RLG00000021930

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000309
Physical Location & Seq
Reverse (-)
79363101 .. 79364893
1793 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021930

Sequence Viewer

Length: 684 bp
ATGGCAGGACTGGTAAAAATGTGCCGTTGGTTTTTCTCACGAGAGATTACTGTGCCGCAAAGGAACCAGAGTCATTCAAGCGACGATGATTTCGACGACCTTTTGGAGGACGCCGTGAAGACTTACCAGATCAATTACCACCTCAAGGCCACCGGAACAATGGACCGCAAAACAGTGTCAAAGATGATGACGCCTCGTTGTGGCGTGCCGGATATCATCAATGGCACCTCGTCCATGCAATCAGCCGAGAAAAGGCACCACCATGGTTCAATTCATACCACTTCTCACTATTCATTTTTCCAGGGAAATCCAAAATGGCCTTCCTCAAAATACCATCTCACTTACAGTTTTTTCCCCAACACCCCATCCGAAGCCATGGGCGCTGTCGCACGGGCTTTCGCAACATGGGCTGGAAACACTCACTTCACGTTCAGCCAGTCCCAAAGTTACGAGAATGCGGATTTGAAGATTAGTTTTCATAAGGATGAGACTTGGTCTGTGGGTGCTACTCCAGGTGCTATGGACTTGGAGAGTGTGGCTTTGCATGAAATAGGGCACCTTCTGGGACTTGGACATAGCTCAGTGAAGGGAGCTATCATGGCCCCAGTTATTCCCTCGGGAGTGGCTCGAATAAGTTTGACTGGGGACGATGTTCAAGGAATAAGGGCTTTATACAACGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

228

Amino Acids

25.1

Weight (kDa)

