weight (kDa)

7.06

Isoelectric Point (pI)

33.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 57 - 114 2.3e-09 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 157 - 308 1e-47 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 380, 411, 515, 804
AciI CCGC 2 cut(s) 614, 678
AclWI GGATC 3 cut(s) 516, 676, 689
AcyI GRCGYC 3 cut(s) 267, 347, 528
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 5 cut(s) 262, 301, 356, 408, 710
AgsI TTSAA 4 cut(s) 194, 211, 426, 905
AjiI CACGTC 1 cut(s) 387
AjnI CCWGG 3 cut(s) 454, 591, 760
AleI CACNNNNGTG 1 cut(s) 150
AluBI AGCT 4 cut(s) 105, 828, 842, 857
AluI AGCT 4 cut(s) 105, 828, 842, 857
Alw21I GWGCWC 1 cut(s) 107
Alw26I GTCTC 2 cut(s) 645, 731
AlwI GGATC 3 cut(s) 516, 676, 689
Ama87I CYCGRG 1 cut(s) 865
AoxI GGCC 4 cut(s) 303, 473, 594, 849
ApeKI GCWGC 1 cut(s) 536
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AspS9I GGNCC 3 cut(s) 595, 671, 850
AsuC2I CCSGG 4 cut(s) 513, 675, 866, 867
AsuHPI GGTGA 3 cut(s) 164, 487, 653
AvaI CYCGRG 1 cut(s) 865
AvaII GGWCC 1 cut(s) 671
BaeGI GKGCMC 1 cut(s) 807
BamHI GGATCC 1 cut(s) 681
BanI GGYRCC 4 cut(s) 380, 411, 515, 804
BanII GRGCYC 2 cut(s) 107, 919
BbsI GAAGAC 1 cut(s) 281
Bbv12I GWGCWC 1 cut(s) 107
BbvCI CCTCAGC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 523
BccI CCATC 3 cut(s) 498, 662, 704
BceAI ACGGC 3 cut(s) 254, 337, 549
BciT130I CCWGG 3 cut(s) 456, 593, 762
BclI TGATCA 1 cut(s) 643
BcnI CCSGG 4 cut(s) 513, 675, 866, 867
BcoDI GTCTC 2 cut(s) 645, 731
BfaI CTAG 2 cut(s) 567, 686
BisI GCNGC 1 cut(s) 537
BlsI GCNGC 1 cut(s) 538
Bme1390I CCNGG 7 cut(s) 456, 513, 593, 675, 762, 866, 867
Bme18I GGWCC 1 cut(s) 671
BmeT110I CYCGRG 1 cut(s) 865
BmgBI CACGTC 1 cut(s) 387
BmgT120I GGNCC 3 cut(s) 595, 671, 850
BmiI GGNNCC 6 cut(s) 382, 413, 517, 683, 806, 852
BmrFI CCNGG 7 cut(s) 456, 513, 593, 675, 762, 866, 867
BmrI ACTGGG 1 cut(s) 900
BmsI GCATC 3 cut(s) 14, 139, 177
BmuI ACTGGG 1 cut(s) 900
BpiI GAAGAC 1 cut(s) 281
BpmI CTGGAG 1 cut(s) 744
Bpu10I CCTNAGC 1 cut(s) 95
BpuEI CTTGAG 4 cut(s) 119, 146, 164, 284
BpuMI CCSGG 4 cut(s) 513, 675, 866, 867
BsaHI GRCGYC 3 cut(s) 267, 347, 528
BsaI GGTCTC 1 cut(s) 645
BsaJI CCNNGG 5 cut(s) 418, 511, 531, 864, 865
BsaWI WCCGGW 2 cut(s) 308, 654
Bsc4I CCNNNNNNNGG 5 cut(s) 262, 301, 356, 408, 710
Bse1I ACTGG 1 cut(s) 895
BseBI CCWGG 3 cut(s) 456, 593, 762
BseDI CCNNGG 5 cut(s) 418, 511, 531, 864, 865
BseGI GGATG 3 cut(s) 5, 88, 715
