FvH4_6g50000

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000309
Physical Location & Seq
Reverse (-)
37307806 .. 37308993
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50000.t1

Sequence Viewer

Length: 816 bp
ATGGCATCAGAATCTAATCTTTCTCTCTTCACTATCACACTTCTCTTGGCCCTTCTTACTATCCTCTCTCATGCAACTGCTTCTCATCCTCACAACAAGAGCTCTTCCCCATTTGAGTTCCTTGAGCATCTCAAAGGTTGCCACAAAGGTGAAAAGGTCAAGGGTATCAATGATCTCAAAAAATACCTTGAAAAGTACGGTTACTTGAACTACAAAACCAACCAGTGTCATTTGGACGACGACGATTTCGACGACCGTTTGGCGGAAGCCGTCAAGACTTACCAGATCAATTACCATCTCAAGTCCACCGGAACACTGGATGGCAAAACAGTGTTGAAGATGATGACGCCTCGTTGTGGCGTGCCGGACATCATCAATGGCACCTCGTCGATGCGATCAGCCAAGAAAAGGCACCACCATGGCTCTGTTGCACGGGCATTCGCAACGTGGGCTGCGAGCACTCACTTCACGTTCAGCCTCACCCAAAGTTACGAAAATGCGGATCTGAAAATTAGTTTTGAGAAGGGTGATCATGGAGACGGGCATGCATTTGATGGACCTGGCGGAGTGCTGGCCCATGCTGTTAGCTCGTTGCATGGAATATTCCACTACGACGCAGATGAGACATGGTCTGCTGGTGCTAAACCAGGTGCTGTTGACATGGAGACGGTGGCTCTGCATGAAATAGGGCACCTTCTTGGACTGGGACATAGCTCAGTGGAGGGGGCAATCATGGAACCGATTCTTCCCTCGGGAGTAACTCGAGGTTGGCATGGGGATGATGTTCAAGGTATCAAGGATTTATATAACGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

272

Amino Acids

29.59

Weight (kDa)

6.26

Isoelectric Point (pI)