6.89

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 26 - 62 2.4e-06 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 105 - 161 2.7e-06 Matrixin
Peptidase_M10 PF00413 159 - 225 3.8e-20 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 224, 255, 555
AciI CCGC 3 cut(s) 56, 166, 458
AcyI GRCGYC 2 cut(s) 111, 191
AfiI CCNNNNNNNGG 4 cut(s) 106, 145, 200, 252
AgsI TTSAA 4 cut(s) 78, 270, 466, 656
AjnI CCWGG 2 cut(s) 300, 511
AjuI GAANNNNNNNTTGG 2 cut(s) 304, 336
AluBI AGCT 2 cut(s) 579, 593
AluI AGCT 2 cut(s) 579, 593
Alw26I GTCTC 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 616
AoxI GGCC 3 cut(s) 147, 317, 600
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 2 cut(s) 163, 601
AvaI CYCGRG 1 cut(s) 616
AvaII GGWCC 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 558
BanI GGYRCC 3 cut(s) 224, 255, 555
BauI CACGAG 1 cut(s) 39
BbsI GAAGAC 1 cut(s) 125
BccI CCATC 2 cut(s) 342, 373
BceAI ACGGC 2 cut(s) 9, 98
BciT130I CCWGG 2 cut(s) 302, 513
BcoDI GTCTC 1 cut(s) 482
BfoI RGCGCY 1 cut(s) 384
BisI GCNGC 1 cut(s) 56
BlsI GCNGC 1 cut(s) 57
Bme1390I CCNGG 2 cut(s) 302, 513
Bme18I GGWCC 1 cut(s) 163
BmeT110I CYCGRG 1 cut(s) 616
BmgT120I GGNCC 2 cut(s) 163, 601
BmiI GGNNCC 5 cut(s) 65, 226, 257, 557, 603
BmrFI CCNGG 2 cut(s) 302, 513
BmrI ACTGGG 2 cut(s) 599, 651
BmuI ACTGGG 2 cut(s) 599, 651
BpiI GAAGAC 1 cut(s) 125
BpmI CTGGAG 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 128
BsaHI GRCGYC 2 cut(s) 111, 191
BsaJI CCNNGG 4 cut(s) 262, 301, 375, 615
BsaWI WCCGGW 1 cut(s) 152
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 145, 200, 252
Bse1I ACTGG 4 cut(s) 15, 436, 605, 646
BseBI CCWGG 2 cut(s) 302, 513
BseDI CCNNGG 4 cut(s) 262, 301, 375, 615
BseGI GGATG 2 cut(s) 365, 490
BseLI CCNNNNNNNGG 4 cut(s) 106, 145, 200, 252
BseMII CTCAG 1 cut(s) 594
BseNI ACTGG 4 cut(s) 15, 436, 605, 646
BseSI GKGCMC 1 cut(s) 558
BshFI GGCC 3 cut(s) 149, 319, 602
BshNI GGYRCC 3 cut(s) 224, 255, 555
BsiHKCI CYCGRG 1 cut(s) 616
BsiSI CCGG 2 cut(s) 153, 209
BslFI GGGAC 3 cut(s) 424, 579, 659
BslI CCNNNNNNNGG 4 cut(s) 106, 145, 200, 252
BsmAI GTCTC 1 cut(s) 482
BsmFI GGGAC 3 cut(s) 424, 579, 659
BsmI GAATGC 1 cut(s) 460
BsnI GGCC 3 cut(s) 149, 319, 602
BsoBI CYCGRG 1 cut(s) 616
Bsp1286I GDGCHC 1 cut(s) 558
Bsp143I GATC 1 cut(s) 129
Bsp19I CCATGG 2 cut(s) 262, 375
BspACI CCGC 3 cut(s) 56, 166, 458
BspANI GGCC 3 cut(s) 149, 319, 602
BspCNI CTCAG 1 cut(s) 593
BspLI GGNNCC 5 cut(s) 65, 226, 257, 557, 603
BspT107I GGYRCC 3 cut(s) 224, 255, 555
BsrI ACTGG 4 cut(s) 15, 436, 605, 646
BssECI CCNNGG 4 cut(s) 262, 301, 375, 615
BssMI GATC 1 cut(s) 129
BssNI GRCGYC 2 cut(s) 111, 191
BssSI CACGAG 1 cut(s) 39
BssT1I CCWWGG 2 cut(s) 262, 375
Bst2BI CACGAG 1 cut(s) 39
Bst2UI CCWGG 2 cut(s) 302, 513
Bst4CI ACNGT 3 cut(s) 52, 175, 347
BstACI GRCGYC 2 cut(s) 111, 191
BstC8I GCNNGC 1 cut(s) 206
BstDEI CTNAG 1 cut(s) 580
BstDSI CCRYGG 2 cut(s) 262, 375
BstENI CCTNNNNNAGG 1 cut(s) 104
BstF5I GGATG 2 cut(s) 365, 490
BstH2I RGCGCY 1 cut(s) 384
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 1 cut(s) 132
BstMAI GTCTC 1 cut(s) 482
BstMBI GATC 1 cut(s) 129
BstMWI GCNNNNNNNGC 3 cut(s) 380, 407, 599
BstNI CCWGG 2 cut(s) 302, 513
BstSCI CCNGG 2 cut(s) 300, 511
BstSLI GKGCMC 1 cut(s) 558
BstV2I GAAGAC 1 cut(s) 125
BsuRI GGCC 3 cut(s) 149, 319, 602
BtgI CCRYGG 2 cut(s) 262, 375
BtsCI GGATG 2 cut(s) 365, 490
BtsIMutI CAGTG 2 cut(s) 180, 588
Cac8I GCNNGC 1 cut(s) 206