BseLI CCNNNNNNNGG 5 cut(s) 262, 301, 356, 408, 710
BseMII CTCAG 2 cut(s) 109, 843
BseNI ACTGG 1 cut(s) 895
BseRI GAGGAG 3 cut(s) 35, 38, 56
BseSI GKGCMC 1 cut(s) 807
BseXI GCAGC 1 cut(s) 523
BseYI CCCAGC 1 cut(s) 853
BshFI GGCC 4 cut(s) 305, 475, 596, 851
BshNI GGYRCC 4 cut(s) 380, 411, 515, 804
BsiHKAI GWGCWC 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 865
BsiSI CCGG 6 cut(s) 309, 365, 513, 655, 674, 866
BslFI GGGAC 2 cut(s) 828, 908
BslI CCNNNNNNNGG 5 cut(s) 262, 301, 356, 408, 710
BsmAI GTCTC 2 cut(s) 645, 731
BsmFI GGGAC 2 cut(s) 828, 908
BsnI GGCC 4 cut(s) 305, 475, 596, 851
Bso31I GGTCTC 1 cut(s) 645
BsoBI CYCGRG 1 cut(s) 865
Bsp1286I GDGCHC 3 cut(s) 107, 807, 919
Bsp143I GATC 5 cut(s) 285, 395, 521, 643, 681
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 2 cut(s) 614, 678
BspANI GGCC 4 cut(s) 305, 475, 596, 851
BspCNI CTCAG 2 cut(s) 108, 842
BspLI GGNNCC 6 cut(s) 382, 413, 517, 683, 806, 852
BspPI GGATC 3 cut(s) 516, 676, 689
BspQI GCTCTTC 1 cut(s) 112
BspT107I GGYRCC 4 cut(s) 380, 411, 515, 804
BspTNI GGTCTC 1 cut(s) 645
BsrI ACTGG 1 cut(s) 895
BssECI CCNNGG 5 cut(s) 418, 511, 531, 864, 865
BssMI GATC 5 cut(s) 285, 395, 521, 643, 681
BssNI GRCGYC 3 cut(s) 267, 347, 528
BssT1I CCWWGG 1 cut(s) 418
Bst2UI CCWGG 3 cut(s) 456, 593, 762
Bst4CI ACNGT 4 cut(s) 203, 227, 331, 503
Bst6I CTCTTC 1 cut(s) 112
BstACI GRCGYC 3 cut(s) 267, 347, 528
BstC8I GCNNGC 1 cut(s) 362
BstDEI CTNAG 2 cut(s) 95, 829
BstDSI CCRYGG 2 cut(s) 418, 531
BstENI CCTNNNNNAGG 1 cut(s) 260
BstF5I GGATG 3 cut(s) 5, 88, 715
BstKTI GATC 5 cut(s) 288, 398, 524, 646, 684
BstMAI GTCTC 2 cut(s) 645, 731
BstMBI GATC 5 cut(s) 285, 395, 521, 643, 681
BstMWI GCNNNNNNNGC 4 cut(s) 536, 542, 563, 848
BstNI CCWGG 3 cut(s) 456, 593, 762
BstSCI CCNGG 7 cut(s) 454, 511, 591, 673, 760, 864, 865
BstSLI GKGCMC 1 cut(s) 807
BstV1I GCAGC 1 cut(s) 523
BstV2I GAAGAC 1 cut(s) 281
BstX2I RGATCY 1 cut(s) 681
BstYI RGATCY 1 cut(s) 681
BsuRI GGCC 4 cut(s) 305, 475, 596, 851
BtgI CCRYGG 2 cut(s) 418, 531
BtrI CACGTC 1 cut(s) 387
BtsCI GGATG 3 cut(s) 5, 88, 715
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 3 cut(s) 336, 435, 837
Cac8I GCNNGC 1 cut(s) 362
Cfr13I GGNCC 3 cut(s) 595, 671, 850
Cfr9I CCCGGG 1 cut(s) 865
CseI GACGC 4 cut(s) 275, 355, 536, 737
Csp6I GTAC 1 cut(s) 199
CviAII CATG 7 cut(s) 391, 419, 561, 647, 692, 794, 847
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 2 cut(s) 95, 829