21.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 56 - 113 2.4e-08 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 134 - 269 5.2e-40 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000309)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g07510 FvH4_6g49990 FvH4_6g50000 FvH4_6g50010
malus_domestica MD04G1045600.v1.1 MD09G1040300.v1.1 MD09G1040400.v1.1 MD09G1040500.v1.1
prunus_persica Prupe.1G198800_v2.0.a1 Prupe.3G277800_v2.0.a1 Prupe.3G278000_v2.0.a1
pyrus_communis pycom17g03670 pycom17g03680 pycom17g03720 pycom17g03730
rosa_chinensis RchiOBHm_Chr2g0170151 RchiOBHm_Chr2g0170161 RchiOBHm_Chr2g0170221 RchiOBHm_Chr2g0170241 RchiOBHm_Chr2g0170251 RchiOBHm_Chr2g0171951 RchiOBHm_Chr4g0400091 RchiOBHm_Chr7g0222561 RchiOBHm_Chr7g0222571
rosa_laevigata RLG00000009215 RLG00000021923 RLG00000021924 RLG00000021929 RLG00000021930 RLG00000021931 RLG00000021932 RLG00000021936 RLG00000021937 RLG00000021938 RLG00000021940 RLG00000021941 RLG00000029406 RLG00000029407
rosa_multiflora Rmu_co8411991.1_g000001 Rmu_co8418277.1_g000001 Rmu_co8459115.1_g000001 Rmu_sc0000037.1_g000004 Rmu_sc0001605.1_g000012 Rmu_sc0001605.1_g000013 Rmu_sc0001605.1_g000018 Rmu_sc0001605.1_g000019 Rmu_sc0012468.1_g000004 Rmu_sc0012468.1_g000011 Rmu_sc0012468.1_g000017 Rmu_sc0012468.1_g000019 Rmu_sc0012468.1_g000026 Rmu_sc0036734.1_g000001 Rmu_sc0036734.1_g000002 Rmu_sc0036735.1_g000001 Rmu_sc0036735.1_g000002
rosa_roxburghii Rroxscaffold_2G00081470 Rroxscaffold_2G00081540 Rroxscaffold_2G00081550 Rroxscaffold_3G00236660 Rroxscaffold_5G00345030 Rroxscaffold_5G00345060
rosa_rugosa Rorug02G0546300 Rorug02G0546800 Rorug02G0546800 Rorug02G0546800 Rorug02G0546900 Rorug02G0547000.1 Rorug02G0547100 Rorug02G0547300 Rorug04G0022500 Rorug07G0210800 Rorug07G0210900
rosa_samantha Rh2AG618400 Rh2AG618700 Rh2AG618800 Rh2AG619000 Rh2AG619100 Rh2AG631500 Rh2AG631600 Rh2BG630000 Rh2BG644800 Rh2BG644900 Rh2CG599400 Rh2CG599500 Rh2CG599600 Rh2CG599700 Rh2DG642000 Rh2DG642200 Rh2DG642300 Rh2DG642400 Rh2DG642500 Rh2DG642800 Rh2DG642900 Rh4AG098000 Rh4BG096300 Rh4CG107400 Rh4DG092900 Rh7AG354000 Rh7AG354100 Rh7AG354500 Rh7AG354700 Rh7BG342900 Rh7CG371500 Rh7CG371600 Rh7DG349300 Rh7DG349400
rosa_wichuraiana Rw0G015130 Rw0G018360 Rw0G018370 Rw2G051210 Rw2G051220 Rw2G051230 Rw2G051240 Rw2G051260 Rw2G051270 Rw4G008170 Rw7G029780 Rw7G030120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 380, 411, 690
AciI CCGC 3 cut(s) 263, 500, 564
AclWI GGATC 1 cut(s) 510
AcyI GRCGYC 1 cut(s) 347
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 3 cut(s) 262, 356, 408
AgsI TTSAA 4 cut(s) 191, 208, 337, 788
AjnI CCWGG 2 cut(s) 559, 646
AleI CACNNNNGTG 1 cut(s) 147
AluBI AGCT 3 cut(s) 102, 588, 714
AluI AGCT 3 cut(s) 102, 588, 714
Alw21I GWGCWC 2 cut(s) 104, 461
Alw26I GTCTC 3 cut(s) 531, 617, 659
AlwI GGATC 1 cut(s) 510
AlwNI CAGNNNCTG 1 cut(s) 653
Ama87I CYCGRG 2 cut(s) 751, 762
AoxI GGCC 2 cut(s) 48, 573
ApeKI GCWGC 1 cut(s) 452
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
Asp700I GAANNNNTTC 1 cut(s) 741
AspS9I GGNCC 3 cut(s) 49, 557, 574
AsuHPI GGTGA 3 cut(s) 161, 472, 539
AvaI CYCGRG 2 cut(s) 751, 762
AvaII GGWCC 1 cut(s) 557
BaeGI GKGCMC 1 cut(s) 693
BanI GGYRCC 3 cut(s) 380, 411, 690
BanII GRGCYC 1 cut(s) 104
Bbv12I GWGCWC 2 cut(s) 104, 461
BbvI GCAGC 1 cut(s) 439
BccI CCATC 3 cut(s) 303, 314, 548
BceAI ACGGC 1 cut(s) 254
BciT130I CCWGG 2 cut(s) 561, 648
BclI TGATCA 1 cut(s) 529
BcoDI GTCTC 3 cut(s) 531, 617, 659
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme1390I CCNGG 2 cut(s) 561, 648
Bme18I GGWCC 1 cut(s) 557