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 2 cut(s) 163, 601
CseI GACGC 2 cut(s) 119, 199
CviAII CATG 6 cut(s) 235, 263, 376, 405, 545, 598
DdeI CTNAG 1 cut(s) 580
DpnI GATC 1 cut(s) 131
DpnII GATC 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 262, 375
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 163
Eco88I CYCGRG 1 cut(s) 616
EcoNI CCTNNNNNAGG 1 cut(s) 104
EcoRII CCWGG 2 cut(s) 300, 511
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 2 cut(s) 262, 375
ErhI CCWWGG 2 cut(s) 262, 375
FaeI CATG 6 cut(s) 238, 266, 379, 408, 548, 601
FaqI GGGAC 3 cut(s) 424, 579, 659
FatI CATG 6 cut(s) 234, 262, 375, 404, 544, 597
Fnu4HI GCNGC 1 cut(s) 56
FokI GGATG 2 cut(s) 352, 497
Fsp4HI GCNGC 1 cut(s) 56
GlaI GCGC 1 cut(s) 382
GluI GCNGC 1 cut(s) 56
GsuI CTGGAG 1 cut(s) 495
HaeII RGCGCY 1 cut(s) 384
HaeIII GGCC 3 cut(s) 149, 319, 602
HapII CCGG 2 cut(s) 153, 209
HgaI GACGC 2 cut(s) 119, 199
HhaI GCGC 1 cut(s) 383
Hin1I GRCGYC 2 cut(s) 111, 191
Hin1II CATG 6 cut(s) 238, 266, 379, 408, 548, 601
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HinfI GANTC 1 cut(s) 70
HpaII CCGG 2 cut(s) 153, 209
Hpy188I TCNGA 2 cut(s) 370, 683
Hpy188III TCNNGA 2 cut(s) 39, 618
Hpy99I CGWCG 2 cut(s) 86, 98
HpyAV CCTTC 3 cut(s) 330, 569, 580
HpyCH4III ACNGT 3 cut(s) 52, 175, 347
HpyCH4IV ACGT 2 cut(s) 428, 678
HpyCH4V TGCA 2 cut(s) 238, 544
HpyF10VI GCNNNNNNNGC 3 cut(s) 380, 407, 599
HpyF3I CTNAG 1 cut(s) 580
HpySE526I ACGT 2 cut(s) 428, 678
Hsp92I GRCGYC 2 cut(s) 111, 191
Hsp92II CATG 6 cut(s) 238, 266, 379, 408, 548, 601
HspAI GCGC 1 cut(s) 381
Kzo9I GATC 1 cut(s) 129
LmnI GCTCC 1 cut(s) 590
MaeII ACGT 2 cut(s) 428, 678
MaeIII GTNAC 1 cut(s) 446
MalI GATC 1 cut(s) 131
MboI GATC 1 cut(s) 129
MboII GAAGA 2 cut(s) 130, 478
MhlI GDGCHC 1 cut(s) 558
MluCI AATT 2 cut(s) 133, 270
MlyI GAGTC 1 cut(s) 79
MnlI CCTC 6 cut(s) 100, 152, 204, 238, 334, 625
MslI CAYNNNNRTG 2 cut(s) 261, 483
MspI CCGG 2 cut(s) 153, 209
MspR9I CCNGG 2 cut(s) 302, 513
Mva1269I GAATGC 1 cut(s) 460
MvaI CCWGG 2 cut(s) 302, 513
MwoI GCNNNNNNNGC 3 cut(s) 380, 407, 599
NcoI CCATGG 2 cut(s) 262, 375
NdeII GATC 1 cut(s) 129
NlaIII CATG 6 cut(s) 238, 266, 379, 408, 548, 601
NlaIV GGNNCC 5 cut(s) 65, 226, 257, 557, 603
NmeAIII GCCGAG 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 460
PflFI GACNNNGTC 1 cut(s) 493
PkrI GCNGC 1 cut(s) 57
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
Psp6I CCWGG 2 cut(s) 300, 511
PspGI CCWGG 2 cut(s) 300, 511
PspN4I GGNNCC 5 cut(s) 65, 226, 257, 557, 603
PspPI GGNCC 2 cut(s) 163, 601
PsyI GACNNNGTC 1 cut(s) 493
RseI CAYNNNNRTG 2 cut(s) 261, 483
SatI GCNGC 1 cut(s) 56
Sau3AI GATC 1 cut(s) 129
Sau96I GGNCC 2 cut(s) 163, 601
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 2 cut(s) 302, 513
SduI GDGCHC 1 cut(s) 558
SetI ASST 9 cut(s) 102, 144, 230, 431, 517, 561, 581, 595, 681
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 2 cut(s) 261, 483
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 2 cut(s) 133, 270
SsiI CCGC 3 cut(s) 56, 166, 458
StyD4I CCNGG 2 cut(s) 300, 511
StyI CCWWGG 2 cut(s) 262, 375
TaaI ACNGT 3 cut(s) 52, 175, 347
TaiI ACGT 2 cut(s) 431, 681
TaqI TCGA 2 cut(s) 93, 628
TasI AATT 2 cut(s) 133, 270
TauI GCSGC 1 cut(s) 58
TscAI CASTG 2 cut(s) 180, 588
TspDTI ATGAA 4 cut(s) 263, 282, 467, 561
TspRI CASTG 2 cut(s) 180, 588
Tth111I GACNNNGTC 1 cut(s) 493
VpaK11BI GGWCC 1 cut(s) 163
XagI CCTNNNNNAGG 1 cut(s) 104
XcmI CCANNNNNNNNNTGG 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.