DpnI GATC 5 cut(s) 287, 397, 523, 645, 683
DpnII GATC 5 cut(s) 285, 395, 521, 643, 681
DraI TTTAAA 1 cut(s) 621
Eam1104I CTCTTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 112
Ecl136II GAGCTC 1 cut(s) 105
Eco130I CCWWGG 1 cut(s) 418
Eco24I GRGCYC 2 cut(s) 107, 919
Eco31I GGTCTC 1 cut(s) 645
Eco32I GATATC 1 cut(s) 370
Eco47I GGWCC 1 cut(s) 671
Eco53kI GAGCTC 1 cut(s) 105
Eco88I CYCGRG 1 cut(s) 865
EcoICRI GAGCTC 1 cut(s) 105
EcoNI CCTNNNNNAGG 1 cut(s) 260
EcoRII CCWGG 3 cut(s) 454, 591, 760
EcoRV GATATC 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 418
EcoT38I GRGCYC 2 cut(s) 107, 919
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 7 cut(s) 394, 422, 564, 650, 695, 797, 850
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 2 cut(s) 828, 908
FatI CATG 7 cut(s) 390, 418, 560, 646, 691, 793, 846
FauI CCCGC 1 cut(s) 671
FbaI TGATCA 1 cut(s) 643
Fnu4HI GCNGC 1 cut(s) 537
FokI GGATG 2 cut(s) 75, 722
FriOI GRGCYC 2 cut(s) 107, 919
Fsp4HI GCNGC 1 cut(s) 537
FspBI CTAG 2 cut(s) 567, 686
GluI GCNGC 1 cut(s) 537
GsaI CCCAGC 1 cut(s) 857
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 4 cut(s) 305, 475, 596, 851
HapII CCGG 6 cut(s) 309, 365, 513, 655, 674, 866
HgaI GACGC 4 cut(s) 275, 355, 536, 737
Hin1I GRCGYC 3 cut(s) 267, 347, 528
Hin1II CATG 7 cut(s) 394, 422, 564, 650, 695, 797, 850
HinfI GANTC 1 cut(s) 717
HpaII CCGG 6 cut(s) 309, 365, 513, 655, 674, 866
HphI GGTGA 3 cut(s) 164, 487, 653
Hpy188I TCNGA 4 cut(s) 235, 526, 709, 932
Hpy188III TCNNGA 1 cut(s) 181
Hpy99I CGWCG 5 cut(s) 242, 254, 530, 709, 731
HpyAV CCTTC 2 cut(s) 486, 818
HpyCH4III ACNGT 4 cut(s) 203, 227, 331, 503
HpyCH4IV ACGT 3 cut(s) 386, 584, 927
HpyCH4V TGCA 2 cut(s) 77, 793
HpyF10VI GCNNNNNNNGC 4 cut(s) 536, 542, 563, 848
HpyF3I CTNAG 2 cut(s) 95, 829
HpySE526I ACGT 3 cut(s) 386, 584, 927
Hsp92I GRCGYC 3 cut(s) 267, 347, 528
Hsp92II CATG 7 cut(s) 394, 422, 564, 650, 695, 797, 850
Ksp22I TGATCA 1 cut(s) 643
Kzo9I GATC 5 cut(s) 285, 395, 521, 643, 681
LguI GCTCTTC 1 cut(s) 112
LmnI GCTCC 1 cut(s) 839
Lsp1109I GCAGC 1 cut(s) 523
LweI GCATC 3 cut(s) 14, 139, 177
MaeI CTAG 2 cut(s) 567, 686
MaeII ACGT 3 cut(s) 386, 584, 927
MaeIII GTNAC 3 cut(s) 143, 203, 602
MalI GATC 5 cut(s) 287, 397, 523, 645, 683
MboI GATC 5 cut(s) 285, 395, 521, 643, 681
MboII GAAGA 4 cut(s) 15, 99, 286, 726
MflI RGATCY 1 cut(s) 681
MhlI GDGCHC 3 cut(s) 107, 807, 919
MluCI AATT 2 cut(s) 289, 426