BmeT110I CYCGRG 2 cut(s) 751, 762
BmgT120I GGNCC 3 cut(s) 49, 557, 574
BmiI GGNNCC 4 cut(s) 382, 413, 692, 738
BmrFI CCNGG 2 cut(s) 561, 648
BmrI ACTGGG 1 cut(s) 713
BmsI GCATC 3 cut(s) 14, 136, 381
BmuI ACTGGG 1 cut(s) 713
BpuEI CTTGAG 2 cut(s) 143, 284
BsaHI GRCGYC 1 cut(s) 347
BsaJI CCNNGG 2 cut(s) 418, 750
BsaWI WCCGGW 1 cut(s) 308
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 356, 408
Bse1I ACTGG 3 cut(s) 223, 321, 708
BseBI CCWGG 2 cut(s) 561, 648
BseDI CCNNGG 2 cut(s) 418, 750
BseGI GGATG 3 cut(s) 85, 325, 784
BseLI CCNNNNNNNGG 3 cut(s) 262, 356, 408
BseMII CTCAG 1 cut(s) 729
BseNI ACTGG 3 cut(s) 223, 321, 708
BseSI GKGCMC 1 cut(s) 693
BseXI GCAGC 1 cut(s) 439
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 2 cut(s) 50, 575
BshNI GGYRCC 3 cut(s) 380, 411, 690
BsiEI CGRYCG 1 cut(s) 256
BsiHKAI GWGCWC 2 cut(s) 104, 461
BsiHKCI CYCGRG 2 cut(s) 751, 762
BsiSI CCGG 2 cut(s) 309, 365
BslFI GGGAC 1 cut(s) 720
BslI CCNNNNNNNGG 3 cut(s) 262, 356, 408
BsmAI GTCTC 3 cut(s) 531, 617, 659
BsmBI CGTCTC 2 cut(s) 531, 659
BsmFI GGGAC 1 cut(s) 720
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 2 cut(s) 50, 575
BsoBI CYCGRG 2 cut(s) 751, 762
Bsp1286I GDGCHC 3 cut(s) 104, 461, 693
Bsp143I GATC 5 cut(s) 172, 285, 395, 502, 529
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 3 cut(s) 263, 500, 564
BspANI GGCC 2 cut(s) 50, 575
BspCNI CTCAG 1 cut(s) 728
BspLI GGNNCC 4 cut(s) 382, 413, 692, 738
BspPI GGATC 1 cut(s) 510
BspQI GCTCTTC 1 cut(s) 109
BspT107I GGYRCC 3 cut(s) 380, 411, 690
BsrI ACTGG 3 cut(s) 223, 321, 708
BssECI CCNNGG 2 cut(s) 418, 750
BssMI GATC 5 cut(s) 172, 285, 395, 502, 529
BssNI GRCGYC 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 418
Bst2UI CCWGG 2 cut(s) 561, 648
Bst4CI ACNGT 4 cut(s) 200, 257, 331, 670
Bst6I CTCTTC 2 cut(s) 32, 109
BstACI GRCGYC 1 cut(s) 347
BstC8I GCNNGC 4 cut(s) 362, 457, 546, 573
BstDEI CTNAG 1 cut(s) 715
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 3 cut(s) 85, 325, 784
BstKTI GATC 5 cut(s) 175, 288, 398, 505, 532
BstMAI GTCTC 3 cut(s) 531, 617, 659
BstMBI GATC 5 cut(s) 172, 285, 395, 502, 529
BstMCI CGRYCG 1 cut(s) 256
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 2 cut(s) 561, 648
BstNSI RCATGY 1 cut(s) 548
BstSCI CCNGG 2 cut(s) 559, 646
BstSLI GKGCMC 1 cut(s) 693
BstV1I GCAGC 1 cut(s) 439
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BsuRI GGCC 2 cut(s) 50, 575
BtgI CCRYGG 1 cut(s) 418
BtsCI GGATG 3 cut(s) 85, 325, 784
BtsIMutI CAGTG 4 cut(s) 230, 314, 336, 723
Cac8I GCNNGC 4 cut(s) 362, 457, 546, 573
CaiI CAGNNNCTG 1 cut(s) 653
Cfr13I GGNCC 3 cut(s) 49, 557, 574
CseI GACGC 2 cut(s) 355, 623
CsiI ACCWGGT 1 cut(s) 646
Csp6I GTAC 1 cut(s) 196
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 715
DpnI GATC 5 cut(s) 174, 287, 397, 504, 531
DpnII GATC 5 cut(s) 172, 285, 395, 502, 529
Eam1104I CTCTTC 2 cut(s) 32, 109
EarI CTCTTC 2 cut(s) 32, 109
EciI GGCGGA 2 cut(s) 278, 579
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 418
Eco24I GRGCYC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 557
Eco53kI GAGCTC 1 cut(s) 102
Eco88I CYCGRG 2 cut(s) 751, 762
EcoICRI GAGCTC 1 cut(s) 102
EcoRII CCWGG 2 cut(s) 559, 646
EcoT14I CCWWGG 1 cut(s) 418
EcoT22I ATGCAT 1 cut(s) 550
EcoT38I GRGCYC 1 cut(s) 104
ErhI CCWWGG 1 cut(s) 418
Esp3I CGTCTC 2 cut(s) 531, 659
FaqI GGGAC 1 cut(s) 720
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 3 cut(s) 72, 332, 791
FriOI GRGCYC 1 cut(s) 104
Fsp4HI GCNGC 1 cut(s) 453
GluI GCNGC 1 cut(s) 453
HaeIII GGCC 2 cut(s) 50, 575
HapII CCGG 2 cut(s) 309, 365
HgaI GACGC 2 cut(s) 355, 623