MmeI TCCRAC 2 cut(s) 549, 687
MseI TTAA 1 cut(s) 620
MslI CAYNNNNRTG 4 cut(s) 150, 389, 417, 639
MspI CCGG 6 cut(s) 309, 365, 513, 655, 674, 866
MspR9I CCNGG 7 cut(s) 456, 513, 593, 675, 762, 866, 867
MvaI CCWGG 3 cut(s) 456, 593, 762
MwoI GCNNNNNNNGC 4 cut(s) 536, 542, 563, 848
NciI CCSGG 4 cut(s) 513, 675, 866, 867
NcoI CCATGG 1 cut(s) 418
NdeII GATC 5 cut(s) 285, 395, 521, 643, 681
NlaIII CATG 7 cut(s) 394, 422, 564, 650, 695, 797, 850
NlaIV GGNNCC 6 cut(s) 382, 413, 517, 683, 806, 852
NmuCI GTSAC 1 cut(s) 143
OliI CACNNNNGTG 1 cut(s) 150
PciSI GCTCTTC 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PfeI GAWTC 1 cut(s) 717
PflFI GACNNNGTC 1 cut(s) 742
PkrI GCNGC 1 cut(s) 538
Psp124BI GAGCTC 1 cut(s) 107
Psp6I CCWGG 3 cut(s) 454, 591, 760
PspFI CCCAGC 1 cut(s) 853
PspGI CCWGG 3 cut(s) 454, 591, 760
PspN4I GGNNCC 6 cut(s) 382, 413, 517, 683, 806, 852
PspPI GGNCC 3 cut(s) 595, 671, 850
PsuI RGATCY 1 cut(s) 681
PsyI GACNNNGTC 1 cut(s) 742
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 4 cut(s) 150, 389, 417, 639
SacI GAGCTC 1 cut(s) 107
SapI GCTCTTC 1 cut(s) 112
SaqAI TTAA 1 cut(s) 620
SatI GCNGC 1 cut(s) 537
Sau3AI GATC 5 cut(s) 285, 395, 521, 643, 681
Sau96I GGNCC 3 cut(s) 595, 671, 850
ScrFI CCNGG 7 cut(s) 456, 513, 593, 675, 762, 866, 867
SduI GDGCHC 3 cut(s) 107, 807, 919
SfaNI GCATC 3 cut(s) 14, 139, 177
SinI GGWCC 1 cut(s) 671
SmaI CCCGGG 1 cut(s) 867
SmiMI CAYNNNNRTG 4 cut(s) 150, 389, 417, 639
SmlI CTYRAG 4 cut(s) 125, 134, 179, 299
SmoI CTYRAG 4 cut(s) 125, 134, 179, 299
Sse9I AATT 2 cut(s) 289, 426
SsiI CCGC 2 cut(s) 614, 678
SspMI CTAG 2 cut(s) 567, 686
SstI GAGCTC 1 cut(s) 107
StyD4I CCNGG 7 cut(s) 454, 511, 591, 673, 760, 864, 865
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 4 cut(s) 203, 227, 331, 503
TaiI ACGT 3 cut(s) 389, 587, 930
TaqI TCGA 2 cut(s) 249, 877
TasI AATT 2 cut(s) 289, 426
TfiI GAWTC 1 cut(s) 717
Tru1I TTAA 1 cut(s) 620
Tru9I TTAA 1 cut(s) 620
TscAI CASTG 3 cut(s) 336, 442, 837
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 1 cut(s) 536
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 4 cut(s) 419, 623, 651, 810
TspMI CCCGGG 1 cut(s) 865
TspRI CASTG 3 cut(s) 336, 442, 837
Tth111I GACNNNGTC 1 cut(s) 742
VpaK11BI GGWCC 1 cut(s) 671
XagI CCTNNNNNAGG 1 cut(s) 260
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XmaI CCCGGG 1 cut(s) 865
XspI CTAG 2 cut(s) 567, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.