Hin1I GRCGYC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 658
HindII GTYRAC 1 cut(s) 658
HinfI GANTC 2 cut(s) 11, 742
HpaII CCGG 2 cut(s) 309, 365
HphI GGTGA 3 cut(s) 161, 472, 539
Hpy166II GTNNAC 2 cut(s) 306, 658
Hpy188I TCNGA 2 cut(s) 10, 507
Hpy188III TCNNGA 2 cut(s) 274, 753
Hpy8I GTNNAC 2 cut(s) 306, 658
Hpy99I CGWCG 5 cut(s) 242, 245, 254, 391, 617
HpyAV CCTTC 3 cut(s) 62, 517, 704
HpyCH4III ACNGT 4 cut(s) 200, 257, 331, 670
HpyCH4IV ACGT 3 cut(s) 446, 470, 810
HpyCH4V TGCA 5 cut(s) 74, 431, 548, 595, 679
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 1 cut(s) 715
HpySE526I ACGT 3 cut(s) 446, 470, 810
Hsp92I GRCGYC 1 cut(s) 347
Ksp22I TGATCA 1 cut(s) 529
Kzo9I GATC 5 cut(s) 172, 285, 395, 502, 529
LguI GCTCTTC 1 cut(s) 109
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 3 cut(s) 14, 136, 381
MabI ACCWGGT 1 cut(s) 646
MaeII ACGT 3 cut(s) 446, 470, 810
MaeIII GTNAC 3 cut(s) 200, 488, 757
MalI GATC 5 cut(s) 174, 287, 397, 504, 531
MboI GATC 5 cut(s) 172, 285, 395, 502, 529
MboII GAAGA 4 cut(s) 19, 96, 349, 737
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 3 cut(s) 104, 461, 693
MluCI AATT 2 cut(s) 289, 510
MnlI CCTC 8 cut(s) 74, 99, 360, 394, 488, 715, 758, 760
Mph1103I ATGCAT 1 cut(s) 550
MroXI GAANNNNTTC 1 cut(s) 741
MslI CAYNNNNRTG 3 cut(s) 147, 417, 777
MspI CCGG 2 cut(s) 309, 365
MspR9I CCNGG 2 cut(s) 561, 648
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 2 cut(s) 561, 648
MwoI GCNNNNNNNGC 1 cut(s) 449
NcoI CCATGG 1 cut(s) 418
NdeII GATC 5 cut(s) 172, 285, 395, 502, 529
NlaIV GGNNCC 4 cut(s) 382, 413, 692, 738
NsiI ATGCAT 1 cut(s) 550
NspI RCATGY 1 cut(s) 548
OliI CACNNNNGTG 1 cut(s) 147
PaeI GCATGC 1 cut(s) 548
PaeR7I CTCGAG 1 cut(s) 762
PciSI GCTCTTC 1 cut(s) 109
PcsI WCGNNNNNNNCGW 3 cut(s) 246, 249, 452
PctI GAATGC 1 cut(s) 437
PdmI GAANNNNTTC 1 cut(s) 741
PfeI GAWTC 2 cut(s) 11, 742
PflFI GACNNNGTC 1 cut(s) 628
PkrI GCNGC 1 cut(s) 454
Psp124BI GAGCTC 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 559, 646
PspGI CCWGG 2 cut(s) 559, 646
PspN4I GGNNCC 4 cut(s) 382, 413, 692, 738
PspPI GGNCC 3 cut(s) 49, 557, 574
PspXI VCTCGAGB 1 cut(s) 762
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 502
PsyI GACNNNGTC 1 cut(s) 628
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 3 cut(s) 147, 417, 777
SacI GAGCTC 1 cut(s) 104
SapI GCTCTTC 1 cut(s) 109
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 5 cut(s) 172, 285, 395, 502, 529
Sau96I GGNCC 3 cut(s) 49, 557, 574
ScrFI CCNGG 2 cut(s) 561, 648
SduI GDGCHC 3 cut(s) 104, 461, 693
SexAI ACCWGGT 1 cut(s) 646
SfaNI GCATC 3 cut(s) 14, 136, 381
Sfr274I CTCGAG 1 cut(s) 762
SinI GGWCC 1 cut(s) 557
SlaI CTCGAG 1 cut(s) 762
SmiMI CAYNNNNRTG 3 cut(s) 147, 417, 777
SmlI CTYRAG 3 cut(s) 122, 299, 762
SmoI CTYRAG 3 cut(s) 122, 299, 762
SphI GCATGC 1 cut(s) 548
Sse9I AATT 2 cut(s) 289, 510
SsiI CCGC 3 cut(s) 263, 500, 564
SspI AATATT 1 cut(s) 603
SstI GAGCTC 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 559, 646
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 4 cut(s) 200, 257, 331, 670
TaiI ACGT 3 cut(s) 449, 473, 813
TaqI TCGA 3 cut(s) 249, 389, 763
TasI AATT 2 cut(s) 289, 510
TfiI GAWTC 2 cut(s) 11, 742
TscAI CASTG 4 cut(s) 230, 321, 336, 723
TseI GCWGC 1 cut(s) 452
TspDTI ATGAA 1 cut(s) 696
TspRI CASTG 4 cut(s) 230, 321, 336, 723
Tth111I GACNNNGTC 1 cut(s) 628
VpaK11BI GGWCC 1 cut(s) 557
XceI RCATGY 1 cut(s) 548
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XhoI CTCGAG 1 cut(s) 762
XmnI GAANNNNTTC 1 cut(s) 741
Zsp2I ATGCAT 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.